分泌蛋白质及外泌体在组织器官再生过程中调控机制研究

070302-分析化学

分泌蛋白及胞外囊泡的蛋白质组学分析，鹿茸再生过程中关键活性物质的研究

2006-09--2009-07   吉林大学   博士
2003-09--2006-07   吉林大学   硕士
1998-09--2002-07   吉林大学   本科

   

2014-07~2016-06,中科院大连化物所, 副研究员
2013-06~2014-07,中科院大连化物所, 助理研究员
2012-02~2013-08,中科院大连化物所, 博士后
2009-07~2011-10,吉林大学, 讲师

   

[1] 张丽华, 随志刚, 杨开广, 侯瑞, 袁辉明, 张玉奎. 一种外泌体的富集方法. CN: CN110411816A, 2019-11-05.
[2] 张丽华, 袁辉明, 随志刚, 杨开广, 张玉奎. 一种离体体液中蛋白质的预处理方法. CN: CN108088727A, 2018-05-29.
[3] 张丽华, 陈远波, 邓楠, 吴琪, 梁振, 随志刚, 李云峰, 张晓丹, 杨开广, 张玉奎. 一种尿液蛋白质组学样品预处理方法及应用. CN: CN107561164A, 2018-01-09.
[4] 张丽华, 随志刚, 张玉奎, 杨开广. 一种鹿茸组织细胞外基质的制备方法. CN: CN106854636A, 2017-06-16.
[5] 张丽华, 翁叶靖, 随志刚, 杨开广, 张玉奎. 一种含血清培养液中分泌蛋白的样品预处理方法. CN: CN106324249A, 2017-01-11.
[6] 张丽华, 翁叶靖, 蒋好, 随志刚, 杨开广, 张玉奎. 一种特殊糖肽富集和鉴定的方法. CN: CN105277398A, 2016-01-27.
[7] 张丽华, 方菲, 赵群, 随志刚, 杨开广, 张玉奎. 一种细胞膜蛋白质富集纯化方法. CN: CN104628810A, 2015-05-20.
[8] 张丽华, 随志刚, 杨开广, 张玉奎. 一种可保持蛋白质活性的软骨组织分级处理方法. CN: CN103776667A, 2014-05-07.
[9] 张丽华, 随志刚, 张晓丹, 张玉奎. 一种可以彻底去除鹿茸中血液残留的清洗方法. CN: CN103769393A, 2014-05-07.
[10] 张丽华, 随志刚, 张玉奎. 一种可保持活性的蛋白质顺序提取方法. CN: CN103509081A, 2014-01-15.

   

