微生物的基因编辑育种与一碳原料的生物转化利用。针对工业微生物，开发了基于CRISPR的高效基因组编辑、多基因精细表达调控等先进的合成生物学使能技术，设计创制了氨基酸等生物基化学品的工业微生物菌种，拓展了工业微生物的底物谱，实现了甲醇等一碳原料的高效生物转化利用，为解决我国生物制造工业菌种的“卡脖子”问题提供源头创新和技术支撑。

   

085238-生物工程

合成生物学，代谢工程

2012-09--2016-03   上海交通大学   博士
2009-09--2012-06   山东大学   硕士
2005-09--2009-07   山东大学   本科

   

2022-06~现在, 中国科学院天津工业生物技术研究所, 研究员
2018-12~2022-06,中国科学院天津工业生物技术研究所, 副研究员
2016-04~2018-12,中国科学院天津工业生物技术研究所, 助理研究员

   

（1） DaSilva Award, 其他, 2022
（2） 《麻省理工科技评论》亚太区“35岁以下科技创新35人”, 其他, 2022
（3） “伦世仪教育基金”杰出青年学者, 其他, 2022
（4） 天津市优秀科技志愿者, 省级, 2020
（5） 上海交通大学优秀博士学位论文提名论文, 研究所（学校）, 2016
（6） 博士研究生国家奖学金, 国家级, 2015
（7） 上海市明治生命科学奖, 市地级, 2014
（8） 博士研究生国家奖学金, 国家级, 2014
（9） 挑战杯中国大学生创业计划竞赛金奖, 国家级, 2010

（ 1 ） 具有天冬氨酸激酶活性的多肽及其在生产氨基酸中的应用, 发明专利, 2021, 第 3 作者, 专利号: CN113201514A

（ 2 ） 一种提高微生物对甲醇的耐受性和利用率的方法, 发明专利, 2021, 第 2 作者, 专利号: CN112680389A

（ 3 ） 一种提高菌株的甲醇生物耐受性和生物转化效率的方法, 发明专利, 2021, 第 2 作者, 专利号: CN112680468A

（ 4 ） γ-谷氨酰激酶突变体及其应用, 专利授权, 2021, 第 3 作者, 专利号: CN112111469B

（ 5 ） 赖氨酸生产菌株的构建方法和应用, 专利授权, 2021, 第 7 作者, 专利号: CN110129247B

（ 6 ） 一种赖氨酸生产菌株的构建方法和应用, 发明专利, 2021, 第 7 作者, 专利号: CN112251391A

（ 7 ） 5-氨基乙酰丙酸生产菌株及其构建方法和应用, 发明专利, 2020, 第 5 作者, 专利号: CN110862952A

（ 8 ） 甲醇生物转化菌株的构建方法以及构建的菌株和应用, 发明专利, 2020, 第 2 作者, 专利号: CN110760536A

（ 9 ） 一种菌及其构建方法和应用, 发明专利, 2016, 第 2 作者, 专利号: CN106190936A

（ 10 ） 一种菌及其获取方法和应用, 发明专利, 2016, 第 4 作者, 专利号: CN106190901A

（ 11 ） 一种利用基因工程蓝藻生产三碳化合物的方法, 发明专利, 2015, 第 2 作者, 专利号: CN104726505A

   

