主要研究方向：

基因工程法对蛋白定点荧光标记与活细胞成像，用于微生物蛋白的活性和功能机制研究，包括病毒/细菌的感染机制、药物筛选，通过调节蛋白的组装调控功能蛋白活性等。

1. 细胞内蛋白质的标记：致力于开发新的蛋白标记策略。

2. 通过活细胞荧光成像研究蛋白的功能机制

3. 通过调节蛋白组装在体内/体外调控蛋白质活性

招收硕士研究生1名

0703Z1-化学生物学

2009-09--2014-06   吉林大学化学学院   博士研究生
2005-09--2009-06   吉林大学化学学院   学士

   

（ 1 ） 阻断COVID-19棘突状蛋白与人血管紧张素转化酶2结合的小分子抑制剂及其用途, 发明专利, 2021, 第 2 作者, 专利号: CN112457281A

（ 2 ） 一种鉴别临床多种多重耐药细菌的荧光检测法, 发明专利, 2020, 第 3 作者, 专利号: CN110724727A

（ 3 ） 一种对膜电荷响应的比率荧光探针及其在细菌检测的应用, 发明专利, 2019, 第 2 作者, 专利号: CN110511186A

（ 4 ） 用于标记SNAP-tag的蛋白标签荧光探针, 专利授权, 2021, 第 3 作者, 专利号: CN109400609B

（ 5 ） 一种用于细胞内蛋白标记的荧光探针及其合成方法和应用, 专利授权, 2020, 第 2 作者, 专利号: CN108069967B

（ 6 ） 用于SNAP蛋白标记的小分子荧光探针及其合成方法与应用, 专利授权, 2020, 第 4 作者, 专利号: CN108069966B

（ 7 ） 一种用于蛋白标记及检测的荧光探针及其合成方法与应用, 专利授权, 2019, 第 3 作者, 专利号: CN106867515B

（ 8 ） 一种检测血液中谷胱甘肽的荧光探针及其合成方法与应用, 专利授权, 2019, 第 2 作者, 专利号: CN106866689B

   

