基本信息
王可品 男 博导 中国科学院广州生物医药与健康研究院
电子邮件: wang_kepin@gibh.ac.cn
通信地址: 广州市黄埔区开源大道190号
邮政编码:
电子邮件: wang_kepin@gibh.ac.cn
通信地址: 广州市黄埔区开源大道190号
邮政编码:
招生信息
招生专业
071008-发育生物学071010-生物化学与分子生物学
招生方向
基因修饰大动物模型构建与应用新型基因编辑技术开发与优化
教育背景
2011-09--2016-07 中国科学院大学 理学博士2007-09--2011-07 安徽大学 工学学士
工作经历
工作简历
2022-12~现在, 中国科学院广州生物医药与健康研究院, 研究员2018-07~2022-12,中国科学院广州生物医药与健康研究院, 副研究员2018-01~2018-06,中国科学院广州生物医药与健康研究院, 副研究员(试聘)2016-07~2017-12,中国科学院广州生物医药与健康研究院, 助理研究员
社会兼职
2020-09-01-2024-08-31,中国科学院青年创新促进会广州分会, 第三届、第四届委员会委员
2018-01-01-2022-12-31,广东省遗传学会, 理事,副秘书长
2018-01-01-2022-12-31,广东省遗传学会, 理事,副秘书长
专利与奖励
奖励信息
(1) 中国科学院广州分院2021-2022年度优秀青年科技工作者, 院级, 2023
专利成果
[1] 赖良学, 谢精科, 王可品, 庄镇鹏, 苟士学, 张全军. 一种定量调控基因翻译的方法及其应用. 202310097886.5, 2023-02-10.[2] 赖良学, 王可品, 石惠, 金琴, 陈方兵. 一种可逆基因敲除系统及其应用. 202210730142.8, 2022-08-02.[3] 赖良学, 王可品, 金琴. 一种构建可通过药物诱导Cas9蛋白表达的模型猪的方法. 202210822307.4, 2022-07-14.[4] 赖良学, 王可品, 梁艳慧, 谢精科. 一种融合蛋白及含有其的由双脱氨酶介导的碱基编辑系统和应用. 202210193759.0, 2022-03-24.[5] 赖良学, 王可品, 梁艳慧, 谢精科. 一种融合蛋白及含有其的由双脱氨酶介导的碱基编辑系统和应用. CN: CN114480348A, 2022-05-13.[6] 赖良学, 王可品, 金琴. 一种在猪上诱导高效表达外源基因的方法. CN: CN113817773A, 2021-12-21.[7] 赖良学, 王可品, 葛维凯, 刘琪帅, 谢精科. 一种重度免疫缺陷猪模型及其构建方法和应用. CN: CN109777834A, 2019-05-21.[8] 王可品, 石惠, 赖良学, 李磊, 金琴. 外切酶编辑器及基因编辑方法. 202410618459.1, 2024-05-17.[9] 赖良学, 王可品, 石惠, 金琴, 穆双双, 李倩如. 异源二聚体型基因编辑器及其制法和应用. 202410507798.2, 2024-04-25.[10] 赖良学, 王可品, 石惠, 金琴, 陈方兵. 一种可逆基因敲除系统及其应用. CN: CN117305358A, 2023-12-29.
