Viral Bionanotechnology

Sep., 2000—Jul.,2004: Shangdong University, Viotechnology, Bachelor.

Sep., 2004—Jul., 2009: WIV, CAS; Academic Institution, Microbiology, Doctor.

Work Experience

Jul., 2009—Nov., 2009, SINANO, CAS, Assistant Professor

Dec., 2009—Dec., 2011, SINANO, CAS, Postdoctoral

Jan., 2012—Aug., 2013, SINANO, CAS, Associate Professor

Sep., 2013—Present, WIV, CAS; Principle Investigator

1.  杨双宁, 张先恩, 周娟, 李峰. 病毒蛋白冠研究进展. 科学通报,

2.  Feng Li, Lizeng Gao, Fang Yang, Ning Gu,*, XiyunYan,*, Xian-En Zhang*. Nano-synthetic biology for disease diagnosis and treatment. Fundamental Research. 2025. 5(5): 1843-1844 (2025)

3.  Songxin Guo^#, Yi Ru^#, Hui Zhang,Junbiao Xue, Huanan Liu, Dong Men, Zongqiang Cui, Chaochao Shen, Hong Tian, Chun Ma, Jun Gong, Jintian Xu, Dianbing Wang, Rui Gong, Xiaowei Zhang, Heng Rong, Yan-Yi Wang, Chenli Liu, Zhuojun Dai, Shengce Tao*, Jiaoyu Deng*, Haixue Zheng*, Feng Li*, Xian-En Zhang*. An African swine fever virus-specific antibody reactome reveals antigens as potential candidates for vaccine development. Journal of Virology. 2025. 99(9):e0047825

4.  Wei Zhou, Shaojie Ma, Ruimin Gao, Yufu Tang, Hui Zhang, Ao Liang, Mengsi Yang, Chun Ma, Quli Fan*, Xian-En Zhang*, Feng Li*. Assembly of matryoshka-type protein nanocages for compartmentalized oxygen sensing. Nano Letters. 25 (11): 4433–4440 (2025)

5.  Chun Ma, Mengsi Yang, Wei Zhou, Songxin Guo, Hui Zhang, Jun Gong, Xian-En Zhang, Feng Li*. The RNA landscape of in vivo-assembled MS2 virus-like particles as mRNA carriers reveals rna contamination from host viruses. Nano Letters. 25 (8): 3038–3044. (2025)

6.  Songxin Guo, Li Ouyang, Hui Zhang, Ming Li, Wei Zhou, Ao Liang, Lu Wang, Rui Gong, Dianbing Wang, Chenli Liu, Zhuojun Dai, Shengce Tao, Jiaoyu Deng, Guimin Zhang, Xian-En Zhang*, Feng Li*. Construction of an ASFV proteome library via multiple optimization strategies for high-throughput analysis. Acta Biochimica et Biophysica Sinica. DOI:10.3724/abbs.2025125. (2025)

7.  Mengsi Yang^#, Guibo Rao^#, Long Li^#, Linlin Qi, Chun Ma, Hui Zhang, Jun Gong, Bin Wei, Xian-En Zhang, Guosong Chen*, Sheng Cao* and Feng Li*. Transformation of a viral capsid from nanocages to nanotubes and then to hydrogels: Redirected self-assembly and effects on immunogenicity. ACS Nano. 18(21): 13755-13767. (2024)

8.  Xinying Wang, Ruimin Gao, Xuan Wang, Juan Zhou, Xian-En Zhang, Feng Li*. mRNA delivery systems based on protein nanocages: How far can we go? BioDesign Research. 6: 0032. (2024)

9.  Ao Liang, Wei Zhou, Hui Zhang, Juan Zhang, Xian-En Zhang, Ti Fang*, Feng Li*. Effects of individual amino acids on the blood circulation of biosynthetic protein nanocages: toward guidance on surface engineering. Advanced Healthcare Materials. 12(26): 2300502 (2023)(Inside back cover paper)

10. Meixia Du, Feng Li, and Yanwei Hu*. A uniform design method can optimize the combinatorial parameters of antimicrobial photodynamic therapy, including the concentrations of methylene blue and potassium iodide, light dose, and methylene blue’s incubation time, to improve fungicidal effects on candida species. Microorganisms. 11, (10):2557 (2023)

