基本信息

崔丽  女  博导 优青  中国科学院城市环境研究所

电子邮件: lcui@iue.ac.cn
通信地址: 厦门市集美大道1799号
研究兴趣:环境生物单细胞分析方法;环境抗生素抗性和病原菌监测;土壤-植物有益微生物资源
代表作:PNAS, Angew Chem, J Am Chem Soc, Environ. Sci. Technol. (6篇), Anal Chem (10篇)等


研究领域

基于单细胞技术的环境微生物安全和有益资源研究。长期从事环境微生物单细胞拉曼/荧光前沿技术研发,开展原位复杂环境中生物安全监测防控(抗生素耐药性、病原菌)和有益生物资源挖掘(固氮、解磷、塑料降解)研究。

招生信息

单细胞分析,环境微生物

招生专业
083001-环境科学
071005-微生物学
0703Z1-化学生物学
招生方向
环境微生物分析,抗生素抗性/病原体监测防控,塑料降解、土壤-植物有益菌挖掘
抗生素抗性/病原菌监测,土壤-植物有益菌
荧光/拉曼生物探针标记,微流控

教育背景

2007-06--2007-12   英国南安普顿大学   访问学生
2002-09--2008-06   厦门大学化学系   理学博士(物理化学)
1998-09--2002-07   厦门大学化学系   学士
学历
-- 研究生
学位
-- 博士

工作经历

   
工作简历
2019-01~现在, 中国科学院城市环境研究所, 研究员
2015-08~2016-02,英国牛津大学, 访问学者
2015-02~2015-07,英国兰卡斯特大学, 访问学者
2010-10~2018-12,中科院城市环境研究所, 副研究员
2009-11~2010-02,瑞士伯尔尼大学, 访问学者
社会兼职
2023-01-01-今,美国微生物学会期刊mSystems编辑,
2023-01-01-今,环境化学青年编委,
2023-01-01-今,厦门大学兼职教授,
2022-10-01-今,光散射学报编委,
2022-06-01-今,中国科学技术大学兼职博导,

教授课程

基于单细胞拉曼光谱的环境微生物研究

专利与奖励

   
奖励信息
(1) 福建省科学技术二等奖, 二等奖, 省级, 2020
专利成果
( 1 ) 一种细胞培养芯片, 发明专利, 2022, 第 1 作者, 专利号: CN114836321A

( 2 ) 一种基于单细胞拉曼光谱的好氧不产氧光合细菌检测方法, 发明专利, 2022, 第 2 作者, 专利号: CN113984659A

( 3 ) 一种耐药微生物的筛选方法, 发明专利, 2021, 第 1 作者, 专利号: CN111849780B

( 4 ) 一种高灵敏汞离子荧光传感材料及其制备方法和应用以及检测溶液中汞离子浓度的方法, 发明专利, 2021, 第 5 作者, 专利号: CN112920189A

( 5 ) 一种基于拉曼光谱-重水同位素标记的耐药菌药敏性快速检测方法和判断合理用药的方法, 专利授权, 2018, 第 1 作者, 专利号: CN108267436A

