Project: Amino acids accumulation in tea plants

  Showed using nonaqueous fractionation and molecular methods to determined the different in vivo subcellular sites of L-theanine biosynthesis and hydrolysis in tea plants. This project has found that in tea root tissue, the cytosol was the main site of L-theanine biosynthesis, and cytosol-located CsTSI was the key L-theanine synthase. In tea shoot tissue, L-theanine biosynthesis occurred mainly in the cytosol and chloroplasts and CsGS1.1 and CsGS2 were most likely the key L-theanine synthases. In addition, L-theanine content and distribution were affected by light in leaf tissue.

Project: Pigments accumulation mechanism in banana

  Showed using UPLC-Q-TOF/HPLC and molecular methods the banana pigments accumulation pattern. This project has identified the pigment distribution in banana fruit and revealed the lycopene cyclase enzyme function in banana fruit.

Project: Volatile compounds by plant in response to light and temperature

  Showed using GC-MS and molecular methods that the production of plant volatile compounds responds to light and temperature. This project has found that light can significantly regulate the volatile content in tea leaf and strawberry fruit by activating the genes involved in the corresponding metabolic pathways.

​

• • （1） Effect of the biosynthesis of the volatile compound phenylacetaldehyde on chloroplast modifications in tea (Camellia sinensis) plants, HORTICULTURE RESEARCH, 2023
    （2） 茶叶氨基酸合成代谢及转化研究进展, Research Advances in Amino Acid Metabolism and Transformation of Tea Plants(Camellia sinensis), 中国茶叶, 2022
    （3） Mechanism underlying the carotenoid accumulation in shaded tea leaves, Food Chemistry X, 2022
    （4） Strategies for studying in vivo biochemical formation pathways and multilevel distributions of quality or function-related specialized metabolites in tea (Camellia sinensis), Critical Reviews in Food Science and Nutrition, 2022
    （5） Editorial: Interactions Between Biochemical Pathways Producing Plant Colors and Scents, FRONTIERS IN PLANT SCIENCE, 2022, 
    （6） Transformation of Salicylic Acid and Its Distribution in Tea Plants (Camellia sinensis) at the Tissue and Subcellular Levels, PLANTS-BASEL, 2021
    （7） 茶叶类胡萝卜素合成、代谢调控研究进展, 广东农业科学, 2021
    （8） Stable isotope-labeled precursor tracing reveals that L-alanine is converted to L-theanine via L-glutamate not ethylamine in tea plants in vivo, Journal of Agricultural and Food Chemistry, 2021
    （9） Nonaqueous fractionation and overexpression of fluorescent-tagged enzymes reveals the subcellular sites of L-theanine biosynthesis in tea, PLANT BIOTECHNOLOGY JOURNAL, 2021
    （10） Strategies for studying in vivo biochemical formation pathways and multilevel distributions of quality or function-related specialized metabolites in tea (Camellia sinensis), CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION, 2020
    （11） Molecular Mechanisms Determining the Differential Accumulation of Carotenoids in Plant Species and Varieties, CRITICAL REVIEWS IN PLANT SCIENCES, 2020
    （12） Metabolism of Gallic Acid and Its Distributions in Tea ( Camellia sinensis ) Plants at the Tissue and Subcellular Levels, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020
    （13） Characterization of L‑theanine hydrolase in vitro and subcellular distribution of its specific product ethylamine in tea (Camellia sinensis), JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2020
    （14） Lycopene cyclases determine high alpha-/beta-carotene ratio and increased carotenoids in bananas ripening at high temperatures, FOOD CHEMISTRY, 2019
    （15） Differential accumulation of specialized metabolite L-theanine in green and albino-induced yellow tea (Camellia sinensis) leaves, FOOD CHEMISTRY, 2019
    （16） Microscopic Analyses of Fruit Cell Plastid Development in Loquat ( Eriobotrya japonica ) during Fruit Ripening, MOLECULES, 2019
    （17） Lycopene cyclases determine high α-/β-carotene ratio and increased carotenoids in bananas ripening at high temperatures, FOOD CHEMISTRY, 2019
