​Our research spans a broad range of Microbiology, Biochemistry, and Microfluidics. We use microfluidic droplets for high-throughput culturing and screening of single cells in nanoliter to picoliter volumes. We are developing automated workflows and devices for large scale single-cell isolation, cultivation, interaction and sequencing. We want to provide better approaches to improve the cultivation of microbial samples, to isolate and study rare microbes and extremophiles from the environments, to study the diversity, functions and evolution of complex microbial communities, and to advance evaluation and applications of microbial resources. Our current ongoing projects include:

1. Single-cell isolation, cultivation and sorting using high-throughput droplet microfluidic technologies.
2. single cell whole genome amplification of uncultured microorganisms.
3. digial PCR and applications.
4. Bacterial chemotaxis analysis and separation.
5. isolation, identification and antimicrobial susceptibility testing of pathogens in clinical and environmental samples.

​Department of Chemistry, Zhejiang University, BSc in Chemistry, 06/1998  
Institute of Microanalytical Systems, Zhejiang University, PhD in Chemistry with Professor Qun Fang and Professor Zhao-Lun Fang, 09/2002 - 07/2007

​Postdoctoral Fellow, University of Chicago, (with Professor Rustem F. Ismagilov), 09/2007 - 08/2011 

Professor, Department of Chemistry, Renmin Univeristy, 09/2011 - 11/2013  
Professor, Institute of Microbiology, Chinese Adademy of Sciences, 11/2013 -

​The Recruitment Program for Young Professionals of the Chinese Government (中组部第二批“青年****”), 2012

Hundred Talents Program of the Chinese Academy of Sciences (中国科学院“****”), 2012
Collegiate Inventors Competition Finalists (with Dr. Feng Shen), Washington DC, USA, 10/2010

