1. Flavivirus and alphavirus replication packaging mechanism.

2. Research and development of new vaccines.

3. Antiviral drug research.

June 2002 – November 2005  Institute for Medical Molecular Biology, University of Luebeck, Germany

    Major: Molecular biology and virology (Ph.D.)  

September 1998 - June 2001  Department of Microbiology, College of Life Sciences, Shandong University, China

    Major: Immunology and Molecular Biology (M.S.)

September 1994 - June 1998  Department of Microbiology, College of Life Sciences, Shandong University, China

                                                Major: Microbiology Engineering (B.S.)

2009-present  Wuhan Institute of Virology, Chinese Academy of Sciences

                      Senior Research Scientist: Flavivirus replication and antiviral development

2008 – 2009  Wadsworth Center, Albany, USA 

                      Research Scientist: Flavivirus replication and antiviral development

2006- 2008   Wadsworth Center, Albany, USA 

                      Postdoctoral research: Flavivirus replication and antiviral development 

2002 –2005   Institute for Medical Molecular Biology, University of Luebeck, Germany 

                      PhD research: Hepatitis A Virus (HAV) replication initiation and cellular protein cleavage during HAV infection

2001 - 2002   Research Associate in the Proteomics Group, Beijing Genomics Institute, Beijing

                      Responsibility: random shotgun cloning, high throughput protein expression and screening

1999 - 2001   Molecular Genetics Key Laboratory, Institute of Biotechnology, Fengtai, Beijing

