干细胞命运调控与神经再生，神经类器官构建

   

0710J6-再生医学

神经生物学，神经疾病，干细胞治疗

2008-09--2011-07   山东大学   博士
2005-09--2008-07   山东大学   硕士
2001-09--2005-07   南京工业大学   学士

研究生

理学博士

   

2019-08~现在, 中国科学院动物研究所, 副研究员
2015-06~2019-08,中国科学院动物研究所, 助理研究员
2011-07~2015-06,中国科学院动物研究所, 博士后

   

（ 1 ） 一种通过血样检测判断动物体内存在移植物的方法, 发明专利, 2023, 第 4 作者, 专利号: CN116042848A

（ 2 ） 一种视网膜色素上皮细胞的扩增培养基及培养方法, 发明专利, 2023, 第 7 作者, 专利号: CN115873797A

（ 3 ） 一种神经细胞组合和其应用, 发明专利, 2023, 第 4 作者, 专利号: CN 115873796 A

（ 4 ） 一种神经细胞组合和其应用, 发明专利, 2023, 第 4 作者, 专利号: CN115873796A

（ 5 ） 用于指示中脑多巴胺神经前体细胞或神经元的报告系统, 发明专利, 2023, 第 5 作者, 专利号: CN 115558645 A

（ 6 ） 一种中脑多巴胺细胞群、其制造方法以及用途, 发明专利, 2022, 第 4 作者, 专利号: CN 115354028 A

（ 7 ） 一种中脑多巴胺细胞群、其制造方法以及用途, 发明专利, 2022, 第 4 作者, 专利号: CN115354028A

（ 8 ） 神经类器官及其制备方法, 发明专利, 2021, 第 5 作者, 专利号: CN113493768B

（ 9 ） 蛋白激酶抑制剂在抑制单倍体细胞二倍化中的用途, 专利授权, 2020, 第 4 作者, 专利号: CN108998410B

   