1. ang, Z.Y.; Yu, T.; Gong, L.F.; Qi, H.B.; Sun, Y.; Wu, X.L.; Wang, Z.R.; Wang, S.; Jiang, L.; Dai, X.; Xiao, X.; Zheng, X.W.; Huang, L.; Li, G.Y.; Dong, H.L.; Wang, H.L.; Shao, Z.Z.; Du, W.B.*, High-throughput single-cell cultivation under high pressure unlocks the deep-sea microbial dark matter, Under review.
2. Liu, Y.F.; Zhao, H.W.; Cao, X.Y.; Jiang, S.Z.; Fu, J.; Xue, J.J.; Li, C.M.; Xu, Z.R.; Li, M.; Du, W.B.*, Rapid and quantitative analysis of bacteriophage infectivity via fully-automated droplet digital PCR, 2026, Under review.
3. 余治鸿; 乔雨歆; 王春晓; 汪之一; 何湘伟*; 杜文斌*, 基于荧光纳米探针的聚对苯二甲酸乙二醇酯水解酶高通量液滴微流控筛选, 生物工程学报, 2026, Accepted.
4. Han, W.W.#; Cheng, X.#; Luo, Y.M.; Ma, A.T.; Xu, X.Q.; Du, W.B.; Wang, Y.P.*, On-demand recession of quaternary ammonium biocides for combating multidrug resistance, Biomaterials, 2026, 333, 124218.
5. Li, Y.C.; Cao, Z.M.; Xu, Y.B.; Li, C.M.; Ye, S.; Wu, X.L.; Wang, Z.R.; Chen, H.P.; Liu, H.C.; Hu, X.Y.; Wang, T.; Zeng, W.Q.; Wang, Q.; Guo, L.; Du, W.B.*, High-fidelity bioassembly of organoids and spheroids using inertial droplet microfluidics for precision oncology and tumor microenvironment modeling, Microsystems & Nanoengineering, 2026, 12(1), 152.
6. Hu, Y.T.; Song, J.C.; Li, Y.C.; Wu, X.L.; Wang, Z.Y.; Lan, Y.; Xiang, N.; Xu, Z.R.*; Du, W.B.*, Microfluidic Fluorescence-Activated Cell Sorting via Gradient Dielectrophoresis, Analytical Chemistry, 2026, 98(13), 9523-9530.
7. ang, Z.R.; Cheng, X.; Xu, Y.B.; Wang, Z.Y.; Ma, L.Y.; Li, C.M.; Jiang, S.Z.; Li, Y.C.; Guo, S.L.; Du, W.B.*, Unveiling Neonatal Pneumonia Microbiome by High-Throughput Sequencing and Droplet Culturomics, Genomics, Proteomics & Bioinformatics, 2025, 23(6), qzaf047.
8. Sun, W.J.; Li, C.M.; Wu, X.L.; Cheng, X.; Li, Y.C.; Yang, L.-M.; Liu, Y. Guo, S.L.; Ma, L.Y.; Qiu, H.W.*; Du, W.B.*, Point-of-care detection of Mycobacterium in bovine feces using a portable real-time loop-mediated isothermal amplification system, Sensors and Actuators B: Chemical, 2025, 440, 137857.
9. Chen, H.H.; Zheng, F.F.; Feng, X.; Huang, Z.J.; Yang, W.; Zhang, C.L.; Du, W.B.; Makarova, K.S.; Koonin, E.K.; Zeng, Z.R.*, Engineering archaeal membrane-spanning lipid GDGT biosynthesis in bacteria: implications for early life membrane transformations, mLife, 2025, 4(2), 193-204.
10. Wang, J.Y.; Geng, Y.R.; Guo, J.; Peng, J.H.; Xu, H.; Zhao, B.Q.; Huang, S.Y.; Qin, M.; Du, W.B.*; Tian, J.*, The subgingival microbial composition in health and periodontitis with different probing depths, Microorganisms, 2025, 13(4), 930.
11. Wang, Z.Y.; Sun, Y.; Wang, H.L.; Yun, J.L.*; Du, W.B.*, Metagenome-assembled genome of a novel Pseudoalteromonas species from South Mid-Atlantic Ridge deep-sea water suggests potential for chitin degradation, Microbiology Resource Announcements, 2025, 14(5), e00189-25.
12. Xu, Y.B.#; Wang, Z.Y.#; Li, C.M.; Tian, S.Q.; Du, W.B.*, Droplet microfluidics: unveiling the hidden complexity of the human microbiome, Lab On a Chip, 2025, 25, 1128-1148 (Critical Review).
13. Shen, W.L.; Wang, D.R.; Li, J.T.; Liu, Y.; Wang, Y.Z.; Yang, X.S.; Peng, X.; Xie, B.L.; Su, L.; Wei, Z.Y.; He, Q.; Wang, Z.Y.; Feng, K.; Du, W.B.*; Deng, Y.*, Developing a microfluidic-based epicPCR reveals diverse potential hosts of mcrA gene in marine cold seep, mLife, 2025, 4(1), 70-82.
14. Li, C.M.; Kang, N.; Ye, S.; Huang, W.H.; Wang, X.; Wang, C.; Li, Y.C.; Liu, Y.F.; Lan, Y.; Ma, L.; Zhao, Y.H.; Han, Y.; Fu, J.; Shen, D.H.; Dong, L.H.*; Du, W.B.*, All-in-One OsciDrop Digital PCR System for Automated and Highly Multiplexed Molecular Diagnostics, Advanced Science, 2024, 11(21), 2309557.
15. Tian, J.; Zhao, B. Q.; Wang, J. Y.; Du, W. B.; Ma, W.L.; Xia, B.; Xu, H.; Chen, T.; He, X.S.*; Qin, M.*, The short-term impact of comprehensive caries treatment on the supragingival microbiome of severe early childhood caries, International Journal of Paediatric Dentistry, 2024, 34(5), 505-515.
16. Wu, X.L.; Xie, B.L.; Qiao, Y.X.*; Yuan, S.; Du, W.B.*, μMET: A Novel Reusable Microfluidic Chip for Precision Microbial Enumeration Tests, Analytical Chemistry, 2024, 96(2), 630-635.
