新型颗粒制剂研制及其在生物医药领域的应用研究

2003-09--2007-06   中国科学院过程工程研究所   博士
2000-09--2003-04   哈尔滨工程大学   硕士
1996-09--2000-07   哈尔滨工程大学   学士

2018-09~现在, 中国科学院过程工程研究所, 研究员
2010-06~2018-09,中国科学院过程工程研究所, 副研究员
2007-07~2010-05,中国科学院过程工程研究所, 助理研究员

   

（1） 中国颗粒学会青年颗粒学奖, , 其他, 2018
（2） Biomaterials2006-2010引用前五十位（中国大陆作者）, , 其他, 2010
（3） Int. J. Pharm. 2006年度原创研究最高引用奖, , 其他, 2006

[1] 马光辉, 吴颉, 吴楠, 张晴. 一种纳微气泡及其制备方法和应用. 202110615243.6, 2021-06-02.

[2] 马光辉, 岳华, 吴颉, 吴楠, 苗春宇, 周炜清. 高分子凝胶颗粒、其制备方法、包含其的复合凝胶颗粒及用途. CN: CN107855080B, 2020-09-08.

[3] 马光辉, 吴颉, 邹勇娟, 吴楠, 苗春宇, 周炜清. 一种水包油包凝胶乳液及其制备方法和应用. CN: CN109464395A, 2019-03-15.

[4] 马光辉, 吴颉, 吴楠, 苗春宇, 周炜清. 一种抗原热稳定乳液及其制备方法和应用. CN: CN109364243A, 2019-02-22.

[5] 马光辉, 吴颉, 夏宇飞, 苗春宇, 杜逸群, 周炜清. 一种硬乳液纳微球及其制备方法和应用. 中国: CN108403659A, 2018.08.17.

[6] 马光辉, 吴颉, 范清泽, 苗春宇, 巩方玲. 一种粒径均一的非球状聚合物颗粒及其制备方法和用途. 中国: CN105832704B, 2018-09-18.

[7] 马光辉, 吴颉, 夏宇飞, 苗春宇, 杜逸群, 周炜清. 一种颗粒型佐剂及其制备方法和应用. 中国: CN108324938A, 2018-07-27.

[8] 马光辉, 吴颉, 杨柳青, 夏宇飞, 齐峰, 范清泽. 一种不含表面活性剂的水包油乳液及其用途. 中国: CN104013955A, 2014-09-03.

[9] 马光辉, 齐峰, 吴颉, 周炜清. 一种粒径均一的Pickering乳液、制备方法及其应用. 中国: CN104001437A, 2014-08-27.

[10] 马光辉, 赵希, 吴颉, 崔金梅, 周炜清, 苏志国. 一种高强度多糖类凝胶微球、制备方法及其用途. 中国: CN103769057A, 2014-05-07.

[11] 马光辉, 吴颉, 赵希, 苏志国, 崔金梅, 周炜清. 一种双孔多糖微球及其制备方法、用途. 中国: CN103341172A, 2013-10-09.

[12] 马光辉, 赵希, 吴颉, 苏志国, 崔金梅, 周炜清. 一种大孔琼脂糖微球及其制备方法. 中国: CN103055773A, 2013-04-24.

[13] 马光辉, 赵希, 吴颉, 巩方玲, 周炜清, 苏志国. 一种分级式控温型膜乳化器. 中国: CN103007793A, 2013-04-03.

[14] 马光辉, 吴颉, 吴有斌, 苏志国, 王月琦. 一种自固化微球及其制备方法和应用. 中国: CN102138904A, 2011-08-03.

[15] 马光辉, 苏志国, 吴颉. 一种可注射型pH敏感壳聚糖季铵盐水凝胶及其制备方法. 中国: CN1830420, 2006-09-13.

   

