研究方向为生物大分子的合成，聚焦工业酶、结构蛋白的高效表达及多糖生物合成研究。

   

071010-生物化学与分子生物学
071005-微生物学
0710Z1-基因组学

代谢工程，合成生物学

2010-09--2013-07   中国科学院微生物研究所   博士
2001-09--2004-07   中国科学院动物研究所   硕士
1997-09--2001-07   山西大学   本科

   

2021-04-15-2024-04-15,COMSATS工业生物技术联合中心, 生物基材料联合研究组协调人

   

（ 1 ） 一种类丝弹性蛋白水凝胶的制备方法及其应用, 发明专利, 2022, 第 1 作者, 专利号: 202211614773.X

（ 2 ） 一种利用出芽短梗霉生产中分子量普鲁兰多糖的方法, 发明专利, 2022, 第 1 作者, 专利号: ZL202211106612.X

（ 3 ） 一种普鲁兰多糖形状记忆材料的制备方法及其4D打印方法及制品, 发明专利, 2022, 第 1 作者, 专利号: 202210480388.4

（ 4 ） 一株高产高分子量普鲁兰多糖的出芽短梗霉及其应用, 发明专利, 2022, 第 2 作者, 专利号: 202210480946.7

（ 5 ） 一株高产γ-聚谷氨酸的耐盐芽孢杆菌及其发酵条件优化, 发明专利, 2022, 第 2 作者, 专利号: 202210374606.6

（ 6 ） 一种增强丝蛋白生产制备的表达系统, 专利授权, 2018, 第 2 作者, 专利号: CN107723287A

（ 7 ） 一种碱性木聚糖酶及其编码基因与应用, 发明专利, 2015, 第 2 作者, 专利号: CN104630184A

（ 8 ） 一种耐盐木聚糖酶及其编码基因与应用, 发明专利, 2013, 第 2 作者, 专利号: CN103060289A

（ 9 ） 一株可降解有机磷农药的降解菌及其用途, 发明专利, 2007, 第 2 作者, 专利号: CN1306023

   