17. Shan, M.J.; Xiao, M.; Xu, J.Y.; Sun, W.; Wang, Z.R.; Du, W.B.; Liu, X.Y.; Nie, M.; Wang, X.; Liang, Z.Y.; Liu, H.; Hao, Y.; Xia, Y.J.; Zhu, L.; Song, K.X.; Feng, C.; Meng, T.; Wang, Z.; Cao, W.F.; Wang, L.; Zheng, Z.; Wang, Y.B.*; Huang, Y.S.*, Multi-Omics Analyses Reveal Bacteria and Catalase Associated with Keloid Disease, eBioMedicine, 2024, 99, 104904.
18. 雨庆; 彭显; 任彪; 闫福华; 潘亚萍; 陈峰; 杜文斌; 刘建国; 冯强; 杨德琴; 黄晓晶; 潘乙怀; 黄正蔚; 丁佩惠; 章可可; 刘红霞; 周学东*, 口腔微生物译名 (2023版), 中华口腔医学杂志, 2023, 58, 1045-1055.
19. Yang, J.R.; Yun, J.L.; Liu, X.Z.; Du, W.B.; Xiang, M.C.*, Niche and ecosystem preference of earliest diverging fungi in soils, Mycology, 2023, 14, 1-17.
20. 杜文斌; 崔丽; 陈栋炜; 乔雨歆; 杨凯, 单细胞技术 (第21章); 土壤生物学, 朱永官; 褚海燕主编, 高等教育出版社, 2023, pp.315-327.
21. Xie, B.L.; Wang, J.; Nie, Y.; Tian, J.; Wang, Z.R.; Chen, D.W.; Hu, B.Y.; Wu, X.-L.; Du, W.B.*, Type IV Pili Trigger Episymbiotic Association of Saccharibacteria with Its Bacterial Host, Proceedings of the National Academy of Sciences of the United States of America, 2022, 119(49), e2215990119.
22. Zhang, J.L.; Yun, J.L.; Yue, L.; Du, W.B.*; Liang, Y.H.*, Distinctive Microbiota Distribution from Healthy Oral to Post-treatment Apical Periodontitis, Frontiers in Cellular and Infection Microbiology, 2022, 12, 980157.
23. Chen, D.W.; Nie, M.Y.; Tang, W.; Zhang, Y.W.; Qiao, Y.X.; Yun, J.L.; Wang, J.; Lan, Y.; Chen, Y.H.*; Du, W.B.*, Whole Lifecycle Observation of Single-spore Germinated Streptomyces Using a Nanogap-stabilized Microfluidic Chip, mLife, 2022, 1(3), 341-349.
24. Feng H.C.; Lv Y.; Krell T.; Fu R.X.; Liu, Y.P.; Xu, Z.H.; Du, W.B.; Shen, Q.R.; Zhang, N.; Zhang, R.F.*, Signal binding at both modules of its dCache domain enables the McpA chemoreceptor of Bacillus velezensis to sense different ligands, Proceedings of the National Academy of Sciences of the United States of America, 2022, 119(29), e2201747119.
25. Ye, S.#; Li, C.M.#; Zheng, X.*; Huang, W.H.; Tao, Y.; Yu, Y.H.; Yang, L.M.; Lan, Y.; Ma, L.*; Bian, S.T.*; Du, W.B.*, OsciDrop: A Versatile Deterministic Droplet Generator, Analytical Chemistry, 2022, 94, 6, 2918-2925.
26. Qiao, Y.X.; Hu, R.; Chen, D.W.; Wang, L.; Wang, Z.Y.; Yu, H.Y.; Fu, Y.; Li, C.L.; Dong, Z.Y.; Weng, Y.-X.*; Du, W.B.*, Fluorescence-Activated Droplet Sorting of PET Degrading Microorganisms, Journal of Hazardous Materials, 2022, 424, 127417.
27. 倍瑜; 徐冰雪; 贠娟莉; 陈栋炜; 杜文斌, 基于液滴微流控的微生物单细胞高通量培养分选技术 (第12章第二节); 中国土壤微生物组, 朱永官; 沈仁芳主编, 浙江大学出版社, 2021, pp. 563-577.
28. Hu, B.Y.; Ye, S.; Chen, D.W.; Xie, B.L.; Hu, R.; Qiao, Y.X.; Yu, Y.H.; Yu, H.Y.; Zheng, X.; Lan, Y.; Du, W.B.*, Tunable and Contamination-free Injection with Microfluidics by Stepinjection, Analytical Chemistry, 2021, 93(39), 13112-13117.
29. Yun, J.L.; Crombie, A.T.; Ul Haque, F.M; Cai, Y.F.; Zheng, X.W.; Wang, J.; Jia, Z.J.; Murrell, J.C.; Wang, Y.F.*; Du, W.B.*, Revealing the community and metabolic potential of active methanotrophs by targeted metagenomics in the Zoige wetland of the Tibetan Plateau, Environmental Microbiology, 2021, 23(11), 6520-6535.
30. Hu, B.Y.; Xu, P.; Ma, L.; Chen, D.W.; Wang, J.; Dai, X.; Huang, L.; Du, W.B.*, One cell at a time: droplet-based microbial cultivation, screening, and sequencing, Marine Life Science & Technology, 2021, 3, 169-188.
31. Cui, Y.L.; Chen, Y.C.; Liu, X.Y.; Dong, S.J.; Tian, Y.E.; Qiao, Y.X.; Mitra, R.; Han, J.; Li, C.L.; Han, X.; Liu, W.D.; Chen, Q.; Wei, W.Q.; Wang, X.; Du, W.B.; Tang, S.Y.; Xiang, H.; Liu, H.Y.; Liang, Y.; Houk, K.; Wu, B.*, Computational redesign of a PETase for plastic biodegradation under ambient condition by the GRAPE strategy, ACS Catalysis, 2021, 11, 1340-1350.
32. 张婧琳; 胡然; 靳增军; 杜文斌*; 梁宇红*, 基于微流控的生物被膜研究进展及口腔微生态系统应用展望, 微生物学报, 2021, 61(5), 1091-1105.
33. 阮楚晋; 郑小伟; 王丽; 王铱; 朱雅新; 王剑; 贠娟莉; 董志扬; 陆祖军; 黄英; 杜文斌; 黄力; 戴欣*, 基于流式细胞仪高通量分选的深海微生物单细胞培养, 微生物学报, 2021, 61(04): 816-827.
34. 张思琦; 王剑; 兰英; 贠娟莉; 陈建; 何湘伟*; 杜文斌*, 抗菌活性放线菌的界面微滴移液互作分离筛选方法, 微生物学通报, 2021, 48(1), 325-335.
35. Zheng, X.W.; Liu, W.; Dai, X.; Zhu, Y.X.; Wang, J.F.; Zhu, Y.Q.; Zheng, H.J.; Huang, Y.; Dong, Z.Y.; Du, W.B.; Zhao, F.Q.; Huang, L.*, Extraordinary diversity of viruses in deep-sea sediments as revealed by metagenomics without prior virion separation, Environmental Microbiology, 2021, 23(2):728-743.
