基本信息
唐卓  男  博导  中国科学院成都生物研究所
电子邮件: tangzhuo@cib.ac.cn
通信地址: 成都市人民南路四段九号
邮政编码: 610041

研究领域


一、化学生物学

a. 体外筛选功性生物大分子

b. 基因检测方法和核酸探针的设计开发

c.多肽的展示技术以及在药物筛选中的应用


二、有机化学与核酸化学

a. 生物启发的有机合成反应

b. DNA催化的不对称反应反应

c. 改性核苷与DNA合成


教育背景

2003-07--2006-01   中科院成都有机化学研究所   博士
2000-09--2003-07   四川大学与成都生物研究所联合培养   硕士
学历
研究生

学位
博士

工作经历

   
工作简历
2009-06~现在, 中国科学院成都生物所, 研究员
2008-03~2009-06,加拿大麦克马斯特大学, 博士后
2006-01~2008-03,德国康斯坦茨大学, 博士后洪堡学者

专利与奖励

   
奖励信息
(1) 四川省科学技术进步奖, 三等奖, 省级, 2021
(2) 四川省五一劳动奖章, 省级, 2015
(3) 第十八届“四川青年五四奖章, , 省级, 2015
(4) 中国科学院先进工作者, 院级, 2015
(5) 国家自然科学二等奖, 二等奖, 国家级, 2013
(6) 万人计划, , 部委级, 2013
(7) 四川省****, , 省级, 2012
(8) 获引进国外杰出人才择优支持, , 院级, 2010
(9) ****, , 院级, 2009
(10) 全国百篇优秀博士论文奖, , 国家级, 2008
(11) 洪堡奖学金, , 国家级, 2006
(12) 中科院院长特等奖学金, 特等奖, 院级, 2005
专利成果
( 1 ) 一种用于检测小鼠肝炎病毒的超快速实时荧光VPCR试剂盒和方法, 发明专利, [[[", 专利号: [[[CN111690774A]]]

