膜蛋白质样品预处理；蛋白质定性与定量研究； 动态蛋白质相互作用组研究

   

070302-分析化学

膜蛋白质样品预处理
蛋白质定性与定量分析
动态蛋白质相互作用组

2008-09--2014-05   中国科学院大学；中国科学院大连化学物理研究所   博士
2004-09--2008-07   西北大学   学士

博士

理学博士

   

2022-07~现在, 中国科学院大连化学物理研究所, 研究员
2016-06~2022-07,中国科学院大连化学物理研究所, 副研究员
2014-07~2016-06,中国科学院大连化学物理研究所, 助理研究员

   

（ 1 ） 一种单端交联肽去除方法及其在蛋白质复合物交联位点分析中的应用, 发明专利, 2021, 第 3 作者, 专利号: CN112986569A

（ 2 ） 一种多功能交联剂及其制备方法和应用, 发明专利, 2021, 第 3 作者, 专利号: CN112979674A

（ 3 ） 基于糖苷键质谱可碎裂型化学交联剂的分析方法及应用, 发明专利, 2021, 第 3 作者, 专利号: CN109900814B

（ 4 ） 一种用于生物样本的高能量紫外交联仪, 发明专利, 2021, 第 3 作者, 专利号: CN112904013A

（ 5 ） 一种交联肽段定性定量分析方法, 发明专利, 2021, 第 4 作者, 专利号: CN111208299B

（ 6 ） 基于化学交联质谱解析的质膜蛋白质相互作用的鉴定方法, 发明专利, 2020, 第 3 作者, 专利号: CN111220679A

（ 7 ） 一种化学交联剂及其制备和应用, 发明专利, 2020, 第 3 作者, 专利号: CN111205213A

（ 8 ） 一种基于糖苷键质谱可碎裂型化学交联剂的分析方法及应用, 发明专利, 2019, 第 3 作者, 专利号: CN109900814A

（ 9 ） 一种糖苷键质谱碎裂型化学交联剂及其制备方法, 发明专利, 2019, 第 3 作者, 专利号: CN109897078A

（ 10 ） 一种交联肽段富集方法及其在蛋白质相互作用研究中的应用, 发明专利, 2019, 第 3 作者, 专利号: CN109839303A

（ 11 ） 一种蛋白质复合物交联信息深度覆盖的分析方法, 发明专利, 2019, 第 3 作者, 专利号: CN109425647A

（ 12 ） 一种基于数据依赖性质谱扫描模式的谱图库建立方法及其应用, 发明专利, 2018, 第 3 作者, 专利号: CN108072728A

（ 13 ） 一种用于研究蛋白质结构或蛋白质相互作用的分析方法, 专利授权, 2017, 第 3 作者, 专利号: CN107525842A

（ 14 ） 一种血液透析器上吸附蛋白的分析方法, 发明专利, 2017, 第 4 作者, 专利号: CN107064523A

（ 15 ） 一种基于二级质谱的全离子监测定量方法, 专利授权, 2017, 第 2 作者, 专利号: CN106324115A

（ 16 ） 一种基于离子液体的蛋白质组样品快速预处理方法, 发明专利, 2017, 第 3 作者, 专利号: CN106323705A

（ 17 ） 一种基于快速扫描和无动态排除的质谱采集模式, 发明专利, 2017, 第 4 作者, 专利号: CN106324069A

（ 18 ） 样品溶液中离子液体的去除方法, 发明专利, 2015, 第 2 作者, 专利号: CN105203372A

（ 19 ） 一种糖蛋白质富集纯化方法, 发明专利, 2015, 第 3 作者, 专利号: CN104710506A

（ 20 ） 一种细胞膜蛋白质富集纯化方法, 发明专利, 2015, 第 3 作者, 专利号: CN104628810A

（ 21 ） 一种生物样品纯化的装置及其应用, 发明专利, 2014, 第 2 作者, 专利号: CN104236984A

（ 22 ） 溶液样品中含长烷基链的离子液体的去除方法, 发明专利, 2014, 第 2 作者, 专利号: CN104236983A

（ 23 ） 一种基于聚合物相反转自组装分子印迹微球材料及其应用, 发明专利, 2014, 第 6 作者, 专利号: CN104069836A

（ 24 ） 一种双通道SPE柱及其在定量蛋白质组学的应用, 发明专利, 2014, 第 3 作者, 专利号: CN104076114A

（ 25 ） 一种蛋白质样品原位快速预处理方法, 发明专利, 2014, 第 3 作者, 专利号: CN104075931A

（ 26 ） 双相柱膜蛋白质微反应器及其应用, 发明专利, 2014, 第 2 作者, 专利号: CN103665098A

   

