General

Jian-Jun Li

Associate Professor, PhD

Institute of Process Engineering, the Chinese Academy of Sciences

Corresponding address:  No. 1 North 2nd Street, Haidian District, Beijing 100190, China

Email: jjli@ipe.ac.cn



Research Areas

1.Enzymatic synthesis of functional oligosaccharides.

2. Enzymology and protein engineering of important glycosidases and glycosyltransferases.



Education

1997/9–2001/7, Institute of Chemistry, the Chinese Academy of Sceinces, organic & bioorganic chemistry, PhD

1994/9–1997/9, Fujian Institute of Research on the Structure of Matter, the Chinese Academy of Sceinces, inorganic chemistry, MS

Experience

Enzymatic synthesis of functional oligosaccharides; Enzymology, protein engineering

Work Experience
2014-06~Present, Institute of Process Engineering, the Chinese Academy of Sciences, Associate Professor
2009-12~2014-05,Qingdao Institute of Bioenergy and Bioprocess Technology, the Chinese Academy of Sciences, Associate Professor
2007-12~2009-12,Illumina Ltd (UK), Research Scientist
2001-11~2007-11,Department of Chemistry, University of Warwick (UK), Postdoctoral research fellow


Publications

Representative publications:

  1.  Chen T, Cheng G, Jiao S, Ren L, Zhao C, Wei J, Han J, Pei M*, Du Y*, Li J-J*, Expression and biochemical characterization of a novel marine chitosanase from Streptomyces niveus suitable for preparation of chitobiose, Mar. Drugs, 2021, 19, 300.

  2.  Zhu C, Liu Z, Ren L, Jiao S, Zhang X, Wang Q, Li Z*, Du Y*, Li J-J*, Overexpression and biochemical characterization of a truncated endo-a (1®3)-fucoidanase from Alterоmonas sp. SN-1009, Food Chemistry, 2021, 353, 129460.

  3.  Gong M, Li Ti, Wu L, Zhang Z, Ren L, Duan X, Cao H, Pei M*, Li J-J*, Du Y, Liquid-phase and ultrahigh-frequency-acoustofluidics-based solid-phase synthesis of biotin tagged 6’/3’-sialyl-N-acetylglucosamine by sequential one-pot multienzyme system, Catalysts, 2020, 10, 1347.

  4.  Zhu L, Li R, Jiao S, Wei J, Yan Y, Wang ZA*, Li J-J*, Du Y*, Blood-Brain Barrier Permeable Chitosan Oligosaccharides Interfere with β-amyloid Aggregation and Alleviate β-Amyloid Protein Mediated Neurotoxicity and Neuroinflammation in a Dose- and Degree of Polymerization-Dependent Manner, Mar. Drugs 2020, 18, 488

  5. Liu D, Jiao S, Wei J, Zhang X, Pei Y, Pei Z*, Li J-J*, Du Y*, Investigation of Absorption, Metabolism and Toxicity of Ginsenosides compound K Based on Human Organ Chips, Int. J.Pharmaceut., 2020, 587, 119669

  6.  Jing B, Wang ZA, Zhang C, Deng Q, Wei J, Luo Y, Zhang X*, Li J-J* and Du Y*, Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host–Microbial Interaction. Front. Bioeng. Biotechnol. 2020, 8, 272.

  7.  Xu H, Zhang P, Zhang Y, Liu Z, Zhang X, Li Z*, Li J-J*, Du Y, Overexpression and Biochemical Characterization of an Endo-a-1,4-polygalacturonase from Aspergillus nidulans in Pichia pastoris, Int. J. Mol. Sci., 2020, 21, 2100.

  8. Jing B, Cheng G, Li J-J*, Wang ZA*, Du Y*, Inhibition of Liver Tumor Cell Metastasis by Partially Acetylated Chitosan Oligosaccharide on A Tumor-Vessel Microsystem, Mar. Drugs, 2019, 17, 415.

  9.  Jing B, Luo Y, Lin B, Li J-J*, Wang ZA*, Du Y*, Establishment and application of a dynamic tumor-vessel microsystem for studying different stages of tumor metastasis and evaluating anti-tumor drugs. RSC Adv., 2019, 9, 17137-17147. 

  10. Ye J, Zhao Y*, Du Y*, Li J-J*, Cloning, overexpression and characterization of isomaltases IMA1 from different strains of Saccharomyces cerevisiae, Acta Microbiologica Sinica, 2019, 59, 1354-1362.

  11.  Yuan X, Zhang P, Jiao S, Du Y*, Li J-J*, Overexpression and biochemical characterization of a recombinant psychrophilic endocellulase from Pseudoalteromonas sp. DY3. Int. J. Biol. Macromol., 2018, 116, 100-105.

  12.  Zhang P, Yuan X*, Du Y*, Li J-J*, Heterologous expression and biochemical characterization of a GHF9 endoglucanse from the termite Reticulitermes speratus in Pichia pastoris. BMC Biotechnology, 2018, 18, 35.

