基本信息
孙永华  男  博导  中国科学院水生生物研究所
电子邮件: yhsun@ihb.ac.cn
通信地址: 武汉市东湖南路7号中科院水生所
邮政编码: 430072

研究领域

鱼类发育生物学与生物技术学

招生信息

   
招生专业
071007-遗传学
090801-水产养殖
招生方向
鱼类配子发生与胚胎发育
鱼类遗传育种学

教育背景

1997-09--2002-09   中国科学院水生生物研究所   博士学位
1993-09--1997-06   武汉大学   学士学位
学历
博士研究生
学位
博士

工作经历

2019.3-今:中国科学院水生生物研究所,鱼类发育生物技术学科组组长、国家水生生物种质资源库主任、研究员

2010.12-今:中国科学院水生生物研究所,鱼类发育生物技术学科组组长、国家斑马鱼资源中心主任、研究员

2009. 10- 2009. 12:美国弗吉尼亚大学,细胞生物学系,客座副教授(Visiting Associate Professor)

2006. 9-2008. 3:法国国家科研中心分子细胞及遗传学研究所(IGBMC/CNRS),发育生物学研究中心,法中科学及应用基金会(FFCSA)博士后访问学者

2005. 1 -2010. 11:中国科学院水生生物研究所,副研究员

2002. 7-2004. 12:中国科学院水生生物研究所,助理研究员

社会兼职
2022-06-13-今,国际斑马鱼学会, 理事,亚洲区代表
2020-09-01-今,遗传, 编委
2019-08-01-今,中国动物学会, 理事
2019-01-01-今,激光生物学报, 常务编委
2016-09-23-今,中国动物学会斑马鱼分会, 副理事长兼秘书长
2016-05-31-今,湖北省实验动物学会, 常务理事
2014-12-31-今,湖北省遗传学会, 理事
2014-12-31-今,中国青年科技工作者协会, 会员
2014-12-31-今,中国科学院青年联合会, 委员
2011-12-31-今,中国科学院青年创新促进会, 会员

教授课程

水产遗传育种学
水产生物遗传育种学
水产生物发育生物学
水产动物发育生物生物学
鱼类发育生物学
鱼类基因工程

专利与奖励

   
奖励信息
(1) 国家杰出青年科学基金, 国家级, 2020
(2) 首届中国水产青年科技奖, 国家级, 2016
(3) 中国科学院青年创新促进会优秀会员, , 院级, 2016
(4) 首届国家自然科学基金委优秀青年科学基金, , 国家级, 2012
(5) 中国科学院卢嘉锡青年人才奖, , 院级, 2010
(6) 法中科学及应用基金会FFCSA, , 其他, 2006
专利成果
[1] 叶鼎, 孙永华, 张泉清, 何牡丹, 王厚鹏. 一种可标记鱼类生殖干细胞的多克隆抗体的制备方法. CN: CN113173988A, 2021-07-27.

[2] 潘鲁湲, 张昀, 李玲璐, 李阔宇, 柳力月, 谢训卫, 熊凤, 孙永华. 一种斑马鱼实验管理系统. CN: CN113112221A, 2021-07-13.

[3] 李阔宇, 孙永华. 一种斑马鱼鱼苗养殖的装置. CN: CN211268169U, 2020-08-18.

[4] 袁丽, 杨小红, 李依, 叶鼎, 孙永华, 韩丹翔, 胡强. 螺旋藻γ-亚麻酸的新用途. CN: CN109893520A, 2019-06-18.

[5] 熊凤, 孙永华, 叶鼎, 朱作言. 一种用于原始生殖细胞靶向突变的转基因载体及制备方法和用途. CN: CN105274141B, 2019-04-02.

[6] 孙永华, 庞少臣, 王厚鹏, 朱作言, 康景轩. 一种提高鱼类omega-3多不饱和脂肪酸的方法及应用. 中国: CN103898159A, 2014-07-02.

[7] 孙永华, 熊凤, 魏志强, 朱作言. 一种定向筛选同源重组PGC的载体及构建方法和应用. 中国: CN103397046A, 2013-11-20.

[8] 孙永华, 熊凤, 魏志强, 朱作言. 一种高效标记斑马鱼PGC的载体及转基因鱼的制备方法和用途. 中国: CN103224955A, 2013-07-31.

[9] 孙永华, 朱作言, 熊凤, 伊斯提亚克, 迈克沃特斯. 一种鱼类持续激活生长激素受体基因及制备方法和用途. 中国: CN102241763A, 2011-11-16.

