1.基于多组学的药物作用机理和靶标鉴定

虽然现代药物研发是靶标导向型的，但是仍然无法避免很高的失败风险。如果能够在临床前准确预测药物的疗效和毒副作用，就可以减少消耗，提高药物研发效率。我课题集成组化学蛋白质组学、磷酸化蛋白组学、代谢组学，结合化学生物学与生物化学研究方法，定量测定小分子药物处理细胞后引起的蛋白表达、信号通路和代谢网络中的生化变化，通过生物信息学分析，将这些变化映射到细胞过程中，从而确定小分子药物的作用靶标和作用机理。实现多维度和深层次药物作用机制的解析，从而预测治疗效果和可能出现的毒副作用。

2.高效能的药物筛选系统

我们发展了基于液相色谱、毛细管电泳以及高效液相色谱-质谱联用技术的药物筛选新技术。与传统的药物筛选技术不同，我们的方法集成了高效分离与高灵敏度检测的优势，可以直接从天然产物粗提物或者利用组合化学合成的化合物库中筛选具有重要临床治疗价值的小分子化合物。同时结合多组学药物作用机理研究，我们还在细胞层面全面评价药物的作用效果，鉴定作用靶标和脱靶效应，预测可能出现的毒副作用。

3.生物大分子间的相互作用分析

生命过程离不开蛋白-蛋白，蛋白-DNA以及蛋白-糖链之间的相互作用。研究特定蛋白质在细胞中的相互作用不仅能够阐明蛋白质的功能，揭示疾病的发病机理，还有助于发现新的治疗疾病的靶标。我们发展各种亲和色谱纯化技术结合定量蛋白组学，研究生命过程中的蛋白质相互作用和生物大分子的动态修饰。通过提高亲和纯化的效率，降低了细胞样品的复杂程度，提高了质谱数据的质量和质谱检测低丰度蛋白的灵敏度，实现无偏差的蛋白-蛋白相互作用网络的研究。我们发展的方法可以成为研究细胞生物学和生物化学的新的研究工具。

总之，我们的课题融合分析化学、化学生物学、生物化学、药物化学以及生物信息学交，学习和应用最前沿的色谱-质谱分析技术、组学技术和生物信息学技术，培养出复合型的科技人才。

   

070302-分析化学
0703Z1-化学生物学

化学蛋白组学，色谱-质谱分析，药物分析
分子药理学，药物靶标鉴定，药物筛选

1993-09--1996-12   中科院兰州化学物理研究所   理学博士
1990-09--1993-06   中科院兰州化学物理研究所   理学硕士
1986-09--1990-06   陕西师范大学化学系   学士

-- 研究生

-- 博士

   

2013-01~现在, 上海科技大学, 教授
2003-09~现在, 中科院上海有机化学研究所, 研究员
2000-10~2003-09,德国图宾根大学有机化学研究所, 博士后
1999-06~2000-10,比利时鲁汶大学药学院, 博士后

现代分离科学
现代仪器分析
色谱分离分析

   

（1） 中国发明专利奖, 三等奖, 部委级, 2019
（2） 中国分析测试协会科学技术奖, 一等奖, 部委级, 2017

（1） 乙酰胆碱酯酶抑制剂筛选方法，发明，2009，第1作者，专利号：ZL2006100276483，已实施
（2） 精对苯二酚中杂质含量的毛细管电泳内标测定，发明，2009，第1作者，专利号：ZL20061002896.9，已实施
（3） 定量分析锁链素和异锁链素的毛细管电泳胶束电动色谱方法和试剂盒，发明，2009，第1作者，专利号：ZL200610118965.6，已实施

   

