Defense mechanisms of crop plants (maize, tomato, and soybean) against insects; plantparasitic plant interactions.

09.2001-11.2007
Ph.D. Plant Molecular Biology and Genetics
Thesis: molecular study of the trypsin proteinase inhibitor defense mechanism and early herbivoryinduced signaling in Nicotiana
Awarded the Otto Hahn Medal for excellent Ph.D. work in the Max Planck Society, 2008
Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany;
Supervisor: Prof. Ian T.Baldwin

09.1995 -07.1998
M.S. Analytical Chemistry (Chromatography and Mass Spectrometry)
Thesis: application of cyclodextrin-derivative stationary phases in separation of aromatic positional isomers in gas chromatography
Department of Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences,
Dalian, China;
Supervisor: Prof. Daqian Zhu

09.1991-07.1995

B.S. Chemical Engineering
Department of Chemical Engineering, Dalian University of Technology,Dalian, China

04.2012 – present: Full Professor, Kunming Institute of Botany, Chinese Academy of Sciences,
Kunming, China
01.2007 – 04.2012: Group Leader, Department of Molecular Ecology, Max Planck Institute for
Chemical Ecology, Jena, Germany
10.1998 – 08.2001: Research Associate, Modern Analysis Center, Dalian Institute of
Chemical Physics, Chinese Academy of Sciences, Dalian, China

04.2012 – present: Full Professor, Kunming Institute of Botany, Chinese Academy of Sciences,
Kunming, China
01.2007 – 04.2012: Group Leader, Department of Molecular Ecology, Max Planck Institute for
Chemical Ecology, Jena, Germany
10.1998 – 08.2001: Research Associate, Modern Analysis Center, Dalian Institute of
Chemical Physics, Chinese Academy of Sciences, Dalian, China

1. “Plant Molecular Biology”, Kunming Institute of Botany, Chinese Academy of Sciences,Kunming, June, 2013
2. “Transfection of Arabidopsis Protoplasts”, Max Planck International Research School,basic lecture, Max Planck Institute for Chemical Ecology, Jena, Feb. 6-8, 2012
3. “Advanced Molecular Cloning and Application of Arabidopsis Protoplasts”, Max Planck International Research School, basic lecture, Max Planck Institute for Chemical Ecology,Jena, November 7-11, 2011
4. “Application of quantitative real-time PCR in ecological studies”, Ecology Workshop, Friedrich Schiller University, Jena, July, 2010
5. “Basic Knowledge of Molecular Cloning”, Max Planck International Research School, basic lecture, Max Planck Institute for Chemical Ecology, Jena, June, 2009
6. “Molecular Cloning of PCR Products”, Ecology Workshop, Friedrich Schiller University,Jena, July, 2008

Jun. 2008, Otto Hahn medal, Max Planck Society.