                      Master research: Recombinant live vaccine of bacterial diarrhea

Biosecurity and ethics

1. Zhang QY, Li XF, Niu X, Li N, Wang HJ, Deng CL, Ye HQ, Huang XY, Chen Q, Xu YP, Dong HL, Li XD, Zhao H, Shi PY, Yuan ZM, Gong P, Fang X, Qin CF*, Zhang B*. Short direct repeats in the 3'-untranslated  region are involved in subgenomic flaviviral RNA production. J Virol. 2020 Feb 28;94(6).
2. Li XD, Deng CL, Yuan ZM, Ye HQ, Zhang B*. Different Degrees of 5'-3' DAR Interactions Modulate Zika Virus Genome Cyclization and Host Specific Replication. J Virol. 2020 Feb 14;94(5).
3. Hong-Lei Zhang#, Hao-Long Dong#, Ya-Nan Zhang, Lin-Lin Xu, Cheng-Lin Deng, Xiao-Feng Li, Xiao-Dan Li, Han-Qing Ye, Zhi-Ming Yuan, Cheng-Feng Qin* & Bo Zhang*. Visualization of chikungunya virus infection in vitro and in vivo. Emerg Microbes Infect. 2019;8(1):1574-1583.
4. Li N, Zhang YN, Deng CL, Shi PY, Yuan ZM, Zhang B*. Replication-defective West Nile virus with NS1 deletion as a new vaccine platform for flavivirus. J Virol. 2019  Aug 13;93(17).
5. Zhang YN, Deng CL, Li JQ, Li N, Zhang QY, Ye HQ, Yuan ZM, Zhang B*. Infectious Chikungunya Virus with a Complete Capsid Deletion: a New Approach for CHIKV Vaccine. J Virol. 2019 Jul 17;93(15).
6. Zhang Q, Li N, Deng C, Zhang Z, Li X, Yoshii K, Ye H, Zhang B. Trans Complementation of Replication-defective Omsk Hemorrhagic Fever Virus for Antiviral Study. Virol Sin. 2019 Aug;34(4):412-422.
7. Liu SQ, Li X, Deng CL, Yuan ZM, Zhang B*.  Development and evaluation of one-step multiplex real-time RT-PCR assay for simultaneous detection of Zika virus and Chikungunya virus. J Med Virol. 2018 Mar;90(3):389-396.
8. Li JQ, Deng CL, Gu D, Li X, Shi L, He J, Zhang QY, Zhang B*, Ye HQ. Development of a replicon cell line-based high throughput antiviral assay for screening inhibitors of Zika virus. Antiviral Res. 2018 ;150:148-154. doi: 10.1016/j.antiviral.
9. Liu SQ, Li X, Zhang YN, Gao AL, Deng CL, Li JH, Jehan S, Jamil N, Deng F, Wei H, Zhang B*. Detection, isolation, and      characterization of chikungunya viruses associated with the Pakistan outbreak of 2016-2017. Virol Sin. 2017;32(6):511-519.
10. Zhang QY, Li XD, Liu SQ, Deng CL, Zhang B, Ye HQ. Development of a stable Japanese encephalitis virus replicon cell line for antiviral screening. Arch Virol. 2017. doi: 10.1007/s00705-017-3508-9.
11. Deng CL, Zhang QY, Chen DD, Liu SQ, Qin CF, Zhang B, Ye HQ. Recovery of the Zika virus  through an in vitro ligation approach. J Gen Virol. 2017; 98(7):1739-1743. doi: 10.1099/jgv.0.000862. Epub 2017 Jul 13.
12. Zhang HL, Ye HQ, Liu SQ, Deng CL, Li XD, Shi PY, Zhang B*. West Nile virus NS1 antagonizes interferon-β production by targeting RIG-I and MDA5. J Virol. 2017 Jun 28. pii: JVI.02396-16. doi: 10.1128/JVI.02396-16.
13. Li XF, Li XD, Deng CL, Dong HL, Zhang QY, Ye Q, Ye HQ, Huang XY, Deng YQ, Zhang B*, Qin CF*. Visualization of a      neurotropic flavivirus infection in mouse reveals unique viscerotropism  controlled by host type I interferon signaling. Theranostics. 2017 Feb 12;7(4):912-925.
14. Zhang HL, Ye  HQ, Deng CL, Liu SQ, Shi PY, Qin CF, Yuan ZM, Zhang B*. Generation and characterization of West Nile pseudo-infectious reporter virus for antiviral screening. Antiviral Res. 2017 May;141:38-47.
15. Wang SR, Zhang QY, Wang JQ, Ge XY, Song YY, Wang YF, Li XD, Fu BS, Xu GH, Shu B, Gong P, Zhang B*, Tian T*, Zhou X*. Chemical Targeting of a  G-Quadruplex RNA in the Ebola Virus L Gene. Cell Chem Biol. 2016 Sep 22;23(9):1113-22.
16. Deng CL, Liu SQ, Zhou DG, Xu LL, Li XD, Zhang PT, Li PH, Ye HQ, Wei HP, Yuan ZM, Qin CF, Zhang B*. Development of   Neutralization Assay Using an eGFP Chikungunya Virus. Viruses. 2016 Jun 28;8(7).
17. Ye J, Chen Z, Li Y, Zhao Z, He W, Zohaib A, Song Y, Deng C, Zhang B, Chen H, Cao S. Japanese Encephalitis Virus NS5 Inhibits Type I Interferon (IFN) Production by Blocking the Nuclear  Translocation of IFN Regulatory Factor 3 and NF-κB. J Virol. 2017 Mar 29;91(8).
18. Xu MY, Liu SQ, Deng CL, Zhang QY, Zhang B*. Detection of Zika virus by SYBR green one-step real-time RT-PCR. J Virol Methods. 2016 Oct;236:93-7.
19. Deng C, Liu S, Zhang Q, Xu M, Zhang H, Gu D, Shi L, He J, Xiao G,  Zhang B*. Isolation and characterization of Zika virus imported to China using C6/36 mosquito cells. Virol Sin. 2016;31(2):176-9.
20. Li XD, Deng CL, Ye HQ, Zhang HL, Zhang QY, Chen DD, Zhang PT, Shi PY, Yuan ZM, Zhang B*. Transmembrane Domains of NS2B Contribute to Both Viral RNA Replication and Particle Formation in Japanese Encephalitis Virus. J Virol. 2016 Apr 6. pii: JVI.00340-16
21. Liu SQ, Zhang B*. Zika virus: a flavivirus caused pandemics in Latin America. Virol Sin. 2016;31(2):101-2.
22. Zhang PT, Shan C, Li XD, Liu SQ, Deng CL, Ye HQ, Shang BD, Shi PY, Lv M, Shen BF, Qin CF, Zhang B*. Generation of a recombinant West Nile virus  stably expressing the Gaussia luciferase for neutralization assay. Virus Res. 2016; 211:17-24.
23. Deng C, Li X, Liu S, Xu L, Ye H, Qin CF, Zhang B*. Development and characterization of a clinical  strain of Coxsackievirus A16 and an eGFP infectious clone. Virol Sin. 2015; 30(4):269-76.
24. Liu SQ, Rayner S, Zhang B*. How Ebola has been evolving in West Africa. Trends Microbiol. 2015;23(7):387-8.
25. Xu LL, Shan C, Deng CL, Li XD, Shang BD, Ye HQ, Liu SQ, Yuan ZM, Wang QY, Shi PY*, Zhang B*. Development of a stable Gaussia luciferase  enterovirus 71 reporter virus. J Virol Methods. 2015;219:62-6.
26. Liu SQ, Deng CL, Yuan ZM, Rayner S, Zhang B*. Identifying the pattern of molecular evolution for Zaire ebolavirus in the 2014 outbreak in West Africa. Infect Genet Evol. 2015; 32:51-59.
27. Li XD, Ye HQ, Deng CL, Liu SQ, Zhang HL, Shang BD, Shi PY, Yuan ZM, Zhang B*. Genetic interaction between NS4A and NS4B for replication of Japanese encephalitis virus. J Gen Virol. 2015;96(Pt  6):1264-75.
28. Deng CL, Yeo H, Ye HQ, Liu SQ, Shang BD, Gong P, Alonso S, Shi PY*, Zhang B*. Inhibition of enterovirus 71 by adenosine analog NITD008. J Virol. 2014;88(20):11915-23.
29. Li XD, Shan C, Deng CL, Ye HQ, Shi PY, Yuan ZM, Gong P*, Zhang B*. The interface between methyltransferase and polymerase of NS5 is essential for  flavivirus replication. PLoS  Negl Trop Dis. 2014;8(5):e2891.
30. Li XD, Li XF, Ye HQ, Deng CL, Ye Q, Shan C, Shang  BD, Xu LL, Li SH, Cao S, Yuan Z, Shi PY, Qin CF*, Zhang B*. Recovery of a chemically synthesized Japanese encephalitis virus  revealed two critical adaptive mutations in NS2B and NS4A. J Gen Virol. 2013; 95(Pt 4):806-15.
31. Shan C, Li X, Deng C, Shang B, Xu L, Ye H, Yuan Z*, Zhang B*. Development and characterization of West Nile virus replicon expressing secreted Gaussia luciferase. Virol Sin. 2013; 28(3):161-6.
32. Chen C, Wang Y, Shan C, Sun Y, Xu P, Zhou H, Yang C, Shi PY, Rao Z, Zhang B*, Lou Z*. Crystal structure of EV71 RNA-dependent RNA polymerase complexed with its protein primer VPg: Implication for a trans mechanism of VPg uridylylation. J Virol. 2013 May;87(10):5755-68.
33. Li XF, Deng YQ, Zhao H, Ye Q, Wang HJ, Li SH, Zhu SY, Shi PY, Qin ED, Zhang B*, Qin CF*. Noninvasive bioluminescence imaging of dengue virus infection in the brain of A129 mice. Appl Microbiol Biotechnol. 2013 May;97(10):4589-96.Mar 7.
34. Shang B, Deng C, Ye H, Xu W, Yuan Z, Shi PY*, Zhang B*. Development and characterization of a stable eGFP enterovirus 71 for antiviral screening. Antiviral Res. 2013; 97(2):198-205. 
35. Sun Y, Wang Y, Shan C, Chen C, Xu P, Song M, Zhou H, Yang C, Xu W, Shi PY, Zhang B*, Lou Z*. Enterovirus-71 VPg uridylylation uses a two-molecular mechanism of 3Dpol. J Virol. 2012; 86(24):13662-71.