( 2 ) 一种手性溴代1,4-二氢吡啶类化合物的制备方法, 发明专利, 2022, 第 1 作者, 专利号: 202210068642.X

( 3 ) 一种手性氮取代四氢喹喔啉的制备方法, 发明专利, 2022, 第 1 作者, 专利号: 202210068649.1

( 4 ) 靶向降解酪氨酸酶的PROTAC及其应用, 发明专利, 2021, 第 1 作者, 专利号: 202111043833.2

( 5 ) 一种等温解开双链DNA及制备单链DNA的方法, 专利授权, 2020, 第 1 作者, 专利号: CN106701738B

( 6 ) 一种荧光探针, 发明专利, 2020, 第 1 作者, 专利号: CN110724729A

( 7 ) 基于重组酶的环介导扩增方法, 发明专利, 2020, 第 1 作者, 专利号: CN110684826A

( 8 ) 基于LAMP技术的转基因抗除草剂外源基因的检测方法及试剂盒, 发明专利, 2019, 第 1 作者, 专利号: CN110592193A

( 9 ) 基于LAMP技术的转基因玉米Bt176相关基因检测方法及试剂盒, 发明专利, 2019, 第 1 作者, 专利号: CN110564822A

( 10 ) 基于LAMP技术的动物源性成分快速检测方法, 发明专利, 2019, 第 1 作者, 专利号: CN110257530A

( 11 ) 一种单核苷酸多态性的等温分型方法, 发明专利, 2019, 第 1 作者, 专利号: CN110172500A

( 12 ) 一种体内筛选的剪切RNA的锤头状核酶, 发明专利, 2019, 第 1 作者, 专利号: CN109609505A

( 13 ) 一种寡核苷酸探针, 专利授权, 2019, 第 1 作者, 专利号: CN105802963B

( 14 ) 一种快速扩增核酸的方法, 发明专利, 2018, 第 1 作者, 专利号: CN108642147A

( 15 ) 一种基于DNAzyme探针的乙肝病毒检测方法, 发明专利, 2018, 第 2 作者, 专利号: CN103952497B

( 16 ) 一种冬虫夏草的真伪鉴别方法, 发明专利, 2018, 第 1 作者, 专利号: CN108130365A

( 17 ) 核黄素及其衍生物作为癌症治疗靶向药物及其应用, 发明专利, 2017, 第 1 作者, 专利号: CN107468694A

( 18 ) 一种广谱性的癌症治疗基因靶点及其应用, 发明专利, 2017, 第 1 作者, 专利号: CN107384916A

( 19 ) 5’位改性嘌呤霉素化合物及其制备方法和用途, 发明专利, 2013, 第 1 作者, 专利号: CN103382212A

( 20 ) (S,S)-八氢-6H-吡咯并3,4-b吡啶的制备方法, 发明专利, 2013, 第 1 作者, 专利号: CN103030638A

( 21 ) 一种体外筛选多肽的方法, 发明专利, 2013, 第 1 作者, 专利号: CN103382579A

( 22 ) 中成药中生物类伪品DNA的PCR检测方法, 发明专利, 2013, 第 1 作者, 专利号: CN103131781A

( 23 ) 一种检测单核苷酸多态性的方法, 发明专利, 2013, 第 1 作者, 专利号: CN103103283A

( 24 ) 一种RNA的等温检测方法, 发明专利, 2013, 第 1 作者, 专利号: CN103014148A

( 25 ) 一种剪切DNA的DNA酶, 发明专利, 2013, 第 1 作者, 专利号: CN103013953A

( 26 ) 一种寡核苷酸探针以及用其对靶分子进行检测的方法, 发明专利, 2012, 第 1 作者, 专利号: CN102827836A

( 27 ) 中成药中人参DNA的PCR检测方法, 发明专利, 2012, 第 1 作者, 专利号: CN102827940A

( 28 ) 转BAR基因农作物的可视化检测方法, 发明专利, 2012, 第 1 作者, 专利号: CN102787175A

( 29 ) 一种PCR检测核酸的方法, 发明专利, 2011, 第 1 作者, 专利号: CN102071251A

出版信息

   
发表论文
(1) Preparation and Application of α‑Imino Ketones through One-Pot Tandem Reactions Based on Heyns Rearrangement, Org. Lett., 2021, 通讯作者
(2) Regioselective, Diastereoselective, and Enantioselective One-Pot Tandem Reaction Based on an in Situ Formed Reductant: Preparation of 2,3-Disubstituted 1,5-Benzodiazepine, JOURNAL OF ORGANIC CHEMISTRY, 2021, 通讯作者
(3) Combining Protein and RNA Quantification to Evaluate Promoter Activity by Using Dual-color Fluorescent Reporting Systems, Bioscience Reports, 2021, 通讯作者
(4) Catalytic asymmetric synthesis of N-substituted tetrahydroquinoxalines via regioselective Heyns rearrangement and stereoselective transfer hydrogenation in one pot, CHEMICAL SCIENCE, 2021, 通讯作者
(5) Biomimetic alloxan-catalyzed intramolecular redox reaction with O2: One-pot atom-economic synthesis of sulfinyl-functionalized benzimidazoles, TETRAHEDRON LETTERS, 2021, 通讯作者
(6) Sequence-specific and multiplex detection of COVID-19 virus (SARS-CoV-2) using proofreading enzyme-mediated probe cleavage coupled with isothermal amplification, Biosensors and Bioelectronics, 2021, 通讯作者
(7) Design, synthesis and biological evaluation of tyrosinase-targeting PROTACs, EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2021, 通讯作者