[1] Zou, Yongjuan, Liu, Xiaoxuan, Chen, Qiuling, Oku, Hiroyuki, Ma, Guanghui, Wu, Jie. Acid-Responsive Immune-Enhancing Chitosan Formulation Capable of Transforming from Particle Stabilization to Polymer Chain Stabilization. ACS APPLIED MATERIALS & INTERFACES. 2023, http://dx.doi.org/10.1021/acsami.2c17505.
[2] Wu, Nan, Chen, Qiuting, Zou, Yongjuan, Miao, Chunyu, Ma, Guanghui, Wu, Jie. Chitosan particle-emulsion complex adjuvants: The effect of particle distribution on the immune intensity and response type. CARBOHYDRATE POLYMERS[J]. 2023, 309: http://dx.doi.org/10.1016/j.carbpol.2023.120673.
[3] Yanan Li, Jie Wu, Hiroyuki Oku, Guanghui Ma. Polymer‐Modified Micromotors with Biomedical Applications: Promotion of Functionalization. ADVANCED NANOBIOMED RESEARCH[J]. 2022, 2(10): n/a-n/a, https://doaj.org/article/068242c65ba048cea69b312c70e52fda.
[4] Wang, Danyang, Zou, Yongjuan, Wang, Ning, Wu, Jie. Chitosan hydrochloride salt stabilized emulsion as vaccine adjuvant. CARBOHYDRATE POLYMERS[J]. 2022, 296: http://dx.doi.org/10.1016/j.carbpol.2022.119879.
[5] Lin, Xuan, Sheng, Yanan, Zhang, Xuan, Li, Zhengjun, Yang, Yanli, Wu, Jie, Su, Zhiguo, Ma, Guanghui, Zhang, Songping. Oil-in-ionic liquid nanoemulsion-based intranasal delivery system for influenza split-virus vaccine. JOURNAL OF CONTROLLED RELEASE[J]. 2022, 346: 380-391, http://dx.doi.org/10.1016/j.jconrel.2022.04.036.
[6] 胡微, 吴颉, 中西秀树. 形貌调控与镀层修饰结合制备口服疫苗载体. 中国生物工程杂志[J]. 2022, 42(8): 40-51, http://lib.cqvip.com/Qikan/Article/Detail?id=7108150468.
[7] Zhang, Tong, Liu, Fuguo, Wu, Jie, Ngai, To. Pickering emulsions stabilized by biocompatible particles: A review of preparation, bioapplication, and perspective. PARTICUOLOGYnull. 2022, 64(5): 110-120, http://dx.doi.org/10.1016/j.partic.2021.07.003.
[8] Du, Yiqun, Song, Tiantian, Wu, Jie, Gao, XiaoDong, Ma, Guanghui, Liu, Yuchen, Xia, Yufei. Engineering mannosylated pickering emulsions for the targeted delivery of multicomponent vaccines. BIOMATERIALS[J]. 2022, 280: http://dx.doi.org/10.1016/j.biomaterials.2021.121313.
[9] Fan, Qingze, Miao, Chunyu, Huang, Yilan, Yue, Hua, Wu, Anguo, Wu, Jianming, Wu, Jie, Ma, Guanghui. Hydroxypropyltrimethyl ammonium chloride chitosan-based hydrogel as the split H5N1 mucosal adjuvant: Structure-activity relationship. CARBOHYDRATE POLYMERS[J]. 2021, 266: http://dx.doi.org/10.1016/j.carbpol.2021.118139.
[10] Zonghui Zuo, Yongjuan Zou, Qiang Li, Yongxia Guo, Tianyuan Zhang, Jie Wu, Cheng He, Francis O Eko. Intranasal immunization with inactivated chlamydial elementary bodies formulated in VCG-chitosan nanoparticles induces robust immunity against intranasal Chlamydia psittaci challenge. SCIENTIFIC REPORTS[J]. 2021, 11(1): 1-17, https://doaj.org/article/8fd97ec6e19a4235a29c2fa8339311db.
[11] Chen, Qiuting, Wu, Nan, Gao, Yuhui, Wang, Xiaojun, Wu, Jie, Ma, Guanghui. Alum Pickering Emulsion as Effective Adjuvant to Improve Malaria Vaccine Efficacy. VACCINES[J]. 2021, 9(11): http://dx.doi.org/10.3390/vaccines9111244.
[12] 张晴, 吴颉, 马光辉. 用于超声造影成像的颗粒稳定气泡制备. 过程工程学报[J]. 2021, [13] Wang, Jianghua, Wang, Shuang, Ye, Tong, Li, Feng, Gao, Xiaoyong, Wang, Yan, Ye, Peng, Qing, Shuang, Wang, Changlong, Yue, Hua, Wu, Jie, Wei, Wei, Ma, Guanghui. Choice of Nanovaccine Delivery Mode Has Profound Impacts on the Intralymph Node Spatiotemporal Distribution and Immunotherapy Efficacy. ADVANCED SCIENCE[J]. 2020, 7(19): https://doaj.org/article/eb7b525e7575405c8b46079997716871.
[14] Zou, Yongjuan, Li, Shuai, Ngai, To, Zhang, Songping, Ma, Guanghui, Wu, Jie. Green preparation of hydrogel particles-in-emulsions for simultaneous enhancement of humoral and cell-mediated immunity. ENGINEERING IN LIFE SCIENCES[J]. 2020, 20(11): 514-524, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7645649/.
