1. Biosynthetic P450 enzymes;

2. Natural product biosynthesis;

3. Protein engineering;

4. Metabolic engineering for biofuel production.

1. 09/2004-12/2009  Ph.D. in Medicinal Chemistry

Department of Medicinal Chemistry and Life Sciences Institute, University of Michigan, Ann Arbor, Michigan, USA

2. 09/2000-07/2003  M.S. in Microbiology

School of Life Sciences, Xiamen University, Fujian, China

3.  09/1996-07/2000  B.S. in Biology

School of Life Sciences, Xiamen University, Fujian, China

1. 06/2012-now        Professor, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences

Research Fields: Biosynthetic P450 enzymes; Natural product biosynthesis; Protein engineering; Metabolic engineering for biofuel production.

2. 08/2014-now        Director, Shandong Provincial Key Laboratory of Synthetic Biology

3. 01/2010-05/2012  Postdoctoral Research Fellow, Life Sciences Institute, University of Michigan

Research Fields: Fungal alkaloid biosynthesis; Biosynthetic P450 enzymes.

4.  Concurrently         Research Scientist, Alluvium Biosciences Inc.

Research Fields: Industrial strain improvement; Chemo-enzymatic synthesis of novel antibiotics.

1.      Zhang, X., Li, Z., Du, L. Chlipala, G. E., Lopez, P. C., Zhang W., Sherman, D. H. and Li, S.* Identification of an unexpected shunt pathway product provides new insights into tirandamycin biosynthesis. Tetrahedron Lett., 2016. (In press)

2.      Sun, Y., Ma, L., Han, D., Du L., Qi, F, Zhang, W., Sun, J., Huang, S., Eung-Soo Kim, and Li, S.*. In Vitro reconstitution of the cyclosporine specific P450 hydroxylases using heterologous redox partner proteins. J. Ind. Micro. Biot., 2016. (In press)

3.      Zheng, X., Fang, B., Han, D., Yang, W., Qi, F., Chen, H., and Li, S.*. Improving the Secretory Expression of an α-Galactosidase from Aspergillus niger in Pichia pastoris. (2016) PLoS ONE 11(8): e0161529. doi:10.1371/journal.pone.0161529. (IF: 3.057)

4.      Du, L., Ma, L., Qi, F., Zheng, X., Jiang, C., Li, A., Wan, X., Liu, S.-J.*, and Li, S.* Characterization of a Unique Pathway for 4-Cresol Catabolism Initiated by Phosphorylation in Corynebacterium glutamicum. J. Biol. Chem. 2016, 291, 6583-6584. (IF: 4.258)

5.      Ma, L., Du, L., Chen, H., Sun, Y., Huang, S., Zheng, X., Kim, E.-S.*, and Li, S.* Reconstitution of the in vitro activity of the cyclosporine-specific P450 hydroxylase from Sebekia benihana and development of a heterologous whole cell biotransformation system. Appl. Environ. Microbiol. 2015, 81(18), 6268-75. (IF: 3.668)

6.      Woo, M. W., Lee, B. R., Nah, H.J., Choi, S. S., Li, S., and Kim, E.-S.* Domain Characterization of Cyclosporin Regio-specific Hydroxylases in Rare Actinomycetes. J. Microbiol. Biotechnol. 2015, 25(10), 1634-1639. (IF: 1.32)

7.      Qi, G., Wang, D., Yu, L., Tang, X., Chai, G., He, G., Ma, W., Li, S., Kong, Y., Fu, C., and Zhou, G.* Metabolic engineering of 2-phenylethanol pathway producing fragrance chemical and reducing lignin in Arabidopsis. Plant Cell Rep. 2015, 34(8), 1331-1342. (IF: 3.071) 

8.      Bernard, S. M., Akey, D. L., Tripathi, A., Park, S. R., Konwerski, J. R., Anzai, Y., Li, S., Kato, F., Sherman, D. H., and Smith, J. L.* Structural Basis of Substrate Specificity and Regiochemistry in the MycF/TylF Family of Sugar O-Methyltransferases. ACS Chem. Biol. 2015, 10(5), 1340-1351. (IF: 5.44)

