聚焦酶元件进化设计与绿色生物制造领域，致力于发展先进的酶分子改造方法和高通量筛选技术，采用定向进化和半理性设计等手段创制高性能的工业酶和蛋白质，使其获得新属性或提高固有属性；开发简短绿色的化学品合成路径，高效利用可再生廉价替代原料（如：一碳化合物），推动化学品的绿色生物制造；在总结大量实验数据的基础上，从分子水平提炼影响酶/蛋白质特性的一般性规律，结合人工智能手段，为提高工业酶和蛋白质的应用水平提供必要的理论支撑。

071010-生物化学与分子生物学

蛋白质工程

2009-07--2010-09   亚琛工业大学（RWTH Aachen University）   博士学位
2007-07--2009-06   不来梅雅各布大学 （Jacobs University Bremen）   博士学习
2005-09--2007-06   江南大学   硕士学位
2001-09--2005-06   江南大学   本科学位

博士

博士

2016-08~现在, 中国科学院天津工业生物技术研究所, 研究组长
2011-01~2016-07,亚琛工业大学 （RWTH Aachen University）, 研究小组组长 （Subgroup leader）

2016-09~现在, 中国科学院天津工业生物技术研究所, 研究组长
2011-01~2016-07,亚琛工业大学 （亚琛工业大学）, 研究小组组长 （Subgroup leader）

2023年，青年酶工程学家奖 

2023年，天津市自然科学特等奖（第五）

[1] 朱蕾蕾, 聂志华. 一种赖氨酸外排蛋白及其应用. CN: CN116514933A, 2023-08-01.
[2] 朱蕾蕾, 苏晓岚, 杨建花. 漆酶突变体及其应用. CN: CN115710579A, 2023-02-24.
[3] 朱蕾蕾, 石利霞, 高松枫. 一种信号肽突变体及其应用. CN: CN112852800B, 2023-02-03.
[4] 朱蕾蕾, 杨建花, 谭子瑊. 漆酶重组蛋白及其应用. CN: CN114958785A, 2022-08-30.
[5] 朱蕾蕾, 杨建花, 谭子瑊. 一种新的蛋白重组策略及其在酶改造中的应用. CN: CN114958786A, 2022-08-30.
[6] 朱蕾蕾, 李天真, 唐梓静, 谭子瑊. 甲醛转化突变蛋白及其应用. CN: CN114616327A, 2022-06-10.
[7] 朱蕾蕾, 任鹏举, 谭子瑊. 一种芳香族化合物的开环方法. CN: CN114561432A, 2022-05-31.
[8] 朱蕾蕾, 李天真, 谭子瑊, 唐梓静. 一种合成乳酸的方法. CN: CN112852766B, 2022-03-25.
[9] 朱蕾蕾. 一种耐受表面活性剂的脂肪酶变体. 202210198070.7, 2022-03-01.
[10] 朱蕾蕾, 聂志华. 一种赖氨酸外排蛋白及其应用. 202210065236.8, 2022-01-20.
[11] 朱蕾蕾, 李天真, 唐梓静, 谭子瑊, 马延和. 甲醛转化突变蛋白及其应用. CN: CN113832120A, 2021-12-24.
[12] 朱蕾蕾, 聂志华. 一种谷氨酸外排蛋白突变体及其应用. CN: CN113493500A, 2021-10-12.
[13] 朱蕾蕾, 聂志华. 一种生产L-谷氨酸的方法. CN: CN113135985A, 2021-07-20.
[14] 朱蕾蕾, 唐梓静, 蔡韬, 谭子瑊, 孙红兵, 马延和. 一种甲醛转化蛋白及其应用. CN: CN113122525A, 2021-07-16.
[15] 朱蕾蕾, 李天真, 谭子瑊, 唐梓静. 一种合成乳酸的方法. CN: CN112852766A, 2021-05-28.
[16] 朱蕾蕾, 石利霞, 高松枫. 一种信号肽突变体及其应用. CN: CN112852800A, 2021-05-28.
[17] 朱蕾蕾, 成超. 一种溶解性多糖单加氧酶突变体及其应用. CN: CN112442488A, 2021-03-05.
[18] 朱蕾蕾. 抗癌药物精氨酸脱亚胺酶半衰期的分子改造方法及突变株. CN: CN109652400A, 2019-04-19.
[19] 孙志浩, 郑 璞, 刘宇鹏, 朱蕾蕾. 一种微生物发酵生产丁二酸的菌种和方法. CN: CN1814747A, 2006-08-09.

