杨晟 博导 中国科学院分子植物科学卓越创新中心
电子邮件 syang@sibs.ac.cn
通信地址 上海市枫林路300号2号楼1101
微生物遗传与工程生物学服务号
课题组简况
新一代DNA测序与低成本DNA合成技术提供了海量基因资源与快速廉价的基因获取手段，为设计和创制工程细胞提供了近乎无限的可能性。我们实验室开发基于CRISPR相关系统的微生物基因组编辑工具和定向进化方法，加速细胞构建以理解相关遗传与生化机制，据此迭代设计工程微生物，用于能源、农业和医药领域。
实验室设计的基因组编辑工具质粒分发四千余次，数十种生物催化酶与化学品高产菌株在国内商用，纤维素乙醇酵母在中国、美国与巴西应用于二代乙醇生产。杨晟研究员获DuPont Young Professor Award, Novozymes Innovation Award，国务院政府特殊津贴及国家杰出青年科学基金。多位毕业生创办或联合创办各自的合成生物学公司。
本组现有助理5人，博后1人，博士生3人，硕士生1人及一批客座研发人员。
实验室下一阶段将运用大语言模型生成蛋白质乃至基因组，借助基因组合成与多重基因组编辑技术，结合系统生物学与定向进化创制新一代工程细胞，通过细胞集群与分离耦合策略，将生物制造效率提升到极致。
招新启事
1. 硕博连读生（微生物学）
每年两名含博士生。
建议大三暑期前早联系早实习早了解早决定早选择。
2. 外招博士生
每位导师最多推荐3名学生参加笔试，笔试通过后，最多推荐1名学生参加面试，最后根据总分从高到低排序，待硕士研究生4月中旬完成转博后，再根据剩余博士名额及同济大学和华东理工大学联培名额进行录取。
博士研究生为申请-考核制，《基础生物学》由我单位统一组织考试，满分100分，考试时间1小时，业务课二由课题组组织考核（形式不限，面试、实验操作等）并打分（共三档：A-90分，B-80分，C-70分）。
报考上海交通大学、复旦大学和浙江大学联合培养博士生需在这些学校系统上报名。
3. 专业硕士生（生物与医药）
每年1名，没想好是否读博的可以考虑。往年基本没啥竞争，推免生或上线统考生先到先得。
4. 特别研究助理/博士后
职责为完成高水平论文和/或开展企业合作项目。享受上海市落户绿色通道政策，年薪按单位标准，就高不就低。
5. 中国科学技术大学"所系结合”联合培养硕博连读生
5.1 中国科学技术大学（中科大）依托学院负责组织基地研究生招生，将基地介绍和招生专业纳入本学院招生简章和专业目录，统筹安排招生计划，组织复试录取工作，邀请基地专家参与招生选拔工作。
5.2 基地研究生纳入中科大学籍管理。基地研究生第一学年在校本部完成基础课程学习，第二学年开始进入研究所开展科研训练。
5.3 基地硕士研究生可申请转博，淘汰率约50%。
5.4 基地研究生学位申请和授予按照中科大相关学科研究生学位授予标准执行。（博士毕业要求：a，中科大为第一署名单位；b 独立一作或并列一作的前两位。独立一作文章的水平大概在3-5分的杂志，并列作者文章大概 Plant Cell及以上。硕士毕业要求：一篇第一作者文章（杂志不限）或专利）
5.5 基地研究生在所系结合研究生导师指导下取得的科研成果，其知识产权原则上由我校和基地所在科研院所共享， 发表学术论文(著作)、申请专利、申报奖项等成果可署名双方单位，用以申请学位的科研成果必须以“中国科学技术大学”为 第一署名(完成)单位。
5.6 所系结合研究生导师负责提供基地研究生在学期间的助研津贴，在基地学习期间的资助标准不低于基地所在科研院所研究生助研津贴标准。
6.实验室科研相关活动
不限于
每周一次一对一office hour（基于周报）
每两周一次组会
每月一次文献解读
每周一篇文献摘译，优秀摘译刊登在“微生物遗传与工程生物学”微信服务号
不定期科研思维训练
不定期写作训练
不定期职业规划辅导
不定期企业交流
不定期读书交流
7.招生信息
071005-微生物学
毕业去向
发表论文
论文详见https://www.researchgate.net/profile/Sheng_Yang10
主通讯论文
1. Siqi Yang, Fenghui Qian, Tao Wu, Bingbing Sun, Huiqi He, Meng Qiao, Feng Dong, Peng Gao, Zhao Zhen, Ying Zhang, Junjie Yang, Yu Jiang*, Sheng Yang*. Anaerobic metabolic evolution for homotypic L-valine fermentation. Nature Communications, 2026, 17, 6996. doi:10.1038/s41467-026-73619-7
2. Xiaojie Zhou#, Yu Jiang#,*, Bingbing Sun#, Feng Dong, Sheng Yang*. De novo engineering of Escherichia coli for an L-lysine hyperproducer. Journal of Agricultural and Food Chemistry, 2026, 74, 22, 17252–17259. doi:10.1021/acs.jafc.5c17165
3. Xiaoyu Wang, Siqi Yang, Xiaojie Zhou, Jiao Zhu, Fenghui Qian, Yu Jiang*, Sheng Yang*. One-step multi-fragment assembly and targeted genomic integration in Corynebacterium glutamicum. ACS Synthetic Biology, 2026, 15(3):1073-1081. doi:10.1021/acssynbio.5c00752
4. Jiao Zhu, Jiao Zhang, Feng Dong, Rongsheng Tao, Junjie Yang, Yingmiao Liu, Ying Zhang, Yu Jiang*, Sheng Yang*. Reverse metabolic engineering of L-arginine biosynthesis in Corynebacterium glutamicum. ACS Sustainable Chemistry & Engineering, 2025, 13(39):16248-16256. doi:10.1021/acssuschemeng.5c03737