9.      Yan, J.*, Liu, Y., Wang, C., Han, B., and Li, S.*Assembly of lipase and P450 fatty acid decarboxylase to constitute a novel biosynthetic pathway for production of 1-alkenes from renewable triacylglycerols and oils. Biotechnol. Biofuels 2015, 8, 34. (IF: 6.044)

10.  Zhang, W., Cao, S., Qiu, L., Qi, F., Li, Z., Yang, Y., Huang, S., Bai, F., Liu, C., Wan, X.,* and Li, S.* Functional Characterization of MpaG’, the O-Methyltransferase Involved in the Biosynthesis of Mycophenolic Acid, Chembiochem 2015, 16(4), 565-569. (IF: 3.088)

11.  Huang, S.*, Gao, J., Wu, R., Li, S. and Bai Z.* Polydimethylsiloxane: A General Matrix for High-Performance Chromatographic NMR Spectroscopy. Angew. Chem. Intl. Ed. 2014, 53, 11592-11595. (IF: 11.34)

12.  Zhang, W., Xu, L., Yang, L., Huang, Y., Li, S., and Shen. Y.* Phomopsidone A, a novel depsidone metabolite from the mangrove endophytic fungus Phomopsis sp. A123. Fitoterapia 2014, 96, 146-151. (IF: 2.216)

13.  Yan, J.*, Zheng, X., Du, L., and Li, S.* Integrated lipase production and in situ biodiesel synthesis in a recombinant Pichia pastoris yeast: an efficient dual biocatalytic system composed of cell free enzymes and whole cell catalysts. Biotechnol. Biofuels 2014, 7, 55. (IF: 6.221)

14.  Wang, Q., Huang, X., Zhang, J., Lu, X., Li, S., and Li, J.-J.* Engineering self-sufficient aldehyde deformylating oxygenase fused to alternative electron transfer systems for efficient conversion of aldehydes into alkanes. Chem. Comm. 2014, 50, 4299-4301. (IF: 6.718)

15.  Zhang, W., Liu, Y., Yan, J., Cao, S., Bai, F., Yang, Y., Huang, S., Yao, L., Anzai, Y., Kato, F., Podust, L. M., Sherman, D. H.*, and Li, S.* New reactions and products resulting from alternative interactions between the P450 enzyme and redox partners. J. Am. Chem. Soc. 2014, 136(9), 3640-3646. (IF: 10.677, Faculty of 1000 Prime: http://f1000.com/prime/718276150)

16.  Liu, Y., Wang, C., Yan, J., Zhang, W., Guan, W., Lu, X., and Li, S.* Hydrogen peroxide-independent production of α-alkenes by OleT_JE P450 fatty acid decarboxylase. Biotechnol. Biofuels 2014, 7, 28. (IF: 6.221)

17.  Yan, J.*, Zheng, X., and Li, S.* A novel and robust recombinant Pichia pastoris yeast whole cell biocatalyst with intracellular overexpression of a Thermomyces lanuginosus lipase: preparation, characterization and application in biodiesel production. Bioresour. Technol. 2014, 151, 43-48. (IF: 5.039)

18.  Zhang W., Fortman, J. L., Carlson, J. C., Yan, J., Liu, Y., Bai, F., Guan, W., Jia, J., Matainaho, T., Sherman, D. H.*, and Li, S.* Characterization of the bafilomycin biosynthetic gene cluster from Streptomyces lohii. Chembiochem, 2013, 14(3), 301-306. (IF: 3.740)

19.  Sunderhaus, J. D., McAfoos, T. J., Finefield, J. M., Kato, H., Li, S., Tsukamoto, S., Sherman, D. H., and Williams, R. M.* Synthesis and bioconversions of notoamide T: a biosynthetic precursor to stephacidin A and notoamide B. Org. Lett. 2013, 15(1), 22-25. (IF: 6.142)

