1.基于多组学的药物作用机理和靶标鉴定

虽然现代药物研发是靶标导向型的，但是仍然无法避免很高的失败风险。如果能够在临床前准确预测药物的疗效和毒副作用，就可以减少消耗，提高药物研发效率。我课题集成组化学蛋白质组学、磷酸化蛋白组学、代谢组学，结合化学生物学与生物化学研究方法，定量测定小分子药物处理细胞后引起的蛋白表达、信号通路和代谢网络中的生化变化，通过生物信息学分析，将这些变化映射到细胞过程中，从而确定小分子药物的作用靶标和作用机理。实现多维度和深层次药物作用机制的解析，从而预测治疗效果和可能出现的毒副作用。

2.高效能的药物筛选系统

我们发展了基于液相色谱、毛细管电泳以及高效液相色谱-质谱联用技术的药物筛选新技术。与传统的药物筛选技术不同，我们的方法集成了高效分离与高灵敏度检测的优势，可以直接从天然产物粗提物或者利用组合化学合成的化合物库中筛选具有重要临床治疗价值的小分子化合物。同时结合多组学药物作用机理研究，我们还在细胞层面全面评价药物的作用效果，鉴定作用靶标和脱靶效应，预测可能出现的毒副作用。

3.生物大分子间的相互作用分析

生命过程离不开蛋白-蛋白，蛋白-DNA以及蛋白-糖链之间的相互作用。研究特定蛋白质在细胞中的相互作用不仅能够阐明蛋白质的功能，揭示疾病的发病机理，还有助于发现新的治疗疾病的靶标。我们发展各种亲和色谱纯化技术结合定量蛋白组学，研究生命过程中的蛋白质相互作用和生物大分子的动态修饰。通过提高亲和纯化的效率，降低了细胞样品的复杂程度，提高了质谱数据的质量和质谱检测低丰度蛋白的灵敏度，实现无偏差的蛋白-蛋白相互作用网络的研究。我们发展的方法可以成为研究细胞生物学和生物化学的新的研究工具。

总之，我们的课题融合分析化学、化学生物学、生物化学、药物化学以及生物信息学交，学习和应用最前沿的色谱-质谱分析技术、组学技术和生物信息学技术，培养出复合型的科技人才。

   

070302-分析化学
0703Z1-化学生物学

化学蛋白组学，色谱-质谱分析，药物分析
分子药理学，药物靶标鉴定，药物筛选

1993-09--1996-12   中科院兰州化学物理研究所   理学博士
1990-09--1993-06   中科院兰州化学物理研究所   理学硕士
1986-09--1990-06   陕西师范大学化学系   学士

-- 研究生

-- 博士

   

2013-01~现在, 上海科技大学, 教授
2003-09~现在, 中科院上海有机化学研究所, 研究员
2000-10~2003-09,德国图宾根大学有机化学研究所, 博士后
1999-06~2000-10,比利时鲁汶大学药学院, 博士后

现代分离科学
现代仪器分析
色谱分离分析

   

（1） 中国发明专利奖, 三等奖, 部委级, 2019
（2） 中国分析测试协会科学技术奖, 一等奖, 部委级, 2017

（1） 乙酰胆碱酯酶抑制剂筛选方法，发明，2009，第1作者，专利号：ZL2006100276483，已实施
（2） 精对苯二酚中杂质含量的毛细管电泳内标测定，发明，2009，第1作者，专利号：ZL20061002896.9，已实施
（3） 定量分析锁链素和异锁链素的毛细管电泳胶束电动色谱方法和试剂盒，发明，2009，第1作者，专利号：ZL200610118965.6，已实施

   

