生物医学、分析化学

   

070302-分析化学
083100-生物医学工程

微流控芯片；单细胞分析；肿瘤耐药性研究；
体外诊断；生物传感器；
微型核磁共振仪器开发

2008-09--2013-06   华中科技大学   研究生/工学博士
2004-09--2008-06   华中科技大学   本科/工学学士

博士

   

2018-09~现在, 中科院精密测量科学与技术创新研究院, 研究员
2017-07~2018-09,杜克大学（美国）, Research Scientist (助理研究员)
2013-08~2017-06,休斯敦卫理公会医院研究所（美国）, 博士后

2022-01-01-今,Exploration, 青年编委
2021-12-01-今,Cancer Biology&Medicine, 青年编委
2020-08-01-今,《Military Medical Research》, 青年编委
2019-05-06-今,International Research Conference Committee, Member and Reviewer
2017-12-31-今,杂志审稿人, 为Lab Chip, Anal Chem, ACS Applied Material and Interface, Chemical Communications, Talanta等多个杂志审稿

   

（1） 武汉市黄鹤英才, 市地级, 2020
（2） 武汉市朝阳计划, 市地级, 2020
（3） 幼儿病毒/细菌感染快速鉴定试剂盒, 一等奖, 市地级, 2019
（4） 创业武汉星光大道年度总决赛优胜奖, 市地级, 2019
（5） 湖北省BaiRen计划, , 省级, 2019
（6） 中科院BaiRen计划, , 院级, 2018

（ 1 ） Multiplexed volumetric bar chart chip for point of care biomarker and/or analyte quantitation, including competitive control, 发明, 2016, 第 2 作者, 专利号: WO2016154113 A1
（ 2 ） Single cell patterning and coordinated transfer of patterned cells, 发明, 2017, 第 3 作者, 专利号: US20170239657A1
（ 3 ） 一种用于高粘度溶液的快速微混合装置, 发明, 2011, 第 2 作者, 专利号: ZL201110344097.4
（ 4 ） 双水力聚焦微混合装置, 发明, 2012, 第 2 作者, 专利号: ZL201210040918.X
（ 5 ） 一种用于生化反应条件筛选的三维浓度梯度阵列装置, 发明, 2020, 第 1 作者, 专利号: 201810822262.4
（ 6 ） 一种全血过滤装置, 发明, 2019, 第 1 作者, 专利号: 2019101583113
（ 7 ） Devices, systems and methods for high throughput single cell analysis, 发明, 2020, 第 2 作者, 专利号: WO2019079399
（ 8 ） 一种用于现场诊断的酶联免疫吸附检测装置及方法, 发明, 2019, 第 1 作者, 专利号: 201910200897.5
（ 9 ） 一种快速可视化的溶血性大肠杆菌O157：H7检测方法, 发明, 2019, 第 1 作者, 专利号: 201910416351.3

   