[1] Lin Feng, Da Li, Yao Tian, Chengshun Zhao, Yun Sun, Xiaolong Kou, Jun Wu, Liu Wang, Qi Gu, Wei Li, Jie Hao, Baoyang Hu, Yukai Wang. One-step cell biomanufacturing platform:porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo. 中国神经再生研究:英文版[J]. 2024, 第 13 作者  通讯作者  19(2): 458-464, http://lib.cqvip.com/Qikan/Article/Detail?id=7110261206.
[2] Tingting Gao, Xiyuan Zhao, Jie Hao, Yao Tian, Huike Ma, Wenjing Liu, Bin An, Faguo Sun, Shasha Liu, Baojie Guo, Shuaishuai Niu, Zhongwen Li, Chenxin Wang, Yukai Wang, Guihai Feng, Liu Wang, Wei Li, Jun Wu, Meijin Guo, Qi Zhou, Qi Gu. A scalable culture system incorporating microcarrier for specialised mesenchymal stem cells from human embryonic stem cells. MATERIALS TODAY BIO[J]. 2023, 第 14 作者20:100662: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196860/.
[3] YuKai Wang et al. Human midbrain dopaminergic progenitors. Cell Prolif[J]. 2023, https://onlinelibrary.wiley.com/doi/10.1111/cpr.13563.
[4] Baojie Guo et al. High-Strength Cell Sheets and Vigorous Hydrogels from Mesenchymal Stem Cells Derived from Human Embryonic Stem Cells. ACS Appl Mater Interfaces[J]. 2023, 
[5] YuKai Wang et al. Human neural stem cells. Cell Prolif[J]. 2023, https://onlinelibrary.wiley.com/doi/10.1111/cpr.13564.
[6] Wenliang Zhu, Mengqi Li, Jun Zou, Da Zhang, Minghui Fang, Yun Sun, Can Li, Mingming Tang, Yukai Wang, Qi Zhou, Tongbiao Zhao, Wei Li, Zheng Hu, Baoyang Hu. Induction of local immunosuppression in allogeneic cell transplantation by cell-type-specific expression of PD-L1 and CTLA4Ig. STEM CELL REPORTS[J]. 2023, 第 9 作者18(12): 2344-2355, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10724073/.
[7] Juan Yu, Nianye Yu, Yao Tian, Yifan Fang, Bin An, Guihai Feng, Jun Wu, Liu Wang, Jie Hao, Liqiang Wang, Qi Zhou, Wei Li, Yukai Wang, Baoyang Hu. Safety and efficacy of human ESC-derived corneal endothelial cells for corneal endothelial dysfunction. CELL & BIOSCIENCE[J]. 2023, 第 13 作者  通讯作者  13(1): 201, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629087/.
[8] Liang, Weizheng, He, Junli, Mao, Chenyu, Yu, Chengwei, Meng, Qingxue, Xue, Jun, Wu, Xueliang, Li, Shanliang, Wang, Yukai, Yi, Hongyang. Gene editing monkeys: Retrospect and outlook. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY. 2022, 第 9 作者  通讯作者  10: http://dx.doi.org/10.3389/fcell.2022.913996.
[9] Liang, Lingmin, Tian, Yao, Feng, Lin, Wang, Chaoqun, Feng, Guihai, Stacey, Glyn Nigel, ShyhChang, Ng, Wu, Jun, Hu, Baoyang, Li, Wei, Hao, Jie, Wang, Liu, Wang, Yukai. Single-cell transcriptomics reveals the cell fate transitions of human dopaminergic progenitors derived from hESCs. STEM CELL RESEARCH & THERAPY[J]. 2022, 第 13 作者  通讯作者  13(1): http://dx.doi.org/10.1186/s13287-022-03104-7.
[10] Zhao, Yilong, Wu, Jun, Li, Da, Liu, Jing, Chen, Weiqi, Hou, Zongren, Liu, Kailun, Jiang, Lingling, Chen, Xiaowei, Wang, Liu, Hu, Baoyang, Zong, Fangrong, Wang, Yukai, Wang, Yilong. Human ESC-derived immunity- and matrix- regulatory cells ameliorated white matter damage and vascular cognitive impairment in rats subjected to chronic cerebral hypoperfusion. CELL PROLIFERATION[J]. 2022, 第 13 作者  通讯作者  55(5): http://dx.doi.org/10.1111/cpr.13223.
[11] Sun, Yun, Feng, Lin, Liang, Lingmin, Stacey, Glyn N, Wang, Chaoqun, Wang, Yukai, Hu, Baoyang. Neuronal cell-based medicines from pluripotent stem cells: Development, production, and preclinical assessment. STEM CELLS TRANSLATIONAL MEDICINE[J]. 2021, 第 6 作者  通讯作者  10(Suppl 2): S31-S40, http://dx.doi.org/10.1002/sctm.20-0522.