   

[1] Journal of Agriculture and Food Chemistry. 2023, 第 8 作者  通讯作者  
[2] Angewandte Chemie International Edition. 2023, 第 10 作者  通讯作者  
[3] Nan Zhang, Jianhua Yang, Zhimin Li, Junaid Haider, Yingying Zhou, Yu Ji, Schwaneberg, Ulrich, Leilei Zhu. Influences of the Carbohydrate-Binding Module on a Fungal Starch Active Lytic Polysaccharide Monooxygenase. Journal of Agriculture and Food Chemistry[J]. 2023, 第 8 作者  通讯作者  71(47): 18405-18413, 
[4] 王利飞, 谭子瑊, 谢希贤, 朱蕾蕾. 塔格糖-4-异构酶的新酶挖掘及酶学性质研究. 微生物学报[J]. 2023, 第 4 作者63(11): 4197-4207, https://actamicro.ijournals.cn/actamicrocn/article/abstract/20231109?st=article_issue.
[5] Shi, Lixia, Liu, Pi, Tan, Zijian, Zhao, Wei, Gao, Junfei, Gu, Qun, Ma, Hongwu, Liu, Haifeng, Zhu, Leilei. Complete depolymerization of PET wastes by an evolved PET hydrolase from directed evolution. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION[J]. 2023, 第 9 作者  通讯作者  62(14): http://dx.doi.org/10.1002/anie.202218390.
[6] Shi, Lixia, Zhu, Leilei. Recent Advances and Challenges in Enzymatic Depolymerization and Recycling of PET Wastes. CHEMBIOCHEM. 2023, 第 2 作者  通讯作者  http://dx.doi.org/10.1002/cbic.202300578.
[7] Journal of Agricultural and Food Chemistry. 2022, 第 10 作者  通讯作者  
[8] Green Chemistry. 2022, 第 10 作者  通讯作者  
[9] 蔡韬, 刘玉万, 朱蕾蕾, 苏浩, 王钰, 王国坤, 张玲玲, 朱之光, 盛翔, 毕昌昊, 马红武, 田朝光, 张学礼, 吴洽庆, 孙媛霞, 江会锋, 马延和, , , , , , , , , , , , , , , , , . 二氧化碳人工生物转化. 生物工程学报[J]. 2022, 第 3 作者38(11): 4101-4114, https://cjb.ijournals.cn/cjbcn/article/abstract/gc22114101?st=article_issue.
[10] Ma, Yue, Li, Tianzhen, Tan, Zijian, Ma, Long, Liu, Haifeng, Zhu, Leilei. Chemoenzymatic conversion of glycerol to lactic acid and glycolic acid. BIORESOURCES AND BIOPROCESSING[J]. 2022, 第 6 作者  通讯作者  9(1): 
[11] Green Chemistry. 2022, 第 10 作者  通讯作者  
[12] Su, Xiaolan, Yang, Jianhua, Yuan, Huiling, Liu, Cui, Tu, Ran, Liu, Pi, Wang, Qinhong, Zhu, Leilei. Directed Evolution of Laccase for Improved Thermal Stability Facilitated by Droplet-Based Microfluidic Screening System. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2022, 第 8 作者  通讯作者  http://dx.doi.org/10.1021/acs.jafc.2c05048.
[13] Li, Tianzhen, Tan, Zijian, Tang, Zijing, Liu, Pi, Liu, Haifeng, Zhu, Leilei, Ma, Yanhe. One-pot chemoenzymatic synthesis of glycolic acid from formaldehyde. GREEN CHEMISTRY[J]. 2022, 第 6 作者  通讯作者  24(13): 5064-5069, http://dx.doi.org/10.1039/d2gc00688j.
[14] Ren, Pengju, Tan, Zijian, Zhou, Yingying, Tang, Hongzhi, Xu, Ping, Liu, Haifeng, Zhu, Leilei. Biocatalytic CO2 fixation initiates selective oxidative cracking of 1-naphthol under ambient conditions. GREEN CHEMISTRY[J]. 2022, 第 7 作者  通讯作者  24(12): 4766-4771, http://dx.doi.org/10.1039/d2gc01226j.