[15] Xieru Bao, Jie Wu, Guanghui Ma. Sprayed Pickering emulsion with high antibacterial activity for wound healing. PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL[J]. 2020, 30(5): 669-676, http://dx.doi.org/10.1016/j.pnsc.2020.08.001.
[16] Wu, Jie, Ma, Guanghui. Biomimic strategies for modulating the interaction between particle adjuvants and antigen-presenting cells. BIOMATERIALS SCIENCE[J]. 2020, 8(9): 2366-2375, https://www.webofscience.com/wos/woscc/full-record/WOS:000532529400002.
[17] Zou, Yongjuan, Wu, Nan, Miao, Chunyu, Yue, Hua, Wu, Jie, Ma, Guanghui. A novel multiple emulsion enhanced immunity via its biomimetic delivery approach. JOURNAL OF MATERIALS CHEMISTRY B[J]. 2020, 8(33): 7365-7374, https://www.webofscience.com/wos/woscc/full-record/WOS:000563148600003.
[18] Feng Qi, Jie Wu, Hao Li, Guanghui Ma. Recent research and development of PLGA/PLA microspheres/nanoparticles: A review in scientific and industrial aspects. 化学科学与工程前沿:英文版[J]. 2019, 13(1): 14-27, http://lib.cqvip.com/Qikan/Article/Detail?id=7001649010.
[19] Du, Yiqun, Xia, Yufei, Zou, Yongjuan, Hu, Yuning, Fu, Jiaqi, Wu, Jie, Gao, XiaoDong, Ma, Guanghui. Exploiting the Lymph-Node-Amplifying Effect for Potent Systemic and Gastrointestinal Immune Responses via Polymer/Lipid Nanoparticles. ACS NANO[J]. 2019, 13(12): 13809-13817, https://www.webofscience.com/wos/woscc/full-record/WOS:000505633300021.
[20] Zhao, Xi, Huang, Lan, Wu, Jie, Huang, YongDong, Zhao, Lan, Wu, Nan, Zhou, WeiQing, Hao, DongXia, Ma, GuangHui, Su, ZhiGuo. Fabrication of rigid and macroporous agarose microspheres by pre-cross-linking and surfactant micelles swelling method. COLLOIDS AND SURFACES B-BIOINTERFACES[J]. 2019, 182: http://dx.doi.org/10.1016/j.colsurfb.2019.110377.
[21] Qi, Feng, Wu, Jie, Li, Hao, Ma, Guanghui. Recent research and development of PLGA/PLA microspheres/nanoparticles: A review in scientific and industrial aspects. FRONTIERS OF CHEMICAL SCIENCE AND ENGINEERING[J]. 2019, 13(1): 14-27, http://lib.cqvip.com/Qikan/Article/Detail?id=7001649010.
[22] 吴楠, 吴颉, 苗春宇, 马光辉, 安文琪. 基于纳米氢氧化铝颗粒的Pickering乳液制备. 过程工程学报[J]. 2019, 19(6): 1220-, http://lib.cqvip.com/Qikan/Article/Detail?id=7100645785.
[23] Wu, Jie, Ma, Guanghui. Imitation of nature: Bionic design in the study of particle adjuvants. JOURNAL OF CONTROLLED RELEASE[J]. 2019, 303: 101-108, http://dx.doi.org/10.1016/j.jconrel.2019.04.004.
[24] Xia, Yufei, Na, Xiangming, Wu, Jie, Ma, Guanghui. The Horizon of the Emulsion Particulate Strategy: Engineering Hollow Particles for Biomedical Applications. ADVANCED MATERIALS[J]. 2019, 31(38): https://www.webofscience.com/wos/woscc/full-record/WOS:000486253200003.
[25] Xia, Yufei, Wu, Jie, Du, Yiqun, Miao, Chunyu, Su, Zhiguo, Ma, Guanghui. Bridging Systemic Immunity with Gastrointestinal Immune Responses via Oil-in-Polymer Capsules. ADVANCED MATERIALS[J]. 2018, 30(31): http://www.irgrid.ac.cn/handle/1471x/1764407.
[26] Wu, Sihua, Yue, Hua, Wu, Jie, Zhang, Wenming, Jiang, Min, Ma, Guanghui. The interacting role of physical stiffness and tumor cells on the macrophages polarization. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS[J]. 2018, 552: 81-88, http://dx.doi.org/10.1016/j.colsurfa.2018.04.026.
[27] Guanghui Ma, Jie Wu, Lianyan Wang, Yueqi Wang, Qi Liu. Uniform biodegradable nanoparticles with high pH-sensitivity as vaccine adjuvant. NANOMEDICINE: NANOTECHNOLOGY, BIOLOGY, AND MEDICINE. 2018, 14(5): 1882-1882, http://dx.doi.org/10.1016/j.nano.2017.11.374.
[28] Xia, Yufei, Wu, Jie, Wei, Wei, Du, Yiqun, Wan, Tao, Ma, Xiaowei, An, Wenqi, Guo, Aiying, Miao, Chunyu, Yue, Hua, Li, Shuoguo, Cao, Xuetao, Su, Zhiguo, Ma, Guanghui. Exploiting the pliability and lateral mobility of Pickering emulsion for enhanced vaccination. NATURE MATERIALS[J]. 2018, 17(2): 187-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000423153800019.