36. 段云峰; 王升跃; 陈禹保; 杨瑞馥; 李后开; 朱怀球; 童贻刚; 杜文斌; 付钰; 胡松年; 王军; 辛玉华; 赵方庆; 鲍一明; 张雯; 曾明; 牛海涛; 周欣; 李岩; 崔生辉; 袁静; 李俊桦; 王加义; 刘东来; 倪铭; 孙青; 邓晔; 朱宝利*, 微生物组测序与分析专家共识, 生物工程学报, 2020, 36(12), 2516-2524.
37. Ruan, C.J.; Wang, J.; Zheng, X.W.; Song, L.; Zhu, Y.X.; Huang, Y.; Lu, Z.J.; Du, W.B.; Huang, L.; Dai, X.*, Halovulum marinum sp. nov., isolated from deep seawater of the Northwest Indian Ocean, and emended description of the genus Halovulum, International Journal of Systematic and Evolutionary Microbiology, 2020,70(8), 4523-4530.
38. Wang, J.; Ruan, C.-J.; Song, L.; Zhu, Y.-X.; Li, A.; Zheng, X.-W.; Wang, L.; Lu, Z.-J.; Huang, Y.; Du, W.B.; Zhou, Y.G.; Huang, L.; Dai, X.*, Gimesia benthica sp. nov., a novel planctomycete isolated from a deep-sea water sample of the Northwest Indian Ocean, International Journal of Systematic and Evolutionary Microbiology, 2020, 70(7), 4384-4389.
39. Tao, Y.; Yun, J.L.; Wang, J.; Xu, P.; Li, C.M.; Liu, H.T.; Lan, Y.*; Pan, J.Z.*; Du, W.B.*, High-Performance Detection of Mycobacterium bovis in Milk Using Digital LAMP, Food Chemistry, 2020, 327, 126945.
40. Liu, Y.Y.; Xu, J.J.; Tao, Y.; Fang, T.; Du, W.B.; Ye, A.P.*, Rapid and accurate identification of marine microbes with single-cell Raman spectroscopy, Analyst, 2020, 145, 3297-3305.
41. Qu, H.J.; Yu, M.C.; Du, W.B.; Xu, L.; Lv, W.Y.; Shen, F.*, Slip molding for precision fabrication of microparts, Langmiur, 2020, 36, 2, 585-590.
42. Hu, B.Y.; Xu, B.X.; Yun, J.L.*; Wang, J.; Xie, B.L.; Li, C.M.; Yu, Y.H.; Lan, Y.; Zhu, Y.X.; Dai, X.; Huang, Y.; Huang, L.; Pan, J.Z.*; Du, W.B.*, High-throughput Single-cell Cultivation Reveals Underexplored Rare Biosphere in Deep-sea Sediments Along the Southwest Indian Ridge, Lab On a Chip, 2020, 20, 363-372.
43. Yun, J.L.#; Zheng, X.W.#; Xu. P.#; Zheng, X.; Xu, J.Y.; Cao, C.; Fu, Y.S.; Xu, B.X.; Dai, X.; Wang, Y.; Liu, H.T.; Yi, Q.L.; Zhu, Y.X.; Wang, J.; Wang, L.; Dong, Z.Y.; Huang, L.*; Huang, Y.Y.*; Du, W.B.*, Interfacial Nanoinjection-based Nanoliter Single-cell Analysis, Small, 2020, 16, 1903739.
44. Liu, Y.P.; Feng, H.C.; Fu, R.X.; Zhang, N.; Du, W.B.; Shen, Q.R.; Zhang, R.F.*, Induced root-secreted D-galactose functions as a chemoattractant and enhances the biofilm formation of Bacillus velezensis SQR9 in an McpA-dependent manner, Applied Microbiology and Biotechnology, 2020, 104, 785-797.
45. Chen, D.W.; Yun, J.L.; Qiao, Y.X.; Hu, R.; Hu, B.Y.; Wang, J.; Du, W.B., Chapter 7: Microfluidic techniques for high-throughput cell analysis, in Biomimetic Microengineering, edited by H. J. Kim, CRC Taylor & Francis, 2020, pp.165-189.
46. Wei, W.P.#; Meng, C.#; Wang, Y.H.; Huang, Y.S.; Du, W.B.; Li, H.F.; Liu, Y.F.; Song, H.; Tang, F.S.*, The interaction between self-assembling peptides and emodin and the controlled release of emodin from in-situ hydrogel, Artificial Cells, Nanomedicine, and Biotechnology, 2019, 47, 3961-3975.
47. Zhukov, D.V.; Khorosheva, E.M.; Khazaei, T.; Du, W.B.; Selck, D.A.; Shishkin, A.; Ismagilov, R.F.*, Microfluidic SlipChip device for multistep multiplexed biochemistry on a nanoliter scale, Lab on a Chip, 2019, 47, 3961-3975.
48. Lyu, W.Y.; Yu, M.C.; Qu, H.J.; Yu, Z.Q.; Du, W.B.; Shen, F.*, Slip-driven microfluidic devices for nucleic acid analysis, Biomicrofluidics, 2019, 13, 041502.
49. Yi, Q.L.#; Cai, D.Y.#; Xiao, M.; Nie, M.Y.; Cui, Q.N.; Cheng, J.W.; Li, C.M.; Feng, J.; Urban, G.; Xu, Y.-C.*; Lan, Y.*; Du, W.B.*, Direct Antimicrobial Susceptibility Testing of Bloodstream Infection on SlipChip, Biosensors & Bioelectronics, 2019, 135, 200-207.
50. Nie, M.Y.; Zheng, M.; Li, C.M.; Shen, F.; Liu, M.H.; Luo, H.B.; Song, X.H.; Lan, Y.*; Pan, J.Z.*; Du, W.B.*, Assembled Step Emulsification Device for Multiplex Droplet Digital PCR, Analytical Chemistry, 2019, 91, 1779–1784.
51. Chen, D.W.; Liu, S.-J.; Du, W.B.*, Chemotactic screening of imidazolinone-degrading bacteria by microfluidic SlipChip, Journal of Hazardous Materials, 2019, 366, 512-519.
52. Feng, H.C.; Zhang, N.; Fu, R.X.; Liu, Y.P.; Krell, T.; Du, W.B.; Shao, J.H.; Shen, Q.R.; Zhang, R.F.*, Recognition of dominant attractants by key chemoreceptors mediates recruitment of plant growth-promoting rhizobacteria, Environmental Microbiology, 2019, 21, 402-415.
