Important biological agents biosynthesis and metabolic regulation

09/1999-07/2002   Ph.D, Microbiology, College of life science, Nankai University. Tianjin,China.

09/1986-07/1989   MS, Microbiology, Department of Biology, Nankai University. Tianjin, China.

09/1982-07/1986   BS, Microbiology, Department of Biology, Nankai University. Tianjin, China.

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11/2005-Present  Professor, Principal Investigator of Metabolic Engineering and Systems Biology of Industrial Microbes, CAS Key Laboratory of Pathogenic Microbiology & Immunology, Institute of Microbiology, Chinese Academy of Sciences.

01/2004-09/2005  Post-doc researcher, Institut de Biologie Physico-Chimque, Centre National de la Recherche Scientifique(CNRS UPR9073), Paris, France.

08/1989-01/2004    Professor, Director of Molecular Biology Laboratory, The First Teaching Hospital, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

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11/2005-Present  Professor, Principal Investigator of Metabolic Engineering and Systems Biology of Industrial Microbes, CAS Key Laboratory of Pathogenic Microbiology & Immunology, Institute of Microbiology, Chinese Academy of Sciences.

01/2004-09/2005  Post-doc researcher, Institut de Biologie Physico-Chimque, Centre National de la Recherche Scientifique(CNRS UPR9073), Paris, France.

08/1989-01/2004    Professor, Director of Molecular Biology Laboratory, The First Teaching Hospital, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

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 Selected Publications:                         

1.Aihua Deng, Zhaopeng Sun, Tiantian Wang, Di Cui，Lai Li , Shuwen Liu, Fei Huang and Tingyi Wen*. Simultaneous Multiplex Genome Engineering via Accelerated Natural Transformation in Bacillus subtilis. Frontiers in Microbiology. 2021; 12:714449.  doi: 10.3389/fmicb.2021.714449.

2.Dalan Jing†, Aihua Deng†, Hongmei Wang†, Yilin Chou, Xiaodan Jiang, Zhenxiang Chen, Xuemin Li* and Tingyi Wen*. Comparative Evaluation of Bandage Contact Lenses and Eye Patching after Bilateral Cataract Surgery. Journal of Ophthalmology. 2021，Article ID 2873543. doi:10.1155/2021/2873543.

3.Hua Bai†, Huibin Lv†, Aihua Deng, Xiaodan Jiang, Xuemin Li* and Tingyi Wen*. Lysobacter oculi sp. nov., isolated from human Meibomian gland secretions. Antonie van Leeuwenhoek. 2020;113（1）：13-20. doi: 10.1007/s10482-019-01289-1.

4.Shuwen Liu†, Haihan Xiao†, Fangfang Zhang, Zheng Lu, Yun Zhang, Aihua Deng, Zhongcai Li, Cui Yang and Tingyi Wen*.  A seamless and iterative DNA assembly method named PS‑Brick and its assisted metabolic engineering for threonine and 1‑propanol production. Biotechnol Biofuels. 2019,12:180.

5.Di Cui, Aihua Deng, Hua Bai, Zhao Yang, Yong Liang, Zhe Liub, Qidi Qiu, Laiyou Wang, Shuwen Liu, Yun Zhang, Yi Shi, Jianxun Qi* and Tingyi Wen*. Molecular basis for feedback inhibition of tyrosine-regulated 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Escherichia coli. Journal of Structural Biology, 2019；206:322-334. doi: 10.1016/j.jsb.2019.04.001.

6.Yu Zhang, Yun Zhang*, Xiuling Shang, Bo Wang, Qitiao Hu, Shuwen Liu, Tingyi Wen*. Reconstruction of TCA cycle in Corynebacterium glutamicum with a genome-scale metabolic network model for trans-4-hydroxyproline production. Biotechnology and Bioengineering. 2019; 116:99–109. doi:10.1002/bit.26818.