[1] Pu, Wei, Chen, Jiuzhou, Zhou, Yingyu, Qiu, Huamin, Shi, Tuo, Zhou, Wenjuan, Guo, Xuan, Cai, Ningyun, Tan, Zijian, Liu, Jiao, Feng, Jinhui, Wang, Yu, Zheng, Ping, Sun, Jibin. Systems metabolic engineering of Escherichia coli for hyper-production of 5-aminolevulinic acid. BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS[J]. 2023, 第 12 作者  通讯作者  16(1): http://dx.doi.org/10.1186/s13068-023-02280-9.
[2] Pu, Wei, Chen, Jiuzhou, Liu, Pi, Shen, Jie, Cai, Ningyun, Liu, Baoyan, Lei, Yu, Wang, Lixian, Ni, Xiaomeng, Zhang, Jie, Liu, Jiao, Zhou, Yingyu, Zhou, Wenjuan, Ma, Hongwu, Wang, Yu, Zheng, Ping, Sun, Jibin. Directed evolution of linker helix as an efficient strategy for engineering LysR-type transcriptional regulators as whole-cell biosensors. BIOSENSORS & BIOELECTRONICS[J]. 2023, 第 15 作者  通讯作者  222: http://dx.doi.org/10.1016/j.bios.2022.115004.
[3] Bo Xin, Cheng Zhong, Yu Wang. Integrating the marine carbon resource mannitol into biomanufacturing. TRENDS IN BIOTECHNOLOGY[J]. 2023, 第 3 作者  通讯作者  41(6): 745-749, http://dx.doi.org/10.1016/j.tibtech.2022.12.010.
[4] 刘叶, 刘扬, 郑平, 王钰, 王猛. Cytosine Base Editing in Bacteria. Methods in Molecular Biology[J]. 2023, 第 4 作者https://doi.org/10.1007/978-1-0716-2879-9_17,.
[5] 王钰, 武陶, 樊旭倩, 阮海华, 樊飞宇, 张学礼, , , , , , . 酵母甾醇转运蛋白研究进展. 生物工程学报[J]. 2023, 第 1 作者39(8): 3204-3218, https://cjb.ijournals.cn/cjbcn/article/abstract/gc23083204?st=article_issue.
[6] 蒲伟, 陈久洲, 王钰, 郑平, 孙际宾, , , , , . 氨基酸生物传感器的开发及应用研究进展. 生物工程学报[J]. 2023, 第 3 作者39(6): 2485-2501, https://cjb.ijournals.cn/cjbcn/article/abstract/gc23062485?st=article_issue.
[7] 卢挥, 张启, 于思礼, 王钰, 康明, 韩双艳, 刘叶, 王猛, , , , , , , , . 谷氨酸棒杆菌中基于CRISPR/Cas9的多位点碱基编辑系统的优化. 生物工程学报[J]. 2022, 第 4 作者38(2): 780-795, https://cjb.ijournals.cn/cjbcn/article/abstract/gc22020780?st=article_issue.
[8] 蔡韬, 刘玉万, 朱蕾蕾, 苏浩, 王钰, 王国坤, 张玲玲, 朱之光, 盛翔, 毕昌昊, 马红武, 田朝光, 张学礼, 吴洽庆, 孙媛霞, 江会锋, 马延和, , , , , , , , , , , , , , , , , . 二氧化碳人工生物转化. 生物工程学报[J]. 2022, 第 5 作者38(11): 4101-4114, https://cjb.ijournals.cn/cjbcn/article/abstract/gc22114101?st=article_issue.
[9] 周文娟, 付刚, 齐显尼, 郑小梅, 房欢, 夏苗苗, 张大伟, 王钦宏, 郑平, 王钰, 孙际宾, , , , , , , , , , , . 发酵工业菌种的迭代创制. 生物工程学报[J]. 2022, 第 10 作者38(11): 4200-4218, https://cjb.ijournals.cn/cjbcn/article/abstract/gc22114200?st=article_issue.
[10] Jiuzhou Chen, Yu Wang, Ping Zheng, jibin sun. Engineering synthetic auxotrophs for growth-coupled directed protein evolution. Trends in Biotechnology[J]. 2022, 第 2 作者  通讯作者  
[11] Jiao Liu, Moshi Liu, Tuo Shi, Guannan Sun, Ning Gao, Xiaojia Zhao, Xuan Guo, Xiaomeng Ni, Qianqian Yuan, Jinhui Feng, Zhemin Liu, Yanmei Guo, Jiuzhou Chen, Yu Wang, Ping Zheng, Jibin Sun. CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an l -proline exporter for l -proline hyperproduction. NATURE COMMUNICATIONS[J]. 2022, 第 14 作者  通讯作者  13: 
[12] Wang, Yu, Zhao, Dongdong, Sun, Letian, Wang, Jie, Fan, Liwen, Cheng, Guimin, Zhang, Zhihui, Ni, Xiaomeng, Feng, Jinhui, Wang, Meng, Zheng, Ping, Bi, Changhao, Zhang, Xueli, Sun, Jibin. Engineering of the Translesion DNA Synthesis Pathway Enables Controllable C-to-G and C-to-A Base Editing in Corynebacterium glutamicum. ACS SYNTHETIC BIOLOGY[J]. 2022, 第 1 作者11(10): 3368-3378, http://dx.doi.org/10.1021/acssynbio.2c00265.
