真核生物的多种生命活动，如DNA复制、基因转录、DNA损伤修复、基因组稳定性维持等，均受到表观遗传机制的调控。

染色质的动态变化调节DNA的折叠方式，是表观遗传调控的重要基础。DNA和组蛋白的分子结构、组成形式的改变能够造成

染色质结构的动态变化，并与众多表观因子（如组蛋白变体、组蛋白伴侣、组蛋白修饰酶、染色质重塑复合物、非编码RNA

等）一起调控染色质动态，确保生命活动的有序进行。本课题组致力于研究染色质动态的调控及其表观遗传功能。其主要

发现包括组蛋白变体的识别机制、"核小体编辑"的基本规律、DNA损伤修复途径的决定机制等。

　 "核小体编辑"是染色质重构复合物催化组蛋白变体H2A.Z替换常规H2A，并将H2A.Z精准定位到基因组特定区域的过程，其

功能异常与癌症等多种疾病密切相关。我们希望阐明核小体编辑的作用机制，实现核小体编辑的人工操控。细胞对DNA损伤

修复途径的决定机制对于基因组稳定性维持至关重要，DNA损伤"合成致死"效应的发现，催生了PAPR抑制剂等一线抗癌药物。

我们希望研究细胞选择不同修复途径进行DNA损伤修复的作用机制，寻找新的药物靶点用于抗癌药物的研发。

  我们将运用生物化学、生物物理学、结构生物学、酵母遗传学、单分子力谱等方法开展上述研究。

  主页：https://ibp.cas.cn/kydw_157813/cxyjqtxmhdz/201912/t20191202_5447222.html

  Email:zhouzh@ibp.ac.cn

  Tel:010-64889862

欢迎对表观遗传和蛋白质分子设计感兴趣的同学加入我们的集体！

071010-生物化学与分子生物学
071007-遗传学
071011-生物物理学

染色质结构及表观调控
结构生物学
蛋白质工程

1999-09--2003-08   中科院上海生物化学与细胞生物学研究所   理学博士
1992-09--1999-07   湖南师范大学   理学学士硕士

   

2008-09~2011-09,美国国立健康研究院癌症研究所, Research Fellow
2003-09~2008-09,美国国立健康研究院癌症研究所, Visiting Fellow

2018-08-01-2021-10-31,中国生物物理学会11届理事会, 会员工作委员会主任
2017-11-30-今,《生命科学研究》学术期刊, 编委

结构生物学技术
细胞的物理生物学
高等生物物理学

   

   

