081703-生物化工

生化分离工程，生物材料

2000-07--2005-12 北京化工大学 硕博连读
1996-07--2000-07 北京化工大学 本科

   

2009-07--今 中国科学院过程工程研究所 副研究员
2006-01--2009-07 中国科学院过程工程研究所 助理研究员

   

（1） 过程所科研和管理工作交流会青年科技创新优秀奖，研究所（学校）级，2014
（2） 过程所科研和管理工作交流会青年科技创新优秀奖，研究所（学校）级，2013
（3） 中国分析测试协会科学技术奖，一等奖，省级，2013
（4） 中国仪器仪表学会2011科技创新奖，省级，2011

[1] 李秀男, 夏宇飞. 一种微流控芯片及使用其制备脂质体纳米颗粒的方法. CN: CN116459884A, 2023-07-21.
[2] 李秀男, 陈芳宇, 陈超, 赵大伟, 张松平, 苏志国. 一种巧克力熔化温度的检测方法. CN: CN111999334B, 2021-12-28.
[3] 李秀男, 陈芳宇, 陈超, 赵大伟, 张松平, 苏志国. 一种温敏性水凝胶的凝胶化温度的测试方法. CN: CN111999333A, 2020-11-27.
[4] 李秀男, 陈超, 赵大伟, 宋艳滨, 闫雷, 苏志国. 一种带气泡陷阱的层析柱. CN: CN108970177B, 2020-04-14.
[5] 马光辉, 宋翠, 魏炜, 葛佳, 李秀男, 李强, 周炜清. 一种可移动的具有双梯度调节功能的流动相控制系统及其处理方法和用途. CN: CN108614033A, 2018-10-02.
[6] 马光辉, 宋翠, 魏炜, 葛佳, 李秀男, 李强, 周炜清. 一种可移动的具有双梯度调节功能的流动相控制系统. CN: CN208060444U, 2018-11-06.
[7] 苏志国, 马光辉, 赵岚, 李秀男, 张松平. 一种利用离子液体制备多糖微球的方法. CN: CN106496597A, 2017-03-15.
[8] 李秀男, 陈超, 赵大伟, 苏志国. 一种柱层析与膜过滤集成系统. CN: CN105688442A, 2016-06-22.
[9] 李秀男, 李亚琼, 陈超, 赵大伟, 苏志国. 一种应用低场核磁共振弛豫技术测定凝胶溶胀率的方法. CN: CN104764764A, 2015-07-08.
[10] 李秀男, 李亚琼, 陈超, 赵大伟, 苏志国. 一种应用低场核磁共振技术测定凝胶化时间的方法. CN: CN104764763A, 2015-07-08.
[11] 李秀男, 沈志刚, 苏志国, 崔万臣, 陈超. 一种荧光和紫外-可见吸收一体化流通池. CN: CN103698276A, 2014-04-02.
[12] 李秀男, 沈志刚, 苏志国, 崔万臣, 陈超. 一种车载便携式液相色谱仪. CN: CN103698428A, 2014-04-02.
[13] 李秀男, 沈志刚, 苏志国, 崔万臣. 生物技术药物高效纯化分析系统及其药物分离检测方法. CN: CN103293253A, 2013-09-11.
[14] 李秀男, 苏志国. 一种多参数一体化色谱流通池. CN: CN104062366A, 2014-09-24.
[15] 李秀男, 苏志国. 一种多功能的通用阀. CN: CN103133722A, 2013-06-05.
[16] 李秀男, 苏志国. 电极清洗、校正和保存装置及方法. CN: CN103257164A, 2013-08-21.
[17] 李秀男, 苏志国. 蛋白层析系统. CN: CN102190704A, 2011-09-21.
[18] 苏志国, 李秀男, 孙海虹, 马润宇. 一种分析与分离制备石杉碱甲和石杉碱乙的方法. CN: CN1704405A, 2005-12-07.
[19] 苏志国, 孙海虹, 李秀男. 一种从淫羊藿属植物中提取淫羊藿甙的方法. CN: CN1225470C, 2005-11-02.