5. Xiaoyu Wang, Siqi Yang, Fenghui Qian, Feng Dong, Xiaojie Zhou, Mingyu Yin, Ying Zhang, Zhiwei Huang, Yu Jiang, Sheng Yang*. RAGATH-associated DNA nuclease assisted DNA Insertion in Corynebacterium glutamicum. ACS Synthetic Biology, 2025, 14(5):1861-1867. doi:10.1021/acssynbio.5c00022
6. Xiaojie Zhou, Siqi Yang, Bingbing Sun, Feng Dong, Mingyu Yin, Yu Jiang, Zhiwei Huang, Sheng Yang*. Implementation of RAGATH RNA-associated DNA endonucleases as genome editing tool in Escherichia coli. Biotechnology Journal, 2025, 20(3):e70005. doi:10.1002/biot.70005
7. Kaiqi Zhi, Zhiguo An, Mingyang Zhang, Kehan Liu, Yafan Cai, Zhenyu Wang, Di Zhang, Jinle Liu, Zhi Wang, Chenjie Zhu, Dong Liu*, Sheng Yang*, Hanjie Ying. Biofilm-based immobilization fermentation for continuous hEGF production in Saccharomyces cerevisiae. Fermentation, 2024, 10(12):661. doi:10.3390/fermentation10120661
8. Yiwen Zhang#, Junjie Yang#, Fenghui Qian, Kate Brandon Sutton, Carsten Hjort, Wenping Wu, Yu Jiang, Sheng Yang*. Engineering a xylose fermenting yeast for lignocellulosic ethanol production. Nature Chemical Biology, 2025, 21(3):443-450. doi:10.1038/s41589-024-01771-6
9. Ping Yang, Junjie Yang, Ting Lin, Qi Liu, Yu Yin, Daijie Chen, Sheng Yang*. Efficient genome editing in most Staphylococcus aureus by using the restriction-modification system silent CRISPR-Cas9 toolkit. ACS Synthetic Biology, 2023, 12(11):3340-3351. doi:10.1021/acssynbio.3c00339
10. Jinle Liu, Junjie Yang, Lihua Yuan, Chunhua Wu, Yu Jiang, Wei Zhuang, Hanjie Ying, Sheng Yang*. Modulated arabinose uptake and cAMP signaling synergistically improve glucose and arabinose consumption in recombinant yeast. Journal of Agricultural and Food Chemistry, 2023, 71(34):12797-12806 doi:10.1021/acs.jafc.3c04386
11. Siqi Yang, Jiao Zhu, Xiaojie Zhou, Jiao Zhang, Qi Li, Furong Bian, Jidong Zhu, Ting Yan, Xiaoyu Wang, Ying Zhang, Junjie Yang, Yu Jiang, Sheng Yang*. RNA-guided DNA transposition in Corynebacterium glutamicum and Bacillus subtilis. ACS Synthetic Biology, 2023, 12(7):2198-2202. doi:10.1021/acssynbio.3c00193
12. Yu Jiang#, Xiyuan Li#, Fenghui Qian, Bingbing Sun, Xiyuan Wang, Yan Zhang, Deqiang Zhang, Meiyu Geng, Zuoquan Xie*, Sheng Yang*. Fine-tuning bacterial cyclic di-AMP production for durable antitumor effects through the activation of the STING pathway. Research, 2023, 6:0102. doi:10.34133/research.0102
13. Qi Li, Mingjun Sun, Lu Lv, Yong Zuo, Suyi Zhang, Ying Zhang, Sheng Yang*. Improving the editing efficiency of CRISPR-Cas9 by reducing the generation of escapers based on the surviving mechanism. ACS Synthetic Biology, 2023, 12(3):672-680. doi:10.1021/acssynbio.2c00619
14. Zhiqiang Wen, Jun Chen, Yunliu Yang, Ying Zhang, Yu Jiang*, Sheng Yang*. Pilot-scale fermentation of 300t/a total solvents from sweet sorghum stalk juice. Chemical Engineering Journal, 2023, 454:140534. doi:10.1016/j.cej.2022.140534