11. 王玹, 万颖, 张先恩, 徐承晨, 李峰. 基于病毒样颗粒的mRNA递送系统研究进展. 科学通报，10.1360/TB-2023-0632 (2023)

12. Quan Cheng, Xuan Wang, Xian-En Zhang, Chengchen Xu*, Feng Li*. Quantitative functionalization of biosynthetic caged protein materials. Qantitative Biology. 11(1): 1-14 (2023)

13. Ti Fang, Chaoqun Li, Ao Liang, Hui Zhang, Fan Zhang, Xian-En Zhang, Yi-Yu Yang*, and Feng Li*. Probing cell membrane integrity using a histone-targeting protein nanocage displaying precisely positioned fluorophores. Nano Research. 16, 894-904 (2023)

14. Xuehong Min, Ming Li, Wenjing Zhang, Run-Hao Li, Zhe Zhang, Pingshan Wang, Weide Su, Feng Li*, Yue Sun*, and Yi Liu*. Pt(II) metallacycles encapsulated by ferritin enable precise cancer combination chemo-photodynamic therapy. Journal of Materials Chemistry B. 11, 1090-1099 (2023)

15. Jingya Tang, Guimin Zhang, Feng Li, Rongyu Zeng, Jin Song, Ghulam Abbas, Mengmeng Cui, Wei Zhang, Xian-En Zhang*, and Dian-Bing Wang*. Two-dimensional protein nanoarray as a carrier of sensing elements for gold-based immunosensing systems. Analytical Chemistry. 94, 26, 9355-9362 (2022)

16. 李峰, 张先恩. 纳米合成生物学：融合创新的新维度. 合成生物学, 3(2): 253-255 (2022)

17. Weide Su, Jingjiang Qiu, Ying Mei, Xian-En Zhang*, Yong He* and Feng Li*. A microfluidic cell chip for virus isolation via rapid screening for permissive cells. Virologica Sinica. 37 (4), 547-557 (2022)

18. Ruimin Gao^#, Huan Tan^#, Shanshan Li^#, Shaojie Ma, Yufu Tang, Kaiming Zhang, Zhiping Zhang, Quli Fan, Jun Yang*, Xian-En Zhang*, Feng Li*. A prototype protein nanocage minimized from carboxysomes with gated oxygen permeability. Proceedings

of the National Academy of Sciences of the United States of America, 119 (5): e2104964119 (2022)

19. Wenjing Zhang, Qingyan Jia, Yibo Teng, Mengsi Yang, Hui Zhang, Xian-En Zhang, Pengfei Wang, Jiechao Ge*, Sheng Cao*, Feng Li*. An ultrastable virus-like particle with a carbon dot core and expanded sequence plasticity. Small. 17 (35), 2101717 (2021)

20. Juan Zhang, Wenjing Zhang, Mengsi Yang, Weiwei Zhu, Ming Li, Ao Liang, Hui Zhang, Ti Fang, Xian-En Zhang and Feng Li*. Passive cancer targeting with a viral nanoparticle depends on the stage of tumorigenesis. Nanoscale. 13, 11334-11342(2021)

21. Ruimin Gao, Xian-En Zhang, Feng Li*. Generation and characterization of self-assembled protein nanocages based on β-carboxysomes in Escherichia coli. Acta Biochimica et Biophysica Sinica. 53, 943–949 (2021)

22. Xuehong Min, Juan Zhang, Run-Hao Li, Fangfang Xia, Shi-Qi Cheng, Ming Li, Weiwei Zhu, Wei Zhou, Feng Li*, and Yue Sun*. Encapsulation of NIR-II AIEgens in virus-like particles for bioimaging. ACS Applied Materials & Interfaces. 13, 15, 17372–17379 (2021)

23. Weiwei Zhu, Ti Fang, Wenjing Zhang, Ao Liang, Hui Zhang, Zhi-Ping Zhang, Xian-En Zhang, Feng Li*. A ROS scavenging protein nanocage for in vitro and in vivo antioxidant treatment. Nanoscale. 13, 4634-4643 (2021)

24. Mariana B. Oliveira, Feng Li, Jonghoon Choi, João F. Mano. Nanomaterials for biomedical applications. Biotechnology Journal.15: 2000574 (2020) (Editorial)