[1] J Med Chem. 2025, 第 6 作者  通讯作者  
[2] Zhang, Yue, Han, Ying, Li, Xuan, Huang, Min, Hao, Piliang, Kang, Jingwu. Ultradeep Phosphoproteomics for Assessing Protein Kinase Inhibitor Selectivity on a Proteome Scale. JOURNAL OF MEDICINAL CHEMISTRY[J]. 2025, 68(5): 5845-5855, http://dx.doi.org/10.1021/acs.jmedchem.4c03170.
[3] J Proteomics Res. 2023, 第 6 作者  通讯作者  
[4] J Chromatogr A. 2023, 第 4 作者  通讯作者  
[5] Anal. Chem.. 2023, 第 7 作者  通讯作者  
[6] Zheng, Mengmeng, Zhang, Yanmei, Xu, Yao, Han, Ying, Wu, Yingli, Kang, Jingwu. Chemoproteomics and Phosphoproteomics Profiling Reveals Salvianolic Acid A as a Covalent Inhibitor of mTORC1. JOURNAL OF PROTEOME RESEARCH[J]. 2023, 22(7): 2450-2459, http://dx.doi.org/10.1021/acs.jproteome.3c00188.
[7] Qiu, Danye, Liu, Guizhen, Li, Feng, Kang, Jingwu. Determination of 5-methyldeoxycytosine and oxidized derivatives by nano-liquid chromatography with zwitterionic monolithic capillary column. JOURNAL OF CHROMATOGRAPHY A[J]. 2023, 1693: http://dx.doi.org/10.1016/j.chroma.2023.463895.
[8] Li, Jing, Qiu, Danye, Li, Feng, Kang, Jingwu. Preparation of poly( N- vinylpyrrolidone-co-pentaerythritol triacrylate) monolithic column for hydrophilic interaction chromatography. JOURNAL OF SEPARATION SCIENCE[J]. 2023, 46(8): http://dx.doi.org/10.1002/jssc.202201033.
[9] Li, Jing, Qiu, Danye, Li, Feng, Kang, Jingwu. Preparation of poly(N-vinylpyrrolidone-co-pentaerythritol triacrylate) monolithic column for hydrophilic interaction chromatography. JOURNAL OF SEPARATION SCIENCE. 2023, 46(8): http://dx.doi.org/10.1002/jssc.202201033.
[10] jingwu.kang@sioc.ac.cn, yao xu. Comprehensive Profiling of Rapamycin Interacting Proteins with Multiple Mass Spectrometry-Based Omics Techniques. Analytical Chemistry[J]. 2023, 第 1 作者https://doi.org/10.1021/acs.analchem.3c00867.
[11] 康经武, 汪彤丹. “药物分析专辑”引言. 色谱[J]. 2023, 41(02期): 105-106, VIP_JournalArticle.
[12] 张含智, 李凤, 康经武. 毛细管电泳-无鞘流电喷雾质谱用于药物分析. 色谱[J]. 2023, 41(2): 160-167, http://lib.cqvip.com/Qikan/Article/Detail?id=7108745445.
[13] Anal. Chem.. 2022, 第 7 作者  通讯作者  
[14] J Chromatogr A. 2022, 第 6 作者  通讯作者  
[15] Qian, Shanshan, Han, Ying, Zhang, Yue, Du, Yanan, Li, Jing, Yang, Xin, Kang, Jingwu. Discovery of AHCY as an Off-Target of Doxorubicin by Integrative Analysis of Photoaffinity Labeling Chemoproteomics and Untargeted Metabolomics. ANALYTICAL CHEMISTRY. 2022, 
[16] Lou, Chunli, Ye, Xiongzhen, Chen, Ge, Zhu, Jidong, Kang, Jingwu. Screening inhibitors for blocking UHRF1-methylated DNA interaction with capillary electrophoresis. JOURNAL OF CHROMATOGRAPHY A[J]. 2021, 1636: http://dx.doi.org/10.1016/j.chroma.2020.461790.
[17] 毛雨晓, 郑蒙蒙, 刘桂真, 安保礼, 康经武. 固定化金属离子亲和发光二氧化硅纳米粒子的制备及其用于磷酸化蛋白免疫印迹标记. 色谱[J]. 2021, 39(4): 384-390, http://lib.cqvip.com/Qikan/Article/Detail?id=7104250224.
[18] 张明瑜, 康经武. 胶束电动色谱柱上酶反应测定低分子量肝素的抗凝血活性. 色谱[J]. 2020, 38(10): 1243-1248, http://lib.cqvip.com/Qikan/Article/Detail?id=7102843581.
[19] 宫莉, 康经武. 基于化学蛋白质组学的药物靶标鉴定. 色谱[J]. 2020, 38(8): 877-879, http://lib.cqvip.com/Qikan/Article/Detail?id=7102307793.
[20] 许瑶, 康经武. 超快速和二维液相色谱手性分离. 有机化学[J]. 2020, 40(11): 3794-3801, https://sioc-journal.cn/Jwk_yjhx/CN/10.6023/cjoc202005046.
[21] Liu, Guizhen, Fu, Tao, Han, Ying, Hu, Shichen, Zhang, Xuepei, Zheng, Mengmeng, Hao, Piliang, Pan, Lifeng, Kang, Jingwu. Probing Protein-Protein Interactions with Label-Free Mass Spectrometry Quantification in Combination with Affinity Purification by Spin-Tip Affinity Columns. ANALYTICAL CHEMISTRY[J]. 2020, 92(5): 3913-3922, https://www.webofscience.com/wos/woscc/full-record/WOS:000518234700057.
[22] Zhang, Yi, Lou, Chunli, Xu, Yao, Li, Jing, Qian, Shanshan, Li, Feng, Kang, Jingwu. Screening of inhibitors against histone demethylation jumonji domain-containing protein 3 by capillary electrophoresis. JOURNAL OF CHROMATOGRAPHY A[J]. 2020, 1613: http://dx.doi.org/10.1016/j.chroma.2019.460625.
[23] Gong Li, Kang Jingwu. Drug target deconvolution by chemical proteomics. CHINESE JOURNAL OF CHROMATOGRAPHY[J]. 2020, 38(8): 877-879, 
[24] Zhang, Hanzhi, Lou, Chunli, Li, Jing, Kang, Jingwu. A gold foil covered fused silica capillary tip as a sheathless interface for coupling capillary electrophoresis-mass spectrometry. JOURNAL OF CHROMATOGRAPHY A[J]. 2020, 1624: http://dx.doi.org/10.1016/j.chroma.2020.461215.
[25] 沈煜婷, 康经武. 毛细管电泳法测定肝素和低分子量肝素平均硫酸化程度. 色谱[J]. 2020, 38(10): 1238-1242, http://lib.cqvip.com/Qikan/Article/Detail?id=7102843580.
