基本信息
陈义华  男  博导  中国科学院微生物研究所
电子邮件: chenyihua@im.ac.cn
通信地址: 北京市朝阳区北辰西路1号院3号
邮政编码: 100101

研究领域

微生物次级代谢与人体健康

微生物次级代谢产物是抗肿瘤药物、抗生素、免疫抑制剂等的重要来源之一。微生物次级代谢在多种疾病发生中发挥了重要作用。关于微生物次级代谢的研究对人类生活和健康状况的改善起了极大的推动作用。本实验室致力于运用分子遗传学、生物化学、化学和生物信息学的综合手段来研究微生物生产的具有实用价值的生物活性分子,筛选药物先导化合物,创新健康促进和疾病诊疗的先进手段。主要包括以下三个方面的研究内容:
(1) 挖掘人体致病菌、益生菌、食品等重要微生物中的次级代谢潜力,获取具有特定活性的化合物,解析其作用机制和生理功能,创新健康促进和疾病诊疗的先进手段。

(2)在对微生物药物分子和高活性化合物的生物合成机理充分了解的基础上,设计合理的策略,通过合成生物学和合成衍生等手段改造药物分子,创制新的先导药物。
(3) 利用次级代谢的多样性探索生命最基本的代谢和进化问题,理解生命形式的多样性。

招生信息

   
招生专业
100705-微生物与生化药学
071007-遗传学
071010-生物化学与分子生物学
招生方向
微生物次级代谢
微生物药物的生物合成
天然产物的合成生物学

教育背景

2001-09--2005-07   中国科学院微生物研究所   博士
1998-09--2001-06   南京农业大学   硕士
1994-09--1998-06   南京农业大学   本科

工作经历

   
工作简历
2011-04~现在, 中国科学院微生物研究所, 研究员
2006-07~2011-04,University of Wisconsin-Madison, Postdoc Research Associate

教授课程

天然产物药物的生物合成及生物催化
微生物代谢工程
微生物学-II

专利与奖励

   

