基本信息
黄亿华 男 博导 生物物理研究所
电子邮件:yihuahuang@sun5.ibp.ac.cn
通信地址:北京市朝阳区大屯路中国科学院生物物理研究所
邮政编码:100101

研究领域

膜蛋白结构生物学

招生信息

2016年拟招硕博连读生2名.
招生专业
071011-生物物理学
招生方向
以X射线衍射晶体学为手段解析参与生物膜生成的膜蛋白结构
膜蛋白结构与功能;膜蛋白生成机制
多糖的生成于转运机制

教育背景

1998-07--2004-02 美国康奈尔大学 博士
1995-09--1998-07 北京大学 理学硕士
1990-09--1995-07 北京大学 理学学士
学历
-- 研究生
学位
-- 博士

工作经历

2010.9-至今,中国科学院生物物理研究所,生物大分子国家重点实验室,研究组长,“****”研究员
2004.3-2010.8,美国德州大学西南医学中心/美国霍华德休斯医学研究所(UT Southwestern Medical Center at Dallas/ HHMI),博士后/助理研究员/讲师

出版信息

1.    Han L, Zheng J, Wang Y, Yang X, Liu Y, Sun C, Cao B, Zhou H, Ni D, Lou J, Zhao Y, Huang Y*2016Structure of the BAM complex and its implications for biogenesis of outer-membrane proteins.Nat Struct Mol Biol. Mar; 233:192-6 .

2.    Ni D, Huang Y*2015The Expression, Purification, and Structure Determination of BamA from E. coli.Methods Mol Biol. 1329:169-78. 

3.     Cao B, Zhao Y, Kou Y, Ni D, Zhang XC and Huang Y*(2014) Structure of the nonameric bacterial amyloid secretion channel. Proc Natl Acad Sci USA. Dec 16;111(50):E5439-44.

4.    Qiao S, Luo Q, Zhao Y, Zhang XC and Huang Y* (2014) Structural basis for lipopolysaccharide insertion in the bacterial outer membrane  Nature Jul 3; 511(7507):108-11.

5.    Ni D, Yang K and Huang Y* (2014) Refolding, crystallization and preliminary X-ray crystallographic studies of the β-barrel domain of BamA, a membrane protein essential for outer membrane protein biogenesis. Acta Crystallogr F Struct Biol Commun 70(Pt 3):362-5.

6.    Ni D, Wang Y, Yang X, Zhou H, Hou X, Cao B, Lu Z, Zhao X, Yang K and Huang Y* (2014) Structural and functional analysis of the β-barrel domain of BamA from Escherichia coli. FASEB J 28(6):2677-2685.

7.    Huang Y*, Smith B., Chen L, Baxter R & Deisenhofer J* (2009) Insights into pilus assembly and secretion from the structure and functional characterization of usher PapC. Proc Natl Acad Sci USA, 106(18): 7403-7.

8.    Huang Y, Baxter R, Smith BS, Partch CL, Colbert CL and Deisenhofer J (2006) Crystal structure of cryptochrome 3 from Arabidopsis thaliana and its implication for photolyase activity. Proc Natl Acad Sci USA, 103(47): 17701-6

9.    Huang Y, Park YC, Rich RL, Segal D, Myszka D. G. and Wu H (2001). Structural basis of caspase inhibition by XIAP: differential roles of the linker versus the BIR domain. Cell, 104: 781-790.

10.Huang Y, Rich R., Myszkat D. and Wu H. (2003) Requirement of both the second and third BIR domains for the relief of X-linked inhibitor of apoptosis protein (XIAP)-mediated caspase inhibition by Smac. J. Biol. Chem. 278(49): 49517-22

11.Huang Y, Lu M. and Wu H. (2004) Antagonizing XIAP-mediated caspase-3 inhibition: Achilles' heel of cancers? Cancer Cell. 5(1): 1-2

12.Xu G, Cirilli M, Huang Y, Rich RL, Myszka DG, and Wu H (2001). Covalent inhibition revealed by the crystal structure of the p35/caspase-8 complex. Nature, 410: 494-497.

