基本信息
王志珍  女  博导  中国科学院生物物理研究所
电子邮件: chihwang@sun5.ibp.ac.cn
通信地址: 北京市朝阳区大屯路15号
邮政编码: 100101

招生信息

   
招生专业
071010-生物化学与分子生物学
招生方向
内质网蛋白质氧化折叠系统及其调控以及与疾病和衰老的联系
蛋白质氧化折叠机制以及分子伴侣在蛋白质质量控制中的作用

教育背景

1959-09--1964-07   中国科学技术大学   大学本科

工作经历

   
工作简历
1998-02~1998-04,香港科技大学, 访问教授
1995-05~1995-08,德国哥丁根大学, 访问科学家
1991-05~1993-01,加拿大阿尔伯特大学, Research Associate
1988-08~1991-04,美国食品和药物管理局, 访问科学家
1987-05~1988-07,美国希望城国立医学中心, 访问科学家
1981-02~1982-05,美国国立健康研究院, Fogarty 访问学者
1979-02~1981-02,德国羊毛研究所, Humboldt访问学者
1964-07~现在, 中国科学院生物物理研究所, 助理研究员,副研究员,研究员,博士生导师
社会兼职
2013-12-01-今,中国女科技工作者协会, 会长
2013-03-24-今,茅以升科技教育基金会, 主任
2012-02-10-今,北京市科协, 副主席
2006-07-31-2010-07-30,中国生物化学和分子生物学会蛋白质专业委员会, 主任

专利与奖励

   
奖励信息
(1) 第六届中国十大女杰, , 国家级, 2007
(2) 何梁何利基金科学与技术进步奖, , 其他, 2005
(3) 国家自然科学奖二等奖, 二等奖, 国家级, 2002
(4) 第三世界科学院基础科学(生物学)奖, , 其他, 2002
(5) 中国科学院自然科学奖一等奖, 一等奖, 其他, 1999
(6) 国家自然科学奖二等奖, 二等奖, 国家级, 1995