53. Ruan, C.J.; Zheng, X.-W.; Wang, J.; Song, L.; Zhu, Y.-X.; Du, W.B.; Lu, Z.-J.; Huang, Y.; Huang, L.*; Dai, X.*, Hyphobacterium indicum sp. nov., isolated from deep seawater, and emended description of the genus Hyphobacterium, International Journal of Systematic and Evolutionary Microbiology, 2018, 68(12), 3760-3765.
54. Zhai, Z.M.; Nie, M.Y.; Guan, Y.; Zhang, F.Q.; Chen, L.; Muhammad; Du, W.B. Liu, G.; Tian, Y.C.; Huang, Q.*, A microfluidic surface-enhanced Raman spectroscopy approach for assessing the particle number effect of AgNPs on cytotoxicity, Ecotoxicology and Environmental Safety, 2018, 162, 529-535.
55. Liao, S.L.; Tao, Y.; Du, W.B.*; Wang, Y.P.*, Interfacial emulsification: an emerging monodisperse droplet generation method for microreactors and bioanalysis, Langmuir, 2018, 34, 11655-11666. (Invited Feature Article).
56. 何渊慧; 奕巧莲; 杜文斌; 郑波*, 抗菌药物敏感性快速检测方法研究进展, 中国感染控制杂志, 2018, 17, 455-460.
57. Zhou, N.; Sun, Y.T.; Chen, D.W.; Du, W.B.; Yang, H.; Liu, S.J.*, Harnessing microfluidic streak plate technique to investigate the gut microbiome of Reticulitermes chinensis, MicrobiologyOpen, 2018, e654.
58. Feng H.C.; Zhang, N.; Du, W.B.; Zhang, H.H.; Liu, Y.P.; Fu, R.X.; Shao, J.H.; Zhang, G.S.; Shen, Q.R.; Zhang, R.F.*, Identification of chemotaxis compounds in root exudates and their sensing chemoreceptors in plant growth-promoting rhizobacteria Bacillus amyloliquefaciens SQR9, Molecular Plant-Microbe Interactions, 2018, 31, 995-1005.
59. Xu, B.X.; Hu, B.Y.; Wang, J.; Lan, Y.; Zhu, Y.X.; Dai, X.; Huang, L.; Huang, Y.*; Du, W.B.*, Virgibacillus indicus sp. nov. and Virgibacillus profundi sp. nov, two moderately halophilic bacteria isolated from marine sediment by using the microfluidic streak plates, International Journal of Systematic and Evolutionary Microbiology, 2018, 68, 2015-2023.
60. Jin, Z.J.; Nie, M.Y.; Zhao, T.H.; Xu, J.Y.; Chen, D.W.; Yun, J.L.; Hu, R.; Ma, L.Z.; Du, W.B.*, Dynamic Sessile-droplet Habitats for Controllable Cultivation of Bacterial Biofilm, Small, 2018, 14, 1800658.
61. Wang, S.Y.; Wang, X.Y.; Wang, L.; Pu, Q.S.; Du, W.B.; Guo, G.S.*, Plasma-assisted alignment in the fabrication of microchannel-array-based in-tube solid phase microextraction microchips packed with TiO2 nanoparticles for phosphopeptide analysis, Analytica Chimica Acta, 2018, 1018, 70-77.
62. Cai, D.Y.; Yi, Q.L.; Shen, C.H.; Lan, Y.; Urban, G.; Du, W.B.*, Direct enrichment of pathogens from physiological samples of high conductivity and viscosity using H-filter and positive dielectrophoresis, Biomicrofluidics, 2018, 12, 014109.
63. Qiao, Y.X.; Zhao, X.Y.; Zhu, J.; Tu, R.; Dong, L.B.; Wang, L.; Dong, Z.Y.; Wang, Q.H.; Du, W.B.*, Fluorescence-Activated Droplet Sorting of Lipolytic Microorganisms Using a Compact Optical System, Lab On a Chip, 2018, 18, 190-196.
64. Liao, S.L; Tao, X.L.; Ju, Y.J.; Feng, J.*; Du, W.B.*; Wang, Y.P.*, Multi-Channel Dynamic Interfacial Printing: An Alternative Multi-Component Droplet Generation Technique for Lab in a Drop, ACS Applied Materials and Interfaces, 2017, 9, 4345-4352.
65. 贾仲君*, 蔡元锋, 贠娟莉, 杜文斌, 单细胞、显微计数和高通量测序典型水稻土微生物组的技术比较, 微生物学报, 2017, 57(6), 899-919.
66. Hu, Y.#; Xu, P.#; Luo, J.; He, H.X.*; Du, W.B.*, Absolute Quantification of H5-subtype Avian Influenza Viruses Using Droplet Digital Loop-mediated Isothermal Amplification, Analytical Chemistry, 2017, 89(1), 745-750.
67. Dong, L.B.; Chen, D.W.; Liu, S.J.; Du, W.B.*, Automated Chemotactic Sorting and Single-cell Cultivation of Microbes using Droplet Microfluidics, Scientific Reports, 2016, 6, 24192.
68. Liao, S.L.; He, Y.L.; Wang, D.G.; Dong, L.B.; Du, W.B.*; Wang, Y.P.*, Dynamic Interfacial Printing for Monodisperse Droplets and Polymeric Microparticles, Advanced Materials Technologies, 2016, 1, 1600021.
69. Xu, P.; Zheng, X.; Tao, Y.; Du, W.B.*, Cross-Interface Emulsification for Generating Size-Tuneable Droplets, Analytical Chemistry, 2016, 88, 3171-3177.
70. Jiang, C.Y.#; Dong, L.B.#; Zhao, J.K.#; Hu, X.F.; Shen, C.H.; Qiao, Y.X.; Zhang, X.Y.; Wang, Y.P.; Ismagilov, R.F.; Liu, S.J.*; Du, W.B.*, High throughput Single-cell Cultivation on Microfluidic Streak Plates, Applied Environmental Microbiology, 2016, 82, 2210-2218. (Spotlight).
