基本信息
徐平勇  男  博导  中国科学院生物物理研究所
电子邮件: pyxu@ibp.ac.cn
通信地址: 北京市朝阳区大屯路15号
邮政编码: 100101

研究领域

1 发展新型荧光蛋白和小分子探针及其相关的超高分辨显微成像新技术和方法

  超高分辨率显微成像技术能够突破衍射极限,提供生物大分子纳米尺度的定位和结构信息,是生物成像的研究热点和发展趋势,为解决生命科学领域的诸多问题提供了新的可能。该技术获得了2014年诺贝尔化学奖。该技术的实现及其在细胞生物学的应用需要具有特殊光化学性质的荧光蛋白和小分子探针。本课题组根据不同超高分辨成像技术的特点和需求,开发具有相应潜能的新型荧光探针,进一步推动超高分辨率显微成像的发展和应用。具体包括:开发了具有高亮度、完美单体性质和高标记密度的光转化荧光蛋白mEos3,反复光激活系列荧光蛋白mGeos系列,可应用于光激活定位显微镜(Photoactivated localization microscopy,PALM);具有高信噪比,高光稳定性的光开光荧光蛋白Skylan系列,例如可应用于非线性结构光照明显微镜(Nonlinerstructured-illumination microscopy,non-liner SIM)的Skylan-NS;光学波动超高分辨成像技术(Superresolution optical fluctuation imaging,SOFI)的Skylan-S;可逆饱和荧光转换显微镜(reversible saturable (or switchable) optical fluorescence transitions ,RESOLFT)的Skylan-R等。课题组还发展了具有自主知识产权的活细胞超高分辨显微成像方法SIMBA,具有时间和空间分辨率高、所需光学设备简单,可直接用于现有TIRFM、PALM/STORM等商用成像设备等优点。目前,课题组主要集中在发展可用于活细胞超高分辨显微成像技术的第二代荧光蛋白探针,可应用于光电联合显微成像技术的荧光探针等,并基于新型探针发展更高时空分辨率的活细胞超高分辨显微成像技术,并探讨它们在细胞生物学中的应用。

2 超高分辨率荧光显微成像新技术新方法在生物中的应用

当前在神经科学领域中应用超高分辨率方法研究蛋白的定位和相关功能研究尚处于早期阶段,表征神经元活性的探针和方法还有待于进一步的发展。本课题组将利用我们在荧光蛋白改造技术和方法上的优势,发展表征神经元活性的新型探针和方法,并在神经元中进行超高分辨成像定位和功能研究。

染色质动态结构变化与疾病发生密切相关,课题组的另一个重点关注的生物学科学问题是应用发展的超分辨成像探针、活细胞超分辨成像方法以及光电关联成像等方法研究染色质结构与基因表达调控。

教育经历

2000-09--2004-07   华中科技大学   博士
1996-09--2000-07   华中师范大学   硕士
1992-09--1996-07   华中师范大学   学士
学历
博士

学位
研究生

工作经历

   
工作简历
2014-09~现在, 中国科学院大学, 教授
2010-10~现在, 中国科学院生物物理研究所, 研究员
2006-09~2010-10,中国科学院生物物理研究所, 副研究员
2005-01~2007-04,中国科学院生物物理研究所, 博士后
2004-08~2006-09,中国科学院生物物理研究所, 助理研究员
社会兼职
2019-12-16-今,中国生物物理学会现代生物物理技术与方法分会专业委员会, 秘书长
2018-12-31-今,中国化学会生物物理化学分会专业委员会, 委员

教授课程

生物化学与分子生物学实验
科研实践I
分子生物学
医学成像技术
生物化学
细胞成像技术

专利与奖励

   
奖励信息
(1) 中国光学重要成果奖, , 其他, 2015
(2) 中科院青年创新促进会基金, , 院级, 2011
(3) 卢嘉锡青年人才奖, , 院级, 2009
(4) 国家自然科学奖, 二等奖, 国家级, 2008
专利成果
[1] 徐平勇, 徐涛, 张名姝, 付志飞, 彭鼎铭. 一种光控荧光蛋白. CN: CN112048003A, 2020-12-08.

[2] 徐平勇, 张法, 徐帆, 张名姝, 贺文婷. 一种贝叶斯显微成像方法. 中国: CN106023121B, 2018-08-31.

