基本信息

毕昌昊   中国科学院天津工业生物技术研究所
电子邮件:bi_ch@tib.cas.cn
通信地址:天津市空港经济区西七道32号
邮政编码:300308

研究领域

毕昌昊  中国科学院天津工业生物技术研究所研究员,合成生物技术研究组PI,曾担任国家“863项目首席科学家。

本科及硕士毕业于南开大学,2009年于弗罗里达大学取得博士学位,先后在美国特拉华大学和劳伦斯伯克利国家实验室工作,2014年开始在中科院天津工生所担任研究组PI。主要研究方向为合成生物学技术与基因组编辑技术。现从事合成生物学技术方法的创建与运用,基因组编辑技术研发,人工细胞和细胞机器设计构建。几种代表性的合成生物学和基因组编辑技术方法造成较大影响,已有上百个国内外实验室已索取材料并使用。在主流学术期刊Nature Biotechnology, Metabolic Engeering, ACS Synthetic Biology, Microbial Cell Factories., Chemistry Communications., Journal of Bacteriology., AEM等发表SCI论文40余篇,申请国际专利两项。


招生信息

招收具有分子生物学,细胞生物学,遗传学等研究背景或者有较高兴趣的学生。

招生专业
071005-微生物学
071010-生物化学与分子生物学
071020-生物技术与医药
招生方向
微生物生理学
合成生物学
代谢工程

教育背景

2004-08--美国弗罗里达大学   博士
2001-09--南开大学   硕士
1997-09--南开大学   学士

工作经历

   
工作简历
2014-01~现在, 中国科学院天津工业生物技术研究所, 研究员、教授
2011-09~现在, 美国劳伦斯伯克利国家实验室, 青年研究员
2009-09~现在, 美国特拉华大学, 博士后研究员

教授课程

代谢工程导论

专利与奖励

   
奖励信息
(1) 总统奖学金,一等奖,研究所(学校)级,2005
专利成果
[1] 毕昌昊, 张学礼, 刘丽, 赵东东, 李斯微. 制备串联重复DNA的方法及相关生物材料与应用. CN: CN112080493B, 2021-12-28.

[2] 张学礼, 毕昌昊, 赵东东, 李斯微. 实现C到A以及C到G碱基突变的碱基编辑系统及其应用. CN: CN111763686A, 2020-10-13.

[3] 毕昌昊, 赵东东, 孙宏年. 利用谷氨酸棒状杆菌生产紫色杆菌素的方法. CN: CN107365779A, 2017-11-21.

[4] 张学礼, 李清艳, 唐金磊, 毕昌昊. 生产β‑胡萝卜素的重组微生物及构建方法与应用. CN: CN106434507A, 2017-02-22.

[5] 张学礼, 李清艳, 唐金磊, 毕昌昊. 一种生产番茄红素重组菌的构建方法与应用. CN: CN106434506A, 2017-02-22.

[6] 毕昌昊, 张学礼, 赵东东. 一种用于微生物的CRISPR/Cas9基因编辑载体的构建及其应用. CN: CN105238806A, 2016-01-13.

出版信息

Zhao D., Li J., Li S., Xin, X., Hu M., Price M., Rosser S., Bi, C.* &Zhang, X.* (2020) Glycosylase base editors enable C-to-A and C-to-G base changes. Nature Biotechnology, https://doi.org/10.1038/s41587-020-0592-2 一区

Zhao D, Zhu X, Zhou H, Sun N, Wang T, Bi, C.* &Zhang, X.*. CRISPR-based metabolic pathway engineering. Metab Eng. 2020 Nov 2:S1096-7176(20)30159-2. doi: 10.1016/j.ymben.2020.10.004. PMID: 33152516. 一区

Chen J, Fan F, Qu G, Tang J, Xi Y, Bi C, Sun Z, Zhang X*. Identification of Absidia orchidis steroid 11β-hydroxylation system and its application in engineering Saccharomyces cerevisiae for one-step biotransformation to produce hydrocortisone. Metab Eng. 2020 Jan;57:31-42. doi: 10.1016/j.ymben.2019.10.006. Epub 2019 Oct 24. PMID: 31669370. 一区

Yu Y, Shao M, Li D, Fan F, Xu H, Lu F, Bi C, Zhu X, Zhang X. Construction of a carbon-conserving pathway for glycolate production by synergetic utilization of acetate and glucose in Escherichia coli. Metab Eng. 2020 Sep;61:152-159. doi: 10.1016/j.ymben.2020.06.001. Epub 2020 Jun 10. PMID: 32531344. 一区

Liu L, Zhao D, Ye L, Zhan T, Xiong B, Hu M, Bi, C.* &Zhang, X.*. A programmable CRISPR/Cas9-based phage defense system for Escherichia coli BL21(DE3). Microb Cell Fact. (2020) Jul 3;19(1):136. doi: 10.1186/s12934-020-01393-2. PMID: 32620105; PMCID: PMC7332536. 二区