[1] He, Wang, Yingyun, He, Bo, Jiang, Zhigang, Sui, Huiming, Yuan, Baofeng, Zhao, Zhen, Liang, Lihua, Zhang, Yukui, Zhang. Widespread protein N-phosphorylation in organism revealed by SiO2@DpaZn beads based mild-acidic enrichment method. TALANTA[J]. 2023, 251: http://dx.doi.org/10.1016/j.talanta.2022.123740.
[2] Liu, Jianhui, Zhou, Yuan, Hou, Xinhang, Liu, Chao, Zhao, Baofeng, Shan, Yichu, Sui, Zhigang, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. A1 Ions: Peptide-Specific and Intensity-Enhanced Fragment Ions for Accurate and Multiplexed Proteome Quantitation. ANALYTICAL CHEMISTRY[J]. 2022, 94(21): 7637-7646, http://dx.doi.org/10.1021/acs.analchem.2c00876.
[3] Chen, Lingfan, Shan, Yichu, Yang, Chao, Sui, Zhigang, Zhang, Xiaodan, Zhang, Lihua, Zhang, Yukui. Carboxypeptidase B-Assisted Charge-Based Fractional Diagonal Chromatography for Deep Screening of C-Terminome. ANALYTICAL CHEMISTRY[J]. 2020, 92(12): 8005-8009, http://dx.doi.org/10.1021/acs.analchem.0c00762.
[4] Li, Ting, Sui, Zhigang, Matsuno, Akira, Ten, Hirotomo, Oyama, Kenichi, Ito, Akihiro, Jiang, Hong, Ren, Xiaomin, Javed, Rabia, Zhang, Lihua, Ao, Qiang. Fabrication and Evaluation of a Xenogeneic Decellularized Nerve-Derived Material: Preclinical Studies of a New Strategy for Nerve Repair. NEUROTHERAPEUTICS[J]. 2020, 17(1): 356-370, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007487/.
[5] Deng, Nan, Chen, Yuanbo, Liang, Zhen, Bian, Yangyang, Wang, Bing, Sui, Zhigang, Zhang, Xiaodan, Yang, Kaiguang, Zhang, Lihua, Zhang, Yukui. Ampholine immobilized polymer microspheres for increasing coverage of human urinary proteome. TALANTA[J]. 2020, 215: http://dx.doi.org/10.1016/j.talanta.2020.120931.
[6] Sui, Zhigang, Sun, Hongmei, Weng, Yejing, Zhang, Xiaodan, Sun, Mingwei, Sun, Rui, Zhao, Baofeng, Liang, Zhen, Zhang, Yukui, Li, Chunyi, Zhang, Lihua. Quantitative proteomics analysis of deer antlerogenic periosteal cells reveals potential bioactive factors in velvet antlers. JOURNAL OF CHROMATOGRAPHY A[J]. 2020, 1609: http://dx.doi.org/10.1016/j.chroma.2019.460496.
[7] Sun, Hongmei, Sui, Zhigang, Wang, Datao, Ba, Hengxing, Zhao, Haiping, Zhang, Lihua, Li, Chunyi. Identification of interactive molecules between antler stem cells and dermal papilla cells using an in vitro co-culture system. JOURNAL OF MOLECULAR HISTOLOGY[J]. 2020, 51(1): 15-31, https://www.webofscience.com/wos/woscc/full-record/WOS:000518502900003.
[8] Hu, Yechen, Jiang, Bo, Weng, Yejing, Sui, Zhigang, Zhao, Baofeng, Chen, Yuanbo, Liu, Lukuan, Wu, Qiong, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. Bis(zinc(II)-dipicolylamine)-functionalized sub-2 mu m core-shell microspheres for the analysis of N-phosphoproteome. NATURE COMMUNICATIONS[J]. 2020, 11(1): https://www.webofscience.com/wos/woscc/full-record/WOS:000597327900001.
[9] Chen, Di, Zhao, Xinjie, Sui, Zhigang, Niu, Huan, Chen, Luonan, Hu, Cheng, Xuan, Qiuhui, Hou, Xuhong, Zhang, Rong, Zhou, Lina, Li, Yanli, Yuan, Huiming, Zhang, Yukui, Wu, Jiarui, Zhang, Lihua, Wu, Renan, Piao, HaiLong, Xu, Guowang, Jia, Weiping. A multi-omics investigation of the molecular characteristics and classification of six metabolic syndrome relevant diseases. THERANOSTICS[J]. 2020, 10(5): 2029-2046, https://www.webofscience.com/wos/woscc/full-record/WOS:000508008300004.
[10] Li, Yang, Fang, Fei, Sun, Mingwei, Zhao, Qun, Hu, Yechen, Sui, Zhigang, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. Ionic liquid-assisted protein extraction method for plant phosphoproteome analysis. TALANTA[J]. 2020, 213: http://dx.doi.org/10.1016/j.talanta.2020.120848.