（1） FRET-based in vitro Assay for Rapid Detecting of SARS-CoV-2 Entry Inhibitors, Chin. Chem. Lett., 2025, 第 2 作者  通讯作者
（2） Enhancing the photostability of red fluorescent proteins through FRET with Si-rhodamine for dynamic super-resolution fluorescence imaging, Chemical Science, 2025, 第 2 作者  通讯作者
（3） Azetidinyl Malachite Green: a Superior Fluorogen-Activating Protein Probe for Live-cell and Dynamic SIM Imaging, Chemical Science, 2025, 第 5 作者
（4） Dye-mediated FRET Strategy for Constructing Semi-synthetic Large Stokes Shift Far-red Fluorescent Protein, Chin. Chem. Lett., 2025, 第 2 作者  通讯作者
（5） Naphthalimide-derived Fluorogenic SNAP Probe for Real-time Monitoring of Protein Degradation, Chem. Commun., 2025, 第 2 作者  通讯作者
（6） Super-resolution Imaging Reveals the Mechanism of Endosomal Acidification Inhibitors against SARS-CoV-2 Infection, ChemBioChem, 2024, 
（7） Live-Cell Imaging to Resolve Salt-Induced Liquid���Liquid Phase Separation of FUS Protein by Dye Self-Labeling, Chem. Biomed. Imaging, 2024, 
（8） Bacterial Membrane-induced Disassembly of Fluorescein Aggregates Enables Selective Imaging and Killing of Gram-positive Bacteria, Sensor. Actuat. B-Chem., 2024, 
（9） SIM imaging resolves endocytosis of SARS-CoV-2 spike RBD in living cells, CELL CHEMICAL BIOLOGY, 2023, 第 1 作者
（10） Dye disaggregation light-up aptamer for super-resolution RNA imaging, SENSORS AND ACTUATORS B-CHEMICAL, 2023, 第 2 作者  通讯作者
（11） Rapid screening of SARS-CoV-2 inhibitors via ratiometric fluorescence of RBD–ACE2 complexes in living cells by competitive binding, Rapid screening of SARS-CoV-2 inhibitors via ratiometric fluorescence of RBD���ACE2 complexes in living cells by competitive binding, ACTA PHARMACEUTICA SINICA B, 2022, 第 1 作者
（12） In Situ Real-Time Nanoscale Resolution of Structural Evolution and Dynamics of Fluorescent Self-Assemblies by Super-Resolution Imaging, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2022, 第 7 作者
（13） Diagnosis Biomarkers of Cholangiocarcinoma in Human Bile: An Evidence-Based Study, CANCERS, 2022, 第 4 作者
（14） Development of fluorescent probes targeting the cell wall of pathogenic bacteria, COORDINATION CHEMISTRY REVIEWS, 2021, 第 2 作者
（15） An assembly-regulated SNAP-tag fluorogenic probe for long-term super-resolution imaging of mitochondrial dynamics, BIOSENSORS & BIOELECTRONICS, 2021, 第 8 作者
（16） A Cell Membrane Fluorogenic Probe for Gram-Positive Bacteria Imaging and Real-Time Tracking of Bacterial Viability, ACS Appl. Bio Mater, 2021, 第 1 作者  通讯作者
（17） Multiple Factors Regulate the Spirocyclization Equilibrium of Si-Rhodamines, JOURNAL OF PHYSICAL CHEMISTRY B, 2020, 第 5 作者
（18） Fluorescent antibiotics for real-time tracking of pathogenic bacteria, Fluorescent antibiotics for real-time tracking of pathogenic bacteria, JOURNAL OF PHARMACEUTICAL ANALYSIS, 2020, 第 1 作者
（19） Native CRISPR-Cas-Mediated Genome Editing Enables Dissecting and Sensitizing Clinical Multidrug-Resistant P.aeruginosa, CELLREPORTS, 2019, 第 5 作者
（20） Self-assembling nanoprobes that display two-dimensional fluorescent signals for identification of surfactants and bacteria, CHEMICALCOMMUNICATIONS, 2019, 第 4 作者  通讯作者
（21） A general strategy to develop cell membrane fluorescent probes with location- and target-specific fluorogenicities: a case of a Zn2+ probe with cellular selectivity, CHEMICAL COMMUNICATIONS, 2019, 第 5 作者
（22） A h-bond strategy to develop acid-resistant photoswitchable rhodamine spirolactams for super-resolution single-molecule localization microscopy, CHEMICAL SCIENCE, 2019, 第 6 作者
（23） SNAP-tag蛋白标签技术与荧光分析法在蛋白原位分析中的联合应用, Combined application of SNAP-tag and fluorescence technique in in-situ protein analysis, 色谱, 2019, 第 5 作者
（24） A self-assembly/disassembly two-photo ratiometric fluorogenic probe for bacteria imaging, CHINESE CHEMICAL LETTERS, 2019, 第 3 作者