Li Z, Xin X, Xiong B, Zhao D, Zhang X*, Bi C*. Engineering the Calvin-Benson-Bassham cycle and hydrogen utilization pathway of Ralstonia eutropha for improved autotrophic growth and polyhydroxybutyrate production. Microb Cell Fact. 2020 Dec 11;19(1):228. doi: 10.1186/s12934-020-01494-y. PMID: 33308236; PMCID: PMC7733298. 二区

Xie Q, Li S, Zhao D, Ye L, Li Q, Zhang X, Zhu L*, Bi C*. Manipulating the position of DNA expression cassettes using location tags fused to dCas9 (Cas9-Lag) to improve metabolic pathway efficiency. Microb Cell Fact. 2020 Dec 14;19(1):229. doi: 10.1186/s12934-020-01496-w. PMID: 33317552. 二区

Zhan T, Chen Q, Zhang C, Bi, C.* &Zhang, X.*. Constructing a Novel Biosynthetic Pathway for the Production of Glycolate from Glycerol in Escherichia coli. ACS Synth Biol. 2020 Sep 18;9(9):2600-2609. doi: 10.1021/acssynbio.0c00404. Epub 2020 Sep 1. PMID: 32794740. 二区

Gong Z, Wang H, Tang J, Bi C, Li Q, Zhang X. Coordinated Expression of Astaxanthin Biosynthesis Genes for Improved Astaxanthin Production in Escherichia coli. J Agric Food Chem. 2020 Dec 16;68(50):14917-14927. doi: 10.1021/acs.jafc.0c05379. Epub 2020 Dec 8. PMID: 33289384. 二区

Yu S, Price M., Wang Y, Rosser S., Bi, C.*, and Wang M.* (2020) CRISPR-dCas9 Mediated Cytosine Deaminase Base Editing in Bacillus subtilis. ACS Synthetic Biology 2020 9 (7), 1781-1789 二区

Xin, X., Li, J., Zhao, D., Li, S., Xie, Q., Li, Z., Bi, C.* &Zhang, X*. (2019). Double-check base editing (DBE) for efficient A to G conversions. ACS synthetic biology. (2019).10.1021/acssynbio. 9b00284.

Wang, W., He, P., Zhao, D., Ye, L., Dai, L., Zhang, X*., Bi, C*. (2019). Construction of Escherichia coli cell factories for crocin biosynthesis. Microbial cell factories, 18(1), 120. 二区

Wu, T., Li, S, Zhang, X*,Bi, C*. (2019). Engineering an Artificial Membrane Vesicle Trafficking System (AMVTS) for the Excretion of β-Carotene in Escherichia coli. ACS Synth. Biol.2019.8.1037-1046 二区

Wu, Z., Wang, J., Zhang, Zhang, X*,Bi, C*. (2019). Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate by increasing the intracellular FAD pool. Biochemical Engineering Journal. 146(132-142) 二区

Wu, Z., Wang, J., Liu, J., Wang, Y., Bi, C*., Zhang, X*. (2019). Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO 2. Microbial cell factories18(1), 15. 二区

Li, Z., Xiong, B., Liu, L., Li, S., Xin, X., Li, Z., Zhang, X*, Bi, C*. (2019). Development of an autotrophic fermentation technique for the production of fatty acids using an engineered Ralstonia eutropha cell factory. Journal of industrial microbiology & biotechnology, 1-8. 二区

Ye, L., Zhu, X., Wu, T., Wang, W., Zhao, D., Bi, C. *.,  Zhang, X*. (2018). Optimizing the localization of astaxanthin enzymes for improved productivity. Biotechnology for biofuels, 11(1), 278. 一区

Xiong, B., Li, Z., Liu, L., Zhao, D., Zhang, X*., Bi, C*. (2018). Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique. Biotechnology for Biofuels11(1), 172. 一区

Wu, T., Li, S., Zhang, B., Bi C*, Zhang X*. (2018). Engineering Saccharomyces cerevisiae for the production of the valuable monoterpene ester geranyl acetate. Microbial cell factories17(1), 85. 二区

Wu, T., Ye, L., Zhao, D., Li, S., Li, Q., Zhang, B*., Bi, C*. (2018). Engineering membrane morphology and manipulating synthesis for increased lycopene accumulation in Escherichia coli cell factories. 3 Biotech8(6), 269. 三区

Feng, X., Zhao, D., Zhang, X., Ding, X*., & Bi, C*. (2018). CRISPR/Cas9 assisted Multiplex Genome Editing Technique in Escherichia coli. Biotechnology journal, 1700604. 二区

Zhao, D., Feng, X., Zhu, X., Wu, T., Zhang, X*., and Bi, C*. (2017) CRISPR/Cas9-assisted gRNA-free one-step genome editing with no sequence limitations and improved targeting efficiency, Scientific Reports. 2017.16624. 三区