[1] Li, Zheyu, Bao, Qingjia, Liu, Chaoyang, Li, Ying, Yang, Yunhuang, Liu, Maili. Recent advances in microfluidics-based bioNMR analysis. LAB ON A CHIPnull. 2023, 23(5): 1213-1225, http://dx.doi.org/10.1039/d2lc00876a.
[2] Hu, Rui, Li, Ying, Yang, Yunhuang, Liu, Maili. Mass spectrometry-based strategies for single-cell metabolomics. MASS SPECTROMETRY REVIEWSnull. 2023, 42(1): 67-94, [3] Zhao, Yin, Chen, Dongjuan, Xu, Zhichen, Li, Tao, Zhu, Jiang, Hu, Rui, Xu, Guoyong, Li, Ying, Yang, Yunhuang, Liu, Maili. Integrating CRISPR-Cas12a into a Microfluidic Dual-Droplet Device Enables Simultaneous Detection of HPV16 and HPV18. ANALYTICAL CHEMISTRY. 2023, http://dx.doi.org/10.1021/acs.analchem.2c05320.
[4] Zhou, Hu, Xu, Zhichen, He, Liang, Wang, Zhijie, Zhang, Tao, Hu, Ting, Huang, Fanwei, Chen, Dongjuan, Li, Ying, Yang, Yunhuang, Huang, Xiaoyuan. Coupling CRISPR/Cas12a and Recombinase Polymerase Amplification on a Stand-Alone Microfluidics Platform for Fast and Parallel Nucleic Acid Detection. ANALYTICAL CHEMISTRY. 2023, http://dx.doi.org/10.1021/acs.analchem.2c04713.
[5] Xu, Zhichen, Chen, Dongjuan, Li, Tao, Yan, Jiayu, Zhu, Jiang, He, Ting, Hu, Rui, Li, Ying, Yang, Yunhuang, Liu, Maili. Microfluidic space coding for multiplexed nucleic acid detection via CRISPR-Cas12a and recombinase polymerase amplification. NATURE COMMUNICATIONS[J]. 2022, 13(1): http://dx.doi.org/10.1038/s41467-022-34086-y.
[6] Zheyu Li, Yue Xiong, Shuangli Li, Jiang Zhu, Rui Hu, Ying Li, Yunhuang Yang, Maili Liu. A fast microfluidic mixer enabling rapid preparation of homogeneous PEG and bicelle media for RDC in NMR analysis. CHEMICAL ENGINEERING JOURNAL[J]. 2022, 431: [7] Li, Tao, Hu, Rui, Xia, Jianbo, Xu, Zhichen, Chen, Dongjuan, Xi, Jinou, Liu, BiFeng, Zhu, Jiang, Li, Ying, Yang, Yunhuang, Liu, Maili. G-triplex: A new type of CRISPR-Cas12a reporter enabling highly sensitive nucleic acid detection. BIOSENSORS & BIOELECTRONICS[J]. 2021, 187: http://dx.doi.org/10.1016/j.bios.2021.113292.
[8] Li Ying. Photolithography-free fabrication of photoresist-mold for rapid prototyping of microfluidic PDMS devices. Chinese Chemical Letters. 2021, [9] Li, Ying, Motschman, Jeffrey D, Kelly, Sean T, Yellen, Benjamin B. Injection Molded Microfluidics for Establishing High-Density Single Cell Arrays in an Open Hydrogel Format. ANALYTICAL CHEMISTRY[J]. 2020, 92(3): 2794-2801, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7295173/.
[10] Li, Ying, Zhang, Pengchao, Li, Tao, Hu, Rui, Zhu, Jiang, He, Ting, Yang, Yunhuang, Liu, Maili. A virtual-droplet system for sensing MMP9 activity of single suspended and adhered cancer cells. SENSORS AND ACTUATORS B-CHEMICAL[J]. 2020, 308: http://dx.doi.org/10.1016/j.snb.2020.127749.
[11] Li, Ying, Li, Tao, Liu, BiFeng, Hu, Rui, Zhu, Jiang, He, Ting, Zhou, Xin, Li, Conggang, Yang, Yunhuang, Liu, Maili. CRISPR-Cas12a trans-cleaves DNA G-quadruplexes. CHEMICAL COMMUNICATIONS[J]. 2020, 56(83): 12526-12529, https://www.webofscience.com/wos/woscc/full-record/WOS:000583607100040.
[12] Liu, Chao, Li, Ying, Liu, BiFeng. Micromixers and their applications in kinetic analysis of biochemical reactions. TALANTA[J]. 2019, 205: http://dx.doi.org/10.1016/j.talanta.2019.120136.
[13] Li, Ying, Xuan, Jie, Hu, Rui, Zhang, Pengchao, Lou, Xiaohua, Yang, Yunhuang. Microfluidic triple-gradient generator for efficient screening of chemical space. TALANTA[J]. 2019, 204: 569-575, http://dx.doi.org/10.1016/j.talanta.2019.06.018.
[14] Rui Hu, Chao Liu, Jie Xuan, Youzhi Xu, Tao Li, BiFeng Liu, Ying Li, Yunhuang Yang. 3D Hydrodynamic Flow Focusing-based Micromixer Enables High-Resolution Imaging for Studying the Early Folding Kinetics of G-Quadruplex. SENSORS & ACTUATORS: B. CHEMICAL. 2019, 293: 312-320, http://dx.doi.org/10.1016/j.snb.2019.05.026.
[15] Liu, Zongbin, Chen, Rui, Li, Ying, Liu, Jianqiao, Wang, Ping, Xia, Xuefeng, Qin, Lidong. Integrated Microfluidic Chip for Efficient Isolation and Deformability Analysis of Circulating Tumor Cells. ADVANCED BIOSYSTEMS[J]. 2018, 2(10): https://www.webofscience.com/wos/woscc/full-record/WOS:000446973500011.
[16] Li Ying. Microfluidics Cell Loading-Dock System: Ordered Cellular Array for Dynamic Lymphocyte-Communication Study. Advanced Biosystems. 2017, [17] Li, Ying, Uddayasankar, Uvaraj, He, Bangshun, Wang, Ping, Qin, Lidong. Fast, Sensitive, and Quantitative Point-of-Care Platform for the Assessment of Drugs of Abuse in Urine, Serum, and Whole Blood. ANALYTICAL CHEMISTRY[J]. 2017, 89(16): 8273-8281, https://www.webofscience.com/wos/woscc/full-record/WOS:000407988600015.
[18] Zhang, Kai, Gao, Min, Chong, Zechen, Li, Ying, Han, Xin, Chen, Rui, Qin, Lidong. Single-cell isolation by a modular single-cell pipette for RNA-sequencing. LAB ON A CHIP[J]. 2016, 16(24): 4742-4748, https://www.webofscience.com/wos/woscc/full-record/WOS:000391429300014.
[19] Liu, Chao, Li, Ying, Li, Yiwei, Chen, Peng, Feng, Xiaojun, Du, Wei, Liu, BiFeng. Rapid three-dimensional microfluidic mixer for high viscosity solutions to unravel earlier folding kinetics of G-quadruplex under molecular crowding conditions. TALANTA[J]. 2016, 149: 237-243, http://dx.doi.org/10.1016/j.talanta.2015.11.036.
[20] Li, Ying, Xuan, Jie, Song, Yujun, Qi, Wenjin, He, Bangshun, Wang, Ping, Qin, Lidong. Nanoporous Glass Integrated in Volumetric Bar-Chart Chip for Point-of-Care Diagnostics of Non-Small Cell Lung Cancer. ACS NANO[J]. 2016, 10(1): 1640-1647, https://www.webofscience.com/wos/woscc/full-record/WOS:000369115800183.
[21] Wang, Yuzhen, Zhu, Guixian, Qi, Wenjin, Li, Ying, Song, Yujun. A versatile quantitation platform based on platinum nanoparticles incorporated volumetric bar-chart chip for highly sensitive assays. BIOSENSORS & BIOELECTRONICS[J]. 2016, 85: 777-784, http://dx.doi.org/10.1016/j.bios.2016.05.090.
[22] Li, Ying, Xuan, Jie, Song, Yujun, Wang, Ping, Qin, Lidong. A microfluidic platform with digital readout and ultra-low detection limit for quantitative point-of-care diagnostics. LAB ON A CHIP[J]. 2015, 15(16): 3300-3306, http://dx.doi.org/10.1039/c5lc00529a.
[23] Li, Ying, Liu, Chao, Feng, Xiaojun, Xu, Youzhi, Liu, BiFeng. Ultrafast Microfluidic Mixer for Tracking the Early Folding Kinetics of Human Telomere G-Quadruplex. ANALYTICAL CHEMISTRY[J]. 2014, 86(9): 4333-4339, https://www.webofscience.com/wos/woscc/full-record/WOS:000335719900039.