7.Laiyou Wang, Aihua Deng*, Yun Zhang1, Shuwen Liu, Yong Liang, Hua Bai, Di Cui, Qidi Qiu, Xiuling Shang, Zhao Yang, Xiuping He and Tingyi Wen*. Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts.  Biotechnology for Biofuels. 2018,11:277. doi. 10.1186/s13068-018-1271-0.

8.Xiaodan Jiang#, Aihua Deng#, Jiarui Yang#, Hua Bai, Zhao Yang, Jie Wu, Huibin Lv, Xuemin Li *, Tingyi Wen*. Pathogens in the Meibomian Gland and Conjunctival Sac: Microbiome of Normal Subjects and Patients with Meibomian Gland Dysfunction. Infection and Drug Resistance. 2018,11: 1729-1740. doi:10.2147/IDR.S162135.

9.Bo Wang^#, Qitiao Hu^#, Yu Zhang, Ruilin Shi, Xin Chai, Zhe Liu, Xiuling Shang, Yun Zhang*, Tingyi Wen*. A RecET-assisted CRISPR-Cas9 genome editing in Corynebacterium glutamicum. Microbial Cell Factories. 2018, 17：63. doi: 10.1186/s12934-018-0910-2.

10. Jie Wu^#, Aihua Deng^#, Qinyun Sun, Hua Bai, Zhaopeng Sun, Xiuling Shang, Yun Zhang, Qian Liu, Yong Liang, Shuwen Liu, Yongsheng Che* and Tingyi Wen*. Bacterial Genome Editing via a Designed Toxin−Antitoxin Cassette. ACS Synthetic Biology.  2018, 7 (3): 822–831. doi: 10.1021/acssynbio.6b00287（cover article）.

11. Yun Zhang, Xiuling Shang, Shujuan Lai, Yu Zhang, Qitiao Hu, Xin Chai, Bo Wang, Shuwen Liu, Tingyi Wen*. Reprogramming one-carbon metabolic pathways to decouple l-serine catabolism from cell growth in Corynebacterium glutamicum. ACS Synthetic Biology.  2018, 7 (2): 635–646. doi: 10.1021/acssynbio.7b00373.

12. Xiuling Shang#, Xin Chai#, Xuemei Lu，Yuan Li，Yun Zhang, Guoqiang Wang, Chen Zhang, Shuwen Liu, Yu Zhang, Jiyin Ma, Tingyi Wen* . Native promoters of Corynebacterium glutamicum and its application in l-lysine production. Biotechnology letters. 2018，40(2): 383-391. doi:10.1007/s10529-017-2479-y.

13. Hua Bai, Aihua Deng*, Shuwen Liu,Di Cui, Qidi Qiu, Laiyou Wang, Zhao Yang, Jie Wu, Xiuling Shang, Yun Zhang, and   Tingyi Wen*. A Novel Tool for Microbial Genome Editing Using the Restriction-Modification System. ACS Synthetic Biology. 2018， 7 (1): 98-106. doi: 10.1021/acssynbio.7b00254.

14. Zhao Yang, Luyun He, Kaisu Lin, Yun Zhang, Aihua Deng, Yong Liang, Chong Li* and Tingyi Wen*. The KMT1A-GATA3-STAT3 circuit is a novel self-renewal signaling of human bladder cancer stem cells. Clinical Cancer Research. 2017,23(21): 6673–6685. doi:10.1158/1078-0432.CCR-17-0882.

15. Yu Zhang, Jingyi Cai, Xiuling Shang, Bo Wang, Shuwen Liu, Xin Chai, Tianwei Tan, Yun Zhang* and Tingyi Wen*. A new genome‑scale metabolic model of Corynebacterium glutamicum and its application. Biotechnology for Biofuels. 2017,10:169.

16. Xin Chai, Xiuling Shang, Yu Zhang, Shuwen Liu, Yong Liang, Yun Zhang* and Tingyi Wen*.  A novel pyruvate kinase and its application in lactic acid production under oxygen deprivation in Corynebacterium glutamicum. BMC Biotechnology.  2016,16:79.