 

Zhu X, Zhao D, Qiu H, Fan F, Man S, Bi C*, Zhang X*(2017). The CRISPR/Cas9-facilitated multiplex pathway optimization (CFPO) technique and its application to improve the Escherichia coli xylose utilization pathway. Metab Eng. 43:37-45. 一区

 

Wu T, Ye L, Zhao D, Li S, Li Q, Bi C*, Zhang X*. (2017) Membrane engineering - A novel strategy to enhance the production and accumulation of β-carotene in Escherichia coli. Metab Eng., 43: 85-91. 一区

 

Li Q, Fan F, Gao X, Yang C, Bi C, Tang J, Liu T, Zhang X*. (2017) Balanced activation of IspG and IspH to eliminate MEP intermediate accumulation and improve isoprenoids production in Escherichia coli. Metab Eng.08.005 一区

 

Ye L, He P, Li Q, Zhang X*, Bi C*. (2017) Type IIs restriction based combinatory modulation technique for metabolic pathway optimization. Microb Cell Fact. 16:47. 二区

 

Xiao M, Zhu X, Xu H, Tang J, Liu R, Bi C, Fan F*, Zhang X*. (2017) A novel point mutation in RpoB improves osmotolerance and succinic acid production in Escherichia coli. BMC Biotechnology. 17:10 二区

 

Li J, Zhu, X Chen, J Zhao, D., Zhang X*, Bi, C*. (2017) Construction of a novel anaerobic pathway in Escherichia coli for propionate production. BMC biotechnology17(1), 38. 二区

 

Zhao D, Yuan S, Xiong B, Sun H, Ye L, Li J, Zhang X*, Bi C*. (2016) Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9 Microb Cell Fact. 15:205. 二区

 

Ye L, Zhang C, Bi C, Li Q*, Zhang X*. (2016) Combinatory Optimization of Chromosomal Integrated Mevalonate Pathway for β-carotene Production in Escherichia coli. Microb Cell Fact. 2016, 15:202.6 二区

 

Li S, Ding W, Zhang X, Jiang H*, Bi C*. (2016) Development of a modularized two-step (M2S) chromosome integration technique for integration of multiple transcription units in Saccharomyces cerevisiae. Biotechnol Biofuels. 2016, 9:232 一区

 

Sun H, Zhao D, Xiong B, Zhang C*, Bi C*. (2016). Engineering Corynebacterium glutamicum for violacein hyper production. Microb Cell Fact.15(1), 148. 二区

 

C. Bi, J. Mueller, Y. Yeh, N. J. Hillson, H. R. Beller, S. R. Chhabra, and S. W. Singer. (2013) Developing and Applying a Broad-Host Synthetic Biology Tool Box for Metabolic Engineering of Ralstonia eutropha for Hydrocarbon Production. Microb Cell Fact, 12:107 二区

 

C. Bi, S. W. Jones, Dan R. Hess, B. P. Tracy, and E. T. Papoutsakis. (2011) SpoIIE is necessary for asymmetric division, sporulation, and the expression of σF, σE, and σG but does not control solvent production in Clostridium acetobutylicum. J Bacter. 193 no. 19 5130-5137 二区

 

 

C. Bi, J. D Rice, J. F Preston. (2009) Complete fermentation of xylose and methylglucuronoxylose derived from methylglucuronoxylan by Enterobacter asburiae strain JDR-1. Applied and Environmental Microbilogy. (1),395-404 二区

 

 

C. Bi, X. Zhang, L. O. Ingram, J. F Preston. (2009) Genetic engineering Enterobacter asburiae strain JDR-1 for efficient ethanol production from hemicellulose hydrolysate. Applied and Environmental Microbilogy. (9),5743-5749 二区

 

 

C. Bi, X. Zhang, L. O. Ingram, J. F Preston. (2009) Genetic engineering of Enterobacter asburiae strain JDR-1 for efficient latic acid production from hemicellulose hydrolysate. Biotechnology Letters. (31) 1551-1557 三区

 

Y Yuan C. Bi SA. Nicolaou K A. Zingaro M Ralston ET. Papoutsakis Overexpression of the Lactobacillus plantarum peptidoglycan biosynthesis murA2 gene increases the tolerance of Escherichia coli to alcohols and enhances ethanol production. Applied Microbiology and Biotechnology. 2014. 98:8399–8411. 二区

 

G. Linshiz, N. Stawski, S. Poust, C. Bi, J. D. Keasling, and N. J. Hillson. PaR-PaRCross-Platform Laboratory Automation system. ACS Synth. Biol, 2014,3,515-524 二区

 

Y. Yeh, J. Muller, C. Bi, N. J. Hillson, H. R. Beller, S. R. Chhabra, and S. W. Singer. Functionalizing Bacterial Cell Surfaces with a Phage Protein. ChemComm, 2013,49, 910-912一区