Review Articles
1. Hettenhausen, C., Schuman, M.C., Wu, J.* (2014) MAPK signaling – a key element in plant defense response to insects. Insect Science 22, 157-164. (IF=2.55)
http://www.ncbi.nlm.nih.gov/pubmed/24753304
2. Wang, L., Wu, J.* (2013) The essential role of jasmonic acid in plant-herbivore interactions - using the wild tobacco Nicotiana attenuata as a model. Journal of Genetics and Genomics 40, 597-606. (IF=3.98)
http://www.ncbi.nlm.nih.gov/pubmed/24377866
3. Heinrich, M., Baldwin, I.T., Wu, J.* (2012) Protein kinases in plant growth and defense. Protein kinases in plant growth and defense. Journal of Endocytobiosis and Cell Research 22, 48-51.
4. Hettenhausen, C., Baldwin, I.T., Wu, J.* (2012) MAPK signaling regulates multiple stress responses in plants. Journal of Endocytobiosis and Cell Research 22, 52-6.
5. Meldau, S., Baldwin, I.T., Wu, J.* (2011) For security and stability: SGT1 in plant defense and development. Plant Signaling & Behavior 6, 1479-82.
http://www.ncbi.nlm.nih.gov/pubmed/21897126
6. Yang D.H., Hettenhausen C., Baldwin, I.T., Wu, J.* (2011) The multifaceted function of BAK1/SERK3: plant immunity to pathogens and responses to insect herbivores. Plant Signaling & Behavior 6, 1322-4.
http://www.ncbi.nlm.nih.gov/pubmed/21852758
7. Wu, J.*, Baldwin, I.T.* (2010) New insights into plant responses to the attack from insect herbivores. Annual Review of Genetics 44, 1-24. (IF=12.23)
http://www.ncbi.nlm.nih.gov/pubmed/20649414
8. Wu, J., Baldwin, I.T.* (2009) Herbivory-induced signaling in plants: perception and action. Plant Cell & Environment 32, 1161-74. (IF=6.17)
http://www.ncbi.nlm.nih.gov/pubmed/19183291
Research Papers
Fist Author Papers
1. Wu, J., Hettenhausen, C., Schuman, M.C., and Baldwin, I.T.* (2008) A comparison of two Nicotiana attenuata accessions reveals large differences in Manduca sexta-induced signaling events. Plant Physiology 146, 927-39. (IF=6.28)
http://www.ncbi.nlm.nih.gov/pubmed/18218965
2. Wu, J., Hettenhausen, C., Meldau, S., and Baldwin, I.T.* (2007). Herbivory rapidly activates MAPK signaling in attacked and unattacked leaf regions but not between leaves of Nicotiana attenuata. Plant Cell 19, 1096-1122. (IF=8.54)
http://www.ncbi.nlm.nih.gov/pubmed/17400894
3. Wu, J., Kang, J.H., Hettenhausen, C., and Baldwin, I.T.* (2007). Nonsense-mediated mRNA decay (NMD) silences the accumulation of aberrant trypsin proteinase inhibitor mRNA in Nicotiana attenuata. Plant Journal 51, 693-706. (IF=5.47)
http://www.ncbi.nlm.nih.gov/pubmed/17587303
4. Wu, J., Hettenhausen, C., Baldwin, I.T.* (2006). Evolution of proteinase inhibitor defenses in North American  allopolyploid species of Nicotiana. Planta 224, 750-760.(IF=3.24)
http://www.ncbi.nlm.nih.gov/pubmed/16534618
Correspondence Author Papers
5. Sun, T., Renner, S., Xu, Y., Qin, Y., Wu, J.*, Sun, G.* (2016) Two hAT transposon genes were transferred from Brassicaceae to broomrapes and are actively expressed in some recipients. Scientific Reports (in press). (IF=5.23)
6. Luo, J., Wei, K., Wang, S., Zhao, W., Ma, C., Hettenhausen, C., Wu, J., Cao, G., Sun, G., Baldwin, I. T., Wu, J.*, Wang, L*. (2016) COI1-regulated hydroxylation of jasmonoyl-Lisoleucine impairs Nicotiana attenuata’s resistance to the generalist herbivore Spodoptera litura. Journal of Agricultural and Food Chemistry 64, 2822-2831
(IF=2.86)
http://www.ncbi.nlm.nih.gov/pubmed/26985773
7. Qi, J. #, Sun, G. #, Wang, L. #, Zhao, C. #, Hettenhausen, C., Schuman, M.C., Baldwin, I.T., Li, J., Song, J., Liu, Z., Xu, G., Lu, X., Wu, J.* (2016) Oral secretions from Mythimna separata insects specifically induce defense responses in maize as revealed by highdimensional biological data. Plant Cell & Environment (In press) (IF=6.17)
http://www.ncbi.nlm.nih.gov/pubmed/26991784
8. Hettenhausen, C#., Sun, G. #, He, Y., Zhuang, H., Sun, T., Qi, J., Wu, J.* (2016) Genome-wide identification of calcium-dependent protein kinases in soybean and analyses of their transcriptional responses to insect herbivory and drought stress.Scientific Reports, 6: 18973. (IF=5.23)
http://www.ncbi.nlm.nih.gov/pubmed/26733237
9. Li, J., Hettenhausen, C., Sun, G., Zhuang, H., Li, J. H.*, Wu, J.* (2015) The parasitic plant Cuscuta australis is highly insensitive to abscisic acid-induced suppression of hypocotyl elongation and seed germination. PLoS One, 10: e0135197. (IF=3.06)
