Jian Hao do researches about bio-base chemicals, especially focus on  using Klebsiella pneumoniae as the  cell factory. He have done some researches about the mechanism of 1,3-propaediol  and 2,3-butanediol synthesis by K.  pneumoniae. Besides, He have developed 2-ketogluconic acid, gluconic acid, xylonic  acid, acetoin, isobutanol and 2-ketoisovalerate production by this bacterium.

PhD, Chemical Engineering, Tsinghua University, China 2003-2007.

M.S, Food Engineering, Tianjin Institute of Light Industry, China, 1997-2000.

B.S, Fermentation, Tianjin Institute of Light Industry, China, 1993-1997

2010 to date: Associate Professor, Full Professor, Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, China.

2008-2010: Associate Professor, Department of Environmental and Chemical Engineering Yanshan University, Qinhuangdao China.

2007-2008: Postdoctoral Researcher, Department of Biochemical Engineering, University College London, UK.

2000-2003: Lecture, Department of Environmental and Chemical Engineering Yanshan University, Qinhuangdao China.

   

（1） 1,3-Propanediol production by Klebsiella pneumoniae ∆dhaM∆ptsG∆glpK using glucose and glycerol as co-substrates, Microbial Cell Factories, 2026, 第 9 作者  通讯作者

（2） Improving isobutanol and 2-ketoisovalerate production by reducing by-product synthesis in Klebsiella pneumoniae, Enzyme and Microbial Technology, 2026, 第 8 作者  通讯作者

（3） Mechanism of metabolites distribution between 2,3-butanediol and branched-chain amino acid synthesis pathways in Klebsiella pneumoniae, Journal of Biotechnology, 2025, 第 10 作者  通讯作者

（4） 2-Hydroxyisovalerate production by Klebsiella pneumoniae, ENZYME AND MICROBIAL TECHNOLOGY, 2024, 第 9 作者  通讯作者

（5） Inactivation of hydrogenase-3 leads to enhancement of 1,3-propanediol and 2,3-butanediol production by Klebsiella pneumoniae, Enzyme and Microbial Technology, 2024, 

（6） 克雷伯氏菌细胞工厂, 中国科学:生命科学, 2023, 第 1 作者  通讯作者

（7） 细菌微室, 科学通报, 2023, 第 1 作者  通讯作者

（8） The roles of diol dehydratase from pdu operon on glycerol catabolism in Klebsiella pneumoniae, ENZYME AND MICROBIAL TECHNOLOGY, 2022, 第 10 作者  通讯作者

（9） Improvement of lignocellulosic biomass conversion by optimization of fungal ligninolytic enzyme activity and molasses stillage supplementation, BIOMASS CONVERSION AND BIOREFINERY, 2022, 第 2 作者

（10） Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in l-valine production, WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2022, 第 11 作者  通讯作者

（11） The pyruvate decarboxylase activity of IpdC is a limitation for isobutanol production by Klebsiella pneumoniae, BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS, 2022, 第 12 作者  通讯作者

（12） 以葡萄糖为原料结合生物发酵与化学合成制备酮泛解酸, Ketopantoate production from glucose by combining biological and chemical steps, 过程工程学报, 2022, 第 6 作者  通讯作者

（13） Redirection of the central metabolism of Klebsiella pneumoniae towards dihydroxyacetone production, MICROBIAL CELL FACTORIES, 2021, 第 10 作者  通讯作者

（14） Rcs 信号系统对克雷伯氏肺炎杆菌荚膜合成的影响, Role of Rcs phosphorelay system on capsule synthesis of Klebsiella pneumoniae, 过程工程学报, 2021, 第 6 作者  通讯作者

（15） 1,2-Propanediol production from glycerol via an endogenous pathway of Klebsiella pneumoniae, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2021, 第 10 作者  通讯作者

（16） Ethylene glycol and glycolic acid production by wild-typeEscherichia coli, BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2021, 第 11 作者  通讯作者

