人工生命体设计；哺乳细胞基因线路设计与应用；有限代繁殖的病原菌疫苗；人工生命体的安全性与可控性；靶向巨噬细胞胞内结核杆菌的人工噬菌体设计

           招硕士生和博士生.

071010-生物化学与分子生物学
086000-生物与医药

合成生物学；疫苗开发
生物医药技术
定量生物学

2003-09--2009-06   北京大学物理学院与理论生物学中心   软凝聚态,博士
1999-09--2003-07   武汉大学,物理学院   物理学学士

   

2013-03~2019-06,中科院微生物所, 研究员
2011-09~2013-01,麻省理工学院(MIT), 博士后
2009-08~2011-08,加州大学旧金山分校(UCSF), 博士后

2018-07-30-2025-12-30,ACS Synthetic Biology, 编委
2017-12-31-2026-12-30,Synthetic and Systems Biotechnology, 编委

微生物代谢工程

   

（ 1 ） 细胞间通讯系统的全新设计方法、细胞 间通讯系统及其用途, 发明专利, 2020, 第 1 作者, 专利号: 202010461862.X

（ 2 ） 用于处理重金属污染的微生物细胞和蛋白、相应的处理方法和试剂盒, 发明专利, 2017, 第 1 作者, 专利号: CN107916243A

（ 3 ） 阻遏蛋白、调控元件组和基因表达调控系统及其构建方法, 发明专利, 2017, 第 1 作者, 专利号: CN107344962A

（ 4 ） 阻遏蛋白、调控元件组和基因表达调控系统及其构建方法, 发明专利, 2017, 第 1 作者, 专利号: CN107344962A

（ 5 ） Novel oligo-linker-mediated DNA assembly method and applications thereof, 发明专利, 2016, 第 1 作者, 专利号: U.S. Patent Application No. 2016/0340670 A1

（ 6 ） Methods and Kits For Fragmenting DNA, 发明专利, 2016, 第 3 作者, 专利号: U.S. Patent Application No. 62/156,417. /PCT/IL2016/050394

（ 7 ） 核酸的检测方法, 发明专利, 2015, 第 1 作者, 专利号: CN105177110A

（ 8 ） 基于CRISPR/Cas9和酿酒酵母细胞内源的同源重组的分子克隆方法, 发明专利, 2015, 第 1 作者, 专利号: CN105624146A

（ 9 ） Synthetic Biology Tools, 发明专利, 2013, 第 1 作者, 专利号: US Patent Application No: 2013/0005,590

   