http://www.ncbi.nlm.nih.gov/pubmed/26258814
10. Hettenhausen, C., Heinrich, M., Baldwin, I.T., Wu, J.* (2014) Fatty acid-amino acid conjugates are essential for systemic activation of salicylic acid-induced protein kinase and accumulation of jasmonic acid in Nicotiana attenuata. BMC Plant Biology, 14, 326. (IF=3.63)
http://www.ncbi.nlm.nih.gov/pubmed/25430398
11. Zhang, D., Qi, J., Yue, J., Huang, J., Sun, T., Li, S., Wen, J., Hettenhausen, C., Wu, J., Wang, L., Zhuang, H., Wu, J.* and Sun, G.* (2014), Root parasitic plant Orobanche aegyptiaca and shoot parasitic plant Cuscuta australis obtained Brassicaceae-specific strictosidine synthase-like genes by horizontal gene transfer. BMC Plant Biology 14, 19.
(IF=3.63)
http://www.ncbi.nlm.nih.gov/pubmed/24411025
12. Hettenhausen, C., Baldwin, I.T., Wu, J.* (2013) Nicotiana attenuata MPK4 suppresses a novel JA signaling-independent defense pathway against the specialist insect Manduca sexta but is not required for the resistance to the generalist Spodoptera littoralis. New Phytologist 199, 787-99. (IF=7.21)
http://www.ncbi.nlm.nih.gov/pubmed/23672856
13. Yang, D.H., Baldwin, I.T., Wu, J.* (2013) Silencing brassinosteroid receptor BRI1 impairs herbivory-elicited accumulation of jasmonic acid-isoleucine and diterpene glycosides, but not jasmonic acid and trypsin proteinase inhibitors in Nicotiana attenuata. Journal of Integrative Plant Biology 55, 514-526. (IF=3.67)
http://www.ncbi.nlm.nih.gov/pubmed/23347255
14. Heinrich, M., Hettenhausen, C., Lange, T., Wünsche, H., Fang, J., Baldwin, I.T., Wu, J.* (2013) High levels of jasmonic acid antagonize the biosynthesis of gibberellins and inhibit the growth of Nicotiana attenuata stems. Plant Journal 73, 591-606. (IF=5.47)
http://www.ncbi.nlm.nih.gov/pubmed/23190261
15. Hettenhausen, C., Yang, D.H., Baldwin, I.T., Wu, J.* (2012) Calcium-dependent protein kinases, CDPK4 and CDPK5, affect early steps of jasmonic acid biosynthesis in Nicotiana attenuata. Plant Signaling & Behavior 8, e22784
http://www.ncbi.nlm.nih.gov/pubmed/23221744
16. Yang, D.H., Hettenhausen, C., Baldwin, I.T., Wu, J.* (2012) Silencing Nicotiana attenuata calcium-dependent protein kinases, CDPK4 and CDPK5, strongly upregulates wound- and herbivory-induced jasmonic acid accumulations. Plant Physiology 159, 1591-607 (IF=6.28)
http://www.ncbi.nlm.nih.gov/pubmed/22715110
17. Hettenhausen, C., Baldwin, I.T., Wu, J.* (2012) Silencing MPK4 in Nicotiana attenuata enhances photosynthesis and seed production but compromises abscisic acid-induced stomatal closure and guard cell-mediated resistance to Pseudomonas syringae pv.tomato DC3000. Plant Physiology 158, 759-76 (IF=6.28)
http://www.ncbi.nlm.nih.gov/pubmed/22147519
18. Shi, C., Baldwin, I.T., Wu, J.* (2012) Arabidopsis nonsense-mediated mRNA decay factors, UPF1, UPF2, and UPF3, are involved in plant development and wounding- and pathogen-induced responses. Journal of Integrative Plant Biology 54, 99-114. (IF=3.67)
http://www.ncbi.nlm.nih.gov/pubmed/22353561
19. Heinrich, M., Baldwin, I.T., Wu, J.* (2012) Three MAPK kinases, MEK1, SIPKK and NPK2, are not involved in activation of SIPK after wounding and herbivore feeding but important for accumulation of trypsin proteinase inhibitors. Plant Molecular Biology Reporter 30, 731-40. (IF=2.30)
http://www.springerlink.com/content/ph4hq3w1318k5503/
20. Heinrich, M., Baldwin, I.T., Wu, J.* (2011) Two mitogen-activated protein kinase kinases, MKK1 and MEK2, are involved in wounding- and specialist lepidopteran herbivore Manduca sexta-induced responses in Nicotiana attenuata. Journal of Experimental Botany 62, 4355-65. (IF=5.68)
http://www.ncbi.nlm.nih.gov/pubmed/21610019
21. Wünsche, H., Baldwin, I.T., Wu, J.* (2011) S-Nitrosoglutathione reductase (GSNOR) mediates resistance of Nicotiana attenuata to the specialist insect herbivore Manduca sexta. Journal of Experimental Botany 62, 4605-16. (IF=5.68)
http://www.ncbi.nlm.nih.gov/pubmed/21622839
22. Wünsche, H., Baldwin, I.T., Wu, J.* (2011) Silencing NOA1 elevates herbivory-induced JA accumulation and compromises most of carbon-based defense metabolites in Nicotiana attenuata. Journal of Integrative Plant Biology 53, 619-31. (IF=3.67)
http://www.ncbi.nlm.nih.gov/pubmed/21457460