[15] Critical Reviews in Biotechnology. 2021, 第 10 作者  通讯作者  
[16] Science. 2021, 第 4 作者
[17] Yang, Jianhua, Tu, Ran, Yuan, Huiling, Wang, Qinhong, Zhu, Leilei. Recent advances in droplet microfluidics for enzyme and cell factory engineering. CRITICAL REVIEWS IN BIOTECHNOLOGY[J]. 2021, 第 5 作者  通讯作者  41(7): 1023-1045, http://dx.doi.org/10.1080/07388551.2021.1898326.
[18] Nie, Zhihua, Liu，Pi, Zheng, Ping, Zhu, Leilei. Directed evolution and rational design of mechanosensitive channel MscCG2 for improved glutamate excretion efficiency. Journal of Agricultural and Food Chemistry[J]. 2021, 第 4 作者  通讯作者  
[19] Shi, Lixia, Liu, Haifeng, Gao, Songfeng, Weng, Yunxuan, Zhu, Leilei. Enhanced Extracellular Production of IsPETase in Escherichia coli via Engineering of the pelB Signal Peptide. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY[J]. 2021, 第 5 作者  通讯作者  69(7): 2245-2252, http://dx.doi.org/10.1021/acs.jafc.0c07469.
[20] 黄天晨, 杨建花, 唐梓静, 高乐, 张东远, 侯颖, 朱蕾蕾. 裂解性多糖单加氧酶HcLPMO与纤维素酶协同降解纤维素. 微生物学报[J]. 2021, 第 7 作者61(7): 1971-1982, https://actamicro.ijournals.cn/actamicrocn/article/abstract/20210716?st=article_issue.
[21] Zhihua Nie, Pi Liu, Yu Wang, Xuan Guo, Zijian Tan, Jie Shen, Zijing Tang, Jianping Lin, Jibin Sun, Ping Zheng, Leilei Zhu. Directed Evolution and Rational Design of Mechanosensitive Channel MscCG2 for Improved Glutamate Excretion Efficiency. Journal of Agriculture and Food Chemistry[J]. 2021, 第 11 作者  通讯作者  69: 15660-15669, 
[22] Cai, Tao, Sun, Hongbing, Qiao, Jing, Zhu, Leilei, Zhang, Fan, Zhang, Jie, Tang, Zijing, Wei, Xinlei, Yang, Jiangang, Yuan, Qianqian, Wang, Wangyin, Yang, Xue, Chu, Huanyu, Wang, Qian, You, Chun, Ma, Hongwu, Sun, Yuanxia, Li, Yin, Li, Can, Jiang, Huifeng, Wang, Qinhong, Ma, Yanhe. Cell-free chemoenzymatic starch synthesis from carbon dioxide. SCIENCE[J]. 2021, 第 4 作者373(6562): 1523-+, http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000698977800053.
[23] 杨建花, 苏晓岚, 朱蕾蕾, , , . 高通量筛选系统在定向改造中的新进展. 生物工程学报[J]. 2021, 第 3 作者37(7): 2197-2210, https://cjb.ijournals.cn/cjbcn/article/abstract/gc21072197?st=article_issue.
[24] Guo, Wenzhu, Yang, Jianhua, Huang, Tianchen, Liu, Dandan, Liu, Qian, Li, Jingen, Sun, Wenliang, Wang, Xingji, Zhu, Leilei, Tian, Chaoguang. Synergistic effects of multiple enzymes from industrial Aspergillus niger strain O1 on starch saccharification. BIOTECHNOLOGY FOR BIOFUELS[J]. 2021, 第 9 作者  通讯作者  14(1): http://dx.doi.org/10.1186/s13068-021-02074-x.
[25] Green Chemistry. 2020, 第 10 作者  通讯作者  
[26] 李燕, 杨建花, 李宝库, 朱蕾蕾. 漆酶-介体系统对多种不同结构染料的脱色效果. 环境工程学报[J]. 2020, 第 4 作者14(12): 3308-3316, http://www.cjee.ac.cn/article/doi/10.12030/j.cjee.201912179.