[1] Chenrui Qin, Yanhui Xiang, Jie Liu, Ruilin Zhang, Ziming Liu, Tingting Li, Zhi Sun, Xiaoyi Ouyang, Yeqing Zong, Haoqian M. Zhang, Qi Ouyang, Long Qian, Chunbo Lou. Precise programming of multigene expression stoichiometry in mammalian cells by a modular and programmable transcriptional system. NATURE COMMUNICATIONS[J]. 2023, 14(1): 1-10, http://dx.doi.org/10.1038/s41467-023-37244-y.
[2] Chen, Xinmao, Wang, Tianze, Guan, Ying, Ouyang, Qi, Lou, Chunbo, Qian, Long. The Topological Characteristics of Biological Ratio-Sensing Networks. LIFE-BASEL[J]. 2023, 13(2): http://dx.doi.org/10.3390/life13020351.
[3] Yusuke Kato, Chunbo Lou. Synthetic Genetic Elements, Devices, and Systems. LIFE[J]. 2022, 12(945): https://doaj.org/article/6d65583491b64dc6a295135ea1f6c7a7.
[4] Zhao, Xuejin, Wei, Weijia, Zong, Yeqing, Bai, Chaoxian, Guo, Xian, Zhu, Hua, Lou, Chunbo. Novel switchable ECF sigma factor transcription system for improving thaxtomin A production in Streptomyces. SYNTHETIC AND SYSTEMS BIOTECHNOLOGY[J]. 2022, 7(3): 972-981, http://lib.cqvip.com/Qikan/Article/Detail?id=7108197529.
[5] Sun, Zhi, Wei, Weijia, Zhang, Mingyue, Shi, Wenjia, Zong, Yeqing, Chen, Yihua, Yang, Xiaojing, Yu, Bo, Tang, Chao, Lou, Chunbo. Synthetic robust perfect adaptation achieved by negative feedback coupling with linear weak positive feedback. NUCLEIC ACIDS RESEARCH[J]. 2022, 50(4): 2377-2386, http://dx.doi.org/10.1093/nar/gkac066.
[6] Xinmao Chen, Ziming Liu, Chunbo Lou, Ying Guan, Qi Ouyang, Yanhui Xiang. Improving cooperativity of transcription activators by oligomerization domains in mammalian cells. SYNTHETIC AND SYSTEMS BIOTECHNOLOGY. 2022, 8(1): 114-120, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9804245/.
[7] Xuejin Zhao, Yeqing Zong, Weijia Wei, Chunbo Lou, Pasquale Stano, Jun Ni. Multiplexed Promoter Engineering for Improving Thaxtomin A Production in Heterologous Streptomyces Hosts. LIFE. 2022, 12(5): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146380/.
[8] Guan, Ying, Chen, Xinmao, Shao, Bin, Ji, Xiangyu, Xiang, Yanhui, Jiang, Guoqiang, Xu, Lina, Lin, Zhanglin, Ouyang, Qi, Lou, Chunbo. Mitigating Host Burden of Genetic Circuits by Engineering Autonegatively Regulated Parts and Improving Functional Prediction. ACS SYNTHETIC BIOLOGY[J]. 2022, 11(7): 2361-2371, [9] Chen, Zehua, Sun, Jinyuan, Guan, Ying, Li, Ming, Lou, Chunbo, Wu, Bian. Engineered DNase-inactive Cpf1 variants to improve targeting scope for base editing in E. coli. SYNTHETIC AND SYSTEMS BIOTECHNOLOGY[J]. 2021, 6(4): 326-334, http://dx.doi.org/10.1016/j.synbio.2021.09.002.
[10] Pei Du, Chunbo Lou, Xuejin Zhao, Qihui Wang, Xiangyu Ji, Weijia Wei, Llus Ribas de Pouplana. CRISPR-Based Genetic Switches and Other Complex Circuits: Research and Application. LIFE[J]. 2021, 11(11): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621321/.
[11] Pei Du, Huiwei Zhao, Haoqian Zhang, Ruisha Wang, Jianyi Huang, Ye Tian, Xudong Luo, Xunxun Luo, Min Wang, Yanhui Xiang, Long Qian, Yihua Chen, Yong Tao, Chunbo Lou. De novo design of an intercellular signaling toolbox for multi-channel cell-cell communication and biological computation. NATURE COMMUNICATIONS[J]. 2020, 11(1): http://dx.doi.org/10.1038/s41467-020-17993-w.
[12] Ji, Xiangyu, Zhao, Huiwei, Zhu, Hua, Zhu, Kun, Tang, ShuangYan, Lou, Chunbo. CRISPRi/dCpf1-mediated dynamic metabolic switch to enhance butenoic acid production in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2020, 104(12): 5385-5393, https://www.webofscience.com/wos/woscc/full-record/WOS:000528992800001.
[13] Zheng, Yang, Meng, Fankang, Zhu, Zihui, Wei, Weijia, Sun, Zhi, Chen, Jinchun, Yu, Bo, Lou, Chunbo, Chen, GuoQiang. A tight cold-inducible switch built by coupling thermosensitive transcriptional and proteolytic regulatory parts. NUCLEIC ACIDS RESEARCH[J]. 2019, 47(21): e137-e137, http://dx.doi.org/10.1093/nar/gkz785.
[14] Miao, Chensi, Zhao, Huiwei, Qian, Long, Lou, Chunbo. Systematically investigating the key features of the DNase deactivated Cpf1 for tunable transcription regulation in prokaryotic cells. SYNTHETIC AND SYSTEMS BIOTECHNOLOGY[J]. 2019, 4(1): 1-9, http://lib.cqvip.com/Qikan/Article/Detail?id=7103844987.
[15] Hou, Junran, Zeng, Weiqian, Zong, Yeqing, Chen, Zehua, Miao, Chensi, Wang, Baojun, Lou, Chunbo. Engineering the Ultrasensitive Transcription Factors by Fusing a Modular Oligomerization Domain. ACS SYNTHETIC BIOLOGY[J]. 2018, 7(5): 1188-1194, http://dx.doi.org/10.1021/acssynbio.7b00414.