[26] Chen, Xiangyun, Zhang, Xingming, Cai, Haiyan, Yang, Wupeng, Lei, Hu, Xu, Hanzhang, Wang, Weiwei, Zhu, Qi, Kang, Jingwu, Yin, Tong, Gu, Wenli, Wu, YingLi. Targeting USP9x/SOX2 axis contributes to the anti-osteosarcoma effect of neogambogic acid. CANCER LETTERS[J]. 2020, 469: 277-286, http://dx.doi.org/10.1016/j.canlet.2019.10.015.
[27] Gong, Li, Xu, Yao, Liu, Guizhen, Zheng, Mengmeng, Zhang, Xuepei, Hang, Ying, Kang, Jingwu. Profiling Drug-Protein Interactions by Micro Column Affinity Purification Combined with Label Free Quantification Proteomics(dagger). CHINESE JOURNAL OF CHEMISTRY[J]. 2020, 38(12): 1681-1685, https://www.webofscience.com/wos/woscc/full-record/WOS:000587045600001.
[28] 葛雯, 杨彪, 于洋, 康经武, 李海波, 王振中. 高效毛细管电泳法测定热毒宁注射液中阴阳离子和小分子有机酸成分的含量＊. 世界科学技术-中医药现代化[J]. 2020, 22(11): 3913-3918, https://doi.org/10.11842/wst.20200311005.
[29] Li, Feng, Qiu, Danye, He, Jingjing, Kang, Jingwu. Preparation of Novel Zwitterionic Monolith for Capillary Electrochromatography and Nano LC-MS Applications. CHROMATOGRAPHIA[J]. 2020, 83(1): 115-122, https://www.webofscience.com/wos/woscc/full-record/WOS:000494375400001.
[30] 李凤, 张含智, 康经武. 离线二维色谱结合糖苷外切酶酶解用于IgG与人血清糖蛋白中N-糖链的结构表征. 分析测试学报[J]. 2020, 39(2): 198-204, http://lib.cqvip.com/Qikan/Article/Detail?id=7101160075.
[31] Li Gong, Yao Xu, Guizhen Liu, Mengmeng Zheng, Xuepei Zhang, Ying Hang, Jingwu Kang. Profiling Drug-Protein Interactions by Micro Column Affinity Purification Combined with Label Free Quantification Proteomics. 中国化学：英文版[J]. 2020, 38(12): 1681-1685, http://lib.cqvip.com/Qikan/Article/Detail?id=7103704698.
[32] Xu, Feng, Xu, Yao, Liu, Guizheng, Zhang, Meng, Qiang, Shangshang, Kang, Jingwu. Separation of twelve posaconazole related stereoisomers by multiple heart-cutting chiral-chiral two-dimensional liquid chromatography. JOURNAL OF CHROMATOGRAPHY A[J]. 2020, 1618: http://dx.doi.org/10.1016/j.chroma.2019.460845.
[33] Xu, Mei, Xu, Yao, Shen, Yuting, Lou, Chunli, Zheng, Mengmeng, Kang, Jingwu. Determining the affinity of anti-mitotic compounds binding to colchicine binding site of tubulin by affinity probe capillary electrophoresis. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES[J]. 2019, 1121: 66-71, http://dx.doi.org/10.1016/j.jchromb.2019.05.010.
[34] 郑蒙蒙, 韩颖, 康经武. 固定化胰蛋白酶整体小柱的制备及其用于微量蛋白质的快速酶解. 色谱[J]. 2019, 37(12): 1282-1290, https://nxgp.cnki.net/kcms/detail?v=3uoqIhG8C44YLTlOAiTRKibYlV5Vjs7iLik5jEcCI09uHa3oBxtWoNOOqu-7MuzsVbxTMSGZsYB3wOVLf5D2U93kQ1M7Mqe0&uniplatform=NZKPT.
[35] Zhang, Meng, Gong, Li, Liu, Guizhen, Kang, Jingwu. Preparation of a monolithic column with a mixed-mode stationary phase of reversed-phase/hydrophilic interaction for capillary liquid chromatography. JOURNAL OF SEPARATION SCIENCE[J]. 2019, 42(3): 662-669, https://www.webofscience.com/wos/woscc/full-record/WOS:000457651000003.
[36] 张艳梅, 李凤, 赵晓燕, 赵志勇, 周昌艳, 康经武. 基于共价键合法的毛细管固定化酶微反应器的制备. 分析试验室[J]. 2019, 434-437, http://lib.cqvip.com/Qikan/Article/Detail?id=70888389504849574852484948.
[37] Lou, Chunli, Zheng, Mengmeng, Xu, Yao, Shen, Yuting, Kang, Jingwu. Detection of 2,2 '-Azobis(2-amidinopropane) Dihydrochloride in Polyvinylpyrrolidone by Capillary Electrophoresis with Field-Amplified Sample Injection. CHROMATOGRAPHIA[J]. 2019, 82(10): 1579-1583, https://www.webofscience.com/wos/woscc/full-record/WOS:000485313700014.
[38] Yu, Jing, Liu, Zaizhou, Liang, Yuanyuan, Luo, Feng, Zhang, Jie, Tian, Cuiping, Motzik, Alex, Zheng, Mengmeng, Kang, Jingwu, Zhong, Guisheng, Liu, Cong, Fang, Pengfei, Guo, Min, Razin, Ehud, Wang, Jing. Second messenger Ap(4)A polymerizes target protein HINT1 to transduce signals in Fc epsilon RI-activated mast cells. NATURE COMMUNICATIONS[J]. 2019, 10: 
[39] Jing Yu, Zaizhou Liu, Yuanyuan Liang, Feng Luo, Jie Zhang, Cuiping Tian, Alex Motzik, Mengmeng Zheng, Jingwu Kang, Guisheng Zhong, Cong Liu, Pengfei Fang, Min Guo, Ehud Razin, Jing Wang. Second messenger Ap4A polymerizes target protein HINT1 to transduce signals in FcεRI-activated mast cells. NATURE COMMUNICATIONS[J]. 2019, 10(1): 1-12, http://dx.doi.org/10.1038/s41467-019-12710-8.
[40] Zheng Mengmeng, Han Ying, Kang Jingwu. Preparation of immobilized trypsin micro monolithic column and its application to rapid digestion of trace proteins. CHINESE JOURNAL OF CHROMATOGRAPHY[J]. 2019, 37(12): 1282-1290, 
[41] Chen, Ge, Niu, Chunyi, Yi, Jianhua, Sun, Lin, Cao, Hengyi, Fang, Yanjia, Jin, Taijie, Li, Ying, Lou, Chunli, Kang, Jingwu, Wei, Wanguo, Zhu, Jidong. Novel Triapine Derivative Induces Copper-Dependent Cell Death in Hematopoietic Cancers. JOURNAL OF MEDICINAL CHEMISTRY[J]. 2019, 62(6): 3107-3121, 
