Developed a   series of prokaryotic expression system for the production of industrial enzymes

Established   a series of high efficiency E. coli   expression systems using metabolic regulation

Constructed a interspecific   plasmid transfer system in Bacillus   species with the counter-selectable marker mazF

Designed various high   copy and high stable E.   coli-B.subtilis shuttle plasmids

Applied programmable   nucleases (CRISPR/Cas9) for genome editing in Bacillus sp

Discovered,   characterized and engineered various industrial enzymes such as pullulanase, mannase,   catalase, pectase, and so on

Revealed the properties   of CBM68 in the N-terminal of pullulanase

Developed an acidic xylanase   from the metagenomic DNA of cow dung compost and used it to produce xylooligosaccharides   from corncob

Designed a fluorescent   protein marker to reveal the degradation mechanism of corncob hemicellulose

Developed a thermostable   and alkaline xylanase using the combination of ep-PCR and directed mutation

Revealed properties of   the xylan‑binding domain and linker sequence of xylanase XynG1‑1

Firstly accomplished the   overexpression of xylanase in Bacillus   megatherium MS941

PhD student			2009-2012
	College of   Biotechnology Tianjin University of Science and Technology, Tianjin, China
	Advisor: Professor Fuping   Lu

   

Postdoctoral Research Assistant

2012-2014

Biomass   engineering center

Chinese Academy of Agricultural Engineering, Beijing, China

Assistant Professor 

2014-2018

Tianjin institute of industrial biotechnology, Chinese academy   sciences, Tianjin, China

 

Associate   Professor

2018-2025

Tianjin institute of industrial biotechnology, Chinese academy   sciences, Tianjin, China

Professor-level Senior Engineer

2025-

Tianjin institute of industrial biotechnology, Chinese academy   sciences, Tianjin, China

   

1. Liang H#, Dong W#, Fu X, Zheng H*, Li M*, Bai W*. High-efficiency secretory expression of polyethylene terephthalate hydrolases in Bacillus amyloliquefaciens 205 and complete degradation of PET by its crude enzyme. Int J Biol Macromol. 2025, 330(Pt 2):148111.

2. Qingfang Meng#, Dexin Wang#, Xiaoping Fu, Weitao Geng*, Hongchen Zheng*, and Wenqin Bai*. Converting Bacillus subtilis 168 to a Synthetic Methylotroph by Combinatorial Metabolic Regulation Strategies. Journal of Agricultural and Food Chemistry. 2025, 73(8): 4755-4763.

3. Zhao Xingya, Fu Xiaoping, Zhen Jie, Yang Jun, Zheng Hongchen*, Bai Wenqin*, Song Hui. BsuMI regulates DNA transformation in Bacillus subtilis besides the defense system and the constructed strain with BsuMI absence is applicable as a universal transformation platform for wild-type Bacillus. Microb Cell Fact. 2024, 23: 225.

4. Yifan Yang#, Xiaoping Fu#, Xingya Zhao, Jianyong Xu, Yihan Liu*, Hongchen Zheng*, Wenqin Bai*, Hui Song. Overexpression of a Thermostable α-Amylase through Genome Integration in Bacillus subtilis. Fermentation-Basel. 2023, 9(2):139.

5. Hongchen zheng#,*, Wenju Shu#, Xiaoping Fu#, Xingya Zhao, Shibin Yang, Jianyong Xu*, Hui Song*, Yanhe M. A pyruvate-centered metabolic regulation mechanism for the enhanced expression of exogenous genes in Escherichia coli. Int J Biol Macromol. 2022, 203:58-66.

6. Jie Zhen#, Hongchen Zheng#,*, Xingya Zhao#, Xiaoping Fu, Shibin Yang, Jianyong Xu*, Hui Song*, Yanhe Ma. Regulate the hydrophobic motif to enhance the non-classical secretory expression of Pullulanase PulA in Bacillus subtilis. Int J Biol Macromol. 2021, 193: 238-246.

7. ShiBin Yang#, HongChen Zheng#,*, JianYong Xu, XingYa Zhao, WenJu Shu, XiangMing Li, Hui Song*, YanHe Ma*. A new biotransformation mode of zearalenone identified in Bacillus subtilis Y816 revealing a novel ZEN conjugate. Journal of Agricultural and Food Chemistry. 2021, 69(26): 7409-7419.

8. Zhao X#, Zheng H#,*, Zhen J, Shu W, Yang S, Xu J, Song H*, Ma Y*. Multiplex genetic engineering improves endogenous expression of mesophilic α-amylase gene in a wild strain Bacillus amyloliquefaciens 205. Int J Biol Macromol. 2020, 165(Pt A): 609-618.

9. Zhao X#, Xu J#, Tan M#, Zhen J, Shu W, Yang S, Zheng H*, Ma Y*, Song H*. High copy number and highly stable Escherichia coli-Bacillus subtilis shuttle plasmids based on pWB980. Microb Cell Fact. 2020, 19(1): 25.

10. Zeng Y#, Xu J#, Fu X, Tan M, Liu F, Zheng H*, Song H*. Effects of different carbohydrate-binding modules on the enzymatic properties of pullulanase. Int J Biol Macromol. 2019, 137:973-981.

11. Zheng H#, Yu Z#, Shu W#, Fu X, Zhao X, Yang S, Tan M, Xu J, Liu Y*, Song H*. Ethanol effects on the overexpression of heterologous catalase in Escherichia coli BL21 (DE3). Appl Microbiol Biotechnol. 2019, 103(3): 1441-1453.

12. Zhao X#, Xu J#, Tan M, Yu Z, Yang S, Zheng H*, Song H*. Construction of a plasmid interspecific transfer system in Bacillus species with the counter-selectable marker mazF. J Ind Microbiol Biotechnol. 2018, 45(6):417-428.

13. Yu Z#, Zheng HC#, Zhao X, Li S, Xu J, Song H*. High level extracellular production of a recombinant alkaline catalase in E. coli BL21 under ethanol stress and its application in hydrogen peroxide removal after cotton fabrics bleaching. Bioresour Technol. 2016, 214: 303-310.

14. Zheng Hongchen#, Yihan Liu#, Xiaoguang Liu, Yang Han, Jianling Wang, Fuping Lu*. Overexpression of a Paenibacillus campinasensis xylanase in Bacillus megaterium and its applications to biobleaching of cotton stalk pulp and saccharification of recycled paper sludge. Bioresource Technology. 2012, 125: 182-187.

3. Hongchen Zheng, “A new strategy for improving exogenous gene   expression in E. coli”, YUST-TIB.CAS Young and middle-aged   scholars academic BBS, Tianjin, China, November 6, 2018 (Talk)

2. Hongchen Zheng, “Mechanism by which ethanol promotes exogenous gene expression in E. coli”, The 8th China   fermentation engineering academic seminar in 2018, Wuxi, China, October 10-12,   2018 (Talk)

1. Hongchen Zheng, “Purification and properties of fibrinolytic enzymes from N.sitophila”, Annual meeting of the Chinese academy of agricultural engineering, Qiqihaer, China, July 26-27, 2008 (Excellent graduate thesis award)

已指导学生

赵博华  硕士研究生  086000-生物与医药