[1] Li, Wenyan, Hu, Jie, Song, Feng, Yu, Juan, Peng, Xin, Zhang, Shuming, Wang, Lin, Hu, Mingli, Liu, JiaCheng, Wei, Yu, Xiao, Xue, Li, Yan, Li, Dongyu, Wang, Hui, Zhou, BingRui, Dai, Linchang, Mou, Zongjun, Zhou, Min, Zhang, Haonan, Zhou, Zheng, Zhang, Huidong, Bai, Yawen, Zhou, JinQiu, Li, Wei, Li, Guohong, Zhu, Ping. Structural basis for linker histone H5-nucleosome binding and chromatin fiber compaction. CELL RESEARCH. 2024, 第 20 作者http://dx.doi.org/10.1038/s41422-024-01009-z.
[2] Li Huang, Zheng Zhou. Protocol for preparing SUV420H1 in complex with the nucleosome containing H2A.Z and H4K20Ecx for structure determination. STAR PROTOCOLS[J]. 2024, 第 2 作者5(3): 103295, https://doaj.org/article/c0419efcd6d6408eb62d9920c08ec4ab.
[3] Xiao, Shumin, Wang, Yajing, Shan, Shan, Zhou, Zheng. The interplay between viral molecular mimicry and host chromatin dynamics. NUCLEUS[J]. 2023, 第 4 作者  通讯作者  14(1): http://dx.doi.org/10.1080/19491034.2023.2216560.
[4] Li Huang, Youwang Wang, Haizhen Long, Haoqiang Zhu, Zengqi Wen, Liwei Zhang, Wenhao Zhang, Zhenqian Guo, Longge Wang, Fangyi Tang, Jie Hu, Keyan Bao, Ping Zhu, Guohong Li, Zheng Zhou. Structural insight into H4K20 methylation on H2A.Z-nucleosome by SUV420H1. MOLECULAR CELL[J]. 2023, 第 15 作者  通讯作者  83(16): 2884-2895, http://dx.doi.org/10.1016/j.molcel.2023.07.001.
[5] Chen, Jiao, Lu, Zuer, Gong, Weibin, Xiao, Xue, Feng, Xiaoli, Li, Wei, Shan, Shan, Xu, Dongyi, Zhou, Zheng. Epstein-Barr virus protein BKRF4 restricts nucleosome assembly to suppress host antiviral responses. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2022, 第 9 作者  通讯作者  119(37): http://dx.doi.org/10.1073/pnas.2203782119.
[6] 周政. 周政：因材施教 因地制宜 寓研于乐. 中国科技教育[J]. 2022, 第 1 作者34-35, http://lib.cqvip.com/Qikan/Article/Detail?id=7106845778.
[7] Shi, Liuxin, Huang, Li, Long, Haizhen, Song, Aoqun, Zhou, Zheng. Structural basis of nucleosomal H4K20 methylation by methyltransferase SET8. FASEB JOURNAL[J]. 2022, 第 5 作者  通讯作者  36(6): https://www.doi.org/10.1096/fj.202101821R.
[8] Dai, Linchang, Xiao, Xue, Pan, Lu, Shi, Liuxin, Xu, Ning, Zhang, Zhuqiang, Feng, Xiaoli, Ma, Lu, Dou, Shuoxing, Wang, Pengye, Zhu, Bing, Li, Wei, Zhou, Zheng. Recognition of the inherently unstable H2A nucleosome by Swc2 is a major determinant for unidirectional H2A.Z exchange. CELL REPORTS[J]. 2021, 第 13 作者  通讯作者  35(8): http://dx.doi.org/10.1016/j.celrep.2021.109183.
[9] Zhou, Ning, Shi, Liuxin, Shan, Shan, Zhou, Zheng. Molecular basis for the selective recognition and ubiquitination of centromeric histone H3 by yeast E3 ligase Psh1. JOURNAL OF GENETICS AND GENOMICS[J]. 2021, 第 4 作者  通讯作者  48(6): 463-472, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=7064902&detailType=1.
[10] Zhou, Min, Dai, Linchang, Li, Chengmin, Shi, Liuxin, Huang, Yan, Guo, Zhenqian, Wu, Fei, Zhu, Ping, Zhou, Zheng. Structural basis of nucleosome dynamics modulation by histone variants H2A.B and H2AZ2.2. EMBO JOURNAL[J]. 2021, 第 9 作者  通讯作者  40(1): http://dx.doi.org/10.15252/embj.2020105907.
[11] Dai, Linchang, Dai, Yaxin, Han, Jinhua, Huang, Yan, Wang, Longge, Huang, Jun, Zhou, Zheng. Structural insight into BRCA1-BARD1 complex recruitment to damaged chromatin. MOLECULAR CELL[J]. 2021, 第 7 作者  通讯作者  81(13): 2765-+, http://dx.doi.org/10.1016/j.molcel.2021.05.010.
[12] Min Zhou, Linchang Dai, Chengmin Li, Liuxin Shi, Yan Huang, Zhenqian Guo, Fei Wu, Ping Zhu, Zheng Zhou. Structural basis of nucleosome dynamics modulation by histone variants H2A.B andH2A.Z.2.2. THE EMBO JOURNAL. 2020, 第 9 作者40(1): 
[13] Huang, Yan, Sun, Lu, Pierrakeas, Leonidas, Dai, Linchang, Pan, Lu, Luk, Ed, Zhou, Zheng. Role of a DEF/Y motif in histone H2A-H2B recognition and nucleosome editing. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2020, 第 7 作者  通讯作者  117(7): 3543-3550, http://dx.doi.org/10.1073/pnas.1914313117.
