功能寡糖的生物合成、酶学、蛋白质工程。

硕士

1.重要功能寡糖的生物酶催化合成。

2.重要糖苷酶与糖基转移酶的酶学与蛋白质工程研究。

081703-生物化工

功能寡糖的酶催化合成，酶学，蛋白质工程

1997-09--2001-07   中国科学院化学研究所   博士

研究生学历

1997/9–2001/7， 中国科学院化学研究所， 有机化学， 博士，

1994/9–1997/9， 中国科学院福建物质结构研究所， 无机化学， 硕士，

   

2014-06~现在, 中国科学院过程工程研究所, 副研究员
2009-12~2014-05,中国科学院青岛生物能源与过程研究所, 副研究员
2007-12~2009-12,英国Illumina公司, 研发科学家
2001-11~2007-11,英国华威大学, 博士后

   

[1] 杜昱光, 李建军, 陈彤, 程功, 焦思明, 任立世. 一种海洋雪白链霉菌壳聚糖酶基因及其应用. CN: CN111154788A, 2020-05-15.

[2] 杜昱光, 李建军, 贾培媛, 任立世. 一种硫酸岩藻多糖裂解酶TFLFM及其制备方法和应用. CN: CN108531470B, 2020-04-14.

[3] 杜昱光, 荆柏林, 李建军, 王倬. 一种微流控芯片和基于该芯片的肿瘤转移模型及模型构建方法和应用. CN: CN109517737A, 2019-03-26.

[4] 杜昱光, 李建军, 任立世, 单俊伟, 王海华. 一种用于编码水解浒苔多糖的纤维素酶pJL15的核苷酸序列及其应用. 中国: CN108504666A, 2018.09.07.

   