[1] JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2024, 第 5 作者  通讯作者  
[2] ACS Synthetic Biology. 2024, 第 5 作者  通讯作者  
[3] Zhaoxuan Feng, Sijia Wang, Wenxin Huang, Wenqin Bai. A potential bilayer skin substitute based on electrospun silk-elastin-like protein nanofiber membrane covered with bacterial cellulose. COLLOIDS AND SURFACES B: BIOINTERFACES. 2024, 第 4 作者  通讯作者  234: http://dx.doi.org/10.1016/j.colsurfb.2023.113677.
[4] BMC BIOTECHNOLOGY. 2024, 第 9 作者  通讯作者  
[5] Colloids and Surfaces B: Biointerfaces. 2024, 第 4 作者  通讯作者  
[6] Chen, Shuyu, Zheng, Hongchen, Gao, Jiaqi, Song, Hui, Bai, Wenqin. High-level production of pullulan and its biosynthesis regulation in Aureobasidium pullulans BL06. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY[J]. 2023, 第 5 作者  通讯作者  11: http://dx.doi.org/10.3389/fbioe.2023.1131875.
[7] Yifan Yang, Xiaoping Fu, Xingya Zhao, Jianyong Xu, Yihan Liu, Hongchen Zheng, Wenqin Bai, Hui Song. Overexpression of a Thermostable α-Amylase through Genome Integration in Bacillus subtilis. FERMENTATION[J]. 2023, 第 7 作者9(139): https://doaj.org/article/14e56a415bec48d99818b18a7a0a070c.
[8] Wang, Sijia, Huang, Wenxin, Feng, Zhaoxuan, Tian, Xiaoli, Wang, Dexin, Rao, Lang, Tan, Ming, Roongsawang, Niran, Song, Hui, Jiang, Wenxia, Bai, Wenqin. Laccase-mediated formation of hydrogels based on silk-elastin-like protein polymers with ultra-high molecular weight. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES[J]. 2023, 第 11 作者231: http://dx.doi.org/10.1016/j.ijbiomac.2023.123239.
[9] Feng, Zhaoxuan, Li, Jinsong, Zhou, Dasen, Song, Hui, Lv, Jiaqi, Bai, Wenqin. A novel photocurable pullulan-based bioink for digital light processing 3D printing. INTERNATIONAL JOURNAL OF BIOPRINTING[J]. 2023, 第 6 作者  通讯作者  9(2): 104-117, 
[10] Wang, Dexin, Fu, Xiaoping, Zhou, Dasen, Gao, Jiaqi, Bai, Wenqin. Engineering of a newly isolated Bacillus tequilensis BL01 for poly-?-glutamic acid production from citric acid. MICROBIAL CELL FACTORIES[J]. 2022, 第 5 作者  通讯作者  21(1): http://dx.doi.org/10.1186/s12934-022-01994-z.
[11] Feng, Zhaoxuan, Chen, Shuyu, Ahmad, Abdullah, Chen, Longjiao, Bai, Wenqin. Ultra-high molecular weight pullulan-based material with high deformability and shape-memory properties. CARBOHYDRATE POLYMERS[J]. 2022, 第 5 作者  通讯作者  295: http://dx.doi.org/10.1016/j.carbpol.2022.119836.
[12] Bai Wenqin, Winston E Anthony, Gautam Dantas, Fuzhong Zhang. Engineering diverse fatty acid compositions of phospholipids in Escherichia coli. Metabolic Engineering[J]. 2022, 第 1 作者74: 11-23, https://pubmed.ncbi.nlm.nih.gov/36058465/.
[13] 郑宏臣, 徐健勇, 杨建花, 郑迎迎, 涂然, 石婷, 付刚, 刘倩, 王兴彪, 韩旭, 张以恒, 柏文琴, 宋诙, , , , , , , , , , , , , . 工业酶与绿色生物工艺的核心技术进展. 生物工程学报[J]. 2022, 第 0 作者38(11): 4219-4239, https://cjb.ijournals.cn/cjbcn/article/abstract/gc22114219?st=article_issue.
[14] Wenqin Bai, Cameron J. Sargent, JeongMo Choi, Rohit V. Pappu, Fuzhong Zhang. Covalently-assembled single-chain protein nanostructures with ultra-high stability. NATURE COMMUNICATIONS[J]. 2019, 第 1 作者10(1): 1-10, 
[15] Wenqin Bai, Weitao Geng, Shaojie Wang, Fuzhong Zhang. Biosynthesis, regulation, and engineering of microbially produced branched biofuels. BIOTECHNOLOGY FOR BIOFUELS[J]. 2019, 第 1 作者12(1): 1-12, https://doaj.org/article/a9f61764fffb4849958b9e3664a4e385.
[16] Wang, Jingyu, Wang, Jilong, Tai, Yishu, Zhang, Qiuge, Bai, Wenqin, Zhang, Kechun. Rerouting carbon flux for optimized biosynthesis of mesaconate in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2018, 第 5 作者102(17): 7377-7388, https://www.webofscience.com/wos/woscc/full-record/WOS:000441229200014.
[17] Bowen, Christopher H, Dai, Bin, Sargent, Cameron J, Bai, Wenqin, Ladiwala, Pranay, Feng, Huibao, Huang, Wenwen, Kaplan, David L, Galazka, Jonathan M, Zhang, Fuzhong. Recombinant Spidroins Fully Replicate Primary Mechanical Properties of Natural Spider Silk. BIOMACROMOLECULES[J]. 2018, 第 4 作者19(9): 3853-3860, https://www.webofscience.com/wos/woscc/full-record/WOS:000444527300024.