[14] Dai, Yaxin, Zhang, Fan, Wang, Longge, Shan, Shan, Gong, Zihua, Zhou, Zheng. Structural basis for shieldin complex subunit 3?mediated recruitment of the checkpoint protein REV7 during DNA double-strand break repair. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2020, 第 6 作者  通讯作者  295(1): 250-262, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952594/.
[15] Huang, Yan, Dai, Yaxin, Zhou, Zheng. Mechanistic and structural insights into histone H2A-H2B chaperone in chromatin regulation. BIOCHEMICAL JOURNAL. 2020, 第 3 作者  通讯作者  477(17): 3367-3386, http://dx.doi.org/10.1042/BCJ20190852.
[16] Gao, Ying, Ren, RuJing, Zhong, ZiLin, Dammer, Eric, Zhao, QianHua, Shan, Shan, Zhou, Zheng, Li, Xia, Zhang, YueQi, Cui, HaiLun, Hu, YongBo, Chen, ShengDi, Chen, JianJun, Guo, QiHao, Wang, Gang. Mutation profile of APP, PSEN1, and PSEN2 in Chinese familial Alzheimer's disease. NEUROBIOLOGY OF AGING[J]. 2019, 第 7 作者77: 154-157, http://dx.doi.org/10.1016/j.neurobiolaging.2019.01.018.
[17] Wang, Yunyun, Liu, Sheng, Sun, Lu, Xu, Ning, Shan, Shan, Wu, Fei, Liang, Xiaoping, Huang, Yingzi, Luk, Ed, Wu, Carl, Zhou, Zheng. Structural insights into histone chaperone Chz1-mediated H2A.Z recognition and histone replacement. PLOS BIOLOGY[J]. 2019, 第 11 作者  通讯作者  17(5): http://dx.doi.org/10.1371/journal.pbio.3000277.
[18] Yaxin Dai, Fan Zhang, Longge Wang, Shan Shan, Zihua Gong, Zheng Zhou. Structural basis for shieldin complex subunit 3–mediated recruitment of the checkpoint protein REV7 during DNA double-strand break repair. THE JOURNAL OF BIOLOGICAL CHEMISTRY. 2019, 第 6 作者295(1): 250-262, http://dx.doi.org/10.1074/jbc.RA119.011464.
[19] Dai, Linchang, Xu, Ning, Zhou, Zheng. NMR investigations on H2A-H2B heterodimer dynamics conferred by histone variant H2A.Z. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2019, 第 3 作者  通讯作者  518(4): 752-758, http://dx.doi.org/10.1016/j.bbrc.2019.08.127.
[20] Sun, Wan, Shen, Yanjie, Shan, Shan, Han, Liyun, Li, Yang, Zhou, Zheng, Zhong, Zilin, Chen, Jianjun. Identification of TYR mutations in patients with oculocutaneous albinism. MOLECULAR MEDICINE REPORTS[J]. 2018, 第 6 作者17(6): 8409-8413, https://www.webofscience.com/wos/woscc/full-record/WOS:000435394000118.
[21] Huang Yan, Zhou Zheng. Recent Progress in Histone Chaperones Associated With H2A-H2B Type Histones. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2018, 第 2 作者  通讯作者  45(9): 971-980, https://www.webofscience.com/wos/woscc/full-record/WOS:000445847600010.
[22] Dai, Linchang, Xie, Xiaoyan, Zhou, Zheng. Crystal structure of the histone heterodimer containing histone variant H2A.Bbd. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2018, 第 3 作者  通讯作者  503(3): 1786-1791, http://dx.doi.org/10.1016/j.bbrc.2018.07.114.
[23] Yaxin Dai, Aili Zhang, Shan Shan, Zihua Gong, Zheng Zhou. Structural basis for recognition of 53BP1 tandem Tudor domain by TIRR. NATURE COMMUNICATIONS[J]. 2018, 第 5 作者  通讯作者  9(1): https://doaj.org/article/a2d88984045941a58372fc19fffd5812.
[24] Huang Yan, Zhou Zheng. Recent Progress in Histone Chaperones Associated With H2A-H2B Type Histones. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2018, 第 2 作者  通讯作者  45(9): 971-980, https://www.webofscience.com/wos/woscc/full-record/WOS:000445847600010.
[25] Liang, Xiaoping, Shan, Shan, Pan, Lu, Zhao, Jicheng, Ranjan, Anand, Wang, Feng, Zhang, Zhuqiang, Huang, Yingzi, Feng, Hanqiao, Wei, Debbie, Huang, Li, Liu, Xuehui, Zhong, Qiang, Lou, Jizhong, Li, Guohong, Wu, Carl, Zhou, Zheng. Structural basis of H2A.Z recognition by SRCAP chromatin-remodeling subunit YL1. NATURE STRUCTURAL & MOLECULAR BIOLOGY[J]. 2016, 第 17 作者  通讯作者  23(4): 317-323, http://dx.doi.org/10.1038/nsmb.3190.