[29] 吴楠, 吴晶晶, 岳华, 吴颉, 马光辉. 壳聚糖季铵盐微球作为注射疫苗佐剂的安全性评价. 2016年全国高分子材料科学与工程研讨会论文摘要集null. 2016, 1-, http://www.irgrid.ac.cn/handle/1471x/6898742.
[30] Wang, YueQi, Liu, Yan, Wang, YuXia, Wu, YaJun, Jia, PeiYuan, Shan, JunJie, Wu, Jie, Ma, GuangHui, Su, ZhiGuo. The potential adjuvanticity of quaternized chitosan hydrogel based microparticles for porcine reproductive and respiratory syndrome virus inactivated vaccine. INTERNATIONAL IMMUNOPHARMACOLOGY[J]. 2016, 39(OCT): 84-91, http://dx.doi.org/10.1016/j.intimp.2016.07.012.
[31] Fan, Qingze, Qi, Feng, Miao, Chunyu, Yue, Hua, Gong, Fangling, Wu, Jie, Ma, Guanghui, Su, Zhiguo. Direct and controllable preparation of uniform PLGA particles with various shapes and surface morphologies. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS[J]. 2016, 500: 177-185, http://dx.doi.org/10.1016/j.colsurfa.2016.04.028.
[32] 邹勇娟, 刘艳, 王月琦, 吴颉, 巩方玲, 马光辉. 壳聚糖微球及其与白油混合乳液对禽用新流二联疫苗佐剂的免疫效果. 过程工程学报[J]. 2016, 16(4): "639-646", http://www.irgrid.ac.cn/handle/1471x/1188240.
[33] 吴颉. 新型疫苗佐剂及其进展. 2016第七届国际DNA和基因组活动周——2016第六届国际分子与细胞生物学大会、2016第一届国际遗传学大会会刊null. 2016, 1-, http://www.irgrid.ac.cn/handle/1471x/6898758.
[34] Wu, Jie, Ma, GuangHui. Recent Studies of Pickering Emulsions: Particles Make the Difference. SMALL[J]. 2016, 12(34): 4633-4648, http://ir.ipe.ac.cn/handle/122111/21332.
[35] Yuan YU, Xing-Lan WU, Yin LI, Yi-Kang HUANG, Cheng-Wei JIANG, Fei-Fei ZHAO, Jie WU, Rong-Yue ZHANG. Preparation of Mixed-mode Chromatography Supports Based on Gigaporous Polymer Microspheres. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY. 2016, 44(12): 1874-1879, http://dx.doi.org/10.1016/S1872-2040(16)60979-9.
[36] 邹勇娟, 刘艳, 王月琦, 吴颉, 巩方玲, 马光辉. 壳聚糖微球及其复合佐剂用作新流二联疫苗的免疫效果. 2016年全国高分子材料科学与工程研讨会论文摘要集null. 2016, 1-, http://www.irgrid.ac.cn/handle/1471x/6898743.
[37] Wang, YueQi, Fan, QingZe, Liu, Yan, Yue, Hua, Ma, XiaoWei, Wu, Jie, Ma, GuangHui, Su, ZhiGuo. Improving adjuvanticity of quaternized chitosan-based microgels for H5N1 split vaccine by tailoring the particle properties to achieve antigen dose sparing effect. INTERNATIONAL JOURNAL OF PHARMACEUTICS[J]. 2016, 515(1-2): 84-93, http://dx.doi.org/10.1016/j.ijpharm.2016.09.082.
[38] 吴颉. 快速膜乳化法与热固化法制备粒径均一的pH敏感壳聚糖季铵盐凝胶微球. 过程工程学报. 2015, [39] 刘艳, 王月琦, 范清泽, 吴有斌, 吴颉, 马光辉. 快速膜乳化与热固化法制备粒径均一的pH敏感壳聚糖季铵盐凝胶微球. 过程工程学报[J]. 2015, 15(3): 473-481, http://www.irgrid.ac.cn/handle/1471x/1047929.
[40] Xia, Yufei, Fan, Qingze, Hao, Dongxia, Wu, Jie, Ma, Guanghui, Su, Zhiguo. Chitosan-based mucosal adjuvants: Sunrise on the ocean. VACCINE[J]. 2015, 33(44): 5997-6010, http://dx.doi.org/10.1016/j.vaccine.2015.07.101.
[41] Qi, Feng, Yang, Liuqing, Wu, Jie, Ma, Guanghui, Su, Zhiguo. Microcosmic Mechanism of Dication for Inhibiting Acylation of Acidic Peptide. PHARMACEUTICAL RESEARCH[J]. 2015, 32(7): 2310-2317, http://www.irgrid.ac.cn/handle/1471x/975481.
[42] Wu, Jie, Wang, YueQi, Fan, QingZe, Wu, YouBin, Ma, GuangHui. Novel hydrogel adjuvant based on quaternized chitosan for H5N1 split vaccine. JOURNAL OF CONTROLLED RELEASE[J]. 2015, 213(SEP): E51-E52, http://dx.doi.org/10.1016/j.jconrel.2015.05.084.
[43] 夏宇飞, 吴颉, 苏志国. 粒径均一的胶体体微囊药物递送体系. 2015年中国化工学会年会论文集null. 2015, http://ir.ipe.ac.cn/handle/122111/20415.
[44] Wu, Jie, Fan, Qingze, Xia, Yufei, Ma, Guanghui. Uniform-sized particles in biomedical field prepared by membrane emulsification technique. CHEMICAL ENGINEERING SCIENCE[J]. 2015, 125(MAR): 85-97, http://dx.doi.org/10.1016/j.ces.2014.08.016.
[45] Sheng, Yifeng, Sun, Guanqing, Wu, Jie, Ma, Guanghui, Ngai, To. Silica-Based Liquid Marbles as Microreactors for the Silver Mirror Reaction. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION[J]. 2015, 54(24): 7012-7017, http://www.irgrid.ac.cn/handle/1471x/975494.