[3] 徐 涛, 徐平勇, 范俊梅, 姜 丽. 用于检测葡萄糖转运蛋白4上膜的探针和方法. 中国: CN101266242, 2008-09-17.

出版信息

   
发表论文
[1] Anyuan Liu, Xiaoshuai Huang, Wenting He, Fudong Xue, Yanrui Yang, Jiajia Liu, Liangyi Chen, Lin Yuan, Pingyong Xu. pHmScarlet is a pH-sensitive red fluorescent protein to monitor exocytosis docking and fusion steps. NATURE COMMUNICATIONS[J]. 2021, 12(1): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930027/.
[2] AlBari, Md Abdul Alim, Xu, Pingyong. Molecular regulation of autophagy machinery by mTOR-dependent and -independent pathways. ANNALS OF THE NEW YORK ACADEMY OF SCIENCESnull. 2020, 1467(1): 3-20, [3] Li, Hongjia, Xu, Fan, Gao, Shan, Zhang, Mingshu, Xue, Fudong, Xu, Pingyong, Zhang, Fa. Live-SIMBA: an ImageJ plug-in for the universal and accelerated single molecule-guided Bayesian localization super resolution microscopy (SIMBA) method. BIOMEDICAL OPTICS EXPRESS[J]. 2020, 11(10): 5842-5859, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7587271/.
[4] Zhang, Mingshu, Fu, Zhifei, Li, Changqing, Liu, Anyuan, Peng, Dingming, Xue, Fudong, He, Wenting, Gao, Shan, Xu, Fan, Xu, Dan, Yuan, Ling, Zhang, Fa, Xu, Zhiheng, Xu, Tao, Xu, Pingyong. Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein. NANO LETTERS[J]. 2020, 20(4): 2197-2208, https://www.webofscience.com/wos/woscc/full-record/WOS:000526413400001.
[5] Fu, Zhifei, Peng, Dingming, Zhang, Mingshu, Xue, Fudong, Zhang, Rui, He, Wenting, Xu, Tao, Xu, Pingyong. mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM. NATURE METHODS[J]. 2020, 17(1): 55-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000508582900034.
[6] Liu, Congyue, Liu, Wei, Wang, Shufeng, Li, Hongjia, Lv, Zhilong, Zhang, Fa, Zhang, Donghui, Teng, Junlin, Zheng, Tao, Li, Donghai, Zhang, Mingshu, Xu, Pingyong, Gong, Qihuang. Super-resolution nanoscopy by coherent control on nanoparticle emission. SCIENCE ADVANCES[J]. 2020, 6(16): https://www.webofscience.com/wos/woscc/full-record/WOS:000528276800002.
[7] Yuan, Lin, Liu, Qi, Wang, Zhe, Hou, Junjie, Xu, Pingyong. EI24 tethers endoplasmic reticulum and mitochondria to regulate autophagy flux (July, 2019). CELLULAR AND MOLECULAR LIFE SCIENCESnull. 2020, 77(11): 2255-2256, https://www.webofscience.com/wos/woscc/full-record/WOS:000497792200002.
[8] Yuan, Lin, Liu, Qi, Wang, Zhe, Hou, Junjie, Xu, Pingyong. EI24 tethers endoplasmic reticulum and mitochondria to regulate autophagy flux. CELLULAR AND MOLECULAR LIFE SCIENCES[J]. 2020, 77(8): 1591-1606, https://www.webofscience.com/wos/woscc/full-record/WOS:000526301600009.
[9] Luo Chen, Wang Huiyu, Liu Qi, He Wenting, Yuan Lin, Xu Pingyong. A genetically encoded ratiometric calcium sensor enables quantitative measurement of the local calcium microdomain in the endoplasmic reticulum. Biophysics Reports[J]. 2019, 5(1): 31-42, http://lib.cqvip.com/Qikan/Article/Detail?id=7001777564.
[10] 王哲, 徐平勇. 针对蛋白质转运的研究方法进展. 中国细胞生物学学报[J]. 2019, 25-32, http://lib.cqvip.com/Qikan/Article/Detail?id=88669090504849574849484854.