    （18） Visualized analysis of within-tissue spatial distribution of specialized metabolites in tea (Camellia sinensis) using desorption electrospray ionization imaging mass spectrometry, FOOD CHEMISTRY, 2019
    （19） Comparative analysis of pigments in red and yellow banana fruit, FOOD CHEMISTRY, 2018
    （20） 钟冠报春苣苔花发育进程中花色素苷含量的变化, The Changes of Anthocyanin Content During the Development of Primulina swinglei Flower, 园艺学报, 2018
    （21） Biosynthesis of jasmine lactone in tea (Camellia sinensis) leaves and its formation in response to multiple stresses, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2018
    （22） Formation and emission of linalool in tea ( Camellia sinensis ) leaves infested by tea green leafhopper ( Empoasca ( Matsumurasca ) onukii Matsuda), FOOD CHEMISTRY, 2017
    （23） Formation of (E)-nerolidol in tea (Camellia sinensis) leaves exposed to multiple stresses during tea manufacturing, FOOD CHEMISTRY, 2017
    （24） Formation and emission of linalool in tea (Camellia sinensis) leaves infested by tea green leafhopper (Empoasca (Matsumurasca) onukii Matsuda), FOOD CHEMISTRY, 2017
    （25） Proteolysis of chloroplast proteins is responsible for accumulation of free amino acids in dark-treated tea (Camellia sinensis) leaves, JOURNAL OF PROTEOMICS, 2017
    （26） Differential responses of four biosynthetic pathways of aroma compounds in postharvest strawberry (Fragaria x ananassa Duch.) under interaction of light and temperature, FOOD CHEMISTRY, 2017
    （27） Differential responses of four biosynthetic pathways of aroma compounds in postharvest strawberry (Fragaria × ananassa Duch.) under interaction of light and temperature, FOODCHEMISTRY, 2017
    （28） Analytical method for metabolites involved in biosynthesis of plant volatile compounds, RSC ADVANCES, 2017
    （29） Studies on the Biochemical Formation Pathway of the Amino Acid L-Theanine in Tea (Camellia sinensis) and Other Plants, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2017
    （30） Does oolong tea (Camellia sinensis) made from a combination of leaf and stem smell more aromatic than leaf-only tea? Contribution of the stem to oolong tea aroma, FOOD CHEMISTRY, 2017
    （31） Formation of (E)-nerolidol in tea (Camellia sinensis) leaves exposed to multiple stresses during tea manufacturing, FOOD CHEMISTRY, 2017
    （32） The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato, SCIENTIFIC REPORTS, 2016
    （33） Spatial differences in (Z)-3-hexen-1-ol production preferentially reduces Spodoptera litura larva attack on the young leaves of Nicotiana benthamiana, PLANT SCIENCE, 2016
    （34） Regulation of biosynthesis and emission of volatile phenylpropanoids/benzenoids in petuniax hybrida flowers by multi-factors of circadian clock, light, and temperature, PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2016
    （35） Formation of volatile tea constituent indole during the oolong tea manufacturing process, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2016
    （36） Characterization of functional proteases from flowers of tea (Camellia sinensis) plants, JOURNAL OF FUNCTIONAL FOODS, 2016
    （37） Regulation of biosynthesis and emission of volatile phenylpropanoids/benzenoids in petunia× hybrida flowers by multi-factors of circadian clock, light, and temperature, PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2016
    （38） Recent Advances in the Emission and Functions of Plant Vegetative Volatiles, MOLECULES, 2016
    （39） Dual mechanisms regulating glutamate decarboxylases and accumulation of gamma-aminobutyric acid in tea ( Camellia sinensis ) leaves exposed to multiple stresses, SCIENTIFIC REPORTS, 2016
    （40） Does enzymatic hydrolysis of glycosidically bound volatile compounds really contribute to the formation of volatile compounds during the oolong tea manufacuring process?, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2015
    （41） Regulation of formation of volatile compounds of tea ( Camellia sinensis ) leaves by single light wavelength, SCIENTIFIC REPORTS, 2015
    （42） Involvement of multiple phytoene synthase genes in tissue- and cultivar-specific accumulation of carotenoids in loquat, JOURNAL OF EXPERIMENTAL BOTANY, 2014
    
• • 发表著作