Antimicrobial resistance and therapy;

Bacteriophage lysin;

Novel antimicrobial discovery;

Bacteriophages and host immunity;

2010/09-2013/06   Ph. D. in Microbiology.

Wuhan Institute of Virology, CAS, Wuhan, China.

2007/09-2010/06   M. D. in Fermentation Engineering.

Huazhong Agricultural University, Wuhan, China.

2003/09-2007/06   B. S. in Biological Engineering.

Wuhan Polytechnic University, Wuhan, China.

  2021/10-                 Professor，

                 Wuhan Institute of Virology, CAS, Wuhan, China.

 2018/01- 2021/10      Associate Professor，

                 Wuhan Institute of Virology, CAS, Wuhan, China.

2018/04- 2019/04       Visiting Assistant Research Scientist，

                 University of Maryland, College Park, MD, USA.

   2015/08- 2017/12     Associate Professor,

Wuhan Institute of Virology, CAS, Wuhan, China.

2013/07-2015/08    Assistant Professor,

Wuhan Institute of Virology, CAS, Wuhan, China.

2021

1.        Li X, Wang S, Nyaruaba R, Liu H, Yang H*, Wei H*. 2021. A Highly Active Chimeric Lysin with a Calcium-Enhanced Bactericidal Activity against Staphylococcus aureus In Vitro and In Vivo. Antibiotics (Basel) 10.

2.        Dai W^#, Jochmans D^#, Xie H^#, Yang H^#, Li J, Su H, Chang D, Wang J, Peng J, Zhu L, Nian Y, Hilgenfeld R, Jiang H, Chen K, Zhang L*, Xu Y*, Neyts J*, Liu H*. 2021. Design, Synthesis, and Biological Evaluation of Peptidomimetic Aldehydes as Broad-Spectrum Inhibitors against Enterovirus and SARS-CoV-2. J Med Chem doi:10.1021/acs.jmedchem.0c02258.

3.        Yang H, Xu J, Gong Y, Tang Y, Li W, Zheng Z, Li Y, He J, Wei H*. 2021. Internal cell-penetrating peptide-mediated internalization enables a chimeric lysin to target intracellular pathogens. Int J Pharm 599:120449.

4.        Khan FM, Gondil VS, Li C, Jiang M, Li J, Yu J, Wei H*, Yang H*. 2021. A Novel Acinetobacter baumannii Bacteriophage Endolysin LysAB54 With High Antibacterial Activity Against Multiple Gram-Negative Microbes. Front Cell Infect Microbiol 11:637313.

2020

1.        Luo D, Huang L, Gondil VS, Zhou W, Yang W, Jia M, Hu S, He J*, Yang H*, Wei H*. 2020. A Choline-Recognizing Monomeric Lysin, ClyJ-3m, Shows Elevated Activity against Streptococcus pneumoniae. Antimicrob Agents Chemother 64.

2.        Wang Y, Khanal D, Yoon Kyung Chang R, Shang X, Yang H, Britton WJ, Nelson D, Chan HK. 2020. Can bacteriophage endolysins be nebulised for inhalation delivery against Streptococcus pneumoniae? Int J Pharm doi:10.1016/j.ijpharm.2020.119982:119982.

3.        Huang L, Luo D, Gondil VS, Gong Y, Jia M, Yan D, He J, Hu S*, Yang H*, Wei H*. 2020. Construction and characterization of a chimeric lysin ClyV with improved bactericidal activity against Streptococcus agalactiae in vitro and in vivo. Appl Microbiol Biotechnol 104:1609-1619.

4.        Yang H, Luo D, Etobayeva I, Li X, Gong Y, Wang S, Li Q, Xu P, Yin W, He J*, Nelson DC*, Wei H*. 2020. Linker Editing of Pneumococcal Lysin ClyJ Conveys Improved Bactericidal Activity. Antimicrob Agents Chemother 64.

2019

1. Yang H, Gong Y, Zhang H, Etobayeva I, Miernikiewicz P, Luo D, Li X, Zhang X, Dabrowska K, Nelson DC^*, He J^*, Wei H^*. 2019. ClyJ is a novel pneumococcal chimeric lysin with a cysteine- and histidine-dependent amidohydrolase/peptidase catalytic domain. Antimicrob Agents Chemother

2018

   1. Yang H, Xu J, Li W, Wang S, Li J, Yu J, Li Y^*, Wei H^*. 2018. Staphylococcus aureus virulence attenuation and immune clearance mediated by a phage lysin-derived protein. EMBO J 37.

