Environmental chemical biology and health

(a) speciation analysis of arsenic in environmental and biological systems,

(b) ultra-sensitive assays for the detection and imaging of biomolecules, 

(c) construction of target-triggered DNA nanomachine, 

(d) development of CRISPR/Cas9 based nano-technologies for editing specific gene,

(e) development of CRISPR-mediated molecular assays, including diagnostics of COVID-19.

Dr. Hanyong Peng is a professor in Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, China. He received B.Sc. in Chemistry (2008) and Ph.D. in Analytical Chemistry (2014), both from Wuhan University (Wuhan, China). With the support from China Scholarship Council, he pursued his doctoral research (2011-2013) and post-doctoral fellow (2015-2020)  in the Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Canada. 

1. Aijiao Yuan; Rui Sha; Wenjing Xie; Guangbo Qu; Hongquan Zhang; Hailin Wang; X. Chris Le; Guibin Jiang; Hanyong Peng*; RNA-Activated CRISPR/Cas12a Nanorobots Operating in Living Cells, Journal of the American Chemical Society, 2024, 146(39)：26657-26666.

  2. Wenjing Xie+; Qiangjun Hao+; Zi Ye+; Rui Sha; Bei Wen; Hailin Wang; Hongquan Zhang; Guohua Jia; X. Chris Le; Guibin Jiang; and Hanyong Peng*; Spherical Nucleic Acids-Directed Cryosynthesis of Manganese Nanoagents for Tumor Imaging and Therapy, Angewandte Chemie International Edition, 2025, e202503004.

  3. Zi Ye; Wenjing Liao; Zhaojia Deng; Lingfeng Wang; Bei Wen; Dapeng Zhang; Hailin Wang; Wenjing Xie; Hanyong Peng*; Fast-track synthesis of DNA-functionalized gold nanoparticles for biosensing applications, TrAC Trends in Analytical Chemistry, 2024, 175：117724.

  4. Benyu He; Lingfeng Wang; Xinyu Jin; Xing Zhang; Rui Sha; Yong Liang; Yawei Wang; Wenjing Xie; Jianbo Shi; Hanyong Peng*; Porous Agarose Layered Magnetic Graphene Oxide Nanocomposite for Virus RNA Monitoring in Wastewater, Analytical Chemistry, 2024, 96(22)：9167-9176.

  5. Aijiao Yuan; Tianrui Sun; Leyuan Chen; Dapeng Zhang; Wenjing Xie; Hanyong Peng*; CRISPR/Cas12a Corona Nanomachine for Detecting Circulating Tumor Nucleic Acids in Serum, Analytical Chemistry, 2024, 96(50)：20074-20081.

  6. Aijiao Yuan; Huifang Hao; Rui Sha; Hang Xiao; Fan Yang; Bo Pang; Jinhua Li*; Minghua Jin; Wenjing Xie; Lixia Zhao; Yawei Wang; Yu Zhang; Juan Li; Hanyong Peng*; In Situ Imaging of Cellular Inflammatory Response to Antibiotic Exposure with a DNAzyme Nanorobot, Environmental Science Technology, 2024, 58(46)：20619-20629.

  7. Leyuan Chen; Aijiao Yuan; Dapeng Zhang; Wenjing Xie; Hanyong Peng*; Fluorescence and colorimetric analysis of β-estradiol based on aptamer assembled spherical nucleic acids, Analytical Methods, 2024, 16：6356-6363.

  8. Zhaojia Deng; Wenjing Liao; Liangyun Bu; Jinhua Li; Juan Li; Lingfeng Wang; Aijiao Yuan; Wenjing Xie; Yawei Wang; Hanyong Peng*; Advancements in CRISPR-Diagnostic Techniques for Rapid on-site Monitoring of Environmental Virus, TrAC Trends in Analytical Chemistry, 2024, 181：118046.

  9. Xiaoyan Wang; Wenjing Xie; Hanyong Peng*; Screening Environmental Pollutants Binding Aptamers through Capture-SELEX for Biosensing Assays: Challenges and Opportunities, Environment & Health, 2023, 1(2)：72-74.