  13.  Song Y, Fu G, Dong H, Li J-J*, Du Y, Zhang D*, High-Efficiency Secretion of β-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization. J. Agric. Food Chem., 2017, 65(12), 2540-2548.

  14. Yu Gao, Jian-Jun Li*, Lanyan Zheng,* Yuguang Du, Rational design of Pleurotus eryngii versatile ligninolytic peroxidase for enhanced pH and thermal stability through structure-based protein engineering, Protein Eng Des Sel., 2017, 30, 743-751 (Co-corresponding authour))

  15. Gao Y, Zheng L*, Li J-J*, Du Y*, Insight into the impact of two structural calcium ions on the properties of Pleurotus eryngii versatile ligninolytic peroxidase, Arch Biochem Biophys, 2016, 612, 9-16. (Co-corresponding author)

  16.  Bao, L, Li, J-J*, Jia, C, Li, M, Lu, X, “Structure-Oriented Substrate Specificity Engineering of Aldehyde-Deformylating Oxygenase towards Aldehydes Carbon Chain Length”, Biotechnol. Biofuels, 2016, 9, 185. (Co-corresponding author)

  17. Chen, M, Huang, X, Zhong, C, Li, J-J, Lu, X, “Identification of an itaconic acid degrading pathway in itaconic acid producing Aspergillus terreus”, Appl. Microbiol. Biotechnol., 2016, 100, 7541-7548.

  18. Huang X, Chen M, Li J-J, Lu X, Establishing an efficient gene-targeting system in an itaconic-acid producing Aspergillus terreus strain, Biotechnol Lett. 2016, 38, 1603–1610.

  19. Jia C, Li M, Li, J-J,, Zhang, J, Zhang, H, Cao, P, Pan, X, Lu, X, Chang, W, “Structural insights into the catalytic mechanism of aldehyde-deformylating oxygenases”, Protein Cell 2014, 6:55-67.

  20. Huang, X, Lu, X, Li, Y, Zhang, X, Li, X, Li, J-J*, “Improving itaconic acid production through genetic engineering of an industrial Aspergillus terreus strain”, Microb. Cell Fact., 2014, 13, 119. (Corresponding author)

  21. Huang, X, Lu, X, Li, Y, Zhang, X, Li, X, Li, J-J*, “Direct production of itaconic acid from liquefied corn starch by genetically engineered Aspergillus terreus”, Microb. Cell Fact., 2014, 13, 108. (Corresponding author)

  22. Wang, Q, Huang, X, Zhang, J, Lu, X, Li, S, Li, J-J*, “Engineering self-sufficient aldehyde deformylating oxygenase fused to alternative electron transfer systems for efficient conversion of aldehydes into alkanes”, Chem. Commun., 2014, 50, 4299-4301. (Corresponding author)

  23. Zhang, J, Lu, X*, Li, J-J*, “Conversion of fatty aldehydes into alk(a/e)nes by in vitro reconstituted cyanobacterial aldehyde deformylating oxygenase with a cognate electron transfer system”, Biotechnol. Biofuels, 2013, 6, 86. (Co-corresponding author)

  24. Bao, X, Huang, X, Lu, X, Li, J-J*, “Improvement of H2O2 stability of Pleurotus eryngii versatile peroxidise through rational protein engineering”, Enzyme Microb. Tech., 2014, 54, 51-58. (Corresponding author)

  25. Huang, X, Lu, X, Li, J-J*, “Cloning, characterization and application of a glyceraldehyde-3-phosphate dehydrogenase promoter from Aspergillus terreus”,   J. Ind. Microbiol. Biot., 2014,  41, 585-592. (Corresponding author)

  26. Li, J-J, Bugg, TDH*, “Investigation of a general base mechanism for ester hydrolysis in C–C hydrolase enzymes of the α/β-hydrolase superfamily: a novel mechanism for the serine catalytic triad”, Org. Biomol. Chem., 2007, 5, 507-513.

  27. Li, J-J, Li, C, Blindauer, CA, Bugg, TDH*, “Evidence for a gem-diol reaction intermediate in bacterial C-C hydrolase enzymes BphD and MhpC from 13C NMR spectroscopy”, Biochemistry, 2006, 45, 12461–12469.

  28. Li, J-J, Bugg, TDH*, “Stereochemistry of the reaction catalysed by 2-hydroxy-6-keto-6-phenyl-hexa-2,4-dienoic acid 5,6 hydrolase (BphD)”, Chem. Commun., 2005, 130-132.

  29. Li, J-J, Bugg, TDH*, “A fluorescent analogue of UDP-N-acetylglucosamine: application for FRET assay of peptidoglycan translocase II (MurG)”, Chem. Commun., 2004, 182-183.

Research Interests


Students

已指导学生

包雪  硕士研究生  071010-生物化学与分子生物学  

章晶晶  硕士研究生  071010-生物化学与分子生物学  

王青  硕士研究生  071010-生物化学与分子生物学  

鲍路瑶  博士研究生  071010-生物化学与分子生物学