基本信息
龚鹏 男 博导 武汉病毒研究所
电子邮件:gongpeng@wh.iov.cn
通信地址:湖北省武汉市武昌区小洪山中区44号
邮政编码:430071

研究领域

        主要从事正链RNA病毒基因组复制机理的研究,解析与复制相关蛋白质及其复合物的三维结构,并结合酶学研究从分子水平上提高对病毒基因组复制的整体认识,从而为抗病毒药物的研发提供重要的理论依据。

招生信息

   
招生专业
071010-生物化学与分子生物学
招生方向
病毒聚合酶催化与调节机制

教育背景

2001-09--2006-02   美国麻州大学阿默斯特校区   博士
1998-09--2001-06   清华大学   硕士
1993-09--1998-06   清华大学   学士

工作经历

   
工作简历
2011-11~现在, 中国科学院武汉病毒研究所, 研究员
2011-03~2011-11,美国科罗拉多州立大学, 研究学者(Research Scientist)
2007-06~2011-02,美国科罗拉多州立大学, 博士后
2006-04~2007-06,美国麻州大学阿默斯特校区, 博士后
社会兼职
2017-06-01-今,湖北省生物化学与分子生物学学会, 常务理事
2016-01-01-今,湖北省晶体学会, 副理事长

出版信息

   
发表论文
[1] Yang, Jieyu, Jing, Xuping, Yi, Wenfu, Li, XiaoDan, Yao, Chen, Zhang, Bo, Zheng, Zhenhua, Wang, Hanzhong, Gong, Peng. Crystal structure of a tick-borne flavivirus RNA-dependent RNA polymerase suggests a host adaptation hotspot in RNA viruses. NUCLEIC ACIDS RESEARCH[J]. 2021, 49(3): 1567-1580, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897508/.
[2] Zhang, BuYu, Liu, Weichi, Jia, Hengxia, Lu, Guoliang, Gong, Peng. An induced-fit de novo initiation mechanism suggested by a pestivirus RNA-dependent RNA polymerase. NUCLEIC ACIDS RESEARCH[J]. 2021, 49(15): 8811-8821, http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000697383500033.
[3] Wu, Jiqin, Wang, Haofeng, Liu, Qiaojie, Li, Rui, Gao, Yan, Fang, Xiang, Zhong, Yao, Wang, Meihua, Wang, Quan, Rao, Zihe, Gong, Peng. Remdesivir overcomes the S861 roadblock in SARS-CoV-2 polymerase elongation complex. CELL REPORTS[J]. 2021, 37(4): 109882-109882, http://dx.doi.org/10.1016/j.celrep.2021.109882.
[4] Shi, Wei, Ye, HanQing, Deng, ChengLin, Li, Rui, Zhang, Bo, Gong, Peng. A nucleobase-binding pocket in a viral RNA-dependent RNA polymerase contributes to elongation complex stability. NUCLEIC ACIDS RESEARCH[J]. 2020, 48(3): 1392-1405, https://www.webofscience.com/wos/woscc/full-record/WOS:000515121900034.
[5] Wang, Quan, Wu, Jiqin, Wang, Haofeng, Gao, Yan, Liu, Qiaojie, Mu, An, Ji, Wenxin, Yan, Liming, Zhu, Yan, Zhu, Chen, Fang, Xiang, Yang, Xiaobao, Huang, Yucen, Gao, Hailong, Liu, Fengjiang, Ge, Ji, Sun, Qianqian, Yang, Xiuna, Xu, Wenqing, Liu, Zhijie, Yang, Haitao, Lou, Zhiyong, Jiang, Biao, Guddat, Luke W, Gong, Peng, Rao, Zihe. Structural Basis for RNA Replication by the SARS-CoV-2 Polymerase. CELL[J]. 2020, 182(2): 417-+, http://dx.doi.org/10.1016/j.cell.2020.05.034.
[6] Meihua Wang, Rui Li, Bo Shu, Xuping Jing, HanQing Ye, Peng Gong. Stringent control of the RNA-dependent RNA polymerase translocation revealed by multiple intermediate structures. NATURE COMMUNICATIONS[J]. 2020, 11(1): http://dx.doi.org/10.1038/s41467-020-16234-4.
[7] Jiqin Wu, HanQing Ye, QiuYan Zhang, Guoliang Lu, Bo Zhang, Peng Gong. A conformation-based intra-molecular initiation factor identified in the flavivirus RNA-dependent RNA polymerase. PLOS PATHOGENS[J]. 2020, 16(5): https://doaj.org/article/0d0d0a1c1e33491aab7fb26012dcc83e.
[8] Liu, Weichi, Shi, Xiaoling, Gong, Peng. A unique intra-molecular fidelity-modulating mechanism identified in a viral RNA-dependent RNA polymerase. NUCLEIC ACIDS RESEARCH[J]. 2018, 46(20): 10840-10854, http://www.corc.org.cn/handle/1471x/2373199.
[9] Zheng, Fengwei, Lu, Guoliang, Li, Ling, Gong, Peng, Pan, Zishu. Uncoupling of Protease trans-Cleavage and Helicase Activities in Pestivirus NS3. JOURNAL OF VIROLOGY[J]. 2017, 91(21): http://www.corc.org.cn/handle/1471x/2373400.
[10] Shu, Bo, Gong, Peng. The uncoupling of catalysis and translocation in the viral RNA-dependent RNA polymerase. RNA BIOLOGY[J]. 2017, 14(10): 1314-1319, http://202.127.146.157/handle/2RYDP1HH/4461.
[11] Kuang, Wenhua, Zhang, Huanyu, Wang, Manli, Zhou, NingYi, Deng, Fei, Wang, Hualin, Gong, Peng, Hu, Zhihong. Three Conserved Regions in Baculovirus Sulfhydryl Oxidase P33 Are Critical for Enzymatic Activity and Function. JOURNAL OF VIROLOGY[J]. 2017, 91(23): http://www.corc.org.cn/handle/1471x/2373360.