[1] Pan, Hui, Zhao, Qun, Xia, Yu, Zhao, Baofeng, Jiang, Bo, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. Ligand guided in vivo crosslinking and affinity purification mass spectrometry for identifying membrane receptors of Tau. TALANTA[J]. 2025, 第 2 作者287: http://dx.doi.org/10.1016/j.talanta.2025.127655.
[2] Angew. Chem. Int. Ed. Engl.. 2025,   通讯作者  
[3] Adv. Sci.. 2025,   通讯作者  
[4] JACS Au. 2024, 第 2 作者
[5] Brief. Bioinform.. 2024,   通讯作者  
[6] Nat. Commun.. 2024,   通讯作者  
[7] Anal. Chem.. 2024,   通讯作者  
[8] Anal. Chem.. 2024,   通讯作者  
[9] Angew. Chem. Int. Ed. Engl.. 2023,   通讯作者  
[10] Feng, Huan, Zhao, Qun, Zhang, Beirong, Hu, Hang, Liu, Meng, Wu, Kaifeng, Li, Xiaosong, Zhang, Xin, Zhang, Lihua, Liu, Yu. Enabling Photo-Crosslinking and Photo-Sensitizing Properties for Synthetic Fluorescent Protein Chromophores. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION[J]. 2023, 第 2 作者62(2): http://dx.doi.org/10.1002/anie.202215215.
[11] Li, Xinwei, Chen, Yuwan, Jiang, Jing, Zhou, Wen, Gao, Hang, Zhao, Qun, Liang, Zhen, Cao, Xupeng, Yang, Kaiguang, Zhang, Lihua, Zhang, Yukui. Crosslinker Nanocarriers Delivery to Chloroplasts for In Vivo Mapping of Photosynthetic Membrane Protein Complexes in Living Chlamydomonas reinhardtii Cells. ANALYTICAL CHEMISTRY. 2023, 第 6 作者http://dx.doi.org/10.1021/acs.analchem.3c01009.
[12] Chen, Jing, Zhao, Qun, Gao, Hang, Zhao, Lili, Chu, Huiying, Shan, Yichu, Liang, Zhen, Zhang, Yukui, Zhang, Lihua. A Glycosidic-Bond-Based Mass-Spectrometry-Cleavable Cross-linker Enables In Vivo Cross-linking for Protein Complex Analysis. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION[J]. 2023, 第 2 作者  通讯作者  62(24): http://dx.doi.org/10.1002/anie.202212860.
[13] Chen, Yuwan, Zhou, Wen, Xia, Yufei, Zhang, Weijie, Zhao, Qun, Li, Xinwei, Gao, Hang, Liang, Zhen, Ma, Guanghui, Yang, Kaiguang, Zhang, Lihua, Zhang, Yukui. Targeted cross-linker delivery for the in situ mapping of protein conformations and interactions in mitochondria. NATURE COMMUNICATIONS[J]. 2023, 第 5 作者14(1): http://dx.doi.org/10.1038/s41467-023-39485-3.
[14] Angew. Chem. Int. Ed. Engl.. 2023,   通讯作者  
[15] Zhang, Zenghu, Li, Dehai, Xie, Ruize, Guo, Ruoyu, Nair, Shailesh, Han, Huan, Zhang, Guojian, Zhao, Qun, Zhang, Lihua, Jiao, Nianzhi, Zhang, Yongyu. Plastoquinone synthesis inhibition by tetrabromo biphenyldiol as a widespread algicidal mechanism of marine bacteria. ISME Journal[J]. 2023, 第 8 作者17(11): 1979-1992, http://dx.doi.org/10.1038/s41396-023-01510-0.
[16] Angew. Chem. Int. Ed. Engl.. 2023,   通讯作者  
[17] Shen Di, Jin WenHan, Zhao Qun, Wang MengDie, Zhang BeiRong, Feng Huan, Wan Wang, Bai YuLong, Lyu HaoChen, Sun JiaLu, Liu,Yu. Covalent Proteome Solvatochromic Proteome Stress Sensor Based on Schiff Base Reaction.. Anal. Chem[J]. 2022, 第 3 作者14143-14150, 
[18] 韩若楠, 赵丽丽, 安雨馨, 梁振, 赵群, 张丽华, 张玉奎. 基于镜像酶正交酶切的蛋白质复合物规模化精准分析新方法. 色谱[J]. 2022, 第 5 作者40(3): 224-233, http://lib.cqvip.com/Qikan/Article/Detail?id=7106660811.