[11] Liu, Jianhui, Zhou, Yuan, Shan, Yichu, Zhao, Baofeng, Hu, Yechen, Sui, Zhigang, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. A Multiplex Fragment-Ion-Based Method for Accurate Proteome Quantification. ANALYTICAL CHEMISTRY[J]. 2019, 91(6): 3921-3928, http://www.corc.org.cn/handle/1471x/2372628.
[12] Wu Qiong, Yuan Huiming, Weng Yejing, Sui Zhigang, Zhang Xiaodan, Hu Yechen, Lang Zhen, Zhang Lihua, Zhang Yukui. A novel method for the selective enrichment of persulfidated peptides based on iodoacetic acid functionalized dendrimer. CHINESE JOURNAL OF CHROMATOGRAPHY[J]. 2019, 37(8): 836-844, 
[13] Hou, Rui, Li, Yilan, Sui, Zhigang, Yuan, Huiming, Yang, Kaiguang, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. Advances in exosome isolation methods and their applications in proteomic analysis of biological samples. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2019, 411(21): 5351-5361, 
[14] Liu, Xinlu, Weng, Yejing, Liu, Peng, Sui, Zhigang, Zhou, Lei, Huang, Yinpeng, Zhang, Lihua, Zhang, Yukui, Tan, Xiaodong. Identification of PGAM1 as a putative therapeutic target for pancreatic ductal adenocarcinoma metastasis using quantitative proteomics. ONCOTARGETS AND THERAPY[J]. 2018, 11: 3345-3357, https://doaj.org/article/5f99881a29bb444384ba85616b500965.
[15] Li, Na, Sui, Zhigang, Liu, Yong, Wang, Dandan, Ge, Guangbo, Yang, Ling. A fast screening model for drug permeability assessment based on native small intestinal extracellular matrix. RSC ADVANCES[J]. 2018, 8(60): 34514-34524, http://www.corc.org.cn/handle/1471x/2373127.
[16] Zhao, Qun, Fang, Fei, Shan, Yichu, Sui, Zhigang, Zhao, Baofeng, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. In-Depth Proteome Coverage by Improving Efficiency for Membrane Proteome Analysis. ANALYTICAL CHEMISTRY[J]. 2017, 89(10): 5179-5185, http://dx.doi.org/10.1021/acs.analchem.6b04232.
[17] Zhang, Shen, Shan, Yichu, Zhang, Shurong, Sui, Zhigang, Zhang, Lihua, Liang, Zhen, Zhang, Yukui. NIPTL-Novo: Non-isobaric peptide termini labeling assisted peptide de novo sequencing. JOURNAL OF PROTEOMICS[J]. 2017, 154: 40-48, http://cas-ir.dicp.ac.cn/handle/321008/169380.
[18] Sui, Zhigang, Weng, Yejing, Zhao, Qun, Deng, Nan, Fang, Fei, Zhu, Xudong, Shan, Yichu, Zhang, Lihua, Zhang, Yukui. Ionic liquid-based method for direct proteome characterization of velvet antler cartilage. TALANTA[J]. 2016, 161: 541-546, http://cas-ir.dicp.ac.cn/handle/321008/169798.
[19] Weng Yejing, Sui Zhigang, Zhang Lihua, Zhang Yukui. Recent advances on exosomes isolation methods and proteomic analysis. CHINESE JOURNAL OF CHROMATOGRAPHY[J]. 2016, 34(12): 1131-1136, 
[20] Zhang, Shen, Chen, Lingfan, Shan, Yichu, Sui, Zhigang, Wu, Qi, Zhang, Lihua, Liang, Zhen, Zhang, Yukui. Pseudo isobaric peptide termini labelling for relative proteome quantification by SWATH MS acquisition. ANALYST[J]. 2016, 141(16): 4912-4918, http://cas-ir.dicp.ac.cn/handle/321008/148443.
[21] Weng, Yejing, Sui, Zhigang, Shan, Yichu, Hu, Yechen, Chen, Yuanbo, Zhang, Lihua, Zhang, Yukui. Effective isolation of exosomes with polyethylene glycol from cell culture supernatant for in-depth proteome profiling. ANALYST[J]. 2016, 141(15): 4640-4646, http://cas-ir.dicp.ac.cn/handle/321008/148425.
[22] Chen, Lingfan, Shan, Yichu, Weng, Yejing, Yuan, Huiming, Zhang, Shen, Fan, Runlong, Sui, Zhigang, Zhang, Xiaodan, Zhang, Lihua, Zhang, Yukui. Depletion of internal peptides by site-selective blocking, phosphate labeling, and TiO2 adsorption for in-depth analysis of C-terminome. ANALYTICAL AND BIOANALYTICAL CHEMISTRY[J]. 2016, 408(14): 3867-3874, http://cas-ir.dicp.ac.cn/handle/321008/148428.
[23] Fang, Fei, Zhao, Qun, Sui, Zhigang, Liang, Yu, Jiang, Hao, Yang, Kaiguang, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. Glycan Moieties as Bait to Fish Plasma Membrane Proteins. ANALYTICAL CHEMISTRY[J]. 2016, 88(10): 5065-5071, http://dx.doi.org/10.1021/acs.analchem.6b01082.
