主要从事正链RNA病毒基因组复制机理的研究，解析与复制相关蛋白质及其复合物的三维结构，并结合酶学研究从分子水平上提高对病毒基因组复制的整体认识，从而为抗病毒药物的研发提供重要的理论依据。

   

071010-生物化学与分子生物学

病毒聚合酶催化与调节机制

2001-09--2006-02   美国麻州大学阿默斯特校区   博士
1998-09--2001-06   清华大学   硕士
1993-09--1998-06   清华大学   学士

   

2011-11~现在, 中国科学院武汉病毒研究所, 研究员
2011-03~2011-11,美国科罗拉多州立大学, 研究学者(Research Scientist)
2007-06~2011-02,美国科罗拉多州立大学, 博士后
2006-04~2007-06,美国麻州大学阿默斯特校区, 博士后

2017-06-01-今,湖北省生物化学与分子生物学学会, 常务理事
2016-01-01-今,湖北省晶体学会, 副理事长

   

[1] NUCLEIC ACIDS RESEARCH. 2024,   通讯作者  
[2] JOURNAL OF BIOLOGICAL CHEMISTRY. 2023,   通讯作者  
[3] JOURNAL OF BIOLOGICAL CHEMISTRY. 2023,   通讯作者  
[4] PLOS PATHOGENS. 2023,   通讯作者  
[5] Jia, Hengxia, Zhong, Yao, Peng, Chao, Gong, Peng. Crystal Structures of Flavivirus NS5 Guanylyltransferase Reveal a GMP-Arginine Adduct. JOURNAL OF VIROLOGY[J]. 2022, 第 4 作者  通讯作者  96(14): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327709/.
[6] Kuang, Wenhua, Zhang, Huanyu, Cai, Yan, Zhang, Guilin, Deng, Fei, Li, Hailong, Hu, Zhihong, Guo, Yu, Wang, Manli, Zhou, Yiwu, Gong, Peng. Insights into Two-Metal-Ion Catalytic Mechanism of Cap-Snatching Endonuclease of Ebinur Lake Virus in Bunyavirales. JOURNAL OF VIROLOGY[J]. 2022, 第 11 作者  通讯作者  96(6): http://dx.doi.org/10.1128/jvi.02085-21.
[7] Li, Rui, Wang, Meihua, Gong, Peng. Crystal structure of a pre-chemistry viral RNA-dependent RNA polymerase suggests participation of two basic residues in catalysis. NUCLEIC ACIDS RESEARCH[J]. 2022, 第 3 作者  通讯作者  50(21): 12389-12399, http://dx.doi.org/10.1093/nar/gkac1133.
[8] Jiqin Wu, Xinyu Wang, Qiaojie Liu, Guoliang Lu, Peng Gong. Structural basis of transition from initiation to elongation in de novo viral RNA-dependent RNA polymerases. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2022, 第 5 作者  通讯作者  120(1): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9910504/.
[9] Yang, Jieyu, Jing, Xuping, Yi, Wenfu, Li, XiaoDan, Yao, Chen, Zhang, Bo, Zheng, Zhenhua, Wang, Hanzhong, Gong, Peng. Crystal structure of a tick-borne flavivirus RNA-dependent RNA polymerase suggests a host adaptation hotspot in RNA viruses. NUCLEIC ACIDS RESEARCH[J]. 2021, 第 9 作者  通讯作者  49(3): 1567-1580, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897508/.
[10] Zhang, BuYu, Liu, Weichi, Jia, Hengxia, Lu, Guoliang, Gong, Peng. An induced-fit de novo initiation mechanism suggested by a pestivirus RNA-dependent RNA polymerase. NUCLEIC ACIDS RESEARCH[J]. 2021, 第 5 作者  通讯作者  49(15): 8811-8821, http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000697383500033.
[11] Wu, Jiqin, Wang, Haofeng, Liu, Qiaojie, Li, Rui, Gao, Yan, Fang, Xiang, Zhong, Yao, Wang, Meihua, Wang, Quan, Rao, Zihe, Gong, Peng. Remdesivir overcomes the S861 roadblock in SARS-CoV-2 polymerase elongation complex. CELL REPORTS[J]. 2021, 第 11 作者  通讯作者  37(4): 109882-109882, http://dx.doi.org/10.1016/j.celrep.2021.109882.
[12] Shi, Wei, Ye, HanQing, Deng, ChengLin, Li, Rui, Zhang, Bo, Gong, Peng. A nucleobase-binding pocket in a viral RNA-dependent RNA polymerase contributes to elongation complex stability. NUCLEIC ACIDS RESEARCH[J]. 2020, 第 6 作者  通讯作者  48(3): 1392-1405, http://dx.doi.org/10.1093/nar/gkz1170.
[13] Wang, Quan, Wu, Jiqin, Wang, Haofeng, Gao, Yan, Liu, Qiaojie, Mu, An, Ji, Wenxin, Yan, Liming, Zhu, Yan, Zhu, Chen, Fang, Xiang, Yang, Xiaobao, Huang, Yucen, Gao, Hailong, Liu, Fengjiang, Ge, Ji, Sun, Qianqian, Yang, Xiuna, Xu, Wenqing, Liu, Zhijie, Yang, Haitao, Lou, Zhiyong, Jiang, Biao, Guddat, Luke W, Gong, Peng, Rao, Zihe. Structural Basis for RNA Replication by the SARS-CoV-2 Polymerase. CELL[J]. 2020, 第 25 作者  通讯作者  182(2): 417-+, http://dx.doi.org/10.1016/j.cell.2020.05.034.
[14] Meihua Wang, Rui Li, Bo Shu, Xuping Jing, HanQing Ye, Peng Gong. Stringent control of the RNA-dependent RNA polymerase translocation revealed by multiple intermediate structures. NATURE COMMUNICATIONS[J]. 2020, 第 6 作者  通讯作者  11(1): http://dx.doi.org/10.1038/s41467-020-16234-4.
[15] Jiqin Wu, HanQing Ye, QiuYan Zhang, Guoliang Lu, Bo Zhang, Peng Gong. A conformation-based intra-molecular initiation factor identified in the flavivirus RNA-dependent RNA polymerase. PLOS PATHOGENS[J]. 2020, 第 6 作者  通讯作者  16(5): https://doaj.org/article/0d0d0a1c1e33491aab7fb26012dcc83e.
[16] Liu, Weichi, Shi, Xiaoling, Gong, Peng. A unique intra-molecular fidelity-modulating mechanism identified in a viral RNA-dependent RNA polymerase. NUCLEIC ACIDS RESEARCH[J]. 2018, 第 3 作者  通讯作者  46(20): 10840-10854, http://www.corc.org.cn/handle/1471x/2373199.
[17] Shu, Bo, Gong, Peng. The uncoupling of catalysis and translocation in the viral RNA-dependent RNA polymerase. RNA BIOLOGY[J]. 2017, 第 2 作者  通讯作者  14(10): 1314-1319, http://202.127.146.157/handle/2RYDP1HH/4461.