出版信息

   
发表论文
[1] Fenggui Fan, Qiao Zhang, Yini Zhang, Guozhong Huang, Xuelian Liang, Chih-Chen Wang, Lei Wang, Dongping Lu. Two protein disulfide isomerase subgroups work synergistically in catalyzing oxidative protein folding. Plant Physiology[J]. 2022, 188(1): 241-254, https://pubmed.ncbi.nlm.nih.gov/34609517/.
[2] Lu Wang, Xi Wang, Xiying Lv, Qiushuo Jin, Hongcai Shang, Chih-chen Wang, Lei Wang. The extracellular Ero1α/PDI electron transport system regulates platelet function by increasing glutathione reduction potential. Redox Biology[J]. 2022, 50: [3] Wang, Lei, Yu, Jiaojiao, Wang, Chihchen. Protein disulfide isomerase is regulated in multiple ways: Consequences for conformation, activities, and pathophysiological functions. BIOESSAYS[J]. 2021, 43(3): https://www.webofscience.com/wos/woscc/full-record/WOS:000585977400001.
[4] Xinxin Chen, Jianchao Zhang, Pulan Liu, Yangyang Wei, Xie Wang, Junyu Xiao, Chihchen Wang, Lei Wang. Proteolytic processing of secretory pathway kinase Fam20C by site-1 protease promotes biomineralization. Proceedings of the National Academy of Sciences of the United States of America[J]. 2021, 118(32): [5] Yu, Jiaojiao, Li, Tao, Liu, Yu, Wang, Xi, Zhang, Jianchao, Wang, Xie, Shi, Guizhi, Lou, Jizhong, Wang, Likun, Wang, Chihchen, VVang, Lei. Phosphorylation switches protein disulfide isomerase activity to maintain proteostasis and attenuate ER stress. EMBO JOURNAL[J]. 2020, 39(10): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232009/.
[6] Fan, Fenggui, Zhang, Yini, Huang, Guozhong, Zhang, Qiao, Wang, Chihchen, Wang, Lei, Lu, Dongping. AtERO1 and AtERO2 Exhibit Differences in Catalyzing Oxidative Protein Folding in the Endoplasmic Reticulum. PLANT PHYSIOLOGY[J]. 2019, 180(4): 2022-2033, http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000477951400023.
[7] Wang, Lu, Zhou, Junsong, Wang, Lei, Wang, Chihchen, Essex, David W. The b ' domain of protein disulfide isomerase cooperates with the a and a ' domains to functionally interact with platelets. JOURNAL OF THROMBOSIS AND HAEMOSTASIS[J]. 2019, 17(2): 371-382, https://www.webofscience.com/wos/woscc/full-record/WOS:000458294900015.
[8] Wu, Xun, Zhang, Lihui, Miao, Yutong, Yang, Juan, Wang, Xian, Wang, Chihchen, Feng, Juan, Wang, Lei. Homocysteine causes vascular endothelial dysfunction by disrupting endoplasmic reticulum redox homeostasis. REDOX BIOLOGY[J]. 2019, 20: 46-59, http://dx.doi.org/10.1016/j.redox.2018.09.021.
[9] Xu, Shili, Liu, Yajing, Yang, Kai, Wang, Hanxiao, Shergalis, Andrea, Kyani, Anahita, Bankhead, Armand, III, Tamura, Shuzo, Yang, Suhui, Wang, Xi, Wang, Chihchen, Rehemtulla, Alnawaz, Ljungman, Mats, Neamati, Nouri. Inhibition of protein disulfide isomerase in glioblastoma causes marked downregulation of DNA repair and DNA damage response genes. THERANOSTICS[J]. 2019, 9(8): 2282-2298, [10] Zhang, Yini, Li, Tao, Zhang, Lihui, Shangguan, Fugen, Shi, Guizhi, Wu, Xun, Cui, Ya, Wang, Xie, Wang, Xi, Liu, Yongzhang, Lu, Bin, Wei, Taotao, Wang, Chihchen, Wang, Lei. Targeting the functional interplay between endoplasmic reticulum oxidoreductin-1 alpha and protein disulfide isomerase suppresses the progression of cervical cancer. EBIOMEDICINE[J]. 2019, 41: 408-419, [11] Fang, Jingqi, Yang, Jiping, Wu, Xun, Zhang, Gangming, Li, Tao, Wang, Xie, Zhang, Hong, Wang, Chihchen, Liu, GuangHui, Wang, Lei. Metformin alleviates human cellular aging by upregulating the endoplasmic reticulum glutathione peroxidase 7. AGING CELL[J]. 2018, 17(4): https://www.webofscience.com/wos/woscc/full-record/WOS:000439767700010.