出版信息
发表论文
[1] Mu, Shuangshuang, Chen, Huangyao, Li, Qianru, Gou, Shixue, Liu, Xiaoyi, Wang, Junwei, Zheng, Wei, Chen, Menglong, Jin, Qin, Lai, Liangxue, Wang, Kepin, Shi, Hui. Enhancing prime editor flexibility with coiled-coil heterodimers. Genome Biology[J]. 2024, 第 11 作者 通讯作者 25: 108, http://dx.doi.org/10.1186/s13059-024-03257-z.[2] Shi, Hui, Li, Lei, Mu, Shuangshuang, Gou, Shixue, Liu, Xiaoyi, Chen, Fangbing, Chen, Menglong, Jin, Qin, Lai, Liangxue, Wang, Kepin. Exonuclease editor promotes precision of gene editing in mammalian cells. BMC Biology[J]. 2024, 第 10 作者 通讯作者 22: 119, [3] Ge, Weikai, Zhao, Xiaozhu, Gou, Shixue, Jin, Qin, Chen, Fangbing, Ouyang, Zhen, Lai, Chengdan, Cui, Tao, Mai, Baoyi, Lu, Sijia, Zhong, Kexin, Liang, Yanhui, Chen, Tao, Wu, Han, Li, Nan, Ye, Yinghua, Lai, Liangxue, Wang, Kepin. Evaluation of guide-free Cas9-induced genomic damage and transcriptome changes in pig embryos. Molecular Therapy - Nucleic Acids[J]. 2023, 第 18 作者 通讯作者 34: 102035, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10551558/.[4] Xie, Jingke, Zhuang, Zhenpeng, Gou, Shixue, Zhang, Quanjun, Wang, Xia, Lan, Ting, Lian, Meng, Li, Nan, Liang, Yanhui, Ouyang, Zhen, Ye, Yinghua, Wu, Han, Lai, Liangxue, Wang, Kepin. Precise genome editing of the Kozak sequence enables bidirectional and quantitative modulation of protein translation to anticipated levels without affecting transcription. Nucleic Acids Research[J]. 2023, 第 14 作者 通讯作者 [5] Jin, Qin, Liu, Xiaoyi, Zhuang, Zhenpeng, Huang, Jiayuan, Gou, Shixue, Shi, Hui, Zhao, Yu, Ouyang, Zhen, Liu, Zhaoming, Li, Lei, Mao, Junjie, Ge, Weikai, Chen, Fangbing, Yu, Manya, Guan, Yezhi, Ye, Yinghua, Tang, Chengcheng, Huang, Ren, Wang, Kepin, Lai, Liangxue. Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation. Genome Biology[J]. 2023, 第 19 作者 通讯作者 24: 8, http://dx.doi.org/10.1186/s13059-023-02851-x.[6] Jin, Qin, Shi, Hui, Liu, Xiaoyi, Ju, Jialu, Wang, Kepin, Lai, Liangxue. Genome editing pig models with elements for controllable gene expression. Animal Research and One Health[J]. 2023, 第 5 作者 通讯作者 1(2): 242-258, [7] Wang, Xunlei, Jin, Qin, Xiao, Wenwen, Fang, Puxian, Lai, Liangxue, Xiao, Shaobo, Wang, Kepin, Fang, Liurong. Genome-Wide CRISPR/Cas9 Screen Reveals a Role for SLC35A1 in the Adsorption of Porcine Deltacoronavirus. Journal of Virology[J]. 2022, 第 7 作者 通讯作者 96(24): e0162622, [8] Liang, Yanhui, Xie, Jingke, Zhang, Quanjun, Wang, Xiaomin, Gou, Shixue, Lin, Lihui, Chen, Tao, Ge, Weikai, Zhuang, Zhenpeng, Lian, Meng, Chen, Fangbing, Li, Nan, Ouyang, Zhen, Lai, Chengdan, Liu, Xiaoyi, Li, Lei, Ye, Yinghua, Wu, Han, Wang, Kepin, Lai, Liangxue. AGBE: a dual deaminase-mediated base editor by fusing CGBE with ABE for creating a saturated mutant population with multiple editing patterns. Nucleic Acids Research[J]. 2022, 第 19 作者 通讯作者 50(9): 5384-5399, http://dx.doi.org/10.1093/nar/gkac353.