出版信息

   
发表论文
[1] 孙永华. Cyp11a2 Is Essential for Oocyte Development and Spermatogonial Stem Cell Differentiation in Zebrafish. Endocrinology[J]. 2022, [2] Yousef Mohammed, Ding Ye, Mudan He, Houpeng Wang, Yanhua Li, Danxiang Han, Zuoyan Zhu, Yonghua Sun. Induction of biosynthesis of ketocarotenoid from β-carotene in fish embryos. Aquaculture. 2021, 542: http://dx.doi.org/10.1016/j.aquaculture.2021.736863.
[3] 孙永华. Manipulating and Visualizing the Germline with Transgenic lines. Methods in Molecular Biology. 2021, [4] Zhang, Fenghua, Hao, Yongkang, Li, Xianmei, Li, Yi, Ye, Ding, Zhang, Ru, Wang, Xiaosi, He, Mudan, Wang, Houpeng, Zhu, Zuoyan, Sun, Yonghua. Surrogate production of genome-edited sperm from a different subfamily by spermatogonial stem cell transplantation. SCIENCE CHINA-LIFE SCIENCES. 2021, http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000701378400001.
[5] Mohammed, Yousef, Ye, Ding, He, Mudan, Wang, Houpeng, Li, Yanhua, Han, Danxiang, Zhu, Zuoyan, Sun, Yonghua. Induction of biosynthesis of ketocarotenoid from 8-carotene in fish embryos. AQUACULTURE[J]. 2021, 542: [6] Sun, Yonghua, Zhang, Bo, Luo, Lingfei, Shi, DeLi, Wang, Han, Cui, Zongbin, Huang, Honghui, Cao, Ying, Shu, Xiaodong, Zhang, Wenqing, Zhou, Jianfeng, Li, Yun, Du, Jiulin, Zhao, Qingshun, Chen, Jun, Zhong, Hanbing, Zhong, Tao P, Li, Li, Xiong, JingWei, Peng, Jinrong, Xiao, Wuhan, Zhang, Jian, Yao, Jihua, Yin, Zhan, Mo, Xianming, Peng, Gang, Zhu, Jun, Chen, Yan, Zhou, Yong, Liu, Dong, Pan, Weijun, Zhang, Yiyue, Ruan, Hua, Liu, Feng, Zhu, Zuoyan, Meng, Anming, Sun, Yonghua, Pan, Luyuan, Wang, Houpeng, Xie, Weixun, He, Mudan, Ye, Ding, Li, Kuoyu, Xiong, Feng, Liu, Liyue, Li, Linglu, Zhang, Yun, Zhang, Bo, Liu, Da, Cheng, Zhenchao, Hu, Yingying, Wu, Qian, Luo, Zhou, Zhang, Yutian, Wu, Yingdan, Li, Wenyuan, Luo, Lingfei, Ma, Jianlong, Mo, Dashuang, Cai, Pengcheng, Chen, Jinzi, Sun, Junhui, Zhou, Yang, Wu, Chuan, Ni, Rui, Shi, DeLi, Shao, Ming, Zhang, YanJun, Feng, LiJun, Cheng, XiaoNing, Li, JiTong, Xing, YanYi, Zhang, Yan, Meng, YaPing, Yu, BeiBei, Wang, Han, Huang, Jian, Zhang, Shuqing, Ji, Cheng, Tan, Yicheng, Wang, Jingjing, Zhang, Fanmiao, Wang, Mingyong, Huang, Guodong, Zhong, Zhaomin, Zhang, Wei, Cui, Zongbin, Long, Yong, Song, Guili, Li, Xiaohui, Li, Xixi, Chen, Kai, Ren, Jing, Yan, Junjun, Li, Qing, Huang, Honghui, Ruan, Hua, Zhu, Shicheng, Chen, Xuejiao, Pan, Jingjing, Jiang, Faming, Chen, Jiehui, Huang, Chao, Dong, Guoping, Yi, Xiaogui, Cao, Ying, Hu, Ruikun, Huang, Weilai, Liu, Jiangfang, Hong WangXiao, Shu, Dong, Xia, Jianhong, Zhao, Shaoyang, Wang, Pengtao, Wu, Xiuhua, Zhang, Wenqing, Zhang, Yiyue, Xu, Mengchang, Liu, Wei, Huang, Zhibing, Lin, Qing, Wu, Mei, Zhou, Jianfeng, Sheng, Aibo, Tang, Peipei, Gong, Xiaoxia, Mou, Wei, Zu, Congcong, Li, Yun, Lu, Ling, Liu, Yunzhang, Rong, Xiaozhi, Chen, Jianyang, Du, Jiulin, Bu, Jiwen, Du, Xufei, Liu, Tingting, Gu, Shanye, Zhao, Qingshun, Dong, Zhangji, Dong, Xiaohua, Cao, Shasha, Yue, Yunyun, Gu, Chun, Liu, Meijing, Chen, Jun, Gong, Hongjian, Zhong, Hanbing, Yang, Xuanjun, Chen, Jiahao, Xu, Qiushi, Zhong, Tao P, Jin, Daqin, She, Peilu, Sun, Jianjian, Lai, Kaa Seng, Li, Li, Zhang, Yu, Shi, Yanyan, Zhao, Fangying, Xiong, JingWei, Pang, Meijun, Gao, Lu, Lei, Lei, Peng, Jinrong, Lo, Jane, Xiao, Wuhan, Cai, Xiaolian, Ouyang, Gang, Zhang, Jian, Ma, Weirui, Miao, Liyun, Yao, Jihua, Hu, Yu, Yin, Zhan, Mo, Xianming, Li, Xue, Peng, Gang, Zhang, Cuizhen, Wang, Fenghua, Zhu, Jun, Yang, Ruimeng, Yuan, Hao, Chen, Yan, Pan, Yi, Zhou, Yong, Liu, Dong, Zhao, Feng, Pan, Weijun, Liu, Feng, Ding, Yanyan, Xue, Yuanyuan, Wang, Lu, Zhang, Yifan, Ma, Dongyuan, Lu, Xinyan, Gao, Shuai, Xia, Jun, Zhu, Zuoyan, Meng, Anming, Liu, Xingfeng, Qiu, Juhui, Gong, Bo, Chen, Luxi, Xiong, Cong, Yao, Likun, Xing, Cencan, Wang, Yixia, Shen, Weimin, Sun, Jiawei, ZAKOC Consortium. Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9. GENOME RESEARCH[J]. 2020, 30(1): 118-126, https://www.webofscience.com/wos/woscc/full-record/WOS:000506573000011.