   2．Li W^#, Yang H^#, Gong Y, Wang S, Li Y^*, Wei H^*. 2018. Effects of a Chimeric Lysin against Planktonic and Sessile Enterococcus faecalis Hint at Potential Application in Endodontic Therapy. Viruses 10.

  3. Xu J^#, Yang H^#, Bi Y, Li W, Wei H, Li Y^*. 2018. Activity of the Chimeric Lysin ClyR against Common Gram-Positive Oral Microbes and Its Anticaries Efficacy in Rat Models. Viruses 10.

2010-2017

1.        Yang H, Zhang H, Wang J, Yu J, Wei H^*. 2017. A novel chimeric lysin with robust antibacterial activity against planktonic and biofilm methicillin-resistant Staphylococcus aureus. Sci Rep 7:40182.

2.         Yang H^#, Bi Y^#, Shang X, Wang M, Linden SB, Li Y, Li Y, Nelson DC^*, Wei H^*. 2016. Anti-biofilm activities of a novel chimeolysin against S. mutans in physiological and cariogenic conditions. Antimicrob Agents Chemother doi:10.1128/AAC.01872-16.

3.         Yang H^#, Wang M^#, Yu J, Wei H^*. 2015. Antibacterial Activity of a Novel Peptide-Modified Lysin Against Acinetobacter baumannii and Pseudomonas aeruginosa. Front Microbiol 6:1471.

4.         Yang H, Wang M, Yu J, Wei H^*. 2015. Aspartate inhibits Staphylococcus aureus biofilm formation. FEMS Microbiol Lett 362.

5.         Yang H, Linden SB, Wang J, Yu J, Nelson DC^*, Wei H^*. 2015. A chimeolysin with extended-spectrum streptococcal host range found by an induced lysis-based rapid screening method. Sci Rep 5:17257.

6.         Huang Y^#, Yang H^#, Yu J, Wei H^*. 2015. Molecular dissection of phage lysin PlySs2: integrity of the catalytic and cell wall binding domains is essential for its broad lytic activity. Virol Sin 30:45-51.

7.         Yang H, Zhang Y, Yu J, Huang Y, Zhang XE, Wei H_*. 2014. Novel chimeric lysin with high-level antimicrobial activity against methicillin-resistant Staphylococcus aureus in vitro and in vivo. Antimicrob Agents Chemother 58:536-542.

8.         Yang H, Zhang Y, Huang Y, Yu J, Wei H^*. 2014. Degradation of methicillin-resistant Staphylococcus aureus biofilms using a chimeric lysin. Biofouling 30:667-674.

9.         Yang H, Yu J, Wei H^*. 2014. Engineered bacteriophage lysins as novel anti-infectives. Front Microbiol 5:542.

10.      Yang H, Wang DB, Dong Q, Zhang Z, Cui Z, Deng J, Yu J, Zhang XE, Wei H^*. 2012. Existence of separate domains in lysin PlyG for recognizing Bacillus anthracis spores and vegetative cells. Antimicrob Agents Chemother 56:5031-5039.

11.       Yang H, He J^*, Hu F, Zheng C, Yu Z. 2010. Detection of Escherichia coli enoyl-ACP reductase using biarsenical-tetracysteine motif. Bioconjug Chem 21:1341-1348.

12.       杨航，余军平，危宏平^*. 2015. 裂解酶治疗的研究进展与应用前景. 微生物学通报 42:178-184.

13.       Zhang Y, Yang H, Yu J, Wei H. 2016. Rapid and sensitive detection of HIV-1 p24 antigen by immunomagnetic separation coupled with catalytic fluorescent immunoassay. Anal Bioanal Chem 408:6115-6121.

14.       Yu J, Zhang Y, Zhang Y, Li H, Yang H, Wei H. 2016. Sensitive and rapid detection of staphylococcus aureus in milk via cell binding domain of lysin. Biosens Bioelectron 77:366-371.

15.       Li H, Zhou LP, Luo J, Yu JP, Yang H, Wei HP. 2016. Rapid colorimetric pyrazinamide susceptibility testing of Mycobacterium tuberculosis. Int J Tuberc Lung Dis 20:462-467.