15. Yu Jiang#*, Bingbing Sun#, Fenghui Qian, Feng Dong, Chongmao Xu, Wuling Zhong, Rui Huang, Qiwei Zhai, Sheng Yang*. Expression of phenylalanine ammonia lyase as an intracellularly free and extracellularly cell surface-immobilized enzyme on a gut microbe as a living therapeutic for phenylketonuria. Science China Life Sciences, 2023, 66(1):127-136. doi:10.1007/s11427-021-2137-3
16. Qingzhuo Wang#, Huabao Zheng#, Rongsheng Tao, Qi Li, Yu Jiang*, Sheng Yang*. Vitreoscilla hemoglobin enhances the catalytic performance of industrial oxidases in vitro. Applied Microbiology and Biotechnology, 2022, 106(9-10):3657-3667. doi:10.1007/s00253-022-11974-3
17. Junjie Yang#*, Jiawei Yang#, Yiwen Zhang, Siqi Yang, Jieze Zhang, Yu Jiang, Sheng Yang*. CRISPR-associated transposase system can insert multiple copies of donor DNA into the same target locus. The CRISPR Journal, 2021, 4(6):789-798. 10.1089/crispr.2021.0019
18. Qi Li, Jieze Zhang, Junjie Yang, Yu Jiang, Sheng Yang*. Recent progress on n-butanol production by lactic acid bacteria. World Journal of Microbiology and Biotechnology, 2021, 37(12): 205. doi:10.1007/s11274-021-03173-5
19. Siqi Yang#, Yiwen Zhang#, Jiaqi Xu, Jiao Zhang, Jieze Zhang, Junjie Yang, Yu Jiang, Sheng Yang*. Orthogonal CRISPR-associated transposases for parallel and multiplexed chromosomal integration. Nucleic Acids Research, 2021, 49(17): 10192-10202. doi:10.1093/nar/gkab752
20. Yiwen Zhang, Jiawei Yang, Siqi Yang, Jieze Zhang, Jun Chen, Rongsheng Tao, Yu Jiang, Junjie Yang, Sheng Yang*. Programming cells by multicopy chromosomal integration using CRISPR-associated transposases. The CRISPR Journal, 2021, 4(3): 350-359. doi:10.1089/crispr.2021.0018
21. Jiaqi Xu, Junjie Yang, Yu Jiang, Mianbin Wu*, Sheng Yang*, Lirong Yang. A novel global transcriptional perturbation target identified by forward genetics reprograms Vibrio natriegens for improving recombinant protein production. Acta Biochimica et Biophysica Sinica, 2021, 53(9): 1124-1133. doi:10.1093/abbs/gmab089
22. Tianqiong Shi, Yawen Li, Li Zhu, Yangyang Tong, Junjie Yang, Yunming Fang, Meng Wang, Jieze Zhang, Yu Jiang, Sheng Yang*. Engineering the oleaginous yeast Yarrowia lipolytica for β-farnesene overproduction. Biotechnology Journal, 2021, 16(7): 2100097. doi:10.1002/biot.202100097
23. Li Zhu, Jieze Zhang, Jiawei Yang, Yu Jiang, Sheng Yang*. Strategies for optimizing acetyl-CoA formation from glucose in bacteria. Trends in Biotechnology, 2022, 40(2): 149-165. doi:10.1016/j.tibtech.2021.04.004
24. Qi Li, Bingbing Sun, Jun Chen, Yiwen Zhang, Yu Jiang, Sheng Yang*. A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing in Escherichia coli. Acta Biochimica et Biophysica Sinica, 2021, 53(5): 620-627. doi:10.1093/abbs/gmab036
