基于MALDI-TOF MS以及LC-MS/MS等软电离质谱，发展分子指纹图谱采集、化学标记时空蛋白组学、多组学联合分析等质谱分析新技术，以此寻找、鉴定疾病标志物分子并获取关键蛋白翻译后修饰等信息, 结合人工智能方法分析挖掘关键质谱信息，开发可用于细菌感染以及恶性肿瘤早期筛查或诊断的快速高通量方法，探究耐药性形成机制及阻断方法。

1. 质谱分析技术
2. 蛋白质组学技术
3. 微生物感染诊断与耐药性机制研究
4. 癌症诊断及耐药性机制研究

招生专业：

药学

生物技术与工程

基于组学分析的药物靶点发现
质谱与蛋白组学新技术研究
癌症与细菌感染的诊断技术研发与耐药性机制研究

2014-10--2018-09   瑞士洛桑联邦理工学院   博士
2011-09--2014-06   南京大学   硕士
2007-09--2011-06   南京大学   本科

   

2022-11~现在, 中国科学院杭州医学研究所, 研究员
2019-01~2019-08,美国斯坦福大学, 访问学者
2018-10~2022-01,瑞士洛桑联邦理工学院, 博士后研究员

2024-01-01-2026-12-31,浙江省抗癌协会专业委员会, 委员

   

（ 1 ） Maldi device and methods of preparation and use thereof, 发明专利, 2019, 专利号: GB201700908(D0)

（ 2 ） Detection of Bacteria, 发明专利, 2019, 第 1 作者, 专利号: EP378170A

（ 3 ） Skin cell analysis, 发明专利, 2020, 第 1 作者, 专利号: GB2576374(A)

   