( 6 ) 一种基于单细胞拉曼光谱和 15 N 2 稳定同位素标记的固氮菌检测方法, 发明专利, 2018, 第 1 作者, 专利号: CN108267437A

( 7 ) 一种基于单细胞拉曼光谱和 15 N 2 稳定同位素标记的固氮菌检测方法, 发明专利, 2018, 第 1 作者, 专利号: CN108267437A

( 8 ) 表面增强拉曼光谱基底和样品的制备方法, 发明专利, 2017, 第 1 作者, 专利号: CN106770171A

出版信息

   
发表论文
(1) Nitrogen fixation and diazotroph diversity in groundwater systems, ISME JOURNAL, 2023, 第 8 作者
(2) Impact of Airborne Pathogen-Derived Extracellular Vesicles on Macrophages Revealed by Raman Spectroscopy and Multiomics, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2023, 通讯作者
(3) An Isotope-Labeled Single-Cell Raman Spectroscopy Approach for Tracking the Physiological Evolution Trajectory of Bacteria toward Antibiotic Resistance, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2023, 通讯作者
(4) Widespread of Potential Pathogen-Derived Extracellular Vesicles Carrying Antibiotic Resistance Genes in Indoor Dust, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2022, 通讯作者
(5) Viral diversity and potential environmental risk in microplastic at watershed scale: Evidence from metagenomic analysis of plastisphere, ENVIRONMENT INTERNATIONAL, 2022, 第 3 作者
(6) Domestic refrigerators: An overlooked breeding ground of antibiotic resistance genes and pathogens, ENVIRONMENT INTERNATIONAL, 2022, 通讯作者
(7) Deep Learning-Enabled Raman Spectroscopic Identification of Pathogen-Derived Extracellular Vesicles and the Biogenesis Process, ANALYTICAL CHEMISTRY, 2022, 通讯作者
(8) 土壤病原微生物检测技术研究进展, Advances in the detection technology of pathogenic microorganisms in soil, 农业环境科学学报, 2022, 第 5 作者
(9) Urbanization drives the succession of antibiotic resistome and microbiome in a river watershed, CHEMOSPHERE, 2022, 通讯作者
(10) Teng Wen-Kai-Urban Environment and, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2022, 第 2 作者
(11) Effects of soil protists on the antibiotic resistome under long term fertilization, ENVIRONMENTAL POLLUTION, 2022, 第 6 作者
(12) Effects of soil protists on the antibiotic resistome under long term fertilization, ENVIRONMENTAL POLLUTION, 2022, 第 6 作者
(13) Temporal variation of antibiotic resistome and pathogens in food waste during short-term storage, Journal of Hazardous Materials, 2022, 通讯作者
(14) Active antibiotic resistome in soils unraveled by single-cell isotope probing and targeted metagenomics, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2022, 通讯作者
(15) Macleaya cordata extract, an antibiotic alternative, does not contribute to antibiotic resistance gene dissemination, JOURNAL OF HAZARDOUS MATERIALS, 2021, 第 6 作者
(16) Raman biosensor and molecular tools for integrated monitoring of pathogens and antimicrobial resistance in wastewater, TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2021, 通讯作者
(17) Long-Term Fertilization History Alters Effects of Microplastics on Soil Properties, Microbial Communities, and Functions in Diverse Farmland Ecosystem, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2021, 第 6 作者
(18) Impact of Urbanization on Antibiotic Resistome in Different Microplastics: Evidence from a Large-Scale Whole River Analysis, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2021, 通讯作者
(19) Potential of indigenous crop microbiomes for sustainable agriculture, NATURE FOOD, 2021, 第 4 作者
(20) High starter phosphorus fertilization facilitates soil phosphorus turnover by promoting microbial functional interaction in an arable soil, High starter phosphorus fertilization facilitates soil phosphorus turnover by promoting microbial functional interaction in an arable soil, JOURNAL OF ENVIRONMENTAL SCIENCES, 2020, 第 6 作者
(21) 单细胞稳定同位素标记技术在固氮微生物中的应用研究, Application of single-cell stable isotope probing approach to investigate N2-fixing microorganisms, 微生物学报, 2020, 第 2 作者
(22) Temporal Dynamics of Antibiotic Resistome in the Plastisphere during Microbial Colonization, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2020, 通讯作者
(23) Phenotypic Tracking of Antibiotic Resistance Spread via Transformation from Environment to Clinic by Reverse D2O Single-Cell Raman Probing, ANALYTICAL CHEMISTRY, 2020, 通讯作者
(24) Population and single cell metabolic activity of UV-induced VBNC bacteria determined by CTC-FCM and D2O-labeled Raman spectroscopy, ENVIRONMENTINTERNATIONAL, 2019, 第 3 作者
(25) Fate of Labile Organic Carbon in Paddy Soil Is Regulated by Microbial Ferric Iron Reduction, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2019, 第 4 作者
