高通量单细胞技术

微生物组

招收相关专业博士和硕士研究生

071005-微生物学
081703-生物化工
070302-分析化学

定量微生物学微流控新技术，高通量微生物筛选，便携式微生物快检
高通量工业微生物选育，发酵在线监测，单细胞技术
微流控技术, 拉曼细胞分选

2003-09--2008-05   中科院大连化物所   博士
1998-09--2001-07   四川大学   硕士
1994-09--1998-07   西华师范大学   学士

   

2010-12~2012-07,Rice University, 博士后研究员
2009-06~2010-11,Texas A&M University, 博士后研究员
2008-06~2009-06,University of Carlifornia, Los angeles, 博士后研究员
2001-07~2003-07,西华师范大学, 讲师

前沿论坛
功能基因组
单细胞功能基因组

   

[1] Jing, Xiaoyan, Gong, Yanhai, Xu, Teng, Meng, Yu, Han, Xiao, Su, Xiaolu, Wang, Jianmei, Ji, Yuetong, Li, Yuandong, Jia, Zhongjun, Ma, Bo, Xu, Jian. One-Cell Metabolic Phenotyping and Sequencing of Soil Microbiome by Raman-Activated Gravity-Driven Encapsulation (RAGE). MSYSTEMS[J]. 2021, 6(3): http://dx.doi.org/10.1128/mSystems.00181-21.
[2] Xu, Teng, Han, Xiao, Zhu, Pengfei, Dai, Jing, Liu, Min, Liu, Yang, Xu, Jian, Ma, Bo. A milliliter to picoliter-level centrifugal microfluidic concentrator for fast pathogen detection and antimicrobial susceptibility testing. SENSORS AND ACTUATORS B-CHEMICAL[J]. 2021, 343: http://dx.doi.org/10.1016/j.snb.2021.130117.
[3] Xu, Teng, Gong, Yanhai, Su, Xiaolu, Zhu, Pengfei, Dai, Jing, Xu, Jian, Ma, Bo. Phenome-Genome Profiling of Single Bacterial Cell by Raman-Activated Gravity-Driven Encapsulation and Sequencing. SMALL[J]. 2020, 16(30): https://www.webofscience.com/wos/woscc/full-record/WOS:000538997700001.
[4] Wang, Xixian, Xin, Yi, Ren, Lihui, Sun, Zheng, Zhu, Pengfei, Ji, Yuetong, Li, Chunyu, Xu, Jian, Ma, Bo. Positive dielectrophoresis-based Raman-activated droplet sorting for culture-free and label-free screening of enzyme function in vivo. SCIENCE ADVANCES[J]. 2020, 6(32): https://www.webofscience.com/wos/woscc/full-record/WOS:000560452300026.
[5] Liu, Fengyi, Xu, Teng, Liu, Wenjing, Zheng, Xiaoshan, Xu, Jian, Ma, Bo. Spontaneous droplet generation via surface wetting. LAB ON A CHIP[J]. 2020, 20(19): 3544-3551, https://www.webofscience.com/wos/woscc/full-record/WOS:000573314400002.
[6] Cui, Xiaonan, Ren, Lihui, Shan, Yufei, Wang, Xixian, Yang, Zhenlong, Li, Chunyu, Xu, Jian, Ma, Bo. Smartphone-based rapid quantification of viable bacteria by single-cell microdroplet turbidity imaging. ANALYST[J]. 2018, 143(14): 3309-3316, https://www.webofscience.com/wos/woscc/full-record/WOS:000438056400006.
[7] Wang, Xixian, Ren, Lihui, Su, Yetian, Ji, Yuetong, Liu, Yaoping, Li, Chunyu, Li, Xunrong, Zhang, Yi, Wang, Wei, Hu, Qiang, Han, Danxiang, Xu, Jian, Ma, Bo. Raman-Activated Droplet Sorting (RADS) for Label-Free High-Throughput Screening of Microalgal Single-Cells. ANALYTICAL CHEMISTRY[J]. 2017, 89(22): 12569-12577, http://dx.doi.org/10.1021/acs.analchem.7b03884.
[8] Zhang, Qiang, Wang, Tingting, Zhou, Qian, Zhang, Peng, Gong, Yanhai, Gou, Honglei, Xu, Jian, Ma, Bo. Development of a facile droplet-based single-cell isolation platform for cultivation and genomic analysis in microorganisms. SCIENTIFIC REPORTS[J]. 2017, 7: http://www.irgrid.ac.cn/handle/1471x/1755909.
[9] 马波. Reconstituting Microenvironment Simulation System for Bladder cancer Based on Microfluidic Co-culture Model. ONCOTARGET. 2015, [10] Li, Chunyu, Xu, Jian, Ma, Bo. Precise quantitative addition of multiple reagents into droplets in sequence using glass fiber-induced droplet coalescence. ANALYST[J]. 2015, 140(3): 701-705, http://ir.qibebt.ac.cn/handle/337004/6079.
[11] Zhang, Qiang, Zhang, Peiran, Gou, Honglei, Mou, Chunbo, Huang, Wei E, Yang, Menglong, Xu, Jian, Ma, Bo. Towards high-throughput microfluidic Raman-activated cell sorting. ANALYST[J]. 2015, 140(18): 6163-6174, http://www.irgrid.ac.cn/handle/1471x/1000833.
[12] Zhang, Peiran, Ren, Lihui, Zhang, Xu, Shan, Yufei, Wang, Yun, Ji, Yuetong, Yin, Huabing, Huang, Wei E, Xu, Jian, Ma, Bo. Raman-Activated Cell Sorting Based on Dielectrophoretic Single-Cell Trap and Release. ANALYTICAL CHEMISTRY[J]. 2015, 87(4): 2282-2289, http://ir.qibebt.ac.cn/handle/337004/6084.
[13] 马波. A Self-Powered Microfluidic Monodispersed Droplet Generator with Capability of Multi-Sample Introduction. Microfluidics and Nanofluidics. Microfluidics and Nanofluidics. 2014, [14] Zhang, Qiang, Zhang, Peiran, Su, Yetian, Mou, Chunbo, Zhou, Teng, Yang, Menglong, Xu, Jian, Ma, Bo. On-demand control of microfluidic flow via capillary-tuned solenoid microvalve suction. LAB ON A CHIP[J]. 2014, 14(24): 4599-4603, http://ir.qibebt.ac.cn/handle/337004/6280.
[15] 马波. Portable microfluidics device for quantitative RT-PCR. 2013, [16] Ma, Bo, Ghavim, Sima, Sutton, Richard L, Harris, Neil G, Phelps, Michael, Wu, HsiaoMing. Real time blood plasma separation in a microfluidic chip. JOURNAL OF NUCLEAR MEDICINEnull. 2009, 50: https://www.webofscience.com/wos/woscc/full-record/WOS:000444145700472.
[17] Ma, Bo, Zhang, Guohao, Qin, Jianhua, Lin, Bingcheng. Characterization of drug metabolites and cytotoxicity assay simultaneously using an integrated microfluidic device. LAB ON A CHIP[J]. 2009, 9(2): 232-238, http://159.226.238.44/handle/321008/101393.
[18] 张国豪, 马波, 秦建华, 林炳承. 集成药物代谢微流控芯片的研制. 高等学校化学学报[J]. 2008, 29(7): 1356-1358, http://lib.cqvip.com/Qikan/Article/Detail?id=27899048.
[19] Ma, Bo, Zhou, Xiaomian, Wang, Gang, Dai, Zhongpeng, Qin, Jianhua, Lin, Bingcheng. A hybrid microdevice with a thin PDMS membrane on the detection window for UV absorbance detection. ELECTROPHORESIS[J]. 2007, 28(14): 2474-2477, http://159.226.238.44/handle/321008/98697.
[20] Ma Bo, Zhou Xiaomian, Wang Gang, Huang Huaiqing, Dai Zhongpeng, Qin Jianhua, Lin Bingcheng. Integrated isotachophoretic preconcentration with zone electrophoresis separation on a quartz microchip for UV detection of flavonoids. ELECTROPHORESIS[J]. 2006, 27(24): 4904-4909, http://159.226.238.44/handle/321008/98075.
[21] 马波. 玻璃微流控芯片性能评价. 高等学校化学学报. 2005, [22] 马波. 黄酮类化合物抗肿瘤活性量子化学研究. 化学研究与应用. 2002, [23] 马波. 四川大学植物标本数据库的构建. 四川大学学报. 2001, [24] 马波, 周在德, 李梦龙. 人工神经网络及其在色谱中的应用. 化学研究与应用[J]. 2000, 12(4): 375-, http://lib.cqvip.com/Qikan/Article/Detail?id=4511449.

