1. 糖蛋白糖链的纯化制备和结构分析
以糖基化蛋白质所连接的糖链为对象，通过发展糖链的释放、富集、多维色谱分离与纯化制备、串联质谱分析以及纯度和含量测定等关键方法与技术，获取高纯度的糖链样品并对其结构进行鉴定，构建糖链的标准品库，为糖链生物学功能研究提供标准样品。

2. 糖肽富集与糖蛋白质组学分析
在新型亲水作用色谱分离材料的基础上，发展以糖基化肽段的高选择性富集技术为核心的糖蛋白质组学分析方法，对复杂生物体系（如血清、酵母细胞等）中蛋白质的糖基化情况进行深入解析，为糖蛋白生物学功能的研究奠定结构基础。

3. 蛋白质定量糖基化分析研究
发展糖链的高效液相色谱分离方法和高灵敏度质谱分析方法，在此基础上结合糖链标准品库，建立糖链的绝对定量分析方法，描述癌症状态下血清糖蛋白所发生的异常糖基化改变，为癌症血清标志物的筛查提供理论依据和技术支撑。

   

070302-分析化学

质谱分析
色谱分析
糖生物学

2006-09--2011-03 中国科学院大连化学物理研究所 博士
2003-09--2006-07 大连理工大学 硕士
1998-09--2002-07 大连理工大学 学士

   

2014-07--2015-06 中国科学院大连化学物理研究所 副研究员
2011-05--2014-06 中国科学院大连化学物理研究所 助理研究员

   

（1） 基于亲水作用色谱-串联质谱的人血浆结合珠蛋白糖基化分析研究，主持，部委级，2013-01--2015-12
（2） 糖蛋白与糖链结构的基础与应用研究，参与，部委级，2012-01--2015-12
（3） 糖蛋白糖链结构的基础研究，参与，部委级，2012-01--2016-12

   