  10. Aijiao Yuan; Hang Xiao; Fan Yang; Huifang Hao; Xiaoyan Wang; Jinhua Li; Minghua Jin; Qiang Zhao; Rui Sha; Zhaojia Deng; Hanyong Peng*; DNA walker for signal amplification in living cells, TrAC Trends in Analytical Chemistry, 2022, 158：116870.

  11. Feng, Wei; Peng, Hanyong; Xu, Jingyang; Liu, Yanming; Pabbaraju, Kanti; Tipples, Graham; Joyce, Michael A.; Saffran, Holly A.; Tyrrell, D. Lorne; Babiuk, Shawn; Zhang, Hongquan; Le, X. Chris; Integrating Reverse Transcription Recombinase Polymerase Amplification with CRISPR Technology for the One-Tube Assay of RNA. Analytical Chemistry, 2021, 93(37): 12808-12816.

  12. Peng, H. Y., Le, C., Wu, J. J., Li, X. -F., Zhang, H. Q., Le, X. C. A genome editing nanomachine constructed with a clustered regularly interspaced short palindromic repeats system and activated by near-Infrared illumination. ACS Nano 2020, 14, 2817–2826.

  13. Wu, J. J.# , Peng, H. Y.#. , Lu, X. F., Lai, M., Zhang, H. Q., Le, X. C. Binding-mediated formation of ribonucleoprotein corona for efficient delivery and control of CRISPR-Cas9. Angewandte Chemie International Edition 2020, doi.org/10.1002/anie.202014162. (Co-first authors)

  14. Pang, B., Xu, J. Y., Liu, Y. M., Peng, H. Y., Feng, W., Cao, Y. R., Wu, J. J., Xiao, H. Y., Pabbaraju, K., Tipples, G., Joyce, M. A., Saffran, H. A., Tyrrell, D. L., Zhang, H. Q. and Le, X. C. Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR Technology, Analytical Chemistry 2020, 92, 16204–16212.

  15. Feng, W.#, Newbigging, A. M.#, Le, C.#, Pang, B.#, Peng, H. Y.#, Cao, Y. Wu, J. J., Abbas, G., Song, J., Wang, D. -B. Cui, M., Tao, J., Tyrrell, D. L., Zhang, X. -E., Zhang, H. Q., Le, X. C. Molecular diagnosis of COVID-19: Challenges and research needs. Analytical Chemistry 2020, 92, 10196–10209. (Co-first authors, Cover feature)

  16. Peng, H. Y., Newbigging, A., Reid, M. S., Uppal, J. S., Xu, J., Zhang, H., Le, X. C. Signal amplification in living cells: A review of microRNA detection and imaging. Analytical Chemistry 2020, 92, 292-308.

  17. Huang, K., Peng, H. Y., Gao, F., Liu, Q. Q., Lu, X. -F., Shen, Q. R., Le, X. C., Zhao, F. J. Biotransformation of arsenic-containing Roxarsone by an aerobic soil bacterium Enterobacter sp. CZ-1. Environmental Pollution 2019, 247, 482-487.

  18. Peng, H. Y., Newbigging, A., Wang, Z., Tao, J., Deng, W., Le, X. C., Zhang, H. Q. DNAzyme-mediated assays for amplified detection of nucleic acids and proteins. Analytical Chemistry 2018, 90, 190-207.

  19. Peng, H. Y., Hu, B., Liu, Q. Q., Li, X. -F., Zhang, H. Q., Le, X. C. Methylated phenylarsenical metabolites discovered in chicken liver. Angewandte Chemie International Edition 2017, 56, 6773–6777. (Cover feature)

  20. Peng, H. Y., Zhang, H., Li, X. -F., Le, X. C. A microRNA-initiated DNAzyme motor operating in living cells. Nature Communications 2017, 8, 14378. (Highly cited)

  21. Yang, Z. L., Peng, H. Y., Lu, X. F., Liu, Q. Q. Huang, R. F., Hu, B., Kachanoski, G., Zuidhof, M. J., Le, X. C. Arsenic metabolites, including N-acetyl-4-hydroxy-m-arsanilic acid, in chicken litter from a roxarsone-feeding study involving 1600 chickens. Environmental Science & Technology 2016, 50, 6737-6743.