[24] Weng, Yejing, Sui, Zhigang, Shan, Yichu, Jiang, Hao, Zhou, Yuan, Zhu, Xudong, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. In-Depth Proteomic Quantification of Cell Secretome in Serum-Containing Conditioned Medium. ANALYTICAL CHEMISTRY[J]. 2016, 88(9): 4971-4978, http://cas-ir.dicp.ac.cn/handle/321008/148413.
[25] 翁叶靖, 随志刚, 张丽华, 张玉奎. 外泌体分离及其蛋白质组学分析的研究进展. 色谱[J]. 2016, 34(12): 1131-1136, http://lib.cqvip.com/Qikan/Article/Detail?id=672065920.
[26] Zhang, Shen, Wu, Qi, Shan, Yichu, Zhao, Qun, Zhao, Baofeng, Weng, Yejing, Sui, Zhigang, Zhang, Lihua, Zhang, Yukui. Fast MS/MS acquisition without dynamic exclusion enables precise and accurate quantification of proteome by MS/MS fragment intensity. SCIENTIFIC REPORTS[J]. 2016, 6: http://cas-ir.dicp.ac.cn/handle/321008/148414.
[27] Liu, Peng, Weng, Yejing, Sui, Zhigang, Wu, Yunhao, Meng, Xiangli, Wu, Mengwei, Jin, Haoyi, Tan, Xiaodong, Zhang, Lihua, Zhang, Yukui. Quantitative secretomic analysis of pancreatic cancer cells in serum-containing conditioned medium. SCIENTIFIC REPORTS[J]. 2016, 6: http://cas-ir.dicp.ac.cn/handle/321008/169724.
[28] Chen, Lingfan, Shan, Yichu, Weng, Yejing, Sui, Zhigang, Zhang, Xiaodan, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. Hydrophobic Tagging-Assisted N-Termini Enrichment for In-Depth N-Terminome Analysis. ANALYTICAL CHEMISTRY[J]. 2016, 88(17): 8390-8395, http://cas-ir.dicp.ac.cn/handle/321008/170254.
[29] Zhigang Sui. eicoplanin bonded sub-2 mu m superficially porous particles for enantioseparation of native amino acids. Journal of Pharmaceutical and Biomedical Analysis. 2015, 
[30] Min, Yi, Sui, Zhigang, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. Teicoplanin bonded sub-2 mu m superficially porous particles for enantioseparation of native amino acids. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS[J]. 2015, 114(1): 247-253, http://159.226.238.44/handle/321008/143708.
[31] Weng, Yejing, Jiang, Bo, Yang, Kaiguang, Sui, Zhigang, Zhang, Lihua, Zhang, Yukui. Polyethyleneimine-modified graphene oxide nanocomposites for effective protein functionalization. NANOSCALE[J]. 2015, 7(34): 14284-14291, http://cas-ir.dicp.ac.cn/handle/321008/143725.
[32] Zhang, Shen, Wu, Qi, Shan, Yichu, Sui, Zhigang, Zhang, Lihua, Zhang, Yukui. A paired ions scoring algorithm based on Morpheus for simultaneous identification and quantification of proteome samples prepared by isobaric peptide termini labeling strategies. PROTEOMICS[J]. 2015, 15(11): 1781-1788, http://159.226.238.44/handle/321008/143710.
[33] Weng, Yejing, Sui, Zhigang, Jiang, Hao, Shan, Yichu, Chen, Lingfan, Zhang, Shen, Zhang, Lihua, Zhang, Yukui. Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. SCIENTIFIC REPORTS[J]. 2015, 5(1): http://159.226.238.44/handle/321008/143716.
[34] Deng, Nan, Zhu, Guijie, Chen, Yuanbo, Wu, Qi, Liang, Zhen, Sui, Zhigang, Zhang, Lihua, Yang, Kaiguang, Zhang, Yukui. Decrease of dynamic range of proteins in human plasma by ampholine immobilized polymer microspheres. ANALYTICA CHIMICA ACTA[J]. 2014, 826: 43-50, http://dx.doi.org/10.1016/j.aca.2014.04.004.
[35] Sui, Zhigang, Zhang, Lihua, Huo, Yushu, Zhang, Yukui. Bioactive components of velvet antlers and their pharmacological properties. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS[J]. 2014, 87(1): 229-240, http://dx.doi.org/10.1016/j.jpba.2013.07.044.
[36] Li, Na, Wang, Dandan, Sui, Zhigang, Qi, Xiaoyi, Ji, Liyun, Wang, Xiuli, Yang, Ling. Development of an Improved Three-Dimensional In Vitro Intestinal Mucosa Model for Drug Absorption Evaluation. TISSUE ENGINEERING PART C-METHODS[J]. 2013, 19(9): 708-719, http://www.irgrid.ac.cn/handle/1471x/858688.
