Regeneration of Blood and immune​ lineages from pluripotent stem cells for translational purpose.

2021.7-, Institute of Zoology, CAS, Principal Investigator

2012.03-2021.6,  Guangzhou Insititutes of Biomedicine and Health，CAS, Principal Investigator

2007.09-2012.02, Medical College of Wisconsin &Univeristy of Wisconsin-Madison, Research Associate(Post Doc), Assistant Scientist

1.     Lin, Y., Z. Xiao, F. Hu, X. Zheng, C. Zhang, Y. Wang, Y. Liu, D. Huang, Z. Wang, C. Xia, Q. Weng, L. Zhang, Y. Zhao, H. Qi, Y. Shen, Y. Chen, F. Zhang, J. Wu, P. Liu, J. Xu, L. Liu, Y. Zhu, J. Zhang, W. Qian, A. Liang, X. Zhu, T. Wang, M. Zhang and J. Wang (2025). "Engineered CRO-CD7 CAR-NK cells derived from pluripotent stem cells avoid fratricide and efficiently suppress human T-cell malignancies." J Hematol Oncol 18(1): 57.

2.     Zhang, Q., C. Xia, Q. Weng, L. Zhang, Y. Wang, Y. Liu, X. Zheng, Y. Lin, Y. Chen, Y. Shen, H. Qi, L. Liu, Y. Zhu, M. Zhang, D. Huang, F. Hu, M. Zhang, H. Zeng, J. Wang and T. Wang (2024). "Hypoimmunogenic CD19 CAR-NK cells derived from embryonic stem cells suppress the progression of human B-cell malignancies in xenograft animals." Front Immunol 15: 1504459.

3.     Liu, Y., M. Zhang, X. Shen, C. Xia, F. Hu, D. Huang, Q. Weng, Q. Zhang, L. Liu, Y. Zhu, L. Wang, J. Hao, M. Zhang, T. Wang and J. Wang (2024). "Mesothelin CAR-engineered NK cells derived from human embryonic stem cells suppress the progression of human ovarian cancer in animals." Cell Prolif: e13727.

4.     Wang, Y., J. Li, Z. Wang, Y. Liu, T. Wang, M. Zhang, C. Xia, F. Zhang, D. Huang, L. Zhang, Y. Zhao, L. Liu, Y. Zhu, H. Qi, X. Zhu, W. Qian, F. Hu and J. Wang (2024). "Comparison of seven CD19 CAR designs in engineering NK cells for enhancing anti-tumour activity." Cell Prolif: e13683.

5.     Peng, H., Y. Lin, F. Hu, C. Lv, B. Wu, Q. Weng, L. Liu, C. Xia, X. Liu, Y. Zhao, Q. Zhang, Y. Geng, M. Zhang and J. Wang (2023). "Prolonged generation of multi-lineage blood cells in wild-type animals from pluripotent stem cells." Stem Cell Reports 18(3): 720-735.

6.     Wu, B., Q. Zhang, P. Hong, L. Liu, H. Peng, C. Xia, T. Wang, Y. Wang, Q. Weng, X. Liu, Y. Geng, J. Wang and H. Wu (2023). "Antigen-specific TCR-T cells from Rag2 gene-deleted pluripotent stem cells impede solid tumour growth in a mouse model." Cell Prolif: e13389.

7.     Xiong, J., Y. Zhao, Y. Lin, L. Chen, Q. Weng, C. Shi, X. Liu, Y. Geng, L. Liu, J. Wang and M. Zhang (2022). "Identification and characterization of innate lymphoid cells generated from pluripotent stem cells." Cell Rep 41(5): 111569.

8.     Huang, D., J. Li, F. Hu, C. Xia, Q. Weng, T. Wang, H. Peng, B. Wu, H. Wu, J. Xiong, Y. Lin, Y. Wang, Q. Zhang, X. Liu, L. Liu, X. Zheng, Y. Geng, X. Du, X. Zhu, L. Wang, J. Hao and J. Wang (2022). "Lateral plate mesoderm cell-based organoid system for NK cell regeneration from human pluripotent stem cells." Cell Discov 8(1): 121.

9.     Huang, D., Q. Zhao, M. Zhang, Q. Weng, Q. Zhang, K. Wang, F. Dong, H. Cheng, F. Hu and J. Wang (2022). "Hoxb5 reprogrammes murine multipotent blood progenitors into haematopoietic stem cell-like cells." Cell Prolif 55(6): e13235.

10.  Yu, B., B. Wu, P. Hong, H. Peng, M. Zhang, Q. Zhang, L. Liu, X. Liu, Y. Geng, J. Wang and Y. Lan (2022). "Co-Expression of Runx1, Hoxa9, Hlf, and Hoxa7 Confers Multi-Lineage Potential on Hematopoietic Progenitors Derived From Pluripotent Stem Cells." Front Cell Dev Biol 10: 859769.