[9] Jin, Qin, Yang, Xiaoyu, Gou, Shixue, Liu, Xiaoyi, Zhuang, Zhenpeng, Liang, Yanhui, Shi, Hui, Huang, Jiayuan, Wu, Han, Zhao, Yu, Ouyang, Zhen, Zhang, Quanjun, Liu, Zhaoming, Chen, Fangbing, Ge, Weikai, Xie, Jingke, Li, Nan, Lai, Chengdan, Zhao, Xiaozhu, Wang, Jiaowei, Lian, Meng, Li, Lei, Quan, Longquan, Ye, Yinghua, Lai, Liangxue, Wang, Kepin. Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression. Science China-Life Sciences[J]. 2022, 第 26 作者 通讯作者 65(11): 2269-2286, http://dx.doi.org/10.1007/s11427-021-2088-1.[10] Li, Nan, Gou, Shixue, Wang, Jiaowei, Zhang, Quanjun, Huang, Xingyun, Xie, Jingke, Li, Li, Jin, Qin, Ouyang, Zhen, Chen, Fangbing, Ge, Weikai, Shi, Hui, Liang, Yanhui, Zhuang, Zhenpeng, Zhao, Xiaozhu, Lian, Meng, Ye, Yinghua, Quan, Longquan, Wu, Han, Lai, Liangxue, Wang, Kepin. CRISPR/Cas9-Mediated Gene Correction in Newborn Rabbits with Hereditary Tyrosinemia Type I. Molecular Therapy[J]. 2021, 第 21 作者 通讯作者 29(3): 1001-1015, http://dx.doi.org/10.1016/j.ymthe.2020.11.023.[11] Meng Lian, Fangbing Chen, Xingyun Huang, Xiaozhu Zhao, Shixue Gou, Nan Li, Qin Jin, Hui Shi, Yanhui Liang, Jingke Xie, Weikai Ge, Zhenpeng Zhuang, Jiaowei Wang, Yinghua Ye, Yi Yang, Kepin Wang, Liangxue Lai, Han Wu. Improving Cpf1-mediated base editing system by combining dCas9/ dead sgRNA with human APOBEC3A variants. Journal of Genetics and Genomics[J]. 2021, 第 16 作者 通讯作者 48(1): 92-95, http://dx.doi.org/10.1016/j.jgg.2020.07.010.[12] Li, Wenjing, Shi, Lei, Zhuang, Zhenpeng, Wu, Han, Lian, Meng, Chen, Yihui, Li, Lei, Ge, Weikai, Jin, Qin, Zhang, Quanjun, Zhao, Yu, Liu, Zhaoming, Ouyang, Zhen, Ye, Yinghua, Li, Yingying, Wang, Hai, Liao, Yuan, Quan, Longquan, Xiao, Lei, Lai, Liangxue, Meng, Guangxun, Wang, Kepin. Engineered Pigs Carrying a Gain-of-Function NLRP3 Homozygous Mutation Can Survive to Adulthood and Accurately Recapitulate Human Systemic Spontaneous Inflammatory Responses. Journal of Immunology[J]. 2020, 第 22 作者 通讯作者 205(9): 2532-2544, https://www.webofscience.com/wos/woscc/full-record/WOS:000581921400019.[13] Xie, Jingke, Huang, Xingyun, Wang, Xia, Gou, Shixue, Liang, Yanhui, Chen, Fangbing, Li, Nan, Ouyang, Zhen, Zhang, Quanjun, Ge, Weikai, Jin, Qin, Shi, Hui, Zhuang, Zhenpeng, Zhao, Xiaozhu, Lian, Meng, Wang, Jiaowei, Ye, Yinghua, Quan, Longquan, Wu, Han, Wang, Kepin, Lai, Liangxue. ACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems. BMC Biology[J]. 2020, 第 20 作者 通讯作者 18: 131, http://dx.doi.org/10.1186/s12915-020-00866-5.[14] Jingke Xie, Weikai Ge, Nan Li, Qishuai Liu, Fangbing Chen, Xiaoyu Yang, Xingyun Huang, Zhen Ouyang, Quanjun Zhang, Yu Zhao, Zhaoming Liu, Shixue Gou, Han Wu, Chengdan Lai, Nana Fan, Qin Jin, Hui Shi, Yanhui Liang, Ting Lan, Longquan Quan, Xiaoping Li, Kepin Wang, Liangxue Lai. Efficient base editing for multiple genes and loci in pigs using base editors. Nature Communications[J]. 