   

[1] 赵雪, 陈超, 王文博, 赵大伟, 张松平, 张万忠, 袁洪水, 李秀男. 低场核磁共振分析乳酸菌细胞中水分分布及其变化. 食品与发酵工业[J]. 2023, 49(7): 133-139, http://lib.cqvip.com/Qikan/Article/Detail?id=7109404675.
[2] Zhang, Yao, Liu, Yongdong, Zhang, Bingyang, Yin, Shuang, Li, Xiunan, Zhao, Dawei, Wang, Weiying, Bi, Jingxiu, Su, Zhiguo. In vitro preparation of uniform and nucleic acid free hepatitis B core particles through an optimized disassembly-purification-reassembly process. PROTEIN EXPRESSION AND PURIFICATION[J]. 2021, 178: http://dx.doi.org/10.1016/j.pep.2020.105747.
[3] Chen, Fangyu, Chen, Chao, Zhao, Dawei, Zhang, Songping, Ma, Guanghui, Su, Zhiguo, Li, Xiunan. On-line monitoring of the sol-gel transition temperature of thermosensitive chitosan/beta-glycerophosphate hydrogels by low field NMR. CARBOHYDRATE POLYMERS[J]. 2020, 238: http://dx.doi.org/10.1016/j.carbpol.2020.116196.
[4] 王向娟, 李秀男, 陈超, 苏志国, 马光辉, 赵大伟, 余蓉. 核糖核酸酶A的错流超滤复性. 过程工程学报[J]. 2020, 20(12): 1455-1462, http://lib.cqvip.com/Qikan/Article/Detail?id=7103648280.
[5] Song, Yanmin, Yang, Yanli, Lin, Xuan, Li, Xiunan, Zhang, Xuan, Ma, Guanghui, Su, Zhiguo, Zhang, Songping. In-situ and sensitive stability study of emulsion and aluminum adjuvanted inactivated foot-and-mouth disease virus vaccine by differential scanning fluorimetry analysis. VACCINE[J]. 2020, 38(14): 2904-2912, http://dx.doi.org/10.1016/j.vaccine.2020.02.068.
[6] Fangyu Chen, Chao Chen, Dawei Zhao, Songping Zhang, Guanghui Ma, Zhiguo Su, Xiunan Li. On-line monitoring of the sol-gel transition temperature of thermosensitive chitosan/β-glycerophosphate hydrogels by low field NMR. CARBOHYDRATE POLYMERS. 2020, 238: http://dx.doi.org/10.1016/j.carbpol.2020.116196.
[7] Xiang, Jie, Zhang, Songping, Zhang, Guifeng, Li, Xiunan, Zhang, Chun, Luo, Jian, Yu, Rong, Su, Zhiguo. Recovery of human serum albumin by dual-mode chromatography from the waste stream of Cohn fraction V supernatant. JOURNAL OF CHROMATOGRAPHY A[J]. 2020, 1630: http://dx.doi.org/10.1016/j.chroma.2020.461451.
[8] 陈芳宇, 陈超, 赵大伟, 张松平, 马光辉, 苏志国, 李秀男. 低场核磁共振在线检测巧克力的熔化过程. 食品工业科技[J]. 2020, 41(23): 247-252,259, http://lib.cqvip.com/Qikan/Article/Detail?id=7103479763.
[9] 宋翠, 葛佳, 李秀男, 李强, 陈超, 王鹏, 马光辉. 一种可进行梯度调节的流动相控制系统在生物大分子表征中的应用. 过程工程学报[J]. 2017, 18(1): 171-176, http://ir.ipe.ac.cn/handle/122111/28763.
[10] Wang, Qi, Zhang, Chun, Liu, Liping, Li, Zenglan, Guo, Fangxia, Li, Xiunan, Luo, Jian, Zhao, Dawei, Liu, Yongdong, Su, Zhiguo. High hydrostatic pressure encapsulation of doxorubicin in ferritin nanocages with enhanced efficiency. JOURNAL OF BIOTECHNOLOGY[J]. 2017, 254: 34-42, http://dx.doi.org/10.1016/j.jbiotec.2017.05.025.
[11] Zhao, Lan, Zhu, Kai, Huang, Yongdong, Li, Qiang, Li, Xiunan, Zhang, Rongyue, Su, Zhiguo, Wang, Qibao, Ma, Guanghui. Enhanced binding by dextran-grafting to Protein A affinity chromatographic media. JOURNAL OF SEPARATION SCIENCE[J]. 2017, 40(7): 1493-1499, https://www.webofscience.com/wos/woscc/full-record/WOS:000399782900007.