25. Xin Wang, Bei Liao, Zhijun Li, Guangxin Liu, Liuyang Diao, Fenghui Qian, Junjie Yang, Yu Jiang, Shumiao Zhao, Youguo Li, Sheng Yang*. Reducing glucoamylase usage for commercial-scale ethanol production from starch using glucoamylase expressing Saccharomyces cerevisiae. Bioresources and Bioprocessing, 2021, 8(1): 20. doi:10.1186/s40643-021-00375-5
26. Qi Li, Meixian Wu, Zhiqiang Wen, Yuan Jiang, Xin Wang, Yawei Zhao, Jinle Liu, Junjie Yang, Yu Jiang, Sheng Yang*. Optimization of n-butanol synthesis in Lactobacillus brevis via the functional expression of thl, hbd, crt and ter. Journal of Industrial Microbiology & Biotechnology, 2020, 47(12): 1099-1108. doi:10.1007/s10295-020-02331-2
27. Xiaoman Sun, Zixu Zhang, Lingru Wang, Jinggang Wang, Yan Liang, Haifeng Yang, Rongsheng Tao, Yu Jiang, Junjie Yang, Sheng Yang*. Downregulation of T7 RNA polymerase transcription enhances pET-based recombinant protein production in Escherichia coli BL21 (DE3) by suppressing autolysis, Biotechnology and Bioengineering, 2020, 117(11): 3389-3398. doi:10.1002/bit.27558
28. Jiao Zhang, Fenghui Qian, Feng Dong, Qingzhuo Wang, Junjie Yang, Yu Jiang*, Sheng Yang*. De novo engineering of Corynebacterium glutamicum for L-proline production. ACS Synthetic Biology, 2020, 9(11):2987-2996. doi:10.1021/acssynbio.0c00249
29. Yiwen Zhang, Xiaoman Sun, Qingzhuo Wang, Jiaqi Xu, Feng Dong, Siqi Yang, Jiawei Yang, Zixu Zhang, Yuan Qian, Jun Chen, Jiao Zhang, Yingmiao Liu, Rongsheng Tao, Yu Jiang, Junjie Yang*, Sheng Yang*. Multicopy chromosomal integration using CRISPR-associated transposases. ACS Synthetic Biology, 2020, 9(5):1189-1198. doi:10.1021/acssynbio.0c00073
30. Jinle Liu, Yu Jiang, Jun Chen, Junjie Yang, Weihong Jiang, Wei Zhuang, Hanjie Ying, Sheng Yang*. Metabolic engineering and adaptive evolution of Clostridium beijerinckii to increase solvent production from corn stover hydrolysate. Journal of Agricultural and Food Chemistry, 2020, 68(32):8678-8687. doi:10.1021/acs.jafc.0c03048
31. Zhiqiang Wen, Qi Li, Jinle Liu, Mingjie Jin*, Sheng Yang*. Consolidated bioprocessing for butanol production of cellulolytic clostridia: development and optimization. Microbial Biotechnology, 2020, 13(2):410-422. doi: 10.1111/1751-7915.13478
32. Zhiqiang Wen, Minrui Lu, Rodrigo Ledesma-Amaro, Qi Li, Mingjie Jin*, Sheng Yang*. Targetron technology applicable in solventogenic Clostridia: Revisiting 12 Years’ Advances. Biotechnology Journal, 2020, 15(1):e1900284. doi:10.1002/biot.201900284
33. Zhiqiang Wen, Rodrigo Ledesma-Amaro, Minrui Lu, Yu Jiang, Shuliang Gao, Mingjie Jin*, Sheng Yang*. Combined evolutionary engineering and genetic manipulation improve low ph tolerance and butanol production in a synthetic microbial Clostridium community. Biotechnology and Bioengineering, 2020, 117(8):2456-2465. doi:10.1002/bit.27333