[7] Li, Xianmei, Zhang, Fenghua, Wu, Nan, Ye, Ding, Wang, Yaqing, Zhang, Xiaofan, Sun, Yonghua, Zhang, YongAn. A critical role of foxp3a-positive regulatory T cells in maintaining immune homeostasis in zebrafish testis development. JOURNAL OF GENETICS AND GENOMICS[J]. 2020, 47(9): 547-561, http://dx.doi.org/10.1016/j.jgg.2020.07.006.
[8] Feng, Ke, Cui, Xuefan, Song, Yanlong, Tao, Binbin, Chen, Ji, Wang, Jing, Liu, Shaojun, Sun, Yonghua, Zhu, Zuoyan, Trudeau, Vance L, Hu, Wei. Gnrh3 Regulates PGC Proliferation and Sex Differentiation in Developing Zebrafish. ENDOCRINOLOGY[J]. 2020, 161(1): https://www.webofscience.com/wos/woscc/full-record/WOS:000518155500008.
[9] 李阔宇, 潘鲁湲, 孙永华. 斑马鱼鱼房和养殖系统建设标准. 中国比较医学杂志. 2020, 30(6): 121-127, http://lib.cqvip.com/Qikan/Article/Detail?id=7102201984.
[10] Liu, Chengjie, Ye, Ding, Wang, Houpeng, He, Mudan, Sun, Yonghua. Elovl2 But Not Elovl5 Is Essential for the Biosynthesis of Docosahexaenoic Acid (DHA) in Zebrafish: Insight from a Comparative Gene Knockout Study. MARINE BIOTECHNOLOGY[J]. 2020, 22(5): 613-619, https://www.webofscience.com/wos/woscc/full-record/WOS:000565473900001.
[11] Zhang, Qifeng, Ye, Ding, Wang, Houpeng, Wang, Yaqing, Hu, Wei, Sun, Yonghua. Zebrafish cyp11c1 Knockout Reveals the Roles of 11-ketotestosterone and Cortisol in Sexual Development and Reproduction. ENDOCRINOLOGY[J]. 2020, 161(6): https://www.webofscience.com/wos/woscc/full-record/WOS:000540717900002.
[12] 张昀, 潘鲁湲, 李阔宇, 谢训卫, 熊凤, 柳力月, 李玲璐, 孙永华. 斑马鱼常用信息网络及数据库建设进展. 计算机产品与流通. 2020, 121-122, http://lib.cqvip.com/Qikan/Article/Detail?id=00002FUDK9787JP0MPDO5JL16HR.
[13] He, Mudan, Zhang, Ru, Jiao, Shengbo, Zhang, Fenghua, Ye, Ding, Wang, Houpeng, Sun, Yonghua. Nanog safeguards early embryogenesis against global activation of maternal beta-catenin activity by interfering with TCF factors. PLOS BIOLOGY[J]. 2020, 18(7): https://www.webofscience.com/wos/woscc/full-record/WOS:000596578100001.
[14] Mudan He, Ru Zhang, Shengbo Jiao, Fenghua Zhang, Ding Ye, Houpeng Wang, Yonghua Sun. Nanog safeguards early embryogenesis against global activation of maternal β-catenin activity by interfering with TCF factors.. PLoS Biology[J]. 2020, 18(7): https://doaj.org/article/e8b104d5cd564f478f74480005c4a9fa.
[15] 孙永华. Proliferation and Sex Differentiation in Developing Zebrafish.. Endocrinology. 2020, [16] Xianmei Li, Fenghua Zhang, Nan Wu, Ding Ye, Yaqing Wang, Xiaofan Zhang, Yonghua Sun, Yong-An Zhang. A critical role of foxp3a-positive Treg cells in maintaining immune homeostasis in zebrafish testis development.. Journal of Genetics and Genomics[J]. 2020, [17] Jiang, Jue, Wang, Jingchao, Yue, Ming, Cai, Xiaolian, Wang, Tianci, Wu, Chao, Su, Hexiu, Wang, Yanwu, Han, Meng, Zhang, Yingchi, Zhu, Xiaofan, Jiang, Peng, Li, Peng, Sun, Yonghua, Xiao, Wuhan, Feng, Hui, Qing, Guoliang, Liu, Hudan. Direct Phosphorylation and Stabilization of MYC by Aurora B Kinase Promote T-cell Leukemogenesis. CANCER CELL[J]. 2020, 37(2): 200-+, http://dx.doi.org/10.1016/j.ccell.2020.01.001.
[18] Zhang, Fenghua, Li, Xianmei, He, Mudan, Ye, Ding, Xiong, Feng, Amin, Golpour, Zhu, Zuoyan, Sun, Yonghua. Efficient generation of zebrafish maternal-zygotic mutants through transplantation of ectopically induced and Cas9/gRNA targeted primordial germ cells. JOURNAL OF GENETICS AND GENOMICS[J]. 2020, 47(1): 37-47, http://lib.cqvip.com/Qikan/Article/Detail?id=7101666717.
[19] Sun, Yonghua, Zhu, Zuoyan. Designing future farmed fishes using genome editing. SCIENCE CHINA-LIFE SCIENCESnull. 2019, 62(3): 420-422, http://lib.cqvip.com/Qikan/Article/Detail?id=7001721043.
[20] Tian, Jing, Shao, Jinhui, Liu, Cong, Hou, HsinYu, Chou, ChihWei, Shboul, Mohammad, Li, GuoQing, ElKhateeb, Mohammad, Samarah, Omar Q, Kou, Yao, Chen, YuHsuan, Chen, MeiJen, Lyu, Zhaojie, Chen, WeiLeng, Chen, YuFu, Sun, YongHua, Liu, YiWen. Deficiency of lrp4 in zebrafish and human LRP4 mutation induce aberrant activation of Jagged-Notch signaling in fin and limb development. CELLULAR AND MOLECULAR LIFE SCIENCES[J]. 2019, 76(1): 163-178, https://www.webofscience.com/wos/woscc/full-record/WOS:000455367100010.