1.     Preparation method and application of the full-length infectious clone of EV71. Patent No: 201110444377.2

2.     Preparation method and application of the full-length infectious clone of EV71 with luciferase gene. Patent No: 201110444376.8

3.     A JEV infectious clone with luciferase gene, construction method and application. Patent No: 201310441707.1

4.     A CA16 infectious clone with eGFP reporter gene, construction method and application. Patent No: 201410077204.5

5.     A Cell line of DV2 replicon with double reporter gene and application. Patent No: 201410104868.6

6.     A CHIKV infectious clone with a complete capsid deletion and the construction method and application in attenuated vaccine. Patent No: 201811504486.7

7.     The construction method and application of replication defective WNV. Patent No: 201910420096.X

8.     A rabies virus infectious clone based on alphavirus replicon vector, construction method and application. Patent No: 201910915961.8

1. Through the study of viral protein structure and function, and virus-host interaction studies, the replication mechanism of flavivirus and alphavirus is analyzed.

2. Use flavivirus and alphavirus as vectors to develop new vaccines against new and highly pathogenic viruses.

3. Screen potential antiviral drugs against flaviviruses and alphaviruses, and conduct corresponding antiviral mechanism research.

Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences

University of Texas Medical Branch

Tsinghua University

Nankai University

已指导学生

单超  博士研究生  071010-生物化学与分子生物学  

商宝娣  博士研究生  071005-微生物学  

徐琳琳  硕士研究生  085238-生物工程  

李晓丹  博士研究生  071005-微生物学  

张红垒  博士研究生  071005-微生物学  

张盼涛  博士研究生  071005-微生物学  

陈冬冬  硕士研究生  071005-微生物学  

张秋艳  博士研究生  071005-微生物学  

李晓  硕士研究生  071005-微生物学  

张哲瑞  硕士研究生  085238-生物工程  

现指导学生

刘静  博士研究生  071010-生物化学与分子生物学  

张亚南  博士研究生  071005-微生物学  

李嘉琪  博士研究生  071005-微生物学  

王冠华  硕士研究生  085238-生物工程  

张哲瑞  博士研究生  100103-病原生物学  

刘鲲鹏  博士研究生  100103-病原生物学  

李娜  博士研究生  100103-病原生物学  

曾湘粤  硕士研究生  100103-病原生物学  

张宏庆  硕士研究生  071005-微生物学  

2019  Award of Outstanding Graduate Instructor in Wuhan education center, Chinese academy of sciences.