[19] Wan, Wang, Huang, Yanan, Xia, Qiuxuan, Bai, Yulong, Chen, Yuwen, Jin, Wenhan, Wang, Mengdie, Shen, Di, Lyu, Haochen, Tang, Yuqi, Dong, Xuepeng, Gao, Zhenming, Zhao, Qun, Zhang, Lihua, Liu, Yu. Covalent Probes for Aggregated Protein Imaging via Michael Addition. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION[J]. 2021, 第 13 作者60(20): 11335-11343, http://dx.doi.org/10.1002/anie.202015988.
[20] 郑诗颖, 褚宏伟, 赵群, 赵宝锋, 张丽华, 张玉奎. 基于离子液体的蛋白质组样品制备新方法研究进展. 中国科学 : 化学[J]. 2021, 第 3 作者51(10): 1373-1381, https://www.sciengine.com/doi/10.1360/SSC-2021-0087.
[21] Zhao, Lili, Zhao, Qun, Shan, Yichu, Fang, Fei, Zhang, Weijie, Zhao, Baofeng, Li, Xiao, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. Smart Cutter: An Efficient Strategy for Increasing the Coverage of Chemical Cross-Linking Analysis. ANALYTICAL CHEMISTRY[J]. 2020, 第 2 作者  通讯作者  92(1): 1097-1105, http://dx.doi.org/10.1021/acs.analchem.9b04161.
[22] Fang, Fei, Zhao, Qun, Chu, Huiying, Liu, Mingwei, Zhao, Baofeng, Liang, Zhen, Zhang, Lihua, Li, Guohui, Wang, Liming, Qin, Jun, Zhang, Yukui. Molecular Dynamics Simulation-assisted Ionic Liquid Screening for Deep Coverage Proteome Analysis. MOLECULAR & CELLULAR PROTEOMICS[J]. 2020, 第 2 作者19(10): 1724-1737, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015004/.
[23] Li, Yang, Fang, Fei, Sun, Mingwei, Zhao, Qun, Hu, Yechen, Sui, Zhigang, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. Ionic liquid-assisted protein extraction method for plant phosphoproteome analysis. TALANTA[J]. 2020, 第 4 作者  通讯作者  213: http://dx.doi.org/10.1016/j.talanta.2020.120848.
[24] Ye, ShangXiang, Gong, Zhou, Yang, Ju, An, YuXin, Liu, Zhu, Zhao, Qun, Lescop, Ewen, Dong, Xu, Tang, Chun. Ubiquitin is double-phosphorylated by PINK1 for enhanced pH-sensitivity of conformational switch. PROTEIN & CELL[J]. 2019, 第 6 作者10(12): 908-913, http://lib.cqvip.com/Qikan/Article/Detail?id=7100635736.
[25] Wu, Ci, Liang, Yu, Zhu, Xudong, Zhao, Qun, Fang, Fei, Zhang, Xiaodan, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. Macro-mesoporous organosilica monoliths with bridged-ethylene and terminal-vinyl: High-density click functionalization for chromatographic separation. ANALYTICA CHIMICA ACTA[J]. 2018, 第 4 作者1038: 198-205, http://cas-ir.dicp.ac.cn/handle/321008/166982.
[26] 赵群, 张丽华, 张玉奎. 蛋白质组学技术前沿进展. 应用化学[J]. 2018, 第 1 作者35(9): 977-983, http://yyhx.ciac.jl.cn/CN/10.11944/j.issn.1000-0518.2018.09.180208.
[27] Zhao, Qun, Chu, Hongwei, Zhao, Baofeng, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. Advances of ionic liquids-based methods for protein analysis. TRAC-TRENDS IN ANALYTICAL CHEMISTRY[J]. 2018, 第 1 作者108: 239-246, http://cas-ir.dicp.ac.cn/handle/321008/166874.
[28] Qun Zhao, Hongwei Chu, Baofeng Zhao, Zhen Liang, Lihua Zhang, Yukui Zhang. Advances of ionic liquids-based methods for protein analysis. TRENDS IN ANALYTICAL CHEMISTRY. 2018, 第 1 作者108: 239-246, http://cas-ir.dicp.ac.cn/handle/321008/166874.
[29] Liu, Lukuan, Yang, Kaiguang, Zhu, Xudong, Liang, Yu, Chen, Yuanbo, Fang, Fei, Zhao, Qun, Zhang, Lihua, Zhang, Yukui. Aptamer-immobilized open tubular capillary column to capture circulating tumor cells for proteome analysis. TALANTA[J]. 2017, 第 7 作者175: 189-193, http://dx.doi.org/10.1016/j.talanta.2017.07.041.