出版信息

   
发表论文
[1] Li, Chao, Bao, Wuyundalai, Zhang, Haoyue, Lyu, Zhitang, Chen, Yihua, Guo, Zhengyan. Discovery of Brominated Alboflavusins With Anti-MRSA Activities. FRONTIERS IN MICROBIOLOGY[J]. 2021, 12: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920986/.
[2] Tang, Yue, Tang, Wei, Wang, Min, Zhang, Zhilong, Chen, Yihua. A conservative distribution of tridomain NDP-heptose synthetases in actinobacteria. SCIENCE CHINA-LIFE SCIENCES. 2021, 1-10, http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000705169000001.
[3] Guo, Zhengyan, Tang, Yue, Tang, Wei, Chen, Yihua. Heptose-containing bacterial natural products: structures, bioactivities, and biosyntheses. NATURAL PRODUCT REPORTSnull. 2021, 38(10): 1887-1909, [4] Ding, Yong, Wang, Min, Li, Jine, Li, Pengwei, Guo, Zhenyan, Chen, Yihua. Characterization of a 3-hydroxyanthranilic acid 6-hydroxylase involved in paulomycin biosynthesis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2021, 543: 8-14, http://dx.doi.org/10.1016/j.bbrc.2021.01.042.
[5] Pengwei Li, Meng Chen, Wei Tang, Zhengyan Guo, Yuwei Zhang, Min Wang, Geoff P Horsman, Jin Zhong, Zhaoxin Lu, Yihua Chen. Initiating polyketide biosynthesis by on-line methyl esterification. NATURE COMMUNICATIONS[J]. 2021, 12(1): http://dx.doi.org/10.1038/s41467-021-24846-7.
[6] Ding, Yong, Li, Xinli, Horsman, Geoff P, Li, Pengwei, Wang, Min, Li, Jine, Zhang, Zhilong, Liu, Weifeng, Wu, Bian, Tao, Yong, Chen, Yihua. Construction of an Alternative NAD(+) De Novo Biosynthesis Pathway. ADVANCED SCIENCE[J]. 2021, 8(9): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097395/.
[7] Xueyan Yao, Zhenying Zhang, Jun Huang, Shiping Wei, Xianyun Sun, Yihua Chen, Hongwei Liu, Shaojie Li, Robert M Kelly. Candicidin Isomer Production Is Essential for Biocontrol of Cucumber Rhizoctonia Rot by Streptomyces albidoflavus W68. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2021, 87(9): http://dx.doi.org/10.1128/AEM.03078-20.
[8] 陈义华. Deciphering the Biosynthesis of TDP-β‑L‑oleandrose in Avermectin. Journal of Natural Products. 2020, [9] Tang, Wei, Li, Pengwei, Chen, Meng, Guo, Zhengyan, Chen, Yihua. Characterization of SepE and SepF for the N-6-Glycosylated Adenine Structure Formation in Septacidin Biosynthesis. ORGANIC LETTERS[J]. 2020, 22(13): 5251-5254, http://dx.doi.org/10.1021/acs.orglett.0c01918.
[10] 陈义华. Activation of paulomycin production by exogenous γ-butyrolactone signaling molecules in Streptomyces albidoflavus J1074.. Applied Microbiology and Biotechnology. 2020, [11] Wang, Min, Xie, Zhoujie, Tang, Shoubin, Chang, Ee Ling, Tang, Yue, Guo, Zhengyan, Cui, Yinglu, Wu, Bian, Ye, Tao, Chen, Yihua. Reductase of Mutanobactin Synthetase Triggers Sequential C-C Macrocyclization, C-S Bond Formation, and C-C Bond Cleavage. ORGANIC LETTERS[J]. 2020, 22(3): 960-964, https://www.webofscience.com/wos/woscc/full-record/WOS:000513082700040.
[12] Wang, Junyang, Wang, Weishan, Wang, Huizhuan, Yuan, Fang, Xu, Zhen, Yang, Keqian, Li, Zilong, Chen, Yihua, Fan, Keqiang. Improvement of stress tolerance and riboflavin production of Bacillus subtilis by introduction of heat shock proteins from thermophilic bacillus strains. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2019, 103(11): 4455-4465, https://www.webofscience.com/wos/woscc/full-record/WOS:000467209500016.