13.Lin SC, Huang Y, Lo YC, Lu M, and Wu H (2007) Crystal structure of the BIR1 domain of XIAP In two crystal forms. Journal of Molecular Biology

14.Lu M Lin S, Huang Y, Kang YJ, Rich R, Lo YC, Myszka D, Han J and Wu H (2007) XIAP induces NF-κB activation via the BIR1/TAB1 interaction and BIR1 dimerization. Molecular Cell 26: 1-14

15.Xu G, Rich RL, Steegborn C, Min T, Huang Y, Myszka DG and Wu H (2003). Mutational analysis of the p35-caspase interaction: A bowstring kinetic model of caspase inhibition by p35. J. Biol. Chem.  278 (7): 5455-61.

16.Wang S, Huang Y, and Zhou Z (1997). Calcium dependence of myocardial hypothermial tolerance on sources of activator. Cryobiology   35 (3): 193-200. 


发表论文
[1] 黄亿华. Structures of the BAM complex and its recognition of the β-barrel outer membrane protein (β-OMP) substrates. FASEB J. 2020, [2] 黄亿华. Cryo-EM structure of the nonameric CsgGCsgF. Plos Biology. 2020, [3] 黄亿华. Structure of Yidc from Thermotoga maritima and its implications for Yidc-midiated membrane protein insertion. FASEB J. 2018, [4] Wang, Yan, Pannuri, Archana Andole, Ni, Dongchun, Zhou, Haizhen, Cao, Xiou, Lu, Xiaomei, Romeo, Tony, Huang, Yihua. Structural Basis for Translocation of a Biofilm-supporting Exopolysaccharide across the Bacterial Outer Membrane. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2016, 291(19): 10046-10057, http://dx.doi.org/10.1074/jbc.M115.711762.
[5] Han, Long, Zheng, Jiangge, Wang, Yan, Yang, Xu, Liu, Yanqing, Sun, Chuanqi, Cao, Baohua, Zhou, Haizhen, Ni, Dongchun, Lou, Jizhong, Zhao, Yongfang, Huang, Yihua. Structure of the BAM complex and its implications for biogenesis of outer-membrane proteins. NATURE STRUCTURAL & MOLECULAR BIOLOGY[J]. 2016, 23(3): 192-196, https://www.webofscience.com/wos/woscc/full-record/WOS:000371452500004.
[6] 黄亿华. Structural and functional analysis of the β-barrel domain of BamA from Escherichia coli.. FASEB J. 2014, [7] Ni, Dongchun, Wang, Yan, Yang, Xu, Zhou, Haizhen, Hou, Xiaomin, Cao, Baohua, Lu, Zhixin, Zhao, Xinsheng, Yang, Kun, Huang, Yihua. Structural and functional analysis of the beta-barrel domain of BamA from Escherichia coli. FASEB JOURNAL[J]. 2014, 28(6): 2677-2685, https://www.webofscience.com/wos/woscc/full-record/WOS:000339883600025.
[8] Qiao, Shuai, Luo, Qingshan, Zhao, Yan, Zhang, Xuejun Cai, Huang, Yihua. Structural basis for lipopolysaccharide insertion in the bacterial outer membrane. NATURE[J]. 2014, 511(7507): 108-U523, https://www.webofscience.com/wos/woscc/full-record/WOS:000338199400046.
[9] Dongchun Ni, Kun Yang, Yihua Huang. Refolding, crystallization and preliminary X-ray crystallographic studies of the β-barrel domain of BamA, a membrane protein essential for outer membrane protein biogenesis. Acta Crystallographica. Section F, Structural Biology Communications. 2014, 70(Pt 3): 362-365, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3944703/.
[10] Cao, Baohua, Zhao, Yan, Kou, Yongjun, Ni, Dongchun, Zhang, Xuejun Cai, Huang, Yihua. Structure of the nonameric bacterial amyloid secretion channel. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2014, 111(50): E5439-E5444, https://www.webofscience.com/wos/woscc/full-record/WOS:000346366500016.
[11] Ni, Dongchun, Yang, Kun, Huang, Yihua. Refolding, crystallization and preliminary X-ray crystallographic studies of the beta-barrel domain of BamA, a membrane protein essential for outer membrane protein biogenesis. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS[J]. 2014, 70: 362-365, https://www.webofscience.com/wos/woscc/full-record/WOS:000332405600019.
[12] Huang, Yihua, Smith, Barbara S, Chen, Lucy X, Baxter, Richard H G, Deisenhofer, Johann. Insights into pilus assembly and secretion from the structure and functional characterization of usher PapC. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2009, 106(18): 7403-7407, https://www.webofscience.com/wos/woscc/full-record/WOS:000265783600027.
[13] Miao Lu, Su-Chang Lin, Yihua Huang, Young Jun Kang, Rebecca Rich, Yu-Chih Lo, David Myszka, Jiahuai Han, Hao Wu. XIAP Induces NF-κB Activation via the BIR1/TAB1 Interaction and BIR1 Dimerization. Molecular Cell. 2007, 26(5): 689-702, http://dx.doi.org/10.1016/j.molcel.2007.05.006.
[14] Lin, SuChang, Huang, Yihua, Lo, YuChih, Lu, Miao, Wu, Hao. Crystal structure of the BIR1 domain of XIAP in two crystal forms. JOURNAL OF MOLECULAR BIOLOGY[J]. 2007, 372(4): 847-854, http://dx.doi.org/10.1016/j.jmb.2007.07.019.
[15] 黄亿华. Crystal structure of cryptochrome 3 from Arabidopsis thaliana and its implication for photolyase activity. Proc Natl Acad Sci USA. 2006, [16] Huang, YH, Lu, M, Wu, H. Antagonizing XIAP-mediated caspase-3 inhibition: Achilles' heel of cancers?. CANCER CELL[J]. 2004, 5(1): 1-2, http://dx.doi.org/10.1016/S1535-6108(03)00340-4.
[17] 黄亿华. Smac dimer relieves effector caspase inhibition by binding to the BIR2 and BIR3 domains within each XIAP molecule. J. Biol. Chem. 2003, [18] Huang, YH, Park, YC, Rich, RL, Segal, D, Myszka, DG, Wu, H. Structural basis of caspase inhibition by XIAP: Differential roles of the linker versus the BIR domain. CELL[J]. 2001, 104(5): 781-790, http://dx.doi.org/10.1016/S0092-8674(01)00273-2.
[19] Xu, GZ, Cirilli, M, Huang, YH, Rich, RL, Myszka, DG, Wu, H. Covalent inhibition revealed by the crystal structure of the caspase-8/p35 complex. NATURE[J]. 2001, 410(6827): 494-497, https://www.webofscience.com/wos/woscc/full-record/WOS:000167583800048.
[20] Wang Yan, Andole Pannuri Archana, Ni Dongchun, Zhou Haizhen, Cao Xiou, Lu Xiaomei, Romeo Tony, Huang Yihua. Structural Basis for Translocation of a Biofilm-supporting Exopolysaccharide across the Bacterial Outer Membrane *. The Journal of Biological Chemistry. 291(19): 10046-10057, http://dx.doi.org/10.1074/jbc.M115.711762.