[12] Zhang, Qi, Wang, Yanlin, Wang, Yukai, Liu, Han, Sun, Huifang, Wang, Zhuoya, Shi, Changhe, Yang, Jing, Xu, Yuming. Autologous Transplantation for Parkinson's Disease Patients: Feasibility and Challenge. NEUROSCIENCE BULLETIN[J]. 2021, 第 3 作者37(7): 1085-1086, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=7004167&detailType=1.
[13] Liu, Jing, Hou, Zongren, Wu, Jun, Liu, Kailun, Li, Da, Gao, Tingting, Liu, Wenjing, An, Bin, Sun, Yun, Mo, Fan, Wang, Liu, Wang, Yukai, Hao, Jie, Hu, Baoyang. Infusion of hESC derived Immunity-and-matrix regulatory cells improves cognitive ability in early-stage AD mice. CELL PROLIFERATION[J]. 2021, 第 12 作者  通讯作者  54(8): http://dx.doi.org/10.1111/cpr.13085.
[14] Wu, Jun, Song, Dingyun, Li, Zhongwen, Guo, Baojie, Xiao, Yani, Liu, Wenjing, Liang, Lingmin, Feng, Chunjing, Gao, Tingting, Chen, Yanxia, Li, Ying, Wang, Zai, Wen, Jianyan, Yang, Shengnan, Liu, Peipei, Wang, Lei, Wang, Yukai, Peng, Liang, Stacey, Glyn Nigel, Hu, Zheng, Feng, Guihai, Li, Wei, Huo, Yan, Jin, Ronghua, ShyhChang, Ng, Zhou, Qi, Wang, Liu, Hu, Baoyang, Dai, Huaping, Hao, Jie. Immunity-and-matrix-regulatory cells derived from human embryonic stem cells safely and effectively treat mouse lung injury and fibrosis. CELL RESEARCH[J]. 2020, 第 17 作者30(9): 794-809, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=6805741&detailType=1.
[15] Tan, Yuanqing, Wang, Lei, Chen, Gang, Liu, Wenjing, Li, Zhongwen, Wang, Yukai, Wang, Liu, Li, Wei, Wu, Jun, Hao, Jie. Hyaluronate supports hESC-cardiomyocyte cell therapy for cardiac regeneration after acute myocardial infarction. CELL PROLIFERATION[J]. 2020, 第 6 作者53(12): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705924/.
[16] Li, Zhongwen, Niu, Shuaishuai, Guo, Baojie, Gao, Tingting, Wang, Lei, Wang, Yukai, Wang, Liu, Tan, Yuanqing, Wu, Jun, Hao, Jie. Stem cell therapy for COVID-19, ARDS and pulmonary fibrosis. CELL PROLIFERATION. 2020, 第 6 作者53(12): http://dx.doi.org/10.1111/cpr.12939.
[17] Li, Zhongwen, Wu, Jun, Wang, Lei, Han, Weifang, Yu, Juan, Liu, Xin, Wang, Yukai, Zhang, Ying, Feng, Guihai, Li, Wei, Stacey, Glyn Niger, Gu, Qi, Hu, Baoyang, Wang, Liu, Zhou, Qi, Hao, Jie. Generation of qualified clinical-grade functional hepatocytes from human embryonic stem cells in chemically defined conditions. CELLDEATHDISEASE[J]. 2019, 第 7 作者10(10): http://dx.doi.org/10.1038/s41419-019-1967-5.
[18] Wang, Jiaqiang, Wang, Leyun, Feng, Guihai, Wang, Yukai, Li, Yufei, Li, Xin, Liu, Chao, Jiao, Guanyi, Huang, Cheng, Shi, Junchao, Zhou, Tong, Chen, Qi, Liu, Zhonghua, Li, Wei, Zhou, Qi. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. CELL[J]. 2018, 第 4 作者175(7): 1887-1901.e18, http://dx.doi.org/10.1016/j.cell.2018.11.039.
[19] 吴骏, 王昱凯, 王磊, 李仲文, 梁灵敏, 冯琳, 郭保杰, 于娟, 王柳, 郝捷, 周琪. 人胚胎干细胞的临床转化研究进展. 中国细胞生物学学报. 2018, 第 2 作者2116-2128, https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFDAUTO&filename=XBZZ2018S1003&v=MTE5MjBGckNVUjdxZmJ1UnVGeUhrVUwvQlBTL1JkTEc0SDltdnJvOUZaNFI4ZVgxTHV4WVM3RGgxVDNxVHJXTTE=.
[20] Li, Xia, Ma, Ruoyu, Gu, Qi, Liang, Lingmin, Wang, Lei, Zhang, Ying, Wang, Xianning, Liu, Xin, Li, Zhongwen, Fang, Jinhui, Wu, Jun, Wang, Yukai, Li, Wei, Hu, Baoyang, Wang, Liu, Zhou, Qi, Hao, Jie. A fully defined static suspension culture system for large-scale human embryonic stem cell production. CELL DEATH & DISEASE[J]. 2018, 第 12 作者9: https://doaj.org/article/517fbb632f3142c385073e2f1824afd8.
[21] Wang, YuKai, Zhu, WanWan, Wu, MengHua, Wu, YiHui, Liu, ZhengXin, Liang, LingMin, Sheng, Chao, Hao, Jie, Wang, Liu, Li, Wei, Zhou, Qi, Hu, BaoYang. Human Clinical-Grade Parthenogenetic ESC-Derived Dopaminergic Neurons Recover Locomotive Defects of Nonhuman Primate Models of Parkinson's Disease. STEM CELL REPORTS[J]. 2018, 第 1 作者11(1): 171-182, http://dx.doi.org/10.1016/j.stemcr.2018.05.010.