(8) An organocatalytic asymmetric Friedel–Crafts reaction of 2-substituted indoles with aldehydes: enantioselective synthesis of a-hydroxyl ketones by low loading of chiral phosphoric acid, ChemComm, 2020, 通讯作者
(9) Intracellular Selection of Theophylline-Sensitive Hammerhead Aptazyme, Molecular Therapy - Nucleic Acids, 2020, 通讯作者
(10) Alloxan-Catalyzed Biomimetic Oxidations with Hydrogen Peroxide or Molecular Oxygen, ACS CATALYSIS, 2020, 通讯作者
(11) Ion-induced PCR strategy for mercury detection, ELECTROPHORESIS, 2020, 通讯作者
(12) Recombinase assisted loop-mediated isothermal DNA amplification, ANALYST, 2020, 通讯作者
(13) RNA适配体作为类抗性基因的可行性, A feasibility study into ribonucleic acid aptamer being antibiotic resistance gene, 微生物学通报, 2020, 通讯作者
(14) Frame-shifted proteins of a given gene retain the same function, NUCLEIC ACIDS RESEARCH, 2020, 通讯作者
(15) Pictet-Spengler reaction based onin situgenerated alpha-amino iminium ions through the Heyns rearrangement, ORGANIC CHEMISTRY FRONTIERS, 2020, 通讯作者
(16) Chiral Bronsted-Acid-Catalyzed Asymmetric Oxidation of Sulfenamide by Using H2O2: A Versatile Access to Sulfinamide and Sulfoxide with High Enantioselectivity, ACS CATALYSIS, 2019, 通讯作者
(17) 基于环介导等温扩增法可视化检测玉米转基因成分, Colorimetric detection of genetically modified organisms(GMOs)based on loop-mediated isothermal amplification(LAMP), 应用与环境生物学报, 2019, 通讯作者
(18) Preparation of Bicyclic Ketal Skeletons with Aldehyde and alpha-Ketone Acid through Cascade Friedel-Crafts Reaction and Stereoselective Acetalization in One Pot, SYNLETT, 2019, 通讯作者
(19) Intracellular selection of trans -cleaving hammerhead ribozymes, NUCLEIC ACIDS RESEARCH, 2019, 通讯作者
(20) Polymerase Chain Reaction using "V" Shape Thermal Cycling Program, THERANOSTICS, 2019, 通讯作者
(21) 基于LAMP的病原菌一步等温可视化检测, One-step isothermal visual detection of pathogens based on LAMP, 应用与环境生物学报, 2019, 通讯作者
(22) From Electrophoresis Detection to Visual Judgment: New Application of SYBR Green I in the Authentication of the Traditional Medicine Rhizoma Paridis, INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY, 2019, 第 6 作者
(23) Colorimetric Detection of Horse Meat Based on Loop-Mediated Isothermal Amplification (LAMP), FOOD ANALYTICAL METHODS, 2019, 通讯作者
(24) Genetically Encoded Residue-Selective Photo-Crosslinker to Capture Protein-Protein Interactions in Living Cells, CHEM, 2019, 通讯作者
(25) A general fluorescent light-up probe for staining and quantifying protein, ROYAL SOCIETY OPEN SCIENCE, 2019, 通讯作者
(26) 一种利用活性炭提取中药材DNA的方法, A simple method for extracting DNA from Chinese herbal medicine with activated carbon, 天然产物研究与开发, 2019, 通讯作者
(27) One-step colorimetric genotyping of single nucleotide polymorphism using probe-enhanced loop-mediated isothermal amplification (PE-LAMP), THERANOSTICS, 2019, 通讯作者
(28) Solvent-free synthesis of -amino ketones from -hydroxyl ketones via a novel tandem reaction sequence based on heyns rearrangement, SYNLETT, 2019, 通讯作者
(29) A high-throughput in vivo selection method for luciferase variants, SENSORS AND ACTUATORS B-CHEMICAL, 2018, 通讯作者
(30) Bioinspired Catalysis: Self-Assembly of a Protein and DNA as a Catalyst for the Aldol Reaction in Aqueous Media, SYNLETT, 2018, 通讯作者