[27] Cheng, Chao, Haider, Junaid, Liu, Pi, Yang, Jianhua, Tan, Zijian, Huang, Tianchen, Lin, Jianping, Jiang, Min, Liu, Haifeng, Zhu, Leilei. Engineered LPMO Significantly Boosting Cellulase-Catalyzed Depolymerization of Cellulose. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY[J]. 2020, 第 10 作者  通讯作者  68(51): 15257-15266, https://www.webofscience.com/wos/woscc/full-record/WOS:000603399600020.
[28] 石利霞, 高松枫, 朱蕾蕾. PET水解酶的研究进展. 生物技术通报[J]. 2020, 第 3 作者  通讯作者  36(10): 226-236, http://lib.cqvip.com/Qikan/Article/Detail?id=7103088614.
[29] Li, Tianzhen, Tang, Zijing, Wei, Hongli, Tan, Zijian, Liu, Pi, Li, Jinlong, Zheng, Yingying, Lin, Jianping, Liu, Weidong, Jiang, Huifeng, Liu, Haifeng, Zhu, Leilei, Ma, Yanhe. Totally atom-economical synthesis of lactic acid from formaldehyde: combined bio-carboligation and chemo-rearrangement without the isolation of intermediates. GREEN CHEMISTRY[J]. 2020, 第 12 作者  通讯作者  22(20): 6809-6814, https://www.webofscience.com/wos/woscc/full-record/WOS:000579757000011.
[30] 聂志华, 朱蕾蕾. 生物素对发酵过程中MscCG外排L-谷氨酸的影响. 生物技术通报[J]. 2020, 第 2 作者36(10): 150-155, http://lib.cqvip.com/Qikan/Article/Detail?id=7103088605.
[31] 李燕, 杨建花, 李宝库, 朱蕾蕾. 定向进化转录调节基因提高漆酶异源表达. 山东化工[J]. 2020, 第 4 作者49(9): 26-28, http://lib.cqvip.com/Qikan/Article/Detail?id=7102048970.
[32] Anand, Deepak, Dhoke, Gaurao V, Gehrmann, Julia, Garakani, Tayebeh M, Davari, Mehdi D, Bocola, Marco, Zhua, Leilei, Schwaneberg, Ulrich. Chiral separation of D/L-arginine with whole cells through an engineered FhuA nanochannel. CHEMICAL COMMUNICATIONS[J]. 2019, 55(38): 5431-5434, https://www.webofscience.com/wos/woscc/full-record/WOS:000468369900029.
[33] Garakani, Tayebeh Mirzaei, Liu, Zhanzhi, Glebe, Ulrich, Gehrmann, Julia, Lazar, Jaroslav, Mertens, Marie Anna Stephanie, Moeller, Mieke, Hamzelui, Niloofar, Zhu, Leilei, Schnakenberg, Uwe, Boeker, Alexander, Schwaneberg, Ulrich. In Situ Monitoring of Membrane Protein Insertion into Block Copolymer Vesicle Membranes and Their Spreading via Potential-Assisted Approach. ACS APPLIED MATERIALS & INTERFACES[J]. 2019, 第 9 作者11(32): 29276-29289, http://dx.doi.org/10.1021/acsami.9b09302.
[34] Liu, Haifeng, Zhu, Leilei, Wallraf, AnneMaria, Raeuber, Christoph, Grande, Philipp M, Anders, Nico, Gertler, Christoph, Werner, Bernd, Klankermayer, Juergen, Leitner, Walter, Schwaneberg, Ulrich. Depolymerization of Laccase-Oxidized Lignin in Aqueous Alkaline Solution at 37 degrees C. ACS SUSTAINABLE CHEMISTRY & ENGINEERING[J]. 2019, 第 2 作者7(13): 11150-11156, http://dx.doi.org/10.1021/acssuschemeng.9b00204.
[35] Cheng, Feng, Yang, Jianhua, Schwaneberg, Ulrich, Zhu, Leilei. Rational surface engineering of an arginine deiminase (an antitumor enzyme) for increased PEGylation efficiency. BIOTECHNOLOGY AND BIOENGINEERING[J]. 2019, 第 4 作者  通讯作者  116(9): 2156-2166, 