[11] Li, Zonghong, Zhou, Maoge, Cai, Zhaokui, Liu, Hongyang, Zhong, Wen, Hao, Qiang, Cheng, Dongwan, Hu, Xihao, Hou, Junjie, Xu, Pingyong, Xue, Yuanchao, Zhou, Yifa, Xu, Tao. RNA-binding protein DDX1 is responsible for fatty acid-mediated repression of insulin translation. NUCLEIC ACIDS RESEARCH[J]. 2018, 46(22): 12052-12066, https://www.webofscience.com/wos/woscc/full-record/WOS:000456714000035.
[12] 徐平勇. 一种基于定点标记与自组装荧光蛋白 mNeonGreen2用于精准、快速鉴定EI24蛋白拓 扑结构的方法. Progress in Biochemistry and Biophysics. 2018, [13] Yuan, Lin, Wang, Huiyu, Liu, Qi, Wang, Zhe, Zhang, Mingshu, Zhao, Yan, Liang, Kuo, Chen, Liangyi, Xu, Tao, Xu, Pingyong. Etoposide-induced protein 2.4 functions as a regulator of the calcium ATPase and protects pancreatic -cell survival. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2018, 293(26): 10128-10140, http://dx.doi.org/10.1074/jbc.RA118.002399.
[14] 徐平勇. LBI: deep learning guided Bayesian inference for structure reconstruction of super-resolution fluorescence microscopy. Bioinformatics. 2018, [15] Li, Yu, Xu, Fan, Zhang, Fa, Xu, Pingyong, Zhang, Mingshu, Fan, Ming, Li, Lihua, Gao, Xin, Han, Renmin. DLBI: deep learning guided Bayesian inference for structure reconstruction of super-resolution fluorescence microscopy. BIOINFORMATICS[J]. 2018, 34(13): 284-294, http://dx.doi.org/10.1093/bioinformatics/bty241.
[16] Fudong Xue, Wenting He, Fan Xu, Mingshu Zhang, Liangyi Chen, Pingyong Xu. Hessian single-molecule localization microscopy using sCMOS camera. 生物物理学报:英文版. 2018, 4(4): 215-221, http://lib.cqvip.com/Qikan/Article/Detail?id=676327039.
[17] Xue Fudong, He Wenting, Xu Fan, Zhang Mingshu, Chen Liangyi, Xu Pingyong. Hessian single-molecule localization microscopy using sCMOS camera. 生物物理学报:英文版[J]. 2018, 4(4): 215-221, http://lib.cqvip.com/Qikan/Article/Detail?id=676327039.
[18] 刘奇, 徐平勇, 袁琳. 一种利用荧光蛋白mNeonGreen2鉴定EI24蛋白拓扑结构的方法. 生物化学与生物物理进展. 2018, 45(12): 1280-1287, http://lib.cqvip.com/Qikan/Article/Detail?id=6100099180.
[19] Igarashi, Michihiro, Nozumi, Motohiro, Wu, LingGang, Zanacchi, Francesca Cella, Katona, Istvan, Barna, Laszlo, Xu, Pingyong, Zhang, Mingshu, Xue, Fudong, Boyden, Edward. New observations in neuroscience using superresolution microscopy. JOURNAL OF NEUROSCIENCE[J]. 2018, 38(44): 9459-9467, https://www.webofscience.com/wos/woscc/full-record/WOS:000448791600016.
[20] Liu Qi, Xu PingYong, Yuan Lin. A Method for Identifying The Topology of Etoposide-induced Protein 2.4 Using Split mNeonGreen2. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2018, 45(12): 1280-1287, https://www.webofscience.com/wos/woscc/full-record/WOS:000454568600010.
[21] Hou, Junjie, Li, Zonghong, Zhong, Wen, Hao, Qiang, Lei, Lei, Wang, Linlin, Zhao, Dongyu, Xu, Pingyong, Zhou, Yifa, Wang, You, Xu, Tao. Temporal Transcriptomic and Proteomic Landscapes of Deteriorating Pancreatic Islets in Type 2 Diabetic Rats. DIABETES[J]. 2017, 66(8): 2188-2200, https://www.webofscience.com/wos/woscc/full-record/WOS:000406014600016.
[22] Xu, Fan, Zhang, Mingshu, He, Wenting, Han, Renmin, Xue, Fudong, Liu, Zhiyong, Zhang, Fa, LippincottSchwartz, Jennifer, Xu, Pingyong. Live cell single molecule-guided Bayesian localization super resolution microscopy. CELL RESEARCHnull. 2017, 27(5): 713-716, https://www.webofscience.com/wos/woscc/full-record/WOS:000400443300014.