[13] Tingting Hao, Zhoujie Xie, Min Wang, Liwei Liu, Yuwei Zhang, Weicang Wang, Zhao Zhang, Xuejin Zhao, Pengwei Li, Zhengyan Guo, Shushan Gao, Chunbo Lou, Guodong Zhang, Justin Merritt, Geoff P Horsman, Yihua Chen. An anaerobic bacterium host system for heterologous expression of natural product biosynthetic gene clusters. Nature Communications[J]. 2019, 10(1): 1-13, http://dx.doi.org/10.1038/s41467-019-11673-0.
[14] 陈萌, 唐伟, 郭正彦, 陈义华. L-六碳糖形成机制的研究进展. 微生物学通报[J]. 2019, 46(7): 1678-1694, [15] Urs, Ankanahalli N Nanjaraj, Hu, Yiling, Li, Pengwei, Yuchi, Zhiguang, Chen, Yihua, Zhang, Yan. Cloning and Expression of a Nonribosomal Peptide Synthetase to Generate Blue Rose. ACS SYNTHETIC BIOLOGY[J]. 2019, 8(8): 1698-1704, [16] Tang, Wei, Guo, Zhengyan, Cao, Zhenju, Wang, Min, Li, Pengwei, Meng, Xiangxi, Zhao, Xuejin, Xie, Zhoujie, Wang, Wenzhao, Zhou, Aihua, Lou, Chunbo, Chen, Yihua. D-Sedoheptulose-7-phosphate is a common precursor for the heptoses of septacidin and hygromycin B. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2018, 115(11): 2818-2823, https://www.webofscience.com/wos/woscc/full-record/WOS:000427245400075.
[17] Li, Pengwei, Guo, Zhengyan, Tang, Wei, Chen, Yihua. Activation of three natural product biosynthetic gene clusters from Streptomyces lavendulae CGMCC 4.1386 by a reporter-guided strategy. SYNTHETIC AND SYSTEMS BIOTECHNOLOGY[J]. 2018, 3(4): 254-260, http://lib.cqvip.com/Qikan/Article/Detail?id=7103845359.
[18] 向华. Guo Z, Li P, Chen G, Li C, Cao Z, Zhang Y, Ren J, Xiang H, Lin S, Ju J, Chen Y*. Design and biosynthesis of dimeric alboflavusins with biaryl linkages via regiospecific C-C bond coupling.. Journal of the American Chemical Society, 140(51):18009-18015.. 2018, [19] 陈义华. 通过保罗酸的加成提高保罗霉素的稳定性. 有机化学. 2018, [20] Gao, Chunzhi, Guo, Zhengyan, Lu, Xingzhong, Chen, Haiyan, Liu, Liwei, Yu, Zhiguo, Chen, Yihua. Hexaricins, Pradimicin-like Polyketides from a Marine Sediment-Derived Streptosporangium sp and Their Antioxidant Effects. JOURNAL OF NATURAL PRODUCTS[J]. 2018, 81(9): 2069-2074, https://www.webofscience.com/wos/woscc/full-record/WOS:000446276600019.
[21] 谢周杰, 张昭, 刘力伟, 柳孝晨, 陈义华. 变形链球菌中的次级代谢产物及其在口腔生物被膜中的生态功能. 生物工程学报[J]. 2017, 33(9): 1547-1554, [22] Meng Xiangxi, Wang Wenzhao, Xie Zhoujie, Li Pengwei, Li Yue, Guo Zhengyan, Lu Yingjian, Yang Jie, Guan Kaile, Lu Zhaoxin, Tan Huarong, Chen Yihua. Neomycin biosynthesis is regulated positively by AfsA-g and NeoR in Streptomyces fradiae CGMCC 4.7387. Science China Life Sciences[J]. 2017, [23] Zhang Zhiguo, Tang Yue, Wang Min, Chen Yihua. Synthesis, characterizations and antimicrobial activities of new thiourea derivatives. Microbiology China[J]. 2017, 44(6): 1437-1443, [24] Xie, Zhoujie, Zhang, Zhao, Cao, Zhenju, Chen, Meng, Li, Pengwei, Liu, Weifeng, Qin, Hua, Zhao, Xuejin, Tao, Yong, Chen, Yihua. An external substrate-free blue/white screening system in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2017, 101(9): 3811-3820, https://www.webofscience.com/wos/woscc/full-record/WOS:000399705800025.