[16] 孟凡康, 娄春波. 人工遗传改造生命体的防逃逸技术研究进展. 有机化学[J]. 2018, 38(9): 2231-2242, http://lib.cqvip.com/Qikan/Article/Detail?id=676409203.
[17] Zhang, Yihao, Qian, Long, Wei, Weijia, Wang, Yu, Wang, Beining, Lin, Pingping, Liu, Wenchao, Xu, Luze, Li, Xiang, Liu, Dongming, Cheng, Sida, Li, Jiaofeng, Ye, Yixuan, Li, Hang, Zhang, Xiaohan, Dong, Yiming, Zhao, Xuejin, Liu, Cuihua, Zhang, Haoqian M, Ouyang, Qi, Lou, Chunbo. Paired Design of dCas9 as a Systematic Platform for the Detection of Featured Nucleic Acid Sequences in Pathogenic Strains. ACS SYNTHETIC BIOLOGY[J]. 2017, 6(2): 211-216, https://www.webofscience.com/wos/woscc/full-record/WOS:000394736400006.
[18] Tang, Qiang, Lou, Chunbo, Liu, ShuangJiang. Construction of an easy-to-use CRISPR-Cas9 system by patching a newly designed EXIT circuit. JOURNAL OF BIOLOGICAL ENGINEERING[J]. 2017, 11(1): http://dx.doi.org/10.1186/s13036-017-0072-5.
[19] 季翔宇, 赵会伟, 娄春波. 人工基因元件的生理学研究进展. 生物工程学报[J]. 2017, 33(3): 393-403, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=5948624&detailType=1.
[20] Yeqing Zong, Haoqian M Zhang, Cheng Lyu, Xiangyu Ji, Junran Hou, Xian Guo, Qi Ouyang, Chunbo Lou. Insulated transcriptional elements enable precise design of genetic circuits. NATURE COMMUNICATIONS[J]. 2017, 8(1): https://doaj.org/article/3e5ebc6575034de280e65ca0e1e373fd.
[21] Li, Teng, Ye, Jianwen, Shen, Rui, Zong, Yeqing, Zhao, Xuejin, Lou, Chunbo, Chen, GuoQiang. Semirational Approach for Ultrahigh Poly(3-hydroxybutyrate) Accumulation in Escherichia coli by Combining One-Step Library Construction and High-Throughput Screening. ACS SYNTHETIC BIOLOGY[J]. 2016, 5(11): 1308-1317, https://www.webofscience.com/wos/woscc/full-record/WOS:000388428100015.
[22] Fang, Mingyue, Wang, Tianmin, Zhang, Chong, Bai, Jili, Zheng, Xiang, Zhao, Xuejin, Lou, Chunbo, Xing, XinHui. Intermediate-sensor assisted push-pull strategy and its application in heterologous deoxyviolacein production in Escherichia coli. METABOLIC ENGINEERING[J]. 2016, 33: 41-51, http://dx.doi.org/10.1016/j.ymben.2015.10.006.
[23] Bai, Chaoxian, Zhang, Yang, Zhao, Xuejin, Hu, Yiling, Xiang, Sihai, Miao, Jin, Lou, Chunbo, Zhang, Lixin. Exploiting a precise design of universal synthetic modular regulatory elements to unlock the microbial natural products in Streptomyces. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2015, 112(39): 12181-12186, http://dx.doi.org/10.1073/pnas.1511027112.
[24] Jiang, Wenjun, Zhao, Xuejin, Gabrieli, Tslil, Lou, Chunbo, Ebenstein, Yuval, Zhu, Ting F. Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters. NATURE COMMUNICATIONS[J]. 2015, 6: http://dx.doi.org/10.1038/ncomms9101.
[25] Lou, Chunbo, Stanton, Brynne, Chen, YingJa, Munsky, Brian, Voigt, Christopher A. Ribozyme-based insulator parts buffer synthetic circuits from genetic context. NATURE BIOTECHNOLOGY[J]. 2012, 30(11): 1137-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000311087500034.
[26] Moon, Tae Seok, Lou, Chunbo, Tamsir, Alvin, Stanton, Brynne C, Voigt, Christopher A. Genetic programs constructed from layered logic gates in single cells. NATURE[J]. 2012, 491(7423): 249-253, http://dx.doi.org/10.1038/nature11516.
[27] Lou, Chunbo, Liu, Xili, Ni, Ming, Huang, Yiqi, Huang, Qiushi, Huang, Longwen, Jiang, Lingli, Lu, Dan, Wang, Mingcong, Liu, Chang, Chen, Daizhuo, Chen, Chongyi, Chen, Xiaoyue, Yang, Le, Ma, Haisu, Chen, Jianguo, Ouyang, Qi. Synthesizing a novel genetic sequential logic circuit: a push-on push-off switch. MOLECULAR SYSTEMS BIOLOGY[J]. 2010, 6: 350-350, https://www.webofscience.com/wos/woscc/full-record/WOS:000276310300002.
[28] Lou, Chunbo, Li, Zhengyan, Qi Ouyang. A molecular model for persister in E. coli. JOURNAL OF THEORETICAL BIOLOGY[J]. 2008, 255(2): 205-209, http://dx.doi.org/10.1016/j.jtbi.2008.07.035.
[29] Lou, Chunbo, Yang, Xiaojing, Liu, Xili, He, Bin, Qi Ouyang. A quantitative study of lambda-phage SWITCH and its components. BIOPHYSICALJOURNAL[J]. 2007, 92(8): 2685-2693, https://www.webofscience.com/wos/woscc/full-record/WOS:000245164000008.
[30] Yihao Zhang, Yu Wang, Luze Xu, Chunbo Lou, Qi Ouyang, Long Qian. Paired dCas9 design as a nucleic acid detection platform for pathogenic strains. METHODS. http://dx.doi.org/10.1016/j.ymeth.2021.06.003.