34. Zhiqiang Wen, Rodrigo Ledesma-Amaro, Minrui Lu, Mingjie Jin*, Sheng Yang*. Metabolic engineering of Clostridium cellulovorans to Improve Butanol Production by Consolidated Bioprocessing. ACS Synthetic Biology, 2020, 9(2):412-421. doi:10.1021/acssynbio.9b00331
35. Qi Li, Francois M. Seys, Nigel P. Minton, Junjie Yang, Yu Jiang, Weihong Jiang, Sheng Yang*. CRISPR-Cas9D10A nickase-assisted base editing in the solvent producer Clostridium beijerinckii. Biotechnology and Bioengineering, 2019, 116(10):2645-2654. doi:10.1002/bit.26949
36. He Huang, Xin Song, Sheng Yang*. Development of RecE/T-assisted CRISPR-Cas9 toolbox for Lactobacillus. Biotechnology Journal, 2019, 14(5):1800690. doi:10.1002/biot.201800690
37. Zhiqiang Wen, Rodrigo Ledesma-Amaro, Jianping Lin*, Yu Jiang, Sheng Yang*. Improved n-butanol production from Clostridium cellulovorans by Integrated Metabolic and Evolutionary Engineering. Applied and Environmental Microbiology, 2019, 85(6):e02560-18. doi:10.1128/AEM.02560-18
38. 李琦，武美贤，郭清华，邵悠然，杨俊杰，蒋宇*, 杨晟*. 细菌基因组编辑技术进展。生命科学，2018, 30 (05):473-492. doi:10.13376/j.cbls/2019201
39. Jun Sun, Qingzhuo Wang, Yu Jiang, Zhiqiang Wen, Lirong Yang, Jianping Wu*, Sheng Yang*. Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system. Microbial Cell Factories, 2018, 17(1):41. doi:10.1186/s12934-018-0848-5
40. Xin Song, He Huang, Zhi-Qiang Xiong, Lianzhong Ai*, Sheng Yang*. CRISPR-Cas9D10A nickase-assisted genome editing in Lactobacillus casei. Applied and Environmental Microbiology, 2017, 83(22):e01259-17. doi:10.1128/AEM.01259-17
41. Xiaoshu Xu, Chao Wang, Jun Chen, Sheng Yang*. Streptomyces virginiae PPDC is a new type of phenylpyruvate decarboxylase composed of two subunits. ACS Chemical Biology, 2017, 12(8):2008-2014. doi:10.1021/acschembio.7b00307
42. Yu Jiang, Fenghui Qian, Junjie Yang, Yingmiao Liu, Feng Dong, Chongmao Xu, Bingbing Sun, Biao Chen, Xiaoshu Xu, Yan Li, Renxiao Wang, Sheng Yang*. CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum. Nature Communications, 2017, 8(1):15179. doi:10.1038/ncomms15179（ESI高被引）
杨晟研究组建立高效的谷氨酸棒杆菌CRISPR-Cpf1基因组编辑系统
43. Shuliang Gao, Yangyang Tong, Li Zhu, Mei Ge, Yian Zhang, Daijie Chen, Yu Jiang, Sheng Yang*. Iterative integration of multiple-copy pathway genes in Yarrowia lipolytica for heterologous β-carotene production. Metabolic Engineering, 2017, 41:192-201. doi:10.1016/j.ymben.2017.04.004
44. Zhiqiang Wen, Nigel P. Minton, Ying Zhang, Qi Li, Jinle Liu, Yu Jiang, Sheng Yang*. Enhanced solvent production by metabolic engineering of a twin-clostridial consortium. Metabolic Engineering, 2017, 39:38-48. doi:10.1016/j.ymben.2016.10.013
45. Qingzhuo Wang, ShuangYan Tang*, Sheng Yang*. Genetic biosensors for small-molecule products: design and applications in high-throughput screening. Frontiers of Chemical Science and Engineering, 2017, 11(1):15-26. doi:10.1007/s11705-017-1629-z