11.  Zhang, Q., B. Wu, Q. Weng, F. Hu, Y. Lin, C. Xia, H. Peng, Y. Wang, X. Liu, L. Liu, J. Xiong, Y. Geng, Y. Zhao, M. Zhang, J. Du and J. Wang (2022). "Regeneration of immunocompetent B lymphopoiesis from pluripotent stem cells guided by transcription factors." Cell Mol Immunol 19(4): 492-503.

12.  Wang, T., C. Xia, Q. Weng, K. Wang, Y. Dong, S. Hao, F. Dong, X. Liu, L. Liu, Y. Geng, Y. Guan, J. Du, T. Cheng, H. Cheng and J. Wang (2022). "Loss of Nupr1 promotes engraftment by tuning the quiescence threshold of hematopoietic stem cell repository via regulating p53-checkpoint pathway." Haematologica 107(1): 154-166.

13.  Zhou, P., C. Xia, T. Wang, Y. Dong, Q. Weng, X. Liu, Y. Geng, J. Wang and J. Du (2021). "Senescent bone marrow microenvironment promotes Nras-mutant leukemia." J Mol Cell Biol 13(1): 72-74.

14.  Lv, C., S. Chen, F. Hu, D. Huang, T. Wang, J. Du, J. Wang and H. Wu (2021). "Pluripotent stem cell-derived CD19-CAR iT cells effectively eradicate B-cell lymphoma in vivo." Cell Mol Immunol 18(3): 773-775.

15.  Xia, C., T. Wang, H. Cheng, Y. Dong, Q. Weng, G. Sun, P. Zhou, K. Wang, X. Liu, Y. Geng, S. Ma, S. Hao, L. Xu, Y. Guan, J. Du, X. Du, Y. Li, X. Zhu, Y. Shi, S. Xu, D. Wang, T. Cheng and J. Wang (2020). "Mesenchymal stem cells suppress leukemia via macrophage-mediated functional restoration of bone marrow microenvironment." Leukemia 34(9): 2375-2383.

16.  Guo, R., F. Hu, Q. Weng, C. Lv, H. Wu, L. Liu, Z. Li, Y. Zeng, Z. Bai, M. Zhang, Y. Liu, X. Liu, C. Xia, T. Wang, P. Zhou, K. Wang, Y. Dong, Y. Luo, X. Zhang, Y. Guan, Y. Geng, J. Du, Y. Li, Y. Lan, J. Chen, B. Liu and J. Wang (2020). "Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors." Cell Res 30(1): 21-33.

17.  Guo, R., H. Wu, J. Du and J. Wang (2020). "T cell regeneration: an update on progress and challenges." Blood Sci 2(1): 22-26.

18.  Hu, F., D. Huang, Y. Luo, P. Zhou, C. Lv, K. Wang, Q. Weng, X. Liu, Y. Guan, Y. Geng, J. Du, J. Chen, J. Wang and H. Wu (2020). "Hematopoietic lineage-converted T cells carrying tumor-associated antigen-recognizing TCRs effectively kill tumor cells." J Immunother Cancer 8(2).

19.  Wang, T., C. Lv, F. Hu, L. Liu and J. Wang (2020). "Two-step protocol for regeneration of immunocompetent T cells from mouse pluripotent stem cells." Blood Sci 2(3): 79-88.

20.  Dong, Y., K. Wang, Q. Weng, T. Wang, P. Zhou, X. Liu, Y. Geng, L. Liu, H. Wu, J. Wang and J. Du (2020). "NUP98-HOXA10hd fusion protein sustains multi-lineage haematopoiesis of lineage-committed progenitors in transplant setting." Cell Prolif 53(9): e12885.

21.  Zhang, M., Y. Dong, F. Hu, D. Yang, Q. Zhao, C. Lv, Y. Wang, C. Xia, Q. Weng, X. Liu, C. Li, P. Zhou, T. Wang, Y. Guan, R. Guo, L. Liu, Y. Geng, H. Wu, J. Du, Z. Hu, S. Xu, J. Chen, A. He, B. Liu, D. Wang, Y. G. Yang and J. Wang (2018). "Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes." Nat Immunol 19(3): 279-290.

22.  Weng, Q., F. Hu, M. Zhang, Y. Dong, C. Lv, Y. Wang, X. Liu and J. Wang (2018). "A protocol for generating induced T cells by reprogramming B cells in vivo." Cell Regen 7(1): 7-15.

23.  Wang, T., C. Xia, Y. Dong, X. Chen, J. Wang and J. Du (2018). "Trim27 confers myeloid hematopoiesis competitiveness by up-regulating myeloid master genes." J Leukoc Biol 104(4): 799-809.