[12] Chen, Chao, Li, Xiunan, Zhao, Dawei, Li, Yaqiong, Shi, Hong, Ma, Guanghui, Su, Zhiguo. Precise control of agarose media pore structure by regulating cooling rate. JOURNAL OF SEPARATION SCIENCE[J]. 2017, 40(22): 4467-4474, https://www.webofscience.com/wos/woscc/full-record/WOS:000416228000019.
[13] Li, Zenglan, Zhang, Chun, Zhang, Yan, Liu, Yongdong, Li, Xiunan, Ma, Guanghui, Luo, Jim, Su, Zhiguo. Prevention of aggregate formation through mechanism analysis in refolding of recombinant pertactin from Escherichia coli. PROCESS BIOCHEMISTRY[J]. 2017, 62: 80-90, http://dx.doi.org/10.1016/j.procbio.2017.07.001.
[14] Li, Yaqiong, Li, Xiunan, Chen, Chao, Zhao, Dawei, Su, Zhiguo, Ma, Guanghui, Yu, Rong. Sol-gel transition characterization of thermosensitive hydrogels based on water mobility variation provided by low field NMR. JOURNAL OF POLYMER RESEARCH[J]. 2017, 24(2): http://ir.ipe.ac.cn/handle/122111/22843.
[15] Wang, Qi, Liu, Yongdong, Zhang, Chun, Guo, Fangxia, Feng, Cui, Li, Xiunan, Shi, Hong, Su, Zhiguo. High hydrostatic pressure enables almost 100% refolding of recombinant human ciliary neurotrophic factor from inclusion bodies at high concentration. PROTEIN EXPRESSION AND PURIFICATION[J]. 2017, 133: 152-159, http://dx.doi.org/10.1016/j.pep.2017.03.014.
[16] Chen, Ying, Wang, Qi, Zhang, Chun, Li, Xiunan, Gao, Qiang, Dong, Changqing, Liu, Yongdong, Su, Zhiguo. Improving the refolding efficiency for proinsulin aspart inclusion body with optimized buffer compositions. PROTEIN EXPRESSION AND PURIFICATION[J]. 2016, 122(JUNE): 1-7, http://dx.doi.org/10.1016/j.pep.2016.01.015.
[17] Li, Yaqiong, Li, Xiunan, Chen, Chao, Zhao, Dawei, Su, Zhiguo, Ma, Guanghui, Yu, Rong. A rapid, non-invasive and non-destructive method for studying swelling behavior and microstructure variations of hydrogels. CARBOHYDRATE POLYMERS[J]. 2016, 151(OCT): 1251-1260, http://dx.doi.org/10.1016/j.carbpol.2016.06.054.
[18] Zhao Dawei, Liu Yongdong, Zhang Guifeng, Zhang Chun, Li Xiunan, Wang Qingqing, Shi Hong, Su Zhiguo. Interaction of arginine with protein during refolding process probed by amide H/D exchange mass spectrometry and isothermal titration calorimetry.. BIOCHIMICA ET BIOPHYSICA ACTA. 2015, http://kns.cnki.net/KCMS/detail/detail.aspx?QueryID=0&CurRec=1&recid=&FileName=SJPD2BA2867147F796DE2D822EEB3FCF0FF6&DbName=SJPD_05&DbCode=SJPD&yx=&pr=&URLID=&bsm=.
[19] Li, Xiunan, Li, Yaqiong, Chen, Chao, Zhao, Dawei, Wang, Xiaojun, Zhao, Lan, Shi, Hong, Ma, Guanghui, Su, Zhiguo. Pore size analysis from low field NMR spin-spin relaxation measurements of porous microspheres. JOURNAL OF POROUS MATERIALS[J]. 2015, 22(1): 11-20, http://www.irgrid.ac.cn/handle/1471x/944962.