[42] Xu, Mei, Zheng, Mengmeng, Liu, Guizhen, Zhang, Meng, Kang, Jingwu. Screening of break point cluster region Abelson tyrosine kinase inhibitors by capillary electrophoresis. JOURNAL OF CHROMATOGRAPHY A[J]. 2018, 1537: 128-134, http://dx.doi.org/10.1016/j.chroma.2018.01.019.
[43] Li, Feng, Qiu, Danye, Kang, Jingwu. Preparation of a Sulfoalkylbetaine-Based Zwitterionic Monolith with Enhanced Hydrophilicity for Capillary Electrochromatography Separation Applications. CHROMATOGRAPHIA[J]. 2017, 80(6): 975-981, https://www.webofscience.com/wos/woscc/full-record/WOS:000402824400016.
[44] 张明瑜, 康经武. 邻苯二甲酸为背景吸收电解质的毛细管电泳法测定低分子量肝素中的阴离子. 色谱[J]. 2017, 35(1): 59-64, http://lib.cqvip.com/Qikan/Article/Detail?id=670940743.
[45] 李凤, 管月清, 张艳梅, 康经武. 中药多糖及人血清中糖蛋白N-糖链的毛细管电泳指纹图谱研究. 分析测试学报[J]. 2017, 36(12): 1516-1521, http://lib.cqvip.com/Qikan/Article/Detail?id=674002452.
[46] Zhang, Mingyu, Li, Gong, Zhang, Yi, Kang, Jingwu. Quantitative analysis of antithrombin III binding site in low molecular weight heparins by exhausetive heparinases digestion and capillary electrophoresis. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES[J]. 2017, 1068: 78-83, http://dx.doi.org/10.1016/j.jchromb.2017.08.047.
[47] Zhang, Yanmei, Li, Feng, Kang, Jingwu. Screening of histone deacetylase 1 inhibitors in natural products by capillary electrophoresis. ANALYTICAL METHODS[J]. 2017, 9(37): 5502-5508, https://www.webofscience.com/wos/woscc/full-record/WOS:000412072300011.
[48] Meng, Ranran, Kang, Jingwu. Determination of the stereoisomeric impurities of sitafloxacin by capillary electrophoresis with dual chiral additives. JOURNAL OF CHROMATOGRAPHY A[J]. 2017, 1506: 120-127, http://dx.doi.org/10.1016/j.chroma.2017.05.010.
[49] Zhang Mingyu, Li Gong, Zhang Yi, Kang Jingwu. Quantitative Analysis of Antithrombin III binding Site in Low Molecular Weight Heparins by Exhausetive Heparinases Digestion and Capillary Electrophoresis. JOURNAL OF CHROMATOGRAPHY B[J]. 2017, 1068-1069: 78-83, http://dx.doi.org/10.1016/j.jchromb.2017.08.047.
[50] Xu, Mei, Liu, Chao, Zhou, Mi, Li, Qing, Wang, Renxiao, Kang, Jingwu. Screening of Small-Molecule Inhibitors of Protein-Protein Interaction with Capillary Electrophoresis Frontal Analysis. ANALYTICAL CHEMISTRY[J]. 2016, 88(16): 8050-8057, http://dx.doi.org/10.1021/acs.analchem.6b01430.
[51] 徐峰, 万晓龙, 王军锋, 康经武. 高效液相色谱法手性分离二茂铁衍生物. 色谱[J]. 2016, 34(1): 57-61, http://lib.cqvip.com/Qikan/Article/Detail?id=667760182.
[52] Qiu, Danye, Li, Feng, Zhang, Mingyu, Kang, Jingwu. Preparation of phosphorylcholine-based hydrophilic monolithic column and application for analysis of drug-related impurities with capillary electrochromatography. ELECTROPHORESIS[J]. 2016, 37(12): 1725-1732, https://www.webofscience.com/wos/woscc/full-record/WOS:000379132300015.
[53] Zhang, Yanmei, Li, Feng, Li, Mingxia, Kang, Jingwu. Screening of mammalian target of rapamycin inhibitors in natural product extracts by capillary electrophoresis in combination with high performance liquid chromatography-tandem mass spectrometry. JOURNAL OF CHROMATOGRAPHY A[J]. 2015, 1388: 267-273, https://www.webofscience.com/wos/woscc/full-record/WOS:000351792400031.
[54] Dong, Lili, Tan, Qiwen, Ye, Wei, Liu, Dongli, Chen, Haifeng, Hu, Hongwei, Wen, Duo, Liu, Yang, Cao, Ya, Kang, Jingwu, Fan, Jia, Guo, Wei, Wu, Weizhong. Screening and Identifying a Novel ssDNA Aptamer against Alpha-fetoprotein Using CE-SELEX. SCIENTIFIC REPORTS[J]. 2015, 5: http://dx.doi.org/10.1038/srep15552.
[55] Zhang, Mingyu, Qiu, Danye, Kang, Jingwu. Determination of Sulfate Anions in Heparin by Capillary Electrophoresis with Improved Indirect UV Detection. CHROMATOGRAPHIA[J]. 2015, 78(11-12): 833-837, https://www.webofscience.com/wos/woscc/full-record/WOS:000354904100010.
[56] Li, Feng, Zhang, Yanmei, Qiu, DanYe, Kang, Jingwu. Screening of epidermal growth factor receptor inhibitors in natural products by capillary electrophoresis combined with high performance liquid chromatography-tandem mass spectrometry. JOURNAL OF CHROMATOGRAPHY A[J]. 2015, 1400: 117-123, http://dx.doi.org/10.1016/j.chroma.2015.04.055.
[57] Zhang, Hanzhi, Liu, Chao, Zhang, Xuepei, Kang, Jingwu. Preparation of Durable Emitter of Electrospray Mass Spectrometry by Covalently Coating the Fused- Silica Capillary Tip with Carbon- Nanotube Sol-Gel Composite Material. CHINESE JOURNAL OF CHEMISTRY[J]. 2014, 32(4): 293-297, https://www.webofscience.com/wos/woscc/full-record/WOS:000335012800001.