25. 张文静，李明，周维，张先恩，李峰*. 基于病毒组件的纳米材料的自组装合成、功能化及应用. 合成生物学. 1(3):298-318. (2020)

26. Chengchen Xu, Weiwei Zhu, Hanjing Mao, Wenjing Zhang, Gen-Quan Yin*, Xian-En Zhang*, Feng Li*. Switch from polymorphic to homogenous self-assembly of virus-like particles of simian virus 40 through double-cysteine substitution. Small. 16(45): 2004484 (2020)

(Back cover paper)

27. Feng Li*, Dianbing Wang, Juan Zhou, Dong Men and Xian-En Zhang*. Design and biosynthesis of functional protein nanostructures. Science China Life Sciences. 63: 1142–1158 (2020)

28. Michele Dei Cas, Gabriella Roda, Feng Li, and Francesco Secundo*. Functional lipids in autoimmune inflammatory diseases. International Journal of Molecular Sciences. 21: 3074 (2020)

29. Guochang Liu, Ming Fu, Feng Li, Wen Fu, Zhang Zhao, Huimin Xia*, and Yuqing Niu*. Tissue-engineered PLLA/gelatine nanofibrous scaffold promoting the phenotypic expression of epithelial and smooth muscle cells for urethral reconstruction. Materials Science and

Engineering: C. 111: 110810 (2020)

30. Xuehong Min, Ti Fang, Lingling Li, Chaoqun Li, Zhi-Ping Zhang, Xian-En Zhang and Feng Li*. AIE nanodots scaffolded by mini-ferritin protein for cellular imaging and photodynamic therapy. Nanoscale. 12, 2340-2344 (2020)

31. Ti Fang, Weiwei Zhu, Chaoqun Li, Fan Zhang, Ding Gao, Zhi-Ping Zhang, Ao Liang, Xian-En Zhang* and Feng Li*. Role of Surface RGD Patterns on Protein Nanocages in Tumor Targeting Revealed Using Precise Discrete Models. Small. 15: 1904838 (2019)

32. Tingting Wang^#, Guang Li, Dianbing Wang*, Feng Li, Dong Men, Tao Hu, Yan Xi and Xian-En Zhang*. Quantitative profiling of integrin αvβ3 on single cells with quantum dot labeling to reveal the phenotypic heterogeneity of glioblastoma. Nanoscale, 11:18224-18231 (2019)

33. Lingling Li, Chengchen Xu, Wenjing Zhang, Francesco Secundo, Chunyan Li, Zhi-Ping Zhang, Xian-En Zhang* and Feng Li*. Cargo-Compatible Encapsulation in Virus-Based Nanoparticles. NanoLetters. 19(4): 2700-2706 (2019)

34. Jigang Wang, Ti Fang, Ming Li, Wenjing Zhang, Zhiping Zhang, Xian-En Zhang* and Feng Li*. Intracellular delivery of peptide drugs using viral nanoparticles of bacteriophage P22: covalent loading and cleavable release. Journal of Materials Chemistry B. 6, 3716-3726 (2018)

35. Wenjing Zhang, Xian-En zhang*, Feng Li*. Virus-based nanoparticles of simian virus 40 in the field of nanobiotechnology. Biotechnology Journal. 13(6): 1700619. (2018)

36. Wenjing Zhang^#, Chengchen Xu^#, Gen-Quan Yin, Xian-En Zhang, Qiangbin Wang*, Feng Li*. Encapsulation of Inorganic Nanomaterials inside Virus-Based Nanoparticles for Bioimaging. Nanotheranostics.1(4):358-368 (2017)  (^#equal contribution)

37. Wenjing Zhang, Zhi-Ping Zhang, Xian-En Zhang*, Feng Li*. Reaction inside a Viral Protein Nanocage:Mineralization on a Nanoparticle Seed after Encapsulation via Self-Assembly. Nano Research. 10(10):3285-3294 (2017)

38. 李峰*，门冬，王殿冰，张先恩*. 生物大分子自组装合成多维纳米生物结构与器件. 生物工程学报. 33 (3): 404-421(2017)

39. Chunyan Li, Feng Li,* Yejun Zhang, Wenjing Zhang, Xian-En Zhang, and Qiangbin Wang*. Real-Time Monitoring Surface Chemistry-Dependent In Vivo Behaviors of Protein Nanocages via Encapsulating an NIR-II Ag₂S Quantum Dot. ACS Nano 9(12): 12255-12263. (2015)