[20] Dawei Zhao, Yongdong Liu, Guifeng Zhang, Chun Zhang, Xiunan Li, Qingqing Wang, Hong Shi, Zhiguo Su. Interaction of arginine with protein during refolding process probed by amide H/D exchange mass spectrometry and isothermal titration calorimetry. BBA - PROTEINS AND PROTEOMICS. 2015, 39-45, http://dx.doi.org/10.1016/j.bbapap.2014.10.007.
[21] 苏志国, 马光辉, 李秀男, 李岩, 刘永东, 张贵锋, 周炜清, 孔英俊, 赵岚, 孙李靖, 石红. 以保护活性为目标的生物技术药物层析分离纯化技术. 中国分析测试协会科学技术奖发展回顾. 2015, http://www.irgrid.ac.cn/handle/1471x/1188363.
[22] Zhao, Dawei, Liu, Yongdong, Zhang, Guifeng, Zhang, Chun, Li, Xiunan, Wang, Qingqing, Shi, Hong, Su, Zhiguo. Interaction of arginine with protein during refolding process probed by amide H/D exchange mass spectrometry and isothermal titration calorimetry. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS[J]. 2015, 1854(1): 39-45, http://dx.doi.org/10.1016/j.bbapap.2014.10.007.
[23] Yu, Mengran, Li, Yan, Zhang, Songping, Li, Xiunan, Yang, Yanli, Chen, Yi, Ma, Guanghui, Su, Zhiguo. Improving stability of virus-like particles by ion-exchange chromatographic supports with large pore size: Advantages of gigaporous media beyond enhanced binding capacity. JOURNAL OF CHROMATOGRAPHY A[J]. 2014, 1331(1): 69-79, http://dx.doi.org/10.1016/j.chroma.2014.01.027.
[24] Zhang, Chun, Liu, Yongdong, Zhao, Dawei, Li, Xiunan, Yu, Rong, Su, Zhiguo. Facile purification of Escherichia coli expressed tag-free recombinant human tumor necrosis factor alpha from supernatant. PROTEIN EXPRESSION AND PURIFICATION[J]. 2014, 95(1): 195-203, http://dx.doi.org/10.1016/j.pep.2013.12.012.
[25] Peng, Fei, Liu, Yongdong, Li, Xiunan, Sun, Lijing, Zhao, Dawei, Wang, Qingqing, Ma, Guanghui, Su, Zhiguo. PEGylation of G-CSF in organic solvent markedly increase the efficacy and reactivity through protein unfolding, hydrolysis inhibition and solvent effect. JOURNAL OF BIOTECHNOLOGY[J]. 2014, 170(1): 42-49, http://dx.doi.org/10.1016/j.jbiotec.2013.10.037.
[26] Zhao, Dawei, Liu, Yongdong, Wang, Yinjue, Li, Xiunan, Wang, Qingqing, Su, Zhiguo. Membrane combined with hydrophilic macromolecules enhances protein refolding at high concentration. PROCESS BIOCHEMISTRY[J]. 2014, 49(7): 1129-1134, http://dx.doi.org/10.1016/j.procbio.2014.03.010.
[27] Xiong, Zhidong, Zhou, Weiqing, Sun, Lijing, Li, Xiunan, Zhao, Dawei, Chen, Yi, Li, Juan, Ma, Guanghui, Su, Zhiguo. Konjac glucomannan microspheres for low-cost desalting of protein solution. CARBOHYDRATE POLYMERS[J]. 2014, 111(OCT): 56-62, http://dx.doi.org/10.1016/j.carbpol.2014.04.059.
[28] 王晓君, 李秀男, 李秀男, 马光辉, 苏志国. 低场核磁法测定凝胶过滤介质的孔径分布. 化工学报[J]. 2013, 64(11): 4090-4095, http://lib.cqvip.com/Qikan/Article/Detail?id=47547115.
[29] Zhao, Lan, Liu, Yongdong, Wang, Yinjue, Huang, Yongdong, Li, Xiunan, Li, Yan, Ma, Guanghui, Su, Zhiguo. Determination of leakage from antibody adsorbent: composition analysis and pH effect. BIOMEDICAL CHROMATOGRAPHY[J]. 2013, 27(9): 1089-1091, http://ir.ipe.ac.cn/handle/122111/13762.