[18] Bai, Wenqin, Cao, Yufan, Liu, Jun, Wang, Qinhong, Jia, Zhenhu. Improvement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp SN5 by random mutation and Glu135 saturation mutagenesis. BMC BIOTECHNOLOGY[J]. 2016, 第 1 作者  通讯作者  16(1): http://www.irgrid.ac.cn/handle/1471x/1180901.
[19] Bai, Wenqin, Tai, YiShu, Wang, Jingyu, Wang, Jilong, Jambunathan, Pooja, Fox, Kevin J, Zhang, Kechun. Engineering nonphosphorylative metabolism to synthesize mesaconate from lignocellulosic sugars in Escherichia coli. METABOLIC ENGINEERING[J]. 2016, 第 1 作者38: 285-292, http://dx.doi.org/10.1016/j.ymben.2016.09.007.
[20] Bo Wenqin. Engineering Highly Efficient E. coli strain for Mevalonate Fermentation by Chromosomal Integration. Applied and Environmental Microbiology. 2016, 第 1 作者
[21] Bai Wenqin. Engineering Nonphosphorylative Metabolism to Synthesize Mesaconate from D-Xylose and L-Arabinose in Escherichia coli. Metabolic Engineering[J]. 2016, 第 1 作者
[22] Bai, Wenqin, Zhou, Cheng, Zhao, Yueju, Wang, Qinhong, Ma, Yanhe. Structural Insight into and Mutational Analysis of Family 11 Xylanases: Implications for Mechanisms of Higher pH Catalytic Adaptation. PLOS ONE[J]. 2015, 第 1 作者  通讯作者  10(7): http://dx.doi.org/10.1371/journal.pone.0132834.
[23] Bai, Wenqin, Xue, Yanfen, Zhou, Cheng, Ma, Yanhe. Cloning, expression, and characterization of a novel alkali-tolerant xylanase from alkaliphilic Bacillus sp SN5. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY[J]. 2015, 第 1 作者62(2): 208-217, http://www.irgrid.ac.cn/handle/1471x/1111534.
[24] 柏文琴, 王钦宏, 马延和, , , , . 嗜热和嗜碱木聚糖酶研究进展. 生物工程学报[J]. 2014, 第 1 作者30(6): 828-837, https://cjb.ijournals.cn/cjbcn/article/abstract/gc14060828?st=article_issue.
[25] Bai, Wenqin, Zhou, Cheng, Xue, Yanfen, Huang, ChunHsiang, Guo, ReyTing, Ma, Yanhe. Three-dimensional structure of an alkaline xylanase Xyn11A-LC from alkalophilic Bacillus sp SN5 and improvement of its thermal performance by introducing arginines substitutions. BIOTECHNOLOGY LETTERS[J]. 2014, 第 1 作者36(7): 1495-1501, http://www.irgrid.ac.cn/handle/1471x/1111483.
[26] 柏文琴, 杨鲁红, 马延和, , , , . 通过N端引入芳香族氨基酸提高木聚糖酶的热稳定性. 生物工程学报[J]. 2014, 第 1 作者30(8): 1217-1224, https://cjb.ijournals.cn/cjbcn/article/abstract/gc14081217?st=article_issue.
[27] Bai, Wenqin, Xue, Yanfen, Zhou, Cheng, Ma, Yanhe. Cloning, expression and characterization of a novel salt-tolerant xylanase from Bacillus sp SN5. BIOTECHNOLOGY LETTERS[J]. 2012, 第 1 作者34(11): 2093-2099, http://www.irgrid.ac.cn/handle/1471x/1111518.
[28] Zhong, Qiuzan, Zhang, Haiyan, Bai, Wenqin, Li, Mei, Li, Baotong, Qiu, Xinghui. Degradation of aromatic compounds and degradative pathway of 4-nitrocatechol by Ochrobactrum sp B2. JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING[J]. 2007, 第 3 作者42(14): 2111-2116, https://www.webofscience.com/wos/woscc/full-record/WOS:000251705600006.
[29] Qiu, Xinghui, Zhong, Qiuzan, Li, Mei, Bai, Wenqin, Li, Baotong. Biodegradation of p-nitrophenol by methyl parathion-degrading Ochrobactrum sp B2. INTERNATIONAL BIODETERIORATION & BIODEGRADATION[J]. 2007, 第 4 作者59(4): 297-301, http://dx.doi.org/10.1016/j.ibiod.2006.09.005.
[30] Qiu, X H, Bai, W Q, Zhong, Q Z, Li, M, He, F Q, Li, B T. Isolation and characterization of a bacterial strain of the genus Ochrobactrum with methyl parathion mineralizing activity. JOURNAL OF APPLIED MICROBIOLOGY[J]. 2006, 101(5): 986-994, https://www.webofscience.com/wos/woscc/full-record/WOS:000241199100002.
[31] 柏文琴, 李梅, 邱星辉, 何凤琴, 冷欣夫. 苍白杆菌B2对甲基对硫磷降解途径研究. 农药学学报[J]. 2004, 第 1 作者6(4): 48-54, http://lib.cqvip.com/Qikan/Article/Detail?id=11388174.
[32] 柏文琴, 何凤琴, 邱星辉. 有机磷农药生物降解研究进展. 应用与环境生物学报[J]. 2004, 第 1 作者10(5): 675-680, http://lib.cqvip.com/Qikan/Article/Detail?id=10852471.

   

（ 1 ） 基于丝蛋白的功能材料的设计与合成, 负责人, 地方任务, 2020-06--2023-05
（ 2 ） 11家族木聚糖酶嗜碱分子机制研究, 负责人, 国家任务, 2015-01--2017-12
（ 3 ） 多尺度高效编辑重构细胞关键技术体系, 负责人, 国家任务, 2021-07--2025-06
（ 4 ） 基于耐热多形汉逊酵母建立蛋白材料合成新型平台, 负责人, 中国科学院计划, 2023-01--2025-12
（ 5 ） 高性能类丝蛋白的微生物高效合成, 负责人, 地方任务, 2024-01--2025-12

现指导学生

高佳琪  博士研究生  071010-生物化学与分子生物学