General
Zhenfeng Liu, Ph.D, Prof.
Principal Investigator
National Laboratory of Biomacromolecules, IBP
Structure and function of membrane proteins
E-mail：liuzf@sun5.ibp.ac.cn
Fax：010-64881481
Zip code：100101
Principal Investigator
National Laboratory of Biomacromolecules, IBP
Structure and function of membrane proteins
E-mail：liuzf@sun5.ibp.ac.cn
Fax：010-64881481
Zip code：100101
Research Areas
Membrane protein structural biology.
Education
1998 - 2004 Doctor of Science in Biophysics, August, 2004, Instiute of Biophysics, Chinese Academy of Sciences Beijing, China
1994 - 1998 Bachelor of Science in Biology, July, 1998, Department of Biology, Xiamen University, Fujian Province, China
1994 - 1998 Bachelor of Science in Biology, July, 1998, Department of Biology, Xiamen University, Fujian Province, China
Experience
Work Experience
Dec. 2004 - Dec. 2010 Research Associate, Howard Hughes Medical Institute, Postdoctoral Scholar Division of Chemistry and Chemical Engineering California Institute of Technology, Pasadena, California, USA
Jan. 2011 - present Investigator, Institute of Biophysics, Chinese Academy of Sciences, Beijing
Jan. 2011 - present Investigator, Institute of Biophysics, Chinese Academy of Sciences, Beijing
Honors & Distinctions
Nov. 2011- the Young Thousand Talents Program , Class A.
Beijing Science and Technology Award.
State Natural Science Award.
Beijing Science and Technology Award.
State Natural Science Award.
Publications
Papers
1. Li J., Guo J. L., Ou X. M., Zhang M. F., Li Y.Z. and Liu Z. F.* (2015). Mechanical coupling of the multiple structural elements of the large-conductance mechanosensitive channel during expansion. Proc. Natl. Acad. Sci. USA, doi/10.1073/pnas.1503202112.
2. Wei X. P., Guo J. T., Li M.and Liu Z. F.* (2015). Structural Mechanism Underlying the Specific Recognition between the Arabidopsis State-Transition Phosphatase TAP38/PPH1 and Phosphorylated Light-Harvesting Complex Protein Lhcb1. Plant Cell, 27: 1113-1127. (2015, doi: 10.1105/tpc.15.00102).
3. Liu X. Y., Yan Y., Wu J. J. and Liu Z. F.* (2014). Structure and mechanism of an intramembrane liponucleotide synthetase central for phospholipid biosynthesis. Nat. Commun. 5:4244.
4. Pan X. W., Liu Z. F.*, Li M. & Chang W. R.*. (2013) Architecture and function of plant light-harvesting complexes II. Curr. Opin. Struct. Biol. 23:515-525. (* Corresponding authors)
5. Guo J. T., Wei X. P., Li M., Pan X. W., Chang W. R.* & Liu Z. F.* (2013) Structure of the catalytic domain of a state transition kinase homolog from Micromonas algae. Protein & Cell 4, 607-619. (* Corresponding authors)
6. Liu Z.F.，Walton T.A.，Rees D.C. (2010) A reported archaeal mechanosensitive channel is a structural homolog of MarR-like transcriptional regulators. Protein Science 19，808-814.
7. Liu Z.F.，Gandhi C.S.，Rees D.C. (2009) Structure of a tetrameric MscL in an expanded intermediate state. Nature 461，120-124.
8. Liu Z.F.，Chang W.R. (2008) Structure of the light-harvesting complex II. In: Photosynthetic Protein Complexes. Wiley-VCH (Book chapter)Fromme P. ed., 217-242.
9. Liu Z.F.，Chang W.R. (2008) Crystallization Methods of Membrane Proteins: Practical Aspects of Crystallizing Plant Light-Harvesting Complexes. In: Biophysical Techniques in Photosynthesis II. Spinger (Book chapter)Aartsma T. J. and Matysik J. eds. 26，77-96.
10. Yan H.C.，Zhang P.F.，Wang C.，Liu Z.F.，Chang W.R. (2007) Two lutein molecules in LHCII have different conformations and functions: Insights into the molecular mechanism of thermal dissipation in plants. Biochem. Biophys. Res. Commun. 355，457-463.
11. Pascal A.A.，Liu Z.F.，Broess K.，Oort B.V.，Amerongen H.V.，Wang C.，Horton P.，Robert B.，Chang W.R.，Ruban A. (2005) Molecular basis of photoprotection and control of photosynthetic light-harvesting. Nature 436，134-137.
12. Liu Z.F.，Yan H.C.，Wang K.B.，Kuang T.Y.，Zhang J.P.，Gui L.L.，An X.M.，Chang W.R. (2004) Crystal structure of spinach major light-harvesting complex at 2.72 ? resolution. Nature 428，287-292.
2. Wei X. P., Guo J. T., Li M.and Liu Z. F.* (2015). Structural Mechanism Underlying the Specific Recognition between the Arabidopsis State-Transition Phosphatase TAP38/PPH1 and Phosphorylated Light-Harvesting Complex Protein Lhcb1. Plant Cell, 27: 1113-1127. (2015, doi: 10.1105/tpc.15.00102).
3. Liu X. Y., Yan Y., Wu J. J. and Liu Z. F.* (2014). Structure and mechanism of an intramembrane liponucleotide synthetase central for phospholipid biosynthesis. Nat. Commun. 5:4244.