[30] Ding, Fei, Li, XiuNan, Diao, JianXiong, Sun, Ye, Zhang, Li, Sun, Ying. Chiral recognition of metalaxyl enantiomers by human serum albumin: evidence from molecular modeling and photophysical approach. CHIRALITY[J]. 2012, 24(6): 471-480, http://dx.doi.org/10.1002/chir.22024.
[31] Ding, Fei, Li, XiuNan, Diao, JianXiong, Sun, Ye, Zhang, Li, Ma, Lin, Yang, XinLing, Zhang, Li, Sun, Ying. Potential toxicity and affinity of triphenylmethane dye malachite green to lysozyme. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY[J]. 2012, 78: 41-49, http://dx.doi.org/10.1016/j.ecoenv.2011.11.006.
[32] Ding, Fei, Zhang, Li, Diao, JianXiong, Li, XiuNan, Ma, Lin, Sun, Ying. Human serum albumin stability and toxicity of anthraquinone dye alizarin complexone: An albumin-dye model. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY[J]. 2012, 79: 238-246, http://dx.doi.org/10.1016/j.ecoenv.2012.01.009.
[33] Wang, Jun, Wang, Yinjue, Hu, Tao, Li, Xiunan, Huang, Yongdong, Liu, Yongdong, Ma, Guanghui, Su, Zhiguo. An oriented adsorption strategy for efficient solid phase PEGylation of recombinant staphylokinase by immobilized metal-ion affinity chromatography. PROCESS BIOCHEMISTRY[J]. 2012, 47(1): 106-112, http://dx.doi.org/10.1016/j.procbio.2011.10.016.
[34] Ding, Fei, Sun, Ye, Diao, JianXiong, Li, XiuNan, Yang, XinLing, Sun, Ying, Zhang, Li. Features of the complex of food additive hesperidin to hemoglobin. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY[J]. 2012, 106: 53-60, http://dx.doi.org/10.1016/j.jphotobiol.2011.10.004.
[35] Zhao, Lan, Liu, Yongdong, Huang, Yongdong, Li, Xiunan, Wang, Yinjue, Li, Yan, Ma, Guanghui, Su, Zhiguo. Deliberate manipulation of the surface hydrophobicity of an adsorbent for an efficient purification of a giant molecule with multiple subunits. JOURNAL OF SEPARATION SCIENCE[J]. 2011, 34(22): 3186-3193, http://www.irgrid.ac.cn/handle/1471x/735896.
[36] Kong, Yingjun, Zhang, Guifeng, Luo, Jian, Liu, Yongdong, Li, Xiunan, Ma, Guanghui, Zhao, Yalin, Yuan, Sanping, Su, Zhiguo. Facile purification and characterization of recombinant human antithrombin III from transgenic goat milk. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY[J]. 2011, 86(10): 1303-1309, http://www.irgrid.ac.cn/handle/1471x/735918.
[37] 郝素娟, 汪音爵, 康爱君, 刘永东, 李秀男, 石红, 马润宇, 马光辉, 苏志国. 非糖基化促红细胞生成素的聚乙二醇定点修饰及修饰产物性质. 高等学校化学学报[J]. 2010, 2239-2245, http://lib.cqvip.com/Qikan/Article/Detail?id=36243741.
[38] Hao SuJuan, Wang YinJue, Kang AiJun, Liu YongDong, Li XiuNan, Shi Hong, Ma RunYu, Ma GuangHui, Su ZhiGuo. Site-specific PEGylation of Recombinant Human Non-glycosylated Erythropoietin and Characterization of the Mono-PEGylated Conjugate. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE[J]. 2010, 31(11): 2239-2245, http://www.irgrid.ac.cn/handle/1471x/779186.