[1] 郑天成, 蔡向龙, 李仲慧, 沈陈诚, 刘栋, 刘金波, 郭敬为. Stimulated Raman scattering in CH4 gas using single cylindrical lens focusing. Stimulated Raman scattering in CH4 gas using single cylindrical lens focusing[J]. 2021, 126987-, [2] 高敦峰, 魏鹏飞, 李合肥, 林龙, 汪国雄, 包信和. 用于二氧化碳电催化还原的电解器研究进展（英文）. 物理化学学报. 2021, 126-140, https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFDAUTO&filename=WLHX202105016&v=MTQ4NjRSN3VmWWVSdUZpN2tVNy9PTWlIRGRyRzRITkRNcW85RVlvUjhlWDFMdXhZUzdEaDFUM3FUcldNMUZyQ1U=.
[3] Cuiyan Cao, Long Yu, Jingyu Yan, Dongmei Fu, Jingli Yuan, Xinmiao Liang. Purification of natural neutral N-glycans by using two-dimensional hydrophilic interaction liquid chromatography × porous graphitized carbon chromatography for glycan-microarray assay. TALANTA. 2021, 221: http://dx.doi.org/10.1016/j.talanta.2020.121382.
[4] Cao, Cuiyan, Yu, Long, Zhang, Xiuli, Dong, Xuefang, Yuan, Jingli, Liang, Xinmiao. Calibration for quantitative Fc-glycosylation analysis of therapeutic IgG1-type monoclonal antibodies by using glycopeptide standards. ANALYTICA CHIMICA ACTA[J]. 2021, 1154: http://dx.doi.org/10.1016/j.aca.2021.338306.
[5] Cao, Cuiyan, Yu, Long, Yan, Jingyu, Fu, Dongmei, Yuan, Jingli, Liang, Xinmiao. Purification of natural neutral N-glycans by using two-dimensional hydrophilic interaction liquid chromatography x porous graphitized carbon chromatography for glycan-microarray assay. TALANTA[J]. 2021, 221: https://www.webofscience.com/wos/woscc/full-record/WOS:000579910600007.
[6] 高敦峰, 魏鹏飞, 李合肥, 林龙, 汪国雄, 包信和. 用于二氧化碳电催化还原的电解器研究进展（英文）. 物理化学学报. 2020, 1140K-, [7] Cao, Cuiyan, Yu, Long, Fu, Dongmei, Yuan, Jingli, Liang, Xinmiao. Absolute quantitation of high abundant Fc-glycopeptides from human serum IgG-1. ANALYTICA CHIMICA ACTA[J]. 2020, 1102: 130-139, http://dx.doi.org/10.1016/j.aca.2019.12.035.
[8] Fu, Dongmei, Liu, Yujie, Shen, Aijin, Xiao, Yuansheng, Yu, Long, Liang, Xinmiao. Preparation of glutathione-functionalized zwitterionic silica material for efficient enrichment of sialylated N-glycopeptides. ANALYTICAL AND BIOANALYTICAL CHEMISTRY[J]. 2019, 411(18): 4131-4140, [9] 阎程程, 林龙, 汪国雄, 包信和. 过渡金属-氮活性位点在二氧化碳电化学还原反应中的应用（英文）. 催化学报. 2019, 23-37, https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFDLAST2019&filename=CHUA201901004&v=MjcwNjc0SDlqTXJvOUZZSVI4ZVgxTHV4WVM3RGgxVDNxVHJXTTFGckNVUjdxZVorUnZGaTdsVzd6SkppWGViN0c=.
[10] Zhou, Weijia, Wang, Jixia, Zhao, Yaopeng, Yu, Long, Fang, Ye, Jin, Hongli, Zhou, Han, Zhang, Pengyu, Liu, Yanfang, Zhang, Xiuli, Liang, Xinmiao. Discovery of beta(2)-adrenoceptor agonists in Curcuma zedoaria Rosc using label-free cell phenotypic assay combined with two-dimensional liquid chromatography. JOURNAL OF CHROMATOGRAPHY A[J]. 2018, 1577: 59-65, http://www.corc.org.cn/handle/1471x/2372969.
[11] Yan, Jingyu, Ding, Junjie, Jin, Gaowa, Yu, Dongping, Yu, Long, Lone, Zhen, Guo, Zhimou, Chai, Wengang, Liang, Xinmiao. Profiling of Sialylated Oligosaccharides in Mammalian Milk Using Online Solid Phase Extraction-Hydrophilic Interaction Chromatography Coupled with Negative-Ion Electrospray Mass Spectrometry. ANALYTICAL CHEMISTRY[J]. 2018, 90(5): 3174-3182, http://cas-ir.dicp.ac.cn/handle/321008/168864.