24.  Li, X., C. Xia, T. Wang, L. Liu, Q. Zhao, D. Yang, F. Hu, M. Zhang, K. Huang, Y. Geng, Y. Zheng, Y. Guan, H. Wu, X. Chen, G. Pan, J. Chen, J. Du and J. Wang (2017). "Pyrimidoindole derivative UM171 enhances derivation of hematopoietic progenitor cells from human pluripotent stem cells." Stem Cell Res 21: 32-39.

25.  Wang, T., C. Li, C. Xia, Y. Dong, D. Yang, Y. Geng, J. Cai, J. Zhang, X. Zhang and J. Wang (2015). "Oncogenic NRAS hyper-activates multiple pathways in human cord blood stem/progenitor cells and promotes myelomonocytic proliferation in vivo." Am J Transl Res 7(10): 1963-1973.

26.  Chen, X., Q. Zhao, C. Li, Y. Geng, K. Huang, J. Zhang, X. Wang, J. Yang, T. Wang, C. Xia, X. Liu, M. Meng, D. Yang, Y. Zheng, J. Du, X. Zhang, J. Chen, G. Pan and J. Wang (2015). "OP9-Lhx2 stromal cells facilitate derivation of hematopoietic progenitors both in vitro and in vivo." Stem Cell Res 15(2): 395-402.

27.  Yang, D., X. Zhang, Y. Dong, X. Liu, T. Wang, X. Wang, Y. Geng, S. Fang, Y. Zheng, X. Chen, J. Chen, G. Pan and J. Wang (2015). "Enforced expression of Hoxa5 in haematopoietic stem cells leads to aberrant erythropoiesis in vivo." Cell Cycle 14(4): 612-620.

28.  Wang, J., G. Kong, Y. Liu, J. Du, Y. I. Chang, S. R. Tey, X. Zhang, E. A. Ranheim, M. K. Saba-El-Leil, S. Meloche, A. Damnernsawad, J. Zhang and J. Zhang (2013). "Nras(G12D/+) promotes leukemogenesis by aberrantly regulating hematopoietic stem cell functions." Blood 121(26): 5203-5207.

29.  Wang, J., Y. Liu, Z. Li, Z. Wang, L. X. Tan, M. J. Ryu, B. Meline, J. Du, K. H. Young, E. Ranheim, Q. Chang and J. Zhang (2011). "Endogenous oncogenic Nras mutation initiates hematopoietic malignancies in a dose- and cell type-dependent manner." Blood 118(2): 368-379.

30.  Wang, J., Y. Liu, Z. Li, J. Du, M. J. Ryu, P. R. Taylor, M. D. Fleming, K. H. Young, H. Pitot and J. Zhang (2010). "Endogenous oncogenic Nras mutation promotes aberrant GM-CSF signaling in granulocytic/monocytic precursors in a murine model of chronic myelomonocytic leukemia." Blood 116(26): 5991-6002.

Stem cell biology,

Blood and Immune cell biology,

Immune cell theray

已指导学生

李学家  博士研究生  071009-细胞生物学  

董勇  博士研究生  071009-细胞生物学  

张梦云  博士研究生  071009-细胞生物学  

郭荣群  博士研究生  071009-细胞生物学  

夏成祥  博士研究生  071009-细胞生物学  

汪颖  硕士研究生  071009-细胞生物学  

翁启童  硕士研究生  085238-生物工程  

周培青  博士研究生  071009-细胞生物学  

翁启童  博士研究生  071009-细胞生物学  

张琪  博士研究生  071009-细胞生物学  

黄德浩  博士研究生  071009-细胞生物学  

熊加品  博士研究生  071009-细胞生物学  

吴冰燕  博士研究生  071009-细胞生物学  

彭欢  博士研究生  071009-细胞生物学  

李剑焕  博士研究生  071009-细胞生物学  

王瑶  博士研究生  071009-细胞生物学  

现指导学生

肖子芸  博士研究生  0710J6-再生医学  

吴嘉欣  硕士研究生  0710J6-再生医学  

王智乾  博士研究生  1001J6-再生医学  

林云轻  博士研究生  0710J6-再生医学  

徐嘉诚  硕士研究生  0710J6-再生医学  

张辰圆  硕士研究生  0710J6-再生医学  

刘鹏程  博士研究生  1001J6-再生医学  

冯琴琴  硕士研究生  0710J6-再生医学  

伊若文  硕士研究生  0710J6-再生医学  

张乐强  博士研究生  1001J6-再生医学  

赵瑶琴  博士研究生  1001J6-再生医学  

张帆  博士研究生  1001J6-再生医学  

郑秀娟  博士研究生  071009-细胞生物学