[1] Enzyme Microb Tech. 2024,   通讯作者  
[2] RSC Adv. 2024,   通讯作者  
[3] Anal. Chem.. 2024,   通讯作者  
[4] Int. J. Biol. Macromol. 2023,   通讯作者  
[5] Anal. Chem. 2023,   通讯作者  
[6] Front. Bioeng. Biotechnol. 2023,   通讯作者  
[7] Mar. Drugs. 2022,   通讯作者  
[8] J. Infect.. 2022,   通讯作者  
[9] Bolin Jing, Kun Xia, Chen Zhang, Siming Jiao, Limeng Zhu, Jinhua Wei, Zhuo A Wang, Nannan Chen, Pengfei Tu, Jianjun Li, Yuguang Du. Chitosan Oligosaccharides Regulate the Occurrence and Development of Enteritis in a Human Gut-On-a-Chip. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY[J]. 2022, 第 10 作者10: 
[10] Yueqiang Xu, Yan Li, Xin You, Caixia Pei, Zhuo Wang, 李建军, Qi Wang, Yuguang Du. Novel insight into the sulphated glucuronic acid-based anti-SARS-CoV-2 mechanism of exopolysaccharides from halophilic archaeaon Haloarcula hispanica. Front. Chem[J]. 2022, 第 6 作者  通讯作者  
[11] Rui Yang, Mengge Gong, Siming Jiao, Zhongkai Zhou, Yuguang Du, 李建军. Protein Engineering of Pasteurella multocida 2,3-Sialyltransferase with Reduced 2,3-Sialidase Activity and Application in Synthesis of 3’-Sialyllactose. Catalysts[J]. 2022, 第 6 作者  通讯作者  
[12] 喻伟艳, 许跃强, 李建军, 李志敏, 王琪, 杜昱光, , , , , , . 新型冠状病毒的糖基化、糖受体识别及糖链抑制剂的发现. 生物工程学报[J]. 2022, 第 3 作者38(9): 3157-3172, https://cjb.ijournals.cn/cjbcn/article/abstract/gc22093157?st=article_issue.
[13] Chen, Tong, Cheng, Gong, Jiao, Siming, Ren, Lishi, Zhao, Chuanfang, Wei, Jinhua, Han, Juntian, Pei, Meishan, Du, Yuguang, Li, JianJun. Expression and Biochemical Characterization of a Novel Marine Chitosanase from Streptomyces niveus Suitable for Preparation of Chitobiose. MARINE DRUGS[J]. 2021, 第 10 作者  通讯作者  19(6): http://dx.doi.org/10.3390/md19060300.
[14] Chenlu Zhu, Zebin Liu, Lishi Ren, Zhimin Li, Yuguang Du, Li Jianjun. Overexpression and biochemical characterization of a truncated endo-α (1 → 3)-fucoidanase from alteromonas sp. SN-100. Food Chemistry[J]. 2021, 第 6 作者  通讯作者  
[15] Hua Xu, Pengfei Zhang, Yuchen Zhang, Zebin Liu, Xuebing Zhang, Zhimin Li, JianJun Li, Yuguang Du. Overexpression and Biochemical Characterization of an Endo-α-1,4-polygalacturonase from Aspergillus nidulans in Pichia pastoris. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2020, 第 7 作者21(6): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139513/.
[16] Gong, Mengge, Li, Tiechuan, Wu, Lina, Zhang, Zhenxing, Ren, Lishi, Duan, Xuexin, Cao, Hongzhi, Pei, Meishan, Li, JianJun, Du, Yuguang. Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 6 '/3 '-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System. CATALYSTS[J]. 2020, 第 11 作者10(11): http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000593655800001.
[17] Zhu, Limeng, Li, Ruilian, Jiao, Siming, Wei, Jinhua, Yan, Yalu, Wang, Zhuo A, Li, Jianjun, Du, Yuguang. Blood-Brain Barrier Permeable Chitosan Oligosaccharides Interfere with beta-Amyloid Aggregation and Alleviate beta-Amyloid Protein Mediated Neurotoxicity and Neuroinflammation in a Dose- and Degree of Polymerization-Dependent Manner. MARINE DRUGS[J]. 2020, 第 7 作者  通讯作者  18(10): https://www.webofscience.com/wos/woscc/full-record/WOS:000585114600001.
[18] Jing, Bolin, Wang, Zhuo A, Zhang, Chen, Deng, Quanfeng, Wei, Jinhua, Luo, Yong, Zhang, Xiuli, Li, Jianjun, Du, Yuguang. Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY[J]. 2020, 第 8 作者  通讯作者  8: https://doaj.org/article/821504332f9241b190608e8bee849f66.
[19] Li Jianjun. Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 60/30-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System. Catalysts. 2020, 第 1 作者  通讯作者  