（1） Chemoproteomics and Phosphoproteomics Profiling Reveals Salvianolic Acid A as a Covalent Inhibitor of mTORC1, J Proteomics Res, 2023, 通讯作者
（2） Determination of 5-methyldeoxycytosine and oxidized derivatives by nano-liquid chromatography with zwitterionic monolithic capillary column, J Chromatogr A, 2023, 通讯作者
（3） Comprehensive Profiling of Rapamycin Interacting Proteins with Multiple Mass Spectrometry-Based Omics Techniques, Anal. Chem., 2023, 通讯作者
（4） Discovery of AHCY as an Off-Target of Doxorubicin by Integrative Analysis of Photoaffinity Labeling Chemoproteomics and Untargeted Metabolomics, Anal. Chem., 2022, 通讯作者
（5） Profiling protein interactions by purification with capillary monolithic affinity column in combination with label-free quantitative proteomics, J Chromatogr A, 2022, 通讯作者
（6） Screening inhibitors for blocking UHRF1-methylated DNA interaction with capillary electrophoresis, JOURNAL OF CHROMATOGRAPHY A, 2021, 通讯作者
（7） 固定化金属离子亲和发光二氧化硅纳米粒子的制备及其用于磷酸化蛋白免疫印迹标记, Preparation of luminescent silica nanoparticles with immobilized metal ion affinity for labelling phosphorylated proteins in Western Blot, 色谱, 2021, 第 5 作者
（8） Screening inhibitors for blocking UHRF1-methylated DNA interaction with capillary electrophoresis, JOURNAL OF CHROMATOGRAPHY A, 2021, 通讯作者
（9） 固定化金属离子亲和发光二氧化硅纳米粒子的制备及其用于磷酸化蛋白免疫印迹标记, Preparation of luminescent silica nanoparticles with immobilized metal ion affinity for labelling phosphorylated proteins in Western Blot, 色谱, 2021, 第 5 作者
（10） 毛细管电泳法测定肝素和低分子量肝素平均硫酸化程度, Determination of sulfation degree of heparin and low molecular weight heparins by capillary electrophoresis, 色谱, 2020, 第 2 作者
（11） 高效毛细管电泳法测定热毒宁注射液中阴阳离子和小分子有机酸成分的含量, Determination of Zwitterion and Low-Molecular-Weight Acid in Re-Du-Ning Injection by HPCE, 世界科学技术:中医药现代化, 2020, 第 4 作者
（12） Profiling Drug-Protein Interactions by Micro Column Affinity Purification Combined with Label Free Quantification Proteomics(dagger), CHINESE JOURNAL OF CHEMISTRY, 2020, 通讯作者
（13） 离线二维色谱结合糖苷外切酶酶解用于IgG与人血清糖蛋白中N-糖链的结构表征, Structural Elucidation of N-Glycans in IgG and Human Serum Glycoprotein Through the Combination of Offline Two-dimensional Chromatography and Exoglycosidase Sequencing, 分析测试学报, 2020, 第 3 作者
（14） Preparation of Novel Zwitterionic Monolith for Capillary Electrochromatography and Nano LC-MS Applications, CHROMATOGRAPHIA, 2020, 第 4 作者
（15） Profiling Drug-Protein Interactions by Micro Column Affinity Purification Combined with Label Free Quantification Proteomics, Profiling Drug-Protein Interactions by Micro Column Affinity Purification Combined with Label Free Quantification Proteomics, 中国化学：英文版, 2020, 第 7 作者
（16） Separation of twelve posaconazole related stereoisomers by multiple heart-cutting chiral-chiral two-dimensional liquid chromatography, JOURNAL OF CHROMATOGRAPHY A, 2020, 通讯作者
（17） 胶束电动色谱柱上酶反应测定低分子量肝素的抗凝血活性, Determination of the anticoagulant activity of low molecular weight heparins by micellar electrokinetic chromatography combined with on-column enzymatic reaction, 色谱, 2020, 第 2 作者
（18） 基于化学蛋白质组学的药物靶标鉴定, Drug target deconvolution by chemical proteomics, 色谱, 2020, 第 2 作者
（19） 超快速和二维液相色谱手性分离, Chiral Separation by Ultrafast and Two-Dimensional Liquid Chromatography, 有机化学, 2020, 第 2 作者
（20） Probing Protein-Protein Interactions with Label-Free Mass Spectrometry Quantification in Combination with Affinity Purification by Spin-Tip Affinity Columns, ANALYTICAL CHEMISTRY, 2020, 通讯作者
（21） Screening of inhibitors against histone demethylation jumonji domain-containing protein 3 by capillary electrophoresis, JOURNAL OF CHROMATOGRAPHY A, 2020, 通讯作者
（22） A gold foil covered fused silica capillary tip as a sheathless interface for coupling capillary electrophoresis-mass spectrometry, JOURNAL OF CHROMATOGRAPHY A, 2020, 通讯作者
（23） 毛细管电泳法测定肝素和低分子量肝素平均硫酸化程度, Determination of sulfation degree of heparin and low molecular weight heparins by capillary electrophoresis, 色谱, 2020, 第 2 作者
（24） Profiling Drug-Protein Interactions by Micro Column Affinity Purification Combined with Label Free Quantification Proteomics(dagger), CHINESE JOURNAL OF CHEMISTRY, 2020, 通讯作者
（25） 高效毛细管电泳法测定热毒宁注射液中阴阳离子和小分子有机酸成分的含量, Determination of Zwitterion and Low-Molecular-Weight Acid in Re-Du-Ning Injection by HPCE, 世界科学技术:中医药现代化, 2020, 第 4 作者
（26） 离线二维色谱结合糖苷外切酶酶解用于IgG与人血清糖蛋白中N-糖链的结构表征, Structural Elucidation of N-Glycans in IgG and Human Serum Glycoprotein Through the Combination of Offline Two-dimensional Chromatography and Exoglycosidase Sequencing, 分析测试学报, 2020, 第 3 作者
（27） Preparation of Novel Zwitterionic Monolith for Capillary Electrochromatography and Nano LC-MS Applications, CHROMATOGRAPHIA, 2020, 第 4 作者
（28） Profiling Drug-Protein Interactions by Micro Column Affinity Purification Combined with Label Free Quantification Proteomics, Profiling Drug-Protein Interactions by Micro Column Affinity Purification Combined with Label Free Quantification Proteomics, 中国化学：英文版, 2020, 第 7 作者
（29） Separation of twelve posaconazole related stereoisomers by multiple heart-cutting chiral-chiral two-dimensional liquid chromatography, JOURNAL OF CHROMATOGRAPHY A, 2020, 通讯作者
（30） 胶束电动色谱柱上酶反应测定低分子量肝素的抗凝血活性, Determination of the anticoagulant activity of low molecular weight heparins by micellar electrokinetic chromatography combined with on-column enzymatic reaction, 色谱, 2020, 第 2 作者