[46] Sun, Guanqing, Qi, Feng, Wu, Jie, Ma, Guanghui, Ngai, To. Preparation of Uniform Particle-Stabilized Emulsions Using SPG Membrane Emulsification. LANGMUIR[J]. 2014, 30(24): 7052-7056, http://www.irgrid.ac.cn/handle/1471x/857749.
[47] Nan, Fangfang, Wu, Jie, Qi, Feng, Fan, Qingze, Ma, Guanghui, Ngai, To. Preparation of uniform-sized colloidosomes based on chitosan-coated alginate particles and its application for oral insulin delivery. JOURNAL OF MATERIALS CHEMISTRY B[J]. 2014, 2(42): 7403-7409, http://www.irgrid.ac.cn/handle/1471x/944871.
[48] Zhao, Xi, Wu, Jie, Gong, FangLing, Cui, JinMei, Janson, JanChrister, Ma, GuangHui, Su, ZhiGuo. Preparation of uniform and large sized agarose microspheres by an improved membrane emulsification technique. POWDER TECHNOLOGY[J]. 2014, 253(1): 444-452, http://dx.doi.org/10.1016/j.powtec.2013.12.012.
[49] Nan, Fangfang, Wu, Jie, Qi, Feng, Liu, Yan, Ngai, To, Ma, Guanghui. Uniform chitosan-coated alginate particles as emulsifiers for preparation of stable Pickering emulsions with stimulus dependence. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS[J]. 2014, 456(8m): 246-252, http://dx.doi.org/10.1016/j.colsurfa.2014.05.017.
[50] Wang, YueQi, Wu, Jie, Fan, QingZe, Zhou, Meng, Yue, ZhanGuo, Ma, GuangHui, Su, ZhiGuo. Novel Vaccine Delivery System Induces Robust Humoral and Cellular Immune Responses Based on Multiple Mechanisms. ADVANCED HEALTHCARE MATERIALS[J]. 2014, 3(5): 670-681, http://www.irgrid.ac.cn/handle/1471x/857775.
[51] Qi, Feng, Wu, Jie, Hao, Dongxia, Yang, Tingyuan, Ren, Yu, Ma, Guanghui, Su, Zhiguo. Comparative Studies on the Influences of Primary Emulsion Preparation on Properties of Uniform-Sized Exenatide-Loaded PLGA Microspheres. PHARMACEUTICAL RESEARCH[J]. 2014, 31(6): 1566-1574, http://www.irgrid.ac.cn/handle/1471x/857739.
[52] Qi, Feng, Wu, Jie, Sun, Guanqing, Nan, Fangfang, Ngai, To, Ma, Guanghui. Systematic studies of Pickering emulsions stabilized by uniform-sized PLGA particles: preparation and stabilization mechanism. JOURNAL OF MATERIALS CHEMISTRY B[J]. 2014, 2(43): 7605-7611, http://www.irgrid.ac.cn/handle/1471x/945000.
[53] Li, Xiaodong, Sun, Guanqing, Li, Yecheng, Yu, Jimmy C, Wu, Jie, Ma, GuangHui, Ngai, To. Porous TiO2 Materials through Pickering High-Internal Phase Emulsion Templating. LANGMUIR[J]. 2014, 30(10): 2676-2683, http://www.irgrid.ac.cn/handle/1471x/837068.
[54] 杨柳青, 王丽秋, 吴颉, 齐峰, 任玉, 陶安进, 马亚平, 马光辉. 快速膜乳化法制备胸腺法新长效缓释微球. 过程工程学报[J]. 2014, 14(6): 994-999, http://www.irgrid.ac.cn/handle/1471x/945654.
[55] Sun, Guanqing, Sheng, Yifeng, Wu, Jie, Ma, Guanghui, Ngai, To. Liquid Marbles Stabilized by Charged Polymer Latexes: How Does the Drying of the Latex Particles Affect the Properties of Liquid Marbles?. LANGMUIR[J]. 2014, 30(42): 12503-12508, http://www.irgrid.ac.cn/handle/1471x/945011.
[56] Qi, Feng, Wu, Jie, Yang, Tingyuan, Ma, Guanghui, Su, Zhiguo. Mechanistic studies for monodisperse exenatide-loaded PLGA microspheres prepared by different methods based on SPG membrane emulsification. ACTA BIOMATERIALIA[J]. 2014, 10(10): 4247-4256, http://dx.doi.org/10.1016/j.actbio.2014.06.018.
[57] 吴颉. Mechanism Studies for Monodisperse Exenatide-loaded PLGA Microspheres Prepared by Different Methods Based on SPG Membrane emulsification. Acta Biomaterialia. 2014, [58] Qi, Feng, Wu, Jie, Fan, Qingze, He, Fan, Tian, Guifang, Yang, Tingyuan, Ma, Guanghui, Su, Zhiguo. Preparation of uniform-sized exenatide-loaded PLGA microspheres as long-effective release system with high encapsulation efficiency and bio-stability. COLLOIDS AND SURFACES B-BIOINTERFACES[J]. 2013, 112: 492-498, http://dx.doi.org/10.1016/j.colsurfb.2013.08.048.
[59] 吴颉. Preparation of uniform-sized exenatide-loaded PLGA microspheres as long-effective release system with high encapsulation ef?ciency and bio-stability. Colloids and Surfaces B: Biointerfaces. 2013, [60] 何帆, 齐峰, 吴颉, 苏志国. 快速膜乳化法制备载生长激素释放肽-6的PLGA微球. 过程工程学报[J]. 2013, 13(3): 458-465, http://www.irgrid.ac.cn/handle/1471x/856738.