G. Linshiz, N. Stawski, S. Poust, C. Bi, J. D. Keasling, and N. J. Hillson. PaR-PaR Laboratory Automation platform. ACS Synth. Biol, 2012,2,216-222 二区

 

G. Linshiz, E. Jensen,N. Stawski, C. Bi, J. D. Keasling, and N. J. Hillson.End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis. Journal of Biological Engineering. 2016. 10:3 二区

 


发表论文
[1] Zhao, Dongdong, Zhu, Xinna, Zhou, Hang, Sun, Naxin, Wang, Ting, Bi, Changhao, Zhang, Xueli. CRISPR-based metabolic pathway engineering. METABOLIC ENGINEERINGnull. 2021, 63: 148-159, http://dx.doi.org/10.1016/j.ymben.2020.10.004.
[2] Xi, Yongyan, Zhan, Tao, Xu, Hongtao, Chen, Jing, Bi, Changhao, Fan, Feiyu, Zhang, Xueli. Characterization of JEN family carboxylate transporters from the acid-tolerant yeast Pichia kudriavzevii and their applications in succinic acid production. MICROBIAL BIOTECHNOLOGY[J]. 2021, 14(3): 1130-1147, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085920/.
[3] Yang, Chao, Li, Zhi, Zhao, Dongdong, Chen, Jing, Zhu, Xinna, Zhang, Xueli, Bi, Changhao. Engineering an efficient H2 utilizing Escherichia coli platform by modulation of endogenous hydrogenases. BIOCHEMICAL ENGINEERING JOURNAL[J]. 2021, 166: http://dx.doi.org/10.1016/j.bej.2020.107851.
[4] Zhao, Dongdong, Li, Ju, Li, Siwei, Xin, Xiuqing, Hu, Muzi, Price, Marcus A, Rosser, Susan J, Bi, Changhao, Zhang, Xueli. Glycosylase base editors enable C-to-A and C-to-G base changes. NATURE BIOTECHNOLOGY[J]. 2021, 39(1): 35-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000607617600017.
[5] Hu, Muzi, Xiong, Bin, Li, Zhongkang, Liu, Li, Li, Siwei, Zhang, Chunzhi, Zhang, Xueli, Bi, Changhao. A novel gene expression system for Ralstonia eutropha based on the T7 promoter. BMC MICROBIOLOGY[J]. 2020, 20(1): http://dx.doi.org/10.1186/s12866-020-01812-9.
[6] Xie, Qianwen, Li, Siwei, Zhao, Dongdong, Ye, Lijun, Li, Qingyan, Zhang, Xueli, Zhu, Li, Bi, Changhao. Manipulating the position of DNA expression cassettes using location tags fused to dCas9 (Cas9-Lag) to improve metabolic pathway efficiency. MICROBIAL CELL FACTORIES[J]. 2020, 19(1): http://dx.doi.org/10.1186/s12934-020-01496-w.
[7] Zhao, Dongdong, Li, Ju, Li, Siwei, Xin, Xiuqing, Hu, Muzi, Price, Marcus A, Rosser, Susan J, Bi, Changhao, Zhang, Xueli. New base editors change C to A in bacteria and C to G in mammalian cells. NATURE BIOTECHNOLOGY. 2020, https://www.webofscience.com/wos/woscc/full-record/WOS:000550630500002.
[8] Yu, Yong, Shao, Mengyao, Li, Di, Fan, Feiyu, Xu, Hongtao, Lu, Fuping, Bi, Changhao, Zhu, Xinna, Zhang, Xueli. Construction of a carbon-conserving pathway for glycolate production by synergetic utilization of acetate and glucose in Escherichia coli. METABOLIC ENGINEERING[J]. 2020, 61: 152-159, http://dx.doi.org/10.1016/j.ymben.2020.06.001.
[9] Chen, Jing, Fan, Feiyu, Qu, Ge, Tang, Jinlei, Xi, Yongyan, Bi, Changhao, Sun, Zhoutong, Zhang, Xueli. Identification of Absidia orchidis steroid 11 beta-hydroxylation system and its application in engineering Saccharomyces cerevisiae for one-step biotransformation to produce hydrocortisone. METABOLIC ENGINEERING[J]. 2020, 57: 31-42, https://www.webofscience.com/wos/woscc/full-record/WOS:000506206200004.
[10] Jing Chen, Feiyu Fan, Ge Qu, Jinlei Tang, Yongyan Xi, Changhao Bi, Zhoutong Sun, Xueli Zhang. Identification of Absidia orchidis steroid 11β-hydroxylation system and its application in engineering Saccharomyces cerevisiae for one-step biotransformation to produce hydrocortisone. Metabolic Engineering. 2020, 57: 31-42, http://dx.doi.org/10.1016/j.ymben.2019.10.006.