[13] Wei Pu, Jiuzhou Chen, Liu, Pi, Jie Shen, Ningyun Cai, Baoyan Liu, Yu Lei, Lixian Wang, Xiaomeng Ni, Jie Zhang, Jiao Liu, Yingyu Zhou, Wenjuan Zhou, Hongwu Ma, Yu Wang, ping zheng, Jibin Sun. Directed evolution of linker helix as an efficient strategy for engineering LysR-type transcriptional regulators as whole-cell biosensors. Biosensors and Bioelectronics[J]. 2022, 第 15 作者  通讯作者  222: 
[14] Sun, Xue, Li, Qinggang, Wang, Yu, Zhou, Wenjuan, Guo, Yanmei, Chen, Jiuzhou, Zheng, Ping, Sun, Jibin, Ma, Yanhe. Isoleucyl-tRNA synthetase mutant based whole-cell biosensor for high-throughput selection of isoleucine overproducers. BIOSENSORS & BIOELECTRONICS[J]. 2021, 第 3 作者172: http://dx.doi.org/10.1016/j.bios.2020.112783.
[15] 凡立稳, 王钰, 郑平, 孙际宾, , , , . 一碳代谢关键酶——甲醇脱氢酶的研究进展与展望. 生物工程学报[J]. 2021, 第 2 作者37(2): 530-540, https://cjb.ijournals.cn/cjbcn/article/abstract/gc21020530?st=article_issue.
[16] Yu Wang, Haijiao Cheng, Yang Liu, Ye Liu, Xiao Wen, Kun Zhang, Xiaomeng Ni, Ning Gao, Liwen Fan, Zhihui Zhang, Jiao Liu, Jiuzhou Chen, Lixian Wang, Yanmei Guo, Ping Zheng, Meng Wang, Jibin Sun, Yanhe Ma. In-situ generation of large numbers of genetic combinations for metabolic reprogramming via CRISPR-guided base editing. NATURE COMMUNICATIONS[J]. 2021, 第 1 作者  通讯作者  12(1): http://dx.doi.org/10.1038/s41467-021-21003-y.
[17] Zhang, Zhihui, Wang, Yu, Zheng, Ping, Sun, Jibin. Promoting Lignin Valorization by Coping with Toxic C1 Byproducts. TRENDS IN BIOTECHNOLOGY. 2021, 第 2 作者  通讯作者  39(4): 331-335, http://dx.doi.org/10.1016/j.tibtech.2020.09.005.
[18] 陈久洲, 王钰, 蒲伟, 郑平, 孙际宾. 5-氨基乙酰丙酸生物合成技术的发展及展望. 合成生物学[J]. 2021, 第 2 作者2(6): 1000-1016, http://lib.cqvip.com/Qikan/Article/Detail?id=7106434784.
[19] Wang, Yu, Liu, Ye, Zheng, Ping, Sun, Jibin, Wang, Meng. Microbial Base Editing: A Powerful Emerging Technology for Microbial Genome Engineering. TRENDS IN BIOTECHNOLOGY. 2021, 第 1 作者  通讯作者  39(2): 165-180, http://dx.doi.org/10.1016/j.tibtech.2020.06.010.
[20] Li, Mingyue, Chen, Jiuzhou, Wang, Yu, Liu, Jiao, Huang, Jingwen, Chen, Ning, Zheng, Ping, Sun, Jibin. Efficient Multiplex Gene Repression by CRISPR-dCpf1 in Corynebacterium glutamicum. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY[J]. 2020, 第 3 作者8: https://doaj.org/article/f87f64c6d0d9420fbbd0fbdea99eda61.
[21] Yu, Sili, Price, Marcus A, Wang, Yu, Liu, Yang, Guo, Yanmei, Ni, Xiaomeng, Rosser, Susan J, Bi, Changhao, Wang, Meng. CRISPR-dCas9 Mediated Cytosine Deaminase Base Editing in Bacillus subtilis. ACS SYNTHETIC BIOLOGY[J]. 2020, 第 3 作者9(7): 1781-1789, https://www.webofscience.com/wos/woscc/full-record/WOS:000551555500027.
[22] Wang, Yu, Fan, Liwen, Tuyishime, Philibert, Liu, Jiao, Zhang, Kun, Gao, Ning, Zhang, Zhihui, Ni, Xiaomeng, Feng, Jinhui, Yuan, Qianqian, Ma, Hongwu, Zheng, Ping, Sun, Jibin, Ma, Yanhe. Adaptive laboratory evolution enhances methanol tolerance and conversion in engineered Corynebacterium glutamicum. COMMUNICATIONS BIOLOGY[J]. 2020, 第 1 作者3(1): http://dx.doi.org/10.1038/s42003-020-0954-9.