[1] Wang, Ya, Zhang, Guixi, Zhou, Zhilan, Zhang, Ning, Jiang, Hang, Liu, Yichang, Fu, Ting, Zhu, Yingdi, Li, Juan. Antitumor Activity of a Bispecific Chimera Targeting EGFR and Met in Gefitinib-Resistant Non-Small Cell Lung Cancer. ADVANCED HEALTHCARE MATERIALS[J]. 2025, 第 8 作者14(2): http://dx.doi.org/10.1002/adhm.202402884.
[2] Hou, Peidong, Sipei Wang, Zhentao Shao, Yingdi Zhu, Juan Li. Off-Target Interactions of Vancomycin with Vascular Wall Involving Elastin-Induced Self-Assembly. Analytical Chemistry[J]. 2025, 第 4 作者97(null): 7107-7117, https://pubs.acs.org/doi/epdf/10.1021/acs.analchem.4c06259?ref=article_openPDF.
[3] Su, Min, Peng, Tao, Zhu, Yingdi, Li, Juan. Nucleic Acid Covalent Tags. CHEMBIOCHEM[J]. 2025, 第 3 作者  通讯作者  26(5): http://dx.doi.org/10.1002/cbic.202400805.
[4] Zhilan Zhou, Yichang Liu, Ya Wang, Hang Jiang, Tingting Chen, Yingdi Zhu, Ting Fu, Juan Li. Engineering aptamer-directed phosphatase recruiting chimeras: a strategy for modulating receptor function and overcoming drug resistance. NATURE COMMUNICATIONS[J]. 2025, 第 6 作者16(1): 1-18, https://doaj.org/article/0476b49f602a4cf7ae627f8f8b89ce33.
[5] Wang, Ya, Yingdi Zhu, Juan Li. Bispecific Nanobody-Aptamer Conjugates for Enhanced Cancer Therapy in Solid Tumors. Small[J]. 2024, 第 2 作者
[6] JIANG Yan, ZHU Yingdi. Bacterial Protein Profiling: Comparison of Three Mass Spectrometry Methodologies. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES[J]. 2024, 第 2 作者  通讯作者  on-line(null): 
[7] Tzu-En Lin, Yingdi Zhu, YuehTien Hsu, FangYu Liu, YenPin Lin, ChaoMin Cheng. In situ detection of multitarget impurities on contact lens by electrochemical scanning probe. SENSORS AND ACTUATORS: B. CHEMICAL[J]. 2023, 第 2 作者374: http://dx.doi.org/10.1016/j.snb.2022.132855.
[8] Zhentao Shao, Yingdi Zhu, Juan Li. Bioorthogonal Probes for Analyzing Non-Enzymatic Post-Translational Modifications. ChemBioChem[J]. 2023, 第 2 作者24(9): https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/cbic.202200763.
[9] Zhou, Zhilan, Wang, Ya, Shao, Zhengtao, Zhang, Guixi, Jiang, Hang, Tang, Yiyuan, Huang, Zening, Zhu, Yingdi, Li, Juan. A multiparametric fluorescent visualization approach for detecting drug resistance in living cancer cells. TALANTA[J]. 2023, 第 8 作者259: http://dx.doi.org/10.1016/j.talanta.2023.124564.
[10] Yingdi Zhu, Hubert H Girault. Algorithms push forward the application of MALDI–TOF mass fingerprinting in rapid precise diagnosis. VIEW[J]. 2023, 第 1 作者  通讯作者  4(2): 1-10, https://doaj.org/article/f7be498500ce4489888c2a144ffd6d19.
[11] Jia Liu, Qiao Duan, Zhentao Shao, Kaiming Chen, Yingdi Zhu, Juan Li, Weihong Tan. Formaldehyde Cross-Linking-Assisted Phase Separation for Protein Aptamer Selection. Analytical Chemistry[J]. 2023, 第 5 作者95(16): 6700-6708, https://pubs.acs.org/doi/abs/10.1021/acs.analchem.3c00434.
[12] Yingdi Zhu, Andreas Lesch, Xiaoyun Li, TzuEn Lin, Natalia Gasilova, Milica Jovic, Horst Matthias Pick, PingChih Ho, Hubert H Girault. Rapid Noninvasive Skin Monitoring by Surface Mass Recording and Data Learning. JACS AU[J]. 2021, 第 1 作者1(5): 598-611, https://doaj.org/article/96d4b6bb8f2e497191395f34455f686a.
[13] Nagar, Bhawna, Jovic, Milica, Bassetto, Victor Costa, Zhu, Yingdi, Pick, Horst, GomezRomero, Pedro, Merkoci, Arben, Girault, Hubert H, Lesch, Andreas. Highly Loaded Mildly Edge-Oxidized Graphene Nanosheet Dispersions for Large-Scale Inkjet Printing of Electrochemical Sensors. CHEMELECTROCHEM[J]. 2020, 第 4 作者7(2): 460-468, https://www.webofscience.com/wos/woscc/full-record/WOS:000514380800012.
[14] KI Hilgendorf, CT Johnson, K Han, A Rabiee, R Cheng, Yingdi Zhu, Katrin J Svensson, Michael C Bassik, Peter K Jackson. A CRISPR-based genome-wide screen for adipogenesis reveals new insights into mitotic expansion and lipogenesis. bioRxiv. 2020, 第 6 作者https://www.biorxiv.org/content/10.1101/2020.07.13.201038v1.abstract.
[15] Yingdi ZHU, Milica Jovic, Andreas Lesch, Horst Pick, Lysiane Tissières Lovey, Dr. Michel Prudent, Hubert Girault. Immunaffine Amperometrische Detektion Bakterieller Infektionen. Angewandte Chemie[J]. 2019, 第 1 作者130: 15158-15162, https://onlinelibrary.wiley.com/doi/10.1002/ange.201808666.
[16] Zhu, Yingdi, Pick, Horst, Gasilova, Natalia, Li, Xiaoyun, Lin, TzuEn, Laeubli, Heinz Philipp, Zippelius, Alfred, Ho, PingChih, Girault, Hubert H. MALDI Detection of Exosomes: A Potential Tool for Cancer Studies. CHEM[J]. 2019, 第 1 作者5(5): 1318-1336, http://dx.doi.org/10.1016/j.chempr.2019.04.007.
[17] Darvishi, Sorour, Pick, Horst, Lin, TzuEn, Zhu, Yingdi, Li, Xiaoyun, Ho, PingChih, Girault, Hubert H, Lesch, Andreas. Tape-Stripping Electrochemical Detection of Melanoma. ANALYTICAL CHEMISTRY[J]. 2019, 第 4 作者91(20): 12900-12908, https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::e3f0365e5f97bfaea9973ae7331c8eb0.
[18] Zhu, Yingdi, Jovic, Milica, Lesch, Andreas, Lovey, Lysiane Tissieres, Prudent, Michel, Pick, Horst, Girault, Hubert H. Immuno-affinity Amperometric Detection of Bacterial Infections. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION[J]. 2019, 第 1 作者57(45): 14942-14946, http://dx.doi.org/10.1002/anie.201808666.
[19] Zhu, Yingdi, Gasilova, Natalia, Jovic, Milica, Qiao, Liang, Liu, Baohong, Lovey, Lysiane Tissieres, Pick, Horst, Girault, Hubert H. Detection of antimicrobial resistance-associated proteins by titanium dioxide-facilitated intact bacteria mass spectrometry. CHEMICAL SCIENCE[J]. 2018, 第 1 作者9(8): 2212-2221, https://www.webofscience.com/wos/woscc/full-record/WOS:000427091500018.
[20] Jovic, Milica, Zhu, Yingdi, Lesch, Andreas, Bondarenko, Alexandra, CortesSalazar, Fernando, Gumy, Frederic, Girault, Hubert H. Inkjet-printed microtiter plates for portable electrochemical immunoassays. JOURNAL OF ELECTROANALYTICAL CHEMISTRY[J]. 2017, 第 2 作者786: 69-76, http://dx.doi.org/10.1016/j.jelechem.2016.12.051.
[21] Zhu, Yingdi, Qiao, Liang, Prudent, Michel, Bondarenko, Alexandra, Gasilova, Natalia, Moller, Siham Beggah, Lion, Niels, Pick, Horst, Gong, Tianqi, Chen, Zhuoxin, Yang, Pengyuan, Loveyf, Lysiane Tissieres, Girault, Hubert H. Sensitive and fast identification of bacteria in blood samples by immunoaffinity mass spectrometry for quick BSI diagnosis. CHEMICAL SCIENCE[J]. 2016, 第 1 作者7(5): 2987-2995, https://www.webofscience.com/wos/woscc/full-record/WOS:000374859300003.
[22] Bondarenko, A, Zhu, Y, Qiao, L, Salazar, F Cortes, Pick, H, Girault, H H. Aluminium foil as a single-use substrate for MALDI-MS fingerprinting of different melanoma cell lines. ANALYST[J]. 2016, 141(11): 3403-3410, https://www.webofscience.com/wos/woscc/full-record/WOS:000377225600031.
[23] Jun-Tao Cao, Ying-Di Zhu, Rohit Kumar Rana, Jun-Jie Zhu. Microfluidic chip integrated with flexible PDMS-based electrochemical cytosensor for dynamic analysis of drug-induced apoptosis on HeLa cells. BIOSENSORS AND BIOELECTRONICS. 2014, 第 2 作者51: 97-102, http://dx.doi.org/10.1016/j.bios.2013.07.025.
[24] Peng, Juan, Zhu, YingDi, Li, XingHua, Jiang, LiPing, AbdelHalim, E S, Zhu, JunJie. Electrochemical immunoassay for the prostate specific antigen using ceria mesoporous nanospheres. MICROCHIMICA ACTA[J]. 2014, 第 2 作者181(13-14): 1505-1512, http://dx.doi.org/10.1007/s00604-014-1193-x.
[25] Zhu, YingDi, Peng, Juan, Jiang, LiPing, Zhu, JunJie. Fluorescent immunosensor based on CuS nanoparticles for sensitive detection of cancer biomarker. ANALYST[J]. 2014, 第 1 作者139(3): 649-655, https://www.webofscience.com/wos/woscc/full-record/WOS:000328912000017.
[26] Feng, LiNa, Bian, ZhiPing, Peng, Juan, Jiang, Fang, Yang, GuoHai, Zhu, YingDi, Yang, Di, Jiang, LiPing, Zhu, JunJie. Ultrasensitive Multianalyte Electrochemical Immunoassay Based on Metal Ion Functionalized Titanium Phosphate Nanospheres. ANALYTICAL CHEMISTRY[J]. 2012, 第 6 作者84(18): 7810-7815, https://www.webofscience.com/wos/woscc/full-record/WOS:000308829700034.
[27] Cao, JunTao, Hao, XiaoYao, Zhu, YingDi, Sun, Ken, Zhu, JunJie. Microfluidic Platform for the Evaluation of Multi-Glycan Expressions on Living Cells using Electrochemical Impedance Spectroscopy and Optical Microscope. ANALYTICAL CHEMISTRY[J]. 2012, 第 3 作者84(15): 6775-6782, https://www.webofscience.com/wos/woscc/full-record/WOS:000307159200072.
[28] Feng, LiNa, Peng, Juan, Zhu, YingDi, Jiang, LiPing, Zhu, JunJie. Synthesis of Cd2+-functionalized titanium phosphate nanoparticles and application as labels for electrochemical immunoassays. CHEMICAL COMMUNICATIONS[J]. 2012, 第 3 作者48(37): 4474-4476, https://www.webofscience.com/wos/woscc/full-record/WOS:000302559100025.