[11] Liu, Li, Zhao, Dongdong, Ye, Lijun, Zhan, Tao, Xiong, Bin, Hu, Muzi, Bi, Changhao, Zhang, Xueli. A programmable CRISPR/Cas9-based phage defense system forEscherichia coliBL21(DE3). MICROBIAL CELL FACTORIES[J]. 2020, 19(1): https://doaj.org/article/637ec06a8a48437a80e75c10ce47fef6.
[12] Li Di, Li Yang, Xu JiaoYang, Li QingYan, Tang JinLei, Jia ShiRu, Bi ChangHao, Dai ZhuBo, Zhu XinNa, Zhang XueLi. Engineering CrtW and CrtZ for improving biosynthesis of astaxanthin in Escherichia coli. CHINESE JOURNAL OF NATURAL MEDICINES[J]. 2020, 18(9): 666-676, http://lib.cqvip.com/Qikan/Article/Detail?id=7102969903.
[13] Yu, Sili, Price, Marcus A, Wang, Yu, Liu, Yang, Guo, Yanmei, Ni, Xiaomeng, Rosser, Susan J, Bi, Changhao, Wang, Meng. CRISPR-dCas9 Mediated Cytosine Deaminase Base Editing in Bacillus subtilis. ACS SYNTHETIC BIOLOGY[J]. 2020, 9(7): 1781-1789, https://www.webofscience.com/wos/woscc/full-record/WOS:000551555500027.
[14] Liu, Yan, Pan, Chao, Ye, Lijun, Si, Yue, Bi, Changhao, Hua, Xiaoting, Yu, Yunsong, Zhu, Li, Wang, Hengliang. Nonclassical Biofilms Induced by DNA Breaks in Klebsiella pneumoniae. MSPHERE[J]. 2020, 5(3): https://doaj.org/article/740db1498f7441bc8ae5b7aef620a781.
[15] Li, Zhongkang, Xin, Xiuqing, Xiong, Bin, Zhao, Dongdong, Zhang, Xueli, Bi, Changhao. Engineering the Calvin-Benson-Bassham cycle and hydrogen utilization pathway of Ralstonia eutropha for improved autotrophic growth and polyhydroxybutyrate production. MICROBIAL CELL FACTORIES[J]. 2020, 19(1): http://dx.doi.org/10.1186/s12934-020-01494-y.
[16] Zhan, Tao, Chen, Qian, Zhang, Chao, Bi, Changhao, Zhang, Xueli. Constructing a Novel Biosynthetic Pathway for the Production of Glycolate from Glycerol in Escherichia coli. ACS SYNTHETIC BIOLOGY[J]. 2020, 9(9): 2600-2609, https://www.webofscience.com/wos/woscc/full-record/WOS:000574922400039.
[17] Gong, Zhongkuo, Wang, Honglei, Tang, Jinlei, Bi, Changhao, Li, Qingyan, Zhang, Xueli. Coordinated Expression of Astaxanthin Biosynthesis Genes for Improved Astaxanthin Production in Escherichia coli. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY[J]. 2020, 68(50): 14917-14927, http://dx.doi.org/10.1021/acs.jafc.0c05379.
[18] Wu, Tao, Li, Siwei, Ye, Lijun, Zhao, Dongdong, Fan, Feiyu, Li, Qinyan, Zhang, Bolin, Bi, Changhao, Zhang, Xueli. Engineering an Artificial Membrane Vesicle Trafficking System (AMVTS) for the Excretion of beta-Carotene in Escherichia coil. ACS SYNTHETIC BIOLOGY[J]. 2019, 8(5): 1037-1046, https://www.webofscience.com/wos/woscc/full-record/WOS:000468697000014.
[19] Wu, Zaiqiang, Wang, Junsong, Liu, Jun, Wang, Yan, Bi, Changhao, Zhang, Xueli. Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO2. MICROBIAL CELL FACTORIES[J]. 2019, 18(1): https://doaj.org/article/b1c9dbe392c942618bd9a6d33d726ed9.
[20] Wu, Zaiqiang, Zhao, Dongdong, Li, Siwei, Wang, Junsong, Bi, Changhao, Zhang, Xueli. Combinatorial modulation of initial codons for improved zeaxanthin synthetic pathway efficiency in Escherichia coli. MicrobiologyOpen[J]. 2019, 8(12): n/a-n/a, https://doaj.org/article/501f5bb8bfd34844a071dff4391b1b63.
[21] Li, Zhongkang, Xiong, Bin, Liu, Li, Li, Siwei, Xin, Xiuqing, Li, Zhi, Zhang, Xueli, Bi, ChangHao. Development of an autotrophic fermentation technique for the production of fatty acids using an engineered Ralstonia eutropha cell factory (vol 46, pg 783, 2019). JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGYnull. 2019, 46(6): 791-791, [22] Wu, Zaiqiang, Wang, Junsong, Zhang, Xueli, Bi, Changhao. Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate by increasing the intracellular FAD pool. BIOCHEMICAL ENGINEERING JOURNAL[J]. 2019, 146: 132-142, http://dx.doi.org/10.1016/j.bej.2019.03.015.