[21] 黄思雨, 何牡丹, 王厚鹏, 孙永华. 斑马鱼ints12的CRISPR/Cas9敲除及其对UsnRNA剪接的调控. 水生生物学报[J]. 2019, 43(1): 1-8, http://lib.cqvip.com/Qikan/Article/Detail?id=7001048483.
[22] Zhang, Xiaofan, Pang, Shaochen, Liu, Chengjie, Wang, Houpeng, Ye, Ding, Zhu, Zuoyan, Sun, Yonghua. A Novel Dietary Source of EPA and DHA: Metabolic Engineering of an Important Freshwater SpeciesCommon Carp by fat1-Transgenesis. MARINE BIOTECHNOLOGY[J]. 2019, 21(2): 171-185, [23] Ding Ye, Xiaosi Wang, Changyong Wei, Mudan He, Houpeng Wang, Yanwu Wang, Zuoyan Zhu, Yonghua Sun. Marcksb plays a key role in the secretory pathway of zebrafish Bmp2b.. PLoS Genetics[J]. 2019, 15(9): https://doaj.org/article/a49ec30e315046f7b617c2c42e1d0f46.
[24] Ye, Ding, Zhu, Lin, Zhang, Qifeng, Xiong, Feng, Wang, Houpeng, Wang, Xiaosi, He, Mudan, Zhu, Zuoyan, Sun, Yonghua. Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealedby Lifetime Labeling of Germline. MARINE BIOTECHNOLOGY[J]. 2019, 21(2): 217-228, https://www.webofscience.com/wos/woscc/full-record/WOS:000462935700009.
[25] Yonghua Sun, Zuoyan Zhu. Designing future farmed fishes using genome editing. 中国科学:生命科学英文版[J]. 2019, 62(3): 420-422, http://lib.cqvip.com/Qikan/Article/Detail?id=7001721043.
[26] Yang, Xiaohong, Li, Yi, Li, Yanhua, Ye, Ding, Yuan, Li, Sun, Yonghua, Han, Danxiang, Hu, Qiang. Solid Matrix-Supported Supercritical CO2 Enhances Extraction of -Linolenic Acid from the Cyanobacterium Arthrospira (Spirulina) platensis and Bioactivity Evaluation of the Molecule in Zebrafish. MARINE DRUGS[J]. 2019, 17(4): [27] Peng Xu, Jian Xu, Guangjian Liu, Lin Chen, Zhixiong Zhou, Wenzhu Peng, Yanliang Jiang, Zixia Zhao, Zhiying Jia, Yonghua Sun, Yidi Wu, Baohua Chen, Fei Pu, Jianxin Feng, Jing Luo, Jing Chai, Hanyuan Zhang, Hui Wang, Chuanju Dong, Wenkai Jiang, Xiaowen Sun. The allotetraploid origin and asymmetrical genome evolution of the common carp Cyprinus carpio. NATURE COMMUNICATIONS[J]. 2019, 10(1): http://dx.doi.org/10.1038/s41467-019-12644-1.
[28] Chen Minjie, Zhang Jie, Pang Shaochen, Wang Chang, Wang Ling, Sun Yonghua, Song Maoyong, Liang Yong. Evaluating estrogenic and anti-estrogenic effect of endocrine disrupting chemicals (EDCs) by zebrafish ( Danio rerio ) embryo-based vitellogenin 1 ( vtg1 ) mRNA expression. Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology[J]. 2018, [29] 李玲璐, 熊凤, 谢训卫, 潘鲁媛, 李阔宇, 柳力月, 张昀, 孙永华. 国家斑马鱼资源中心的资源、技术和服务建设. 遗传[J]. 2018, 40(8): 683-692, http://lib.cqvip.com/Qikan/Article/Detail?id=7000780180.
[30] Chen, Minjie, Zhang, Jie, Pang, Shaochen, Wang, Chang, Wang, Ling, Sun, Yonghua, Song, Maoyong, Liang, Yong. Evaluating estrogenic and anti-estrogenic effect of endocrine disrupting chemicals (EDCs) by zebrafish (Danio rerio) embryo-based vitellogenin 1 (vtgl) mRNA expression. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY[J]. 2018, 204(1): 45-50, http://www.irgrid.ac.cn/handle/1471x/1760673.
[31] Minjie Chen, Jie Zhang, Shaochen Pang, Chang Wang, Ling Wang, Yonghua Sun, Maoyong Song, Yong Liang. Evaluating estrogenic and anti-estrogenic effect of endocrine disrupting chemicals (EDCs) by zebrafish (Danio rerio) embryo-based vitellogenin 1 (vtg1) mRNA expression. Comparative Biochemistry and Physiology, Part C. 2018, 204: 45-50, http://dx.doi.org/10.1016/j.cbpc.2017.11.010.
[32] Liu, Yanhua, Xue, Weiwei, Zhu, Lin, Ye, Ding, Zhu, Xiaoqin, Wang, Huannan, Sun, Yonghua, Deng, Fengjiao. Nanog suppresses the expression of vasa by directly regulating nlkl in the early zebrafish embryo. BIOCHIMIE[J]. 2017, 142(1): 93-101, http://www.irgrid.ac.cn/handle/1471x/1760657.
[33] Feng, Ke, Luo, Hongrui, Li, Yongming, Chen, Ji, Wang, Yaping, Sun, Yonghua, Zhu, Zuoyan, Hu, Wei. High efficient gene targeting in rice field eel Monopterus albus by transcription activator-like effector nucleases. SCIENCE BULLETINnull. 2017, 62(3): 162-164, http://dx.doi.org/10.1016/j.scib.2017.01.018.