[25] Liu, Liwei, Hao, Tingting, Xie, Zhoujie, Horsman, Geoff P, Chen, Yihua. Genome mining unveils widespread natural product biosynthetic capacity in human oral microbe Streptococcus mutans. SCIENTIFIC REPORTS[J]. 2016, 6: [26] Tian, Jun, Chen, Haiyan, Guo, Zhengyan, Liu, Ning, Li, Jine, Huang, Ying, Xiang, Wensheng, Chen, Yihua. Discovery of pentangular polyphenols hexaricins A-C from marine Streptosporangium sp CGMCC 4.7309 by genome mining. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2016, 100(9): 4189-4199, https://www.webofscience.com/wos/woscc/full-record/WOS:000373744000032.
[27] Li, Jine, Wang, Min, Ding, Yong, Tang, Yue, Zhang, Zhiguo, Chen, Yihua. Involvement of an octose ketoreductase and two acyltransferases in the biosynthesis of paulomycins. SCIENTIFIC REPORTS[J]. 2016, 6: https://www.webofscience.com/wos/woscc/full-record/WOS:000370037100001.
[28] Guo, Zhengyan, Li, Jine, Qin, Hua, Wang, Min, Lv, Xun, Li, Xuebing, Chen, Yihua. Biosynthesis of the Carbamoylated D-Gulosamine Moiety of Streptothricins: Involvement of a Guanidino-N-glycosyltransferase and an N-Acetyl-D-gulosamine Deacetylase. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION[J]. 2015, 54(17): 5175-5178, https://www.webofscience.com/wos/woscc/full-record/WOS:000354190800035.
[29] Li, Jine, Xie, Zhoujie, Wang, Min, Ai, Guomin, Chen, Yihua. Identification and Analysis of the Paulomycin Biosynthetic Gene Cluster and Titer Improvement of the Paulomycins in Streptomyces paulus NRRL 8115. PLOS ONE[J]. 2015, 10(3): https://www.webofscience.com/wos/woscc/full-record/WOS:000352134700066.
[30] Li, Pengwei, Li, Jine, Guo, Zhengyan, Tang, Wei, Han, Jianshan, Meng, Xiangxi, Hao, Tingting, Zhu, Yaxin, Zhang, Lixin, Chen, Yihua. An efficient blue-white screening based gene inactivation system for Streptomyces. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2015, 99(4): 1923-1933, https://www.webofscience.com/wos/woscc/full-record/WOS:000350028600032.
[31] Chen, Haiyan, Liu, Ning, Huang, Ying, Chen, Yihua. Isolation of an anthrabenzoxocinone 1.264-C from Streptomyces sp FXJ1.264 and absolute configuration determination of the anthrabenzoxocinones. TETRAHEDRON-ASYMMETRY[J]. 2014, 25(2): 113-116, http://dx.doi.org/10.1016/j.tetasy.2013.11.013.
[32] Li Jine, GUO ZHENGYAN, HUANG WEI, MENG XIANGXI, AI GUOMIN, Tang Gongli, Chen Yihua. Mining of a streptothricin gene cluster from Streptomyces sp TP-A0356 genome via heterologous expression. Sci. China-Life Sci.[J]. 2013, 56(7): 619-627, http://www.irgrid.ac.cn/handle/1471x/870665.
[33] Smanski, Michael J, Yu, Zhiguo, Casper, Jeffrey, Lin, Shuangjun, Peterson, Ryan M, Chen, Yihua, WendtPienkowski, Evelyn, Rajski, Scott R, Shen, Ben. Dedicated ent-kaurene and ent-atiserene synthases for platensimycin and platencin biosynthesis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2011, 108(33): 13498-13503, https://www.webofscience.com/wos/woscc/full-record/WOS:000293895100034.
[34] Chen, Yihua, Yin, Min, Horsman, Geoff P, Shen, Ben. Improvement of the Enediyne Antitumor Antibiotic C-1027 Production by Manipulating Its Biosynthetic Pathway Regulation in Streptomyces globisporus. JOURNAL OF NATURAL PRODUCTS[J]. 2011, 74(3): 420-424, https://www.webofscience.com/wos/woscc/full-record/WOS:000288691800018.
[35] Yin, Min, Lu, Tao, Zhao, LiXing, Chen, Yihua, Huang, ShengXiong, Lohman, Jeremy R, Xu, LiHua, Jiang, ChengLin, Shen, Ben. The Missing C-17 O-Methyltransferase in Geldanamycin Biosynthesis. ORGANIC LETTERS[J]. 2011, 13(14): 3726-3729, https://www.webofscience.com/wos/woscc/full-record/WOS:000292479300045.