[23] Li, Zhongkang, Xiong, Bin, Liu, Li, Li, Siwei, Xin, Xiuqing, Li, Zhi, Zhang, Xueli, Bi, ChangHao. Development of an autotrophic fermentation technique for the production of fatty acids using an engineered Ralstonia eutropha cell factory. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY[J]. 2019, 46(6): 783-790, [24] Huang, Chaoyong, Ding, Tingting, Wang, Jingge, Wang, Xueqin, Guo, Liwei, Wang, Jialei, Zhu, Lin, Bi, Changhao, Zhang, Xueli, Ma, Xiaoyan, Huo, YiXin. CRISPR-Cas9-assisted native end-joining editing offers a simple strategy for efficient genetic engineering in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2019, 103(20): 8497-8509, https://www.webofscience.com/wos/woscc/full-record/WOS:000491439700020.
[25] Xin, Xiuqing, Li, Ju, Zhao, Dongdong, Li, Siwei, Xie, Qianwen, Li, Zhongkang, Fan, Feiyu, Bi, Changhao, Zhang, Xueli. Double-Check Base Editing for Efficient A to G Conversions. ACS SYNTHETIC BIOLOGY[J]. 2019, 8(12): 2629-2634, https://www.webofscience.com/wos/woscc/full-record/WOS:000504805800003.
[26] Wang, Wen, He, Ping, Zhao, Dongdong, Ye, Lijun, Dai, Longhai, Zhang, Xueli, Sun, Yuanxia, Zheng, Jing, Bi, Changhao. Construction of Escherichia coli cell factories for crocin biosynthesis. MICROBIAL CELL FACTORIES[J]. 2019, 18(1): http://dx.doi.org/10.1186/s12934-019-1166-1.
[27] Chen, Jing, Tang, Jinlei, Xi, Yongyan, Dai, Zhubo, Bi, Changhao, Chen, Xi, Fan, Feiyu, Zhang, Xueli. Production of 14 alpha-hydroxysteroids by a recombinant Saccharomyces cerevisiae biocatalyst expressing of a fungal steroid 14 alpha-hydroxylation system. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2019, 103(20): 8363-8374, [28] Guo, JinYing, Hu, KunLe, Bi, ChangHao, Li, QingYan, Zhang, XueLi. Construction of an alternative glycerol-utilization pathway for improved beta-carotene production in Escherichia coli. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY[J]. 2018, 45(8): 697-705, https://www.webofscience.com/wos/woscc/full-record/WOS:000440994500004.
[29] Wu, Tao, Ye, Lijun, Zhao, Dongdong, Li, Siwei, Li, Qingyan, Zhang, Bolin, Bi, Changhao. Engineering membrane morphology and manipulating synthesis for increased lycopene accumulation in Escherichia coli cell factories. 3 BIOTECH[J]. 2018, 8(6): https://www.webofscience.com/wos/woscc/full-record/WOS:000433250900003.
[30] Ye, Lijun, Zhu, Xinna, Wu, Tao, Wang, Wen, Zhao, Dongdong, Bi, Changhao, Zhang, Xueli. Optimizing the localization of astaxanthin enzymes for improved productivity. BIOTECHNOLOGY FOR BIOFUELS[J]. 2018, 11(1): https://doaj.org/article/a1468afa791141129ec252e95028534b.
[31] Xiong, Bin, Li, Zhongkang, Liu, Li, Zhao, Dongdong, Zhang, Xueli, Bi, Changhao. Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique. BIOTECHNOLOGY FOR BIOFUELS[J]. 2018, 11(1): https://doaj.org/article/af6d8195c3d04342aa590e457cd118b1.
[32] 张学礼, 仇焕娜, 赵东东, 满淑丽, 毕昌昊, 朱欣娜. 大肠杆菌染色体上严谨型启动子的构建. 微生物学通报[J]. 2018, 45(8): 1693-1704, http://lib.cqvip.com/Qikan/Article/Detail?id=675941437.
[33] Feng, Xu, Zhao, Dongdong, Zhang, Xueli, Ding, Xiang, Bi, Changhao. CRISPR/Cas9 Assisted Multiplex Genome Editing Technique in Escherichia coli. BIOTECHNOLOGY JOURNAL[J]. 2018, 13(9): https://www.webofscience.com/wos/woscc/full-record/WOS:000443381400014.
[34] Wu, Tao, Li, Siwei, Zhang, Bolin, Bi, Changhao, Zhang, Xueli. Engineering Saccharomyces cerevisiae for the production of the valuable monoterpene ester geranyl acetate. MICROBIAL CELL FACTORIES[J]. 2018, 17(1): https://doaj.org/article/5636cb1d5ee54ba69b6a4bfde9f5e485.