(26) Perspective on Surface-Enhanced Raman Spectroscopic Investigation of Microbial World, ANALYTICAL CHEMISTRY, 2019, 通讯作者
(27) Loss of soil microbial diversity exacerbates spread of antibiotic resistance, Loss of soil microbial diversity exacerbates spread of antibiotic resistance, 土壤生态学快报, 2019, 第 6 作者
(28) 基于拉曼光谱的快速细菌诊断, Rapid Bacterial Diagnosis by Raman Spectroscopy, 光散射学报, 2019, 第 4 作者
(29) Metabolic Inactivity and Re-awakening of a Nitrate Reduction Dependent Iron(II)-Oxidizing Bacterium Bacillus ferrooxidans, FRONTIERS IN MICROBIOLOGY, 2019, 第 5 作者
(30) D2O-Isotope-Labeling Approach to Probing Phosphate-Solubilizing Bacteria in Complex Soil Communities by Single-Cell Raman Spectroscopy, ANALYTICAL CHEMISTRY, 2019, 通讯作者
(31) Heavy water-labeled Raman spectroscopy reveals carboxymethylcellulose-degrading bacteria and degradation activity at the single-cell level, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2019, 通讯作者
(32) Standardization of complex biologically derived spectrochemical datasets, NATURE PROTOCOLS, 2019, 第 3 作者
(33) Does nano silver promote the selection of antibiotic resistance genes in soil and plant?, ENVIRONMENT INTERNATIONAL, 2019, 通讯作者
(34) Rapid Antibiotic Susceptibility Testing of Pathogenic Bacteria Using Heavy-Water-Labeled Single-Cell Raman Spectroscopy in Clinical Samples, ANALYTICAL CHEMISTRY, 2019, 通讯作者
(35) Tracking antibiotic resistome during wastewater treatment using high throughput quantitative PCR, ENVIRONMENT INTERNATIONAL, 2018, 第 7 作者
(36) Response to Comment on "Application of Struvite Alters the Antibiotic Resistome in Soil, Rhizosphere, and Phyllosphere", ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2018, 通讯作者
(37) Functional single-cell approach to probing nitrogen-fixing bacteria in soil communities by resonance raman spectroscopy with n-15(2) labeling, ANALYTICAL CHEMISTRY, 2018, 第 1 作者
(38) SERS-稳定同位素标记技术研究氮相关功能/活性微生物, Surface-Enhanced Raman Spectroscopy Combined with Stable Isotope Probing to Study Nitrogen Assimilation by Microorganisms, 光散射学报, 2018, 第 1 作者
(39) Functional Single-Cell Approach to Probing Nitrogen-Fixing Bacteria in Soil Communities by Resonance Raman Spectroscopy with (15)N2 Labeling., Anal Chem, 2018, 第 1 作者
(40) Effect of biochar amendment on the alleviation of antibiotic resistance in soil and phyllosphere of Brassica chinensis L., SOIL BIOLOGY & BIOCHEMISTRY, 2018, 通讯作者
(41) Induction of Escherichia coli Into a VBNC State by Continuous-Flow UVC and Subsequent Changes in Metabolic Activity at the Single-Cell Level, FRONTIERS IN MICROBIOLOGY, 2018, 通讯作者
(42) Diet-sourced carbon-based nanoparticles induce lipid alterations in tissues of zebrafish (Danio rerio) with genomic hypermethylation changes in brain, MUTAGENESIS, 2017, 第 6 作者
(43) Surface-Enhanced Raman Spectroscopy Combined with Stable Isotope Probing to Monitor Nitrogen Assimilation at Both Bulk and Single-Cell Level, ANALYTICAL CHEMISTRY, 2017, 通讯作者
(44) Application of genomic technologies to measure and monitor antibiotic resistance in animals, ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 2017, 第 2 作者
(45) Diet-sourced carbon-based nanoparticles induce lipid alterations in tissues of zebrafish (Danio rerio) with genomic hypermethylation changes in brain, MUTAGENESIS, 2017, 第 6 作者
(46) Application of struvite alters the antibiotic resistome in soil, rhizosphere, and phyllosphere, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2017, 通讯作者
(47) Surface-Enhanced Raman Spectroscopy Combined with Stable Isotope Probing to Monitor Nitrogen Assimilation at Both Bulk and Single-Cell Level, ANALYTICAL CHEMISTRY, 2017, 通讯作者
(48) Raman-Deuterium Isotope Probing for in-situ identification of antimicrobial resistant bacteria in Thames River, SCIENTIFIC REPORTS, 2017, 第 2 作者
(49) Do manure-borne or indigenous soil microorganisms influence the spread of antibiotic resistance genes in manured soil?, SOIL BIOLOGY & BIOCHEMISTRY, 2017, 通讯作者
(50) Single cell stable isotope probing in microbiology using Raman microspectroscopy, CURRENT OPINION IN BIOTECHNOLOGY, 2016, 第 3 作者
(51) Biogenic silver nanocomposite TFC nanofiltration membrane with antifouling properties, DESALINATION AND WATER TREATMENT, 2016, 第 4 作者
(52) Low-dose carbon-based nanoparticle-induced effects in A549 lung cells determined by biospectroscopy are associated with increases in genomic methylation, SCIENTIFIC REPORTS, 2016, 第 3 作者