17. Aihua Deng, Wei Lin, Nana Shi, Jie Wu, Zhaopeng Sun, Qinyun Sun, Hua Bai, Yongxin Pan, Tingyi Wen*. In vitro assembly of the bacterial actin protein MamK from ‘Candidatus Magnetobacterium casensis’ in the phylum Nitrospirae. Protein & Cell. 2016, 7(4): 267–280.

18. Qian Liu, Yong Liang, Yun Zhang,Xiuling Shang, Shuwen Liu, Jifu Wen, Tingyi Wen*. YjeH Is a Novel Exporter of l-Methionine and Branched-Chain Amino Acids in Escherichia coli. Applied and Environmental Microbiology. 2015, 81(22):7753-7766.

19. Zhaopeng Sun, Aihua Deng, Ting Hu, Jie Wu, Qinyun Sun, Hua Bai, Guoqiang Zhang, Tingyi Wen*. A high efficiency recombineering system with PCR-based ssDNA in Bacillus subtilis mediated by the native phage recombinase GP35. Applied Microbiology and Biotechnology. 2015, 99(12): 5151-5162.

20. Aihua Deng, Guoqiang Zhang, Nana Shi, Jie Wu, Fuping Lu, Tingyi Wen*. Secretory expression, functional characterization and molecular genetic analysis of a novel halo-solvent-tolerant protease from Bacillus gibsonii. Journal of Microbiology and Biotechnology. 2014, 24(2): 197-208.

21. Xiuling Shang^#, Yun Zhang^#, Guoqiang Zhang, Xin Chai, Aihua Deng, YongLiang, Tingyi Wen*. Characterization and Molecular Mechanism of AroP as an Aromatic Amino Acid and Histidine Transporter in Corynebacterium glutamicum. Journal of Bacteriology. 2013, 195(23): 5334-5342.     

22. Shuwen Liu, Yong Liang, Qian Liu, Tongtong Tao, Shujuan Lai, Ning Chen, Tingyi Wen*. Development of a two-stage feeding strategy based on the kind and level of feeding nutrients for improving fed-batch production of L-threonine by Escherichia coli. Applied Microbiology and Biotechnology. 2013, 97(2):573–583.  

23. Guoqiang Zhang, Wenzhao Wang, Aihua Deng, Zhaopeng Sun, Yun Zhang, Yong Liang, Yongsheng Che, Tingyi Wen*. A Mimicking-of-DNA-methylation-patterns Pipeline for Overcoming the Restriction Barrier of Bacteria. Plos Genetics .2012, 8(9): e1002987.  

24. Yun Zhang, Xiuling Shang, Shujuan Lai, Guoqiang Zhang, Yong Liang, Tingyi Wen*. Development and application of an arabinose-inducible expression system by facilitating inducer uptake in Corynebacterium glutamicum. Applied and Environmental Microbiology. 2012, 78(16): 5831-5838.   

25. Aihua Deng, Zhaopeng. Sun, Guoqiang. Zhang, Jie Wu, Tingyi Wen*. Rapid discrimination of newly isolated Bacillales with industrial applications using Raman spectroscopy. Laser Physics Letters. 2012, 9(9), 636–642.  

26. LAI ShuJuan, ZHANG Yun, LIU ShuWen, LIANG Yong, SHANG XiuLing, CHAI Xin, WEN TingYi*. Metabolic engineering and flux analysis of Corynebacterium glutamicum for L-serine production. SCIENCE CHINA Life Sciences. 2012, 55(4): 283–290(Cover Article).

27. Yun Zhang, Xiuling Shang, Aihua Deng, Xin Chai, Shujuan Lai, Guoqiang Zhang, Tingyi Wen*. Genetic and biochemical characterization of Corynebacterium glutamicum ATP phosphoribosyltransferase and its three mutants resistant to feedback inhibition by histidine. Biochimie. 2012, 94(3): 829-838.    