[34] Sun, YongHua. Genome editing opens a new era for physiological study and directional breeding of fishes. SCIENCE BULLETINnull. 2017, 62(3): 157-158, http://dx.doi.org/10.1016/j.scib.2017.01.032.
[35] Yanhua Liu, Weiwei Xue, Lin Zhu, Ding Ye, Xiaoqin Zhu, Huannan Wang, Yonghua Sun, Fengjiao Deng. Nanog suppresses the expression of vasa by directly regulating nlk1 in the early zebrafish embryo. Biochimie. 2017, 142: 93-101, http://dx.doi.org/10.1016/j.biochi.2017.07.014.
[36] 王厚鹏, 张峰华, 黄思雨, 熊凤, 朱作言, 孙永华. 两种密码子优化的Cas9编码基因在斑马鱼胚胎中基因敲除效率的比较. 遗传. 2016, 38(2): 144-154, http://lib.cqvip.com/Qikan/Article/Detail?id=668018707.
[37] Ma, Dongyuan, Xue, Yuanyuan, Zhang, Yifan, Sun, Yonghua, Meng, Anming, Liu, Feng. The rising zebrafish research in China: Meeting report of the 3rd Chinese Zebrafish Principal Investigator Meeting & the Inaugural Meeting of China Zebrafish Society. JOURNAL OF GENETICS AND GENOMICSnull. 2016, 43(10): 617-620, http://dx.doi.org/10.1016/j.jgg.2016.10.001.
[38] Liu, Liyue, Pan, Luyuan, Li, Kuoyu, Zhang, Yun, Zhu, Zuoyan, Sun, Yonghua. Zebrafish Health Conditions in the China Zebrafish Resource Center and 20 Major Chinese Zebrafish Laboratories. ZEBRAFISH[J]. 2016, 13: S8-S18, https://www.webofscience.com/wos/woscc/full-record/WOS:000379775900003.
[39] Wei Zhiqiang, Xiong Feng, He Mudan, Wang Houpeng, Zhu Zuoyan, Sun Yonghua. SUPPRESSION OF LIGASE4 OR XRCC6 ACTIVITIES ENHANCES THE DNA HOMOLOGOUS RECOMBINATION EFFICIENCY IN ZEBRAFISH PRIMORDIAL GERM CELLS. Acta Hydrobiologica Sinica[J]. 2015, 39(2): 339-348, [40] 王华林, 朱作言, 孙永华. 斑马鱼socs2的TALEN敲除及其敲除胚胎的生长研究. 水生生物学报[J]. 2015, 39(4): 831-836, http://lib.cqvip.com/Qikan/Article/Detail?id=665536349.
[41] Xie XunWei, Pan LuYuan, Sun YongHua. Growing with the world: rapid development of the zebrafish research in China and the China Zebrafish Resource Center. SCIENCE CHINA-LIFE SCIENCES[N]. 2015, 58(4): 396-399, http://www.irgrid.ac.cn/handle/1471x/978713.
[42] 魏志强, 熊凤, 何牡丹, 王厚鹏, 朱作言, 孙永华. 抑制Ligase4或Xrcc6活性增强斑马鱼原始生殖细胞中DNA同源重组的效率(英文). 水生生物学报. 2015, 39(2): 339-348, http://lib.cqvip.com/Qikan/Article/Detail?id=664343781.
[43] Ye Ding, Zhu ZuoYan, Sun YongHua. Fish genome manipulation and directional breeding. SCIENCE CHINA-LIFE SCIENCESnull. 2015, 58(2): 170-177, http://dx.doi.org/10.1007/s11427-015-4806-7.
[44] He, MuDan, Zhang, FengHua, Wang, HuaLin, Wang, HouPeng, Zhu, ZuoYan, Sun, YongHua. Efficient ligase 3-dependent microhomology-mediated end joining repair of DNA double-strand breaks in zebrafish embryos. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS[J]. 2015, 780: 86-96, http://dx.doi.org/10.1016/j.mrfmmm.2015.08.004.
[45] Pang, ShaoChen, Wang, HouPeng, Zhu, ZuoYan, Sun, YongHua. Transcriptional Activity and DNA Methylation Dynamics of the Gal4/UAS System in Zebrafish. MARINE BIOTECHNOLOGY[J]. 2015, 17(5): 593-603, http://www.irgrid.ac.cn/handle/1471x/982579.
[46] 李阔宇, 潘鲁湲, 孙永华. 斑马鱼资源的开发保藏与国家斑马鱼资源中心. 中国实验动物学报[J]. 2014, 22(6): 93-98,105, http://lib.cqvip.com/Qikan/Article/Detail?id=663549287.
[47] Lu, FuI, Sun, YongHua, Wei, ChangYong, Thisse, Christine, Thisse, Bernard. Tissue-specific derepression of TCF/LEF controls the activity of the Wnt/beta-catenin pathway. NATURE COMMUNICATIONS[J]. 2014, 5(-): http://www.irgrid.ac.cn/handle/1471x/929329.
[48] Pang, ShaoChen, Wang, HouPeng, Li, KuoYu, Zhu, ZuoYan, Kang, Jing X, Sun, YongHua. Double Transgenesis of Humanized fat1 and fat2 Genes Promotes Omega-3 Polyunsaturated Fatty Acids Synthesis in a Zebrafish Model. MARINE BIOTECHNOLOGY[J]. 2014, 16(5): 580-593, http://dx.doi.org/10.1007/s10126-014-9577-9.
[49] Sun, YongHua, Zhu, ZuoYan. Cross-species cloning: influence of cytoplasmic factors on development. JOURNAL OF PHYSIOLOGY-LONDONnull. 2014, 592(11): 2375-2379, http://www.irgrid.ac.cn/handle/1471x/929353.