[36] Wallraf, AnneMaria, Liu, Haifeng, Zhu, Leilei, Khalfallah, Ghazi, Simons, Christian, Alibiglou, Hoda, Davari, Mehdi D, Schwaneberg, Ulrich. A loop engineering strategy improves laccase lcc2 activity in ionic liquid and aqueous solution. GREEN CHEMISTRY[J]. 2018, 第 3 作者20(12): 2801-2812, https://www.webofscience.com/wos/woscc/full-record/WOS:000435574300013.
[37] Cheng, Feng, Yang, Jianhua, Bocola, Marco, Schwaneberg, Ulrich, Zhu, Leilei. Loop engineering reveals the importance of active-site-decorating loops and gating residue in substrate affinity modulation of arginine deiminase (an anti-tumor enzyme). BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2018, 第 5 作者  通讯作者  499(2): 233-238, http://dx.doi.org/10.1016/j.bbrc.2018.03.134.
[38] Grimm, Alexander R, Sauer, Daniel F, Polen, Tino, Zhu, Leilei, Hayashi, Takashi, Okuda, Jun, Schwaneberg, Ulrich. A Whole Cell E. coli Display Platform for Artificial Metalloenzymes: Poly(phenylacetylene) Production with a Rhodium-Nitrobindin Metalloprotein. ACS CATALYSIS[J]. 2018, 第 4 作者8(3): 2611-2614, https://www.webofscience.com/wos/woscc/full-record/WOS:000426804100111.
[39] Zhao, Jing, FrauenkronMachedjou, Victorine Josiane, Fulton, Alexander, Zhu, Leilei, Davari, Mehdi D, Jaeger, KarlErich, Schwaneberg, Ulrich, Bocola, Marco. Unraveling the effects of amino acid substitutions enhancing lipase resistance to an ionic liquid: a molecular dynamics study. PHYSICAL CHEMISTRY CHEMICAL PHYSICS[J]. 2018, 第 4 作者20(14): 9600-9609, http://dx.doi.org/10.1039/c7cp08470f.
[40] 朱蕾蕾. 3. A Whole Cell E. coli Display Platform for Artificial Metalloenzymes: Poly(phenylacetylene) Production with a Rhodium–Nitrobindin Metalloprotein. ACS Catalysis. 2018, 第 1 作者
[41] FrauenkronMachedjou, Victorine Josiane, Fulton, Alexander, Zhao, Jing, Weber, Lina, Jaeger, KarlErich, Schwaneberg, Ulrich, Zhu, Leilei. Exploring the full natural diversity of single amino acid exchange reveals that 40- 60% of BSLA positions improve organic solvents resistance. BIORESOURCES AND BIOPROCESSING[J]. 2018, 第 7 作者  通讯作者  5(1): https://doaj.org/article/31e9d1d826a94dc7a5f6972aa8e428c1.
[42] Grimm, Alexander R, Sauer, Daniel F, Davari, Mehdi D, Zhu, Leilei, Bocola, Marco, Kato, Shunsuke, Onoda, Akira, Hayashi, Takashi, Okuda, Jun, Schwaneberg, Ulrich. Cavity Size Engineering of a beta-Barrel Protein Generates Efficient Biohybrid Catalysts for Olefin Metathesis. ACS CATALYSIS[J]. 2018, 第 4 作者8(4): 3358-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000430154100082.