[12] Shu, Bo, Gong, Peng. Structural basis of viral RNA-dependent RNA polymerase catalysis and translocation. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2016, 113(28): E4005-E4014, http://www.corc.org.cn/handle/1471x/2374688.
[13] Wu, Jiqin, Liu, Weichi, Gong, Peng. A Structural Overview of RNA-Dependent RNA Polymerases from the Flaviviridae Family. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCESnull. 2015, 16(6): 12943-12957, https://doaj.org/article/64ac7b9c248b43e2af4631867d1d4ae0.
[14] Wu, Jiqin, Lu, Guoliang, Zhang, Bo, Gong, Peng. Perturbation in the Conserved Methyltransferase-Polymerase Interface of Flavivirus NS5 Differentially Affects Polymerase Initiation and Elongation. JOURNAL OF VIROLOGY[J]. 2015, 89(1): 249-261, https://www.webofscience.com/wos/woscc/full-record/WOS:000347176100022.
[15] Li, XiaoDan, Shan, Chao, Deng, ChengLin, Ye, HanQing, Shi, PeiYong, Yuan, ZhiMing, Gong, Peng, Zhang, Bo. The Interface between Methyltransferase and Polymerase of NS5 Is Essential for Flavivirus Replication. PLOS NEGLECTED TROPICAL DISEASES[J]. 2014, 8(5): https://doaj.org/article/631d54570820414ea2e63acc3efb1536.
[16] Guoliang Lu, Peng Gong. Crystal Structure of the Full-Length Japanese Encephalitis Virus NS5 Reveals a Conserved Methyltransferase-Polymerase Interface. Public Library of Science[J]. 2013, 9(8): http://oa.las.ac.cn/oainone/service/browseall/read1?ptype=JA&workid=JA201912010176590ZK.
[17] Gong, Peng, Kortus, Matthew G, Nix, Jay C, Davis, Ralph E, Peersen, Olve B. Structures of Coxsackievirus, Rhinovirus, and Poliovirus Polymerase Elongation Complexes Solved by Engineering RNA Mediated Crystal Contacts. PLOS ONE[J]. 2013, 8(5): https://www.webofscience.com/wos/woscc/full-record/WOS:000319055600004.
[18] Campagnola, Grace, Gong, Peng, Peersen, Olve B. High-throughput screening identification of poliovirus RNA-dependent RNA polymerase inhibitors. ANTIVIRAL RESEARCH[J]. 2011, 91(3): 241-251, http://dx.doi.org/10.1016/j.antiviral.2011.06.006.
[19] Gong Peng. Structure basis for active site closure by the poliovirus RNA-dependent RNA polymerase. Proc Natl Acad Sci USA. 2010, [20] Gong, Peng, Campagnola, Grace, Peersen, Olve B. A quantitative stopped-flow fluorescence assay for measuring polymerase elongation rates. ANALYTICAL BIOCHEMISTRY[J]. 2009, 391(1): 45-55, http://dx.doi.org/10.1016/j.ab.2009.04.035.
[21] Murphy, Michael N, Gong, Peng, Ralto, Kenneth, Manelyte, Laura, Savery, Nigel J, Theis, Karsten. An N-terminal clamp restrains the motor domains of the bacterial transcription-repair coupling factor Mfd. NUCLEIC ACIDS RESEARCH[J]. 2009, 37(18): 6042-6053, [22] Gong, Peng, Martin, Craig T. Mechanism of instability in abortive cycling by T7 RNA polymerase. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2006, 281(33): 23533-23544, http://dx.doi.org/10.1074/jbc.M604023200.
[23] Martin, CT, Esposito, EA, Theis, K, Gong, P, Moldave, K. Structure and function in promoter escape by T7 RNA polymerase. PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, VOL 80null. 2005, 80: 323-+, http://dx.doi.org/10.1016/S0079-6603(05)80008-X.
[24] Theis, K, Gong, P, Martin, CT. Topological and conformational analysis of the initiation and elongation complex of T7 RNA polymerase suggests a new twist. BIOCHEMISTRY[J]. 2004, 43(40): 12709-12715, https://www.webofscience.com/wos/woscc/full-record/WOS:000224371400001.
[25] Gong, P, Esposito, EA, Martin, CT. Initial bubble collapse plays a key role in the transition to elongation in T7 RNA polymerase. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2004, 279(43): 44277-44285, https://www.webofscience.com/wos/woscc/full-record/WOS:000224505600008.
[26] Jing Chen, Yi Chen, Peng Gong, Yang Jiang, YanMei Li, YuFen Zhao. Novel phosphoryl derivatization method for peptide sequencing by electrospray ionization mass spectrometry. Rapid Communications in Mass Spectrometry. 2002, https://www.webofscience.com/wos/woscc/full-record/WOS:000174213700006.