  22. Liu, Q. Q., Peng, H. Y., Lu, X. F., Zuidhof, M. J., Li, X. -F., Le, X. C. Arsenic species in chicken breast: temporal variations of metabolites, elimination kinetics, and residual Concentrations. Environmental Health Perspectives 2016, 124, 1174-1181.

  23. Yan, X. W., Li, J. H., Liu, Q. Q., Peng, H. Y. Popowich, A., Wang, Z. X., Li, X. -F., Le, X. C. p-Azidophenylarsenoxide: A novel arsenical “bait” for the in-situ capture and identification of cellular arsenic-binding proteins. Angewandte Chemie-International Edition 2016, 55, 14051-14057.

  24. Zhang, H., Zuehlke, A., Peng, H. Y., Lai, M., Li, X. -F., Le, X. C. Binding-induced DNA nanomachines triggered by proteins and nucleic acids. Angewandte Chemie International Edition 2015, 54, 14326-14330.

  25.Peng, H. Y., Reid, M. S., Le, X. C. Consumption of rice and fish in an electronic waste recycling area contributes significantly to total daily intake of mercury. Journal of Environmental Sciences 2015, 38, 83-86.

 26. Peng, H. Y., Zhang, N., He, M., Chen, B. B., Hu, B. Simultaneous speciation analysis of inorganic arsenic, chromium and selenium in environmental waters by 3-(2-aminoethylamino) propyltrimethoxysilane modified multi-wall carbon nanotubes packed microcolumn solid phase extraction and ICP-MS. Talanta 2015, 131, 266–272. (Highly cited)

 27. Peng, H. Y., Hu, B., Liu, Q. Q., Yang, Z. L., Lu, X., Huang, R. F., Li, X. -F., Le X. C. Liquid chromatography combined with atomic and molecular mass spectrometry for speciation of arsenic in chicken liver. Journal of Chromatography A 2014, 1370, 40–49.

 28. Peng, H. Y., Jiao, Y., Xiao, X., Chen, B. B., He, M., Liu, Z. R., Zhang, X., Hu, B. Magnetic quantitative analysis for multiplex glycoprotein with polymer-based elemental tags. Journal of Analytical Atomic Spectrometry 2014, 29, 1112-1119.

 29. Peng, H. Y., Chen, B. B., He, M., Zhang, Y., Hu, B. Magnetic quantitative immunoanalysis of carcinoembryonic antigen by ICP-MS with mercury labels. Journal of Analytical Atomic Spectrometry 2011, 26, 1217-1223.

 30. Chen, B. B.#, Peng, H. Y.#, Zheng, F., Hu, B., He, M., Zhao, W., Pang, D. W. Immunoaffinity monolithic capillary microextraction coupled with ICP-MS for immunoassay with quantum dot labels. Journal of Analytical Atomic Spectrometry 2010, 25, 1674-1681. (Co-first authors, Cover feature)

 31. Liu, Q. Q., Peng, H. Y. Popowich, A., Tao, J., Uppal, J. S., Yan, X., Beo, D., Le, X. C. Speciation of arsenic–a review of phenylarsenicals and related arsenic metabolites. TrAC Trends in Analytical Chemistry 2018, 104, 171-182.

1. Le, X. C., Zhang, H. Q., Peng, H. Y., Li, X.-F. A microRNA-initiated DNAzyme motor operating in living cells. United States. US 62/422,476. 2016/11/15.

2. Peng, H. Y., Zhang, H. Q., Li, X.-F., Le, X. C. Near-infrared photothermally activated CRISPR/Cas9 genome editing machine. United States. US 62/899,927. 2019/07/25