23. Yang, D.H., Hettenhausen, C., Baldwin, I.T., Wu, J.* (2011) BAK1 regulates the accumulation of jasmonic acid and the levels of trypsin proteinase inhibitors in Nicotiana attenuata’s responses to herbivory. Journal of Experimental Botany 62, 641-52. (IF=5.68)
http://www.ncbi.nlm.nih.gov/pubmed/20937731
24. Meldau, S., Baldwin, I.T., Wu, J.* (2011) SGT1 regulates wounding- and herbivoryinduced jasmonic acid accumulation and Nicotiana attenuata’s resistance to the specialist lepidopteran herbivore Manduca sexta. New Phytologist 189, 1143-56.(IF=7.21)
http://www.ncbi.nlm.nih.gov/pubmed/21118264
Other Papers
25. Sun, H., Wang, L., Zhang, B., Ma, J., Hettenhausen, C., Cao, G., Sun, G., Wu, J., Wu, J*. (2014) Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling. Journal of Experimental Botany 65, 4305-15. (IF=5.68)
http://www.ncbi.nlm.nih.gov/pubmed/24821958
26. Zhang, N., Han Z., Sun, G., Hoffman, A., Wilson, I.W., Yang, Y., Gao, Q., Wu, J., Xie, D., Dai, J., Qiu, D. (2014) Molecular cloning and characterization of a cytochrome P450 taxoid 9alpha-hydroxylase in Ginkgo biloba cells. Biochemical and Biophysical Research Communications 443, 938-43. (IF=2.37)
http://www.ncbi.nlm.nih.gov/pubmed/24380857
27. Sun, H., Hu, X., Ma, C., Hettenhausen, C., Wang, L., Sun, G., Wu, J., Wu, J*. (2013) Requirement of ABA signalling-mediated stomatal closure for resistance of wild tobacco to Alternaria alternate. Plant Pathology 63, 1070-7. (IF=2.38)
http://onlinelibrary.wiley.com/doi/10.1111/ppa.12181/abstract
28. Sun, G., Yang, Y., Xie, F., Wen, J.F., Wu J., Wilson, I.W., Tang, Q., Liu, H., Qiu, D. (2013) Deep sequencing reveals transcriptome re-programming of Taxus × media cells to the elicitation with methyl jasmonate. PLoS One 8, e62865. (IF=3.06)
http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062865
29. Yu, S., Cao, L., Zhou, C.M., Zhang, T.Q., Lian, H., Sun, Y., Wu, J., Wang, G., Wang, J.W., (2013) Sugar is an endogenous cue for juvenile-to-adult phase transition in plants. eLife 2, e00269. (IF=8.30)
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3610343/
30. Deng, W.W., Zhang, M., Wu J., Li, Y.Y., Wei, C.L.*, Jiang, C.J., Wan, X.C. (2013) Molecular cloning, functional analysis of three cinnamyl alcohol dehydrogenase (CAD) genes in the leaves of tea plant, Camellia sinensis. Journal of Plant Physiology 170, 272-282 (IF=2.97)
http://www.ncbi.nlm.nih.gov/pubmed/23228629
31. Meldau, S., Wu, J., Baldwin, I.T.* (2009) Silencing two herbivory-activated MAP kinases, SIPK and WIPK, does not increase Nicotiana attenuata’s susceptibility to herbivores in the glasshouse and in nature. New Phytologist 181, 161-73. (IF=7.21)
http://www.ncbi.nlm.nih.gov/pubmed/19076722
32. Rayapuram, C., Wu, J., Hase, C., and Baldwin, I.T.* (2008) PR-13/Thionin not PR-1 mediates bacterial resistance in Nicotiana attenuata in nature and neither influences herbivore resistance. Molecular Plant-Microbe Interactions 21, 988-1000. (IF=4.15)
http://www.ncbi.nlm.nih.gov/pubmed/18533839
33. Horn, M., Patankar, A.G., Zavala, J.A., Wu, J., Doleckova-Maresova, L., Vujtechova, M., Mares, M., Baldwin, I.T.* (2005). Differential elicitation of two processing proteases controls the processing pattern of the trypsin proteinase inhibitor precursor in Nicotiana attenuata. Plant Physiology 139, 375-388. (IF=6.28)
http://www.ncbi.nlm.nih.gov/pubmed/16113221
Book Chapters
1. Hettenhausen C, Baldwin, I.T., Wu J. (2014) Virus-induced gene silencing in plant MAPK research. Methods in Molecular Biology – Plant MAP Kinases: Methods and Protocols. Eds. G. Komis, J. Samaj, Humana Press Inc. 1171:79-89
http://www.ncbi.nlm.nih.gov/pubmed/24908121
2. Galis I., Schuman M.C., Gase K., Hettenhausen C., Hartl M., Dinh S.T., Wu J., Bonaventure G., Baldwin I.T. (2013) The use of VIGS technology to study plant -herbivore interactions. Methods in Molecular Biology - Virus-induced gene silencing:Methods and protocols. Eds. A. Becker, Humana Press Inc. 975:109-37
http://www.ncbi.nlm.nih.gov/pubmed/23386299
3. Tretyakov, A., Mrotzek, G., Wu, J., Baldwin, I.T., Saluz, H.P.* (2006). Rapid heatblock thermocycling of small samples: a path to fast, low-cost plant genotyping. Floriculture, Ornamental and Plant Biotechnology. Vol. 4. Global Science Books, Isleworth, 226- 230.

