Molecular mechanism of vertebrate early development by using zebrafish model

Fish genome manipulation and fish directional breeding

02/2005-03/2008:      Post-doctoral research with Profs. Bernard Thisse and Christine Thisse, IGBMC, France

09/1997-10/2002:      Ph.D. study with Prof. Zuoyan Zhu, Institute of Hydrobiology, Chinese Academy of Sciences, China

09/1993-07/1997:      BSc study at the College of Life Science, Wuhan University, China

‍‍‍‍‍‍‍‍‍‍‍‍2016: Science and Technology Award for Chinese Fisheries Youth‍‍

2012: Winner of National Science Fund for Excellent Young Scholars

2011: Elected to the member of Youth Innovation Promotion Association, Chinese Academy of Sciences

2010: Lu Jiaxi Young Talent Prize of Chinese Academy of Sciences (CAS)

2010: Research Excellence Award, Institute of Hydrobiology, CAS

2009: Research Excellence Award, Institute of Hydrobiology, CAS

2008: Research Excellence Award, Institute of Hydrobiology, CAS

2006: FFCSA (La Fondation franco-chinoise pour la science et ses applications) Fellowship Award

2005: Research Excellence Award, Institute of Hydrobiology, CAS

2003: Excellent Doctorate Dissertation Award of Hubei Province, China

2001: Top Class Peng Yingang Award of Chinese Academy of Sciences‍‍‍‍‍‍‍‍‍‍

Research papers:

1.       He, M.-D., Zhang, F.-H., Wang, H.-L., Wang, H.-P., Zhu, Z.-Y. and Sun, Y.-H.* (2015). Efficient ligase 3-dependent microhomology-mediated end joining repair of DNA double-strand breaks in zebrafish embryos. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 780, 86-96.

2.       Pang, S. C., Wang, H. P., Zhu, Z. Y. and Sun, Y. H*. (2015). Transcriptional Activity and DNA Methylation Dynamics of the Gal4/UAS System in Zebrafish. Mar Biotechnol (NY) 17, 593-603.

3.       Dai, Z., Wang, H., Jin, X., Wang, H., He, J., Liu, M., Yin, Z., Sun, Y.* and Lou, Q. (2015). Depletion of suppressor of cytokine signaling-1a causes hepatic steatosis and insulin resistance in zebrafish. Am J Physiol Endocrinol Metab 308, E849-859.

4.       Wei, Z.-Q., Xiong, F., He, M.-D., Wang, H.-P., Zhu, Z.-Y. and Sun, Y.-H.* (2015). Suppression of Ligase 4 or Xrcc6 activities enhances the DNA homologous recombination efficiency in zebrafish primordial germ cells. Acta Hydrobiol Sinica 39, 339-348.

5.       Lu FI^*, Sun YH^*, Wei CY, Thisse C, Thisse B (2014) Tissue Specific Derepression of TCF/LEF Controls the Activity of the Wnt/β-Catenin Pathway. Nature Communications 5: 5368. (co-first author)

6.       Pang SC, Wang HP, Li KY, Zhu ZY, Kang JX, Sun YH* (2014) Double Transgenesis of Humanized fat1 and fat2 Genes Promotes Omega-3 Polyunsaturated Fatty Acids Synthesis in a Zebrafish Model. Mar Biotechnol (NY) 16 (5):580-593.

7.       Wei CY, Wang HP, Zhu ZY, Sun YH* (2014) Transcriptional factors Smad1 and Smad9 act redundantly to mediate zebrafish ventral specification downstream of Smad5. The Journal of Biological Chemistry 289:6604-6618.

8.       Xiong F, Wei ZQ, Zhu ZY, Sun YH* (2013) Targeted Expression in Zebrafish Primordial Germ Cells by Cre/loxP and Gal4/UAS Systems. Mar Biotechnol (NY) 15 (5):526-539.

9.       Wang YW, Wei CY, Dai HP, Zhu ZY, Sun YH* (2013) Subtractive phage display technology identifies zebrafish marcksb that is required for gastrulation. Gene 521 (1):69-77.

10.    Liu CW, Xiong F, Jia HZ, Wang XL, Cheng H, Sun YH*, Zhang XZ, Zhuo RX, Feng J (2013) Graphene-based anticancer nanosystem and its biosafety evaluation using a zebrafish model. Biomacromolecules 14 (2):358-366.

11.    Ahmed A.S. I., Xiong F., Pang S.C., He M.D., Waters M.J., Zhu Z.Y., Sun Y.H.^*, (2011) Activation of GH signaling and GH-independent stimulation of growth in zebrafish by introduction of a constitutively activated GHR construct. Transgenic Research 20(3): 557-567

12.    Ishtiaq Ahmed, A. S., Yu L.Q., Zhu Z.Y., Sun Y.H.^*, (2011) Comparative expression analysis of GHR signaling related factors in zebrafish (Danio rerio) and an in vivo model to study GHR signaling. Acta Hydrobiol Sinica 35(5): 727-738.

13.    Liu Y., Bourgeois C., Pang S., Dreumont N., Kister L., Sun Y.H., Stevenin J., Elliott D.J., (2009) The Germ Cell Nuclear Proteins hnRNP G-T and RBMY Activate a Testis-Specific Exon. PLoS Genetics 5(11): p. e1000707.

14.    Chen C.H., Sun Y.H.*, Pei D.S., Zhu Z.Y., (2009) Comparative expression of zebrafish lats1 and lats2 and their implication in gastrulation movements. Dev Dyn 238(11): 2850-2859.

15.    Luo D., Hu W., Chen S., Xiao Y., Sun Y., Zhu Z., (2009) Identification of differentially expressed genes between cloned and zygote-developing zebrafish embryos at the dome stage using suppression subtractive hybridization. Biol Reprod 80: 674-684.

