基本信息
吴思  女    中国科学院生物物理研究所
电子邮件: wusi@moon.ibp.ac.cn
通信地址: 北京市朝阳区大屯路15号
邮政编码:

招生信息

   
招生专业
071011-生物物理学
招生方向
单分子生物物理学

教育背景

2005-09--2011-01   北京大学   博士
2001-09--2005-07   南开大学   学士

工作经历

   
工作简历
2011-03~现在, 中国科学院生物物理研究所, 副研究员
2005-09~2011-01,北京大学, 博士
2001-09~2005-07,南开大学, 学士

教授课程

生物物理学实验

出版信息

   
发表论文
(1) PES inhibits human inducible Hsp70 by covalent targeting of cysteine residues in the substrate binding domain, J. Biol. Chem, 2021, 第 3 作者
(2) Single molecule characterization of amyloid oligomers, Molecules, 2021, 通讯作者
(3) Studying protein folding in health and disease using biophysical approaches, Emerg. Top. Life Sci., 2021, 第 3 作者
(4) Conformational expansion of Tau in condensates promotes irreversible aggregation, J. Am. Chem. Soc., 2021, 通讯作者
(5) Distinct microscopic mechanism for the accelerated aggregation of pathogenic Tau mutants revealed by kinetics study, Phys. Chem. Chem. Phys., 2020, 通讯作者
(6) Kinetics of the conformational cycle of Hsp70 reveals the importance of the dynamic and heterogeneous nature of Hsp70 for its function, Proc. Nat. Acad. Sci., 2020, 第 1 作者
(7) Amelioration of aggregate cytotoxicity by catalytic conversion of protein oligomers into amyloid fibrils, Nanoscale, 2020, 通讯作者
(8) S-Glutathionylation of human inducible Hsp70 reveals a regulation mechanism involving the C-terminal alpha-helical lid, J. Biol. Chem., 2020, 第 9 作者
(9) Kinetic diversity of amyloid oligomers, Proc. Nat. Acad. Sci., 2020, 第 5 作者
(10) The C-terminal GGAP motif of Hsp70 mediates substrate recognition and stress response in yeast, J. Biol. Chem., 2018, 第 5 作者
(11) Direct observation of oligomerization by single molecule fluorescence reveals a multistep aggregation mechanism for the yeast prion protein Ure2, J. Am. Chem. Soc., 2018, 通讯作者
(12) A co-expression strategy to achieve labeling of individual subunits within a dimeric protein for single molecule analysis, Chem. Comm., 2017, 通讯作者
(13) Glutathionylation of the bacterial Hsp70 chaperone DnaK provides a link between oxidative stress and the heat shock response, J. Biol. Chem., 2017, 第 3 作者
(14) Enzymatically active microgels from self-assembling protein nanofibrils for microflow chemistry, ACS nano, 2015, 第 4 作者
(15) Influence of specific Hsp70 domains on fibril formation of the yeast prion protein Ure2, Phil. Trans. R. Soc. B, 2013, 第 1 作者
发表著作
Protein microgels from amyloid fibril networks, In book: Biological and Bio-inspired Nanomaterials, Nature-Springer, 2019-12, 第 3 作者

科研活动

   
科研项目
( 1 ) 酵母prion蛋白Ure2的寡聚及构象变化的单分子水平研究, 负责人, 国家任务, 2017-01--2020-12
( 2 ) 单分子荧光技术结合动力学分析手段研究淀粉样纤维化及其加速和抑制机制, 参与, 国家任务, 2020-01--2024-12
( 3 ) 人源组成型与应激型Hsp70差异机制的单分子研究, 负责人, 国家任务, 2022-01--2025-12
参与会议
(1)A mechanistic view of the liquid phase separation of Tau at single-molecule level   第十九次中国暨国际生物物理大会   2021-07-23
(2)Conformational expansion of Tau during liquid phase separation facilitates intermolecular interactions and self-aggregation   2021-06-23
(3)Revealing the mechanism of amyloid fibril formation by combined single molecule FRET and kinetic modeling   第六届全国生物物理化学会议   2018-07-22
(4)Revealing the mechanism of amyloid fibril formation by combined single molecule FRET and kinetic modeling   第十五次中国暨国际生物物理大会   2017-10-24
(5)A two-step mechanism of amyloid formation revealed by direct observation of oligomerization of the yeast prion protein Ure2   第十四次中国暨国际生物物理大会   2015-11-17