[30] Kaiguang Yang, Jianhui Liu, Jingdi Sun, Yuan Zhou, Qun Zhao, Senwu Li, Lukuan Liu, Lihua Zhang, Jiuyang Zhao, Yukui Zhang. Proteomic study provides new clues for complications of hemodialysis caused by dialysis membrane. Science Bulletin[J]. 2017, 第 5 作者62(18): 1251-1255, https://www.sciengine.com/doi/10.1016/j.scib.2017.08.026.
[31] Zhao, Qun, Fang, Fei, Shan, Yichu, Sui, Zhigang, Zhao, Baofeng, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. In-Depth Proteome Coverage by Improving Efficiency for Membrane Proteome Analysis. ANALYTICAL CHEMISTRY[J]. 2017, 第 1 作者89(10): 5179-5185, http://dx.doi.org/10.1021/acs.analchem.6b04232.
[32] Sui, Zhigang, Weng, Yejing, Zhao, Qun, Deng, Nan, Fang, Fei, Zhu, Xudong, Shan, Yichu, Zhang, Lihua, Zhang, Yukui. Ionic liquid-based method for direct proteome characterization of velvet antler cartilage. TALANTA[J]. 2016, 第 3 作者161: 541-546, http://cas-ir.dicp.ac.cn/handle/321008/169798.
[33] Fang, Fei, Zhao, Qun, Li, Xiao, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. Dissolving capability difference based sequential extraction: A versatile tool for in-depth membrane proteome analysis. ANALYTICA CHIMICA ACTA[J]. 2016, 第 2 作者945: 39-46, http://cas-ir.dicp.ac.cn/handle/321008/169756.
[34] Zhao, Qun, Fang, Fei, Wu, Ci, Wu, Qi, Liang, Yu, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. imFASP: An integrated approach combining in-situ filter-aided sample pretreatment with microwave-assisted protein digestion for fast and efficient proteome sample preparation. ANALYTICA CHIMICA ACTA[J]. 2016, 第 1 作者912: 58-64, http://dx.doi.org/10.1016/j.aca.2016.01.049.
[35] Fang, Fei, Zhao, Qun, Sui, Zhigang, Liang, Yu, Jiang, Hao, Yang, Kaiguang, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. Glycan Moieties as Bait to Fish Plasma Membrane Proteins. ANALYTICAL CHEMISTRY[J]. 2016, 第 2 作者88(10): 5065-5071, http://dx.doi.org/10.1021/acs.analchem.6b01082.
[36] Zhang, Shen, Wu, Qi, Shan, Yichu, Zhao, Qun, Zhao, Baofeng, Weng, Yejing, Sui, Zhigang, Zhang, Lihua, Zhang, Yukui. Fast MS/MS acquisition without dynamic exclusion enables precise and accurate quantification of proteome by MS/MS fragment intensity. SCIENTIFIC REPORTS[J]. 2016, 第 4 作者6: http://dx.doi.org/10.1038/srep26392.
[37] Liang, Yu, Wu, Ci, Zhao, Qun, Wu, Qi, Jiang, Bo, Weng, Yejing, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. Gold nanoparticles immobilized hydrophilic monoliths with variable functional modification for highly selective enrichment and on-line deglycosylation of glycopeptides. ANALYTICA CHIMICA ACTA[J]. 2015, 第 3 作者900: 83-89, http://dx.doi.org/10.1016/j.aca.2015.10.024.
[38] Dong, Yan, Zhao, Qun, Ma, Xiaoyan, Ma, Guowu, Liu, Caiyun, Chen, Zhuwen, Yu, Liyuan, Liu, Xuefeng, Zhang, Yanguang, Shao, Shujuan, Xiao, Jing, Li, Jia, Zhang, Weimin, Fu, Ming, Dong, Lijia, Yang, Xiandong, Guo, Xu, Xue, Liyan, Fang, Fei, Zhan, Qimin, Zhang, Lihua. Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach. SCIENTIFIC REPORTS[J]. 2015, 第 2 作者5(1): http://dx.doi.org/10.1038/srep12668.