46. Junjie Yang, Sheng Yang*. Comparative analysis of Corynebacterium glutamicum genomes: a new perspective for the industrial production of amino acids. BMC Genomics, 2017, 18(1):940. doi:10.1186/s12864-016-3255-4
47. Yu Jiang, Rongsheng Tao, Zhengquan Shen, Liangdong Sun, Fuyun Zhu, Sheng Yang*. Enzymatic production of glutathione by gifunctional γ-glutamylcysteine synthetase/glutathione synthetase coupled with in vitro acetate kinase-based ATP generation. Applied Biochemistry and Biotechnology, 2016, 180(7):1446-1455. doi:10.1007/s12010-016-2178-5
48. Shuliang Gao, Yangyang Tong, Zhiqiang Wen, Li Zhu, Mei Ge, Daijie Chen*, Yu Jiang*, Sheng Yang*. Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system. Journal of Industrial Microbiology and Biotechnology, 2016, 43(8):1085-1093. doi:10.1007/s10295-016-1789-8
49. Yu Jiang, Rongsheng Tao, Sheng Yang*. Tailor-made biocatalysts enzymes for the fine chemical industry in China. Biotechnology Journal, 2016, 11(10):1121-1123. doi:10.1002/biot.201500682
50. Qi Li, Jun Chen, Nigel P. Minton, Ying Zhang, Zhiqiang Wen, Jinle Liu, Haifeng Yang, Zhe Zeng, Xiaodan Ren, Junjie Yang, Yang Gu, Weihong Jiang, Yu Jiang, Sheng Yang*. CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii. Biotechnology Journal, 2016, 11(7):961-972. doi:10.1002/biot.201600053
51. Chen Yang#, Xiang Gao#, Yu Jiang#, Bingbing Sun#, Fang Gao, Sheng Yang*. Synergy between methylerythritol phosphate pathway and mevalonate pathway for isoprene production in Escherichia coli. Metabolic Engineering, 2016, 37:79-91. doi:10.1016/j.ymben.2016.05.003
52. Xiaoshu Xu, Jun Chen, Qingzhuo Wang, Chunlan Duan, Yan Li, Renxiao Wang, Sheng Yang*. Mutagenesis of key residues in the binding center of L-aspartate-β-semialdehyde dehydrogenase from Escherichia coli enhances utilization of the cofactor NAD(H). ChemBioChem, 2016, 17:56-64. doi:10.1002/cbic.201500534
53. Yu Jiang, Biao Chen, Chunlan Duan, Bingbing Sun, Junjie Yang, Sheng Yang*. Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system. Applied and Environmental Microbiology, 2015, 81(7):2506-2514. doi:10.1128/AEM.03802-14（ESI高被引）
54. Ning Zhang#, Lijun Shao#, Yu Jiang, Yang Gu, Qi Li, Jinle Liu, Weihong Jiang, Sheng Yang*. I-SceI-mediated scarless gene modification via allelic exchange in Clostridium. Journal of Microbiological Methods, 2015, 108:49-60. doi:10.1016/j.mimet.2014.11.004
55. Yu Jiang, Jinle Liu, Yunliu Yang, Weihong Jiang, Sheng Yang*. Current status and prospects of industrial bio-production of n-butanol in China. Biotechnology Advances, 2014, 33(7):1493-1501. doi:10.1016/j.biotechadv.2014.10.007
56. Junjie Yang, Bingbing Sun, He Huang, Yu Jiang, Liuyang Diao, Biao Chen, Chongmao Xu, Xin Wang, Jinle Liu, Weihong Jiang, Sheng Yang*. High-efficiency scarless genetic modification in Escherichia coli. Applied and Environmental Microbiology, 2014, 80(13):3826-3834. doi:10.1128/AEM.00313-14