（31） 基于化学蛋白质组学的药物靶标鉴定, Drug target deconvolution by chemical proteomics, 色谱, 2020, 第 2 作者
（32） 超快速和二维液相色谱手性分离, Chiral Separation by Ultrafast and Two-Dimensional Liquid Chromatography, 有机化学, 2020, 第 2 作者
（33） Probing Protein-Protein Interactions with Label-Free Mass Spectrometry Quantification in Combination with Affinity Purification by Spin-Tip Affinity Columns, ANALYTICAL CHEMISTRY, 2020, 通讯作者
（34） Screening of inhibitors against histone demethylation jumonji domain-containing protein 3 by capillary electrophoresis, JOURNAL OF CHROMATOGRAPHY A, 2020, 通讯作者
（35） A gold foil covered fused silica capillary tip as a sheathless interface for coupling capillary electrophoresis-mass spectrometry, JOURNAL OF CHROMATOGRAPHY A, 2020, 通讯作者
（36） Determining the affinity of anti-mitotic compounds binding to colchicine binding site of tubulin by affinity probe capillary electrophoresis, JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2019, 通讯作者
（37） 固定化胰蛋白酶整体小柱的制备及其用于微量蛋白质的快速酶解, Preparation of immobilized trypsin micro monolithic column and its application to rapid digestion of trace proteins, 色谱, 2019, 第 3 作者
（38） 基于共价键合法的毛细管固定化酶微反应器的制备, Enzyme assay by capillary electrophoresis with an on-column immobilized enzyme microreactor created by a covalent binding technique, 分析试验室, 2019, 第 6 作者
（39） Detection of 2,2 '-Azobis(2-amidinopropane) Dihydrochloride in Polyvinylpyrrolidone by Capillary Electrophoresis with Field-Amplified Sample Injection, CHROMATOGRAPHIA, 2019, 通讯作者
（40） Novel Triapine Derivative Induces Copper-Dependent Cell Death in Hematopoietic Cancers, JOURNAL OF MEDICINAL CHEMISTRY, 2019, 第 10 作者
（41） Determining the affinity of anti-mitotic compounds binding to colchicine binding site of tubulin by affinity probe capillary electrophoresis, JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2019, 通讯作者
（42） 固定化胰蛋白酶整体小柱的制备及其用于微量蛋白质的快速酶解, Preparation of immobilized trypsin micro monolithic column and its application to rapid digestion of trace proteins, 色谱, 2019, 第 3 作者
（43） 基于共价键合法的毛细管固定化酶微反应器的制备, Enzyme assay by capillary electrophoresis with an on-column immobilized enzyme microreactor created by a covalent binding technique, 分析试验室, 2019, 第 6 作者
（44） Detection of 2,2 '-Azobis(2-amidinopropane) Dihydrochloride in Polyvinylpyrrolidone by Capillary Electrophoresis with Field-Amplified Sample Injection, CHROMATOGRAPHIA, 2019, 通讯作者
（45） Novel Triapine Derivative Induces Copper-Dependent Cell Death in Hematopoietic Cancers, JOURNAL OF MEDICINAL CHEMISTRY, 2019, 第 10 作者
（46） Screening of break point cluster region Abelson tyrosine kinase inhibitors by capillary electrophoresis, JOURNAL OF CHROMATOGRAPHY A, 2018, 通讯作者
（47） Screening of break point cluster region Abelson tyrosine kinase inhibitors by capillary electrophoresis, JOURNAL OF CHROMATOGRAPHY A, 2018, 通讯作者
（48） Preparation of a Sulfoalkylbetaine-Based Zwitterionic Monolith with Enhanced Hydrophilicity for Capillary Electrochromatography Separation Applications, CHROMATOGRAPHIA, 2017, 第 3 作者
（49） 邻苯二甲酸为背景吸收电解质的毛细管电泳法测定低分子量肝素中的阴离子, Determination of anions in low molecular weight heparin by capillary electrophoresis with phthalate as the background electrolyte, 色谱, 2017, 第 2 作者
（50） 中药多糖及人血清中糖蛋白N-糖链的毛细管电泳指纹图谱研究, Research on Capillary Electrophoresis Fingerprints of Polysaccharides in Traditional Chinese Medicines and N-Glycome in Human Serum Glycoprotein, 分析测试学报, 2017, 第 4 作者
（51） Quantitative analysis of antithrombin III binding site in low molecular weight heparins by exhausetive heparinases digestion and capillary electrophoresis, JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2017, 通讯作者
（52） Screening of histone deacetylase 1 inhibitors in natural products by capillary electrophoresis, ANALYTICAL METHODS, 2017, 通讯作者
（53） Determination of the stereoisomeric impurities of sitafloxacin by capillary electrophoresis with dual chiral additives, JOURNAL OF CHROMATOGRAPHY A, 2017, 通讯作者
（54） Quantitative Analysis of Antithrombin III binding Site in Low Molecular Weight Heparins by Exhausetive Heparinases Digestion and Capillary Electrophoresis, JOURNAL OF CHROMATOGRAPHY B, 2017, 第 4 作者
（55） Preparation of a Sulfoalkylbetaine-Based Zwitterionic Monolith with Enhanced Hydrophilicity for Capillary Electrochromatography Separation Applications, CHROMATOGRAPHIA, 2017, 第 3 作者
（56） 邻苯二甲酸为背景吸收电解质的毛细管电泳法测定低分子量肝素中的阴离子, Determination of anions in low molecular weight heparin by capillary electrophoresis with phthalate as the background electrolyte, 色谱, 2017, 第 2 作者
（57） 中药多糖及人血清中糖蛋白N-糖链的毛细管电泳指纹图谱研究, Research on Capillary Electrophoresis Fingerprints of Polysaccharides in Traditional Chinese Medicines and N-Glycome in Human Serum Glycoprotein, 分析测试学报, 2017, 第 4 作者
（58） Quantitative analysis of antithrombin III binding site in low molecular weight heparins by exhausetive heparinases digestion and capillary electrophoresis, JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2017, 通讯作者
（59） Screening of histone deacetylase 1 inhibitors in natural products by capillary electrophoresis, ANALYTICAL METHODS, 2017, 通讯作者
（60） Determination of the stereoisomeric impurities of sitafloxacin by capillary electrophoresis with dual chiral additives, JOURNAL OF CHROMATOGRAPHY A, 2017, 通讯作者
（61） Quantitative Analysis of Antithrombin III binding Site in Low Molecular Weight Heparins by Exhausetive Heparinases Digestion and Capillary Electrophoresis, JOURNAL OF CHROMATOGRAPHY B, 2017, 第 4 作者