[23] Chen, Jiuzhou, Wang, Yu, Guo, Xuan, Rao, Deming, Zhou, Wenjuan, Zheng, Ping, Sun, Jibin, Ma, Yanhe. Efficient bioproduction of 5-aminolevulinic acid, a promising biostimulant and nutrient, from renewable bioresources by engineered Corynebacterium glutamicum. BIOTECHNOLOGY FOR BIOFUELS[J]. 2020, 第 2 作者13(1): https://doaj.org/article/76327970ce5a4277b911a00b0b226b6a.
[24] Wang, Yu, Fan, Liwen, Tuyishirne, Philibert, Zheng, Ping, Sun, Jibin. Synthetic Methylotrophy: A Practical Solution for Methanol-Based Biomanufacturing. TRENDS IN BIOTECHNOLOGY[J]. 2020, 第 1 作者38(6): 650-666, http://dx.doi.org/10.1016/j.tibtech.2019.12.013.
[25] 李俊维, 刘叶, 王钰, 郁彭, 郑平, 王猛, , , , , , . 谷氨酸棒杆菌碱基编辑的条件优化. 生物工程学报[J]. 2020, 第 3 作者36(1): 143-151, https://cjb.ijournals.cn/cjbcn/article/abstract/gc20010143?st=article_issue.
[26] Zhang, Kun, Zhang, Zhihui, Kang, Jianan, Chen, Jiuzhou, Liu, Jiao, Gao, Ning, Fan, Liwen, Zheng, Ping, Wang, Yu, Sun, Jibin. CRISPR/Cas13d-Mediated Microbial RNA Knockdown. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY[J]. 2020, 第 9 作者  通讯作者  8: https://doaj.org/article/d85c16031ef74534b0f4a19a090235cc.
[27] Sun, Dehu, Chen, Jiuzhou, Wang, Yu, Li, Mingyue, Rao, Deming, Guo, Yanmei, Chen, Ning, Zheng, Ping, Sun, Jibin, Ma, Yanhe. Metabolic engineering of Corynebacterium glutamicum by synthetic small regulatory RNAs. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY[J]. 2019, 第 3 作者46(2): 203-208, https://www.webofscience.com/wos/woscc/full-record/WOS:000457989900007.
[28] Wang, Yu, Liu, Ye, Li, Junwei, Yang, Yi, Ni, Xiaomeng, Cheng, Haijiao, Huang, Teng, Guo, Yanmei, Ma, Hongwu, Zheng, Ping, Wang, Meng, Sun, Jibin, Ma, Yanhe. Expanding targeting scope, editing window, and base transition capability of base editing in Corynebacterium glutamicum. BIOTECHNOLOGY AND BIOENGINEERING[J]. 2019, 第 1 作者  通讯作者  116(11): 3016-3029, http://dx.doi.org/10.1002/bit.27121.
[29] Zhu, Chengchao, Chen, Jiuzhou, Wang, Yu, Wang, Lixian, Guo, Xuan, Chen, Ning, Zheng, Ping, Sun, Jibin, Ma, Yanhe. Enhancing 5-aminolevulinic acid tolerance and production by engineering the antioxidant defense system of Escherichia coli. BIOTECHNOLOGY AND BIOENGINEERING[J]. 2019, 第 3 作者116(8): 2018-2028, 
[30] Fan, Liwen, Wang, Yu, Tuyishime, Philibert, Gao, Ning, Li, Qinggang, Zheng, Ping, Sun, Jibin, Ma, Yanhe. Engineering Artificial Fusion Proteins for Enhanced Methanol Bioconversion. CHEMBIOCHEM[J]. 2018, 第 2 作者19(23): 2465-2471, https://www.webofscience.com/wos/woscc/full-record/WOS:000452035700008.
[31] Wang, Yu, Cao, Guoqiang, Xu, Deyu, Fan, Liwen, Wu, Xinyang, Ni, Xiaomeng, Zhao, Shuxin, Zheng, Ping, Sun, Jibin, Ma, Yanhe. A Novel Corynebacterium glutamicum L-Glutamate Exporter. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2018, 第 1 作者84(6): https://www.webofscience.com/wos/woscc/full-record/WOS:000426389400027.
[32] Liu, Jiao, Wang, Yu, Zheng, Ping, Sun, Jibin. CRISPR/Cas9-mediated ssDNA Recombineering in Corynebacterium glutamicum. BIO-PROTOCOL[J]. 2018, 第 2 作者8(19): http://dx.doi.org/10.21769/BioProtoc.3038.
[33] Wang, Yu, Liu, Ye, Liu, Jiao, Guo, Yanmei, Fan, Liwen, Ni, Xiaomeng, Zheng, Xiaomei, Wang, Meng, Zheng, Ping, Sun, Jibin, Ma, Yanhe. MACBETH: Multiplex automated Corynebacterium glutamicum base editing method. METABOLIC ENGINEERING[J]. 2018, 第 1 作者47: 200-210, http://dx.doi.org/10.1016/j.ymben.2018.02.016.