57. Rongsheng Tao, Yu Jiang, Fuyun Zhu, Sheng Yang*. A One-pot system for production of L-2-aminobutyric acid from L-threonine by L-threonine deaminase and a NADH-regeneration system based on L-leucine dehydrogenase and formate dehydrogenase. Biotechnology Letters, 2014, 36(4):835-841. doi:10.1007/s10529-013-1424-y
58. Liuyang Diao, Yingmiao Liu, Fenghui Qian, Junjie Yang, Yu Jiang, Sheng Yang*. Construction of fast xylose-fermenting yeast based on industrial ethanol-producing diploid Saccharomyces cerevisiae by rational design and adaptive evolution. BMC Biotechnology, 2013, 13(1):110. doi:10.1186/1472-6750-13-110
59. Zhilin Li, Han Xiao, Weihong Jiang, Yu Jiang*, Sheng Yang*. Improvement of solvent production from xylose mother liquor by engineering the xylose metabolic pathway in Clostridium acetobutylicum EA2018. Applied Biochemistry and Biotechnology, 2013, 171(3):555-568. doi:10.1007/s12010-013-0414-9
60. Han Xiao, Zhilin Li, Yu Jiang, Yunliu Yang, Weihong Jiang, Yang Gu*, Sheng Yang*. Metabolic engineering of D-xylose pathway in Clostridium Beijerinckii to optimize solvent production from xylose mother liquid. Metabolic Engineering, 2012, 14(5):565-578. doi:10.1016/j.ymben.2012.05.003
61. Han Xiao#, Yang Gu#, Yuanyuan Ning, Yunliu Yang, W.J. Mitchell, Weihong Jiang*, Sheng Yang*. Confirmation and elimination of xylose metabolism bottlenecks in glucose phosphoenolpyruvate-dependent phosphotransferase system-deficient Clostridium acetobutylicum for simultaneous utilization of glucose, xylose, and arabinose. Applied and Environmental Microbiology, 2011, 77(22):7886-7895. doi:10.1128/aem.00644-11-11
62. Zhoutong Sun, Yuanyuan Ning, Lixia Liu, Yingmiao Liu, Bingbing Sun, Weihong Jiang, Chen Yang, Sheng Yang*. Metabolic engineering of the L-phenylalanine pathway in Escherichia coli for the production of S- or R-mandelic acid. Microbial Cell Factories, 2011, 10(1):71. doi:10.1186/1475-2859-10-71
63. Li Zhu, Rongsheng Tao, Yi Wang, Yu Jiang, Xin Lin, Yunliu Yang, Huabao Zheng, Weihong Jiang, Sheng Yang*. Removal of L-alanine from the production of L-2-aminobutyric acid by introduction of alanine racemase and D-amino acid oxidase. Applied Microbiology and Biotechnology, 2011, 90(3):903-910. doi:10.1007/s00253-011-3127-4
64. Jian Li, Jingbo Zhao, Meng Zhao, Yunliu Yang, Weihong Jiang, Sheng Yang*. Screening and characterization of butanol-tolerant micro-organisms. Letters in Applied Microbiology, 2010, 50(4):373-379. doi:10.1111/j.1472-765X.2010.02808.x
65. Yu Jiang, Chongmao Xu, Feng Dong, Yunliu Yang, Weihong Jiang*, Sheng Yang*. Disruption of the acetoacetate decarboxylase gene in solvent-producing Clostridium acetobutylicum increases the butanol ratio. Metabolic Engineering, 2009, 11(4):284-291. doi:10.1016/j.ymben.2009.06.002
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