[61] Wu, Youbin, Wu, Shipo, Hou, Lihua, Wei, Wei, Zhou, Meng, Su, Zhiguo, Wu, Jie, Chen, Wei, Ma, Guanghui. Novel thermal-sensitive hydrogel enhances both humoral and cell-mediated immune responses by intranasal vaccine delivery. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS[J]. 2012, 81(3): 486-497, http://dx.doi.org/10.1016/j.ejpb.2012.03.021.
[62] 赵希, 吴颉, 马光辉, 苏志国. 粒径均一的海洋多糖微球制备技术及应用. 中国科技成果[J]. 2012, 41-43, http://lib.cqvip.com/Qikan/Article/Detail?id=41782637.
[63] Wu, Youbin, Wei, Wei, Zhou, Meng, Wang, Yueqi, Wu, Jie, Ma, Guanghui, Su, Zhiguo. Thermal-sensitive hydrogel as adjuvant-free vaccine delivery system for H5N1 intranasal immunization. BIOMATERIALS[J]. 2012, 33(7): 2351-2360, http://dx.doi.org/10.1016/j.biomaterials.2011.11.068.
[64] Wei, Wei, Ma, GuangHui, Wang, LianYan, Wu, Jie, Su, ZhiGuo. Hollow quaternized chitosan microspheres increase the therapeutic effect of orally administered insulin. ACTA BIOMATERIALIA[J]. 2010, 6(1): 205-209, http://dx.doi.org/10.1016/j.actbio.2009.06.005.
[65] 马光辉, 王连艳, 魏炜, 吴颉, 张岳玲, 吕坯平, 苏志国. 用于蛋白质多肽药物给药的壳聚糖微球的制备和药物活性保持策略. 2010年全国高分子材料科学与工程研讨会学术论文集（上册）null. 2010, 2-, http://www.irgrid.ac.cn/handle/1471x/717276.
[66] Ma GuangHui, Yang Jian, Lv PiPing, Wang LianYan, Wei Wei, Tian Rui, Wu Jie, Su ZhiGuo. Preparation of Uniform Microspheres and Microcapsules by Modified Emulsification Process. MACROMOLECULAR SYMPOSIAnull. 2010, 288: 41-48, [67] 吴颉, 吴有斌, 王月琦, 王豪, 马光辉. 结合膜乳化及热固化技术制备壳聚糖季铵盐凝胶颗粒. 生物颗粒与粉体制备、应用技术研讨会论文集null. 2010, 2-, http://www.irgrid.ac.cn/handle/1471x/717347.
[68] 马光辉. 利用快速膜乳化法制备粒径均一的PLGA微球和微囊. 过程工程学报. 2009, [69] 林楠, 吴颉, 郑国钧, 马光辉, 苏志国. 微孔膜乳化法制备大粒径琼脂糖微球. 过程工程学报[J]. 2009, 953-961, http://lib.cqvip.com/Qikan/Article/Detail?id=31794201.
[70] Wei, Wei, Yuan, Lan, Hu, Gang, Wang, LianYan, Wu, He, Hu, Xue, Su, ZhiGuo, Ma, GuangHui. Monodisperse chitosan microspheres with interesting structures for protein drug delivery. ADVANCED MATERIALS[J]. 2008, 20(12): 2292-+, http://www.irgrid.ac.cn/handle/1471x/725373.
[71] Wei, Wei, Wang, LianYan, Wu, Jie, Ma, GuangHui. Quaternized chitosan microspheres as insulin carrier. JOURNAL OF BIOTECHNOLOGYnull. 2008, 136: S452-S452, http://dx.doi.org/10.1016/j.jbiotec.2008.07.1050.
[72] Wu, Jie, Wei, Wei, Wang, LianYan, Su, ZhiGuo, Ma, GuangHui. Preparation of uniform-sized pH-sensitive quaternized chitosan microsphere by combining membrane emulsification technique and thermal-gelation method. COLLOIDS AND SURFACES B-BIOINTERFACES[J]. 2008, 63(2): 164-175, http://dx.doi.org/10.1016/j.colsurfb.2007.11.021.
[73] Wu, Jie, Wei, Wei, Wang, LianYan, Su, ZhiGuo, Ma, GuangHui. A thermosensitive hydrogel based on quaternized chitosan and poly(ethylene glycol) for nasal drug delivery system. BIOMATERIALS[J]. 2007, 28(13): 2220-2232, http://dx.doi.org/10.1016/j.biomaterials.2006.12.024.
[74] Wu, Jie, Su, ZhiGuo, Ma, GuangHui. A thermo- and pH-sensitive hydrogel composed of quaternized chitosan/glycerophosphate. INTERNATIONAL JOURNAL OF PHARMACEUTICS[J]. 2006, 315(1-2): 1-11, http://www.irgrid.ac.cn/handle/1471x/736364.
[75] 吴颉, 魏炜, 马光辉, 苏志国. 新型鼻腔黏膜给药体系的研究. 第三届全国化学工程与生物化工年会论文摘要集（下）null. 2006, 1-, http://www.irgrid.ac.cn/handle/1471x/717346.
[76] 景晓燕, 吴颉, 王君, 张密林. 磁性PF微球的制备及性质. 哈尔滨工程大学学报[J]. 2003, 24(3): 344-346,354, http://lib.cqvip.com/Qikan/Article/Detail?id=8048470.
[77] 吴颉, 王君, 景晓燕, 张密林. 磁性聚乙烯醇缩丁醛微球的制备和性质. 化工新型材料[J]. 2003, 31(3): 13-16, http://lib.cqvip.com/Qikan/Article/Detail?id=7612728.
[78] 吴颉, 王君, 张密林. 磁性聚乙烯醇缩丁醛微球固定化α—淀粉酶. 精细化工[J]. 2003, 20(3): 143-145,156, http://lib.cqvip.com/Qikan/Article/Detail?id=7501673.
[79] 王君, 景晓燕, 张密林, 吴颉. 磁性高分子微球的制备与应用. 化工新型材料[J]. 2002, 30(8): 23-26, http://lib.cqvip.com/Qikan/Article/Detail?id=6709050.