（25） Rapid identification of bacteria by membrane-responsive aggregation of a pyrene derivative, ACS SENSORS, 2019, 第 2 作者
（26） Strong π-π stacking interactions led to the mis-assignment of dimer emissions to the monomers of 1-acetylpyrene, Strong ��-�� stacking interactions led to the mis-assignment of dimer emissions to the monomers of 1-acetylpyrene, 中国化学快报:英文版, 2019, 第 3 作者
（27） Combined application of SNAP-tag and fluorescence technique in in-situ protein analysis, CHINESE JOURNAL OF CHROMATOGRAPHY, 2019, 第 5 作者
（28） Strong pi-pi stacking interactions led to the mis-assignment of dimer emissions to the monomers of 1-acetylpyrene, CHINESE CHEMICAL LETTERS, 2019, 第 3 作者
（29） A self-assembly/disassembly two-photo ratiometric fluorogenic probe for bacteria imaging, A self-assembly/disassembly two-photo ratiometric fluorogenic probe for bacteria imaging, 中国化学快报:英文版, 2019, 第 3 作者
（30） Strong ��-�� stacking interactions led to the mis-assignment of dimer emissions to the monomers of 1-acetylpyrene, CHINESECHEMICALLETTERS, 2019, 第 3 作者
（31） Aptamer based fluorescent probe for serum HER2-ECD detection: The clinical utility in breast cancer, Aptamer based fluorescent probe for serum HER2-ECD detection: The clinical utility in breast cancer, 中国化学快报：英文版, 2018, 第 4 作者
（32） Aptamer based fluorescent probe for serum HER2-ECD detection: The clinical utility in breast cancer, CHINESE CHEMICAL LETTERS, 2018, 第 4 作者  通讯作者
（33） SNAP-tag fluorogenic probes for wash free protein labeling, CHINESE CHEMICAL LETTERS, 2017, 第 4 作者  通讯作者
（34） A wash-free SNAP-tag fluorogenic probe based on the additive effects of quencher release and environmental sensitivity, CHEMICAL COMMUNICATIONS, 2017, 第 3 作者  通讯作者
（35） A naphthalimide-derived fluorogenic probe for SNAP-tag with a fast record labeling rate, DYES AND PIGMENTS, 2017, 第 6 作者  通讯作者
（36） SNAP-tag fluorogenic probes for wash free protein labeling, SNAP-tag fluorogenic probes for wash free protein labeling, 中国化学快报：英文版, 2017, 第 4 作者
（37） Cd2+-triggered amide tautomerization produces a highly Cd2+-selective fluorescent sensor across a wide pH range, DYES AND PIGMENTS, 2016, 第 5 作者
（38） Micelle-induced self-assembling protein nanowires: versatile supramolecular scaffolds for designing the light-harvesting system, ACS NANO, 2016, 第 3 作者
（39） The construction of functional protein nanotubes by small molecule-induced self-assembly of cricoid proteins, CHEMICAL COMMUNICATIONS, 2016, 第 1 作者
（40） A 1,8-naphthalimide-derived turn-on fluorescent probe for imaging lysosomal nitric oxide in living cells, CHINESE CHEMICAL LETTERS, 2016, 第 4 作者
（41） Self-Assembly of Cricoid Proteins Induced by "Soft Nanoparticles": An Approach To Design Multienzyme-Cooperative Antioxidative Systems, ACS NANO, 2015, 第 2 作者
（42） Quantum-Dot-Induced Self-Assembly of Cricoid Protein for Light Harvesting, ACS NANO, 2014, 第 1 作者
（43） Reversible Ca2+ Switch of An Engineered Allosteric Antioxidant Selenoenzyme, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2014, 第 5 作者
（44） Construction of a highly stable artificial glutathione peroxidase on a protein nanoring, ORGANIC & BIOMOLECULAR CHEMISTRY, 2014, 第 1 作者
（45） Highly Ordered Protein Nanorings Designed by Accurate Control of Glutathione S-Transferase Self-Assembly, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2013, 第 4 作者

   

（ 1 ） 基于环状荧光染料适配体的活细胞蛋白非共价标记和超分辨成像, 负责人, 国家任务, 2024-01--2027-12
（ 2 ） 胆管癌光动力治疗智能光敏药物研发, 负责人, 研究所自主部署, 2024-01--2025-12
（ 3 ） 临床细菌快速检测荧光探针法的研发, 负责人, 研究所自主部署, 2020-08--2022-07
（ 4 ） 以谷胱甘肽硫转移酶为受体构建专一识别谷胱甘肽的生物荧光探针及其生物应用, 负责人, 国家任务, 2016-01--2018-12

（1）微小蛋白的荧光标记新策略   中国化学会第34届学术年会   2024-06-15
（2）致病微生物的快速高灵敏诊断及其抑制剂筛选的研究   第五届荧光探针与成像青年学者研讨会   2021-05-16