[23] Zhang, Di, Xu, XiaoLan, Li, Fei, Sun, HaiChen, Cui, YeQing, Liu, Shuang, Xu, PingYong. Upregulation of the checkpoint protein CHFR is associated with tumor suppression in pancreatic cancers. ONCOLOGY LETTERS[J]. 2017, 14(6): 8042-8050, https://www.webofscience.com/wos/woscc/full-record/WOS:000417293400237.
[24] Du, Wen, Zhou, Maoge, Zhao, Wei, Cheng, Dongwan, Wang, Lifen, Lu, Jingze, Song, Eli, Feng, Wei, Xue, Yanhong, Xu, Pingyong, Xu, Tao. HID-1 is required for homotypic fusion of immature secretory granules during maturation. ELIFE[J]. 2016, 5: 18134-18155, https://doaj.org/article/e6ece92f93ed466587d4b27667c15da4.
[25] Gao, Chongchong, Xu, XiaoLan, Li, Fei, Gong, Bengang, Liu, Shuang, Cui, Yeqing, Sun, Haichen, Xu, Pingyong, Zheng, Yamin, Jiang, Hua. Silencing pancreatic adenocarcinoma upregulated factor (PAUF) increases the sensitivity of pancreatic cancer cells to gemcitabine. TUMOR BIOLOGY[J]. 2016, 37(6): 7555-7564, [26] Zhang, Xi, Zhang, Mingshu, Li, Dong, He, Wenting, Peng, Jianxin, Betzig, Eric, Xu, Pingyong. Highly photostable, reversibly photoswitchable fluorescent protein with high contrast ratio for live-cell superresolution microscopy. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2016, 113(37): 10364-10369, http://www.corc.org.cn/handle/1471x/2376269.
[27] Zhang, Mingshu, Fu, Zhifei, Xu, Pingyong. Extending the spatiotemporal resolution of super-resolution microscopies using photomodulatable fluorescent proteins. JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCESnull. 2016, 9(3): http://www.corc.org.cn/handle/1471x/2374554.
[28] Tang, Juan, Zhang, Mingshu, Yin, HaoYan, Jing, Jing, Xie, Da, Xu, Pingyong, Zhang, JunLong. A photoactivatable Znsalen complex for super-resolution imaging of mitochondria in living cells. CHEMICAL COMMUNICATIONS[J]. 2016, 52(77): 11583-11586, http://www.corc.org.cn/handle/1471x/2375880.
[29] Li, Dong, Shao, Lin, Chen, BiChang, Zhang, Xi, Zhang, Mingshu, Moses, Brian, Milkie, Daniel E, Beach, Jordan R, Hammer, John A, III, Pasham, Mithun, Kirchhausen, Tomas, Baird, Michelle A, Davidson, Michael W, Xu, Pingyong, Betzig, Eric. Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics. SCIENCE[J]. 2015, 349(6251): https://www.webofscience.com/wos/woscc/full-record/WOS:000360646800044.
[30] Xu, Fan, Zhang, Mingshu, Liu, Zhiyong, Xu, Pingyong, Zhang, Fa. Bayesian localization microscopy based on intensity distribution of fluorophores. PROTEIN & CELL[J]. 2015, 6(3): 211-220, http://lib.cqvip.com/Qikan/Article/Detail?id=664275938.
[31] Ma, Haihao, Ma, Yuemin, Liu, Xuehui, Dyer, David H, Xu, Pingyong, Liu, Kaiyu, Lan, Que, Hong, Huazhu, Peng, Jianxin, Peng, Rong. NMR structure and function of Helicoverpa armigera sterol carrier protein-2, an important insecticidal target from the cotton bollworm. SCIENTIFIC REPORTS[J]. 2015, 5: https://www.webofscience.com/wos/woscc/full-record/WOS:000366135100001.
[32] Fan Xu, Mingshu Zhang, Zhiyong Liu, Pingyong Xu, Fa Zhang. Bayesian localization microscopy based on intensity distribution of fluorophores. 蛋白质与细胞:英文版. 2015, 211-220, http://lib.cqvip.com/Qikan/Article/Detail?id=664275938.