[12] Li, Huanhuan, Yang, Kai, Wang, Wenjia, Niu, Yingbo, Li, Jun, Dong, Yuhui, Liu, Yingfang, Wang, Chihchen, Wang, Lei, Liang, Huanhuan. Crystal and solution structures of human protein-disulfide isomerase-like protein of the testis (PDILT) provide insight into its chaperone activity. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2018, 293(4): 1192-1202, http://dx.doi.org/10.1074/jbc.M117.797290.
[13] Zhang, Jianchao, Zhu, Qinyu, Wang, Xie, Yu, Jiaojiao, Chen, Xinxin, Wang, Jifeng, Wang, Xi, Xiao, Junyu, Wang, Chihchen, Wang, Lei. Secretory kinase Fam20C tunes endoplasmic reticulum redox state via phosphorylation of Ero1 alpha. EMBO JOURNAL[J]. 2018, 37(14): https://www.webofscience.com/wos/woscc/full-record/WOS:000438517100008.
[14] Yang, Kai, Li, DeFeng, Wang, Xie, Liang, Jinzhao, Sitia, Roberto, Wang, Chihchen, Wang, Xi. Crystal Structure of the ERp44-Peroxiredoxin 4 Complex Reveals the Molecular Mechanisms of Thiol-Mediated Protein Retention. STRUCTURE[J]. 2016, 24(10): 1755-1765, http://dx.doi.org/10.1016/j.str.2016.08.002.
[15] Niu, Yingbo, Zhang, Lihui, Yu, Jiaojiao, Wang, Chihchen, Wang, Lei. Novel Roles of the Non-catalytic Elements of Yeast Protein-disulfide Isomerase in Its Interplay with Endoplasmic Reticulum Oxidoreductin 1. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2016, 291(15): 8283-8294, http://dx.doi.org/10.1074/jbc.M115.694257.
[16] Wang, Lei, Wang, Xi, Wang, Chihchen. Protein disulfide-isomerase, a folding catalyst and a redox-regulated chaperone. FREE RADICAL BIOLOGY AND MEDICINE[J]. 2015, 83: 305-313, http://dx.doi.org/10.1016/j.freeradbiomed.2015.02.007.
[17] 王曦, 王磊, 陈畅, 王志珍. 未折叠蛋白响应:2014年拉斯克基础医学研究奖解读. 科学通报[J]. 2014, 59(36): 3533-3534, [18] Wang, Lei, Zhang, Lihui, Niu, Yingbo, Sitia, Roberto, Wang, Chihchen. Glutathione Peroxidase 7 Utilizes Hydrogen Peroxide Generated by Ero1 alpha to Promote Oxidative Protein Folding. ANTIOXIDANTS & REDOX SIGNALING[J]. 2014, 20(4): 545-556, http://dx.doi.org/10.1089/ars.2013.5236.
[19] Zhang, Lihui, Niu, Yingbo, Zhu, Li, Fang, Jingqi, Wang, Xie, Wang, Lei, Wang, Chihchen. Different Interaction Modes for Protein-disulfide Isomerase (PDI) as an Efficient Regulator and a Specific Substrate of Endoplasmic Reticulum Oxidoreductin-1 alpha (Ero1 alpha). JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2014, 289(45): 31188-31199, https://www.webofscience.com/wos/woscc/full-record/WOS:000344549900026.
[20] Zhu, Li, Yang, Kai, Wang, Xie, Wang, Xi, Wang, Chihchen. A Novel Reaction of Peroxiredoxin 4 towards Substrates in Oxidative Protein Folding. PLOS ONE[J]. 2014, 9(8): https://doaj.org/article/f3896079877b4ab29a17910a3dc878be.
[21] Yang, Shang, Wang, Xi, Cui, Lei, Ding, Xiang, Niu, Lili, Yang, Fuquan, Wang, Chao, Wang, Chihchen, Lou, Jizhong. Compact Conformations of Human Protein Disulfide Isomerase. PLOS ONE[J]. 2014, 9(8): https://doaj.org/article/018e58240135461486e86ccd2bc92afc.
[22] Wang, Chao, Li, Wei, Ren, Jinqi, Fang, Jingqi, Ke, Huimin, Gong, Weimin, Feng, Wei, Wang, Chihchen. Structural Insights into the Redox-Regulated Dynamic Conformations of Human Protein Disulfide Isomerase. ANTIOXIDANTS & REDOX SIGNALING[J]. 2013, 19(1): 44-53, http://www.irgrid.ac.cn/handle/1471x/756684.
[23] Wang, Xi, Wang, Likun, Wang, Xie, Sun, Fei, Wang, Chihchen. Structural insights into the peroxidase activity and inactivation of human peroxiredoxin 4. BIOCHEMICAL JOURNAL[J]. 2012, 441: 113-118, http://www.irgrid.ac.cn/handle/1471x/756941.