[39] 赵群, 方菲, 张丽华, 张玉奎. MS friendly smple preparation techniques for proteome analysis. the 2014 annual conference of the korean society for mass spectrometry. 2014, 第 1 作者http://cas-ir.dicp.ac.cn/handle/321008/143631.
[40] Yang, Kaiguang, Liu, Jianxi, Li, Senwu, Li, Qinran, Wu, Qi, Zhou, Yuan, Zhao, Qun, Deng, Nan, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. Epitope imprinted polyethersulfone beads by self-assembly for target protein capture from the plasma proteome. CHEMICAL COMMUNICATIONS[J]. 2014, 第 7 作者50(67): 9521-9524, http://dx.doi.org/10.1039/c4cc03428g.
[41] 赵群, 方菲, 梁振, 张丽华, 张玉奎. Hela 细胞蛋白质表达谱的深度解析. The 5th Asia Oceania Mass Spectrometry Conference & The 33rd Chinese Mass Spectrometry Society Annual Conference. 2014, 第 1 作者http://cas-ir.dicp.ac.cn/handle/321008/143637.
[42] Zhao, Qun, Fang, Fei, Liang, Yu, Yuan, Huiming, Yang, Kaiguang, Wu, Qi, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. 1-Dodecyl-3-Methylimidazolium Chloride-Assisted Sample Preparation Method for Efficient Integral Membrane Proteome Analysis. ANALYTICAL CHEMISTRY[J]. 2014, 第 1 作者86(15): 7544-7550, http://cas-ir.dicp.ac.cn/handle/321008/144459.
[43] Qiao, Xiaoqiang, Wang, Rui, Yan, Hongyuan, Wang, Tao, Zhao, Qun, Zhang, Lihua, Zhang, Yukui. Development of a novel imidazolium-based aromatic quaternary ammonium tag: Synthesis and application to the efficient analysis of cysteinyl-peptides by mass spectrometry. RAPID COMMUNICATIONS IN MASS SPECTROMETRY[J]. 2014, 第 5 作者28(3): 256-264, http://cas-ir.dicp.ac.cn/handle/321008/144440.
[44] 赵群, 方菲, 随志刚, 张丽华, 张玉奎. BIPHASIC MICROREACTOR FOR EFFICIENT MEMBRANE PROTEIN ANALYSIS OF RARE CELL SAMPLE. the 15th beijing conference and exhibition on instrumental analysis (bceia2013). 2013, 第 1 作者1147, http://159.226.238.44/handle/321008/120117.
[45] Zhao, Qun, Liang, Yu, Yuan, Huiming, Sui, Zhigang, Wu, Qi, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. Biphasic Microreactor for Efficient Membrane Protein Pretreatment with a Combination of Formic Acid Assisted Solubilization, On-Column pH Adjustment, Reduction, Alkylation, and Tryptic Digestion. ANALYTICAL CHEMISTRY[J]. 2013, 第 1 作者85(18): 8507-8512, http://dx.doi.org/10.1021/ac402076u.
[46] 吴慈, 梁玉, 赵群, 曲焱焱, 梁振, 张丽华, 张玉奎. Gold nanoparticles modified hydrophilic polymer monoliths functionalized with boronate acid for specific capture of glycoproteins. 22nd international symposium on glycoconjugates. 2013, 第 3 作者366, http://159.226.238.44/handle/321008/120129.
[47] 赵群, 方菲, 张丽华, 张玉奎. NEW METHODS FOR MEMBRANE PROTEOME ANANLYSIS. the 15th beijing conference and exhibition on instrumental analysis (bceia2013). 2013, 第 1 作者1356, http://159.226.238.44/handle/321008/120122.
[48] Sui, Zhigang, Yuan, Huiming, Liang, Zhen, Zhao, Qun, Wu, Qi, Xia, Simin, Zhang, Lihua, Huo, Yushu, Zhang, Yukui. An activity-maintaining sequential protein extraction method for bioactive assay and proteome analysis of velvet antlers. TALANTA[J]. 2013, 第 4 作者107: 189-194, http://dx.doi.org/10.1016/j.talanta.2013.01.015.