[58] 张含智, 刘超, 张雪佩, 康经武. Preparation of Durable Emitter of Electrospray Mass Spectrometry by Covalently Coating the Fused- Silica Capillary Tip with Carbon- Nanotube Sol-Gel Composite Material. CHIN. J. CHEM.[J]. 2014, 32(4): 293-297, http://ir.sioc.ac.cn/handle/331003/38587.
[59] Zhang Xuepei, Wang Tongdan, Zhang Hanzhi, Han Bing, Wang Lishun, Kang Jingwu. Profiling of drug binding proteins by monolithic affinity chromatography in combination with liquid chromatography-tandem mass spectrometry. J. CHROMATOGR. A[J]. 2014, 1359: 84-90, http://ir.sioc.ac.cn/handle/331003/38588.
[60] Wang Tongdan, Zhang Qianqian, Zhang Yanmei, Kang Jingwu. Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography-tandem mass spectrometry. J. CHROMATOGR. A[J]. 2014, 1337: 188-193, http://ir.sioc.ac.cn/handle/331003/38586.
[61] 李凤, 康经武. 伴刀豆凝集素修饰磁性纳米粒子富集人血清中糖蛋白及质谱鉴定. 色谱[J]. 2014, 32(4): 369-375, http://lib.cqvip.com/Qikan/Article/Detail?id=49198892.
[62] Wang, Tongdan, Zhang, Qianqian, Zhang, Yanmei, Kang, Jingwu. Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography-tandem mass spectrometry. JOURNAL OF CHROMATOGRAPHY A[J]. 2014, 1337: 188-193, http://dx.doi.org/10.1016/j.chroma.2014.02.039.
[63] Zhang, Xuepei, Wang, Tongdan, Zhang, Hanzhi, Han, Bing, Wang, Lishun, Kang, Jingwu. Profiling of drug binding proteins by monolithic affinity chromatography in combination with liquid chromatography-tandem mass spectrometry. JOURNAL OF CHROMATOGRAPHY A[J]. 2014, 1359: 84-90, http://dx.doi.org/10.1016/j.chroma.2014.07.020.
[64] 张倩倩, 康经武. 体积排阻色谱法测定低分子量肝素抗凝血因子Xa的活性. 色谱[J]. 2013, 31(7): 684-690, http://lib.cqvip.com/Qikan/Article/Detail?id=46449305.
[65] Wei, Yunhuan, Wang, Tongdan, Liu, Chao, Zhang, Qianqian, Wang, Lishun, Tang, Gongli, Kang, Jingwu. Drug Target Identification Using Affinity Core-Shell Magnetic Nanoparticles and Mass Spectrometry. CHINESE JOURNAL OF CHEMISTRY[J]. 2013, 31(6): 715-720, https://www.webofscience.com/wos/woscc/full-record/WOS:000320392400003.
[66] Zhang Qianqian, Zhang Xuepei, Zhang Hanzhi, Kang Jingwu. 毛细管电泳-激光诱导荧光检测用于多个胞内蛋白激酶的抑制剂筛选和选择性评价. 色谱[J]. 2013, 31(7): 646-655, http://lib.cqvip.com/Qikan/Article/Detail?id=46449298.
[67] 张倩倩, 张雪佩, 张含智, 康经武. 毛细管电泳-激光诱导荧光检测用于多个胞内蛋白激酶的抑制剂筛选和选择性评价. 色谱[J]. 2013, 31(7): 646-655, http://lib.cqvip.com/Qikan/Article/Detail?id=46449298.
[68] Zhang, Qianqian, Chen, Xi, Zhu, Zhijia, Zhan, Xueqiang, Wu, Yanfang, Song, Lankun, Kang, Jingwu. Structural Analysis of Low Molecular Weight Heparin by Ultraperformance Size Exclusion Chromatography/Time of Flight Mass Spectrometry and Capillary Zone Electrophoresis. ANALYTICAL CHEMISTRY[J]. 2013, 85(3): 1819-1827, http://dx.doi.org/10.1021/ac303185w.
[69] 张艳梅, 康经武. 毛细管电泳结合高效液相色谱-质谱用于天然产物活性成分的筛选. 色谱[J]. 2013, 31(7): 640-645, http://lib.cqvip.com/Qikan/Article/Detail?id=46449297.
[70] 吴艳芳1, 李晓鸽2, 车铁军3, 朱智甲1, 康经武2. 低分子量硫酸化多糖的体积排阻色谱法分离及其组成定量分析. 色谱[J]. 2012, 30(2): 173-183, https://www.chrom-china.com/CN/10.3724/SP.J.1123.2011.10005.
[71] Xu, Hui, Huang, Wei, He, QingLi, Zhao, ZhiXiong, Zhang, Feng, Wang, Renxiao, Kang, Jingwu, Tang, GongLi. Self-Resistance to an Antitumor Antibiotic: A DNA Glycosylase Triggers the Base-Excision Repair System in Yatakemycin Biosynthesis. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION[J]. 2012, 51(42): 10532-10536, http://www.irgrid.ac.cn/handle/1471x/681947.
[72] 吴艳芳, 李晓鸽, 车铁军, 朱智甲, 康经武. 低分子量硫酸化多糖的体积排阻色谱法分离及其组成定量分析. 色谱[J]. 2012, 30(2): 178-183, http://ir.sioc.ac.cn/handle/331003/28651.
[73] Wang, Zhaoyan, Mu, Changjun, Kang, Jingwu, Hu, Zhide. Separation and Determination of Stereoisomeric Impurity of Folinic Acid Diastereomers by CE with Vancomycin as a Selector. CHROMATOGRAPHIA[J]. 2012, 75(19-20): 1211-1215, https://www.webofscience.com/wos/woscc/full-record/WOS:000309223800015.
[74] Liu, Chao, Kang, Jingwu. Improved capillary electrophoresis frontal analysis by dynamically coating the capillary with polyelectrolyte multilayers. JOURNAL OF CHROMATOGRAPHY A[J]. 2012, 1238: 146-151, http://dx.doi.org/10.1016/j.chroma.2012.03.043.
[75] Wang, Zhaoyan, Liu, Chao, Kang, Jingwu. A highly sensitive method for enantioseparation of fenoprofen and amino acid derivatives by capillary electrophoresis with on-line sample preconcentration. JOURNAL OF CHROMATOGRAPHY A[J]. 2011, 1218(13): 1775-1779, http://dx.doi.org/10.1016/j.chroma.2011.01.081.
[76] Wang Zhaoyan, Liu Chao, Kang Jingwu. A highly sensitive method for enantioseparation of fenoprofen and amino acid derivatives by capillary electrophoresis with on-line sample preconcentration.. JOURNAL OF CHROMATOGRAPHY. A[J]. 2011, 1775-1779, http://dx.doi.org/10.1016/j.chroma.2011.01.081.