科研活动

   
科研项目
( 1 ) RNA病毒聚合酶的结构与功能研究, 主持, 部委级, 2013-01--2015-12
( 2 ) 重要病毒转录复制复合体的结构功能研究, 主持, 国家级, 2013-01--2017-08
( 3 ) 乙型脑炎病毒非结构蛋白NS5的晶体结构研究, 主持, 国家级, 2014-01--2017-12
( 4 ) 猪瘟病毒NS5B蛋白N端结构域的功能研究, 主持, 国家级, 2017-01--2020-12
( 5 ) 高致病性病毒转录复制过程关键蛋白质机器的功能和干预机制, 参与, 国家级, 2018-06--2022-12
( 6 ) 畜禽重要病原共感染与协同致病机制研究, 主持, 国家级, 2018-06--2020-12
( 7 ) 新型冠状病毒2019-nCoV重要复制加帽酶的工作机制研究, 参与, 国家级, 2020-03--2022-03
( 8 ) 荆门蜱虫病毒聚合酶NSP1的结构及催化特性研究, 主持, 国家级, 2021-01--2024-12

指导学生

已指导学生

舒波  硕士研究生  085238-生物工程  

毕鹏  博士研究生  071010-生物化学与分子生物学  

石伟  博士研究生  071010-生物化学与分子生物学  

逯国亮  博士研究生  071010-生物化学与分子生物学  

舒波  博士研究生  071010-生物化学与分子生物学  

吴继芹  博士研究生  071010-生物化学与分子生物学  

刘伟驰  博士研究生  071010-生物化学与分子生物学  

石晓玲  硕士研究生  085238-生物工程  

杨婕妤  博士研究生  071010-生物化学与分子生物学  

现指导学生

荚恒霞  博士研究生  071010-生物化学与分子生物学  

李瑞  博士研究生  071010-生物化学与分子生物学  

王美华  博士研究生  071010-生物化学与分子生物学  

张步玉  博士研究生  071010-生物化学与分子生物学  

方翔  博士研究生  071010-生物化学与分子生物学  

李思蒙  硕士研究生  100102-免疫学  

王欣雨  博士研究生  071010-生物化学与分子生物学  

钟尧  硕士研究生  086000-生物与医药  

刘顺礼  硕士研究生  086000-生物与医药