16.    Pei, D. S., Sun, Y. H.*, Chen, C. H., Chen, S. P., Wang, Y. P., Hu, W. and Zhu, Z. Y. (2008). Identification and characterization of a novel gene differentially expressed in zebrafish cross-subfamily cloned embryos. BMC Dev Biol 8, 29.

17.    Liu, J., Sun, Y. H.*, Wang, Y. W., Wang, N., Pei, D. S., Wang, Y. P., Hu, W. and Zhu, Z. Y. (2008). Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) by cDNA-AFLP. Theriogenology 70, 1525-35.

18.    Pei, D. S., Sun, Y. H.*, Chen, S. P., Wang, Y. P., Hu, W. and Zhu, Z. Y. (2007). Identification of differentially expressed genes from the cross-subfamily cloned embryos derived from zebrafish nuclei and rare minnow enucleated eggs. Theriogenology 68, 1282-91.

19.    Wang, N., Sun, Y. H.*, Liu, J., Wang, Y. P. and Zhu, Z. Y. (2007a). Molecular characterization of common carp (Cyprinus carpio) sonic hedgehog and discovery of its maternal expression. Dev Genes Evol 217, 299-305.

20.    Wang, N., Sun, Y. H.*, Liu, J., Wu, G., Su, J. G., Wang, Y. P. and Zhu, Z. Y. (2007b). Knock down of gfp and no tail expression in zebrafish embryo by in vivo-transcribed short hairpin RNA with T7 plasmid system. J Biomed Sci 14, 767-76.

21.    Liu, J., Sun, Y. H.*, Wang, N., Wang, Y. P. and Zhu, Z. Y. (2006). Cloning, characterization and promoter analysis of common carp hairy/Enhancer-of-split-related gene, her6. J Genet 85, 171-8.

22.    Liu, J., Sun, Y. H.*, Wang, N., Wang, Y. P. and Zhu, Z. Y. (2006b). Upstream regulatory region of zebrafish lunatic fringe: isolation and promoter analysis. Mar Biotechnol (NY) 8, 357-65.

23.    Liu, W. Y., Wang, Y., Sun, Y. H.*, Wang, Y. P., Chen, S. P. and Zhu, Z. Y. (2005). Efficient RNA interference in zebrafish embryos using siRNA synthesized with SP6 RNA polymerase. Dev Growth Differ 47, 323-31.

24.    Sun, Y. H., Chen, S. P., Wang, Y. P., Hu, W. and Zhu, Z. Y. (2005). Cytoplasmic impact on cross-genus cloned fish derived from transgenic common carp (Cyprinus carpio) nuclei and goldfish (Carassius auratus) enucleated eggs. Biol Reprod 72, 510-5.

25.    Wu, B.*, Sun, Y. H.*, Wang, Y. W., Wang, Y. P. and Zhu, Z. Y. (2005). Characterization of transgene integration pattern in F4 hGH-transgenic common carp (Cyprinus carpio L.). Cell Res 15, 447-54. (*: co-first author)

26.    Wu, B.*, Sun, Y. H.*, Wang, Y. P., Wang, Y. W., Zhu, Z. Y.  (2004). Sequences of transgene insertion sites in transgenic F4 common carp. Transgenic Research 13, 95-96. (*Equal contribution)

27.    Sun, Y., Chen, S., Wang, Y. and Zhu, Z. (2000). The onset of foreign gene transcription in nuclear-transferred embryos of fish. Science in China (Series C) 43, 597-605.

Review papers:

28.    Xie, X., Pan, L. and Sun, Y.* (2015). Growing with the world: rapid development of the zebrafish research in China and the China Zebrafish Resource Center. Science China. Life sciences 58, 396-399.

29.    Ye, D., Zhu, Z. and Sun, Y.* (2015). Fish genome manipulation and directional breeding. Science China. Life sciences 58, 170-177.

30.    Sun, Y. H.*, Zhu, Z. Y. (2014) Cross-species cloning: influence of cytoplasmic factors on development. The Journal of Physiology 592:2375-2379.

31.    Wu, G.*, Sun, Y.*, Zhu, Z., (2003) Growth hormone gene transfer in common carp. Aquat. Living Resour  16: 416-420. (*Equal contribution)

32.    Zhu, Z. Y., Sun, Y. H. (2000). Embryonic and genetic manipulation in fish. Cell Research 10, 17-27.

We are focusing on the study of molecular mechanism underlying vertebrate early development by using zebrafish as an animal model, and fish directional breeding by utilizing a combination of genome manipulation and germ cell manipulation techniques. Besides, I am the director of the China Zebrafish Resource Center (CZRC), which is becoming the counterpart of the Zebrafish International Resource Center (ZIRC) in China.

已指导学生

杨明宇  硕士研究生  071007-遗传学  

于力群  硕士研究生  071007-遗传学  

魏志强  硕士研究生  071007-遗传学  

朱林  硕士研究生  071007-遗传学  

张筱帆  博士研究生  071007-遗传学  

黄思雨  硕士研究生  071007-遗传学  

刘成杰  博士研究生  071007-遗传学  

张茹  博士研究生  071007-遗传学  

王亚青  博士研究生  071007-遗传学  

现指导学生

李依  博士研究生  071007-遗传学  

陈正芳  博士研究生  071007-遗传学  

焦圣博  博士研究生  071007-遗传学  

苏乃克  硕士研究生  071007-遗传学  

张泉清  博士研究生  071007-遗传学  

郝永康  博士研究生  071007-遗传学  

何丽佳  硕士研究生  090801-水产养殖  

邓家杰  博士研究生  071007-遗传学  

李雪徽  博士研究生  071007-遗传学  

曾江  硕士研究生  086000-生物与医药