[20] Limeng Zhu, Ruilian Li, Siming Jiao, Jinhua Wei, Yalu Yan, Zhuo A Wang, Jianjun Li, Yuguang Du. Blood-Brain Barrier Permeable Chitosan Oligosaccharides Interfere with β-Amyloid Aggregation and Alleviate β-Amyloid Protein Mediated Neurotoxicity and Neuroinflammation in a Dose- and Degree of Polymerization-Dependent Manner. MARINE DRUGS[J]. 2020, 第 7 作者18(10): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650801/.
[21] Liu, Dongdong, Jiao, Siming, Wei, Jinhua, Zhang, Xuebing, Pei, Yuxin, Pei, Zhichao, Li, Jianjun, Du, Yuguang. Investigation of absorption, metabolism and toxicity of ginsenosides compound K based on human organ chips. INTERNATIONAL JOURNAL OF PHARMACEUTICS[J]. 2020, 第 7 作者  通讯作者  587: http://dx.doi.org/10.1016/j.ijpharm.2020.119669.
[22] Xu, Hua, Zhang, Pengfei, Zhang, Yuchen, Liu, Zebin, Zhang, Xuebing, Li, Zhimin, Li, JianJun, Du, Yuguang. Overexpression and Biochemical Characterization of an Endo-alpha-1,4-polygalacturonase from Aspergillus nidulans in Pichia pastoris. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES[J]. 2020, 第 7 作者  通讯作者  21(6): https://www.webofscience.com/wos/woscc/full-record/WOS:000529890200196.
[23] Jing, Bolin, Luo, Yong, Lin, Bingcheng, Li, Jianjun, Wang, Zhuo A, Du, Yuguang. Establishment and application of a dynamic tumor-vessel microsystem for studying different stages of tumor metastasis and evaluating anti-tumor drugs. RSC ADVANCES[J]. 2019, 第 4 作者  通讯作者  9(30): 17137-17147, 
[24] 任立世, 程功, 焦思明, 冯翠, 张毓宸, 李建军, 杜昱光. 热紫链霉菌几丁质酶表达及低脱乙酰度壳寡糖制备. 应用与环境生物学报[J]. 2019, 第 6 作者25(2): 445-450, https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFD&dbname=CJFDLAST2019&filename=YYHS201902031&v=MjAxMDBQRFREZmJHNEg5ak1yWTlHWllSOGVYMUx1eFlTN0RoMVQzcVRyV00xRnJDVVI3dWZZK2RyRkNya1c3L08=.
[25] Jing, Bolin, Cheng, Gong, Li, Jianjun, Wang, Zhuo A, Du, Yuguang. Inhibition of Liver Tumor Cell Metastasis by Partially Acetylated Chitosan Oligosaccharide on A Tumor-Vessel Microsystem. MARINE DRUGS[J]. 2019, 第 3 作者  通讯作者  17(7): https://doaj.org/article/a8ccac9eead3471a8059abe02415ecbd.
[26] Li Jianjun. Cloning, overexpression and characterization of isomaltases IMA1 from different strains of Saccharomyces cerevisiae, Acta Microbiologica Sinica. 微生物学报. 2019, 第 1 作者  通讯作者  
[27] 程功, 焦思明, 贾培媛, 任立世, 冯翠, 张毓宸, 李建军, 杜昱光. 毕赤酵母表达环状芽胞杆菌壳聚糖酶及其水解产物组成与结构分析. 生物加工过程[J]. 2019, 第 7 作者17(1): 110-116, http://lib.cqvip.com/Qikan/Article/Detail?id=7001192198.
[28] Zhang, Pengfei, Yuan, Xianghua, Du, Yuguang, Li, JianJun. Heterologous expression and biochemica characterization of a GHF9 endoglucanase from the termite Reticulitermes speratus in Pichia pastoris. BMC BIOTECHNOLOGY[J]. 2018, 第 4 作者  通讯作者  18: https://www.webofscience.com/wos/woscc/full-record/WOS:000434342900002.
[29] Pengfei Zhang, Xianghua Yuan, Yuguang Du, JianJun Li. Heterologous expression and biochemical characterization of a GHF9 endoglucanase from the termite Reticulitermes speratus in Pichia pastoris. BMC BIOTECHNOLOGY[J]. 2018, 第 4 作者18(1): 1-9, https://doaj.org/article/75818d1b706c4fc887d6bee6d8c2e3b3.
[30] Yuan, Xianghua, Zhang, Pengfei, Jiao, Siming, Du, Yuguang, Li, JianJun. Overexpression and biochemical characterization of a recombinant psychrophilic endocellulase from Pseudoalteromonas sp DY3. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES[J]. 2018, 第 5 作者  通讯作者  116: 100-105, http://dx.doi.org/10.1016/j.ijbiomac.2018.05.017.
[31] Mao, Rui, Qi, Lifei, Li, Jianjun, Sun, Ming, Wang, Zhuo, Du, Yuguang. Competitive annealing mediated isothermal amplification of nucleic acids. ANALYST[J]. 2018, 第 3 作者143(3): 639-642, https://www.webofscience.com/wos/woscc/full-record/WOS:000423680700006.
