基本信息
张佳 女 硕导 中国科学院青岛生物能源与过程研究所
电子邮件: zhangjia@qibebt.ac.cn
通信地址: 山东青岛崂山区松岭路189号
邮政编码:
电子邮件: zhangjia@qibebt.ac.cn
通信地址: 山东青岛崂山区松岭路189号
邮政编码:
研究领域
1. 基因工程平台搭建:研发基因合成技术(包括基于芯片的DNA头合成技术、基于酶法的DNA从头合成、基因的拼装、基因从头合成的设计、DNA合成纠错系统构建等),构建高通量、高保真基因的“写”,进而搭建富有显著技术特色与业界竞争力的基因工程平台。
2. 代谢工程:基于基因工程平台改造酵母等代谢途径,使其高温高产木糖醇和乙醇等研究,获得可产业化的工程菌株,将木糖醇的生产由化学法转变成生物法。
3. 酶工程:基于基因工程平台进行酶工程改造,提高酶的稳定性及其活性等,实现相关酶试剂盒的研发及其产业化,从工具酶自主研发角度构建单细胞拉曼组核心竞争力。已研发的试剂盒包括:单细胞全基因组扩增试剂盒、Tn5建库试剂盒、逆转录试剂盒、DNA合成纠错系统等。
4. 单细胞拉曼组技术及其应用示范:从工具酶自主创新的角度构建单细胞拉曼技术的核心竞争力,促进基于拉曼组的相关生物学研究,开展和服务单细胞精度的生命系统和生物过程研究及其应用示范(茅台集团、中粮集团、伊利集团等)。
招生信息
招生专业
071010-生物化学与分子生物学071005-微生物学0710J5-食品安全与健康
招生方向
蛋白质工程,酶工程基因合成代谢工程
教育背景
2010-09--2015-06 中国科学技术大学 博士
工作经历
工作简历
2023-01~现在, 中国科学院青岛生物能源与过程研究所, 副研究员2017-08~2022-12,中国科学院青岛生物能源与过程研究所, 助理研究员2016-12~2017-03,休斯顿大学, 访问学者2015-07~2017-08,中国科学院青岛生物能源与过程研究所, 博士后
社会兼职
2025-06-23-今,mSystems编辑,
2023-12-20-2028-12-31,BioDesign Research首届青年编委,
2023-12-20-2028-12-31,BioDesign Research首届青年编委,
出版信息
发表论文
(1) Label-free, single-cell-precise, and monomeric-unit-resolved monitoring of biopolymer fermentation by ramanomics, Trends in Biotechnology, 2026, 第 1 作者(2) Engineered eMutS based magnetic separation system for high-fidelity DNA synthesis, Synthetic and Systems Biotechnology, 2026, 第 11 作者 通讯作者(3) Unlocking Microbial Dark Matter: A Comprehensive Review of Isolation Technologies from Traditional Culturing to Single-Cell Technologies, Microorganisms, 2026, 第 3 作者 通讯作者(4) Phylogeny-metabolism dual-directed single-cell genomics for dissecting and mining ecosystem function by FISH-scRACS-seq, Phylogeny-metabolism dual-directed single-cell genomics for dissecting and mining ecosystem function by FISH-scRACS-seq, THE INNOVATION, 2025, 第 11 作者(5) Raman flow cytometry based single‐cell species classification, viable‐cell counting and vitality test for probiotic products, IMETAOMICS, 2025, 第 1 作者 通讯作者(6) Assessing the physiological properties of baker's yeast based on single-cell Raman spectrum technology, SYNTHETIC AND SYSTEMS BIOTECHNOLOGY, 2024, 第 12 作者 通讯作者(7) Improved single-cell genome amplification by a high-efficiency phi29 DNA polymerase, Frontiers in Bioengineering and Biotechnology, 2023, 第 1 作者(8) Single‐cell rapid identification, in situ viability and vitality profiling, and genome‐based source‐tracking for probiotics products, iMeta, 2023, 第 1 作者(9) Culture-free identification of fast-growing cyanobacteria cells by Raman-activated gravity-driven encapsulation and sequencing, Culture-free identification of fast-growing cyanobacteria cells by Raman-activated gravity-driven encapsulation and sequencing, SYNTHETIC AND SYSTEMS BIOTECHNOLOGY, 2023, 第 8 作者(10) Lignocellulosic xylitol production from corncob waste by engineered Kluyveromyces marxianus, Frontiers in Bioengineering and Biotechnology, 2022, 第 1 作者(11) Efficient and Low-Cost Error Removal in DNA Synthesis by a High-Durability MutS, ACS SYNTHETIC BIOLOGY, 2020, 第 1 作者(12) Seasonal dynamics of the coastal bacterioplankton at intensive fish-farming areas of the Yellow Sea, China revealed by high-throughput sequencing, MARINE POLLUTION BULLETIN, 2019, 第 6 作者(13) Engineered Kluyveromyces marxianus for pyruvate production at elevated temperature with simultaneous consumption of xylose and glucose, BIORESOURCETECHNOLOGY, 2017, 第 3 作者(14) Simultaneous fermentation of glucose and xylose at elevated temperatures co-produces ethanol and xylitol through overexpression of a xylose-specific transporter in engineered Kluyveromyces marxianus, BIORESOURCE TECHNOLOGY, 2016, 第 2 作者(15) Improving xylitol production at elevated temperature with engineered Kluyveromyces marxianus through over-expressing transporters, BIORESOURCE TECHNOLOGY, 2015, 第 1 作者(16) Rapid ethanol production at elevated temperatures by engineered thermotolerant Kluyveromyces marxianus via the NADP(H)-preferring xylose reductase-xylitol dehydrogenase pathway, METABOLIC ENGINEERING, 2015, 第 1 作者(17) Xylitol production at high temperature by engineered Kluyveromyces marxianus, BIORESOURCE TECHNOLOGY, 2014, 第 1 作者(18) Error removal in microchip-synthesized DNA using immobilized MutS, NUCLEIC ACIDS RESEARCH, 2014, 第 7 作者