Review Articles
1. Hettenhausen, C., Schuman, M.C., Wu, J.* (2014) MAPK signaling – a key element in plant defense response to insects. Insect Science 22, 157-164. (IF=2.55)
http://www.ncbi.nlm.nih.gov/pubmed/24753304
2. Wang, L., Wu, J.* (2013) The essential role of jasmonic acid in plant-herbivore interactions - using the wild tobacco Nicotiana attenuata as a model. Journal of Genetics and Genomics 40, 597-606. (IF=3.98)
http://www.ncbi.nlm.nih.gov/pubmed/24377866
3. Heinrich, M., Baldwin, I.T., Wu, J.* (2012) Protein kinases in plant growth and defense. Protein kinases in plant growth and defense. Journal of Endocytobiosis and Cell Research 22, 48-51.
4. Hettenhausen, C., Baldwin, I.T., Wu, J.* (2012) MAPK signaling regulates multiple stress responses in plants. Journal of Endocytobiosis and Cell Research 22, 52-6.
5. Meldau, S., Baldwin, I.T., Wu, J.* (2011) For security and stability: SGT1 in plant defense and development. Plant Signaling & Behavior 6, 1479-82.
http://www.ncbi.nlm.nih.gov/pubmed/21897126
6. Yang D.H., Hettenhausen C., Baldwin, I.T., Wu, J.* (2011) The multifaceted function of BAK1/SERK3: plant immunity to pathogens and responses to insect herbivores. Plant Signaling & Behavior 6, 1322-4.
http://www.ncbi.nlm.nih.gov/pubmed/21852758
7. Wu, J.*, Baldwin, I.T.* (2010) New insights into plant responses to the attack from insect herbivores. Annual Review of Genetics 44, 1-24. (IF=12.23)
http://www.ncbi.nlm.nih.gov/pubmed/20649414
8. Wu, J., Baldwin, I.T.* (2009) Herbivory-induced signaling in plants: perception and action. Plant Cell & Environment 32, 1161-74. (IF=6.17)
http://www.ncbi.nlm.nih.gov/pubmed/19183291
Research Papers
Fist Author Papers
1. Wu, J., Hettenhausen, C., Schuman, M.C., and Baldwin, I.T.* (2008) A comparison of two Nicotiana attenuata accessions reveals large differences in Manduca sexta-induced signaling events. Plant Physiology 146, 927-39. (IF=6.28)
http://www.ncbi.nlm.nih.gov/pubmed/18218965
2. Wu, J., Hettenhausen, C., Meldau, S., and Baldwin, I.T.* (2007). Herbivory rapidly activates MAPK signaling in attacked and unattacked leaf regions but not between leaves of Nicotiana attenuata. Plant Cell 19, 1096-1122. (IF=8.54)
http://www.ncbi.nlm.nih.gov/pubmed/17400894
3. Wu, J., Kang, J.H., Hettenhausen, C., and Baldwin, I.T.* (2007). Nonsense-mediated mRNA decay (NMD) silences the accumulation of aberrant trypsin proteinase inhibitor mRNA in Nicotiana attenuata. Plant Journal 51, 693-706. (IF=5.47)
http://www.ncbi.nlm.nih.gov/pubmed/17587303
4. Wu, J., Hettenhausen, C., Baldwin, I.T.* (2006). Evolution of proteinase inhibitor defenses in North American  allopolyploid species of Nicotiana. Planta 224, 750-760.(IF=3.24)
http://www.ncbi.nlm.nih.gov/pubmed/16534618
Correspondence Author Papers
5. Sun, T., Renner, S., Xu, Y., Qin, Y., Wu, J.*, Sun, G.* (2016) Two hAT transposon genes were transferred from Brassicaceae to broomrapes and are actively expressed in some recipients. Scientific Reports (in press). (IF=5.23)
6. Luo, J., Wei, K., Wang, S., Zhao, W., Ma, C., Hettenhausen, C., Wu, J., Cao, G., Sun, G., Baldwin, I. T., Wu, J.*, Wang, L*. (2016) COI1-regulated hydroxylation of jasmonoyl-Lisoleucine impairs Nicotiana attenuata’s resistance to the generalist herbivore Spodoptera litura. Journal of Agricultural and Food Chemistry 64, 2822-2831
(IF=2.86)
http://www.ncbi.nlm.nih.gov/pubmed/26985773
7. Qi, J. #, Sun, G. #, Wang, L. #, Zhao, C. #, Hettenhausen, C., Schuman, M.C., Baldwin, I.T., Li, J., Song, J., Liu, Z., Xu, G., Lu, X., Wu, J.* (2016) Oral secretions from Mythimna separata insects specifically induce defense responses in maize as revealed by highdimensional biological data. Plant Cell & Environment (In press) (IF=6.17)
http://www.ncbi.nlm.nih.gov/pubmed/26991784
8. Hettenhausen, C#., Sun, G. #, He, Y., Zhuang, H., Sun, T., Qi, J., Wu, J.* (2016) Genome-wide identification of calcium-dependent protein kinases in soybean and analyses of their transcriptional responses to insect herbivory and drought stress.Scientific Reports, 6: 18973. (IF=5.23)
http://www.ncbi.nlm.nih.gov/pubmed/26733237
9. Li, J., Hettenhausen, C., Sun, G., Zhuang, H., Li, J. H.*, Wu, J.* (2015) The parasitic plant Cuscuta australis is highly insensitive to abscisic acid-induced suppression of hypocotyl elongation and seed germination. PLoS One, 10: e0135197. (IF=3.06)