[43] 朱蕾蕾. 4. Cavity size engineering of a beta-barrel protein generates efficient biohybrid catalysts for olefin metathesis. ACS Catalysis. 2018, 第 1 作者
[44] Zhao, Jing, FrauenkronMachedjou, Victorine Josiane, Kardashliev, Tsvetan, Ruff, Anna Joelle, Zhu, Leilei, Bocola, Marco, Schwaneberg, Ulrich. Amino acid substitutions in random mutagenesis libraries: lessons from analyzing 3000 mutations. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2017, 第 5 作者101(8): 3177-3187, http://dx.doi.org/10.1007/s00253-016-8035-1.
[45] Markel, Ulrich, Zhu, Leilei, FrauenkronMachedjou, Victorine Josiane, Zhao, Jing, Bocola, Marco, Davari, Mehdi D, Jaeger, KarlErich, Schwaneberg, Ulrich. Are Directed Evolution Approaches Efficient in Exploring Nature's Potential to Stabilize a Lipase in Organic Cosolvents?. CATALYSTS[J]. 2017, 第 2 作者7(5): https://www.webofscience.com/wos/woscc/full-record/WOS:000404099100017.
[46] Himanshu Charan, Julia Kinzel, Ulrich Glebe, Deepak Anand, Tayebeh Mirzaei Garakani, Leilei Zhu, Marco Bocola, Ulrich Schwaneberg, Alexander Böker. Grafting PNIPAAm from β-barrel shaped transmembrane nanopores. BIOMATERIALS. 2016, 第 6 作者107: 115-123, http://dx.doi.org/10.1016/j.biomaterials.2016.08.033.
[47] Cheng, Feng, Zhu, Leilei, Lue, Hongqi, Bernhagen, Juergen, Schwaneberg, Ulrich. Directed arginine deiminase evolution for efficient inhibition of arginine-auxotrophic melanomas. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2015, 第 2 作者  通讯作者  99(3): 1237-1247, https://www.webofscience.com/wos/woscc/full-record/WOS:000348771400019.
[48] Sauer, Daniel F, Bocola, Marco, Broglia, Claudio, Arlt, Marcus, Zhu, LeiLei, Brocker, Melanie, Schwaneberg, Ulrich, Okuda, Jun. Hybrid Ruthenium ROMP Catalysts Based on an Engineered Variant of beta-Barrel Protein FhuA Delta CVFtev : Effect of Spacer Length. CHEMISTRY-AN ASIAN JOURNAL[J]. 2015, 第 5 作者10(1): 177-182, https://www.webofscience.com/wos/woscc/full-record/WOS:000346736100024.
[49] Cheng, Feng, Zhu, Leilei, Schwaneberg, Ulrich. Directed evolution 2.0: improving and deciphering enzyme properties. CHEMICAL COMMUNICATIONS[J]. 2015, 第 2 作者51(48): 9760-9772, https://www.webofscience.com/wos/woscc/full-record/WOS:000355631700001.
[50] Cheng, Feng, Kardashliev, Tsvetan, Pitzler, Christian, Shehzad, Aamir, Lue, Hongqi, Bernhagen, Juergen, Zhu, Leilei, Schwaneberg, Ulrich. A Competitive Flow Cytometry Screening System for Directed Evolution of Therapeutic Enzyme. ACS SYNTHETIC BIOLOGY[J]. 2015, 第 7 作者4(7): 768-775, https://www.webofscience.com/wos/woscc/full-record/WOS:000358398100001.