(53) Aluminium foil as a potential substrate for ATR-FTIR, transflection FTIR or Raman spectrochemical analysis of biological specimens, ANALYTICAL METHODS, 2016, 通讯作者
(54) Surface-Enhanced Raman Spectroscopy for Identification of Heavy Metal Arsenic(V)-Mediated Enhancing Effect on Antibiotic Resistance, ANALYTICAL CHEMISTRY, 2016, 第 1 作者
(55) Dosage- and time-dependent antibacterial effect of zinc oxide nanoparticles determined by a highly uniform SERS negating undesired spectral variation, ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2016, 通讯作者
(56) Real-time Raman based approach for identification of biofouling, SENSORS & ACTUATORS: B. CHEMICAL, 2016, 第 3 作者
(57) Single cell stable isotope probing in microbiology using Raman microspectroscopy, CURRENT OPINION IN BIOTECHNOLOGY, 2016, 第 3 作者
(58) Surface-enhanced Raman spectroscopy monitoring the development of dual-species biofouling on membrane surfaces, JOURNAL OF MEMBRANE SCIENCE, 2015, 通讯作者
(59) Effect of toxicity of Ag nanoparticles on SERS spectral variance of bacteria, SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2015, 通讯作者
(60) Interrogating chemical variation via layer-by-layer SERS during biofouling and cleaning of nanofiltration membranes with further investigations into cleaning efficiency, WATER RESEARCH, 2015, 第 1 作者
(61) Interaction of silver nanoparticles with pure nitrifying bacteria, CHEMOSPHERE, 2013, 第 3 作者
(62) Structural and Charge Sensitivity of Surface-Enhanced Raman Spectroscopy of Adenine on Silver Surface: A Quantum Chemical Study, JOURNAL OF PHYSICAL CHEMISTRY C, 2013, 第 3 作者
(63) Effects of Bio-Au Nanoparticles on Electrochemical Activity of Shewanella oneidensis Wild Type and Delta omcA/mtrC Mutant, SCIENTIFIC REPORTS, 2013, 第 2 作者
(64) In Situ Study of the Antibacterial Activity and Mechanism of Action of Silver Nanoparticles by Surface-Enhanced Raman Spectroscopy, ANALYTICAL CHEMISTRY, 2013, 通讯作者
(65) 表面增强拉曼光谱研究多糖和蛋白对PVDF膜的膜污染能力(英文), Surface-enhanced Raman Spectroscopy Study of the Fouling Propensity of Polysaccharide and Proteins on PVDF Membrane, 光散射学报, 2012, 第 1 作者
(66) 表面增强拉曼光谱研究多糖和蛋白对PVDF膜的膜污染能力, 光散射学报, 2012, 第 1 作者
(67) The ratio of food-to-microorganism (F/M) on membrane fouling of anaerobic membrane bioreactors treating low-strength wastewater, DESALINATION, 2012, 第 3 作者
(68) 表面增强拉曼光谱研究多糖和蛋白对PVDF膜的膜污染能, 光散射学报, 2012, 第 1 作者
(69) In Situ Gap-Mode Raman Spectroscopy on Single-Crystal Au(100) Electrodes: Tuning the Torsion Angle of 4,4 '-Biphenyldithiols by an Electrochemical Gate Field, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2011, 第 1 作者
(70) Sensitive and Versatile Detection of the Fouling Process and Fouling Propensity of Proteins on Polyvinylidene Fluoride Membranes via Surface-Enhanced Raman Spectroscopy, ANALYTICAL CHEMISTRY, 2011, 第 1 作者
(71) Distributions of typical contaminant species in urban short-term storm runoff and their fates during rain events: A case of Xiamen City, JOURNALOFENVIRONMENTALSCIENCES, 2010, 第 4 作者
(72) 蛋白-多糖比例对膜污染的影响, Characterization of protein–polysaccharide ratios on membrane fouling, DESALINATION, 2010, 第 3 作者
(73) 蛋白-多糖比例对膜污染的影响, Characterization of protein–polysaccharide ratios on membrane fouling, DESALINATION, 2010, 第 3 作者
(74) Surface-Enhanced Raman Spectroscopic Study on the Co-adsorption of DNA Bases with Perchlorate, ACTA PHYSICO-CHIMICA SINICA, 2010, 通讯作者
(75) DNA碱基与高氯酸根共吸附行为的表面增强拉曼光谱研究, Surface-Enhanced Raman Spectroscopic Study on the Co-adsorption of DNA Bases with Perchlorate, 物理化学学报, 2010, 第 1 作者
(76) Charge-Transfer Enhancement Involved in the SERS of Adenine on Rh and Pd Demonstrated by Ultraviolet to Visible Laser Excitation, JOURNAL OF PHYSICAL CHEMISTRY C, 2010, 
(77) 腺嘌呤/钯体系的电荷转移增强机理研究, Charge Transfer Enhancement of Adenine Adsorbed on Palladium Nanostructured Surfaces by UV and Visible Laser Excited SERS, 光散射学报, 2009, 第 1 作者
(78) UV SERS at well ordered Pd sphere segment void (SSV) nanostuctures, Phys Chem Chem Phys., 2009, 第 1 作者
(79) Shaping and Shelling Pt and Pd Nanoparticles for Ultraviolet Laser Excited Surface-Enhanced Raman Scattering, JOURNAL OF PHYSICAL CHEMISTRY C, 2008, 第 1 作者
(80) Orientation change of adsorbed pyrazine on roughened rhodium electrodes as probed by surface-enhanced Raman spectroscopy, JOURNAL OF PHYSICAL CHEMISTRY B, 2005, 
(81) Interaction of silver nanoparticles with pure nitrifying bacteria, 