[24] Wang, Chao, Yu, Jiang, Huo, Lin, Wang, Lei, Feng, Wei, Wang, Chihchen. Human Protein-disulfide Isomerase Is a Redox-regulated Chaperone Activated by Oxidation of Domain a '. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2012, 287(2): 1139-1149, http://dx.doi.org/10.1074/jbc.M111.303149.
[25] Wang, Lei, Zhu, Li, Wang, Chihchen. The endoplasmic reticulum sulfhydryl oxidase Ero1 beta drives efficient oxidative protein folding with loose regulation. BIOCHEMICAL JOURNAL[J]. 2011, 434: 113-121, http://www.irgrid.ac.cn/handle/1471x/757444.
[26] Wang, Chihchen, Wang, ZhiXin, Zhang, Baoyuan, Li, Ming. Dr. Chen-lu Tsou: a tireless advocate for advancement in the standards of scientific research in China. PROTEIN & CELLnull. 2011, 2(6): 435-436, https://www.webofscience.com/wos/woscc/full-record/WOS:000310521600002.
[27] Nguyen, Van Dat, Saaranen, Mirva J, Karala, AnnaRiikka, Lappi, AnnaKaisa, Wang, Lei, Raykhel, Irina B, Alanen, Heli I, Salo, Kirsi E H, Wang, Chihchen, Ruddock, Lloyd W. Two Endoplasmic Reticulum PDI Peroxidases Increase the Efficiency of the Use of Peroxide during Disulfide Bond Formation. JOURNAL OF MOLECULAR BIOLOGY[J]. 2011, 406(3): 503-515, http://dx.doi.org/10.1016/j.jmb.2010.12.039.
[28] Wang, Chao, Chen, Sihong, Wang, Xi, Wang, Lei, Wallis, A Katrine, Freedman, Robert B, Wang, Chihchen. Plasticity of Human Protein Disulfide Isomerase EVIDENCE FOR MOBILITY AROUND THE X-LINKER REGION AND ITS FUNCTIONAL SIGNIFICANCE. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2010, 285(35): 26788-26797, http://dx.doi.org/10.1074/jbc.M110.107839.
[29] Du, Guiping, Liu, Xiang, Chen, Xinping, Song, Mei, Yan, Yan, Jiao, Renjie, Wang, Chihchen. Drosophila Histone Deacetylase 6 Protects Dopaminergic Neurons against alpha-Synuclein Toxicity by Promoting Inclusion Formation. MOLECULAR BIOLOGY OF THE CELL[J]. 2010, 21(13): 2128-2137, http://www.irgrid.ac.cn/handle/1471x/757040.
[30] Cheng, Han, Wang, Lei, Wang, Chihchen. Domain a' of protein disulfide isomerase plays key role in inhibiting alpha-synuclein fibril formation. CELL STRESS & CHAPERONES[J]. 2010, 15(4): 415-421, http://www.irgrid.ac.cn/handle/1471x/757108.
[31] Liu, Pengcheng, Meng, Xianglan, Qu, Peng, Zhao, Xin Sheng, Wang, Chihchen. Subdomain-Specific Collapse of Denatured Staphylococcal Nuclease Revealed by Single Molecule Fluorescence Resonance Energy Transfer Measurements. JOURNAL OF PHYSICAL CHEMISTRY B[J]. 2009, 113(35): 12030-12036, http://www.irgrid.ac.cn/handle/1471x/757832.
[32] Li, Heng, Ke, Huimin, Ren, Guoping, Qiu, Xianggang, Weng, YuXiang, Wang, ChihChen. Thermal-Induced Dissociation and Unfolding of Homodimeric DsbC Revealed by Temperature-Jump Time-Resolved Infrared Spectra. BIOPHYSICAL JOURNAL[J]. 2009, 97(10): 2811-2819, http://www.irgrid.ac.cn/handle/1471x/757953.
[33] Wang, Lei, Li, Shengjian, Sidhu, Ateesh, Zhu, Li, Liang, Yi, Freedman, Robert B, Wang, Chihchen. Reconstitution of Human Ero1-L alpha/Protein-Disulfide Isomerase Oxidative Folding Pathway in Vitro POSITION-DEPENDENT DIFFERENCES IN ROLE BETWEEN THE a AND a ' DOMAINS OF PROTEIN-DISULFIDE ISOMERASE. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2009, 284(1): 199-206, http://www.irgrid.ac.cn/handle/1471x/757946.
[34] Wang, Likun, Wang, Lei, Vavassori, Stefano, Li, Shengjian, Ke, Huimin, Anelli, Tiziana, Degano, Massimo, Ronzoni, Riccardo, Sitia, Roberto, Sun, Fei, Wang, Chihchen. Crystal structure of human ERp44 shows a dynamic functional modulation by its carboxy-terminal tail. EMBO REPORTS[J]. 2008, 9(7): 642-647, http://www.irgrid.ac.cn/handle/1471x/757645.