[32] 王倬, 曾粮斌, 袁善奎, 李建军, 杜昱光. 几丁类寡糖构效关系及植物免疫诱导活性研究进展. 农药科学与管理[J]. 2018, 第 4 作者39(4): 45-50, http://lib.cqvip.com/Qikan/Article/Detail?id=675802207.
[33] 焦思明, 程功, 张毓宸, 冯翠, 任立世, 李建军, 杜昱光. 里氏木霉几丁质酶表达及其水解产物组成与结构分析*. 中国生物工程杂志[J]. 2018, 第 6 作者38(10): 30-37, https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20181004.
[34] Gao, Yu, Li, JianJun, Zheng, Lanyan, Du, Yuguang. Rational design of Pleurotus eryngii versatile ligninolytic peroxidase for enhanced pH and thermal stability through structure-based protein engineering. PROTEIN ENGINEERING DESIGN & SELECTION[J]. 2017, 第 2 作者  通讯作者  30(11): 743-751, https://www.webofscience.com/wos/woscc/full-record/WOS:000417677200001.
[35] Wang, Qing, Bao, Luyao, Jia, Chenjun, Li, Mei, Li, JianJun, Lu, Xuefeng. Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship. BMC BIOTECHNOLOGY[J]. 2017, 第 5 作者  通讯作者  17(1): http://ir.qibebt.ac.cn/handle/337004/9351.
[36] 张鹏飞, 李小连, 王自强, 袁向华, 李建军, 杜昱光. 构巢曲霉内切甘露聚糖酶在毕赤酵母中的表达及重组蛋白质的表征. 微生物学报[J]. 2017, 第 5 作者58(3): 391-400, http://ir.ipe.ac.cn/handle/122111/28864.
[37] Song, Yafeng, Fu, Gang, Dong, Huina, Li, Jianjun, Du, Yuguang, Zhang, Dawei. High-Efficiency Secretion of beta-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY[J]. 2017, 第 4 作者  通讯作者  65(12): 2540-2548, http://ir.ipe.ac.cn/handle/122111/22071.
[38] 李建军, 李小连, 王自强, 杜昱光, , , , , . 构巢曲霉内切纤维素酶A10在毕赤酵母中的表达及重组蛋白质的表征. 生物工程学报[J]. 2017, 第 1 作者33(5): 757-765, https://cjb.ijournals.cn/cjbcn/article/abstract/gc17050757?st=article_issue.
[39] Huang, Xuenian, Chen, Mei, Li, Jianjun, Lu, Xuefeng. Establishing an efficient gene-targeting system in an itaconic-acid producing Aspergillus terreus strain. BIOTECHNOLOGY LETTERS[J]. 2016, 第 3 作者38(9): 1603-1610, http://ir.qibebt.ac.cn/handle/337004/8418.
[40] Li Jianjun. Establishing an efficient gene-targeting system in an itaconic-acid producing Aspergillus terreus strain. Biotechnol. Lett.. 2016, 第 1 作者
[41] Chen, Mei, Huang, Xuenian, Zhong, Chengwei, Li, Jianjun, Lu, Xuefeng. Identification of an itaconic acid degrading pathway in itaconic acid producing Aspergillus terreus. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2016, 第 4 作者100(17): 7541-7548, http://www.irgrid.ac.cn/handle/1471x/1110090.
[42] Li Jianjun. Insight into the roles of two structural calcium ions on the properties of Pleurotus eryngii versatile ligninolytic peroxidase. Archives of Biochemistry and Biophysics. 2016, 第 1 作者  通讯作者  
[43] Li Jianjun. Identification of an itaconic acid degrading pathway in itaconic acid producing Aspergillus terreu. Appl. Microbiol. Biotechnol.. 2016, 第 1 作者
[44] Gao, Yu, Zheng, Lanyan, Li, JianJun, Du, Yuguang. Insight into the impact of two structural calcium ions on the properties of Pleurotus eryngii versatile ligninolytic peroxidase. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS[J]. 2016, 第 3 作者  通讯作者  612(DEC): 9-16, http://dx.doi.org/10.1016/j.abb.2016.10.004.
[45] Bao, Luyao, Li, JianJun, Jia, Chenjun, Li, Mei, Lu, Xuefeng. Structure-oriented substrate specificity engineering of aldehyde-deformylating oxygenase towards aldehydes carbon chain length. BIOTECHNOLOGY FOR BIOFUELS[J]. 2016, 第 2 作者  通讯作者  9(AUG): http://ir.ipe.ac.cn/handle/122111/21317.
[46] 任立世, 焦思明, 刘洪涛, 王倬, 冯翠, 贾培媛, 程功, 李建军, 未金花, 杜昱光. 岩藻寡糖的制备及抗氧化活性测定. 2015中国酶工程与糖生物工程学术研讨会论文摘要集. 2015, 第 8 作者http://ir.ipe.ac.cn/handle/122111/20431.
[47] 程功, 任立世, 焦思明, 未金花, 王倬, 贾培媛, 李建军, 刘洪涛, 冯翠, 杜昱光. 化学酶法生产可控聚合度及脱乙酰度壳寡糖方法初探. 2015中国酶工程与糖生物工程学术研讨会论文摘要集. 2015, 第 7 作者http://ir.ipe.ac.cn/handle/122111/20393.