科研活动

   
科研项目
( 1 ) 膜生物反应器膜污染的表面增强拉曼光谱原位检测方法建立和形成机制研究, 负责人, 国家任务, 2010-01--2012-12
( 2 ) 表面增强拉曼光谱原位研究滤膜表面生物膜的形成机制及影响因素, 负责人, 国家任务, 2012-01--2015-12
( 3 ) 基于表面增强拉曼光谱的碳纳米材料对生物膜毒性机制的原位研究, 负责人, 地方任务, 2015-08--2018-04
( 4 ) 基于表面增强拉曼光谱的碳纳米材料抗菌机制研究, 负责人, 地方任务, 2014-08--2016-08
( 5 ) 中科院青年创新促进会会员基金, 负责人, 中国科学院计划, 2014-01--2017-12
( 6 ) 肥料磷素转化与高效利用机理, 负责人, 国家任务, 2017-01--2020-12
( 7 ) 基于单细胞拉曼-重水标记的环境抗生素抗性菌识别和抗性产生过程研究, 负责人, 国家任务, 2018-01--2021-12
( 8 ) 农田抗生素污染及抗性基因增殖扩散机制, 参与, 国家任务, 2016-01--2020-12
( 9 ) 基于单细胞拉曼-15N2稳定同位素标记解析水圈固氮微生物及其固氮活性, 负责人, 国家任务, 2019-01--2021-12
( 10 ) 基于单细胞的抗生素耐药性研究, 负责人, 中国科学院计划, 2019-09--2023-12
( 11 ) 城市-城郊土壤生物地球化学过程与环境质量, 参与, 国家任务, 2021-07--2025-12
( 12 ) 基于拉曼光谱的环境生物分析, 负责人, 国家任务, 2020-01--2022-12
( 13 ) 基于合成菌群的环境微生物强化靶向消减与控制原理, 负责人, 国家任务, 2023-01--2026-12
( 14 ) 基于单细胞拉曼研究抗生素耐药性从环境菌向人类病原菌的传播机制和风险, 负责人, 国家任务, 2022-01--2025-12
( 15 ) 高通量拉曼流式细胞分选仪, 参与, 国家任务, 2023-05--2027-07
参与会议
(1)Surface-enhanced Raman spectroscopy (SERS) seeing the development of dual-species biofilm on a mixed cellulose membrane   Li. Cui, Peng Yu. Chen, Kai Song. Zhang   2014-08-15
(2)urface-enhanced Raman Spectroscopic Studies of Membrane Organic Fouling and Biofouling   Li Cui, Pengyu Chen, Kaisong Zhang   2014-07-20

指导学生

已指导学生

陈鹏宇  02  63224  

刘莎莎  02  63224  

张碧峰  02  19368  

现指导学生

李弘哲  02  63224