Environmental chemical biology and health

(a) speciation analysis of arsenic in environmental and biological systems,

(b) ultra-sensitive assays for the detection and imaging of biomolecules, 

(c) construction of target-triggered DNA nanomachine, 

(d) development of CRISPR/Cas9 based nano-technologies for editing specific gene,

(e) development of CRISPR-mediated molecular assays, including diagnostics of COVID-19.

Dr. Hanyong Peng is a professor in Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, China. He received B.Sc. in Chemistry (2008) and Ph.D. in Analytical Chemistry (2014), both from Wuhan University (Wuhan, China). With the support from China Scholarship Council, he pursued his doctoral research (2011-2013) and post-doctoral fellow (2015-2020)  in the Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Canada. 

1. Peng, H. Y., Le, C., Wu, J. J., Li, X. -F., Zhang, H. Q., Le, X. C. A genome editing nanomachine constructed with a clustered regularly interspaced short palindromic repeats system and activated by near-Infrared illumination. ACS Nano 2020, 14, 2817–2826.

2. Wu, J. J.# , Peng, H. Y#. , Lu, X. F., Lai, M., Zhang, H. Q., Le, X. C. Binding-mediated formation of ribonucleoprotein corona for efficient delivery and control of CRISPR-Cas9. Angewandte Chemie International Edition 2020, doi.org/10.1002/anie.202014162. (Co-first authors)

3. Pang, B., Xu, J. Y., Liu, Y. M., Peng, H. Y., Feng, W., Cao, Y. R., Wu, J. J., Xiao, H. Y., Pabbaraju, K., Tipples, G., Joyce, M. A., Saffran, H. A., Tyrrell, D. L., Zhang, H. Q. and Le, X. C. Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR Technology, Analytical Chemistry 2020, 92, 16204–16212.

4. Feng, W.#, Newbigging, A. M.#, Le, C.#, Pang, B.#, Peng, H. Y.#, Cao, Y. Wu, J. J., Abbas, G., Song, J., Wang, D. -B. Cui, M., Tao, J., Tyrrell, D. L., Zhang, X. -E., Zhang, H. Q., Le, X. C. Molecular diagnosis of COVID-19: Challenges and research needs. Analytical Chemistry 2020, 92, 10196–10209. (Co-first authors, Cover feature)

5. Peng, H. Y., Newbigging, A., Reid, M. S., Uppal, J. S., Xu, J., Zhang, H., Le, X. C. Signal amplification in living cells: A review of microRNA detection and imaging. Analytical Chemistry 2020, 92, 292-308.

6. Huang, K., Peng, H. Y., Gao, F., Liu, Q. Q., Lu, X. -F., Shen, Q. R., Le, X. C., Zhao, F. J. Biotransformation of arsenic-containing Roxarsone by an aerobic soil bacterium Enterobacter sp. CZ-1. Environmental Pollution 2019, 247, 482-487.

7. Peng, H. Y., Newbigging, A., Wang, Z., Tao, J., Deng, W., Le, X. C., Zhang, H. Q. DNAzyme-mediated assays for amplified detection of nucleic acids and proteins. Analytical Chemistry 2018, 90, 190-207.

8. Peng, H. Y., Hu, B., Liu, Q. Q., Li, X. -F., Zhang, H. Q., Le, X. C. Methylated phenylarsenical metabolites discovered in chicken liver. Angewandte Chemie International Edition 2017, 56, 6773–6777. (Cover feature)

9. Peng, H. Y., Zhang, H., Li, X. -F., Le, X. C. A microRNA-initiated DNAzyme motor operating in living cells. Nature Communications 2017, 8, 14378. (Highly cited)

10. Yang, Z. L., Peng, H. Y., Lu, X. F., Liu, Q. Q. Huang, R. F., Hu, B., Kachanoski, G., Zuidhof, M. J., Le, X. C. Arsenic metabolites, including N-acetyl-4-hydroxy-m-arsanilic acid, in chicken litter from a roxarsone-feeding study involving 1600 chickens. Environmental Science & Technology 2016, 50, 6737-6743.

11. Liu, Q. Q., Peng, H. Y., Lu, X. F., Zuidhof, M. J., Li, X. -F., Le, X. C. Arsenic species in chicken breast: temporal variations of metabolites, elimination kinetics, and residual Concentrations. Environmental Health Perspectives 2016, 124, 1174-1181.