[50] 叶鼎, 朱作言, 孙永华. 鱼类基因组操作与定向育种. 中国科学. 生命科学[J]. 2014, 44(12): 1253-1261, [51] Wei, ChangYong, Wang, HouPeng, Zhu, ZuoYan, Sun, YongHua. Transcriptional Factors Smad1 and Smad9 Act Redundantly to Mediate Zebrafish Ventral Specification Downstream of Smad5. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2014, 289(10): 6604-6618, http://dx.doi.org/10.1074/jbc.M114.549758.
[52] 杨明宇, 王厚鹏, 朱作言, 孙永华. 斑马鱼ca15b的克隆及在原始生殖细胞中的特异表达. 水生生物学报[J]. 2014, 38(1): 142-149, http://lib.cqvip.com/Qikan/Article/Detail?id=48542925.
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[54] Liu, ChenWei, Xiong, Feng, Jia, HuiZhen, Wang, XuLi, Cheng, Han, Sun, YongHua, Zhang, XianZheng, Zhuo, RenXi, Feng, Jun. Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model. BIOMACROMOLECULES[J]. 2013, 14(2): 358-366, http://www.irgrid.ac.cn/handle/1471x/733586.
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[67] Liu, Yilei, Bourgeois, Cyril F, Pang, Shaochen, Kudla, Marek, Dreumont, Natacha, Kister, Liliane, Sun, YongHua, Stevenin, James, Elliott, David J. The Germ Cell Nuclear Proteins hnRNP G-T and RBMY Activate a Testis-Specific Exon. PLOS GENETICS[J]. 2009, 5(11): http://ir.ihb.ac.cn/handle/342005/27371.
[68] 孙永华. Identification of differentially expressed genes between cloned and zygote-developing zebrafish embryos at the dome stage using suppression subtractive hybridization.. Biology of Reproduction. 2009, [69] Pei, DeSheng, Sun, YongHua, Zhu, ZuoYan. Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos. MOLECULAR BIOLOGY REPORTS[J]. 2009, 36(6): 1375-1380, http://dx.doi.org/10.1007/s11033-008-9323-3.
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[71] Chen, ChunHong, Sun, YongHua, Pei, DeSheng, Zhu, ZuoYan. Comparative Expression of Zebrafish Lats1 and Lats2 and Their Implication in Gastrulation Movements. DEVELOPMENTAL DYNAMICS[J]. 2009, 238(11): 2850-2859, http://ir.ihb.ac.cn/handle/152342/7502.
[72] ZuoYan Zhu, Wei Hu, YaPing Wang, ShangPing Chen, ChunHong Chen, YongHua Sun, DeSheng Pei. Identification and characterization of a novel gene differentially expressed in zebrafish cross-subfamily cloned embryos. 2008, http://oa.las.ac.cn/oainone/service/browseall/read1?ptype=JA&workid=JA201706298434081ZK.
[73] Pei, DeSheng, Sun, YongHua, Chen, ChunHong, Chen, ShangPing, Wang, YaPing, Hu, Wei, Zhu, ZuoYan. Identification and characterization of a novel gene differentially expressed in zebrafish cross-subfamily cloned embryos. BMC DEVELOPMENTAL BIOLOGY[J]. 2008, 8(1): http://ir.ihb.ac.cn/handle/152342/8198.
[74] Pei, DeSheng, Sun, YongHua, Long, Yong, Zhu, ZuoYan. Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique. MOLECULAR BIOLOGY REPORTS[J]. 2008, 35(2): 139-143, http://ir.ihb.ac.cn/handle/152342/8114.
[75] Pei, DeSheng, Sun, YongHua, Zhu, ZuoYan. Construction of cytoplasmic molecular markers distinguishing Danio rerio from Gobiocypris rarus at high identity domains based on MP-PCR strategy and Sybr Green I detection. MOLECULAR BIOLOGY REPORTS[J]. 2008, 35(1): 45-50, http://ir.ihb.ac.cn/handle/152342/8286.
[76] Wang, Na, Sun, YongHua, Liu, Jing, Wang, YaPing, Zhu, ZuoYan. Molecular characterization of common carp (Cyprinus carpio) Sonic Hedgehog and discovery of its maternal expression. DEVELOPMENT GENES AND EVOLUTION[J]. 2007, 217(4): 299-305, http://ir.ihb.ac.cn/handle/152342/8648.
[77] Wang, Na, Sun, YongHua, Liu, Jing, Wu, Gang, Su, JianGuo, Wang, YaPing, Zhu, ZuoYan. Knock down of gfp and no tail expression in zebrafish embryo by in vivo-transcribed short hairpin RNA with T7 plasmid system. JOURNAL OF BIOMEDICAL SCIENCE[J]. 2007, 14(6): 767-776, http://ir.ihb.ac.cn/handle/152342/8396.
[78] Pei, DeSheng, Sun, YongHua, Chen, ShangPing, Wang, YaPing, Zhu, ZuoYan. Cloning and characterization of cytochrome c oxidase subunit I (COXI) in Gobiocypris rarus. DNA SEQUENCE[J]. 2007, 18(1): 1-8, http://ir.ihb.ac.cn/handle/152342/8684.