（62） Screening of Small-Molecule Inhibitors of Protein-Protein Interaction with Capillary Electrophoresis Frontal Analysis, ANALYTICAL CHEMISTRY, 2016, 通讯作者
（63） 高效液相色谱法手性分离二茂铁衍生物, Enantioseparation of ferroence derivatives with high performance liquid chromatography, 色谱, 2016, 第 4 作者
（64） Preparation of phosphorylcholine-based hydrophilic monolithic column and application for analysis of drug-related impurities with capillary electrochromatography, ELECTROPHORESIS, 2016, 通讯作者
（65） Screening of Small-Molecule Inhibitors of Protein-Protein Interaction with Capillary Electrophoresis Frontal Analysis, ANALYTICAL CHEMISTRY, 2016, 通讯作者
（66） 高效液相色谱法手性分离二茂铁衍生物, Enantioseparation of ferroence derivatives with high performance liquid chromatography, 色谱, 2016, 第 4 作者
（67） Preparation of phosphorylcholine-based hydrophilic monolithic column and application for analysis of drug-related impurities with capillary electrochromatography, ELECTROPHORESIS, 2016, 通讯作者
（68） Screening of mammalian target of rapamycin inhibitors in natural product extracts by capillary electrophoresis in combination with high performance liquid chromatography–tandem mass spectrometry, JOURNAL OF CHROMATOGRAPHY A, 2015, 通讯作者
（69） Screening and Identifying a Novel ssDNA Aptamer against Alpha-fetoprotein Using CE-SELEX, SCIENTIFIC REPORTS, 2015, 第 10 作者
（70） Determination of Sulfate Anions in Heparin by Capillary Electrophoresis with Improved Indirect UV Detection, CHROMATOGRAPHIA, 2015, 通讯作者
（71） Screening of epidermal growth factor receptor inhibitors in natural products by capillary electrophoresis combined with high performance liquid chromatography–tandem mass spectrometry, JOURNAL OF CHROMATOGRAPHY A, 2015, 通讯作者
（72） Screening of mammalian target of rapamycin inhibitors in natural product extracts by capillary electrophoresis in combination with high performance liquid chromatography–tandem mass spectrometry, JOURNAL OF CHROMATOGRAPHY A, 2015, 通讯作者
（73） Screening and Identifying a Novel ssDNA Aptamer against Alpha-fetoprotein Using CE-SELEX, SCIENTIFIC REPORTS, 2015, 第 10 作者
（74） Determination of Sulfate Anions in Heparin by Capillary Electrophoresis with Improved Indirect UV Detection, CHROMATOGRAPHIA, 2015, 通讯作者
（75） Screening of epidermal growth factor receptor inhibitors in natural products by capillary electrophoresis combined with high performance liquid chromatography–tandem mass spectrometry, JOURNAL OF CHROMATOGRAPHY A, 2015, 通讯作者
（76） Preparation of Durable Emitter of Electrospray Mass Spectrometry by Covalently Coating the Fused-Silica Capillary Tip with Carbon-Nanotube Sol-Gel Composite Material, CHINESE JOURNAL OF CHEMISTRY, 2014, 通讯作者
（77） Preparation of Durable Emitter of Electrospray Mass Spectrometry by Covalently Coating the Fused- Silica Capillary Tip with Carbon- Nanotube Sol-Gel Composite Material, CHIN. J. CHEM., 2014, 第 4 作者
（78） Profiling of drug binding proteins by monolithic affinity chromatography in combination with liquid chromatography-tandem mass spectrometry, J. CHROMATOGR. A, 2014, 第 6 作者
（79） Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography-tandem mass spectrometry, J. CHROMATOGR. A, 2014, 第 4 作者
（80） 伴刀豆凝集素修饰磁性纳米粒子富集人血清中糖蛋白及质谱鉴定, Enrichment of glycoproteins in human serum using concanavalin A-functionalized magnetic nanoparticles and identification by mass spectrometry, 色谱, 2014, 第 2 作者
（81） Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography–tandem mass spectrometry, JOURNAL OF CHROMATOGRAPHY A, 2014, 通讯作者
（82） Profiling of drug binding proteins by monolithic affinity chromatography in combination with liquid chromatography–tandem mass spectrometry, JOURNAL OF CHROMATOGRAPHY A, 2014, 通讯作者
（83） Preparation of Durable Emitter of Electrospray Mass Spectrometry by Covalently Coating the Fused-Silica Capillary Tip with Carbon-Nanotube Sol-Gel Composite Material, CHINESE JOURNAL OF CHEMISTRY, 2014, 通讯作者
（84） Preparation of Durable Emitter of Electrospray Mass Spectrometry by Covalently Coating the Fused- Silica Capillary Tip with Carbon- Nanotube Sol-Gel Composite Material, CHIN. J. CHEM., 2014, 第 4 作者
（85） Profiling of drug binding proteins by monolithic affinity chromatography in combination with liquid chromatography-tandem mass spectrometry, J. CHROMATOGR. A, 2014, 第 6 作者
（86） Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography-tandem mass spectrometry, J. CHROMATOGR. A, 2014, 第 4 作者
（87） 伴刀豆凝集素修饰磁性纳米粒子富集人血清中糖蛋白及质谱鉴定, Enrichment of glycoproteins in human serum using concanavalin A-functionalized magnetic nanoparticles and identification by mass spectrometry, 色谱, 2014, 第 2 作者
（88） Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography–tandem mass spectrometry, JOURNAL OF CHROMATOGRAPHY A, 2014, 通讯作者
（89） Profiling of drug binding proteins by monolithic affinity chromatography in combination with liquid chromatography–tandem mass spectrometry, JOURNAL OF CHROMATOGRAPHY A, 2014, 通讯作者
（90） 体积排阻色谱法测定低分子量肝素抗凝血因子Xa的活性, An assay for anti-factor Xa activity of low molecular weight heparins by high performance liquid size exclusion chromatography, 色谱, 2013, 第 2 作者
（91） Drug Target Identification Using Affinity Core‐Shell Magnetic Nanoparticles and Mass Spectrometry, CHINESE JOURNAL OF CHEMISTRY, 2013, 通讯作者
