Xuefeng Lu, 
Telephone: +86-532-80662712
Address:  No. 189, Songling Road, Laoshan District, Shandong Province, China
Postcode:  266101

Research Areas

Biofuels, biochemicals and biomedicines production by metabolic engineering of photosynthetic cyanobacteria and filamentous fungi

Education & & Employment

  • 2012-01 to present, Professor, Deputy Director-General of Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
  • 2008-05 to 2011-12, Professor, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
  • 2005-10 to 2008-04, Postdoctoral Fellow, Stanford University
  • 2000-08 to 2005-07, Ph. D., University of New Mexico
  • 1993-09 to 1997-07, B. S., Shanxi University



  1. Wang, M. and Lu, X.* (2016) Exploring the synergy between cellobiose dehydrogenase from Phanerochaete chrysosporium and cellulase from Trichoderma reesei. Frontiers in Microbiology, 7.

  2. Tan, X., Luo, Q. and Lu, X.* (2016) Biosynthesis, biotechnological production, and applications of glucosylglycerols. Appl. Microbiol. Biotechnol., 100, 6131-6139.

  3. Song, K., Tan, X.*, Liang, Y. and Lu, X.* (2016) The potential of Synechococcus elongatus UTEX 2973 for sugar feedstock production. Appl. Microbiol. Biotechnol., 100, 7865-7875.

  4. Huang, X., Chen, M., Li, J. and Lu, X.* (2016) Establishing an efficient gene-targeting system in an itaconic-acid producing Aspergillus terreus strain. Biotechnol. Lett., 38, 1603-1610.

  5. Chen, M., Huang, X., Zhong, C., Li, J. and Lu, X.* (2016) Identification of an itaconic acid degrading pathway in itaconic acid producing Aspergillus terreus. Appl. Microbiol. Biotechnol., 100, 7541-7548.

  6. Zhang, L., Liang, Y., Wu, W., Tan, X. and Lu, X.* (2016) Microbial synthesis of propane by engineering valine pathway and aldehyde-deformylating oxygenase. Biotechnol Biofuels, 9, 80.

  7. Zhu, T., Xie, X., Li, Z., Tan, X. and Lu, X.* (2015) Enhancing photosynthetic production of ethylene in genetically engineered Synechocystis sp. PCC 6803. Green Chem., 17, 421-434.

  8. Duan, Y., Luo, Q., Cai, K., Liang, F. and Lu, X.* (2015) Sucrose secreted by engineering cyanobacteria and its fermentability. J. Ocean Univ. China, 15, 890-896.

  9. Wu, W., Zhang, L., Yao, L., Tan, X., Liu, X. and Lu, X.* (2015) Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes. Sci Rep, 5, 10907.

  10. Tan, X., Du, W. and Lu, X.* (2015) Photosynthetic and extracellular production of glucosylglycerol by genetically engineered and gel-encapsulated cyanobacteria. Appl. Microbiol. Biotechnol., 99, 2147-2154.

  11. Savakis, P., Tan, X., Du, W., Branco dos Santos, F., Lu, X. and Hellingwerf, K.J. (2015) Photosynthetic production of glycerol by a recombinant cyanobacterium. J. Biotechnol., 195, 46-51.

  12. Luan, G., Qi, Y., Wang, M., Li, Z., Duan, Y., Tan, X. and Lu, X.* (2015) Combinatory strategy for characterizing and understanding the ethanol synthesis pathway in cyanobacteria cell factories. Biotechnol Biofuels, 8, 184.

  13. Jia, C., Li, M.*, Li, J., Zhang, J., Zhang, H., Cao, P., Pan, X., Lu, X.* and Chang, W.* (2015) Structural insights into the catalytic mechanism of aldehyde-deformylating oxygenases. Protein & Cell, 6, 55-67.

  14. Huang, X., Chen, M., Lu, X., Li, Y., Li, X. and Li, J.-J.* (2014) Direct production of itaconic acid from liquefied corn starch by genetically engineered Aspergillus terreus. Microbial Cell Factories, 13, 108.

  15. Yao, L., Qi, F., Tan, X. and Lu, X.* (2014) Improved production of fatty alcohols in cyanobacteria by metabolic engineering. Biotechnol Biofuels, 7, 94.

  16. Yang, H., Liao, L., Bo, T., Zhao, L., Sun, X., Lu, X., Norling, B. and Huang, F.* (2014) Slr0151 in Synechocystis sp. PCC 6803 is required for efficient repair of photosystem II under high-light condition. J Integr Plant Biol, 56, 1136-1150.