   

（ 1 ） 单细胞功能基因组及自动培养系统研制, 参与, 部委级, 2012-11--2014-10
（ 2 ） 用于农产品致病原及污染物现场即时检测的智能化微流控芯片系统研制, 参与, 部委级, 2013-06--2016-07
（ 3 ） 基于阵列介电捕获的微生物单细胞高通量拉曼流式分选方法学研究 , 主持, 国家级, 2015-01--2018-12
（ 4 ） 基于液滴微流控技术的快速生长雨声红球藻株高通量筛选方法研究, 主持, 国家级, 2014-06--2016-06
（ 5 ） 耦合液滴单细胞培养的快速微生物鉴定质谱接口关键技术 研究, 主持, 国家级, 2018-01--2021-12
（ 6 ） 高通量拉曼流式细胞分选仪的研制, 主持, 部委级, 2018-01--2019-12
（ 7 ） 高灵敏、高通量、多参数光学检测分析仪器研发, 主持, 国家级, 2019-07--2024-06

已指导学生

崔晓楠  硕士研究生  085238-生物工程  

张嘉萍  硕士研究生  085238-生物工程  

刁志钿  硕士研究生  085238-生物工程  

刘沣仪  硕士研究生  085238-生物工程  

徐腾  博士研究生  071005-微生物学  

现指导学生

刘沣仪  博士研究生  071010-生物化学与分子生物学  

周浩  硕士研究生  071005-微生物学