[49] 赵群, 张丽华, 张玉奎. Novel Sample Preparation Strategy for High Efficient Integral Membrane Protein Analysis. 39th international symposium on high performance liquid phase separations and related techniques. 2013, 第 1 作者177, http://159.226.238.44/handle/321008/120225.
[50] 赵群, 方菲, 张丽华, 张玉奎. Novel Sample Preparation Materials for Proteome Analysis. the 15th beijing conference and exhibition on instrumental analysis. 2013, 第 1 作者1354, http://159.226.238.44/handle/321008/120121.
[51] 随志刚, 赵群, 邓楠, 蒋好, 梁振, 张丽华, 张玉奎. A new strategy for studying proteome of velvet antler cartilage. 40th international symposium on high performance liquid phase separations and related techiniques 2013. 2013, 第 2 作者134, http://159.226.238.44/handle/321008/120124.
[52] 随志刚, 赵群, 吴琪, 夏思敏, 张丽华, 霍玉书, 张玉奎. Development of Sequential Protein Extraction Method for Bioactive and Proteome Analysis of Velvet Antlers. asia oceania human proteome organization 6th congress. 2012, 第 2 作者284, http://159.226.238.44/handle/321008/117090.
[53] Zhao, Qun, Sun, Liangliang, Liang, Yu, Wu, Qi, Yuan, Huiming, Liang, Zhen, Zhang, Lihua, Zhan, Yukui. Prefractionation and separation by C8 stationary phase: Effective strategies for integral membrane proteins analysis. TALANTA[J]. 2012, 第 1 作者88: 567-572, http://dx.doi.org/10.1016/j.talanta.2011.11.035.
[54] 赵群, 袁辉明, 吴琪, 梁振, 张丽华, 张玉奎. Mechanism Study of Lonic Liquids for Solubilizing Membrane Proteins. asia oceania human proteome organization 6th congress. 2012, 第 1 作者306, http://159.226.238.44/handle/321008/117091.
[55] Wu, Qi, Zhao, Qun, Liang, Zhen, Qu, Yanyan, Zhang, Lihua, Zhang, Yukui. NSI and NSMT: usages of MS/MS fragment ion intensity for sensitive differential proteome detection and accurate protein fold change calculation in relative label-free proteome quantification. ANALYST[J]. 2012, 第 2 作者137(13): 3146-3153, http://159.226.238.44/handle/321008/143105.
[56] Jiang, Bo, Yang, Kaiguang, Zhao, Qun, Wu, Qi, Liang, Zhen, Zhang, Lihua, Peng, Xiaojun, Zhang, Yukui. Hydrophilic immobilized trypsin reactor with magnetic graphene oxide as support for high efficient proteome digestion. JOURNAL OF CHROMATOGRAPHY A[J]. 2012, 第 3 作者1254: 8-13, http://dx.doi.org/10.1016/j.chroma.2012.07.030.
[57] Sun, Liangliang, Zhang, Yanyan, Tao, Dingyin, Zhu, Guijie, Zhao, Qun, Wu, Qi, Liang, Zhen, Yang, Ling, Zhang, Lihua, Zhang, Yukui. SDS-PAGE-free protocol for comprehensive identification of cytochrome P450 enzymes and uridine diphosphoglucuronosyl transferases in human liver microsomes. PROTEOMICS[J]. 2012, 第 5 作者12(23-24): 3464-3469, https://www.doi.org/10.1002/pmic.201200260.
[58] Jiang, Hao, Yuan, Huiming, Liang, Yu, Xia, Simin, Zhao, Qun, Wu, Qi, Zhang, Lihua, Liang, Zhen, Zhang, Yukui. A hydrophilic immobilized trypsin reactor with N-vinyl-2-pyrrolidinone modified polymer microparticles as matrix for highly efficient protein digestion with low peptide residue. JOURNAL OF CHROMATOGRAPHY A[J]. 2012, 第 5 作者1246: 111-116, http://dx.doi.org/10.1016/j.chroma.2012.03.014.