[36] 陈义华. Characterization of JadH as an FAD- and NAD(P)H-dependent bifunctional hydroxylase/dehydrase involved in jadomycin biosynthesis.. ChemBioChem.. 2010, [37] Horsman, Geoff P, Chen, Yihua, Thorson, Jon S, Shen, Ben. Polyketide synthase chemistry does not direct biosynthetic divergence between 9-and 10-membered enediynes. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2010, 107(25): 11331-11335, https://www.webofscience.com/wos/woscc/full-record/WOS:000279058000034.
[38] Chen, Yihua, Smanski, Michael J, Shen, Ben. Improvement of secondary metabolite production in Streptomyces by manipulating pathway regulation. APPLIED MICROBIOLOGY AND BIOTECHNOLOGYnull. 2010, 86(1): 19-25, https://www.webofscience.com/wos/woscc/full-record/WOS:000274543200003.
[39] Chen, Yihua, Yin, Min, Horsman, Geoff P, Huang, Shengxiong, Shen, Ben. Manipulation of pathway regulation in Streptomyces globisporus for overproduction of the enediyne antitumor antibiotic C-1027. JOURNAL OF ANTIBIOTICS[J]. 2010, 63(8): 482-485, https://www.webofscience.com/wos/woscc/full-record/WOS:000281499800011.
[40] 陈义华. Characterization of the SgcF epoxide hydrolase supporting a (R)-vicinal diol intermediate for enediyne antitumor antibiotic C-1027 biosynthesis.. Journal of American Chemical Society. 2009, [41] Chen, Yihua, WendtPienkoski, Evelyn, Rajski, Scott R, Shen, Ben. In Vivo Investigation of the Roles of FdmM and FdmM1 in Fredericamycin Biosynthesis Unveiling a New Family of Oxygenases. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2009, 284(37): 24735-24743, http://dx.doi.org/10.1074/jbc.M109.014191.
[42] Chen, Yihua, WendtPienkowski, Evelyn, Shen, Ben. Identification and utility of FdmR1 as a Streptomyces antibiotic regulatory protein activator for fredericamycin production in Streptomyces griseus ATCC 49344 and heterologous hosts. JOURNAL OF BACTERIOLOGY[J]. 2008, 190(16): 5587-5596, https://www.webofscience.com/wos/woscc/full-record/WOS:000258274400006.
[43] Chen, Yihua, Luo, Yinggang, Ju, Jianhua, WendtPienkowski, Evelyn, Rajski, Scott R, Shen, Ben. Identification of fredericamycin E from Streptomyces griseus: Insights into fredericamycin A biosynthesis highlighting carbaspirocycle formation. JOURNAL OF NATURAL PRODUCTS[J]. 2008, 71(3): 431-437, https://www.webofscience.com/wos/woscc/full-record/WOS:000254544900024.
[44] Chen, YH, Wang, CC, Greenwell, L, Rix, U, Hoffmeister, D, Vining, LC, Rohr, JR, Yang, KQ. Functional analyses of oxygenases in jadomycin biosynthesis and identification of JadH as a bifunctional oxygenase/dehydrase. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2005, 280(23): 22508-22514, http://dx.doi.org/10.1074/jbc.M414229200.
[45] Rix, U, Wang, CC, Chen, YH, Lipata, FM, Rix, LLR, Greenwell, LM, Vining, LC, Yang, KQ, Rohr, J. The oxidative ring cleavage in jadomycin biosynthesis: A multistep oxygenation cascade in a biosynthetic black box. CHEMBIOCHEM[J]. 2005, 6(5): 838-+, http://124.16.144.217/handle/311023/1834.
[46] Chen Yihua, Wendt-Pienkowski Evelyn, Ju Jianhua, Lin Shuangjun, Rajski Scott R., Shen Ben. Characterization of FdmV as an Amide Synthetase for Fredericamycin A Biosynthesis in Streptomyces griseus ATCC 43944 *. The Journal of Biological Chemistry. 285(50): 38853-38860, http://dx.doi.org/10.1074/jbc.M110.147744.