（92） 毛细管电泳-激光诱导荧光检测用于多个胞内蛋白激酶的抑制剂筛选和选择性评价, Inhibitor screening and selectivity assessment against multiple cellular protein kinases by capillary electrophoresis with laser-induced fluorescence detection, 色谱, 2013, 第 4 作者
（93） Structural analysis of low molecular weight heparin by ultraperformance size exclusion chromatography/time of flight mass spectrometry and capillary zone electrophoresis., ANALYTICAL CHEMISTRY, 2013, 通讯作者
（94） 毛细管电泳-激光诱导荧光检测用于多个胞内蛋白激酶的抑制剂筛选和选择性评价, Inhibitor screening and selectivity assessment against multiple cellular protein kinases by capillary electrophoresis with laser-induced fluorescence detection, 色谱, 2013, 第 4 作者
（95） 毛细管电泳结合高效液相色谱-质谱用于天然产物活性成分的筛选, Screening of the active ingredients in natural products by capillary electrophoresis and high performance liquid chromatograPhy.mass spectrometry, 色谱, 2013, 第 2 作者
（96） 体积排阻色谱法测定低分子量肝素抗凝血因子Xa的活性, An assay for anti-factor Xa activity of low molecular weight heparins by high performance liquid size exclusion chromatography, 色谱, 2013, 第 2 作者
（97） Drug Target Identification Using Affinity Core‐Shell Magnetic Nanoparticles and Mass Spectrometry, CHINESE JOURNAL OF CHEMISTRY, 2013, 通讯作者
（98） 毛细管电泳-激光诱导荧光检测用于多个胞内蛋白激酶的抑制剂筛选和选择性评价, Inhibitor screening and selectivity assessment against multiple cellular protein kinases by capillary electrophoresis with laser-induced fluorescence detection, 色谱, 2013, 第 4 作者
（99） Structural analysis of low molecular weight heparin by ultraperformance size exclusion chromatography/time of flight mass spectrometry and capillary zone electrophoresis., ANALYTICAL CHEMISTRY, 2013, 通讯作者
（100） 毛细管电泳-激光诱导荧光检测用于多个胞内蛋白激酶的抑制剂筛选和选择性评价, Inhibitor screening and selectivity assessment against multiple cellular protein kinases by capillary electrophoresis with laser-induced fluorescence detection, 色谱, 2013, 第 4 作者
（101） 毛细管电泳结合高效液相色谱-质谱用于天然产物活性成分的筛选, Screening of the active ingredients in natural products by capillary electrophoresis and high performance liquid chromatograPhy.mass spectrometry, 色谱, 2013, 第 2 作者
（102） 低分子量硫酸化多糖的体积排阻色谱法分离及其组成定量分析, Separation and quantitative analysis of the composition of low molecular weight sulfated polysaccharides by size exclusion chromatography, 色谱, 2012, 第 5 作者
（103） Self-Resistance to an Antitumor Antibiotic: A DNA Glycosylase Triggers the Base-Excision Repair System in Yatakemycin Biosynthesis, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2012, 第 7 作者
（104） 低分子量硫酸化多糖的体积排阻色谱法分离及其组成定量分析, Separation and quantitative analysis of the composition of low molecular weight sulfated polysaccharides by size exclusion chromatography, 色谱, 2012, 第 5 作者
（105） Separation and Determination of Stereoisomeric Impurity of Folinic Acid Diastereomers by CE with Vancomycin as a Selector, CHROMATOGRAPHIA, 2012, 第 3 作者
（106） Improved capillary electrophoresis frontal analysis by dynamically coating the capillary with polyelectrolyte multilayers, JOURNAL OF CHROMATOGRAPHY A, 2012, 通讯作者
（107） 低分子量硫酸化多糖的体积排阻色谱法分离及其组成定量分析, Separation and quantitative analysis of the composition of low molecular weight sulfated polysaccharides by size exclusion chromatography, 色谱, 2012, 第 5 作者
（108） Self-Resistance to an Antitumor Antibiotic: A DNA Glycosylase Triggers the Base-Excision Repair System in Yatakemycin Biosynthesis, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2012, 第 7 作者
（109） 低分子量硫酸化多糖的体积排阻色谱法分离及其组成定量分析, Separation and quantitative analysis of the composition of low molecular weight sulfated polysaccharides by size exclusion chromatography, 色谱, 2012, 第 5 作者
（110） Separation and Determination of Stereoisomeric Impurity of Folinic Acid Diastereomers by CE with Vancomycin as a Selector, CHROMATOGRAPHIA, 2012, 第 3 作者
（111） Improved capillary electrophoresis frontal analysis by dynamically coating the capillary with polyelectrolyte multilayers, JOURNAL OF CHROMATOGRAPHY A, 2012, 通讯作者
（112） A highly sensitive method for enantioseparation of fenoprofen and amino acid derivatives by capillary electrophoresis with on-line sample preconcentration, JOURNAL OF CHROMATOGRAPHY A, 2011, 通讯作者
（113） A highly sensitive method for enantioseparation of fenoprofen and amino acid derivatives by capillary electrophoresis with on-line sample preconcentration., JOURNAL OF CHROMATOGRAPHY. A, 2011, 通讯作者
（114） A highly sensitive method for enantioseparation of fenoprofen and amino acid derivatives by capillary electrophoresis with on-line sample preconcentration, JOURNAL OF CHROMATOGRAPHY A, 2011, 通讯作者
（115） A highly sensitive method for enantioseparation of fenoprofen and amino acid derivatives by capillary electrophoresis with on-line sample preconcentration., JOURNAL OF CHROMATOGRAPHY. A, 2011, 通讯作者
（116） 近期色谱研究亮点, 色谱, 2010, 第 1 作者
（117） 近期色谱研究亮点, 色谱, 2010, 第 1 作者
（118） Hexokinase inhibitor screening based on adenosine 5 '-diphosphate determination by electrophoretically mediated microanalysis, ELECTROPHORESIS, 2009, 通讯作者
（119） Determination of amino acid in formula of traditional chinese medicine using capillary electrophoresis coupled with laser-induced fluorescence detection, ACTA CHIMICA SINICA, 2009, 第 3 作者
（120） 毛细管电泳－激光诱导荧光用于中药制剂中氨基酸成分的分析, Determination of Amino Acid in Formula of Traditional Chinese Medicine Using Capillary Electrophoresis Coupled with Laser-Induced Fluorescence Detection, 化学学报, 2009, 第 3 作者
（121） Hexokinase inhibitor screening based on adenosine 5 '-diphosphate determination by electrophoretically mediated microanalysis, ELECTROPHORESIS, 2009, 通讯作者