[18] Zheng, Fengwei, Lu, Guoliang, Li, Ling, Gong, Peng, Pan, Zishu. Uncoupling of Protease trans-Cleavage and Helicase Activities in Pestivirus NS3. JOURNAL OF VIROLOGY[J]. 2017, 第 4 作者  通讯作者  91(21): http://www.corc.org.cn/handle/1471x/2373400.
[19] Kuang, Wenhua, Zhang, Huanyu, Wang, Manli, Zhou, NingYi, Deng, Fei, Wang, Hualin, Gong, Peng, Hu, Zhihong. Three Conserved Regions in Baculovirus Sulfhydryl Oxidase P33 Are Critical for Enzymatic Activity and Function. JOURNAL OF VIROLOGY[J]. 2017, 第 7 作者  通讯作者  91(23): http://www.corc.org.cn/handle/1471x/2373360.
[20] Shu, Bo, Gong, Peng. Structural basis of viral RNA-dependent RNA polymerase catalysis and translocation. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2016, 第 2 作者  通讯作者  113(28): E4005-E4014, http://www.corc.org.cn/handle/1471x/2374688.
[21] Wu, Jiqin, Liu, Weichi, Gong, Peng. A Structural Overview of RNA-Dependent RNA Polymerases from the Flaviviridae Family. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES[J]. 2015, 第 3 作者  通讯作者  16(6): 12943-12957, https://doaj.org/article/64ac7b9c248b43e2af4631867d1d4ae0.
[22] Wu, Jiqin, Lu, Guoliang, Zhang, Bo, Gong, Peng. Perturbation in the Conserved Methyltransferase-Polymerase Interface of Flavivirus NS5 Differentially Affects Polymerase Initiation and Elongation. JOURNAL OF VIROLOGY[J]. 2015, 第 4 作者  通讯作者  89(1): 249-261, https://www.webofscience.com/wos/woscc/full-record/WOS:000347176100022.
[23] Li, XiaoDan, Shan, Chao, Deng, ChengLin, Ye, HanQing, Shi, PeiYong, Yuan, ZhiMing, Gong, Peng, Zhang, Bo. The Interface between Methyltransferase and Polymerase of NS5 Is Essential for Flavivirus Replication. PLOS NEGLECTED TROPICAL DISEASES[J]. 2014, 第 7 作者8(5): https://doaj.org/article/631d54570820414ea2e63acc3efb1536.
[24] Guoliang Lu, Peng Gong. Crystal Structure of the Full-Length Japanese Encephalitis Virus NS5 Reveals a Conserved Methyltransferase-Polymerase Interface. PUBLIC LIBRARY OF SCIENCE[J]. 2013, 第 2 作者9(8): 
[25] Gong, Peng, Kortus, Matthew G, Nix, Jay C, Davis, Ralph E, Peersen, Olve B. Structures of Coxsackievirus, Rhinovirus, and Poliovirus Polymerase Elongation Complexes Solved by Engineering RNA Mediated Crystal Contacts. PLOS ONE[J]. 2013, 第 1 作者8(5): https://www.webofscience.com/wos/woscc/full-record/WOS:000319055600004.
[26] Campagnola, Grace, Gong, Peng, Peersen, Olve B. High-throughput screening identification of poliovirus RNA-dependent RNA polymerase inhibitors. ANTIVIRAL RESEARCH[J]. 2011, 第 2 作者91(3): 241-251, http://dx.doi.org/10.1016/j.antiviral.2011.06.006.
[27] Gong Peng. Structure basis for active site closure by the poliovirus RNA-dependent RNA polymerase. Proc Natl Acad Sci USA. 2010, 第 1 作者
[28] Gong, Peng, Campagnola, Grace, Peersen, Olve B. A quantitative stopped-flow fluorescence assay for measuring polymerase elongation rates. ANALYTICAL BIOCHEMISTRY[J]. 2009, 第 1 作者391(1): 45-55, http://dx.doi.org/10.1016/j.ab.2009.04.035.
[29] Murphy, Michael N, Gong, Peng, Ralto, Kenneth, Manelyte, Laura, Savery, Nigel J, Theis, Karsten. An N-terminal clamp restrains the motor domains of the bacterial transcription-repair coupling factor Mfd. NUCLEIC ACIDS RESEARCH[J]. 2009, 第 2 作者37(18): 6042-6053, 
[30] Gong, Peng, Martin, Craig T. Mechanism of instability in abortive cycling by T7 RNA polymerase. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2006, 第 1 作者281(33): 23533-23544, http://dx.doi.org/10.1074/jbc.M604023200.
[31] Martin, CT, Esposito, EA, Theis, K, Gong, P, Moldave, K. Structure and function in promoter escape by T7 RNA polymerase. PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, VOL 80. 2005, 80: 323-+, http://dx.doi.org/10.1016/S0079-6603(05)80008-X.
[32] Theis, K, Gong, P, Martin, CT. Topological and conformational analysis of the initiation and elongation complex of T7 RNA polymerase suggests a new twist. BIOCHEMISTRY[J]. 2004, 43(40): 12709-12715, https://www.webofscience.com/wos/woscc/full-record/WOS:000224371400001.
[33] Gong, P, Esposito, EA, Martin, CT. Initial bubble collapse plays a key role in the transition to elongation in T7 RNA polymerase. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2004, 279(43): 44277-44285, https://www.webofscience.com/wos/woscc/full-record/WOS:000224505600008.
[34] Jing Chen, Yi Chen, Peng Gong, Yang Jiang, YanMei Li, YuFen Zhao. Novel phosphoryl derivatization method for peptide sequencing by electrospray ionization mass spectrometry. RAPID COMMUNICATIONS IN MASS SPECTROMETRY. 2002, 第 3 作者https://www.webofscience.com/wos/woscc/full-record/WOS:000174213700006.