2019, 第 22 作者 通讯作者 10: 2852, [15] Ruan, Degong, Peng, Jiangyun, Wang, Xiaoshan, Ouyang, Zhen, Zou, Qingjian, Yang, Yi, Chen, Fangbing, Ge, Weikai, Wu, Han, Liu, Zhaoming, Zhao, Yu, Zhao, Bentian, Zhang, Quanjun, Lai, Chengdan, Fan, Nana, Zhou, Zhiwei, Liu, Qishuai, Li, Nan, Jin, Qin, Shi, Hui, Xie, Jingke, Song, Hong, Yang, Xiaoyu, Chen, Jiekai, Wang, Kepin, Li, Xiaoping, Lai, Liangxue. XIST Derepression in Active X Chromosome Hinders Pig Somatic Cell Nuclear Transfer. Stem Cell Reports[J]. 2018, 第 25 作者 通讯作者 10(2): 494-508, http://dx.doi.org/10.1016/j.stemcr.2017.12.015.[16] Wu, Han, Liu, Qishuai, Shi, Hui, Xie, Jingke, Zhang, Quanjun, Ouyang, Zhen, Li, Nan, Yang, Yi, Liu, Zhaoming, Zhao, Yu, Lai, Chengdan, Ruan, Degong, Peng, Jiangyun, Ge, Weikai, Chen, Fangbing, Fan, Nana, Jin, Qin, Liang, Yanhui, Lan, Ting, Yang, Xiaoyu, Wang, Xiaoshan, Lei, Zhiyong, Doevendans, Pieter A, Sluijter, Joost P G, Wang, Kepin, Li, Xiaoping, Lai, Liangxue. Engineering CRISPR/Cpf1 with tRNA promotes genome editing capability in mammalian systems. Cellular and Molecular Life Sciences[J]. 2018, 第 25 作者 通讯作者 75(19): 3593-3607, http://www.corc.org.cn/handle/1471x/2202255.[17] Wang, Kepin, Jin, Qin, Ruan, Degong, Yang, Yi, Liu, Qishuai, Wu, Han, Zhou, Zhiwei, Ouyang, Zhen, Liu, Zhaoming, Zhao, Yu, Zhao, Bentian, Zhang, Quanjun, Peng, Jiangyun, Lai, Chengdan, Fan, Nana, Liang, Yanhui, Lan, Ting, Li, Nan, Wang, Xiaoshan, Wang, Xinlu, Fan, Yong, Doevendans, Pieter A, Sluijter, Joost P G, Liu, Pentao, Li, Xiaoping, Lai, Liangxue. Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing. Genome Research[J]. 2017, 第 1 作者27(12): 2061-2071, https://www.webofscience.com/wos/woscc/full-record/WOS:000417047600009.[18] Yang, Yi, Wang, Kepin, Wu, Han, Jin, Qin, Ruan, Degong, Ouyang, Zhen, Zhao, Bentian, Liu, Zhaoming, Zhao, Yu, Zhang, Quanjun, Fan, Nana, Liu, Qishuai, Guo, Shimei, Bu, Lei, Fan, Yong, Sun, Xiaofang, Li, Xiaoping, Lai, Liangxue. Genetically humanized pigs exclusively expressing human insulin are generated through custom endonuclease-mediated seamless engineering. Journal of Molecular Cell Biology[J]. 2016, 第 2 作者8(2): 174-177, http://ir.gibh.ac.cn/handle/344009/868.
科研活动
科研项目
( 1 ) 利用单碱基编辑工具构建儿童早衰症猪模型, 负责人, 国家任务, 2020-01--2022-12( 2 ) 利用CRISPR/Cpf1对杜氏肌营养不良症(DMD)猪模型进行基因治疗研究, 负责人, 国家任务, 2018-01--2020-12( 3 ) 条件性基因敲除与恢复系统的建立及其应用, 负责人, 地方任务, 2020-04--2023-03( 4 ) 基于条件性表达Cas9系统构建谱系示踪工具猪模型, 负责人, 国家任务, 2022-01--2025-12( 5 ) β地中海贫血五指山猪模型构建及其基因治疗研究, 负责人, 地方任务, 2021-09--2023-09( 6 ) 基因修饰猪模型资源库建设, 负责人, 中国科学院计划, 2020-01--2021-12( 7 ) 代谢紊乱条件性敲除猪模型的建立及发病机理研究, 负责人, 国家任务, 2022-01--2026-12( 8 ) 心肾移植猪源供体标准化体系研究, 负责人, 国家任务, 2024-01--2028-12
参与会议
(1)嵌有可调控元件工具猪模型培育与应用 2023中国研究型医院学会医学动物实验专家委员会第二届临床医学研究与医学动物实验大会 2023-05-26(2)动物基因编辑技术及其应用 2023世界种业论坛暨世界畜禽产业博览会 2023-03-18(3)可诱导外源基因表达猪模型培育与应用 广东省实验动物学会2022年度学术年会 2023-02-24
指导学生
现指导学生
穆双双 硕士研究生 071008-发育生物学
李紫琪 硕士研究生 086000-生物与医药