16.       Hu Y, Yang H, Wang J, Zhang Y, Yu J, Wei H. 2016. Comparison between a chimeric lysin ClyH and other enzymes for extracting DNA to detect methicillin resistant Staphylococcus aureus by quantitative PCR. World J Microbiol Biotechnol 32:1.

17.       Teng T, Yu J, Yang H, Wei H. 2015. Isolation and complete genome sequence of a novel virulent mycobacteriophage, CASbig. Virol Sin 30:76-79.

18.       Liu J, Zhang X, Yang H, Yuan J, Wei H, Yu J, Fang X. 2015. Study of the interactions between endolysin and bacterial peptidoglycan on S. aureus by dynamic force spectroscopy. Nanoscale 7:15245-15250.

19.       Hu W, Zhang Y, Yang H, Yu J, Wei H. 2015. Simple and rapid preparation of red fluorescence and red color S. aureus derived nanobioparticles for pathogen detection. J Microbiol Methods 115:47-53.

20.      Dong Q, Wang J, Yang H, Wei C, Yu J, Zhang Y, Huang Y, Zhang XE, Wei H. 2015. Construction of a chimeric lysin Ply187N-V12C with extended lytic activity against staphylococci and streptococci. Microb Biotechnol 8:210-220.

21.       Qiao J, Yu J, Yang H, Wei H. 2014. PCR-based in vitro synthesis of hepatitis C virus NS3 protease for rapid phenotypic resistance testing of protease inhibitors. J Clin Microbiol 52:1139-1145.

My research interests mainly focus on the discovery, engineering and application of novel antimicrobial therapeutics, i.e. bacteriophage endolysins, aimed to solve the antimicrobial resistant threats posed by highly pathogenic pathogens including bacillus, staphylococci, streptococci, pseudomonas, Acinetobacter, and enterococci. Novel endolysins were discovered by high through-put screening, in vitro evolution and direct modification methods as a effort to gain an extended host-range, intracellular killing capacity, or Gram-negative killing activity. We develop cell and animal models to evaluate the safety and efficacy of endolysins in therapeutic applications agaisnt biofilms, persisters and mixed infections. 

Another interest is the mechanisms of cross-talk between bacteiophage and the eukaryotic hosts, with a special emphasis on the modulation of phages on the reponses of eukaryotic cells to the infection of bacteria and viruses.

2019

1.       Invited speaker at the Cold Spring Harbor-Asia meeting: Bacterial Infection & Host Defense, Suzhou, China, 2019.04.08-2019.04.12, ‘Designing of chimeric lysins as non-traditional therapeutics against Streptococcal infections’

2018

1.       Invited speaker at the 2nd international lysin meeting, New York, USA, 2018.11.16-2018.11.18, ‘Designing and engineering of chimeric lysins specific for Streptococcus pneumoniae’ 

2014-2017

1.         Intervention of the cell-wall binding domain of a bacteriophage lysin in Staphylococcus aureus biofilm formation, poster, 2016, 1^st International Symposium on Anti-Bacterial Cell Wall Lytic Enzymes “The Lysin Meeting”, New York, the United States.

2.         ClyR: a chimeolysin with robust bacteriolytic activity and extended-spectrum streptococcal host range, poster, 2015, The 11^th Annual Meeting of Chinese Society for Virology & The 6^th Wuhan International Symposium on Modern Virology, Wuhan, China.

3.         Engineered bacteriophage lysins as novel anti-infectives, 2014, The 1^st Intenational Bacteriophage Meeting, Nanjing, China.

            4.    Chimeolysin as novel anti-infectives, 2014, The annual meeting of Microbiology of Hubei and Hunan provience, Hunan, China.

1. Daniel C. Nelson, University of Maryland, College Park；

2. Krystyna Dabrowska，Institute of Immunology and Experimental Therapy, Polish Academy of Sciences

已指导学生

李小红  硕士研究生  085238-生物工程  

王淑娟  硕士研究生  071005-微生物学  

周万里  硕士研究生  085238-生物工程  

王冠华  硕士研究生  085238-生物工程  

现指导学生

王奕尧  硕士研究生  071005-微生物学  

呼岳锋  硕士研究生  086000-生物与医药  

薛恒  博士研究生  071005-微生物学  

2018      National Natural Science Foundation of China, No. 31770192.

2018      Youth Innovation Promotion Association CAS

2016      WuHan Yellow Crane Talents (Science) Program.

2014      National Natural Science Foundation of China, No. 31400126.

                   2012      Post-Graduate Scientific and Technological Innovation Project of CAS