[77] 康经武. 近期色谱研究亮点. 色谱[J]. 2010, 28(3): 223-224, http://lib.cqvip.com/Qikan/Article/Detail?id=33204024.
[78] Wang, Tongdan, Kang, Jingwu. Hexokinase inhibitor screening based on adenosine 5 '-diphosphate determination by electrophoretically mediated microanalysis. ELECTROPHORESIS[J]. 2009, 30(8): 1349-1354, http://www.irgrid.ac.cn/handle/1471x/681875.
[79] Ding Juanjuan, Jin Baokang, Kang Jingwu. Determination of Amino Acid in Formula of Traditional Chinese Medicine Using Capillary Electrophoresis Coupled with Laser-Induced Fluorescence Detection. ACTA CHIMICA SINICA[J]. 2009, 67(9): 945-950, http://www.corc.org.cn/handle/1471x/2159717.
[80] 丁娟娟, 金葆康, 康经武. 毛细管电泳－激光诱导荧光用于中药制剂中氨基酸成分的分析. 化学学报[J]. 2009, 945-950, http://lib.cqvip.com/Qikan/Article/Detail?id=30466050.
[81] Zhu ZhiJia, Wang QianQian, Kang JingWu. Enantioseparation with a chiral micro-HPLC system. ACTA CHIMICA SINICA[J]. 2008, 66(15): 1845-1849, https://www.webofscience.com/wos/woscc/full-record/WOS:000258730500013.
[82] Ma, Ling, Kang, Jingwu. Determination of mercury ion by MEKC with on-column derivatisation and LIF detection. JOURNAL OF SEPARATION SCIENCE[J]. 2008, 31(5): 888-892, http://dx.doi.org/10.1002/jssc.200700606.
[83] 程佳懿, 汪彤丹, 康经武. 毛细管离子电泳法定量监测丙酮丁醇梭菌发酵过程中产生的有机酸的浓度变化. 色谱[J]. 2008, 26(6): 658-661, http://lib.cqvip.com/Qikan/Article/Detail?id=28861286.
[84] Zhu ZhiJia, Wang QianQian, Kang JingWu. Enantioseparation with a chiral micro-HPLC system. ACTA CHIMICA SINICA[J]. 2008, 66(15): 1845-1849, https://www.webofscience.com/wos/woscc/full-record/WOS:000258730500013.
[85] Tang, Zhongmei, Wang, Tongdan, Kang, Jingwu. Immobilized capillary enzyme reactor based on layer-by-layer assembling acetylcholinesterase for inhibitor screening by CE. ELECTROPHORESIS[J]. 2007, 28(17): 2981-2987, http://www.irgrid.ac.cn/handle/1471x/670656.
[86] 黄靖, 康经武. A simple peptide mapping method by partial filling micellar electrokinetic capillary chromatography with a zwitterionic-nonionic mixed micelle AND LIFE SCIENCES. J. CHROMATOGR. B[J]. 2007, 846(1-2): 364-367, http://ir.sioc.ac.cn/handle/331003/27687.
[87] Huang, Jing, Kang, Jingwu. Separation and measurement of desmosine and isodesmosine in vascular tissue hydrolysates by micellar electrokinetic capillary chromatography with a mixed micelle system. JOURNAL OF CHROMATOGRAPHY A[J]. 2007, 1175(2): 294-296, http://dx.doi.org/10.1016/j.chroma.2007.10.091.
[88] Huang, Jing, Kang, Jingwu. A simple peptide mapping method by partial filling micellar electrokinetic capillary chromatography with a zwitterionic-nonionic mixed micelle. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES[J]. 2007, 846(1-2): 364-367, http://dx.doi.org/10.1016/j.jchromb.2006.08.035.
[89] 高文华, 康经武. Separation of atropisomers of anti-hepatitis drug dimethyl diphenyl bicarboxylate analogues by capillary electrophoresis with vancomycin as the chiral selector dirriethyl diphenyl bicarboxylate. J. CHROMATOGR. A[J]. 2006, 1108(1): 145-148, http://ir.sioc.ac.cn/handle/331003/27649.
[90] 王兆彦, 许杏祥, 胡之德, 康经武. Sensitive method for enantioseparation of rivastigmine with highly sulfated cyclodextrin as chiral selector by capillary electrophoresis sulfated beta-cyclodextrin. CHIN. J. CHEM.[J]. 2006, 24(10): 1384-1387, http://ir.sioc.ac.cn/handle/331003/24357.
[91] 程佳懿, 康经武. An on-column derivatization method for the determination of the enantiomeric excess of chiral primary amines via indirect enantioseparation by micellar electrokinetic chromatography on-column derivatization. ELECTROPHORESIS[J]. 2006, 27(4): 865-871, http://ir.sioc.ac.cn/handle/331003/27647.
[92] 王兆彦, 唐忠美, 顾振华, 胡之德, 麻生明, 康经武. Enantioseparation of chiral allenic acids by micellar electrokinetic chromatography with cyclodextrins as chiral selector electrokinetic chromatography. ELECTROPHORESIS[J]. 2005, 26(4-5): 1001-1006, http://ir.sioc.ac.cn/handle/331003/12028.
[93] Kang Jingwu, Daniel Bischoff, Jiang Zhengjin, Bojan Bister, Roderich D. Süssmuth, Volker Schurig. A mechanistic study of enantiomeric separation with vancomycin and balhimycin as chiral selectors by capillary electrophoresis. Dimerization and enantioselectivity. ANAL. CHEM.[J]. 2004, 76(8): 2387-2392, http://www.irgrid.ac.cn/handle/1471x/670614.