  17. Wang, Q., Huang, X., Zhang, J., Lu, X., Li, S. and Li, J.J*. (2014) Engineering self-sufficient aldehyde deformylating oxygenases fused to alternative electron transfer systems for efficient conversion of aldehydes into alkanes. Chem Commun (Camb), 50, 4299-4301.

  18. Liu, Y., Wang, C., Yan, J., Zhang, W., Guan, W., Lu, X. and Li, S.* (2014) Hydrogen peroxide-independent production of alpha-alkenes by OleTJE P450 fatty acid decarboxylase. Biotechnology for Biofuels, 7, 28.

  19. Huang, X., Lu, X., Li, Y., Li, X. and Li, J.J.* (2014) Improving itaconic acid production through genetic engineering of an industrial Aspergillus terreus strain. Microb Cell Fact, 13, 119.

  20. Huang, X., Lu, X. and Li, J.J.* (2014) Cloning, characterization and application of a glyceraldehyde-3-phosphate dehydrogenase promoter from Aspergillus terreus. J. Ind. Microbiol. Biotechnol., 41, 585-592.

  21. Bao, X., Huang, X., Lu, X. and Li, J.J.* (2014) Improvement of hydrogen peroxide stability of Pleurotus eryngii versatile ligninolytic peroxidase by rational protein engineering. Enzyme Microb. Technol., 54, 51-58.

  22. Zhang, J., Lu, X.* and Li, J.-J.* (2013) Conversion of fatty aldehydes into alk (a/e)nes by in vitro reconstituted cyanobacterial aldehyde-deformylating oxygenase with the cognate electron transfer system. Biotechnology for Biofuels, 6, 86.

  23. Du, W., Liang, F., Duan, Y., Tan, X. and Lu, X.* (2013) Exploring the photosynthetic production capacity of sucrose by cyanobacteria. Metab. Eng., 19, 17-25.

  24. Gao, Q., Tan, X. and Lu, X.* (2013) Enzymatic and physiological characterization of fatty acid activation in Synechocystis sp. PCC6803. J. Basic Microbiol., 53, 848-855.

  25. Liu, A., Tan, X., Yao, L. and Lu, X.* (2013) Fatty alcohol production in engineered E. coli expressing Marinobacter fatty acyl-CoA reductases. Appl. Microbiol. Biotechnol., 97, 7061-7071. Liu, A., Zhu, T., Lu, X.* and Song, L. (2013) Hydrocarbon profiles and phylogenetic analyses of diversified cyanobacterial species. Appl. Energ., 111, 383-393.

  26. Qi, F., Yao, L., Tan, X. and Lu, X.* (2013) Construction, characterization and application of molecular tools for metabolic engineering of Synechocystis sp. Biotechnol. Lett., 35, 1655-1661.

  27. Tan, X., Liang, F., Cai, K. and Lu, X.* (2013) Application of the FLP/FRT recombination system in cyanobacteria for construction of markerless mutants. Appl. Microbiol. Biotechnol., 97, 6373-6382.

  28. Wang, W., Liu, X. and Lu, X.* (2013) Engineering cyanobacteria to improve photosynthetic production of alka(e)nes. Biotechnology for Biofuels, 6, 69.

  29. Wang, W. and Lu, X.* (2013) Microbial Synthesis of Alka(e)nes. Front Bioeng Biotechnol, 1, 10.

  30. Yang, L., Zhu, Z., Wang, W. and Lu, X.* (2013) Microbial recycling of glycerol to biodiesel. Bioresour. Technol., 150, 1-8.

  31. Gao, Z.X., Zhao, H., Li, Z.M., Tan, X.M. and Lu, X.*F. (2012) Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria. Energy Environ Sci, 5.

  32. Tan, X., Wang, W. and Lu, X.* (2012) Production of photosynthetic biofuels by genetically engineering cyanobacteria. Current Chemical Biology, 6, 26-31.

  33. Gao, Q., Wang, W., Zhao, H. and Lu, X.* (2012) Effects of fatty acid activation on photosynthetic production of fatty acid-based biofuels in Synechocystis sp. PCC6803. Biotechnology for Biofuels, 5, 17.

  34. Bao, X., Liu, A., Lu, X. and Li, J.-J.* (2012) Direct over-expression, characterization and H2O2; stability study of active Pleurotus eryngiiversatile peroxidase in Escherichia coli. Biotechnol. Lett., 34, 1537-1543.

  35. Tan, X., Yao, L., Gao, Q., Wang, W., Qi, F. and Lu, X.* (2011) Photosynthesis driven conversion of carbon dioxide to fatty alcohols and hydrocarbons in cyanobacteria. Metab. Eng., 13, 169-176.