   

（ 1 ） 用作DNA疫苗载体的壳聚糖季铵盐纳微球的新型制备方法及应用研究, 主持, 国家级, 2010-01--2012-12
（ 2 ） 新型壳聚糖纳微球制备及其作为黏膜免疫佐剂的研究, 主持, 省级, 2011-01--2013-12
（ 3 ） 疫苗新佐剂和新剂型的研发, 参与, 部委级, 2011-01--2013-12
（ 4 ） 新型纳微颗粒佐剂及黏膜免疫佐剂的研究, 主持, 国家级, 2014-01--2016-12
（ 5 ） 海洋琼脂及甲壳质衍生物高值化产品开发, 参与, 国家级, 2013-01--2016-12
（ 6 ） 智能均一胶体体微囊的研制及其在药物释放中的应用, 参与, 国家级, 2012-01--2014-12
（ 7 ） 生物技术药物优化技术体系, 参与, 部委级, 2014-05--2015-12
（ 8 ） 温敏型壳聚糖水凝胶佐剂及新型H5N1疫苗制剂开发, 主持, 研究所（学校）, 2014-06--2016-06
（ 9 ） 具有双重免疫增强特性的Pickering乳液构建及其调控机制研究, 主持, 国家级, 2016-01--2019-12
（ 10 ） 可诱导双重免疫应答的硬乳液剂型构建及机制研究, 主持, 国家级, 2018-01--2021-12
（ 11 ） 海洋源功能生物材料及医药制剂开发, 参与, 部委级, 2018-01--2019-12
（ 12 ） 海洋鱼类浸泡、口服型疫苗的探索性研究, 主持, 国家级, 2018-08--2021-12