[26] Zhong, Zilin, Yan, Min, Sun, Wan, Wu, Zehua, Han, Liyun, Zhou, Zheng, Zheng, Fang, Chen, Jianjun. Two novel mutations in PRPF3 causing autosomal dominant retinitis pigmentosa. SCIENTIFIC REPORTS[J]. 2016, 第 6 作者6: http://dx.doi.org/10.1038/srep37840.
[27] Zhou JuJun, Feng XiaoLi, Zhou Zheng. Chromatin Assembly of Histone Variants. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2015, 第 3 作者  通讯作者  42(11): 1003-1008, https://www.webofscience.com/wos/woscc/full-record/WOS:000365558600004.
[28] Mao, Zhuo, Pan, Lu, Wang, Weixiang, Sun, Jian, Shan, Shan, Dong, Qiang, Liang, Xiaoping, Dai, Linchang, Ding, Xiaojun, Chen, She, Zhang, Zhuqiang, Zhu, Bing, Zhou, Zheng. Anp32e, a higher eukaryotic histone chaperone directs preferential recognition for H2A.Z. CELL RESEARCH[J]. 2014, 第 13 作者  通讯作者  24(4): 389-399, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=5110666&detailType=1.
[29] Hong, Jingjun, Feng, Hanqiao, Zhou, Zheng, Ghirlando, Rodolfo, Bai, Yawen. Identification of Functionally Conserved Regions in the Structure of the Chaperone/CenH3/H4 Complex. JOURNAL OF MOLECULAR BIOLOGY[J]. 2013, 第 3 作者425(3): 536-545, http://dx.doi.org/10.1016/j.jmb.2012.11.021.
[30] Zhou, Zheng, Feng, Hanqiao, Zhou, BingRui, Ghirlando, Rodolfo, Hu, Kaifeng, Zwolak, Adam, Jenkins, Lisa M Miller, Xiao, Hua, Tjandra, Nico, Wu, Carl, Bai, Yawen. Structural basis for recognition of centromere histone variant CenH3 by the chaperone Scm3. NATURE[J]. 2011, 第 1 作者472(7342): 234-237, https://www.webofscience.com/wos/woscc/full-record/WOS:000289469100046.
[31] Hansen, D Flemming, Zhou, Zheng, Fen, Haniqiao, Jenkins, Lisa M Miller, Bai, Yawen, Kay, Lewis E. Binding Kinetics of Histone Chaperone Chz1 and Variant Histone H2A.Z-H2B by Relaxation Dispersion NMR Spectroscopy. JOURNAL OF MOLECULAR BIOLOGY[J]. 2009, 第 2 作者387(1): 1-9, http://dx.doi.org/10.1016/j.jmb.2009.01.009.
[32] Zhou, Zheng, Feng, Hanqiao, Ghirlando, Rodolfo, Bai, Yawen. The High-Resolution NMR Structure of the Early Folding Intermediate of the Thermus thermophilus Ribonuclease H. JOURNAL OF MOLECULAR BIOLOGY[J]. 2008, 第 1 作者384(2): 531-539, http://dx.doi.org/10.1016/j.jmb.2008.09.044.
[33] Zhou, Zheng, Feng, Hanqiao, Hansen, D Flemming, Kato, Hidenori, Luk, Ed, Freedberg, Daron I, Kay, Lewis E, Wu, Carl, Bai, Yawen. NMR structure of chaperone Chz1 complexed with histones H2A.Z-H2B. NATURE STRUCTURAL & MOLECULAR BIOLOGY[J]. 2008, 第 1 作者15(8): 868-869, https://www.webofscience.com/wos/woscc/full-record/WOS:000258191100023.
[34] Zheng Zhou, Hanqiao Feng, Yawen Bai. Detection of a hidden folding intermediate in the focal adhesion target domain: Implications for its function and folding. PROTEINS. 2006, 第 1 作者
[35] Feng, HQ, Zhou, Z, Bai, YW. A protein folding pathway with multiple folding intermediates at atomic resolution. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2005, 102(14): 5026-5031, https://www.webofscience.com/wos/woscc/full-record/WOS:000228195800021.
[36] Zheng Zhou. Structure determination and folding pathway characterization of an engineered multi-domain protein: kinetic versus equilibrium intermediates. Biochemistry-US. 2005, 第 1 作者
[37] Zheng Zhou. The folding pathway of Rd-apocyt b562: Demonstration of the on-pathway nature of a hidden intermediate and characterization of the rate-limiting transition state by protein. J. Mol. Biol.. 2005, 第 1 作者
[38] 周政, 李敏, 梁宋平. 虎纹捕鸟蛛毒素—ⅠcDNA克隆及序列. 中国生物化学与分子生物学报[J]. 2001, 第 1 作者17(1): 51, http://lib.cqvip.com/Qikan/Article/Detail?id=4866256.
[39] 周政, 李敏. 介绍一种高效，简单的DNA沉淀法. 生命的化学[J]. 1999, 第 1 作者19(1): 32, http://lib.cqvip.com/Qikan/Article/Detail?id=3433253.
[40] 周政, 李敏. 对ZnCl2沉淀DNA的方法的优化. 生命科学研究[J]. 1998, 第 1 作者2(4): 305-308, http://lib.cqvip.com/Qikan/Article/Detail?id=3304984.