[33] Zhang, Xi, Chen, Xuanze, Zeng, Zhiping, Zhang, Mingshu, Sun, Yujie, Xi, Peng, Peng, Jianxin, Xu, Pingyong. Development of a Reversibly Switchable Fluorescent Protein for Super-Resolution Optical Fluctuation Imaging (SOFI). ACS NANO[J]. 2015, 9(3): 2659-2667, https://www.webofscience.com/wos/woscc/full-record/WOS:000351791800041.
[34] Zhang, Mingshu, Zhang, Xi, Yuan, Lin, Xu, Pingyong. RFP Tags for Labeling Secretory Pathway Proteins. BIOPHYSICAL JOURNALnull. 2015, 108(2): 322A-323A, https://www.webofscience.com/wos/woscc/full-record/WOS:000362849400032.
[35] 徐涛. Nanoscale Landscape of Phosphoinositides Revealed by Specific Pleckstrin Homology (PH) Domains Using Single-molecule Superresolution Imaging in the Plasma Membrane. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2015, 290(45): 26978-26993, http://dx.doi.org/10.1074/jbc.M115.663013.
[36] Zhang Xi, Zhang Mingshu, Xu Pingyong. C5-O-01Live cell superresolution imaging with unique photoactivatable fluorescent proteins. Microscopy[J]. 2015, [37] 赵新生, 高毅勤, 黄岩谊, 孙育杰, 葛颢, 王任小, 徐平勇, 尉志武. 生物物理化学研究进展. 化学通报[J]. 2014, 699-702, http://lib.cqvip.com/Qikan/Article/Detail?id=72888466504849524855484948.
[38] Zhang, Xi, Deng, Yongqiang, Chang, Hao, Ji, Chen, Zhang, Mingshu, Peng, Jianxin, Xu, Tao, Xu, Pingyong. The specific and rapid labeling of cell surface proteins with recombinant FKBP-fused fluorescent proteins. PROTEIN & CELLnull. 2014, 5(10): 800-803, http://lib.cqvip.com/Qikan/Article/Detail?id=663125380.
[39] Han, Liyang, Zhao, Yanhua, Zhang, Xi, Peng, Jianxin, Xu, Pingyong, Huan, Shuangyan, Zhang, Mingshu. RFP tags for labeling secretory pathway proteins. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2014, 447(3): 508-512, https://www.webofscience.com/wos/woscc/full-record/WOS:000336413900022.
[40] 徐平勇. beta-Lactonization of fluorinate porphyrin enhances LDL binding affinity, cellular uptake with selective intracellular localization. CHEMICAL SCIENCE. 2014, [41] Tang, Juan, Chen, JuanJuan, Jing, Jing, Chen, JiaZhen, Lv, Hongbin, Yu, Yi, Xu, Pingyong, Zhang, JunLong. beta-Lactonization of fluorinated porphyrin enhances LDL binding affinity, cellular uptake with selective intracellular localization. CHEMICAL SCIENCE[J]. 2014, 5(2): 558-566, https://www.webofscience.com/wos/woscc/full-record/WOS:000328954200015.
[42] Xu, Pingyong, Zhang, Xi, Zhang, Mingshu, Xu, Tao. Developing Photoactivatable Fluorescent Proteins for Diffraction-Limited and Superresolution Imaging. BIOPHYSICAL JOURNALnull. 2013, 104(2): 534A-534A, https://www.webofscience.com/wos/woscc/full-record/WOS:000316074305226.
[43] 徐涛. C13C4.5/Spinster,an evolutionarily conserved protein that regulates fertility in C-elegans through a lysosome-mediated lipid metabolism process. Protein Cell[J]. 2013, 4(5): 364-372, http://www.irgrid.ac.cn/handle/1471x/756499.
[44] Chen, JuanJuan, Jing, Jing, Chang, Hao, Rong, Yueguang, Hai, Yang, Tang, Juan, Zhang, JunLong, Xu, Pingyong. A sensitive and quantitative autolysosome probe for detecting autophagic activity in live and prestained fixed cells. AUTOPHAGY[J]. 2013, 9(6): 894-904, https://www.webofscience.com/wos/woscc/full-record/WOS:000323173900008.