[79] 胡炜, 裴得胜, 戴军, 陈尚萍, 孙永华, 汪亚平, 朱作言. 斑马鱼和稀有鲫的SCAR标记建立及其异种克隆胚的检测. 高技术通讯[J]. 2006, 16(9): 959-963, [80] Liu, Jing, Sun, YongHua, Wang, Na, Wang, YaPing, Zhu, ZuoYan. Cloning, characterization and promoter analysis of common carp hairy/Enhancer-of-split-related gene, her6. JOURNAL OF GENETICS[J]. 2006, 85(3): 171-178, http://ir.ihb.ac.cn/handle/152342/8614.
[81] 李凌燕, 柴宝峰, 梁爱华, 孙永华, 王伟. 八肋游仆虫Rab家族新成员Eo—rab-1N基因的克隆与序列分析. 遗传. 2006, 28(4): 437-442, http://lib.cqvip.com/Qikan/Article/Detail?id=21473085.
[82] Liu, Jing, Sun, YongHua, Wang, Na, Wang, YaPing, Zhu, ZuoYan. Upstream regulatory region of zebrafish lunatic fringe: Isolation and promoter analysis. MARINE BIOTECHNOLOGY[J]. 2006, 8(4): 357-365, http://ir.ihb.ac.cn/handle/152342/8878.
[83] Liu, WY, Wang, Y, Sun, YH, Wang, Y, Wang, YP, Chen, SP, Zhu, ZY. Efficient RNA interference in zebrafish embryos using siRNA synthesized with SP6 RNA polymerase. DEVELOPMENT GROWTH & DIFFERENTIATION[J]. 2005, 47(5): 323-331, http://ir.ihb.ac.cn/handle/152342/9218.
[84] Wu, B, Sun, YH, Wang, YW, Wang, YP, Zhu, ZY. Characterization of transgene integration pattern in F4 hGH-transgenic common carp (Cyprinus carpio L.). CELL RESEARCH[J]. 2005, 15(6): 447-454, http://lib.cqvip.com/Qikan/Article/Detail?id=16098188.
[85] 朱作言. Cytoplasmic impact on cross-genus cloned fish derived from transgenic common carp (Cyprinus carpio) nuclei and goldfish (Carassius auratus) enucleated eggs. Biology of reproduction[J]. 2005, 72(3): 510-515, http://ir.ihb.ac.cn/handle/152342/9314.
[86] 茅卫锋, 汪亚平, 孙永华, 吴刚, 陈尚萍, 朱作言. 虹鳟鱼(Oncorhynchus mykiss)组蛋白H3启动子的分子克隆及在稀有鮈鲫(Gobiocypris rarus)中的表达活性分析. 自然科学进展[J]. 2004, 14(1): 46-50, http://lib.cqvip.com/Qikan/Article/Detail?id=9065394.
[87] 茅卫锋, 汪亚平, 孙永华, 吴刚, 陈尚萍, 朱作言. 虹鳟鱼(Oncorhynchus mykiss)组蛋白H_3启动子的分子克隆及在稀有(鱼句)鲫(Gobiocypris rarus)中的表达活性分析. 自然科学进展[J]. 2004, 14(1): 46-50, http://ir.ihb.ac.cn/handle/152342/1910.
[88] Wu, B, Sun, YH, Wang, YP, Wang, YW, Zhu, ZY. Sequences of transgene insertion sites in transgenic F4 common carp. TRANSGENIC RESEARCHnull. 2004, 13(2): 95-96, http://ir.ihb.ac.cn/handle/152342/9502.
[89] 黎双飞, 胡炜, 汪亚平, 孙永华, 陈尚萍, 朱作言. 鲤鱼sGnRH基因克隆及其在成熟个体的表达分析. 遗传学报[J]. 2004, http://ir.ihb.ac.cn/handle/152342/2110.
[90] Li Shuang-Fei (sfli@sohu.com), Hu Wei (Huwei@ihb.ac.cn), Wang Ya-ping, Sun Yong-Hua, Chen Shang-Ping, Zhu Zuo-Yan (zyzhu@ihb.ac.cn). Cloning and expression analysis in mature individuals of salmon gonadotropin-releasing hormone (sGnRH) gene in common carp. Acta Genetica Sinica[J]. 2004, 31(10): 1072-1081, http://www.irgrid.ac.cn/handle/1471x/543338.
[91] 王伟, 孙永华, 汪亚平, 朱作言. 草鱼生长激素在毕赤酵母中的高效分泌表达. 遗传学报. 2003, 30(4): 301-306, http://lib.cqvip.com/Qikan/Article/Detail?id=7579791.
[92] 汪亚平, 胡炜, 吴刚, 孙永华, 陈尚萍, 张甫英, 朱作言, 冯建新, 张西瑞. 转“全鱼”生长激素基因鲤鱼及其F1遗传分析. 科学通报. 2001, 46(3): 226-, http://lib.cqvip.com/Qikan/Article/Detail?id=4942725.
[93] ZHUZUOYAN YONGHUASUN. Embryonic and genetic manipulation in fish. 细胞研究:英文版. 2000, 10(1): 17-27, http://lib.cqvip.com/Qikan/Article/Detail?id=6735877.
[94] 陈尚萍, 汪亚平, 朱作言, 孙永华. 外源基因在鱼类核移植胚胎中的转录起始. 中国科学:C辑. 2000, 30(6): 570-, http://lib.cqvip.com/Qikan/Article/Detail?id=4799746.
[95] 王伟, 汪亚平, 孙永华, 朱作言. 酵母表达的重组草鱼生长激素对鲤鱼的促生长效应. 高技术通讯. 2000, 10(12): 5-, http://lib.cqvip.com/Qikan/Article/Detail?id=4948793.
[96] Sun, YH, Chen, SP, Wang, YP, Zhu, ZY. The onset of foreign gene transcription in nuclear-transferred embryos of fish. SCIENCE IN CHINA SERIES C-LIFE SCIENCES[J]. 2000, 43(6): 597-605, http://ir.ihb.ac.cn/handle/152342/10046.
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[99] 赵浩斌, 陈尚萍, 孙永华, 汪亚平, 朱作言. 外源基因在鱼类胚胎中表达与整合的时序. 科学通报. 1999, 44(22): 2414-, http://lib.cqvip.com/Qikan/Article/Detail?id=3853659.
[100] Sun Yong-Hua, Zhu Zuo-Yan. Cross-species cloning: influence of cytoplasmic factors on development. The Journal of Physiology. 592(Pt 11): 2375-2379, http://www.irgrid.ac.cn/handle/1471x/929353.
发表著作
(1) 基因资源与现代渔业, Gene resources and current fisheries, 中国科学技术出版社, 2010-07, 第 1 作者
(2) 鱼类转基因研究中几个观念的转变和鱼类原始生殖细胞操作, PGC manipulation in fishes, 中国科学技术出版社 , 2012-11, 第 1 作者
(3) Manipulating and Visualizing the Germline with Transgenic Lines, Springer Nature, 2021-08, 第 1 作者