[59] 赵群, 孙良亮, 袁辉明, 吴琪, 梁振, 张丽华, 张玉奎. An efficient method for membrane protein prefractionation based on the difference of the solvent solubility. 37th international symposium on high-performance liquid phase separations and related techniques. 2011, 第 1 作者462, http://159.226.238.44/handle/321008/117405.
[60] 蒋好, 袁辉明, 夏思敏, 赵群, 吴琪, 张丽华, 张玉奎. A Hydrophilic Immobilized Trypsin Reactor In Capillary For Highly Efficient Protein Digestion. 37th international symposium on high-performance liquid phase separations and related techniques. 2011, 第 4 作者478, http://159.226.238.44/handle/321008/117401.
[61] 赵群, 孙良亮, 吴琪, 袁辉明, 梁振, 张丽华, 张玉奎. Analysis of integral membrane proteome by micro HPLC coupled with MALDI-TOF-MS. 7th cnupo annual congress and 3rd international forum of proteomics proteomics and translational medicine. 2011, 第 1 作者132, http://159.226.238.44/handle/321008/117398.
[62] Sun, Liangliang, Zhao, Qun, Zhu, Guijie, Zhou, Yuan, Wang, Tingting, Shan, Yichu, Yang, Kaiguang, Liang, Zhen, Zhang, Lihua, Zhang, Yukui. Octyl-functionalized hybrid magnetic mesoporous microspheres for enrichment of low-concentration peptides prior to direct analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. RAPID COMMUNICATIONS IN MASS SPECTROMETRY[J]. 2011, 第 2 作者25(9): 1257-1265, http://www.irgrid.ac.cn/handle/1471x/456671.
[63] 梁玉, 赵群, 吴慈, 梁振, 张丽华, 张玉奎. Hydrophilic Monolith Based Frits for Preparation of lin-exchange Packed Capillary Column. 37th international symposium on high-performance liquid phase separations and related techniques. 2011, 第 2 作者274, http://159.226.238.44/handle/321008/117410.
[64] 随志刚, 袁辉明, 赵群, 吴琪, 高亮, 梁振, 张丽华, 霍玉书, 张玉奎. A sequential protein extraction strategy for analysis of deer antler proteome and biological activity. 37th international symposium on high-performance liquid phase separations and related techniques. 2011, 第 3 作者463, http://159.226.238.44/handle/321008/117404.
[65] 赵群, 孙良亮, 吴琪, 梁振, 张丽华, 张玉奎. Effect of alkyl chain length on reversed phase stationary phase for separation of integral membrane protein digests. 26th international symposium on microscale bioseparations. 2011, 第 1 作者75, http://159.226.238.44/handle/321008/117389.
[66] 孙良亮, 陶定银, 赵群, 张玉奎. Ionic Liquids Based Solvent System for Rat brain membrane proteome analysis. 全球第九届人类蛋白质组学会议. 2010, 第 3 作者121/2, http://159.226.238.44/handle/321008/114162.

   

（ 1 ） 基于新型样品预处理和质谱全离子监测的蛋白组深度覆盖定量方法研究, 负责人, 国家任务, 2016-01--2018-12
（ 2 ） 蛋白质复合物规模化分析鉴定新方法研究, 参与, 国家任务, 2016-07--2021-07
（ 3 ） 蛋白质机器的动态结构的原位捕获和分析方法, 负责人, 国家任务, 2018-05--2023-04
（ 4 ） 基于新型化学交联剂的信号转导全通路蛋白质复合物的精准解析, 负责人, 研究所自选, 2018-01--2020-12
（ 5 ） 基于糖苷键质谱可碎裂型化学交联剂的研制及其在蛋白质复合物解析中的应用, 负责人, 国家任务, 2018-01--2021-12

（1）Ionic Liquid Based Sample Preparation Strategy for Efficient Proteome Analysis   2016-08-08
（2）Ionic liquid-based filter-aided sample preparation for in-depth proteome analysis   2016-06-03
（3）An Integrated Approach to In-depth Profile of A Human Cancer Cell Line   2015-04-26