[35] Ren, Guoping, Wang, Xi, Hao, Shufeng, Hu, Hongyu, Wang, Chihchen. Translocation of alpha-synuclein expressed in Escherichia coli. JOURNAL OF BACTERIOLOGY[J]. 2007, 189(7): 2777-2786, https://www.webofscience.com/wos/woscc/full-record/WOS:000245842000023.
[36] He, RongQiao, Chen, Lan, Perrett, Sarah, Wang, Chihchen. A combination of modifying factors induces protein misfolding in vivo. BIOPHYSICAL JOURNALnull. 2007, 221A-221A, https://www.webofscience.com/wos/woscc/full-record/WOS:000243972401232.
[37] He RongQiao, Chen Lan, Sarah Perrett, Wang ChihChen. Hypothesis: a combination of modifying factors induces misfolding and dysfunction of selected proteins in vivo. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2006, 33(10): 940-941, https://www.webofscience.com/wos/woscc/full-record/WOS:000241569200005.
[38] 王志珍. The C-terminal (331-376) sequence of Escherichia coli DnaJ is essential for dimerization and chaperone activity: A small angle X-ray scattering study in solution. J Biol Chem. 2005, 280: 22761-22768, [39] 王志珍. 蛋白质折叠和分子伴侣. 生物学通报[J]. 2004, 39(5): 1-5,6, http://lib.cqvip.com/Qikan/Article/Detail?id=9725403.
[40] 王志珍. 一个科技里程碑:分子生物学的中心法则. 生理科学进展[J]. 2003, 34(2): 101-103, http://lib.cqvip.com/Qikan/Article/Detail?id=8000786.
[41] 李剑, 王志珍. 分子伴侣、蛋白质聚集和大分子拥挤环境对蛋白质折叠的影响. 中国科学院研究生院学报. 2002, 19(2): 215-218, http://lib.cqvip.com/Qikan/Article/Detail?id=6480689.
[42] 王志珍, 李剑. 细胞内的大分子拥挤环境. 生物化学与生物物理进展. 2001, 28(6): 788-, http://lib.cqvip.com/Qikan/Article/Detail?id=5767798.
[43] 王志珍. Effects of Macromolecular Crowding on the Refolding of Glucose-6-Phosphate Dehydrogenase and Protein Disulfide Isomerase. J Biol Chem. 2001, 276: 34396-34401, [44] 王志珍. Disulfide-dependent folding and export of Escherichia coli DsbC. J Biol Chem. 2001, 276: 1146-1151, [45] 王志珍. The N-terminal sequence of (residues 1-65) is essential for dimerization, activity and peptide binding of Escherichia coli DsbC. J Biol Chem. 2000, 275: 22743-22749, [46] 王志珍. "Half of the sites" binding of D-glyceraldehyde-3-phosphate dehydrogenase folding intermediate with GroEL. J Biol Chem. 1999, 274: 10790-10794, [47] 王志珍. Chaperone activity of DsbC. J Biol Chem. 1999, 274: 19601-19604, [48] 王志珍, 邹承鲁. 后基因组——蛋白质组研究. 生物化学与生物物理学报:英文版. 1998, 30(6): 533-539, http://lib.cqvip.com/Qikan/Article/Detail?id=3254813.
[49] 王志珍. A mutant truncated protein disulfide isomerase with no chaperone activity. J Biol Chem. 1997, 272: 27572-27576, [50] 高音, 王志珍. 用质粒pCU18在大肠杆菌中表达人蛋白质二硫键异构酶. 生物化学杂志[J]. 1996, 12(2): 237-, [51] 邹承鲁, 王志珍. 立足国内 走向世界:从吴宪教授六十四年前一篇论文的重新发表谈起. 生理科学进展[J]. 1996, 27(1): 5-, [52] 王志珍. Independence of the chaperone activity of protein disulfide isomerase from its thioredoxin-like active site. J Biol Chem. 1995, 270: 17078-17080, [53] 王志珍. 从近三届国际生物物理学大会看当代生物物理学发展的特点和前沿. 生物物理学报[J]. 1995, 11(1): 110-, [54] 王志珍. Chaperone-like activity of protein disulfide isomerase in the refolding of a protein with no disulfide bounds. J Biol Chem. 1994, 269: 24550-24552, [55] 梁栋材, 王志珍. Hodgkin教授与中国的胰岛素晶体学研究. 化学通报[J]. 1994, 60-, http://lib.cqvip.com/Qikan/Article/Detail?id=1384535.
[56] 谢贵福, 王志珍, 李淑莲. 巯基—二硫键交换在胰岛素与其受体结合中的作用. 中国科学:B辑[J]. 1991, 724-, http://lib.cqvip.com/Qikan/Article/Detail?id=699924.
[57] 王志珍. Insulin induced internalization of the insulin receptor in the isolated rat adipose cells: detection of the internalized 138-kilodalton receptor subunit using a photoaffinity I125-insulin. J Biol Chem. 1983, 258: 5129-5134, 