28. Haojian Li^#，Guoqiang Zhang^#，Aihua Deng，Ning Chen，Tingyi Wen*. De novo engineering and metabolic flux analysis of Bacillus subtilis for inosine biosynthesis. Biotechnology letters. 2011, 33(8): 1575–1580.  

29. Aihua Deng, Jie Wu, Guoqiang Zhang, Tingyi Wen*. Molecular and structural characterization of a surfactant-stable high-alkaline protease AprB with a novel structural feature unique to subtilisin family. Biochimie. 2011, 93(4):783-791.

30. Guoqiang Zhang, Peng Bao, Yun Zhang, Aihua Deng, Ning Chen, Tingyi Wen*. Enhancing electro-transformation competency of recalcitrant Bacillus amyloliquefaciens by combining cell-wall weakening and cell-membrane fluidity disturbing. Analytical Biochemistry. 2011, 409(1):130–137.

31.Zhongyuan Liu, Qiangjun Zhou, Xinfang Mao, Xiangdong Zheng, Jiubiao Guo, Fuchun Zhang , Tingyi Wen* and Hai Pang*. Crystallization and preliminary X-ray analysis of cecropin B from Bombyx mori. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010, 66: 851-853.

32. Aihua Deng, Jie Wu, Yun Zhang, Guoqiang Zhang, Tingyi Wen*. Purification and characterization of a surfactant-stable high-alkaline protease from Bacillus sp. B001. Bioresource Technology. 2010, 101(18): 7100-7106.

33.Ning Chen, Jin Huang, Zhibin Feng, Lei Yu, Qingyang Xu, Tingyi Wen*. Optimization of fermentation conditions for the biosynthesis of L-threonine by Escherichia coli. Applied Biochemistry and Biotechnology. 2009, 158(3): 595-604.

Current research interests of our group focus on the construction of genome-scale metabolic model to design metabolic engineering strategy. Guided by in silico simulation, the biosynthesis and regulatory mechanisms of the amino acids, nucleotides and derivatives in the microbial and eucaryotic cells are investigated using systems biology and synthetic biology methods, to clarify the relationship between the amino acids, nucleotides metabolism and tumorigenesis as well as metabolic disease. Crystal structures of key enzymes involving in important life and metabolic processes are analyzed to guide directed evolution for improving enzymatic characteristics. The synthetic biology technologies including efficient genomic editing, regulatory elements and biosensors are developed to reconstruct cell factory for the production of bio-based products (amino acids, nucleotides and organic amines et. al.) and biological agents (vaccine, antibody, small-molecular inhibitor of tumor and biotoxin et. al.).

Key words of research interests:  industrial microbiology, metabolic engineering, genetic engineering, genome editing, gene expression and regulation，amino acids and derivatives, biological fermentation

已指导学生

邓爱华  博士研究生  071007-遗传学  

来书娟  硕士研究生  071007-遗传学  

张国强  博士研究生  071007-遗传学  

商秀玲  硕士研究生  071007-遗传学  

柴欣  博士研究生  071007-遗传学  

刘树文  博士研究生  071007-遗传学  

吴杰  博士研究生  071007-遗传学  

孙召朋  博士研究生  071007-遗传学  

温际富  硕士研究生  085238-生物工程  

孙钦云  硕士研究生  071007-遗传学  

张宇  博士研究生  071007-遗传学  

邱起地  硕士研究生  071007-遗传学  

王博  硕士研究生  071007-遗传学  

崔迪  博士研究生  071007-遗传学  

米杰  硕士研究生  085238-生物工程  

胡启跳  硕士研究生  085238-生物工程  

王来友  博士研究生  071007-遗传学  

白桦  博士研究生  071007-遗传学  

赵菁  硕士研究生  085238-生物工程  

现指导学生

肖海涵  博士研究生  071007-遗传学  

李来  博士研究生  071007-遗传学  

李忠财  博士研究生  071007-遗传学  

师瑞琳  博士研究生  071007-遗传学  

菅慧  博士研究生  071007-遗传学  

王雪亮  博士研究生  071007-遗传学