Developed a   series of prokaryotic expression system for the production of industrial enzymes

Established   a series of high efficiency E. coli   expression systems using metabolic regulation

Constructed a interspecific   plasmid transfer system in Bacillus   species with the counter-selectable marker mazF

Designed various high   copy and high stable E.   coli-B.subtilis shuttle plasmids

Applied programmable   nucleases (CRISPR/Cas9) for genome editing in Bacillus sp

Discovered,   characterized and engineered various industrial enzymes such as pullulanase, mannase,   catalase, pectase, and so on

Revealed the properties   of CBM68 in the N-terminal of pullulanase

Developed an acidic xylanase   from the metagenomic DNA of cow dung compost and used it to produce xylooligosaccharides   from corncob

Designed a fluorescent   protein marker to reveal the degradation mechanism of corncob hemicellulose

Developed a thermostable   and alkaline xylanase using the combination of ep-PCR and directed mutation

Revealed properties of   the xylan‑binding domain and linker sequence of xylanase XynG1‑1

Firstly accomplished the   overexpression of xylanase in Bacillus   megatherium MS941

PhD student

2009-2012

College of   Biotechnology

Tianjin University of Science and Technology, Tianjin, China

Advisor: Professor Fuping   Lu

Postdoctoral Research Assistant

2012-2014

Biomass   engineering center

Chinese Academy of Agricultural Engineering, Beijing, China

Associate   Professor

2018-present

Tianjin institute of industrial biotechnology, Chinese academy   sciences, Tianjin, China

Assistant Professor

2014-2018

Tianjin institute of industrial biotechnology, Chinese academy   sciences, Tianjin, China

12.

Zheng   H^#, Yu Z^#, Shu W^#, Fu   X, Zhao X, Yang S, Tan M, Xu J, Liu Y^*, Song H^*. Ethanol   effects on the overexpression of heterologous catalase in Escherichia coli BL21 (DE3). Appl   Microbiol Biotechnol. 2019, 103(3): 1441-1453.

11.

10.

Zeng Y^#, Zheng H^#, Shen Y, Xu J, Tan M, Liu F, Song H^*.   Identification and analysis of binding residues in the CBM68 of pullulanase   PulA from Anoxybacillus sp. LM18-11.   J.   Biosci. Bioeng. 2019, 127(1): 8-15.

Zhao X^#, Xu J^#, Tan   M, Yu Z, Yang S, Zheng H^*,   Song H^*. Construction of a plasmid interspecific transfer system   in Bacillus species with the   counter-selectable marker mazF. J Ind Microbiol Biotechnol. 2018, 45(6):417-428.

9.

Yu Z^#, Zheng HC^#, Zhao X, Li S, Xu J, Song H^*.   High level extracellular production of a recombinant alkaline catalase in E. coli BL21 under ethanol stress and   its application in hydrogen peroxide removal after cotton fabrics bleaching. Bioresour   Technol. 2016, 214: 303-310.

8.

Zheng   HC, Yu Z, Fu X, Li S, Xu   J, Song H^*, Ma Y. High level extracellular production of a   truncated alkaline β-mannanase from alkaliphilic Bacillus sp. N16-5 in Escherichia   coli by the optimization of induction condition and fed-batch   fermentation. J Ind Microbiol Biotechnol. 2016, 43(7):977-87.

7.

Sun MZ^#, Zheng HC^#, Meng LC, Sun JS, Song H, Bao YJ, Pei HS,   Yan Z, Zhang XQ, Zhang JS, Liu YH, Lu FP^*. Direct cloning,   expression of a thermostable xylanase gene from the metagenomic DNA of cow   dung compost and enzymatic production of xylooligosaccharides from corncob.   Biotechnol   Lett. 2015, 37(9): 1877-86.

6.

Li SF, Xu JY, Bao YJ, Zheng HC, Song H^*.   Structure and sequence analysis-based engineering of pullulanase from Anoxybacillus sp. LM18-11 for improved   thermostability. J Biotechnol. 2015, 210:8-14.

5.

Liu Y, Huang L, Li W, Guo W, Zheng HC, Wang J, Lu F^*. Studies   on properties of the xylan‑binding domain and linker sequence of xylanase   XynG1‑1 from Paenibacillus   campinasensis G1‑1. J Ind Microbiol Biotechnol. 2015,   42(12): 1591-9.

4.

Zheng   Hongchen^#, Yihan Liu^#, Mingzhe Sun,   Yang Han, Jianling Wang, Junshe Sun, Fuping Lu^*. Improvement of alkalistability   and thermostability of Paenibacillus   campinasensis Family-11 xylanase by directed evolution and   site-directed mutagenesis. J Ind Microbiol Biotechnol. 2014,   41(1): 153-62.

3.

Zheng Hongchen, Ming-zhe Sun, Ling-cai   Meng, Hai-sheng Pei, Xiu-qing Zhang, Zheng Yan, Wen-hui Zeng, Jing-sheng   Zhang, Jin-rong Hu, Fu-ping Lu, Jun-she Sun^*. Purification and Characterization of a   Thermostable Xylanase from Paenibacillus   sp. NF1 and its Application in Xylooligosaccharides Production.   Journal   of Microbiology and Biotechnology. 2014, 24(4): 489-496.

2.

Zheng   Hongchen^#, Yihan Liu^#, Xiaoguang Liu,   Yang Han, Jianling Wang, Fuping Lu^*. Overexpression of a Paenibacillus campinasensis xylanase   in Bacillus megaterium and its   applications to biobleaching of cotton stalk pulp and saccharification of   recycled paper sludge. Bioresource Technology. 2012, 125:   182-187.

1.

Zheng Hongchen^#, Yihan Liu^#, Xiaoguang   Liu, Jianling Wang, Ying Han, Fuping Lu^*. Isolation, Purification   and Characterization of a Thermostable   Xylanase from a Novel Strain Paenibacillus campinasensis G1-1. Journal of Microbiology and   Biotechnology. 2012, 22(7): 930-938.

3. Hongchen Zheng, “A new strategy for improving exogenous gene   expression in E. coli”, YUST-TIB.CAS Young and middle-aged   scholars academic BBS, Tianjin, China, November 6, 2018 (Talk)

2. Hongchen Zheng, “Mechanism by which ethanol promotes exogenous gene expression in E. coli”, The 8th China   fermentation engineering academic seminar in 2018, Wuxi, China, October 10-12,   2018 (Talk)