http://www.ncbi.nlm.nih.gov/pubmed/26258814
10. Hettenhausen, C., Heinrich, M., Baldwin, I.T., Wu, J.* (2014) Fatty acid-amino acid conjugates are essential for systemic activation of salicylic acid-induced protein kinase and accumulation of jasmonic acid in Nicotiana attenuata. BMC Plant Biology, 14, 326. (IF=3.63)
http://www.ncbi.nlm.nih.gov/pubmed/25430398
11. Zhang, D., Qi, J., Yue, J., Huang, J., Sun, T., Li, S., Wen, J., Hettenhausen, C., Wu, J., Wang, L., Zhuang, H., Wu, J.* and Sun, G.* (2014), Root parasitic plant Orobanche aegyptiaca and shoot parasitic plant Cuscuta australis obtained Brassicaceae-specific strictosidine synthase-like genes by horizontal gene transfer. BMC Plant Biology 14, 19.
(IF=3.63)
http://www.ncbi.nlm.nih.gov/pubmed/24411025
12. Hettenhausen, C., Baldwin, I.T., Wu, J.* (2013) Nicotiana attenuata MPK4 suppresses a novel JA signaling-independent defense pathway against the specialist insect Manduca sexta but is not required for the resistance to the generalist Spodoptera littoralis. New Phytologist 199, 787-99. (IF=7.21)
http://www.ncbi.nlm.nih.gov/pubmed/23672856
13. Yang, D.H., Baldwin, I.T., Wu, J.* (2013) Silencing brassinosteroid receptor BRI1 impairs herbivory-elicited accumulation of jasmonic acid-isoleucine and diterpene glycosides, but not jasmonic acid and trypsin proteinase inhibitors in Nicotiana attenuata. Journal of Integrative Plant Biology 55, 514-526. (IF=3.67)
http://www.ncbi.nlm.nih.gov/pubmed/23347255
14. Heinrich, M., Hettenhausen, C., Lange, T., Wünsche, H., Fang, J., Baldwin, I.T., Wu, J.* (2013) High levels of jasmonic acid antagonize the biosynthesis of gibberellins and inhibit the growth of Nicotiana attenuata stems. Plant Journal 73, 591-606. (IF=5.47)
http://www.ncbi.nlm.nih.gov/pubmed/23190261
15. Hettenhausen, C., Yang, D.H., Baldwin, I.T., Wu, J.* (2012) Calcium-dependent protein kinases, CDPK4 and CDPK5, affect early steps of jasmonic acid biosynthesis in Nicotiana attenuata. Plant Signaling & Behavior 8, e22784
http://www.ncbi.nlm.nih.gov/pubmed/23221744
16. Yang, D.H., Hettenhausen, C., Baldwin, I.T., Wu, J.* (2012) Silencing Nicotiana attenuata calcium-dependent protein kinases, CDPK4 and CDPK5, strongly upregulates wound- and herbivory-induced jasmonic acid accumulations. Plant Physiology 159, 1591-607 (IF=6.28)
http://www.ncbi.nlm.nih.gov/pubmed/22715110
17. Hettenhausen, C., Baldwin, I.T., Wu, J.* (2012) Silencing MPK4 in Nicotiana attenuata enhances photosynthesis and seed production but compromises abscisic acid-induced stomatal closure and guard cell-mediated resistance to Pseudomonas syringae pv.tomato DC3000. Plant Physiology 158, 759-76 (IF=6.28)
http://www.ncbi.nlm.nih.gov/pubmed/22147519
18. Shi, C., Baldwin, I.T., Wu, J.* (2012) Arabidopsis nonsense-mediated mRNA decay factors, UPF1, UPF2, and UPF3, are involved in plant development and wounding- and pathogen-induced responses. Journal of Integrative Plant Biology 54, 99-114. (IF=3.67)
http://www.ncbi.nlm.nih.gov/pubmed/22353561
19. Heinrich, M., Baldwin, I.T., Wu, J.* (2012) Three MAPK kinases, MEK1, SIPKK and NPK2, are not involved in activation of SIPK after wounding and herbivore feeding but important for accumulation of trypsin proteinase inhibitors. Plant Molecular Biology Reporter 30, 731-40. (IF=2.30)
http://www.springerlink.com/content/ph4hq3w1318k5503/
20. Heinrich, M., Baldwin, I.T., Wu, J.* (2011) Two mitogen-activated protein kinase kinases, MKK1 and MEK2, are involved in wounding- and specialist lepidopteran herbivore Manduca sexta-induced responses in Nicotiana attenuata. Journal of Experimental Botany 62, 4355-65. (IF=5.68)
http://www.ncbi.nlm.nih.gov/pubmed/21610019
21. Wünsche, H., Baldwin, I.T., Wu, J.* (2011) S-Nitrosoglutathione reductase (GSNOR) mediates resistance of Nicotiana attenuata to the specialist insect herbivore Manduca sexta. Journal of Experimental Botany 62, 4605-16. (IF=5.68)
http://www.ncbi.nlm.nih.gov/pubmed/21622839
22. Wünsche, H., Baldwin, I.T., Wu, J.* (2011) Silencing NOA1 elevates herbivory-induced JA accumulation and compromises most of carbon-based defense metabolites in Nicotiana attenuata. Journal of Integrative Plant Biology 53, 619-31. (IF=3.67)
http://www.ncbi.nlm.nih.gov/pubmed/21457460