  36. Guan, W.*, Zhao, H., Lu, X.*, Wang, C., Yang, M. and Bai, F. (2011) Quantitative analysis of fatty-acid-based biofuels produced by wild-type and genetically engineered cyanobacteria by gas chromatography–mass spectrometry. J. Chromatogr., 1218, 8289-8293.

  37. Duan, Y., Zhu, Z., Cai, K., Tan, X. and Lu, X.* (2011) De novo Biosynthesis of Biodiesel by Escherichia coli in Optimized Fed-Batch Cultivation. PLoS ONE, 6, e20265.

  38. Lu, X.* (2010) A perspective: Photosynthetic production of fatty acid-based biofuels in genetically engineered cyanobacteria. Biotechnol. Adv., 28, 742-746.

  39. Wu, R., Reger, A.S., Lu, X., Gulick, A.M. and Dunaway-Mariano, D. (2009) The Mechanism of Domain Alternation in the Acyl-Adenylate Forming Ligase Superfamily Member 4-Chlorobenzoate: Coenzyme A Ligase. Biochemistry, 48, 4115-4125.

  40. Wu, R., Cao, J., Lu, X., Reger, A.S., Gulick, A.M. and Dunaway-Mariano, D. (2008) Mechanism of 4-chlorobenzoate: Coenzyme a ligase catalysis. Biochemistry, 47, 8026-8039.

  41. Lu, X., Vora, H. and Khosla, C. (2008) Overproduction of free fatty acids in E. coli: implications for biodiesel production. Metab. Eng., 10, 333-339.

  42. Li, L., Li, Z., Chen, D., Lu, X., Feng, X., Wright, E.C., Solberg, N.O., Dunaway-Mariano, D., Mariano, P.S., Galkin, A. et al. (2008) Inactivation of microbial arginine deiminases by L-canavanine. J. Am. Chem. Soc., 130, 1918-1931.

  43. Lu, X., Li, L., Wu, R., Feng, X., Li, Z., Yang, H., Wang, C., Guo, H., Galkin, A., Herzberg, O. et al. (2006) Kinetic analysis of Pseudomonas aeruginosa arginine deiminase mutants and alternate substrates provides insight into structural determinants of function. Biochemistry, 45, 1162-1172.

  44. Wu, J.B., Xu, D.G., Lu, X.F., Wang, C.H., Guo, H. and Dunaway-Mariano, D. (2006) Contributions of long-range electrostatic interactions to 4-chlorobenzoyl-CoA dehalogenase catalysis: A combined theoretical and experimental study. Biochemistry, 45, 102-112.

  45. Lu, X., Li, L., Feng, X., Wu, Y., Dunaway-Mariano, D., Engen, J.R. and Mariano, P.S. (2005) L-canavanine is a time-controlled mechanism-based inhibitor of Pseudomonas aeruginosa arginine deiminase. J. Am. Chem. Soc., 127, 16412-16413.

  46. Galkin, A., Lu, X., Dunaway-Mariano, D. and Herzberg, O. (2005) Crystal Structures Representing the Michaelis Complex and the Thiouronium Reaction Intermediate of Pseudomonas aeruginosa Arginine Deiminase. J. Biol. Chem., 280, 34080-34087.

  47. Lu, X., Galkin, A., Herzberg, O. and Dunaway-Mariano, D. (2004) Arginine deiminase uses an active-site cysteine in nucleophilic catalysis of L-arginine hydrolysis. J. Am. Chem. Soc., 126, 5374-5375.

  48. Gulick, A.M., Lu, X. and Dunaway-Mariano, D. (2004) Crystal structure of 4-chlorobenzoate : CoA ligase/synthetase in the unliganded and aryl substrate-bound states. Biochemistry, 43, 8670-8679.

  49. Dong, J., Lu, X., Wei, Y., Luo, L., Dunaway-Mariano, D. and Carey, P.R. (2003) The strength of dehalogenase-substrate hydrogen bonding correlates with the rate of Meisenheimer intermediate formation. Biochemistry, 42, 9482-9490.

  50. Dong, J., Carey, P.R., Wei, Y., Luo, L., Lu, X., Liu, R. and Dunaway-Mariano, D. (2002) Raman evidence for Meisenheimer complex formation in the hydrolysis reactions of 4-fluorobenzoyl- and 4-nitrobenzoyl-coenzyme a catalyzed by 4-chlorobenzoyl-coenzyme A dehalogenase. Biochemistry, 41, 7453-7463.



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