[94] Kang, JW, Wistuba, D, Schurig, V. Fast enantiomeric separation with vancomycin as chiral additive by co-electroosmotic flow capillary electrophoresis: Increase of the detection sensitivity by the partial filling technique. ELECTROPHORESIS[J]. 2003, 24(15): 2674-2679, https://www.webofscience.com/wos/woscc/full-record/WOS:000184932400032.
[95] Kang, JW, Wistuba, D, Schurig, V. Recent progress in enantiomeric separation by capillary electrochromatography. ELECTROPHORESIS. 2002, 23(22-23): 4005-4021, 
[96] Kang, JW, Wistuba, D, Schurig, V. A silica monolithic column prepared by the sol-gel process for enantiomeric separation by capillary electrochromatography. ELECTROPHORESIS[J]. 2002, 23(7-8): 1116-1120, 
[97] Kang, JW, De Reymaeker, G, Van Schepdael, A, Roets, E, Hoogmartens, J. Analysis of bacitracin by micellar electrokinetic capillary chromatography with mixed micelle in acidic solution. ELECTROPHORESIS[J]. 2001, 22(7): 1356-1362, https://www.webofscience.com/wos/woscc/full-record/WOS:000168694800014.
[98] Kang, JW, Van Schepdael, A, Roets, E, Hoogmartens, J. Analysis of vancomycin and related impurities by micellar electrokinetic capillary chromatography. Method development and validation. ELECTROPHORESIS[J]. 2001, 22(12): 2588-2592, https://www.webofscience.com/wos/woscc/full-record/WOS:000170529800027.
[99] Lu, HJ, Ruan, ZQ, Kang, JW, Ou, QY. Simultaneous chiral separation and determination of the optical purity of naproxen and methyl naproxen by capillary electrophoresis with dual-cyclodextrin system as chiral selector. ANALYTICAL LETTERS[J]. 2001, 34(10): 1657-1668, https://www.webofscience.com/wos/woscc/full-record/WOS:000170769800007.
[100] Kang, JW, Van Schepdael, A, Orwa, JA, Roets, E, Hoogmartens, J. Analysis of polymyxin B sulfate by capillary zone electrophoresis with cyclodextrin as additive Method development and validation. JOURNAL OF CHROMATOGRAPHY A[J]. 2000, 879(2): 211-218, http://dx.doi.org/10.1016/S0021-9673(00)00343-5.
[101] Kang, JW, Vankeirsbilck, T, Van Schepdael, A, Orwa, J, Roets, E, Hoogmartens, J. Analysis of colistin sulfate by capillary zone electrophoresis with cyclodextrins as additive. ELECTROPHORESIS[J]. 2000, 21(15): 3199-3204, https://www.webofscience.com/wos/woscc/full-record/WOS:000089318300018.
[102] Lu, HJ, Ruan, ZQ, Kang, JW, Ou, QY. Chiral separation of naproxen and methyl naproxen ester by capillary electrophoresis. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY[J]. 2000, 28(9): 1057-1061, https://www.webofscience.com/wos/woscc/full-record/WOS:000089558200001.
[103] Yang, YT, Liu, FJ, Kang, JW, Ou, QY. Improved selectivity of anions with methanol as additive determination of Cl-, NO3- and SO42- in river water by capillary electrophoresis. JOURNAL OF CHROMATOGRAPHY A[J]. 1999, 834(1-2): 393-399, http://dx.doi.org/10.1016/S0021-9673(98)00859-0.
[104] Yang, YT, Kang, JW, Lu, HJ, Ou, QY, Liu, FJ. Determination of trace level anions in snow samples by capillary electrophoresis with sample stacking. JOURNAL OF CHROMATOGRAPHY A[J]. 1999, 834(1-2): 387-391, http://dx.doi.org/10.1016/S0021-9673(98)00862-0.
[105] Kang, JW, Ou, QY. Chiral separation of racemic mexiletine hydrochloride using cyclodextrins as chiral additive by capillary electrophoresis. JOURNAL OF CHROMATOGRAPHY A[J]. 1998, 795(2): 394-398, http://dx.doi.org/10.1016/S0021-9673(97)00974-6.
[106] Fan, XJ, You, JM, Kang, JW, Ou, QY, Zhu, QC. New reagents for determination of amino acids by liquid chromatography with pre-column fluorescence derivatization. ANALYTICA CHIMICA ACTA[J]. 1998, 367(1-3): 81-91, http://dx.doi.org/10.1016/S0003-2670(98)00125-1.
[107] Kang, JW, Yang, YT, You, JM, Ou, QY. Fast chiral separation of amino acid derivatives and acidic drugs by co-electroosmotic flow capillary electrophoresis with vancomycin as chiral selector. JOURNAL OF CHROMATOGRAPHY A[J]. 1998, 825(1): 81-87, http://dx.doi.org/10.1016/S0021-9673(98)00679-7.
[108] Yang, YT, Kang, JW, You, JM, Ou, QY. Improved separation and determination of rare earth metal ions in real samples by capillary electrophoresis with indirect UV detection. ANALYTICAL LETTERS[J]. 1998, 31(11): 1955-1964, https://www.webofscience.com/wos/woscc/full-record/WOS:000075679000014.
[109] 康经武, 孙增培, 俞惟乐, 欧庆瑜, 阮宗琴. 盐酸美西律对映体的毛细管电泳分离. 分析化学[J]. 1997, 25(3): 290, http://lib.cqvip.com/Qikan/Article/Detail?id=2659769.
[110] 康经武, 欧庆瑜. 二烯丙基聚乙二醇20M交联键合型毛细管电泳柱的制备及其评价. 分析化学[J]. 1996, 24(2): 144, http://lib.cqvip.com/Qikan/Article/Detail?id=2006688.
[111] 康经武, 江天籁. 穴醚2.2.2的HgCl↓2，CdCl↓2及Cd（NO↓3）↓2配合物的红外和拉曼光谱. 光谱学与光谱分析[J]. 1995, 15(5): 23, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=270058&detailType=1.