[35] Zhao, Dongdong, Feng, Xu, Zhu, Xinna, Wu, Tao, Zhang, Xueli, Bi, Changhao. CRISPR/Cas9-assisted gRNA-free one-step genome editing with no sequence limitations and improved targeting efficiency. SCIENTIFIC REPORTS[J]. 2017, 7(1): https://doaj.org/article/d91e1517d7464a529c16b5432ef2445f.
[36] Wu, Tao, Ye, Lijun, Zhao, Dongdong, Li, Siwei, Li, Qingyan, Zhang, Bolin, Bi, Changhao, Zhang, Xueli. Membrane engineering - A novel strategy to enhance the production and accumulation of beta-carotene in Escherichia coli. METABOLIC ENGINEERING[J]. 2017, 43: 85-91, https://www.webofscience.com/wos/woscc/full-record/WOS:000410479600010.
[37] 孙宏年, 毕昌昊, 张春枝. 紫色杆菌素在谷氨酸棒状杆菌中异源表达. 大连工业大学学报. 2017, 36(2): 79-83, http://lib.cqvip.com/Qikan/Article/Detail?id=672167542.
[38] Li, Qingyan, Fan, Feiyu, Gao, Xiang, Yang, Chen, Bi, Changhao, Tang, Jinlei, Liu, Tao, Zhang, Xueli. Balanced activation of IspG and IspH to eliminate MEP intermediate accumulation and improve isoprenoids production in Escherichia coli. METABOLIC ENGINEERING[J]. 2017, 44: 13-21, http://dx.doi.org/10.1016/j.ymben.2017.08.005.
[39] Tao Wu, Lijun Ye, Dongdong Zhao, Siwei Li, Qingyan Li, Bolin Zhang, Changhao Bi, Xueli Zhang. Membrane engineering - A novel strategy to enhance the production and accumulation of β-carotene in Escherichia coli. Metabolic Engineering. 2017, 43: 85-91, http://dx.doi.org/10.1016/j.ymben.2017.07.001.
[40] Ye, Lijun, He, Ping, Li, Qingyan, Zhang, Xueli, Bi, Changhao. Type IIs restriction based combinatory modulation technique for metabolic pathway optimization. MICROBIAL CELL FACTORIES[J]. 2017, 16: https://www.webofscience.com/wos/woscc/full-record/WOS:000397738100001.
[41] Zhu, Xinna, Zhao, Dongdong, Qiu, Huanna, Fan, Feiyu, Man, Shuli, Bi, Changhao, Zhang, Xueli. The CRISPR/Cas9-facilitated multiplex pathway optimization (CFPO) technique and its application to improve the Escherichia coli xylose utilization pathway. METABOLIC ENGINEERING[J]. 2017, 43: 37-45, http://dx.doi.org/10.1016/j.ymben.2017.08.003.
[42] Xiao, Mengyong, Zhu, Xinna, Bi, Changhao, Ma, Yanhe, Zhang, Xueli. Improving Succinate Productivity by Engineering a Cyanobacterial CO2 Concentrating System (CCM) in Escherichia coli. BIOTECHNOLOGY JOURNAL[J]. 2017, 12(9): https://www.webofscience.com/wos/woscc/full-record/WOS:000411380100008.
[43] Li Qingyan, Fan Feiyu, Gao Xiang, Yang Chen, Bi Changhao, Tang Jinlei, Liu Tao, Zhang Xueli. Balanced activation of IspG and IspH to eliminate MEP intermediate accumulation and improve isoprenoids production in Escherichia coli. Metabolic Engineering[J]. 2017, [44] Xiao, Mengyong, Zhu, Xinna, Xu, Hongtao, Tang, Jinlei, Liu, Ru, Bi, Changhao, Fan, Feiyu, Zhang, Xueli. A novel point mutation in RpoB improves osmotolerance and succinic acid production in Escherichia coli. BMC BIOTECHNOLOGY[J]. 2017, 17(1): http://www.corc.org.cn/handle/1471x/2374341.
[45] Li, Jing, Zhu, Xinna, Chen, Jing, Zhao, Dongdong, Zhang, Xueli, Bi, Changhao. Construction of a novel anaerobic pathway in Escherichia coli for propionate production. BMC BIOTECHNOLOGY[J]. 2017, 17(1): http://dx.doi.org/10.1186/s12896-017-0354-5.
[46] Li, Siwei, Ding, Wentao, Zhang, Xueli, Jiang, Huifeng, Bi, Changhao. Development of a modularized two-step (M2S) chromosome integration technique for integration of multiple transcription units in Saccharomyces cerevisiae. BIOTECHNOLOGY FOR BIOFUELS[J]. 2016, 9(1): http://dx.doi.org/10.1186/s13068-016-0645-4.
[47] Zhao, Dongdong, Yuan, Shenli, Xiong, Bin, Sun, Hongnian, Ye, Lijun, Li, Jing, Zhang, Xueli, Bi, Changhao. Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9. MICROBIAL CELL FACTORIES[J]. 2016, 15: http://124.16.173.210/handle/834782/2288.