科研活动

   
科研项目
( 1 ) 蛋白质折叠中折叠酶和分子伴侣的结构与功能研究, 主持, 国家级, 2011-01--2015-08
( 2 ) 蛋白质氧化折叠与氧化还原修饰, 参与, 国家级, 2012-01--2016-08
( 3 ) Peroxiredoxin 4介导蛋白质氧化折叠通路的分子机制及结构基础, 参与, 国家级, 2014-01--2017-12
( 4 ) 内质网中蛋白质氧化折叠及其调控的新机制, 参与, 国家级, 2014-01--2017-12
( 5 ) Ero1α翻译后修饰介导的内质网氧化应激及抗氧化应答, 参与, 国家级, 2016-01--2019-12
( 6 ) 生物膜相关蛋白质机器的动态变化、结构与功能, 参与, 国家级, 2016-07--2021-06
( 7 ) 基于糖尿病发生进程关键信号通路的分子靶标的发现与确认, 参与, 部委级, 2016-01--2017-12

指导学生

已指导学生

史渊源  博士研究生  071010-生物化学与分子生物学  

张念湘  博士研究生  071016-分子生物学  

张森  博士研究生  071010-生物化学与分子生物学  

李剑  博士研究生  071010-生物化学与分子生物学  

黄春娟  博士研究生  071010-生物化学与分子生物学  

孙秀霞  博士研究生  071016-生物化学与分子生物学  

尹金虎  博士研究生  071016-分子生物学  

任国平  博士研究生  071010-生物化学与分子生物学  

李升建  博士研究生  071010-生物化学与分子生物学  

唐炜  博士研究生  071010-生物化学与分子生物学  

李文奇  硕士研究生  071010-生物化学与分子生物学  

刘鹏程  博士研究生  071010-生物化学与分子生物学  

刘翔  硕士研究生  071010-生物化学与分子生物学  

柯慧敏  博士研究生  071010-生物化学与分子生物学  

王曦  博士研究生  071010-生物化学与分子生物学  

程汉  博士研究生  071010-生物化学与分子生物学  

陶薇  硕士研究生  071010-生物化学与分子生物学  

朱际  硕士研究生  071010-生物化学与分子生物学  

王磊  博士研究生  071010-生物化学与分子生物学  

王立堃  博士研究生  071010-生物化学与分子生物学  

梁羽  硕士研究生  071009-细胞生物学  

王超  博士研究生  071010-生物化学与分子生物学  

孙悦  硕士研究生  071010-生物化学与分子生物学  

朱丽  博士研究生  071010-生物化学与分子生物学  

杨尚  硕士研究生  071010-生物化学与分子生物学  

张立慧  博士研究生  071010-生物化学与分子生物学  

牛英波  博士研究生  071010-生物化学与分子生物学  

现指导学生

梁今朝  硕士研究生  071010-生物化学与分子生物学  

于娇娇  硕士研究生  071010-生物化学与分子生物学  

吴逊  博士研究生  071010-生物化学与分子生物学  

杨锴  博士研究生  071010-生物化学与分子生物学  

房靖淇  博士研究生  071010-生物化学与分子生物学  

李涛  硕士研究生  071010-生物化学与分子生物学  

陈欣欣  硕士研究生  071009-细胞生物学  

张建超  博士研究生  071010-生物化学与分子生物学  

张旖旎  博士研究生  071010-生物化学与分子生物学