4. Pan X. W., Liu Z. F.*, Li M. & Chang W. R.*. (2013) Architecture and function of plant light-harvesting complexes II. Curr. Opin. Struct. Biol. 23:515-525. (* Corresponding authors)
5. Guo J. T., Wei X. P., Li M., Pan X. W., Chang W. R.* & Liu Z. F.* (2013) Structure of the catalytic domain of a state transition kinase homolog from Micromonas algae. Protein & Cell 4, 607-619. (* Corresponding authors)
6. Liu Z.F.，Walton T.A.，Rees D.C. (2010) A reported archaeal mechanosensitive channel is a structural homolog of MarR-like transcriptional regulators. Protein Science 19，808-814.
7. Liu Z.F.，Gandhi C.S.，Rees D.C. (2009) Structure of a tetrameric MscL in an expanded intermediate state. Nature 461，120-124.
8. Liu Z.F.，Chang W.R. (2008) Structure of the light-harvesting complex II. In: Photosynthetic Protein Complexes. Wiley-VCH (Book chapter)Fromme P. ed., 217-242.
9. Liu Z.F.，Chang W.R. (2008) Crystallization Methods of Membrane Proteins: Practical Aspects of Crystallizing Plant Light-Harvesting Complexes. In: Biophysical Techniques in Photosynthesis II. Spinger (Book chapter)Aartsma T. J. and Matysik J. eds. 26，77-96.
10. Yan H.C.，Zhang P.F.，Wang C.，Liu Z.F.，Chang W.R. (2007) Two lutein molecules in LHCII have different conformations and functions: Insights into the molecular mechanism of thermal dissipation in plants. Biochem. Biophys. Res. Commun. 355，457-463.
11. Pascal A.A.，Liu Z.F.，Broess K.，Oort B.V.，Amerongen H.V.，Wang C.，Horton P.，Robert B.，Chang W.R.，Ruban A. (2005) Molecular basis of photoprotection and control of photosynthetic light-harvesting. Nature 436，134-137.
12. Liu Z.F.，Yan H.C.，Wang K.B.，Kuang T.Y.，Zhang J.P.，Gui L.L.，An X.M.，Chang W.R. (2004) Crystal structure of spinach major light-harvesting complex at 2.72 ? resolution. Nature 428，287-292.
Research Interests
Structure and function of membrane proteins from different biological systems, using (but not limited to) the techniques of X-ray crystallography and Cryo-electron Microscopy. The targets include the bacterial mechanosensitive channels involved in osmotic pressure regulation, intramembrane enzymes catalyzing phospholipid biosynthesis and remodeling, transporters vital for homeostasis of intracellular Fe2+/Mn2+ and membrane protein complexes related to photosynthetic state transition. The goal is to elucidate the molecular mechanism of these fundamental biological processes through in-depth structural and functional analyses.
Conferences
1.‘Phospholipid biosynthesis: the classical biochemical pathways renewed with structural insights’, COLD SPRING HARBOR ASIA CONFERENCES, May 11, 2015
2.‘Structure of a bacterial large-conductance mechanosensitive channel ‘,International Symposium on Mechanobiology, November 4, 2011
3.‘Structure of a bacterial large-conductance mechanosensitive channel’,17th International Biophysics Congress(IUPAB), October 30, 2011
4.‘Crystal structure of MscL from Staphylococcus aureus and the structure-based gating mechanism ‘Gordon Research Conferences: Cell Osmoregulation and Mechanotransduction, July 1, 2009
5.‘Crystal structure of spinach major light-harvesting complex at 2.72 A resolution’, The 13th International Congress of Photosynthesis 2004, August 1, 2004
6.‘Crystallization and Phasing of LHC-II A novel type of membrane protein crystal’, The 10th International Conference on the Crystallization of Biological Macromolecules (ICCBM10), June 1, 2004
2.‘Structure of a bacterial large-conductance mechanosensitive channel ‘,International Symposium on Mechanobiology, November 4, 2011
3.‘Structure of a bacterial large-conductance mechanosensitive channel’,17th International Biophysics Congress(IUPAB), October 30, 2011
4.‘Crystal structure of MscL from Staphylococcus aureus and the structure-based gating mechanism ‘Gordon Research Conferences: Cell Osmoregulation and Mechanotransduction, July 1, 2009
5.‘Crystal structure of spinach major light-harvesting complex at 2.72 A resolution’, The 13th International Congress of Photosynthesis 2004, August 1, 2004
6.‘Crystallization and Phasing of LHC-II A novel type of membrane protein crystal’, The 10th International Conference on the Crystallization of Biological Macromolecules (ICCBM10), June 1, 2004
Students
已指导学生
柴景超 硕士研究生 071010-生物化学与分子生物学
尹妍 博士研究生 071011-生物物理学
魏雪鹏 博士研究生 071011-生物物理学
李婕 博士研究生 071011-生物物理学
杨涵婷 博士研究生 071011-生物物理学
胡苗会 博士研究生 071011-生物物理学
焦海展 博士研究生 071011-生物物理学
盛鑫 博士研究生 071011-生物物理学
刘秀颖 博士研究生 071011-生物物理学
现指导学生
厉玉梅 博士研究生 071011-生物物理学
宋丹枫 博士研究生 071011-生物物理学
杨博伟 博士研究生 071011-生物物理学
单建宇 硕士研究生 071010-生物化学与分子生物学
刘昊 博士研究生 071011-生物物理学
李安节 博士研究生 071011-生物物理学
王伊迪 博士研究生 071011-生物物理学
荣耀 博士研究生 071011-生物物理学