[48] Linshiz, Gregory, Jensen, Erik, Stawski, Nina, Bi, Changhao, Elsbree, Nick, Jiao, Hong, Kim, Jungkyu, Mathies, Richard, Keasling, Jay D, Hillson, Nathan J. End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis. JOURNAL OF BIOLOGICAL ENGINEERING[J]. 2016, 10(1): http://dx.doi.org/10.1186/s13036-016-0024-5.
[49] Lijun Ye, Chunzhi Zhang, Changhao Bi, Qingyan Li, Xueli Zhang. Combinatory optimization of chromosomal integrated mevalonate pathway for β-carotene production in Escherichia coli. Microbial Cell Factories. 2016, 15: [50] Sun, Hongnian, Zhao, Dongdong, Xiong, Bin, Zhang, Chunzhi, Bi, Changhao. Engineering Corynebacterium glutamicum for violacein hyper production. MICROBIAL CELL FACTORIES[J]. 2016, 15(1): http://124.16.173.210/handle/834782/2932.
[51] Ye, Lijun, Zhang, Chunzhi, Bi, Changhao, Li, Qingyan, Zhang, Xueli. Combinatory optimization of chromosomal integrated mevalonate pathway for beta-carotene production in Escherichia coli. MICROBIAL CELL FACTORIES[J]. 2016, 15: http://124.16.173.210/handle/834782/2289.
[52] Linshiz, Gregory, Stawski, Nina, Goyal, Garima, Bi, Changhao, Poust, Sean, Sharma, Monica, Mutalik, Vivek, Keasling, Jay D, Hillson, Nathan J. PR-PR: Cross-Platform Laboratory Automation System. ACS SYNTHETIC BIOLOGY[J]. 2014, 3(8): 515-524, http://124.16.173.210/handle/834782/2267.
[53] Yuan, Yongbo, Bi, Changhao, Nicolaou, Sergios A, Zingaro, Kyle A, Ralston, Matthew, Papoutsakis, Eleftherios T. Overexpression of the Lactobacillus plantarum peptidoglycan biosynthesis murA2 gene increases the tolerance of Escherichia coli to alcohols and enhances ethanol production. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2014, 98(19): 8399-8411, https://www.webofscience.com/wos/woscc/full-record/WOS:000342072500030.
[54] 毕昌昊. Developing and Applying a Broad-Host Synthetic Biology Tool Box for Metabolic Engineering of Ralstonia eutropha for Hydrocarbon Production.. Microb Cell Fact. 2013, [55] Linshiz, Gregory, Stawski, Nina, Poust, Sean, Bi, Changhao, Keasling, Jay D, Hilson, Nathan J. PaR-PaR Laboratory Automation Platform. ACS SYNTHETIC BIOLOGY[J]. 2013, 2(5): 216-222, https://www.webofscience.com/wos/woscc/full-record/WOS:000321074300004.
[56] Yeh, YiChun, Mueller, Jana, Bi, Changhao, Hillson, Nathan J, Beller, Harry R, Chhabra, Swapnil R, Singer, Steven W. Functionalizing bacterial cell surfaces with a phage protein. CHEMICAL COMMUNICATIONS[J]. 2013, 49(9): 910-912, https://www.webofscience.com/wos/woscc/full-record/WOS:000312944700019.
[57] 毕昌昊. SpoIIE is necessary for asymmetric division, sporulation, and the expression of σF, σE, and σG but does not control solvent production in Clostridium acetobutylicum.. J. Bacteriol.. 2011, [58] 毕昌昊. Genetic engineering Enterobacter asburiae strain JDR-1 for efficient ethanol production from hemicellulose hydrolysate. Applied and Environmental Microbilogy. 2009, [59] Bi, Changhao, Rice, John D, Preston, James F. Complete Fermentation of Xylose and Methylglucuronoxylose Derived from Methylglucuronoxylan by Enterobacter asburiae Strain JDR-1. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2009, 75(2): 395-404, https://www.webofscience.com/wos/woscc/full-record/WOS:000262197700014.
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科研活动

   
科研项目
(1) 先进生物制造工程菌株的构建与应用,参与,院级级,2014-01--2015-12

合作情况

   
项目协作单位

诺和诺德中国研究中心等

指导学生

已指导学生

李智  硕士研究生  071010-生物化学与分子生物学  

熊斌  博士研究生  071010-生物化学与分子生物学  

现指导学生

叶立军  博士研究生  071010-生物化学与分子生物学  

王婷  硕士研究生  071010-生物化学与分子生物学  

李博  博士研究生  071010-生物化学与分子生物学  

樊旭倩  博士研究生  071010-生物化学与分子生物学  

代英才  硕士研究生  086000-生物与医药  

费吉东  硕士研究生  086000-生物与医药