科研活动

1. 国家自然科学基金面上项目(31170698), 项目名称:“菌膜粘附分子聚N-乙酰葡萄糖胺合成与转运的结构生物学研究”,起止时间 2015/01-2018/12,项目主持人,承担经费95万,在研项目。
2. 中国科学院战略性先导科技专项“生物超大分子复合体的结构、功能与调控”项目(XDB08020302)。起止时间: 2014/01-2018/12。子课题名称:“生物膜脂类生成与能量代谢过程”。项目课题骨干成员,承担经费400万元(80万/年)。在研项目。
3. 科技部“国家重大科学研究计划” (973 项目,2013CB910603)。项目名称:“离子通道和膜转运蛋白的结构与功能研究”。起止时间:2013/01-2017/08。项目课题骨干成员,课题名称:“转运膜蛋白的生成,折叠和定位的调控机制研究”,承担经费244.5万元。在研项目。
4. 科技部“国家重大科学研究计划”(973 项目,2012CB917302)。项目名称:“膜蛋白的生成、修饰、组装及质量控制 ”。起止时间:2012/01-2016/08。项目课题负责人,课题名称:“植物叶绿体膜蛋白的转运及组装的分子机制与结构基础”,承担经费248万元。在研项目。
5. 国家自然科学基金面上项目(31470743)。项目名称:“Usher家族外膜蛋白结构与功能研究”。起止时间:2012/01-2015/12,项目主持人,承担经费70万,在研项目。
科研项目
(1) 膜蛋白结构与功能研究,主持,院级级,2010-09--2013-09

指导学生

已指导学生

乔帅  博士研究生  071011-生物物理学  

王研  博士研究生  071011-生物物理学  

寇永君  硕士研究生  085238-生物工程  

现指导学生

骆青山  硕士研究生  071011-生物物理学  

孙传奇  博士研究生  071011-生物物理学  

王成爱  博士研究生  071011-生物物理学  

韩延龙  博士研究生  071011-生物物理学