[39] Kong, Yingjun, Li, Xiunan, Bai, Gaoying, Ma, Guanghui, Su, Zhiguo. An automatic system for multidimensional integrated protein chromatography. JOURNAL OF CHROMATOGRAPHY A[J]. 2010, 1217(44): 6898-6904, http://dx.doi.org/10.1016/j.chroma.2010.08.067.
[40] Zhang, Dalei, Li, Xiunan, Hao, Dongxia, Li, Guisheng, Xu, Benming, Ma, Guanghui, Su, Zhiguo. Systematic purification of polydatin, resveratrol and anthraglycoside B from Polygonum cuspidatum Sieb. et Zucc.. SEPARATION AND PURIFICATION TECHNOLOGY[J]. 2009, 66(2): 329-339, http://dx.doi.org/10.1016/j.seppur.2008.12.013.
[41] Chen, Jing, Liu, Yongdong, Li, Xiunan, Wang, Yinjue, Ding, Hong, Ma, Guanghui, Su, Zhiguo. Cooperative effects of urea and L-arginine on protein refolding. PROTEIN EXPRESSION AND PURIFICATION[J]. 2009, 66(1): 82-90, http://dx.doi.org/10.1016/j.pep.2009.02.004.
[42] Yu, Pengzhan, Li, Xingqi, Li, Xiunan, Lu, Xiuling, Ma, Guanghui, Su, Zhiguo. Preparative purification of polyethylene glycol derivatives with polystyrene-divinylbenzene beads as chromatographic packing. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS[J]. 2007, 17(20): 5605-5609, http://dx.doi.org/10.1016/j.bmcl.2007.07.094.
[43] Li, XN, Ma, RY, Su, HH, Sun, HH, Ma, GH, Su, ZG, Zha, SH. Rapid purification of huperzine A and B with two polystyrene based resins by preparative low-pressure liquid chromatography. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES[J]. 2006, 29(4): 569-582, http://www.irgrid.ac.cn/handle/1471x/736315.
[44] Sun, HH, Li, XN, Ma, GH, Su, ZG. Polystyrene-type uniform porous microsphere enables high resolution and low-pressure chromatography of natural products - A case study with icariin purification. CHROMATOGRAPHIA[J]. 2005, 61(1-2): 9-15, http://www.irgrid.ac.cn/handle/1471x/736469.
[45] Li, XN, Sun, HH, Zhang, GF, Bi, JX, Ma, GH, Ma, RY, Su, ZG. A cyanopropyl reversed phase chromatography enables resolution of huperzine A and B on analytical and preparative scales. JOURNAL OF SEPARATION SCIENCE[J]. 2005, 28(17): 2363-2369, http://www.irgrid.ac.cn/handle/1471x/713017.
[46] 查圣华, 李秀男, 孙海虹, 林海, 苏志国. 从蛇足石杉中超声提取石杉碱甲和石杉碱乙. 天然产物研究与开发[J]. 2005, 17(1): 7-10, http://lib.cqvip.com/Qikan/Article/Detail?id=15010037.
[47] Sun, HH, Li, XN, Ma, GH, Su, ZG, Li, XN. A facile two-column chromatographic process for efficient purification of paclitaxel from crude extract. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES[J]. 2005, 28(4): 605-617, http://www.irgrid.ac.cn/handle/1471x/713018.
[48] 孙海虹, 李秀男, 苏志国. 耦合双柱层析分离提纯植物天然产物. 第一届全国化学工程与生物化工年会论文摘要集(下). 2004, 1, http://www.irgrid.ac.cn/handle/1471x/717308.

   

（1） 高效分离纯化装备研制及应用开发，主持，国家级，2014-01--2016-12
（2） 低场核磁共振分析蛋白质层析传质过程，主持，国家级，2014-01--2017-12
（3） 生物技术药物纯化分析仪器的开发和应用，主持，国家级，2013-10--2017-10
（4） 疫苗类生物颗粒分离纯化工作站的研发，参与，院级级，2012-11--2014-10
（5） 生物制造分离过程技术与装备，主持，国家级，2012-01--2015-12