（1） Volumetric Bar-Chart Chips for Biosensing, Biomedical Nanotechnology: Methods and Protocols., Springer New York, 2017-07, 第 2 作者

   

（ 1 ） 湖北省BaiRen计划, 主持, 省级, 2020-01--2023-12
（ 2 ） 中科院BaiRen计划, 主持, 部委级, 2018-10--2023-12
（ 3 ） 多通道微流控的超灵敏磁共振仪器系统, 参与, 部委级, 2020-01--2021-12
（ 4 ） 基于微流控芯片的单细胞平台用于白血病细胞异质性和耐药性分析, 主持, 国家级, 2020-01--2022-12
（ 5 ） 基于微流控芯片与NMR技术的类细胞环境下TERRA-G4结构与功能研究, 主持, 国家级, 2022-01--2025-12

（1）基于微流控芯片的高通量单细胞分析平台   第三届微流控技术应用创新论坛（分会场主持及邀请口头报告）    2019-12-07
（2）闭合式和开放式微流控芯片用于高通量单细胞分析   第七届微流控芯片高端论坛会议第三届微流控技术应用创新论坛（分会场主持及邀请口头报告）   2019-11-22
（3）Ultra-Sensitive Point-of-Care Detection of PSA Using an Enzymeand Equipment-Free Microfluidic Platform   第八届国际纳米材料与纳米工程会议第三届微流控技术应用创新论坛（分会场主持及邀请口头报告）   2019-10-11

杜克大学

武汉大学

华中科技大学

现指导学生

覃珊珊  硕士研究生  070302-分析化学  

徐志辰  硕士研究生  070302-分析化学