Our research focuses on the regulation of carbon assimilation in algae and crop. In particular, the regulation of carbon fixation enzyme Rubisco is hindered by the incomprehension of biogenesis pathways. Prokaryotic hexadecameric Rubisco is reconstituted in vitro by the sequential assistance of folding chaperonin and assembly chaperone, pointing to the complex biogenesis pathway in both prokaryotic and eukaryotic organisms. Research goals include identification of factors involved in the biogenesis of eukaryotic Rubisco, reconstitution of Rubisco with chaperones and regulation of Rubisco in algae and plants. Another study aims to analyse the enzymes involved in the Calvin cycles from common wheat. The research goal is to create high active enzyme after comparing the enzyme activities among copies, varieties and species and check the growth effect after transfer the created genes into plants.

   

Ph. D. University of Freiburg, Germany; M. S. Nankai University; B. S. nner Mongolia University

   

2011-Now:Group leader in Institute of Genetics and Developmental Biology, Chinese Academy of Sciences; 2006-2010:Postdoctor in the group of Prof. F. U. Hartl, Department of Cellular Biochemistry, Max-Planck-Institute of Biochemistry

   

(1) 4-磷酸赤藓糖的检测方法；patent number：ZL202111331959.X；applicants：刘翠敏;安向向;张文娟

(2) 6-磷酸葡萄糖异构酶在调控植物淀粉含量和生物量中的应用；patent number：ZL202011039773.2；PCT/CN2021/118919 ；applicants：刘翠敏;高飞;张慧君;张文娟

(3)大肠杆菌分子伴侣GroEL/ES在协助合成植物Rubisco中的应用；patent number：ZL201911084916.9 PCT_CN2020_126891；applicants：刘翠敏;刘小强;胡丽霞

(4)TaGPI1mS543A蛋白及其编码基因和应用；patent number：ZL201611033436.6；applicants：高飞;刘翠敏

(5)   TuVIPP1蛋白及其编码基因与应用；patent number：ZL201510468572.7；applicants：ZL201510468572.7

Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase), an enzyme catalyzed the rate limiting step of carbon assimilation, is infamous for its sluggish kinetics which have resulted in it being the world’s most abundant protein. The significant plasticity in the catalytic properties of natural Rubiscos suggests the changes can be introduced to generate better Rubisco. But the genetic manipulation of Rubisco has been hampered by the unclear of its biogenesis pathway. Our aim is to understand the regulation of the carbon fixation enzyme Rubisco, an undertaking that requires further understanding of its biogenesis. Active prokaryotic hexadecameric Rubisco is reconstituted in vitro via the sequential action of chaperonin and chaperone machinery for folding and assembly, pointing toward a complex biogenesis pathway in both prokaryotic and eukaryotic organisms. First, newly synthesized Rubisco large subunit (RbcL) is captured by chaperonin system to allow the RbcL fold to correct conformation. Second, the assembly chaperone RbcX interacts predominantly with C-terminal of RbcL and stabilize dimeric RbcL. Finally, nuclear translated rubisco small subunit (RbcS) replaced RbcX to form the haloenzyme. But active enkaryotic Rubisco could not generated by same procedure, pointing to the more complicated biogenesis pathway. Our research goals include identification of factors involved in the biogenesis of eukaryotic Rubisco and its regulation in algae and plants, and reconstitution of eukaryotic Rubisco with chaperones in vitro. Now we analyzed that chaperonin system in chloroplast is largely different from enkaryotic ones and we are trying to identify other cofactors in eukaryotic cells.

Our other specific aim is to analyse the enzymes involved in the Calvin cycle from common wheat. Here the main goal is to engineer highly active enzymes and then assessing the effect on growth after transfer of engineered genes into plants. The improved enzyme will be engineered by gene shuffling after comparing the enzyme activities among copies, varieties and species or by site-directed mutagenesis after solving their crystal structures.

   

(1) 10th Congress and 20th Symposium of algal branch, 2019, Regulation of chloroplast protein homeostasis.

(2) 9th Asia and Oceania Conference on Photobiology, 2019, Structure and Function of Chloroplast Chaperonin System of Chlamydomonas.

(3) Bilateral Symposium on wheat genome and molecular breeding between China and Germany, 2019, Structure and function of key protein of wheat starch content.

(4) The first academic symposium on Chlamydomonas molecular cells in China, 2019,  Chloroplast protein homeostasis regulated by chaperones and proteases.

(5) National Symposium on Photosynthesis, 2019, Biogenesis and Regulation of Rubisco.

(6) Cryo-EM structure of the chloroplast chaperonins, 2018, 1st Asia-Oceania International Congress on Photosynthesis.

(7) Youth Forum on "frontier and cross field of life science", 2018, Biosynthesis of Rubisco.

(8) 18th International Conference on the Cell and Molecular Biology of Chlamydomonas, 2018, Structure and Function of Chloroplast Chaperonin System of Chlamydomonas.

(9) National Congress of Plant Biology, 2016, The Crystal Structure of Chloroplast CPN60 from Chlamydomonas reinhardtii and Functional Partition of Different Subunits.

(10) 2nd Female Scientists Symposiumof Plant Biology, 2015, The structure and function of chloroplast Cpn60.

(11) 3rd International Conference on Plant Metabolism, 2014, The composition and structure of chloroplast chaperonin.

(12) National Congress of Plant Biology, 2013, Three chloroplast chaperonin protomers with various roles constitute authentic oligomers in Chlamydomonas reinhardtii.

已指导学生

国鹏  博士研究生  071009-细胞生物学  

柏翠翠  博士研究生  071009-细胞生物学  

张世佳  博士研究生  071009-细胞生物学  

马远征  博士研究生  071007-遗传学  

贾立加  博士研究生  071007-遗传学  

江姗  博士研究生  071007-遗传学  

胡丽霞  博士研究生  071011-生物物理学  

赵骞  博士研究生  071007-遗传学  

宋辉  博士研究生  071011-生物物理学  

张慧君  博士研究生  071011-生物物理学  

王宁  博士研究生  071011-生物物理学  

邵瑞琪  博士研究生  071011-生物物理学  

安向向  博士研究生  071011-生物物理学  

塔娜  博士研究生  071007-遗传学  

王冉  博士研究生  071011-生物物理学  

黄金兰  博士研究生  071011-生物物理学  

刘亚男  博士研究生  071011-生物物理学  

张馨  博士研究生  071011-生物物理学  

肖爽  硕士研究生  071007-遗传学  

现指导学生

单张凡  博士研究生  071007-遗传学  

梅乐  博士研究生  071007-遗传学  

何雨洁  博士研究生  071011-生物物理学  

王祎宁  博士研究生  071011-生物物理学  

李少飞  硕士研究生  071007-遗传学  

白向东  博士研究生  071007-遗传学  

张琛  博士研究生  071011-生物物理学  

刘会丽  博士研究生  071007-遗传学  

朱佳丽  硕士研究生  071007-遗传学  

茹一  博士研究生  071011-生物物理学  

袁圣雲  博士研究生  071007-遗传学  

黄博远  博士研究生  071008-发育生物学  

王尧  硕士研究生  071007-遗传学  

   

The Fifth Rebeiz Foundation for Basic Research (RFFBR) Paper Award for 2010