（1） 蛋白质折叠方法学：利用氢氘交换介导的蛋白质工程富集蛋白折叠中间体及利用核磁共振进行结构研究, Protein folding protocols: Population and structure determination of hidden folding intermediates by native-state hydrogen exchange-directed protein engineering and nuclear magnetic resonance, Humana Press, 2006-02, 第 3 作者

   

（ 1 ） 组蛋白变体结构及其参与的表观遗传调控机理研究, 主持, 国家级, 2012-01--2015-12
（ 2 ） 组蛋白二聚体的动态结构及其生物学功能研究, 主持, 国家级, 2013-01--2016-12
（ 3 ） 表观遗传信息建立与解读的分子基础, 参与, 国家级, 2015-01--2016-08
（ 4 ） 染色质结构及其调控, 参与, 国家级, 2016-01--2021-12
（ 5 ） 染色质重构复合物SRCAP/SWR1介导的H2A.Z染色质组装机制研究, 主持, 国家级, 2017-01--2020-12

（1）Preferential recognition of H2A.Z-H2B by Mps3 direct DNA double-strand breaks to nuclear periphery   2017-09-06
（2）Structural basis of H2A.Z recognition by SRCAP subunit YL1   2016-07-28
（3）Structural basis of H2A.Z recognition by SRCAP chromatin-remodeling subunit YL1   Zheng Zhou   2016-05-09
（4）Structural basis of H2A.Z recognition by its chaperones   2016-04-09
（5）Structural study of H2A.Z specific histone chaperone   2014-08-04
（6）Structural basis for recognition of centromere specific histone H3 variant by nonhistone Scm3   Zheng Zhou, Hanqiao Feng, Yawen Bai   2010-03-01

已指导学生

梁小平  博士研究生  071010-生物化学与分子生物学  

潘露  博士研究生  071010-生物化学与分子生物学  

王云云  博士研究生  071010-生物化学与分子生物学  

谢晓燕  博士研究生  071010-生物化学与分子生物学  

周驹俊  博士研究生  071010-生物化学与分子生物学  

吴飞  硕士研究生  085238-生物工程  

戴亚鑫  博士研究生  071010-生物化学与分子生物学  

黄艳  博士研究生  071010-生物化学与分子生物学  

现指导学生

黄莉  博士研究生  071010-生物化学与分子生物学  

周宁  博士研究生  071010-生物化学与分子生物学  

谌姣  博士研究生  071010-生物化学与分子生物学  

周英博  硕士研究生  077802-免疫学  

史刘歆  博士研究生  071010-生物化学与分子生物学  

王龙歌  博士研究生  071010-生物化学与分子生物学  

朱浩强  博士研究生  071010-生物化学与分子生物学