[48] Chenjun Jia, Mei Li, Jianjun Li, Jingjing Zhang, Hongmei Zhang, Peng Cao, Xiaowei Pan, Xuefeng Lu, Wenrui Chang. Structural insights into the catalytic mechanism of aldehyde-deformylating oxygenases. PROTEIN. 2015, 第 3 作者6(1): http://kns.cnki.net/KCMS/detail/detail.aspx?QueryID=0&CurRec=8&recid=&FileName=SSJD15011600001899&DbName=SSJD_01&DbCode=SSJD&yx=&pr=&URLID=&bsm=.
[49] 李建军, 章晶晶, 王青, 鲍路瑶, 吕雪峰, 贾臣君, 李梅, 常文瑞. 脂肪醛去甲酰加氧酶（ADO）的酶学及结构-功能关系研究. 2015中国酶工程与糖生物工程学术研讨会论文摘要集. 2015, 第 1 作者http://ir.ipe.ac.cn/handle/122111/20437.
[50] 李建军, 张毓宸, 高玉, 杜昱光. 饲用组合酶的研制与开发. 2015中国酶工程与糖生物工程学术研讨会论文摘要集. 2015, 第 1 作者http://ir.ipe.ac.cn/handle/122111/20425.
[51] Huang, Xuenian, Lu, Xuefeng, Li, JianJun. Cloning, characterization and application of a glyceraldehyde-3-phosphate dehydrogenase promoter from Aspergillus terreus. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY[J]. 2014, 第 3 作者  通讯作者  41(3): 585-592, http://www.irgrid.ac.cn/handle/1471x/1001225.
[52] Li Jianjun. Engineering self-sufficient aldehyde deformylating oxygenase fused to alternative electron transfer systems for efficient conversion of aldehydes into alkanes. Chemical Communications. 2014, 第 1 作者  通讯作者  
[53] Li Jianjun. Improvement of H2O2 stability of Pleurotus eryngii versatile peroxidise through rational protein engineering. Enzyme and Microbial Technology. 2014, 第 1 作者  通讯作者  
[54] Huang, Xuenian, Chen, Mei, Lu, Xuefeng, Li, Yueming, Li, Xia, Li, JianJun. Direct production of itaconic acid from liquefied corn starch by genetically engineered Aspergillus terreus. MICROBIAL CELL FACTORIES[J]. 2014, 第 6 作者  通讯作者  13(1): http://www.irgrid.ac.cn/handle/1471x/1001226.
[55] Huang, Xuenian, Lu, Xuefeng, Li, Yueming, Li, Xia, Li, JianJun. Improving itaconic acid production through genetic engineering of an industrial Aspergillus terreus strain. MICROBIAL CELL FACTORIES[J]. 2014, 第 5 作者  通讯作者  13(1): http://www.irgrid.ac.cn/handle/1471x/1001230.
[56] Zhang, Jingjing, Lu, Xuefeng, Li, JianJun. Conversion of fatty aldehydes into alk (a/e)nes by in vitro reconstituted cyanobacterial aldehyde-deformylating oxygenase with the cognate electron transfer system. BIOTECHNOLOGY FOR BIOFUELS[J]. 2013, 第 3 作者6(1): http://www.irgrid.ac.cn/handle/1471x/1001221.
[57] Bao, Xue, Liu, Aiqiu, Lu, Xuefeng, Li, JianJun. Direct over-expression, characterization and H2O2 stability study of active Pleurotus eryngii versatile peroxidase in Escherichia coli. BIOTECHNOLOGY LETTERS[J]. 2012, 第 4 作者  通讯作者  34(8): 1537-1543, http://www.irgrid.ac.cn/handle/1471x/834355.
[58] Sergeant, Martin J, Li, JianJun, Fox, Christine, Brookbank, Nicola, Rea, Dean, Bugg, Timothy D H, Thompson, Andrew J. Selective Inhibition of Carotenoid Cleavage Dioxygenases PHENOTYPIC EFFECTS ON SHOOT BRANCHING. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2009, 第 2 作者284(8): 5257-5264, http://dx.doi.org/10.1074/jbc.M805453200.
[59] Li, JianJun, Bugg, Timothy D H. Investigation of a general base mechanism for ester hydrolysis in C-C hydrolase enzymes of the alpha/beta-hydrolase superfamily: a novel mechanism for the serine catalytic triad. ORGANIC & BIOMOLECULAR CHEMISTRY[J]. 2007, 第 1 作者5(3): 507-513, https://www.webofscience.com/wos/woscc/full-record/WOS:000243746100018.
[60] Li Jianjun. Evidence for a gem-diol reaction intermediate in bacterial C-C hydrolase enzymes BphD and MhpC from 13C NMR spectroscopy. Biochemistry. 2006, 第 1 作者
[61] Li, JJ, Bugg, TDH. A fluorescent analogue of UDP-N-acetylglucosamine: application for FRET assay of peptidoglycan translocase II (MurG). CHEMICAL COMMUNICATIONS[J]. 2004, 182-183, https://www.webofscience.com/wos/woscc/full-record/WOS:000188617000025.