（1） MALDI-TOF Mass Fingerprinting for Rapid Identification of Bacteria: Experimental Procedures, Royal Society of Chemistry, 2023-10, 第 1 作者

   

（ 1 ） 循环细菌外泌体多组分质谱用于胰腺癌演进研究, 负责人, 国家任务, 2024-01--2026-12
（ 2 ） 2023年度钱塘交叉基金项目, 负责人, 地方任务, 2024-01--2024-12
（ 3 ） 具有MDM2和PARP-1双重抑制作用的新型抗肿瘤化合 物的设计、合成与生物活性研究, 参与, 国家任务, 2023-01--2026-12
（ 4 ） 中科院医学所肿瘤智能分子诊断创新团队, 参与, 地方任务, 2023-01--2025-12
（ 5 ） 中国科学院杭州医学研究所人才启动项目, 负责人, 研究所自主部署, 2022-11--2027-11

（1）基于质谱的皮肤癌检测新方法   2023 年中国化学会年会   2023-06-17
（2）Immuno-Affinity Electrochemical Detection of Bacterial Infections   2019-09-02
（3）MALDI Detection of Exosomes for Cancer Studies   2019-06-01
（4）Detection of Marker Proteins for Bacteria Antimicrobial Resistance by Mass Spectrometry   2018-05-23
（5）Spectra Pattern Matching with In Silico Hypothesis Validation for Bacterial Typing   2017-07-09
（6）Mass Spectrometry Methods for Bacterial Infection Diagnosis   2017-04-03