71. Yu, S.; Su, T.T.; Wu, H.J.; Liu, S.H.; Wang, D.; Zhao, T.H.; Jin, Z.J.; Du, W.B.; Zhu, M.J.; Chua, S.L.; Yang, L.; Zhu, D.Y.; Gu, L.C.*; Ma, L.Z.*, PslG, a self-produced glycosyl hydrolase, triggers biofilm disassembly by disrupting exopolysaccharide matrix, Cell Research, 2015, 25, 1352-1367.
72. Jia, H.Y.*; He, Y.L.; Zhang, X.Y.; Du, W.B.; Wang, Y.P.*, Integrating Ultra-Thermal-Sensitive Fluids into Elastomers for Multifunctional Flexible Sensors, Advanced Electronic Materials, 2015, 1, 1500029.
73. Huang, X.P.; Yang, Y.D.; Shi, J.Z.; Ngo, H.T.; Shen, C.H.; Du, W.B.; Wang, Y.P.*, High-Internal-Phase Emulsion Tailoring Polymer Amphiphilicity towards an Efficient NIR-Sensitive Bacteria Filter, Small, 2015, 11, 4876-4883.
74. Wu, Z.Q.; Du, W.B.; Li, J.Y.; Xia, X.H.*; Fang, Q.*, Establishment of a finite element model for extracting chemical reaction kinetics in a micro-flow injection system with high throughput sampling, Talanta, 2015, 140, 176-182.
75. Liu, X.; Yi, Q.L.; Han, Y.Z.; Liang, Z.N.; Shen, C.H.; Zhou, Z.Y.; Sun, J.L.; Li, Y.Z.; Du, W.B.*; Cao, R.*, A Robust Microfluidic Device for Synthesis and Crystal Growth of Organometallic Polymers with Highly Organized Structures, Angewandte Chemie International Edition, 2015, 54, 1846-1850.
76. Cai, D.Y.; Xiao, M.; Xu, P.; Xu, Y.C.*; Du, W.B.*, An Integrated Microfluidic Device Utilizing Dielectrophoresis and Multiplex Array PCR for Point-of-Care Detection of Pathogens, Lab On a Chip, 2014, 14, 3917-3924. (HOT Article, Front Cover)
77. Shen, C.H.; Xu, P.; Huang, Z.; Liu, S.J.*; Du, W.B.*, Bacterial Chemotaxis on SlipChip, Lab On a Chip, 2014, 14, 3074-3080.
78. Wang, S.J.; Chen, S.M.; Wang, J.N.; Xu, P.; Luo, Y.M.; Nie, Z.X.*; Du, W.B.*, Interface Solution Isoelectric Focusing with in situ MALDI-TOF Mass Spectrometry, Electrophoresis, 2014, 35, 2528-2533.
79. 袁会领; 董立兵; 涂然*; 杜文斌; 贾士儒; 王钦宏*, 液滴微流控芯片系统中微液滴特性表征及氨基酸检测应用, 生物工程学报, 2014, 30, 139-146.
80. Huang, X.P.; Qian, Q.P.; Zhang, X.Y.; Du, W.B.; Xu, H.P.; Wang, Y.P.*, Assembly of Carbon Nanotubes on Polymer Particles: Towards Rapid Shape Change by Near-Infrared Light, Particle & Particle Systems Characterization, 2013, 30, 235-240.
81. Wang, J.; Zhang, X.Y.; Huang, X.P.; Wang, S.J.; Qian, Q.P.; Du, W.B.; Wang, Y.P.*, Forced Assembly of Water-Dispersible Carbon Nanotubes Trapped in Paper for Cheap Gas Sensors, Small, 2013, 9, 3759-3764.
82. Shen, F.; Sun, B.; Kreutz, J.E.; Davydova, E.K.; Du, W.B.; Reddy, P.L.; Joseph, L.J.; Ismagilov, R.F.*, Multiplexed Quantification of Nucleic Acids with Large Dynamic Range Using Multivolume Digital RT-PCR on a Rotational SlipChip Tested with HIV and Hepatitis C Viral Load, Journal of the American Chemical Society, 2011, 133, 17705-17712.
83. Pompano, R.R.; Liu, W.S.; Du, W.B.; Ismagilov, R.F.*, Microfluidics Using Spatially Defined Arrays of Droplets in One, Two, and Three Dimensions, Annual Review of Analytical Chemistry, 2011, 4, 59-81.
84. Kim, H.J.; Du, W.B.; Ismagilov, R.F.* Complex Function by Design Using Spatially Pre-structured Synthetic Microbial Communities: Degradation of Pentachlorophenol in the Presence of Hg(II), Integrative Biology, 2011, 3, 126-133.
85. Shen, F.; Davydova, E.K.; Du, W.B.; Kreutz, J.E.; Piepenburg, O.; Ismagilov, R.F.*, Digital Isothermal Quantification of Nucleic Acids via Simultaneous Chemical Initiation of Recombinase Polymerase Amplification Reactions on SlipChip, Analytical Chemistry, 2011, 83, 3533-3540.
86. Kreutz, J.E.; Munson, T.; Huynh, T.; Shen, F.; Du, W.B.; Ismagilov, R.F.*, Theoretical Design and Analysis of Multivolume Digital Assays with Wide Dynamic Range Validated Experimentally with Microfluidic Digital PCR, Analytical Chemistry, 2011, 83, 8158-8168.
87. Gu, S.Q.; Zhang, Y.X.; Zhu, Y.; Du, W.B.; Yao, B.; Fang, Q.*, Multifunctional Picoliter Droplet Manipulation Platform and Its Application in Single Cell Analysis, Analytical Chemistry, 2011, 83, 7570-7576.
88. Du, W.B.#; Sun, M.#; Gu, S.Q.#; Zhu, Y.; Fang, Q.*, Automated Microfluidic Screening Assay Platform Based on DropLab, Analytical Chemistry, 2010, 82, 9941-9947.