[12] Zhou, Weijia, Guo, Zhimou, Yu, Long, Zhou, Han, Shen, Aijin, Jin, Yan, Jin, Gaowa, Yan, Jingyu, Yang, Fan, Liu, Yanming, Wang, ChaoRan, Feng, JiaTao, Liu, Yanfang, Liang, Xinmiao. On-line comprehensive two-dimensional liquid chromatography tandem mass spectrometry for the analysis of Curcuma kwangsiensis. TALANTA[J]. 2018, 186: 73-79, http://dx.doi.org/10.1016/j.talanta.2018.04.014.
[13] Dong, Xuefang, Qin, Hongqiang, Mao, Jiawei, Yu, Dongping, Li, Xiuling, Shen, Aijin, Yan, Jingyu, Yu, Long, Guo, Zhimou, Ye, Mingliang, Zou, Hanfa, Liang, Xinmiao. In-Depth Analysis of Glycoprotein Sialylation in Serum Using a Dual-Functional Material with Superior Hydrophilicity and Switchable Surface Charge. ANALYTICAL CHEMISTRY[J]. 2017, 89(7): 3966-3972, http://cas-ir.dicp.ac.cn/handle/321008/169302.
[14] 胡墅, 盖宝栋, 郭敬为, 王鹏远, 李学杨, 李慧, 刘金波, 何山, 蔡向龙, 刘栋, 陈莹, 桑凤亭, 金玉奇. Population inversion in sodium D2 transition based on sodium-ethane excimer pairs. 中国光学快报：英文版[J]. 2017, 15(11): 56-60, http://lib.cqvip.com/Qikan/Article/Detail?id=673835845.
[15] Cao, Liwei, Yu, Long, Guo, Zhimou, Shen, Aijin, Guo, Yunu, Liang, Xinmiao. N-Glycosylation Site Analysis of Proteins from Saccharomyces cerevisiae by Using Hydrophilic Interaction Liquid Chromatography-Based Enrichment, Parallel Deglycosylation, and Mass Spectrometry. JOURNAL OF PROTEOME RESEARCH[J]. 2014, 13(3): 1485-1493, http://www.irgrid.ac.cn/handle/1471x/858785.
[16] Cao, Liwei, Zhang, Ye, Chen, Linlin, Shen, Aijin, Zhang, Xingwang, Ren, Shifang, Gu, Jianxin, Yu, Long, Liang, Xinmiao. Sample preparation for mass spectrometric analysis of human serum N-glycans using hydrophilic interaction chromatography-based solid phase extraction. ANALYST[J]. 2014, 139(18): 4538-4546, http://cas-ir.dicp.ac.cn/handle/321008/144355.
[17] Xu, Junyan, Zhang, Xiuli, Guo, Zhimou, Yan, Jingyu, Yu, Long, Li, Xiuling, Xue, Xingya, Liang, Xinmiao. Orthogonal separation and identification of long-chain peptides from scorpion Buthus martensi Karsch venom by using two-dimensional mixed-mode reversed phase-reversed phase chromatography coupled to tandem mass spectrometry. ANALYST[J]. 2013, 138(6): 1835-1843, http://www.irgrid.ac.cn/handle/1471x/721017.
[18] 赵旭, 姜武辉, 于龙, 邹丽娟, 李秀玲, 梁鑫淼. 氧化铝富集糖肽的研究. 化学学报[J]. 2013, 71(3): 343-, http://www.irgrid.ac.cn/handle/1471x/720955.
[19] Cao, Liwei, Yu, Long, Guo, Zhimou, Li, Xiuling, Xue, Xinya, Liang, Xinmiao. Application of a strong anion exchange material in electrostatic repulsion-hydrophilic interaction chromatography for selective enrichment of glycopeptides. JOURNALOFCHROMATOGRAPHYA[J]. 2013, 1299: 18-24, http://dx.doi.org/10.1016/j.chroma.2013.05.037.
[20] Xu, Junyan, Zhang, Xiuli, Guo, Zhimou, Yan, Jingyu, Yu, Long, Li, Xiuling, Xue, Xingya, Liang, Xinmiao. Short-chain peptides identification of scorpion Buthus martensi Karsch venom by employing high orthogonal 2D-HPLC system and tandem mass spectrometry. PROTEOMICS[J]. 2012, 12(19-20): 3076-3084, http://www.irgrid.ac.cn/handle/1471x/721404.
[21] 于龙, 李秀玲, 侯美倩, 张秀莉, 梁鑫淼. 人血清Alpha-1-酸性糖蛋白的糖基化分析. 中国科学：化学[J]. 2011, 41(3): 544-549, http://lib.cqvip.com/Qikan/Article/Detail?id=37020405.
[22] 蔡晓明, 张岩, 于龙, 郭志谋, 张秀莉, 梁鑫淼. 高效亲和色谱法测定2种中药成分与人血清白蛋白的结合率. 色谱[J]. 2011, 29(4): 358-361, http://lib.cqvip.com/Qikan/Article/Detail?id=37561910.