40. Lingzhi Ma, Feng Li,* Ti Fang, Jianting Zhang, and Qiangbin Wang*. Controlled Self-Assembly of Proteins into Discrete Nanoarchitectures Templated by Gold Nanoparticles via Monovalent Interfacial Engineering. ACS Applied Materials & Interfaces 7(20):11024-11031. (2015)

41. Feng Li and Qiangbin Wang*. Fabrication of nanoarchitectures templated by virus-based nanoparticles: strategies and applications. Small 10(2): 230-245.(2014) (Back-cover paper; invited review)

42. Feng Li^#, Huiling Chen^#, Lingzhi Ma, Kun Zhou, Zhi-Ping Zhang, Chun Meng*, Xian-En Zhang* and Qiangbin Wang*. Insights into stabilization of a viral protein cage in templating complex nanoarchitectures: roles of disulfide bonds. Small10(3): 536-543. (2014) (^#共同第一作者)

43. Feng Li, Huiling Chen, Yejun Zhang, Zhong Chen, Zhi-Ping Zhang, Xian-En Zhang* and Qiangbin Wang*. Three-dimensional gold nanoparticle clusters with tunable cores templated by a viral protein scaffold. Small 8(24): 3832-3838. (2012)

44. Feng Li, Ding Gao, Xiaomin Zhai, Yanhua Chen, Tao Fu, Dongmin Wu, Zhi-Ping Zhang, Xian-En Zhang* and Qiangbin Wang*. Tunable, discrete, three-dimensional hybrid nanoarchitectures. Angewandte Chemie International Edition 123(18): 4288-4291. (2011)

45. Feng Li, Yanhua Chen, Huiling Chen, Wei He, Zhi-Ping Zhang, Xian-En Zhang* and Qiangbin Wang*. Monofunctionalization of protein nanocages. Journal of the American Chemical Society 133(50): 20040-20043. (2011)

46. Feng Li^#, Ke Li^#, Zong-Qiang Cui, Zhi-Ping Zhang, Hong-Ping Wei, Ding Gao, Jiao-Yu Deng and Xian-En Zhang*. Viral coat proteins as flexible nano-building-blocks for nanoparticle encapsulation. Small 6(20): 2301-2308. (2010) (^#共同第一作者)

47. Feng Li, Zhi-Ping Zhang, Jun Peng, Zong-Qiang Cui, Dai-Wen Pang, Ke Li, Hong-Ping Wei, Ya-Feng Zhou, Ji-Kai Wen and Xian-En Zhang*. Imaging viral behavior in mammalian cells with self-assembled capsid-quantum-dot hybrid particles. Small 5(6): 718-726. (2009) (Inside-cover paper; highlighted by Nature China)

48. Kun Zhou, Feng Li, Gaole Dai, Chun Meng and Qiangbin Wang*. Disulfide bond: dramatically enhanced assembly capability and structural stability of tobacco mosaic virus nanorods. Biomacromolecules 14(8): 2593-2600. (2013)

49. Yan Zhang, Yejun Zhang, Guosong Hong, Wei He, Kun Zhou, Kai Yang, Feng Li, Guangcun Chen, Zhuang Liu, Hongjie Dai and Qiangbin Wang*. Biodistribution, pharmacokinetics and toxicology of Ag₂S near-infrared quantum dots in mice. Biomaterials 34(14): 3639-3646. (2013)

50. Ding Gao, Zhi-Ping Zhang, Feng Li, Dong Men, Jiao-Yu Deng, Hong-Ping Wei, Xian-En Zhang* and Zong-Qiang Cui*. Quantum dot-induced viral capsid assembling in dissociation buffer. International journal of nanomedicine 8:2119. (2013)

51. Yan Zhang, Guosong Hong, Yejun Zhang, Guangcun Chen, Feng Li, Hongjie Dai* and Qiangbin Wang*. Ag₂S quantum dot: A bright and biocompatible fluorescent nanoprobe in the second near-infrared window. ACS Nano 6(5): 3695-3702. (2012)