89. Li, L.; Du, W.B.*; Ismagilov, R.F.*, Multiparameter Screening on SlipChip Used for Nanoliter Protein Crystallization Combining Free Interface Diffusion and Microbatch Methods, Journal of the American Chemical Society, 2010, 132, 112-119.
90. Li, L.; Du, W.B.; Ismagilov, R.F.*, User-Loaded SlipChip for Equipment-Free Multiplexed Nanoliter-Scale Experiments, Journal of the American Chemical Society, 2010, 132, 106-111.
91. Kreutz, J.E.; Shukhaev, A.; Du, W.B.; Druskin, S.; Daugulis, O.; Ismagilov, R.F.*, Evolution of Catalysts Directed by Genetic Algorithms in a Plug-Based Microfluidic Device Tested with Oxidation of Methane by Oxygen, Journal of the American Chemical Society, 2010, 132, 3128-3132.
92. Shen, F.; Du, W.B.; Davydova, E.K.; Karymov, M.A.; Pandey, J.; Ismagilov, R.F.*, Nanoliter Multiplex PCR Arrays on a SlipChip, Analytical Chemistry, 2010, 82, 4606-4612.
93. Shen, F.; Du, W.B.; Kreutz, J.E.; Fok, A.; Ismagilov, R.F.*, Digital PCR on a SlipChip, Lab on a Chip, 2010, 10, 2666-2672.
94. Liu, W.S.; Chen, D.L.; Du, W.B.; Nichols, K.P.; Ismagilov, R.F.*, SlipChip for Immunoassays in Nanoliter Volumes, Analytical Chemistry, 2010, 82, 3276-3282.
95. Du, W.B.; Li, L.; Nichols, K.P.; Ismagilov, R.F.*, SlipChip, Lab on a Chip, 2009, 9, 2286-2292.
96. Liu, W.S.; Kim, H.J.; Lucchetta, E.M.; Du, W.B.; Ismagilov, R.F.*, Isolation, Incubation, and Parallel Functional Testing and Identification by FISH of Rare Microbial Single-copy Cells from Multi-species Mixtures Using the Combination of Chemistrode and Stochastic Confinement, Lab on a Chip, 2009, 9, 2153-2162.
97. Zhang, T.; Fang, Q.*; Du, W.B.; Fu, J.L., Microfluidic Picoliter-Scale Translational Spontaneous Sample Introduction for High-Speed Capillary Electrophoresis, Analytical Chemistry, 2009, 81, 3693-3698.
98. Chen, D.L.; Du, W.B.; Ismagilov, R.F.*, Using TIRF Microscopy to Quantify and Confirm Efficient Mass Transfer at the Substrate Surface of the Chemistrode, New Journal of Physics, 2009, 11, 75017.
99. 姚波; 何巧红; 杜文斌; 石晓彤; 方群*, 微流控高通量试样引入技术的研究进展, 色谱, 2009, 27, 662-666.
100. Chen, D.L.#; Du, W.B.#; Liu, Y.#; Liu, W.S.#; Kuznetsov, A.; Mendez, F.E.; Philipson, L.H.; Ismagilov, R.F.*, The chemistrode: A Droplet-based Microfluidic Device for Stimulation and Recording with High Temporal, Spatial, and Chemical Resolution, Proceedings of the National Academy of Sciences of the United States of America, 2008, 105, 16843-16848.
101. Huang, Y.Z.; Du, W.B.; Pan, J.Z.; Fang, Q.*, Microfluidic chip-based valveless flow injection analysis system with gravity-driven flows, Analyst, 2008, 133, 1237-1241.
102. Fang, Q.*; Shi, X.T.; Du, W.B.; He, Q.H.; Shen, H.; Fang, Z.L., High-throughput microfluidic sample-introduction systems, Trends in Analytical Chemistry, 2008, 27, 521-532.
103. Fan, X.F.; Li, Q.; Wang, S.L.; Xu, Z.R.*; Du, W.B.; Fang, Q.; Fang, Z.L., High-throughput analysis of DNA fragments using a miniaturized CE system combined with a slotted-vial array sample introduction system, Electrophoresis, 2008, 29, 4733-4738.
104. 叶晓兰; 杜文斌; 古淑青; 潘建章; 牟颖; 方群*, 小型微流控芯片流式细胞仪的研制, 分析化学, 2008, 36, 1443-1446.
105. He, Q.H.; Fang, Q.*; Du, W.B.; Fang, Z.L., Fabrication of a monolithic sampling probe system for automated and continuous sample introduction in microchip-based CE, Electrophoresis, 2007, 28, 2912-2919.
106. Du, W.B.; Fang, Q.*; Fang, Z.L., Microfluidic Sequential Injection Analysis in a Short Capillary, Analytical Chemistry, 2006, 78, 6404-6410.
107. Sun, M.; Du, W.B.; Fang, Q.*, Microfluidic Liquid-liquid Extraction System based on Stopped-flow Technique and Liquid Core Waveguide Capillary, Talanta, 2006, 70, 392-396.
108. Du, W.B.; Fang, Q.*; He, Q.H.; Fang, Z.L., High-throughput Nanoliter Sample Introduction Microfluidic Chip-based Flow Injection Analysis System with Gravity-driven Flows, Analytical Chemistry, 2005, 77, 1330-1337.
109. He, Q.H.; Fang, Q.*; Du, W.B.; Huang, Y.Z.; Fang, Z.L.*, An automated electrokinetic continuous sample introduction system for microfluidic chip-based capillary electrophoresis, Analyst, 2005, 130, 1052-1058.
110. 刘军; 方群*; 杜文斌, 缺口型试样管阵列进样系统与紫外检测毛细管电泳联用技术, 分析化学, 2005, 33, 1799-1802.
111. 方群*; 杜文斌; 何巧红; 方肇伦, 微流控芯片高通量试样引入系统的研究, 高等学校化学学报, 2004, 25, 1442-1446.
112. 杜文斌; 方群*; 方肇伦, 基于液芯波导原理的微流控芯片长光程光度检测系统, 高等学校化学学报, 2004, 25, 610-613.