[22] Wang, Xuepeng, Li, Tianda, Cui, Tongtong, Yu, Dawei, Liu, Chao, Jiang, Liyuan, Feng, Guihai, Wang, Lei, Fu, Rui, Zhang, Xinxin, Hao, Jie, Wang, Yukai, Wang, Liu, Zhou, Qi, Li, Wei, Hu, Baoyang. Human embryonic stem cells contribute to embryonic and extraembryonic lineages in mouse embryos upon inhibition of apoptosis. CELL RESEARCH[J]. 2018, 第 12 作者28(1): 126-129, https://www.webofscience.com/wos/woscc/full-record/WOS:000423834700012.
[23] He, ZhengQuan, Xia, BaoLong, Wang, YuKai, Li, Jing, Feng, GuiHai, Zhang, LinLin, Li, YuHuan, Wan, HaiFeng, Li, TianDa, Xu, Kai, Yuan, XueWei, Li, YuFei, Zhang, XinXin, Zhang, Ying, Wang, Liu, Li, Wei, Zhou, Qi. Generation of Mouse Haploid Somatic Cells by Small Molecules for Genome-wide Genetic Screening. CELL REPORTS[J]. 2017, 第 3 作者20(9): 2227-2237, http://dx.doi.org/10.1016/j.celrep.2017.07.081.
[24] Lu, Xiaoyin, Wang, Yukai, Yan, Long, Wang, Libin, Li, Wei, Wang, Hongmei. Variations in mesenchymal-epithelial transition-related transcription factors during reprogramming of somatic cells from different germ layers into iPSCs. JOURNAL OF GENETICS AND GENOMICS[J]. 2016, 第 2 作者43(10): 609-612, http://dx.doi.org/10.1016/j.jgg.2016.04.001.
[25] 王昱凯, 周琪, 胡宝洋. 人胚胎干细胞来源多巴胺前体细胞治疗帕金森病猴. 生命科学[J]. 2016, 第 1 作者28(8): 902-906, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=5791210&detailType=1.
[26] Li, Xin, Cui, Xiaolong, Wang, JiaQiang, Wang, YuKai, Li, YuFei, Li, Wei, Wan, HaiFeng, Li, TianDa, Feng, GuiHai, Shuai, Ling, Li, ZhiKun, Gu, Qi, Hao, Jie, Wang, Liu, Zhao, XiaoYang, Liu, ZhongHua, Wang, XiuJie, Li, Wei, Zhou, Qi. Generation and application of mouse-rat allodiploid embryonic stem cells. CELL[J]. 2016, 第 4 作者164(1-2): 279-292, http://dx.doi.org/10.1016/j.cell.2015.11.035.
[27] Shuai, Ling, Wang, YuKai, Dong, MingZhu, Wang, XuePeng, Sang, LiSi, Wang, Mei, Wan, HaiFeng, Luo, GuanZheng, Gu, TianTian, Yuan, Yan, Feng, ChunJing, Teng, Fei, Li, Wei, Liu, XiuYing, Li, TianDa, Wang, Liu, Wang, XiuJie, Zhao, XiaoYang, Zhou, Qi. Durable pluripotency and haploidy in epiblast stem cells derived from haploid embryonic stem cells in vitro. JOURNAL OF MOLECULAR CELL BIOLOGY[J]. 2015, 第 2 作者7(4): 326-337, https://www.webofscience.com/wos/woscc/full-record/WOS:000359665200005.
[28] Li, Zhikun, Wang, Libin, Wang, Yukai, Liu, Lei, Wang, Liu, Li, Wei, Zhou, Qi. Generation of an LncRNA Gtl2-GFP Reporter for Rapid Assessment of Pluripotency in Mouse Induced Pluripotent Stem Cells. JOURNAL OF GENETICS AND GENOMICS[J]. 2015, 第 3 作者42(3): 125-128, http://dx.doi.org/10.1016/j.jgg.2015.02.004.
[29] Wang, Libin, Cao, Jiani, Wang, Yukai, Lan, Tianshu, Liu, Lei, Wang, Weixu, Jin, Ning, Gong, Jiaqi, Zhang, Chao, Teng, Fei, Yan, Guoliang, Li, Chun, Li, Jiali, Wan, Haifeng, Hu, Baoyang, Li, Wei, Zhao, Xiaoyang, Qi, Zhongquan, Zhao, Tongbiao, Zhou, Qi. Immunogenicity and functional evaluation of iPSC-derived organs for transplantation. CELL DISCOVERY[J]. 2015, 第 3 作者1: 15015, https://www.webofscience.com/wos/woscc/full-record/WOS:000414776800015.
[30] 王昱凯, 周琪. 细胞重编程改写细胞命运：细胞的返老还童——2012年诺贝尔生理学或医学奖简介. 自然杂志[J]. 2012, 第 1 作者34(6): 327-331, http://lib.cqvip.com/Qikan/Article/Detail?id=44162463.

   

（ 1 ） 人中脑多巴胺神经细胞分化的阶段性命运调控研究, 负责人, 国家任务, 2020-01--2023-12
（ 2 ） 单基因遗传病的基因治疗研究, 参与, 国家任务, 2019-10--2023-12
（ 3 ） 类生命机器人智能, 参与, 中国科学院计划, 2022-07--2027-07
（ 4 ） 神经和肝脏疾病的细胞治疗, 负责人, 地方任务, 2023-10--2027-12