[45] 徐涛. Light-induced protein translocation by genetically encoded unnatural amino acid in Caenorhabditis elegans. PROTEIN & CELLnull. 2013, 4(35): 883-886, https://www.webofscience.com/wos/woscc/full-record/WOS:000328450100001.
[46] Yu, Junwei, Zhang, Haining, Zhang, Mingshu, Deng, Yongqiang, Wang, Huiyu, Lu, Jingze, Xu, Tao, Xu, Pingyong. An aromatic amino acid in the coiled-coil 1 domain plays a crucial role in the auto-inhibitory mechanism of STIM1. BIOCHEMICAL JOURNAL[J]. 2013, 454: 401-409, https://www.webofscience.com/wos/woscc/full-record/WOS:000329975400004.
[47] 杨松, 滕岩, 徐平勇. 可用于双色双光子显微成像的新的荧光蛋白对. 生物化学与生物物理进展. 2012, 39(10): 1012-1016, http://lib.cqvip.com/Qikan/Article/Detail?id=43585319.
[48] Yang Song, Teng Yan, Xu Pingyong. 可用于双色双光子显微成像的新的荧光蛋白对. 生物化学与生物物理进展[J]. 2012, 39(10): 1012-1016, http://lib.cqvip.com/Qikan/Article/Detail?id=43585319.
[49] 徐平勇. A new series of reversibly switchable fluorescent proteins with beneficial properties for various applications. Proc Natl Acad Sci U S A. 2012, [50] Yang Song, Teng Yan, Xu PingYong. A Novel Fluorescent Protein Pair for Dual-color Two-photon Laser Scanning Microscopy. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2012, 39(10): 1012-1016, http://www.irgrid.ac.cn/handle/1471x/756703.
[51] Xu, Pingyong, Zhang, Mingshu, Chang, Hao, Zhang, Yongdeng, Ji, Wei, Xu, Tao. Novel Photoconvertible Fluorescent Proteins for Super-Resolution Imaging. BIOPHYSICAL JOURNALnull. 2012, 102(3): 723A-723A, https://www.webofscience.com/wos/woscc/full-record/WOS:000321561205128.
[52] 徐涛. Rational design of true monomeric and bright photoactivatable fluorescent proteins. Nature Methods[J]. 2012, 9(7): 727-U297, http://www.irgrid.ac.cn/handle/1471x/756906.
[53] Chang, Hao, Zhang, Mingshu, Ji, Wei, Chen, Juanjuan, Zhang, Yongdeng, Liu, Bei, Lu, Jingze, Zhang, Junlong, Xu, Pingyong, Xu, Tao. A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2012, 109(12): 4455-4460, http://dx.doi.org/10.1073/pnas.1113770109.
[54] Jing, Jing, Chen, JuanJuan, Hai, Yang, Zhan, Jinhui, Xu, Pingyong, Zhang, JunLong. Rational design of ZnSalen as a single and two photon activatable fluorophore in living cells. CHEMICAL SCIENCE[J]. 2012, 3(11): 3315-3320, http://dx.doi.org/10.1039/c2sc20764h.
[55] Li Zhengzheng, Liu Lin, Deng Yongqiang, Ji Wei, Du Wen, Xu Pingyong, Chen Liangyi, Xu Tao. Graded activation of CRAC channel by binding of different numbers of STIM1 to Orail subunits. 细胞研究:英文版[J]. 2011, 21(2): 305-315, http://lib.cqvip.com/Qikan/Article/Detail?id=37538305.
[56] 徐涛. HID-1 is a peripheral membrane protein primarily associated with the medial- and trans- Golgi apparatus. Protein Cell[J]. 2011, 2(1): 74-85, https://www.webofscience.com/wos/woscc/full-record/WOS:000310519400011.
[57] 徐涛. HID-1 is a novel player in the regulation of neuropeptide sorting. Biochemical Journal[J]. 2011, 434(3): 383-390, http://www.irgrid.ac.cn/handle/1471x/757379.