（122） Determination of amino acid in formula of traditional chinese medicine using capillary electrophoresis coupled with laser-induced fluorescence detection, ACTA CHIMICA SINICA, 2009, 第 3 作者
（123） 毛细管电泳－激光诱导荧光用于中药制剂中氨基酸成分的分析, Determination of Amino Acid in Formula of Traditional Chinese Medicine Using Capillary Electrophoresis Coupled with Laser-Induced Fluorescence Detection, 化学学报, 2009, 第 3 作者
（124） 手性微柱高效液相色谱用于对映体的分离, Enantioseparation with A Chiral Micro-HPLC System, ACTA CHIMICA SINICA, 2008, 第 3 作者
（125） Determination of mercury ion by MEKC with on-column derivatisation and LIF detection, JOURNAL OF SEPARATION SCIENCE, 2008, 通讯作者
（126） 毛细管离子电泳法定量监测丙酮丁醇梭菌发酵过程中产生的有机酸的浓度变化, Quantitative monitoring the concentration changes of organic acids in fermentation process of Clostridium acetobutylicum using capillary ion electrophoresis, 色谱, 2008, 第 3 作者
（127） 手性微柱高效液相色谱用于对映体的分离, Enantioseparation with A Chiral Micro-HPLC System, ACTA CHIMICA SINICA, 2008, 第 3 作者
（128） 手性微柱高效液相色谱用于对映体的分离, Enantioseparation with A Chiral Micro-HPLC System, ACTA CHIMICA SINICA, 2008, 第 3 作者
（129） Determination of mercury ion by MEKC with on-column derivatisation and LIF detection, JOURNAL OF SEPARATION SCIENCE, 2008, 通讯作者
（130） 毛细管离子电泳法定量监测丙酮丁醇梭菌发酵过程中产生的有机酸的浓度变化, Quantitative monitoring the concentration changes of organic acids in fermentation process of Clostridium acetobutylicum using capillary ion electrophoresis, 色谱, 2008, 第 3 作者
（131） 手性微柱高效液相色谱用于对映体的分离, Enantioseparation with A Chiral Micro-HPLC System, ACTA CHIMICA SINICA, 2008, 第 3 作者
（132） Immobilized capillary enzyme reactor based on layer-by-layer assembling acetylcholinesterase for inhibitor screening by CE, ELECTROPHORESIS, 2007, 通讯作者
（133） 采用两性表面活性剂的部分填充胶束电动色谱作为简单的肽谱分析技术, A simple peptide mapping method by partial filling micellar electrokinetic capillary chromatography with a zwitterionic-nonionic mixed micelle AND LIFE SCIENCES, J. CHROMATOGR. B, 2007, 第 2 作者
（134） Separation and measurement of desmosine and isodesmosine in vascular tissue hydrolysates by micellar electrokinetic capillary chromatography with a mixed micelle system, JOURNAL OF CHROMATOGRAPHY A, 2007, 通讯作者
（135） A simple peptide mapping method by partial filling micellar electrokinetic capillary chromatography with a zwitterionic-nonionic mixed micelle., JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 通讯作者
（136） Immobilized capillary enzyme reactor based on layer-by-layer assembling acetylcholinesterase for inhibitor screening by CE, ELECTROPHORESIS, 2007, 通讯作者
（137） 采用两性表面活性剂的部分填充胶束电动色谱作为简单的肽谱分析技术, A simple peptide mapping method by partial filling micellar electrokinetic capillary chromatography with a zwitterionic-nonionic mixed micelle AND LIFE SCIENCES, J. CHROMATOGR. B, 2007, 第 2 作者
（138） Separation and measurement of desmosine and isodesmosine in vascular tissue hydrolysates by micellar electrokinetic capillary chromatography with a mixed micelle system, JOURNAL OF CHROMATOGRAPHY A, 2007, 通讯作者
（139） A simple peptide mapping method by partial filling micellar electrokinetic capillary chromatography with a zwitterionic-nonionic mixed micelle., JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 通讯作者
（140） Separation of atropisomers of anti-hepatitis drug dimethyl diphenyl bicarboxylate analogues by capillary electrophoresis with vancomycin as the chiral selector dirriethyl diphenyl bicarboxylate, J. CHROMATOGR. A, 2006, 第 2 作者
（141） An on-column derivatization method for the determination of the enantiomeric excess of chiral primary amines via indirect enantioseparation by micellar electrokinetic chromatography on-column derivatization, ELECTROPHORESIS, 2006, 第 2 作者
（142） Sensitive method for enantioseparation of rivastigmine with highly sulfated cyclodextrin as chiral selector by capillary electrophoresis sulfated beta-cyclodextrin, CHIN. J. CHEM., 2006, 第 4 作者
（143） Separation of atropisomers of anti-hepatitis drug dimethyl diphenyl bicarboxylate analogues by capillary electrophoresis with vancomycin as the chiral selector dirriethyl diphenyl bicarboxylate, J. CHROMATOGR. A, 2006, 第 2 作者
（144） An on-column derivatization method for the determination of the enantiomeric excess of chiral primary amines via indirect enantioseparation by micellar electrokinetic chromatography on-column derivatization, ELECTROPHORESIS, 2006, 第 2 作者
（145） Sensitive method for enantioseparation of rivastigmine with highly sulfated cyclodextrin as chiral selector by capillary electrophoresis sulfated beta-cyclodextrin, CHIN. J. CHEM., 2006, 第 4 作者
（146） 用环糊精作为手性选择剂对手性联烯酸对映体的胶束电动色谱分离, Enantioseparation of chiral allenic acids by micellar electrokinetic chromatography with cyclodextrins as chiral selector electrokinetic chromatography, ELECTROPHORESIS, 2005, 第 6 作者
（147） 用环糊精作为手性选择剂对手性联烯酸对映体的胶束电动色谱分离, Enantioseparation of chiral allenic acids by micellar electrokinetic chromatography with cyclodextrins as chiral selector electrokinetic chromatography, ELECTROPHORESIS, 2005, 第 6 作者
（148） A mechanistic study of enantiomeric separation with vancomycin and balhimycin as chiral selectors by capillary electrophoresis. Dimerization and enantioselectivity, ANAL. CHEM., 2004, 第 1 作者
（149） A mechanistic study of enantiomeric separation with vancomycin and balhimycin as chiral selectors by capillary electrophoresis. Dimerization and enantioselectivity, ANAL. CHEM., 2004, 第 1 作者