(31) PI(4,5)P2 determines the threshold of mechanical force–induced B cell activation, JOURNAL OF CELL BIOLOGY, 2018, 第 13 作者
(32) Colorimetric PCR-Based microRNA Detection Method Based on Small Organic Dye and Single Enzyme., ANALYTICAL CHEMISTRY, 2018, 通讯作者
(33) Synthesis of asymmetrical thioethers with sulfinamides as the sulfenylation agent under metal-free conditions, TETRAHEDRON LETTERS, 2018, 通讯作者
(34) Using sulfinamides as high oxidation state sulfur reagent for preparation of sulfenamides, TETRAHEDRON LETTERS, 2018, 通讯作者
(35) DNAzyme based visual detection of DNA methylation, CHEMICAL COMMUNICATIONS, 2018, 通讯作者
(36) 重楼法定基原与其同属近缘种的多重PCR鉴别体系研究, Multiplex PCR System for the Identification of Paridis Rhizoma’s Legal Origins and Its Closely Related Species from Genus Paris, 天然产物研究与开发, 2018, 第 6 作者
(37) Evolution of microbial biosensor based on functional RNA through fluorescence-activated cell sorting, SENSORS AND ACTUATORS B-CHEMICAL, 2018, 通讯作者
(38) 华重楼及其常见混伪品的多重聚合酶链式反应快速鉴定体系的建立和应用, Development and Application of Multiplex PCR Method for Rapid Detection of Paris Polyphylla Smith var.Chinensis(Franch).Hara and It's Common Adulterants from Sichuan Province, 科学技术与工程, 2017, 第 4 作者
(39) BrOnsted-Acid-Catalyzed Substrate-Controlled and Site-Selective Friedel-Crafts Alkylation: A New Strategy for Post-Modification of 1,2-Dihydroquinolines, ASIAN JOURNAL OF ORGANIC CHEMISTRY, 2017, 通讯作者
(40) A Novel Colorimetric PCR-Based Biosensor for Detection and Quantification of Hepatitis B Virus., Methods in molecular biology (Clifton, N.J.), 2017, 第 5 作者
(41) Step-Controlled Povarov-Type Reaction with 1,2-Dihydroquinolines as Precursors of Dienophiles: Direct Synthesis of Spirocyclic Bi-tetrahydroquinolines and Functionalized 1,2-Dihydroquinolines., ORGANIC LETTERS, 2017, 通讯作者
(42) A tandem annulation with a 1,3-hydride transfer as the key step leading to isochromans., Chemical communications (Cambridge, England), 2017, 第 3 作者
(43) Bio-inspired enantioselective full transamination using readily available cyclodextrin, RSC ADVANCES, 2017, 通讯作者
(44) A one pot three-component reaction for the preparation of dihydroquinolines with two different ketones and aromatic amines, ORGANIC & BIOMOLECULAR CHEMISTRY, 2017, 第 4 作者
(45) Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR., SCIENTIFIC REPORTS, 2017, 通讯作者
(46) Selective tumor cell death induced by irradiated riboflavin through recognizing DNA G-T mismatch, NUCLEIC ACIDS RESEARCH, 2017, 通讯作者
(47) Kinetic resolution of indolines through reductive amination of aldehydes by chiral Brønsted acid, Tetrahedron Letters, 2017, 第 3 作者
(48) Re-characterization of hammerhead ribozymes as molecular tools for intermolecular RNA cleavage., ORGANIC & BIOMOLECULAR CHEMISTRY, 2017, 通讯作者
(49) The catalytic enantioselective synthesis of tetrahydroquinolines containing all-carbon quaternary stereocenters via the formation of aza-ortho-xylylene with 1,2-dihydroquinoline as a precursor, CHEMICAL COMMUNICATIONS, 2016, 通讯作者
(50) Sequence-Specific Biosensing of DNA Target through Relay PCR with Small-Molecule Fluorophore, ACS CHEMICAL BIOLOGY, 2016, 通讯作者