[58] Zhengzheng Li, Lin Liu, Yongqiang Deng, Wei Ji, Wen Du, Pingyong Xu, Liangyi Chen, Tao Xu. Graded activation of CRAC channel by binding of different numbers of STIM1 to Orail subunits. 细胞研究:英文版. 2011, 21(2): 305-315, http://lib.cqvip.com/Qikan/Article/Detail?id=37538305.
[59] Hai, Yang, Chen, JuanJuan, Zhao, Pei, Lv, Hongbin, Yu, Yi, Xu, Pingyong, Zhang, JunLong. Luminescent zinc salen complexes as single and two-photon fluorescence subcellular imaging probes. CHEMICAL COMMUNICATIONS[J]. 2011, 47(8): 2435-2437, http://www.irgrid.ac.cn/handle/1471x/757438.
[60] Chen, Yu, Wang, Yan, Ji, Wei, Xu, Pingyong, Xu, Tao. A Pre-Docking Role for Microtubules in Insulin-Stimulated Glucose Transporter 4 Translocation. BIOPHYSICAL JOURNALnull. 2010, 98(3): 670A-670A, https://www.webofscience.com/wos/woscc/full-record/WOS:000208762006369.
[61] Ji, Wei, Xu, Pingyong, Li, Zhengzheng, Lu, Jingze, Liu, Lin, Zhan, Yi, Chen, Yu, Hille, Bertil, Xu, Tao, Chen, Liangyi. Functional Stoichiometry Of The Unitary Calcium-release-activated Calcium Channel Revealed By Single-molecule Imaging. BIOPHYSICAL JOURNALnull. 2009, 96(3): 560A-560A, https://www.webofscience.com/wos/woscc/full-record/WOS:000426354000954.
[62] 徐涛. Mechanism of different spatial distributions of Caenorhabditis elegans and human STIM1 at resting state. Cell Calcium[J]. 2009, 45(1): 77-88, http://dx.doi.org/10.1016/j.ceca.2008.06.005.
[63] Lu, Jingze, He, Zixuan, Fan, Junmei, Xu, Pingyong, Chen, Liangyi. Overlapping functions of different dynamin isoforms in clathrin-dependent and -independent endocytosis in pancreatic beta cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2008, 371(2): 315-319, http://www.irgrid.ac.cn/handle/1471x/757725.
[64] 徐涛. A pre-docking role for microtubules in insulin-stimulated glucose transporter 4 translocation. FEBS Journal[J]. 2008, 275(4): 705-712, http://www.irgrid.ac.cn/handle/1471x/757750.
[65] 徐涛. Ca2+ triggers a novel clathrin-independent but actin-dependent fast endocytosis in pancreatic beta cells. TRAFFIC[J]. 2008, 9(6): 910-923, http://www.irgrid.ac.cn/handle/1471x/757732.
[66] Ji, Wei, Xu, Pingyong, Li, Zhengzheng, Lu, Jingze, Liu, Lin, Zhan, Yi, Chen, Yu, Hille, Bertil, Xu, Tao, Chen, Liangyi. Functional stoichiometry of the unitary calcium-release-activated calcium channel. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2008, 105(36): 13668-13673, http://www.irgrid.ac.cn/handle/1471x/757622.
[67] Dong, Yongming, Wan, Qunfang, Yang, Xiaofel, Bai, Li, Xu, Pingyong. Interaction of Munc18 and Syntaxin in the regulation of insulin secretion. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2007, 360(3): 609-614, https://www.webofscience.com/wos/woscc/full-record/WOS:000248345400016.
[68] Fan, Junmei, Yang, Xiaofei, Lu, Jingze, Chen, Liangyi, Xu, Pingyong. Role of H-abc domain in membrane trafficking and targeting of syntaxin 1A. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2007, 359(2): 245-250, https://www.webofscience.com/wos/woscc/full-record/WOS:000247494400010.
[69] Li, Zhengzheng, Lu, Jingze, Xu, Pingyong, Xie, Xiangyang, Chen, Liangyi, Xu, Tao. Mapping the interacting domains of STIM1 and Orai1 in Ca2+ release-activated Ca2+ channel activation. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2007, 282(40): 29448-29456, http://dx.doi.org/10.1074/jbc.M703573200.