（17） 以葡萄糖为原料结合生物发酵与化学合成制备酮泛解酸, 过程工程学报, 2021, 第 6 作者  通讯作者

（18） Ethylene glycol and glycolic acid production from xylonic acid by Enterobacter cloacae, MICROBIAL CELL FACTORIES, 2020, 第 11 作者  通讯作者

（19） Improvement of lignocellulosic biomass conversion by optimization of fungal ligninolytic enzyme activity and molasses stillage supplementation, BIOMASS CONVERSION AND BIOREFINERY, 2020, 第 2 作者

（20） 2,3-Dihydroxyisovalerate production byKlebsiella pneumoniae, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2020, 第 12 作者  通讯作者

（21） 糖酸类化合物的解磷机理研究, Study on mechanism of phosphorus dissolution of sugar acid compounds, 江苏农业科学, 2020, 第 5 作者  通讯作者

（22） Production of 2,3-dihydroxyisovalerate by Enterobacter cloacae, ENZYME AND MICROBIAL TECHNOLOGY, 2020, 第 11 作者  通讯作者

（23） Non-capsulated mutants of a chemical-producing Klebsiella pneumoniae strain., BIOTECHNOLOGY LETTERS, 2018, 第 4 作者  通讯作者

（24） 克雷伯肺炎杆菌2，3-丁二醇合成途径功能基因研究, Study on Functional Genes of 2,3-butanediol Synthesis in Klebsiella Pneumonia, 山东农业大学学报：自然科学版, 2018, 第 5 作者  通讯作者

（25） 基于Red重组系统的阴沟肠杆菌基因重组技术, 食品工业科技, 2018, 第 6 作者  通讯作者

（26） 克雷伯氏肺炎杆菌群体感应系统缺失对合成2，3-丁二醇的影响, Influence of deleting quorum sensing system in Klebsiella pneumoniae on synthesis of 2,3-butanediol, 华中农业大学学报, 2017, 第 5 作者  通讯作者

（27） Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock, JOURNAL OF VISUALIZED EXPERIMENTS : JOVE, 2017, 第 9 作者  通讯作者

（28） The Role of the Pyruvate Acetyl-CoA Switch in the Production of 1,3-Propanediol by Klebsiella pneumoniae, APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2017, 第 9 作者  通讯作者

（29） Isobutanol and 2-ketoisovalerate production by Klebsiella pneumoniae via a native pathway, METABOLIC ENGINEERING, 2017, 第 7 作者  通讯作者

（30） Production of xylonic acid by Klebsiella pneumoniae, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 第 9 作者  通讯作者

（31） Gluconic acid production by gad mutant of Klebsiella pneumoniae, WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 第 8 作者  通讯作者

（32） Inhibition of RecBCD in Klebsiella pneumoniae by Gam and its effect on the efficiency of gene replacement, JOURNAL OF BASIC MICROBIOLOGY, 2016, 第 7 作者  通讯作者

（33） R-acetoin accumulation and dissimilation in Klebsiella pneumoniae, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2015, 第 8 作者  通讯作者

（34） Role of dihydroxyacetone kinases I and II in the dha regulon of Klebsiella pneumoniae, JOURNAL OF BIOTECHNOLOGY, 2014, 第 5 作者  通讯作者

（35） Mechanism of 2,3-butanediol stereoisomer formation in Klebsiella pneumoniae, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2014, 第 5 作者  通讯作者

（36） Two-stage fermentation for 2-Ketogluconic acid production by Klebsiella pneumoniae., JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2014, 第 6 作者  通讯作者

（37） New strategy to improve efficiency for gene replacement in Klebsiella pneumoniae, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2013, 第 5 作者  通讯作者

（38） 2-Ketogluconic acid production by Klebsiella pneumoniae CGMCC 1.6366, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2013, 第 5 作者  通讯作者