52. Tingjuan Wang, Zhi-ping Zhang, Ding Gao, Feng Li, Hong-ping Wei, Xiaosheng Liang, Zong-qiang Cui* and Xian-En Zhang*. Encapsulation of gold nanoparticles by simian virus 40 capsids. Nanoscale 3(10): 4275-4282. (2011)

53. Yaping Du, Bing Xu, Tao Fu, Miao Cai, Feng Li, Yan Zhang and Qiangbin Wang*. Near-infrared photoluminescent Ag₂S quantum dots from a single source precursor. Journal of the American Chemical Society 132(5): 1470-1471. (2010)

54. Miao Cai, Feng Li, Yan Zhang and Qiangbin Wang*. One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection. Nano Research 3(8): 557-563. (2010)

55. 李峰, 董博华, 张叶俊, 兰祥, 李春炎, 王强斌*. 无机半导体纳米晶光学性质的设计、调控及其生物医学应用. 中国科学：化学. 44(2): 180-190. (2014)

（ 1 ） 一种带纯化标签的P22 病毒样颗粒的制备及其应用, 发明专利, 2025, 第 1 作者, 专利号: 202511507803.0

（ 2 ） 一种非洲猪瘟病毒全蛋白质芯片及应用, 发明专利, 2024, 第 2 作者, 专利号: CN119224310A

（ 3 ） 来自于李斯特菌的Dps蛋白纳米笼在制备治疗脓毒症的药物中的应用, 发明专利, 2024, 第 2 作者, 专利号: CN117582487A

（ 4 ） 一种跨膜的饥饿诱导的DNA结合蛋白及应用, 发明授权, 2023, 第 1 作者, 专利号: CN110283251B

（ 5 ） 一种用于病毒分离培养的微流控细胞芯片, 实用新型, 2022, 第 1 作者, 专利号: CN216237079U

（ 6 ） 微流控细胞芯片和基于该细胞芯片的病毒分离培养方法, 发明专利, 2021, 第 1 作者, 专利号: CN113637582A

（ 7 ） 一种在蛋白纳米笼内部包装外源物质的方法, 发明专利, 2017, 第 1 作者, 专利号: CN107164339A

（ 8 ） 不对称病毒纳米颗粒的制备方法, 发明专利, 2012, 第 2 作者, 专利号: CN102321590A

（ 9 ） 纳米生物机器人及其应用, 发明专利, 2010, 第 4 作者, 专利号: CN101744645A

    Viral Bionanotechnology

已指导学生

吴超云  硕士研究生  071005-微生物学  

张文静  硕士研究生  085238-生物工程  

高瑞敏  博士研究生  071010-生物化学与分子生物学  

朱伟伟  博士研究生  071010-生物化学与分子生物学  

张文静  博士研究生  071010-生物化学与分子生物学  

李玲玲  博士研究生  071010-生物化学与分子生物学  

毛晗静  硕士研究生  085238-生物工程  

李超群  硕士研究生  085238-生物工程  

苏炜德  博士研究生  071010-生物化学与分子生物学  

张娟  博士研究生  071010-生物化学与分子生物学  

李明  硕士研究生  071010-生物化学与分子生物学  

王继刚  博士研究生  071005-微生物学  

郭颂欣  博士研究生  071010-生物化学与分子生物学  

马春  博士研究生  071010-生物化学与分子生物学  

杨梦思  博士研究生  071005-微生物学  

王露  硕士研究生  085238-生物工程  

梁澳  博士研究生  071010-生物化学与分子生物学  

周维  博士研究生  071010-生物化学与分子生物学  

王昕颖  硕士研究生  086000-生物与医药  

现指导学生

周娟  博士研究生  071010-生物化学与分子生物学  

龚俊  博士研究生  071010-生物化学与分子生物学  

唐咏捷  博士研究生  071010-生物化学与分子生物学  

陈元雪  博士研究生  071005-微生物学  

孟瑶  博士研究生  071010-生物化学与分子生物学  

曾怡敏  硕士研究生  086000-生物与医药  

付江凤  硕士研究生  086000-生物与医药  

李昭玥  博士研究生  071010-生物化学与分子生物学  

饶海月  博士研究生  071010-生物化学与分子生物学  

彭鹏  博士研究生  071010-生物化学与分子生物学  

陈驰  硕士研究生  071010-生物化学与分子生物学  

杨双宁  硕士研究生  071010-生物化学与分子生物学