   

（ 1 ） 木质素降解多样性过氧化酶H2O2稳定性与结构关系的研究, 主持, 省级, 2010-11--2013-11
（ 2 ） 衣康酸生产关键技术研究, 主持, 研究所（学校）, 2010-11--2012-11
（ 3 ） 蓝细菌脂肪醛脱羰基酶的结构-功能关系及催化机理研究, 主持, 国家级, 2012-01--2015-12
（ 4 ） 多功能木质素降解过氧化物酶的结构与H2O2 及碱稳定性关系研究, 主持, 国家级, 2014-01--2017-12
（ 5 ） 优质高产衣康酸生产平台的关键技术研究, 主持, 部委级, 2013-06--2015-12
（ 6 ） 基于多功能木质素降解过氧化物酶碱稳定性的蛋白质工程改造, 主持, 省级, 2016-01--2018-12
（ 7 ） 功能寡糖研究, 主持, 院级, 2016-02--2016-03
（ 8 ） 新型几丁寡糖免疫抗虫剂的研制, 主持, 国家级, 2017-07--2020-12
（ 9 ） 海洋壳聚糖酶制剂的研制与产品开发, 主持, 国家级, 2018-08--2021-12

已指导学生

包雪  硕士研究生  071010-生物化学与分子生物学  

章晶晶  硕士研究生  071010-生物化学与分子生物学  

王青  硕士研究生  071010-生物化学与分子生物学  

鲍路瑶  博士研究生  071010-生物化学与分子生物学