20.  Li, S., Tietz, D. R., Rutaganira, F. U., Kells, P. M., Anzai, Y., Kato, F., Pochapsky, T. C., Sherman, D. H., and Podust, L. M.* Substrate recognition by the multifunctional cytochrome P450 MycG in mycinamicin hydroxylation and epoxidation reactions. J. Biol. Chem. 2012, 287(45), 37880-37890. (IF: 4.773)

21.  Anzai, Y., Tsukada, S. I., Sakai, A., Masuda, R., Harada, C., Domeki, A., Li, S., Kinoshita, K., Sherman, D. H., and Kato, F.* Function of the cytochrome P450 enzymes MycCI and MycG in Micromonospora griseorubida, a producer of the macrolide antibiotic mycinamicin. Antimicrob. Agents Chemother. 2012, 56(7), 3648-3656. (IF: 4.841)

22.  Li, S., Anand, K., Tran, H., Yu, F., Finefield, J. M., Sunderhaus, J. D., McAfoos, T. J., Tsukamoto, S., Williams, R. M., and Sherman, D. H.* Comparative analysis of the biosynthetic systems for fungal bicyclo[2.2.2]diazaoctane indole alkaloids: the (+)/(-)-notoamide, paraherquamide and malbrancheamide pathways. MedChemComm 2012, 3(8), 987-996. (IF: 2.800)

23.  Li, S., Finefield, J. M., Sunderhaus, J. D., McAfoos, T. J., Williams, R. M., and Sherman, D. H.* Biochemical characterization of NotB as an FAD-dependent oxidase in the biosynthesis of notoamide indole alkaloids. J. Am. Chem. Soc. 2012, 134(2), 788-791. (IF: 9.907)

24.  Akey, D. L., Li, S., Konwerski, J., Confer, L., Bernard, S. M., Anzai, Y., Kato, F., Sherman, D. H., and Smith, J. A.* New structural form in the SAM/metal-dependent O-methyltransferase family: MycE from the mycinamycin biosynthetic pathway. J. Mol. Biol. 2011, 413(2), 438-450. (IF: 4.001)

25.  Carlson, J. C.^#, Li, S. ^#, Gunatilleke, S. S., Anzai, Y., Burr, D. A., Podust, L. M., and Sherman, D. H.* Tirandamycin biosynthesis is mediated by co-dependent oxidative enzymes. Nat. Chem. 2011, 3(8), 628-633. (*Co-first authors) (IF: 20.524)

26.  Ding, Y., de Wet, J. R., Cavalcoli, J., Li, S., Greshock, T. J., Miller, K. A., Finefield, J. M., Sunderhaus, J. D., McAfoos, T. J., Tsukamoto, S., Williams, R. M., and Sherman, D. H.* Genome-based characterization of two prenylation steps in the assembly of the stephacidin and notoamide anticancer agents in a marine-derived Aspergillus sp. J. Am. Chem. Soc. 2010, 132(36), 12733-12740. (IF: 8.580)

27.  McAfoos, T. J., Li, S., Tsukamoto, S., Sherman, D. H., and Williams, R. M.* Studies on the Biosynthesis of the stephacidins and notoamides. Total synthesis of notoamides. Heterocycles 2010, 82(1), 461-472. (IF: 1.165)

28.  Tsukada, S., Anzai, Y., Li, S., Kinoshita, K., Sherman, D. H., and Kato, F.* Gene targeting for O-methyltransferase genes, mycE and mycF, on the chromosome of Micromonospora griseorubida producing mycinamicin with a disruption cassette containing the bacteriophage φC31 attB attachment site. FEMS Microbiol. Lett. 2010, 304(2), 148-156. (IF: 2.199)

29.  Carlson, J. C., Fortman, J. L., Anzai, Y., Li, S., Douglas, A. B., and Sherman, D. H.* Identification of the tirandamycin biosynthetic gene cluster from Streptomyces sp. 307-9. Chembiochem 2010, 11(4), 564-572. (IF: 3.824)