[70] 徐涛. Dissecting multiple steps of GLUT4 trafficking and identifying the sites of insulin action. Cell Metabolism[J]. 2007, 5(1): 47-57, http://dx.doi.org/10.1016/j.cmet.2006.11.013.
[71] 徐涛. Aggregation of STIM1 underneath the plasma membrane induces clustering of Orai1. Biochemical and Biophysical Research Communications[J]. 2006, 350(4): 969-976, https://www.webofscience.com/wos/woscc/full-record/WOS:000241690800025.
[72] Yang, Xiaofei, Xu, Pingyong, Xiao, Yang, Xiong, Xiong, Xu, Tao. Domain requirement for the membrane trafficking and targeting of syntaxin 1A. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2006, 281(22): 15457-15463, http://dx.doi.org/10.1074/jbc.M513246200.
[73] 徐涛. Munc13-1 is required for the sustained release of insulin from pancreatic beta cells. Cell Metabolism[J]. 2006, 3(6): 463-468, https://www.webofscience.com/wos/woscc/full-record/WOS:000238199600010.
[74] Xu, PY, Bai, L, Tian, W, Xu, T. Direct interaction with syntaxin 1A defines the intracellular localization of Munc18a. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2005, 32(1): 31-36, https://www.webofscience.com/wos/woscc/full-record/WOS:000226433200006.
[75] 徐平勇, 白丽, 田伟, 徐涛. Syntaxin 1A与Munc18a在细胞内的相互作用和定位研究. 生物化学与生物物理进展. 2005, 32(1): 31-36, http://lib.cqvip.com/Qikan/Article/Detail?id=15253629.
[76] 徐涛. A new pair for inter- and intra-molecular FRET measurement. Biochemical and Biophysical Research Communications[J]. 2005, 330(3): 914-920, [77] 徐平勇, 李卉, 许想平. 含Syntaxin 1A基因的SFV表达载体的构建与表达. 华中科技大学学报:自然科学版[J]. 2005, 33(3): 108-111, http://lib.cqvip.com/Qikan/Article/Detail?id=15215277.
[78] 徐平勇, 徐涛. SM蛋白家族在分泌过程中的作用机制研究进展. 医学分子生物学杂志[J]. 2004, 1(2): 110-112, http://lib.cqvip.com/Qikan/Article/Detail?id=10231011.
[79] 杜雯, 徐平勇, 陈正望. 豌豆胰岛素基因克隆、表达及融合蛋白纯化. 华中科技大学学报:自然科学版[J]. 2004, 32(9): 102-104, http://lib.cqvip.com/Qikan/Article/Detail?id=10473706.
[80] 杨兴钰, 徐平勇. 5,5—二甲基—2—氧代—2—(N,N‘—对苯二胺基—二苄基)—二—1,3,2—氧磷杂环已烷的合成及表征. 合成化学. 2001, 9(6): 503-506, http://lib.cqvip.com/Qikan/Article/Detail?id=5798295.
[81] 杨兴钰, 徐平勇. N,N—对苯二胺基(2—羟基)二苄基膦酸四乙酯的合成及结构表征. 武汉大学学报:自然科学版. 2001, 47(2): 182-184, http://lib.cqvip.com/Qikan/Article/Detail?id=5177553.
[82] 徐平勇, 杨兴钰, 宦双燕. N,N-对苯二胺基(2-羟基)二苄基膦酸四乙酯的合成及结构表征. 武汉大学学报. 理学版[J]. 2001, 47(2): 182-, [83] 徐平勇, 宦双燕, 杨兴钰. 5,5-二甲基-2-氧代-2-(N,N-对苯二胺基-二苄基)-二-1,3,2-氧磷杂环己烷的合成及表征. 合成化学[J]. 2001, 9(6): 503-, 
发表著作
(1) 神经递质释放分子机制, Molecular Mechanisms of Neurotransmitter Release, Humana Press, 2008-09, 第 2 作者
(2) 光学纳米显微学和新型显微技术(章节:用于光学显微成像技术中的荧光蛋白), Optical Nanoscopy and Novel Microscopy Techniques (chapter: Fluorescent proteins for optical microscopy,pp 111-134), CRC Press, 2014-07, 第 1 作者

合作情况

        本课题组在超高分辨显微成像中的研究工作得到了该领域的广泛认可,许多国际国内著名的成像专家及其他领域专家都与申请人建立了紧密的合作关系。

项目协作单位

        HHMI,与2014 年诺贝尔化学奖获得者、超高分辨成像专家Eric Betzig合作,本课题组发展的新型荧光蛋白Skylan-NS 在非线性结构光(NL-SIM)超高分辨显微成像技术取得重大突破,该工作文章已于2015在Science上、2016年在PNAS上发表;
        NIH,与国际著名的美国科学院院士PALM 成像创始人Jennifer Lippincott-Schward合作,3B 成像方法上取得重大突破,成果已经在Cell Research上发表;
        国内与北京大学、中国科学院计算技术研究所等单位合作,并取得了一定成果。