科研活动



科研项目
( 1 ) 基于鱼类分子设计育种的基因操作技术研究, 主持, 国家级, 2010-01--2014-12
( 2 ) 斑马鱼资源的收集、整理和利用, 主持, 部委级, 2012-01--2016-12
( 3 ) 模式动物斑马鱼, 主持, 国家级, 2013-01--2015-12
( 4 ) 原始生殖细胞发育和分化的调控网络研究, 主持, 部委级, 2013-09--2018-07
( 5 ) 中韩生物资源开发和信息交流双边研讨会, 主持, 国家级, 2015-09--2015-12
( 6 ) 中国科学院模式和特色动物实验, 主持, 部委级, 2014-01--2018-06
( 7 ) 国家斑马鱼资源平台, 主持, 省级, 2015-01--2018-12
( 8 ) 国家斑马鱼资源中心运营, 主持, 市地级, 2015-07--2018-12
( 9 ) 功能基因育种潜力的验证与新育种技术研究, 主持, 国家级, 2014-01--2015-12
( 10 ) 鱼类品种改良快速、定向的基因操作技术基础研究, 主持, 国家级, 2004-01--2008-12
( 11 ) 利用基因敲除斑马鱼模型研究Nanog抑制Wnt/β-catenin信号通路的分子调控机制, 主持, 国家级, 2017-01--2020-12
( 12 ) 鱼类分子遗传育种, 参与, 国家级, 2018-01--2023-12
( 13 ) 斑马鱼发育与代谢突变体库的系统创制, 主持, 国家级, 2019-09--2024-08
( 14 ) 利用DHA合成缺陷和富余模型研究DHA对鱼类生殖质形成的作用机制, 主持, 国家级, 2020-01--2023-12
( 15 ) 鱼类生殖细胞发育与生物技术, 主持, 国家级, 2021-01--2025-12

指导学生

已指导学生

杨明宇  硕士研究生  071007-遗传学  

于力群  硕士研究生  071007-遗传学  

魏志强  硕士研究生  071007-遗传学  

朱林  硕士研究生  071007-遗传学  

张筱帆  博士研究生  071007-遗传学  

黄思雨  硕士研究生  071007-遗传学  

刘成杰  博士研究生  071007-遗传学  

张茹  博士研究生  071007-遗传学  

现指导学生

王亚青  博士研究生  071007-遗传学  

李依  博士研究生  071007-遗传学  

陈正芳  博士研究生  071007-遗传学  

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