(51) Investigation and Application of Amphoteric alpha-Amino Aldehyde: An in Situ Generated Species Based on Heyns Rearrangement, ORGANIC LETTERS, 2016, 通讯作者
(52) 基于PCR的半夏水煎液中半夏药材定量检测方法研究, Quantitative Analysis of Pinellia ternata in Banxia Decoction by PCR, 中药材, 2016, 第 6 作者
(53) Characterization of deoxyribozymes with site-specific oxidative cleavage activity against DNA obtained by in vitro selection., ORGANIC & BIOMOLECULAR CHEMISTRY, 2016, 通讯作者
(54) 一种制备盐酸西那卡塞的新方法, A New Synthetic Method of Cinacalcet Hydrochloride, 合成化学, 2016, 第 3 作者
(55) GFP生色团衍生物的合成及其荧光性能, Synthesis and Fluorescence Properties of the GFP Chromophores' Derivatives, 合成化学, 2016, 第 5 作者
(56) 一种氨基酸衍生物消旋的新方法, A New Method for Racemization of Amino Acid Derivatives, 合成化学, 2016, 第 4 作者
(57) A general solution for opening double-stranded DNA for isothermal amplification., SCIENTIFIC REPORTS, 2016, 通讯作者
(58) Simple Bronsted acid catalyzed C-H functionalization: efficient access to poly-substituted pyridines, TETRAHEDRON LETTERS, 2016, 通讯作者
(59) Sequence-specific RNA Photocleavage by Single-stranded DNA in Presence of Riboflavin, SCIENTIFIC REPORTS, 2015, 通讯作者
(60) 全天麻胶囊中天麻的DNA鉴定, 中药与临床, 2015, 第 4 作者
(61) Selection of Intracellularly Functional RNA Mimics of Green Fluorescent Protein Using Fluorescence-Activated Cell Sorting, JOURNAL OF MOLECULAR EVOLUTION, 2015, 通讯作者
(62) Enantioselective Organocatalytic Transfer Hydrogenation of 1,2-Dihydroquinoline through Formation of Aza-o-xylylene, ORGANIC LETTERS, 2015, 第 6 作者
(63) The activation of IgM- or isotype-switched IgG- and IgE-BCR exhibits distinct mechanical force sensitivity and threshold, ELIFE, 2015, 第 10 作者
(64) Template-directed chemical ligation to obtain 3’-3’ and 5’-5’ phosphodiester DNA linkages., SCIENTIFIC REPORTS, 2014, 通讯作者
(65) In vitro selection of DNA-cleaving deoxyribozyme with site-specific thymidine excision activity, NUCLEIC ACIDS RESEARCH, 2014, 通讯作者
(66) RNA-primed allele-specific PCR, Science China Chemistry,, 2014, 通讯作者
(67) Signal amplification of glucosamine-6-phosphate based on ribozyme glmS, BIOSENSORS & BIOELECTRONICS, 2014, 第 7 作者
(68) Signal amplification of glucosamine-6-phosphate based on ribozyme glmS, Biosensors and Bioelectronics, 2014, 第 7 作者
(69) RNA-primed allele-specific PCR, SCIENCE CHINA-CHEMISTRY, 2014, 通讯作者
(70) 记忆B细胞与幼稚B细胞受单分子力学刺激活化差异的研究, 第九届全国免疫学学术大会, 2014, 第 7 作者
(71) A novel colorimetric PCR-based biosensor for detection and quantification of hepatitis B virus, ANALYTICA CHIMICA ACTA, 2014, 通讯作者
(72) Alkyl transfer from C-C cleavage: replacing the nitro group of nitro-olefins, CHEMICAL COMMUNICATIONS, 2014, 通讯作者
(73) 体外筛选剪切DNA的DNA酶, 应用与环境生物学报, 2013, 第 7 作者
(74) RNA pathogen detection with one-step reverse transcription PCR and strand-displacement based signal amplification, ANALYST, 2013, 通讯作者
(75) DNAzyme based gap-LCR detection of single-nucleotide polymorphism, BIOSENSORS & BIOELECTRONICS, 2013, 通讯作者
(76) Alkyl Transfer from C-C Cleavage, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2013, 通讯作者
(77) DNA display for drug discovery, RSC ADVANCES, 2013, 通讯作者
(78) 孔雀石绿和结晶紫在荧光检测中的比较, 应用与环境生物学报, 2013, 第 7 作者
(79) Cleavage-based signal amplification of RNA., NATURE COMMUNICATIONS, 2013, 通讯作者