[51] FrauenkronMachedjou, Victorine Josiane, Fulton, Alexander, Zhu, Leilei, Anker, Carolin, Bocola, Marco, Jaeger, KarlErich, Schwaneberg, Ulrich. Towards Understanding Directed Evolution: More than Half of All Amino Acid Positions Contribute to Ionic Liquid Resistance of Bacillus subtilis Lipase A. CHEMBIOCHEM[J]. 2015, 第 3 作者16(6): 937-945, https://www.webofscience.com/wos/woscc/full-record/WOS:000352489300008.
[52] Zhu, Leilei, Cheng, Feng, Piatkowski, Victoria, Schwaneberg, Ulrich. Protein Engineering of the Antitumor Enzyme PpADI for Improved Thermal Resistance. CHEMBIOCHEM[J]. 2014, 第 1 作者15(2): 276-283, https://www.webofscience.com/wos/woscc/full-record/WOS:000329877400013.
[53] Liu, Haifeng, Zhu, Leilei, Bocola, Marco, Chen, Nora, Spiess, Antje C, Schwaneberg, Ulrich. Directed laccase evolution for improved ionic liquid resistance. GREEN CHEMISTRY[J]. 2013, 第 2 作者15(5): 1348-1355, https://www.webofscience.com/wos/woscc/full-record/WOS:000318296700036.
[54] Zhu, Leilei, Tee, Kang Lan, Roccatano, Danilo, Sonmez, Burcu, Ni, Ye, Sun, ZhiHao, Schwaneberg, Ulrich. Directed Evolution of an Antitumor Drug (Arginine Deiminase PpADI) for Increased Activity at Physiological pH. CHEMBIOCHEM[J]. 2010, 第 1 作者11(5): 691-697, https://www.webofscience.com/wos/woscc/full-record/WOS:000276536000012.
[55] Zhu, Leilei, Verma, Rajni, Roccatano, Danilo, Ni, Ye, Sun, ZhiHao, Schwaneberg, Ulrich. A Potential Antitumor Drug (Arginine Deiminase) Reengineered for Efficient Operation under Physiological Conditions. CHEMBIOCHEM[J]. 2010, 第 1 作者11(16): 2294-2301, https://www.webofscience.com/wos/woscc/full-record/WOS:000284050800012.
[56] 刘宇鹏, 朱蕾蕾, 郑璞, 倪晔, 孙志浩. 琥珀酸发酵高产菌株的选育. 工业微生物[J]. 2007, 第 2 作者37(2): 1-6, http://lib.cqvip.com/Qikan/Article/Detail?id=24238662.
[57] 郑璞, 朱蕾蕾, 刘宇鹏, 徐敏, 孙志浩. 琥珀酸放线杆菌发酵培养基的优化. 工业微生物[J]. 2007, 第 2 作者37(5): 1-5, http://lib.cqvip.com/Qikan/Article/Detail?id=25830133.
[58] 朱蕾蕾, 刘宇鹏, 郑璞, 孙志浩. 一株琥珀酸产生菌的筛选及鉴定. 微生物学通报[J]. 2007, 第 1 作者34(1): 80-84, http://lib.cqvip.com/Qikan/Article/Detail?id=23837272.
[59] 赵丽青, 朱蕾蕾, 孙志浩, 郑璞. 转化异丁香酚生成香草醛纺锤芽孢杆菌的筛选. 微生物学通报[J]. 2006, 第 2 作者33(1): 72-77, http://lib.cqvip.com/Qikan/Article/Detail?id=21207179.

Chapter 3: Channel protein FhuA as a promising biomolecular scaffold for bioconjugates, Bio-Synthetic Hybrid Materials and Bionanoparticles: A Biological Chemical Approach Towards Material Science, Royal Society of Chemistry, 2015-09, 第 1 作者

   

（ 1 ） 中科院****, 主持, 研究所（学校）, 2016-01--2018-12
（ 2 ） 研究组启动经费, 主持, 市地级, 2016-06--2018-06

（1）Directed evolution of arginine deiminase for anti-­tumor application   中德“生物基化学品暨生物炼制”双边研讨会   Leilei Zhu   2015-10-06
（2）Channle protein FhuA: a versatile biomolecular scaffold for biohybrid systems   Leilei Zhu   2015-03-05
（3）Protein engineering of enzymes for improving ionic liquid resistance   中德“生物基化学品暨生物炼制”双边研讨会   Leilei Zhu   2014-10-07