   

（ 1 ） RNA病毒聚合酶的结构与功能研究, 主持, 部委级, 2013-01--2015-12
（ 2 ） 重要病毒转录复制复合体的结构功能研究, 主持, 国家级, 2013-01--2017-08
（ 3 ） 乙型脑炎病毒非结构蛋白NS5的晶体结构研究, 主持, 国家级, 2014-01--2017-12
（ 4 ） 猪瘟病毒NS5B蛋白N端结构域的功能研究, 主持, 国家级, 2017-01--2020-12
（ 5 ） 高致病性病毒转录复制过程关键蛋白质机器的功能和干预机制, 参与, 国家级, 2018-06--2022-12
（ 6 ） 畜禽重要病原共感染与协同致病机制研究, 主持, 国家级, 2018-06--2020-12
（ 7 ） 新型冠状病毒2019-nCoV重要复制加帽酶的工作机制研究, 参与, 国家级, 2020-03--2022-03
（ 8 ） 荆门蜱虫病毒聚合酶NSP1的结构及催化特性研究, 主持, 国家级, 2021-01--2024-12

已指导学生

舒波  硕士研究生  085238-生物工程  

毕鹏  博士研究生  071010-生物化学与分子生物学  

石伟  博士研究生  071010-生物化学与分子生物学  

逯国亮  博士研究生  071010-生物化学与分子生物学  

舒波  博士研究生  071010-生物化学与分子生物学  

吴继芹  博士研究生  071010-生物化学与分子生物学  

刘伟驰  博士研究生  071010-生物化学与分子生物学  

石晓玲  硕士研究生  085238-生物工程  

杨婕妤  博士研究生  071010-生物化学与分子生物学  

现指导学生

荚恒霞  博士研究生  071010-生物化学与分子生物学  

李瑞  博士研究生  071010-生物化学与分子生物学  

王美华  博士研究生  071010-生物化学与分子生物学  

张步玉  博士研究生  071010-生物化学与分子生物学  

方翔  博士研究生  071010-生物化学与分子生物学  

李思蒙  硕士研究生  100102-免疫学  

王欣雨  博士研究生  071010-生物化学与分子生物学  

钟尧  硕士研究生  086000-生物与医药  

刘顺礼  硕士研究生  086000-生物与医药