General
Yihua Huang, Ph.D, Prof.
National Laboratory of Biomacromolecules, IBP

Email: yihuahuang@sun5.ibp.ac.cn
Telephone: 01064888789
Address: 15 Datun Road, Chaoyang District, Beijing, China
Postcode: 100101

Research Areas

1) membrane protein biogenesis and various protein-conducting channels; 2) biological membrane biogenesis.

Education

Ph.D

Experience

1990-1995 Peking University, China, B.Sc. in Physiology and Biophysics

1995-1998 Peking University, China, M.Sc. in Physiology

1998-2004  Graduate Student, Department of Biochemistry, Weill Medical College of Cornell University, USA
Work Experience
2004-2010.8  Postdoctoral Associate, Department of Biochemistry/HHMI, UT Southwestern Medical Center at Dallas, USA

2010.9-    Investigator, Institute of Biophysics, Chinese Academy of Sciences

Publications

   
Papers

1.    Han L, Zheng J, Wang Y, Yang X, Liu Y, Sun C, Cao B, Zhou H, Ni D, Lou J, Zhao Y, Huang Y*2016Structure of the BAM complex and its implications for biogenesis of outer-membrane proteins.Nat Struct Mol Biol. Mar; 233:192-6 .

2.    Ni D, Huang Y*2015The Expression, Purification, and Structure Determination of BamA from E. coli.Methods Mol Biol. 1329:169-78. 

3.     Cao B, Zhao Y, Kou Y, Ni D, Zhang XC and Huang Y*(2014) Structure of the nonameric bacterial amyloid secretion channel. Proc Natl Acad Sci USA. Dec 16;111(50):E5439-44.

4.    Qiao S, Luo Q, Zhao Y, Zhang XC and Huang Y* (2014) Structural basis for lipopolysaccharide insertion in the bacterial outer membrane  Nature Jul 3; 511(7507):108-11.

5.    Ni D, Yang K and Huang Y* (2014) Refolding, crystallization and preliminary X-ray crystallographic studies of the β-barrel domain of BamA, a membrane protein essential for outer membrane protein biogenesis. Acta Crystallogr F Struct Biol Commun 70(Pt 3):362-5.

6.    Ni D, Wang Y, Yang X, Zhou H, Hou X, Cao B, Lu Z, Zhao X, Yang K and Huang Y* (2014) Structural and functional analysis of the β-barrel domain of BamA from Escherichia coli. FASEB J 28(6):2677-2685.

7.    Huang Y*, Smith B., Chen L, Baxter R & Deisenhofer J* (2009) Insights into pilus assembly and secretion from the structure and functional characterization of usher PapC. Proc Natl Acad Sci USA, 106(18): 7403-7.

8.    Huang Y, Baxter R, Smith BS, Partch CL, Colbert CL and Deisenhofer J (2006) Crystal structure of cryptochrome 3 from Arabidopsis thaliana and its implication for photolyase activity. Proc Natl Acad Sci USA, 103(47): 17701-6

9.    Huang Y, Park YC, Rich RL, Segal D, Myszka D. G. and Wu H (2001). Structural basis of caspase inhibition by XIAP: differential roles of the linker versus the BIR domain. Cell, 104: 781-790.

10.Huang Y, Rich R., Myszkat D. and Wu H. (2003) Requirement of both the second and third BIR domains for the relief of X-linked inhibitor of apoptosis protein (XIAP)-mediated caspase inhibition by Smac. J. Biol. Chem. 278(49): 49517-22

11.Huang Y, Lu M. and Wu H. (2004) Antagonizing XIAP-mediated caspase-3 inhibition: Achilles' heel of cancers? Cancer Cell. 5(1): 1-2

12.Xu G, Cirilli M, Huang Y, Rich RL, Myszka DG, and Wu H (2001). Covalent inhibition revealed by the crystal structure of the p35/caspase-8 complex. Nature, 410: 494-497.

13.Lin SC, Huang Y, Lo YC, Lu M, and Wu H (2007) Crystal structure of the BIR1 domain of XIAP In two crystal forms. Journal of Molecular Biology

14.Lu M Lin S, Huang Y, Kang YJ, Rich R, Lo YC, Myszka D, Han J and Wu H (2007) XIAP induces NF-κB activation via the BIR1/TAB1 interaction and BIR1 dimerization. Molecular Cell 26: 1-14

15.Xu G, Rich RL, Steegborn C, Min T, Huang Y, Myszka DG and Wu H (2003). Mutational analysis of the p35-caspase interaction: A bowstring kinetic model of caspase inhibition by p35. J. Biol. Chem.  278 (7): 5455-61.

16.Wang S, Huang Y, and Zhou Z (1997). Calcium dependence of myocardial hypothermial tolerance on sources of activator. Cryobiology   35 (3): 193-200. 


Students

已指导学生

乔帅  博士研究生  071011-生物物理学  

王研  博士研究生  071011-生物物理学  

寇永君  硕士研究生  085238-生物工程  

现指导学生

骆青山  硕士研究生  071011-生物物理学  

孙传奇  博士研究生  071011-生物物理学  

王成爱  博士研究生  071011-生物物理学  

韩延龙  博士研究生  071011-生物物理学