[34] Liu, Jiao, Wang, Yu, Lu, Yujiao, Ni, Xiaomeng, Guo, Xuan, Zhao, Jing, Chen, Jiuzhou, DeleOsibanjo, Taiwo, Zheng, Ping, Sun, Jibin, Ma, Yanhe. Mutations in Peptidoglycan Synthesis Gene ponA Improve Electrotransformation Efficiency of Corynebacterium glutamicum ATCC 13869. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2018, 第 2 作者84(24): https://www.webofscience.com/wos/woscc/full-record/WOS:000451988000019.
[35] Zhang, Qiongqiong, Zheng, Xiaomei, Wang, Yu, Yu, Jiandong, Zhang, Zhidan, DeleOsibanjo, Taiwo, Zheng, Ping, Sun, Jibin, Jia, Shiru, Ma, Yanhe. Comprehensive optimization of the metabolomic methodology for metabolite profiling of Corynebacterium glutamicum. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2018, 第 3 作者102(16): 7113-7121, https://www.webofscience.com/wos/woscc/full-record/WOS:000440109200029.
[36] Tuyishime, Philibert, Wang, Yu, Fan, Liwen, Zhang, Qiongqiong, Li, Qinggang, Zheng, Ping, Sun, Jibin, Ma, Yanhe. Engineering Corynebacterium glutamicum for methanol-dependent growth and glutamate production. METABOLIC ENGINEERING[J]. 2018, 第 2 作者49: 220-231, http://dx.doi.org/10.1016/j.ymben.2018.07.011.
[37] Xiaolu Wang, Yu Wang, Jiao Liu, Qinggang Li, Zhidan Zhang, Ping Zheng, Fuping Lu, Jibin Sun. Biological conversion of methanol by evolved Escherichia coli carrying a linear methanol assimilation pathway. BIORESOURCES AND BIOPROCESSING[J]. 2017, 第 2 作者4(1): 1-6, http://dx.doi.org/10.1186/s40643-017-0172-6.
[38] Wang, Yu, Tao, Fei, Xin, Bo, Liu, Hongyu, Gao, Yanyan, Zhou, NingYi, Xu, Ping. Switch of metabolic status: redirecting metabolic flux for acetoin production from glycerol by activating a silent glycerol catabolism pathway. METABOLIC ENGINEERING[J]. 2017, 第 1 作者39: 90-101, http://dx.doi.org/10.1016/j.ymben.2016.10.020.
[39] Liu, Jiao, Wang, Yu, Lu, Yujiao, Zheng, Ping, Sun, Jibin, Ma, Yanhe. Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum. MICROBIAL CELL FACTORIES[J]. 2017, 第 2 作者16(1): http://dx.doi.org/10.1186/s12934-017-0815-5.
[40] 王晓璐, 王钰, 刘娇, 郑平, 路福平. 利用定向进化提高基因工程大肠杆菌的甲醇利用能力. 生物技术通报[J]. 2017, 第 2 作者33(9): 101-109, https://d.wanfangdata.com.cn/periodical/swjstb201709012.
[41] Wang, Yu, Tao, Fei, Ni, Jun, Li, Chao, Xu, Ping. Production of C3 platform chemicals from CO2 by genetically engineered cyanobacteria. GREEN CHEMISTRY[J]. 2015, 第 1 作者  通讯作者  17(5): 3100-3110, https://www.webofscience.com/wos/woscc/full-record/WOS:000354409500058.
[42] Wang, Yu, Tao, Fei, Tang, Hongzhi, Xu, Ping. Genome Sequence of Clostridium diolis Strain DSM 15410, a Promising Natural Producer of 1,3-Propanediol. MICROBIOLOGY RESOURCE ANNOUNCEMENTS[J]. 2013, 第 1 作者1(4): 
[43] Wang Yu, Tao Fei, Xu Ping. Glycerol Dehydrogenase Plays a Dual Role in Glycerol Metabolism and 2,3-Butanediol Formation in Klebsiella pneumoniae *. THE JOURNAL OF BIOLOGICAL CHEMISTRY. 第 1 作者289(9): 6080-6090, http://dx.doi.org/10.1074/jbc.M113.525535.
[44] Wang Yu, Tao Fei, Li Chao, Li Lixiang, Xu Ping. Genome Sequence of Klebsiella pneumoniae Strain ATCC 25955, an Oxygen-Insensitive Producer of 1,3-Propanediol. GENOME ANNOUNCEMENTS. 第 1 作者1(4): 