（1）疫苗颗粒佐剂研究   2018生物大分子药物论坛   2018-10-26
（2）仿生颗粒乳液制剂研究   国家自然科学基金委员会化学科部第八届化学工程青年者术交流研讨会   2018-09-11
（3）Our Study for Mucosal Immune Adjuvant   第1届中日颗粒论坛   2018-08-09
（4）仿生颗粒-乳液剂型研究   中国材料大会   2018-07-13
（5）仿生颗粒-乳液剂型研究   2018第五届生物化工技术创新及产业发展研讨会暨第二届生物化工青年学者论坛   2018-05-11
（6）壳聚糖凝胶及颗粒作为疫苗佐剂的研究   第一届天然材料研究与应用研讨会   2017-11-10
（7）Pickering Emulsions in Biomedical Field: Particles Decide the Difference   2017-10-15
（8）Preparation and Investigation of Uniform Chitosan Particles as Vaccine Adjuvant   2015-12-08
（9）新型疫苗佐剂和递送系统研究   2015动物疫苗合作论坛   吴颉   2015-07-09
（10）The application of chitosan thermal sensitive hydrogel as vaccine adjuvant both for nasal administration and injection   1) Jie Wu, Yueqi Wang, Qingze Fan, Youbin Wu, Guanghui Ma   2014-11-06
（11）Novel hydrogel adjuvant based on quaternized chitosan for H5N1 split vaccine   Jie Wu, Yue-Qi Wang, Qing-Ze Fan, You-Bin Wu, Guang-Hui Ma   2014-09-16
（12）Preparation and Pharmacological Evaluation of Uniform-sized Drug-loaded PLGA Microspheres by SPG Membrane Emulsification   Jie Wu*, Feng Qi, Guang-Hui Ma   2014-07-13
（13）Chitosan-based microgels as vaccine adjuvants   Jie Wu*, Yue-Qi Wang, Qiang-Ze Fan, Guang-Hui Ma   2014-05-18
（14）可发挥多重免疫增强作用机制的新型纳微佐剂研究   2013年我国纳米生物与医药技术发展研讨会暨中国微米纳米技术学会纳米科学技术分会   吴颉，王月琦，范清泽，马光辉*，苏志国   2013-11-30
（15）壳聚糖温敏材料作为疫苗佐剂的设计、制备和应用   2013中国化工学会年会   吴颉*，王月琦，范清泽，马光辉   2013-09-23
（16） The preparation of HTCC temperature sensitive hydrogel and its application in nasal immunization   Jie Wu*, You-Bin Wu, Meng Zhou, Guang-Hui Ma   2012-11-08
（17）采用快速膜乳化技术制备粒径均一的壳聚糖纳微颗粒   中国微米纳米技术学会纳米科学技术分会纳米生物与医药技术专业委员会2010学术年会   吴颉，吴有斌，王月琦，王豪，马光辉   2010-10-26
（18）结合膜乳化及热固化技术制备壳聚糖季铵盐凝胶颗粒   生物颗粒与粉体制备、应用技术研讨会   吴颉，吴有斌，王月琦，王豪，马光辉   2010-09-27
（19）微孔膜乳化法制备大粒径琼脂糖微球   全国第八届海洋生物技术与创新药物学术讨论会   吴颉，赵希，林楠，马光辉，苏志国   2010-08-08
（20）PREPARATION UNIFORM-SIZED QUATERNIZED CHITOSAN MICRO- AND NANO-SPHERES BY COMBINING MEMBRANE EMULSIFICATION TECHNIQUE AND THERMAL-GELATION METHOD   吴颉，吴有斌，王月琦，魏炜，马光辉   2010-05-22
（21）壳聚糖季铵盐智能水凝胶的制备及其应用   全国生物材料大会   吴颉，吴有斌，魏炜，王月琦，马光辉   2010-04-15
（22）Preparation of Intelligent Quaternized Chitosan Hydrogel and Its Application as Drug Delivery System   第四届国际药物制剂论坛   吴颉，魏炜，马光辉，苏志国   2009-09-24
（23）Uniform-sized Quaternized Chitosan Microsphere Prepared By Combining Membrane Emulsification Technique And Thermal-gelation Method   吴颉，魏炜，王连艳，苏志国，马光辉   2009-04-25
（24）Preparation of Thermal-sensitive Quaternized Chitosan Hydrogel and Its Application as Nasal Drug Delivery System   海内外青年学者过程工程前沿与发展学术研讨会——面向可持续发展的过程工程   吴颉，马光辉，苏志国，魏炜，王连艳   2008-09-26
（25）Preparation of Thermal-sensitive Quaternized Chitosan Hydrogel and Its Application as Nasal Drug Delivery System   吴颉，马光辉，苏志国，魏炜，王连艳   2007-12-06
（26）Preparation of uniform-sized pH-sensitive quaternized chitosan microsphere by combining membrane emulsification technique and thermal-gelation method   吴颉，魏炜，王连艳，苏志国，马光辉   2007-09-09
（27）Preparation of uniform-sized pH-sensitive quaternized chitosan microspheres by a new method   吴颉，魏炜，王连艳，苏志国，马光辉   2007-08-29

现指导学生

屈艳玲  硕士研究生  081703-生物化工