[37] 赵群, 方菲, 随志刚, 张丽华, 张玉奎. BIPHASIC MICROREACTOR FOR EFFICIENT MEMBRANE PROTEIN ANALYSIS OF RARE CELL SAMPLE. the 15th beijing conference and exhibition on instrumental analysis (bceia2013). 2013, 1147, http://159.226.238.44/handle/321008/120117.
[38] 翁叶靖, 曲焱焱, 张丽华, 袁辉明, 蒋好, 随志刚, 张玉奎. An integrated platform for quantitative glycoproteome analysis. 22nd international symposium on glycoconjugates. 2013, 365, http://159.226.238.44/handle/321008/120131.
[39] Zhao, Qun, Liang, Yu, Yuan, Huiming, Sui, Zhigang, Wu, Qi, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. Biphasic Microreactor for Efficient Membrane Protein Pretreatment with a Combination of Formic Acid Assisted Solubilization, On-Column pH Adjustment, Reduction, Alkylation, and Tryptic Digestion. ANALYTICAL CHEMISTRY[J]. 2013, 85(18): 8507-8512, http://www.irgrid.ac.cn/handle/1471x/858574.
[40] 邓楠, 陈远波, 吴慈, 梁振, 梁玉, 随志刚, 张丽华, 张玉奎. DECREASE OF DYNAMIC RANGE OF PROTEINS IN HUMAN PLASMA BY AMPHOLINE IMMOBILIZED POLYMER MICROSPHERES. The 15th beijing conference and exhibition on instrumental analysis (bceia2013). 2013, 1048, http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000335452300006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=3a85505900f77cc629623c3f2907beab.
[41] Sui, Zhigang, Yuan, Huiming, Liang, Zhen, Zhao, Qun, Wu, Qi, Xia, Simin, Zhang, Lihua, Huo, Yushu, Zhang, Yukui. An activity-maintaining sequential protein extraction method for bioactive assay and proteome analysis of velvet antlers. TALANTA[J]. 2013, 107: 189-194, http://dx.doi.org/10.1016/j.talanta.2013.01.015.
[42] Sui, Zhigang, Li, Na, Liu, Zhiqiang, Yan, Jun, Liu, Zhongying. Metabolite profile analysis of aconitine in rabbit stomach after oral administration by liquid chromatography/electrospray ionization/multiple- stage tandem mass spectrometry. XENOBIOTICA[J]. 2013, 43(7): 628-635, http://159.226.238.44/handle/321008/137609.
[43] 随志刚, 赵群, 邓楠, 蒋好, 梁振, 张丽华, 张玉奎. A new strategy for studying proteome of velvet antler cartilage. 40th international symposium on high performance liquid phase separations and related techiniques 2013. 2013, 134, http://159.226.238.44/handle/321008/120124.
[44] 随志刚, 赵群, 吴琪, 夏思敏, 张丽华, 霍玉书, 张玉奎. Development of Sequential Protein Extraction Method for Bioactive and Proteome Analysis of Velvet Antlers. ASIA OCEANIA HUMAN PROTEOME ORGANIZATION 6TH CONGRESS. 2012, 284, http://159.226.238.44/handle/321008/117090.
[45] Deng, Nan, Liang, Zhen, Liang, Yu, Sui, Zhigang, Zhang, Liyuan, Wu, Qi, Yang, Kaiguang, Zhang, Lihua, Zhang, Yukui. Aptamer Modified Organic-Inorganic Hybrid Silica Monolithic Capillary Columns for Highly Selective Recognition of Thrombin. ANALYTICAL CHEMISTRY[J]. 2012, 84(23): 10186-10190, http://159.226.238.44/handle/321008/142981.
[46] Li, Na, Tsao, Rong, Sui, Zhigang, Ma, Jingwei, Liu, Zhiqiang, Liu, Zhongying. Intestinal Transport of Pure Diester-type Alkaloids from an Aconite Extract across the Caco-2 Cell Monolayer Model. PLANTA MEDICA[J]. 2012, 78(7): 692-697, http://www.irgrid.ac.cn/handle/1471x/721811.
[47] 张玉奎, 随志刚, 张丽华, 霍玉书. An Activity-maintaining Protein Extraction Method for Bioactive Assay and Proteome Analysis of Velvet Antlers. 2012 IOCD 天然功能分子国际研讨会. 2012, 15, http://159.226.238.44/handle/321008/117069.
[48] 随志刚, 袁辉明, 赵群, 吴琪, 高亮, 梁振, 张丽华, 霍玉书, 张玉奎. A sequential protein extraction strategy for analysis of deer antler proteome and biological activity. 37TH INTERNATIONAL SYMPOSIUM ON HIGH-PERFORMANCE LIQUID PHASE SEPARATIONS AND RELATED TECHNIQUES. 2011, 463, http://159.226.238.44/handle/321008/117404.

   

（ 1 ） 蛋白质组末端肽的深度覆盖定性和高准确度定量新技术研究, 参与, 国家级, 2015-01--2018-12
（ 2 ） 功能蛋白质组学样品制备方法的研究及在鹿茸活性蛋白质筛选中的应用, 主持, 国家级, 2014-01--2016-12