(80) First way of enantioselective synthesis of moxifloxacin intermediate, SCIENCE CHINA-CHEMISTRY, 2013, 通讯作者
(81) Organocatalytic asymmetric Michael reaction with acylsilane donors., ORGANIC & BIOMOLECULAR CHEMISTRY, 2013, 第 5 作者
(82) First asymmetric intermolecular bromoesterification catalyzed by chiral Bronsted acid, TETRAHEDRON-ASYMMETRY, 2012, 第 5 作者
(83) EVOLUTIONAL AND FUNCTIONAL ANALYSIS OF A SERINE PROTEASE IN Spodoptera litura, ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY, 2012, 通讯作者
(84) 单取代苯乙醛及其二氢吡啶衍生物的合成, Synthesis of Single Replace Phenylacetaldehyde and Corresponding 1,4-Dihydropyridine Derivatives, 合成化学, 2012, 第 4 作者
(85) 生物素标记试剂的合成方法改进及其在DNA合成中的应用, Improvement Method on the Synthesis of a Biotin-labelled Reagent and Its Application in the DNA Synthesis, 合成化学, 2012, 第 2 作者
(86) Amplified detection of nucleic acid by G-quadruplex based hybridization chain reaction, BIOSENSORS & BIOELECTRONICS, 2012, 第 4 作者
(87) DNA based identification of medicinal materials in Chinese patent medicines, SCIENTIFIC REPORTS, 2012, 通讯作者
(88) Enantiodifferentiating photoisomerization of cyclooctene included and sensitized by benzoate modified β-cyclodextrin derivatives: switching of product chirality by solvent, Tetrahedron Letters, 2011, 第 4 作者
(89) Colorimetric Detection of PCR Product with DNAzymes Induced by 5 '-Nuclease Activity of DNA Polymerases, CHEMBIOCHEM, 2011, 通讯作者
(90) Site-selective DNA hydrolysis induced by a metal-free peptide nucleic acid-cyclen conjugate, CHEMICAL COMMUNICATIONS, 2011, 通讯作者
(91) Direct measurement of electrical transport through G-quadruplex DNA with mechanically controllable break junction electrodes., Angewandte Chemie (International ed. in English), 2010, 第 2 作者
(92) Novel DNA catalysts based on G-quadruplex recognition, CHEMBIOCHEM, 2008, 第 1 作者
(93) Proline-modified DNA as catalyst of the aldol reaction, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 第 1 作者
(94) Novel Organocatalyst Bearing Molecular Recognition Group, Organic letters, 2006, 第 1 作者
(95) Development of a Highly Efficient L-Prolinamide-Based Organocatalyst for Direct Aldol Reactions by Tuning the Electronic Property of the Substituent, J. Am. Chem. Soc, 2005, 第 1 作者
(96) Small peptides catalyze highly enantioselective direct aldol reactions of aldehydes with hydroxyacetone: unprecedented regiocontrol in aqueous media., ORGANIC LETTERS, 2004, 
(97) Proc Nat Aca Sci USA, 2004, 
(98) Novel small organic molecules for a highly enantioselective direct aldol reaction., JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 

合作情况

德国、巴基斯坦外籍青年科学家计划

项目协作单位

Universität Konstanz,Germany


University of the Punjab,Pakistan

招生信息

化学生物学;有机化学等相关专业。

在研项目

基于连续培养的体内核酶筛选体系国家任务-国家自然科学基金课题(面上项目)2016-01-012019-12-0177.0负责人 
万人计划其他任务-2015-01-012017-12-3130.0负责人 
优秀青年基金国家任务-2013-09-012016-09-01100.0负责人 
生物多样性保护与特色生物资源的可持续利用中国科学院计划-2013-04-012014-04-0110.0负责人 
横向课题其他任务-2013-02-012015-02-0110.0负责人 
中药标准化国家任务-2013-01-012015-12-3130.0参与 
转化医学平台地方任务-2013-01-012015-12-3120.0参与 
四川省****地方任务-2012-06-012015-06-3050.0负责人 
外籍青年科学家计划中国科学院计划-2011-10-012012-09-3026.0负责人 
面上基金国家任务-2011-09-292015-12-3160.0负责人