23. Yang, D.H., Hettenhausen, C., Baldwin, I.T., Wu, J.* (2011) BAK1 regulates the accumulation of jasmonic acid and the levels of trypsin proteinase inhibitors in Nicotiana attenuata’s responses to herbivory. Journal of Experimental Botany 62, 641-52. (IF=5.68)
http://www.ncbi.nlm.nih.gov/pubmed/20937731
24. Meldau, S., Baldwin, I.T., Wu, J.* (2011) SGT1 regulates wounding- and herbivoryinduced jasmonic acid accumulation and Nicotiana attenuata’s resistance to the specialist lepidopteran herbivore Manduca sexta. New Phytologist 189, 1143-56.(IF=7.21)
http://www.ncbi.nlm.nih.gov/pubmed/21118264
Other Papers
25. Sun, H., Wang, L., Zhang, B., Ma, J., Hettenhausen, C., Cao, G., Sun, G., Wu, J., Wu, J*. (2014) Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling. Journal of Experimental Botany 65, 4305-15. (IF=5.68)
http://www.ncbi.nlm.nih.gov/pubmed/24821958
26. Zhang, N., Han Z., Sun, G., Hoffman, A., Wilson, I.W., Yang, Y., Gao, Q., Wu, J., Xie, D., Dai, J., Qiu, D. (2014) Molecular cloning and characterization of a cytochrome P450 taxoid 9alpha-hydroxylase in Ginkgo biloba cells. Biochemical and Biophysical Research Communications 443, 938-43. (IF=2.37)
http://www.ncbi.nlm.nih.gov/pubmed/24380857
27. Sun, H., Hu, X., Ma, C., Hettenhausen, C., Wang, L., Sun, G., Wu, J., Wu, J*. (2013) Requirement of ABA signalling-mediated stomatal closure for resistance of wild tobacco to Alternaria alternate. Plant Pathology 63, 1070-7. (IF=2.38)
http://onlinelibrary.wiley.com/doi/10.1111/ppa.12181/abstract
28. Sun, G., Yang, Y., Xie, F., Wen, J.F., Wu J., Wilson, I.W., Tang, Q., Liu, H., Qiu, D. (2013) Deep sequencing reveals transcriptome re-programming of Taxus × media cells to the elicitation with methyl jasmonate. PLoS One 8, e62865. (IF=3.06)
http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062865
29. Yu, S., Cao, L., Zhou, C.M., Zhang, T.Q., Lian, H., Sun, Y., Wu, J., Wang, G., Wang, J.W., (2013) Sugar is an endogenous cue for juvenile-to-adult phase transition in plants. eLife 2, e00269. (IF=8.30)
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3610343/
30. Deng, W.W., Zhang, M., Wu J., Li, Y.Y., Wei, C.L.*, Jiang, C.J., Wan, X.C. (2013) Molecular cloning, functional analysis of three cinnamyl alcohol dehydrogenase (CAD) genes in the leaves of tea plant, Camellia sinensis. Journal of Plant Physiology 170, 272-282 (IF=2.97)
http://www.ncbi.nlm.nih.gov/pubmed/23228629
31. Meldau, S., Wu, J., Baldwin, I.T.* (2009) Silencing two herbivory-activated MAP kinases, SIPK and WIPK, does not increase Nicotiana attenuata’s susceptibility to herbivores in the glasshouse and in nature. New Phytologist 181, 161-73. (IF=7.21)
http://www.ncbi.nlm.nih.gov/pubmed/19076722
32. Rayapuram, C., Wu, J., Hase, C., and Baldwin, I.T.* (2008) PR-13/Thionin not PR-1 mediates bacterial resistance in Nicotiana attenuata in nature and neither influences herbivore resistance. Molecular Plant-Microbe Interactions 21, 988-1000. (IF=4.15)
http://www.ncbi.nlm.nih.gov/pubmed/18533839
33. Horn, M., Patankar, A.G., Zavala, J.A., Wu, J., Doleckova-Maresova, L., Vujtechova, M., Mares, M., Baldwin, I.T.* (2005). Differential elicitation of two processing proteases controls the processing pattern of the trypsin proteinase inhibitor precursor in Nicotiana attenuata. Plant Physiology 139, 375-388. (IF=6.28)
http://www.ncbi.nlm.nih.gov/pubmed/16113221
Book Chapters
1. Hettenhausen C, Baldwin, I.T., Wu J. (2014) Virus-induced gene silencing in plant MAPK research. Methods in Molecular Biology – Plant MAP Kinases: Methods and Protocols. Eds. G. Komis, J. Samaj, Humana Press Inc. 1171:79-89
http://www.ncbi.nlm.nih.gov/pubmed/24908121
2. Galis I., Schuman M.C., Gase K., Hettenhausen C., Hartl M., Dinh S.T., Wu J., Bonaventure G., Baldwin I.T. (2013) The use of VIGS technology to study plant -herbivore interactions. Methods in Molecular Biology - Virus-induced gene silencing:Methods and protocols. Eds. A. Becker, Humana Press Inc. 975:109-37
http://www.ncbi.nlm.nih.gov/pubmed/23386299
3. Tretyakov, A., Mrotzek, G., Wu, J., Baldwin, I.T., Saluz, H.P.* (2006). Rapid heatblock thermocycling of small samples: a path to fast, low-cost plant genotyping. Floriculture, Ornamental and Plant Biotechnology. Vol. 4. Global Science Books, Isleworth, 226- 230.