30.  Li, S., Chaulagain, M. R., Knauff, A. R., Podust, L. M., Montgomery, J.*, and Sherman, D. H.* Selective oxidation of carbolide C-H bonds by an engineered macrolide P450 monooxygenase. Proc. Natl. Acad. Sci. U.S.A. 2009, 106(44), 18463-18468. (IF: 9.380)

31.  Carlson, J. C. ^#, Li, S.^#, Douglas, A. B., and Sherman, D. H.* Isolation and characterization of tirandamycins from a marine-derived Streptomyces sp. J. Nat. Prod. 2009, 72(11), 2076-2079. (*Co-first authors) (IF: 2.843)

32.  Li, S., Anzai, Y., Kinoshita, K., Kato, F., Sherman, D. H.* Functional analysis of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin macrolide antibiotics. Chembiochem 2009, 10(8), 1297-1301. (IF: 3.322)

33.  Li, S., Ouellet, H., Sherman, D. H., Podust, L. M.* Analysis of transient and catalytic desosamine-binding pockets in cytochrome P-450 PikC from Streptomyces venezuelae. J. Biol. Chem. 2009, 284(9), 5723-5730. (IF: 5.520)

34.  Anzai, Y. ^#, Li, S. ^#, Chaulagain, M. R., Kinoshita, K., Kato, F., Montgomery, J., Sherman, D. H.* Functional analysis of MycCI and MycG, cytochrome P450 enzymes involved in biosynthesis of mycinamicin macrolide antibiotics. Chem. Biol. 2008, 15(9), 950-959. (*Co-first authors) (IF: 5.603)

35.  Li, S., Podust, L. M., Sherman, D. H.* Engineering and analysis of a self-sufficient biosynthetic cytochrome P450 PikC fused to the RhFRED reductase domain. J. Am. Chem. Soc. 2007, 129(43), 12940-12941. (IF: 7.885)

36.  Li, S., Grüschow, S., Dordick, J. S., Sherman, D. H.* Molecular analysis of the role of tyrosine 224 in the active site of Streptomyces coelicolor RppA, a bacterial type III polyketide synthase. J. Biol. Chem. 2007, 282(17), 12765-12772. (IF: 5.851)

37.  Sherman, D. H., Li, S., Yermalitskaya, L. V., Kim, Y., Smith, J. A., Waterman, M. R., Podust, L. M. The structural basis for substrate anchoring, active site selectivity, and product formation by P450 PikC from Streptomyces venezuelae. J. Biol. Chem. 2006, 281(36), 26289-26297. (IF: 5.808)

38.  Li, S., Wang, J., Zheng, Z.,  Xu, Q., Huang, Y., Zhao, Y., Su, W.* 1-(2,4-Dihydroxy-3,5-Dimethylphenyl)-ethanone (Clavatol). Acta. Cryst. 2003, E59, o1649-o1650. (IF: 0.508)

39.  Li, S., Zheng, Z. Discovery and development of marine antitumor natural products. Marine Science Bulletin 2003, 22(2), 76-82.

40.  Zhang, J., Zheng, Z., Huang, Y., Li, S., Su, W.* Preliminary research on antitumor activities of natural products from algal fungi. J. Xiamen Univ. (Natural Science) 2004, 43 (4), 551-556.

1.      Chimeric Cytochrome P450 Proteins and Methods of Use. LI, Shengying, and SHERMAN, David H. (US 20090081758A3)

2.      A Method for Selective Oxidation of C-H Bonds by an Engineered P450 Enzyme. LI, Shengying, SHERMAN, David H., CHAULAGAIN, Mani Raj, MONTGOMERY, John, and KNAUFF, Allison R. (WO 2011038313A2)

3.      Biosynthetic Systems Producing Fungal Indole Alkaloids LI, Shengying, David H. Sherman, Krithika Anand, and Robert M. Williams (WO 2013152110A1)

4.      阎金勇, 李盛英. 一种利用偶联生产脂肪酶的方式催化生产生物柴油的方法（申请号201410138507.3，申请公布号：CN103923954A）