   

（ 1 ） 抗肿瘤药物蛋白激酶抑制剂的毛细管电泳筛选技术研究, 负责人, 国家任务, 2007-01--2009-12
（ 2 ） 调节细胞信号转导的靶标蛋白的鉴定新技术研究, 负责人, 国家任务, 2008-01--2010-12
（ 3 ） 亲合选择性限进色谱-质谱联用技术用于药物筛选的研究, 负责人, 国家任务, 2010-01--2012-12
（ 4 ） 重大新药创制：伊诺肝素精细结构分析, 参与, 国家任务, 2011-01--2013-12
（ 5 ） 毛细管电泳质谱联用新技术在糖链分析中的应用, 负责人, 国家任务, 2011-09--2014-12
（ 6 ） 亲合整体柱色谱用于药物靶标的鉴定, 负责人, 国家任务, 2014-01--2018-12
（ 7 ） 甲基化DNA结合蛋白的高通量鉴定和表观遗传小分子调节剂的筛选, 负责人, 国家任务, 2021-01--2023-12
（ 8 ） 基于亲合色谱和质谱分析的蛋白-蛋白相互作用识别方法研究, 负责人, 国家任务, 2018-01--2021-12
（ 9 ） Polycomb和Trithorax蛋白复合体转录调控机制的蛋白组学及小分子调 节剂筛选方法研究, 负责人, 国家任务, 2023-01--2027-12