   

（ 1 ） 新药创制-药物靶标鉴定, 参与, 研究所自主部署, 2024-01--2025-01
（ 2 ） Polycomb和Trithorax蛋白复合体转录调控机制的蛋白组学及小分子调 节剂筛选方法研究, 负责人, 国家任务, 2023-01--2027-12
（ 3 ） 企业横向, 负责人, 其他, 2022-01--2026-01
（ 4 ） 甲基化DNA结合蛋白的高通量鉴定和表观遗传小分子调节剂的筛选, 负责人, 国家任务, 2021-01--2023-12
（ 5 ） 基于亲合色谱和质谱分析的蛋白-蛋白相互作用识别方法研究, 负责人, 国家任务, 2018-01--2021-12
（ 6 ） 亲合整体柱色谱用于药物靶标的鉴定, 负责人, 国家任务, 2014-01--2018-12
（ 7 ） 毛细管电泳质谱联用新技术在糖链分析中的应用, 负责人, 国家任务, 2011-09--2014-12
（ 8 ） 重大新药创制：伊诺肝素精细结构分析, 参与, 国家任务, 2011-01--2013-12
（ 9 ） 亲合选择性限进色谱-质谱联用技术用于药物筛选的研究, 负责人, 国家任务, 2010-01--2012-12
（ 10 ） 调节细胞信号转导的靶标蛋白的鉴定新技术研究, 负责人, 国家任务, 2008-01--2010-12
（ 11 ） 抗肿瘤药物蛋白激酶抑制剂的毛细管电泳筛选技术研究, 负责人, 国家任务, 2007-01--2009-12

（1）Structural Analysis of Low Molecular Weight Heparin by Ultraperformance Size Exclusion Chromatography/Time of Flight Mass Spectrometry and Capillary Zone Electrophoresis   Kang Jingwu   2014-05-21
（2）Drug target ientification by capillary affinity chromatgraphy   Kang Jingwu   2013-06-10

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唐忠美  博士研究生  070302-分析化学  

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房新强  博士研究生  070302-分析化学  

程佳懿  博士研究生  070302-分析化学  

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