12. Peng, H. Y., Zhang, N., He, M., Chen, B. B., Hu, B. Multi-wall carbon nanotubes chemically modified silica microcolumn preconcentration/separation combined with inductively coupled plasma optical emission spectrometry for the determination of trace elements in environmental waters. International Journal of Environmental Analytical Chemistry 2016, 96, 212-224.

13. Yan, X. W., Li, J. H., Liu, Q. Q., Peng, H. Y. Popowich, A., Wang, Z. X., Li, X. -F., Le, X. C. p-Azidophenylarsenoxide: A novel arsenical “bait” for the in-situ capture and identification of cellular arsenic-binding proteins. Angewandte Chemie-International Edition 2016, 55, 14051-14057.

14. Zhang, H., Zuehlke, A., Peng, H. Y., Lai, M., Li, X. -F., Le, X. C. Binding-induced DNA nanomachines triggered by proteins and nucleic acids. Angewandte Chemie International Edition 2015, 54, 14326-14330.

15.Peng, H. Y., Reid, M. S., Le, X. C. Consumption of rice and fish in an electronic waste recycling area contributes significantly to tot al daily intake of mercury. Journal of Environmental Sciences 2015, 38, 83-86.

16. Peng, H. Y., Zhang, N., He, M., Chen, B. B., Hu, B. Simultaneous speciation analysis of inorganic arsenic, chromium and selenium in environmental waters by 3-(2-aminoethylamino) propyltrimethoxysilane modified multi-wall carbon nanotubes packed microcolumn solid phase extraction and ICP-MS. Talanta 2015, 131, 266–272. (Highly cited)

17. Peng, H. Y., Hu, B., Liu, Q. Q., Yang, Z. L., Lu, X., Huang, R. F., Li, X. -F., Le X. C. Liquid chromatography combined with atomic and molecular mass spectrometry for speciation of arsenic in chicken liver. Journal of Chromatography A 2014, 1370, 40–49.

18. Peng, H. Y., Jiao, Y., Xiao, X., Chen, B. B., He, M., Liu, Z. R., Zhang, X., Hu, B. Magnetic quantitative analysis for multiplex glycoprotein with polymer-based elemental tags. Journal of Analytical Atomic Spectrometry 2014, 29, 1112-1119.

19. Peng, H. Y., Chen, B. B., He, M., Zhang, Y., Hu, B. Magnetic quantitative immunoanalysis of carcinoembryonic antigen by ICP-MS with mercury labels. Journal of Analytical Atomic Spectrometry 2011, 26, 1217-1223.

20. Chen, B. B.#, Peng, H. Y.#, Zheng, F., Hu, B., He, M., Zhao, W., Pang, D. W. Immunoaffinity monolithic capillary microextraction coupled with ICP-MS for immunoassay with quantum dot labels. Journal of Analytical Atomic Spectrometry 2010, 25, 1674-1681. (Co-first authors, Cover feature)

21. Liu, Q. Q., Peng, H. Y. Popowich, A., Tao, J., Uppal, J. S., Yan, X., Beo, D., Le, X. C. Speciation of arsenic–a review of phenylarsenicals and related arsenic metabolites. TrAC Trends in Analytical Chemistry 2018, 104, 171-182.

22. Chen, J., Zuehlke, A., Deng, B., Peng, H. Y., Hou, X., Zhang, H. A target-triggered DNAzyme motor enabling homogeneous, amplified detection of proteins. Analytical Chemistry 2017, 89, 12888-12895.

23. Moe, B., Peng, H.Y., Lu, X. F., Chen, B.W., Chen, L.W.L., Gabos, S., Li, X. -F., Le, X. C. Comparative cytotoxicity of fourteen trivalent and pentavalent arsenic species determined using real-time cell sensing. Journal of Environmental Sciences 2016, 49, 113-124.

24. Liu, Q.Q., Peng, H. Y., Lu X., Le, X. C. Enzyme-assisted extraction and liquid chromatography mass spectrometry for the determination of arsenic species in chicken meat. Analytica Chimica Acta 2015, 888, 1-9. (Cover feature)

1. Le, X. C., Zhang, H. Q., Peng, H. Y., Li, X.-F. A microRNA-initiated DNAzyme motor operating in living cells. United States. US 62/422,476. 2016/11/15.

2. Peng, H. Y., Zhang, H. Q., Li, X.-F., Le, X. C. Near-infrared photothermally activated CRISPR/Cas9 genome editing machine. United States. US 62/899,927. 2019/07/25