（1）Structural Analysis of Low Molecular Weight Heparin by Ultraperformance Size Exclusion Chromatography/Time of Flight Mass Spectrometry and Capillary Zone Electrophoresis   Kang Jingwu   2014-05-21
（2）Drug target ientification by capillary affinity chromatgraphy   Kang Jingwu   2013-06-10

已指导学生

唐忠美  博士研究生  070302-分析化学  

李奕  博士研究生  070302-分析化学  

房新强  博士研究生  070302-分析化学  

程佳懿  博士研究生  070302-分析化学  

黄靖  博士研究生  070302-分析化学  

马玲  博士研究生  070302-分析化学  

汪彤丹  博士研究生  070302-分析化学  

魏云欢  博士研究生  070302-分析化学  

刘超  博士研究生  070302-分析化学  

张倩倩  博士研究生  070302-分析化学  

张含智  博士研究生  070302-分析化学  

张雪佩  博士研究生  070302-分析化学  

张艳梅  博士研究生  070302-分析化学  

李凤  博士研究生  070302-分析化学  

韩颖  硕士研究生  085216-化学工程  

张明瑜  博士研究生  070302-分析化学  

邱丹叶  博士研究生  070302-分析化学  

祝爱国  硕士研究生  070302-分析化学  

徐峰  硕士研究生  085216-化学工程  

李明霞  硕士研究生  070302-分析化学  

徐美  博士研究生  070302-分析化学  

孟然然  博士研究生  070302-分析化学  

郑蒙蒙  硕士研究生  085216-化学工程  

宫莉  博士研究生  070302-分析化学  

张梦  博士研究生  070302-分析化学  

罗阳  硕士研究生  070302-分析化学  

王子禾  硕士研究生  070302-分析化学  

沈煜婷  硕士研究生  085216-化学工程  

张益  博士研究生  070302-分析化学  

郑蒙蒙  博士研究生  070302-分析化学  

许瑶  博士研究生  070302-分析化学  

钱珊珊  博士研究生  070302-分析化学  

刘桂真  博士研究生  070302-分析化学  

丁才航  硕士研究生  070302-分析化学  

娄纯礼  博士研究生  070302-分析化学  

冯雪  硕士研究生  070302-分析化学  

叶雄珍  硕士研究生  070302-分析化学  

现指导学生

张越  博士研究生  070302-分析化学