1.Plants have co-evolved with insect herbivores for hundreds of millions of years and have elaborate defense systems against the attack from herbivores. Moreover, insects also cause large economical loss each year in crop production and environmental-friendly pest control is one of the important aspects of modern agriculture.

Using several model plant species, such as soybean (Glycine max), wild tobacco Nicotianaattenuata, cultivated tobacco N. tabacum, and Arabidopsis thaliana, and chewing and sucking insects, we intend to answer the following research questions:

1)How do plants sense the attack of insects;

2)Which genes are involved in herbivory-induced signal transduction, and how do they execute their functions?

3)Which plant secondary metabolites are induced by herbivore feeding and have anti-herbivore functions? Which genes encode their biosynthetic enzymes?

Research objective: To understand the molecular mechanisms by which plants modulate their resistance to herbivores, and to develop novel insect control strategies using non-toxic plant-derived chemicals and genetically engineering or breeding.

2.One percent of the angiosperm plants are parasitic. Compared with other autotrophic plants, heterotrophic parasitic plants have interesting distinct physiology, ecology, and evolutionary history. Many parasitic plants infect crop plants and this leads to decreased or even complete loss of yield. These parasites are also hard to control due to their intrinsic connection with the hosts.

Using dodders (Cuscuta spp.), which are holoparasitc plants, as the model, we are interested in:

1)Elucidating the mechanism of dodder foraging;

2)Identifying the important dodder-specific genes which control the development, especially those mediate the development of haustoria;

3)The molecular mechanisms of dodder transport of host nutrients, secondary metabolites and macromolecules;

4)Identifying genes that are important for host defense against parasitization.

Research objective: To understand the physiology, ecology, and evolutionary history of dodder and develop dodder bio-control methods.

1. National Congress of Plant Biology, Changchun, China, Oct. 9-12, 2015
2. International Symposium on “From Ecosystems to Modern Agriculture”, Lanzhou,China, June 26－27, 2015
3. 13th Congress on Parasitic Plants, Kunming, China, 5-10 July 2015
4. 3rd International Conference on Plant Metabolism, Xiamen, China, July 2-5, 2014
5. 10th Solanaceae Conference (SOL 2013), Beijing, China, Oct. 13-17, 2013
6. The important roles of MAPKs in plant defense against herbivores. Invited talk, Institute of Zoology, Chinese Academy of Sciences, Beijing, China, Jun 4, 2013
7. Herbivory-induced signaling in plants – MAPKs go ahead. Invited talk, Institute of Botany, Chinese Academy of Sciences, Beijing, China, Nov. 12, 2012
8. Herbivory-induced signaling in plants – MAPKs go ahead. Invited talk, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China, Nov. 14, 2012
9. MPK4 in stress signaling. Invited talk, Huazhong Agricultural University, Wuhan, China, Jul. 18, 2012
10. Functions of MAPK signaling in plant resistance to herbivores. Invited lecture, Chinese Academy of Forestry, Beijing, China, Dec. 27, 2011
11. MPK4 in Nicotiana attenuata: a multifaceted MAPK involved in biotic and abiotic resistance. 2nd International Symposium on Integrative Plant Biology, Invited lecture, Lanzhou, China, Aug. 26-28, 2011
12. Herbivory-Induced Signaling in Plants: Perception and Action. Invited lecture, Anhui Agricultural University, Feb 21, 2011

13. When an herbivore takes a bite, does the plant know? Invited plenary lecture, International Conference on Plant Vascular Biology and Agriculture, Chongqing, China, June 21-24, 2009
14. NaCDPK1 mediates heat resistance in Nicotiana attenuata. Max Planck Institute for Chemical Ecology, Jena, Germany, Sept. 25-26, 2008
15. MAP kinases regulate Nicotiana attenuata's defense responses to herbivory. Department of Life Sciences, Nanjing University, Nanjing, China, June 6, 2008
16. Genetic modifications of Nicotiana attenuata reveal functions of plant secondary metabolites in resistance to herbivory; Invited plenary lecture, International Conference on Plant Secondary Metabolism, Kunming, China, June 8-10, 2008
17. MAP kinases regulate defense responses to herbivory in Nicotiana attenuata; Max Planck Institute for Chemical Ecology, Jena, Germany, Sept. 2007
18. The evolution of proteinase inhibitor defense mechanisms during polyploidy speciation in Nicotiana native to North America, Workshop DFG-SPP 1152 “Evolution of metabolic diversity”, Halle, Germany, Oct. 2004
19. The evolution of herbivory-specific expression of proteinase inhibitors during polyploidy speciation in Nicotiana native to North America; Botanikertagung 2004/Deutsche Botanische Gesellschaft, Vereinigung für Angewandte Botanik, Braunschweig, Germany, Sept. 2004
 

Partner Group of the Max Planck Institute for Chemical Ecology

Dalian Institute of Chemical Physics, Chinese Academy of Sciences

Zhejiang University

Yunnan Agriculture University

Anhui Agricultural University

已指导学生

庄会富  博士研究生  071010-生物化学与分子生物学  

宋娟  博士研究生  071010-生物化学与分子生物学  

路承凯  博士研究生  071010-生物化学与分子生物学  

张翠萍  博士研究生  071010-生物化学与分子生物学  

李莎兰  博士研究生  071010-生物化学与分子生物学  

张井雄  博士研究生  071010-生物化学与分子生物学  

穆梦花  硕士研究生  085238-生物工程  

刘念  博士研究生  071010-生物化学与分子生物学  

卞金鸽  硕士研究生  071010-生物化学与分子生物学  

许宇星  博士研究生  071010-生物化学与分子生物学  

陈思霖  博士研究生  071010-生物化学与分子生物学  

周士钊  硕士研究生  071010-生物化学与分子生物学  

现指导学生

薛娜  博士研究生  071010-生物化学与分子生物学  

马灿容  博士研究生  071010-生物化学与分子生物学  

粟忠祥  硕士研究生  085238-生物工程  

杨建翔  博士研究生  071010-生物化学与分子生物学  

展澈  博士研究生  071010-生物化学与分子生物学  

赵漫  博士研究生  071010-生物化学与分子生物学  

郑茜杰  博士研究生  071010-生物化学与分子生物学  

李文兴  硕士研究生  071010-生物化学与分子生物学  

冯泽瑞  博士研究生  071010-生物化学与分子生物学  

张书涵  硕士研究生  071010-生物化学与分子生物学  

张立坚  博士研究生  071010-生物化学与分子生物学  

郑天胤  博士研究生  071010-生物化学与分子生物学  

郑维  博士研究生  071010-生物化学与分子生物学  

荣德庆  博士研究生  071010-生物化学与分子生物学