   

（ 1 ） 谷氨酸棒杆菌的甲醇利用限制因素研究, 负责人, 国家任务, 2018-01--2020-12
（ 2 ） 二氧化碳的人工生物转化, 参与, 中国科学院计划, 2016-01--2018-12
（ 3 ） 面向天然产物生物合成的人工细胞, 参与, 中国科学院计划, 2018-01--2022-12
（ 4 ） UriR/RbsR参与谷氨酸棒杆菌抵御高温压力的机制研究, 参与, 国家任务, 2019-01--2022-12
（ 5 ） 高版本模式微生物底盘细胞, 参与, 国家任务, 2019-07--2024-06
（ 6 ） 超进化聚球藻底盘细胞的设计构建, 参与, 国家任务, 2019-07--2024-06
（ 7 ） 甲醇为唯一碳源的谷氨酸棒杆菌构建及机制解析, 负责人, 国家任务, 2021-01--2024-12
（ 8 ） 基金委优秀青年基金—微生物基因编辑育种, 负责人, 国家任务, 2023-01--2025-12

（1）Genome Engineering of Corynebacterium glutamicum for C1-based Biomanufacturing   2019-01-13
（2）Learn from the industrial Corynebacterium glutamicum strains   2018年天津科大-天津工业生物所中青年学者学术论坛   2018-11-06
（3）工业平台菌株C1底物谱拓展及应用   2018年中国微生物学会学术年会   2018-10-21
（4）非糖质原料的生物转化与化学品合成   中国生物工程学会第三届青年科技论坛   2018-04-15