（39） Red recombinase assisted gene replacement in Klebsiella pneumoniae, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2012, 第 4 作者  通讯作者

（40） 克雷伯氏肺炎杆菌电击转化条件的优化, Optimization the electroporation parameters of Klebsiella pneumoniae, 中国酿造, 2012, 第 5 作者  通讯作者

（41） 大肠杆菌中1，3-丙二醇氧化还原酶的表达与纯化, Over-expression of Klebsiella pneumoniae 1,3-Propanediol Oxidoreductase Gene dhaT in Escherichia coli and Purification of the Protein, 生物技术, 2011, 第 2 作者  通讯作者

（42） Scale-up of micro-aerobic 1,3-propanediol production with Klebsiella pneumonia CGMCC 1.6366, PROCESS BIOCHEMISTRY, 2009, 第 3 作者

（43） Decrease of 3-hydroxypropionaldehyde accumulation in 1,3-propanediol production by over-expressing dhaT gene in Klebsiella pneumoniae TUAC01., JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2008, 第 1 作者  通讯作者

（44） 酸处理改善1，3-丙二醇发酵液过滤效果的研究, Study of pretreatment with acid to improve filtration effect of 1,3-propanediol fermentation broth, 化学工程, 2008, 第 2 作者

（45） Statistical optimization of culture conditions for 1,3-propanediol by Klebsiella pneumoniae AC 15 via central composite design, BIORESOURCE TECHNOLOGY, 2008, 第 3 作者

（46） 3-Hydroxypropionaldehyde guided glycerol feeding strategy in aerobic 1,3-propanediol production by Klebsiella pneumoniae., JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2008, 第 1 作者  通讯作者

（47） Isolation and characterization of microorganisms able to produce 1,3-propanediol under aerobic conditions, WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2008, 第 1 作者  通讯作者

（48） 产酸克氏杆菌1，3-丙二醇发酵液中2，3-丁二醇的定性和定量分析, Qualitative and Quantitative Analysis of 2,3-Butanediol in 1,3-Propanediol Broth of Klebsiella Oxytoca, 分析化学, 2007, 第 2 作者

（49） pH值对Klebsiella pneumoniae合成1，3-丙二醇中心碳代谢的影响, Effect of pH on Central Carbon Metabolism in 1,3 - Propanediol Production by Klebsiella pneumoniae, 生物技术, 2007, 第 4 作者

（50） 氧对Klebsiella pneumoniae产1，3-丙二醇代谢的影响, Effect of Oxygen on the Metabolism of Klebsiella pneumoniae in 1,3-Propanediol Fermentation, 过程工程学报, 2006, 第 4 作者

（51） Downstream processing of 1,3-propanediol fermentation broth, JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2006, 

（52） 1，3 丙二醇发酵液电渗析法脱盐, Desalination of Fermented Broth Containing 1,3-Propanediol by Electrodialysis, 过程工程学报, 2005, 第 1 作者

（53） 3-羟丙醛对Klebsiella pneumoniae发酵产1，3-丙二醇的影响及其调控, Effect of 3-hydroxypropionaldehyde on 1,3-propanediol fermentation by Klebsiella pneumoniae and regulation for it, 现代化工, 2005, 第 4 作者

（54） Novel route of reactive extraction to recover 1,3-propanediol from a dilute aqueous solution, INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 2005, 第 1 作者

已指导学生

颜薇芝  硕士研究生  085238-生物工程  

魏东  博士研究生  081703-生物化工  

姚尧  硕士研究生  085238-生物工程  

舒琳  硕士研究生  085238-生物工程  

降伟彦  硕士研究生  081703-生物化工  

现指导学生

蔡瑶雨  硕士研究生  086000-生物与医药  

孙少琦  博士研究生  081703-生物化工  

王文琪  硕士研究生  081703-生物化工  

卞心洁  博士研究生  081703-生物化工  

降伟彦  博士研究生  081703-生物化工