microfluidics, droplets, single-cell analysis, cell-cell interaction, microbial diversity, antibiotic resistance, point-of-care testing (POCT), high throughput screening (HTS).

​

已指导学生

奕巧莲  博士研究生  071005-微生物学  

许晶月  硕士研究生  071005-微生物学  

陶艺  博士研究生  071005-微生物学  

聂梦月  硕士研究生  085238-生物工程  

靳增军  博士研究生  071007-遗传学  

徐冰雪  硕士研究生  071005-微生物学  

聂梦月  博士研究生  071005-微生物学  

乔雨歆  博士研究生  071005-微生物学  

胡然  硕士研究生  085238-生物工程  

郑蒙  硕士研究生  085238-生物工程  

谢冰亮  博士研究生  071005-微生物学  

李埰明  硕士研究生  071005-微生物学  

胡倍瑜  博士研究生  071005-微生物学  

武斌杰  博士研究生  071005-微生物学  

于海燕  硕士研究生  071005-微生物学  

汪之一  博士研究生  071005-微生物学  

吴晓琳  硕士研究生  071005-微生物学  

朱晓雅  硕士研究生  086000-生物与医药  

李埰明  博士研究生  071005-微生物学  

现指导学生

郭思思  博士研究生  071005-微生物学  

汤蔓琪  硕士研究生  071005-微生物学  

吴晓琳  博士研究生  071005-微生物学  

汤佳萍  硕士研究生  086000-生物与医药  

薛俊静  博士研究生  071005-微生物学