5.      李盛英，刘奕，吕雪峰，刘旭峰. 一种生产脂肪端烯的方法及其应用（申请号201410660346.4）

6.      阎金勇, 李盛英. 一种基于脂肪酶与P450脂肪酸脱羧酶偶联催化的脂肪烯烃催化合成方法（申请号201510003451.5，申请公布号：CN104561152A）

7.      李盛英，马莉，Eung-Soo Kim. 一种制备毛发生长促进剂的生物转化方法（申请号201510346350.8）

8.      李盛英，杜磊，刘双江. 一种酚类化合物催化氧化系统及其应用（申请号:201610015698.3）

9.      高效分泌表达黑曲霉α-半乳糖苷酶AGA的工程菌及其构建和应用（申请号：2015107066150，申请公布号：CN105219791A）

1. “Redox Partner Proteins: Underexplored Function Modulators for Cytochrome P450 Enzymes from Actinomycetes”, oral, 2015 International Symposium & Annual Meeting of The Korean Society for Microbiology and Biotechnology, Gyeongju, Korea, June 25, 2015.

2. “Multifunctional biosynthetic P450 enzymes from actinomycetes”, oral, The 2nd Chinese Conference of Actinomycetes Biology and Application, Guangzhou, China, December 9, 2014.

3. “Alternative interactions between cytochrome P450 enzymes and redox partner proteins”, oral, The 2014 Annual Meeting of Chinese Society for Microbiology, Harbin, China, August 17, 2014.

4. “Uncovering the broader roles of redox partner proteins for cytochrome P450 enzymes”, oral, The 16th European Congress of Biotechnology, Edinburgh, UK, July 16, 2014.

5. “A new microbial cell factory for production of 1-alkenes”, oral, The 1st German-Chinese Workshop on “Biotechnology in a Bioeconomy, Karlsruhe, Germany, May 28, 2014.

6. “Harnessing The Native Substrate Flexibility of Biosynthetic P450 Enzymes for Natural Product Structural Diversification”, oral, The American Chemical Society 243rd National Meeting, San Diego, CA, March 26, 2012.

7. “Selective Oxidation of Carbolides C-H Bonds by An Engineered Macrolide P450 Monooxygenase”, poster, Midwest Enzyme Chemistry Conference 2008, University of Chicago, Chicago, IL, October 4, 2008; Midwest Enzyme Chemistry Conference 2009, Loyola University, Chicago, IL, October 10, 2009.

8. “Structural, Biochemical, and Bioengineering Study of PikC, a Cytochrome P450 Enzyme from Macrolide Antibiotic Pikromycin Biosynthetic Pathway in Streptomyces venezuelae”, oral, Heme Team Meeting, University of Michigan, Ann Arbor, MI, December 13, 2007.

9. “Bioengineering of PikC, A Bacterial Biosynthetic Cytochrome P450”, oral, LSI Colloquium 2007, University of Michigan, Ann Arbor, MI, November 16, 2007

1. 10/2015-03/2017 Improving C12 activity of new decarboxylase encoding sequences, TOTAL, Research Program Agreement between TOTAL Marketing Services and QIBEBT.

2. 10/2013-09/2014 Production of α-alkenes with aviation fuel properties in Escherichia coli, BOEING, QIBEBT Collaboration Agreement for a Joint Research Laboratory for Sustainable Aviation Biofuels.

Yi Liu  01  19183  

Bo Fang  02  63228 

Xianliang Zheng  01  19183  

Zhong Li  02  19183  

Linlin Ning  02  63228  

Lei Du  01  19178  

Zepeng Qu  02  63228  

Yuanyuan Jiang  01  19178  

1. Aug. 2014      The National Science Fund for Excellent Young Scholars.

2. Feb. 2012      The Recruitment Program of Global Young Experts.

3. Sept. 2011      Michigan Memorial Phoenix Energy Institute (MMPEI) Postdoctoral Fellowship.

4.  Apr. 2010      National Award for Outstanding Chinese PhD Student Abroad 2009.

5.  Dec. 2005      NR-BLAKE,MR. AND MRS. EDWARD Fellowship, College of Pharmacy, The University of Michigan.