大脑作为生物体内结构最复杂且功能最强大的器官，拥有约860亿个神经元以及数量相当的胶质细胞，每个神经元能与其它神经元形成多达1万个突触连接，从而构成一个庞大的互连网络。工具对研究复杂的大脑至关重要，如何开发和运用新的工具，从而为解析大脑功能以及脑疾病发生机制带来突破是重大挑战。

我们致力于结合荧光探针技术开发、活体成像、光遗传学/药理学/基因编辑操控和化学生物学手段研究大脑细胞间的通讯机制与功能。具体研究内容包括：

1）核苷类分子的检测及其在生理（睡眠等）及病理（癫痫、中风等）过程的功能；

2）大脑中不同细胞类型的能量代谢规律和调控机制；

3)   结合化学生物学、新型成像工具和操控手段研究神经-肿瘤之间的通讯。

   

071006-神经生物学
071003-生理学
071009-细胞生物学

细胞通讯，突触调控，嘌呤信号
神经递质，探针开发，荧光成像
神经代谢，神经疾病，神经-肿瘤

2013-09--2019-06   北京大学   理学博士
2009-09--2013-06   西北农林科技大学   农学学士

2023-10--现在   中国科学院遗传与发育生物学研究所   研究员
2022-07--2023-09   北大-清华生命科学联合中心   杰出博士后
2019-07--2022-06   勃林格殷格翰公司-北京大学   博士后
2018-08--2018-11   中国科学院脑科学与智能技术卓越创新中心   交流学生

# Equal contributions; * Corresponding author

Representative publications:

8.  Zhou, X.#, He, Y.#, Xu, T.#, Wu, Z.#, Guo, W., Xu, X., ... & Chen, J. F.* (2024). 40 Hz light flickering promotes sleep through cortical adenosine signaling. Cell Research, 1-18.

7.  Wu, Z.#, Cui, Y.#, Wang, H.#, …, Xu, M., Luo, M.* & Li, Y.*(2023), Neuronal activity-induced, ENT-dependent, somatodendritic adenosine release revealed by a GRAB sensor. PNAS, 120(14), e2212387120.

6.  Wu, Z.*, He, K., Chen, Y., Li, H., Pan, S., Li, B., Liu, T., Xi, F., Deng, F., Wang, H., Du, J., Jing, M., & Li, Y.* (2022). A sensitive GRAB sensor for detecting extracellular ATP in vitro and in vivo. Neuron, 110, 770-782 e775.

—Selected as the "Best of Neuron 2021-2022".

5.  Wu, Z., Lin, D. & Li, Y.* (2022). Pushing the frontiers: tools for monitoring neurotransmitters and neuromodulators. Nature Reviews Neuroscience, 23, 257–274.

4.  Qian, C., Wu, Z.#, Sun, R.#, Yu, H., Zeng, J., Rao, Y., & Li, Y.* (2021). Localization, proteomics, and metabolite profiling reveal a putative vesicular transporter for UDP-glucose. eLife, 10, e65417.

3.  Peng, W.#, Wu, Z.#, Kun, S.#, Zhang, S., Li, Y. & Min, X.* (2020). Regulation of sleep homeostasis mediator adenosine by basal forebrain glutamatergic neurons. Science, 369, 1208.

—Highlights by Whalley K., Nature Reviews Neuroscience. 2020 Nov;21(11):593-593.

2.  Wu, Z.*, & Li, Y.* (2020). New frontiers in probing the dynamics of purinergic transmitters in vivo. Neuroscience Research, 35-43. (Cover).

1.  Wu, Z.#, Feng, J.#, Jing, M., & Li, Y.* (2019). G protein-assisted optimization of GPCR-activation based (GRAB) sensors. Neural Imaging and Sensing 2019, vol. 10865, p. 108650N. International Society for Optics and Photonics.

Other publications:

19.  Park, G.#, Lee, G.#, …, Wu, Z., Li, Y., & Choi M.* (2024). Glia-like taste cells mediate an intercellular mode of peripheral sweet adaptation. Cell.

18.  Li, H., Zhao, Y., …, Wu, Z., Li, Y., & Luo, M.* (2024). Astrocytes release ATP/ADP and glutamate in flashes via vesicular exocytosis. Molecular Psychiatry, 1-15.

17.  Ji, E., Zhang, Y., Li, Z., Wei, L., Wu, Z., Li, Y., Yu, X.*, & Song, T. J.* (2024). The Chemokine CCL2 Promotes Excitatory Synaptic Transmission in Hippocampal Neurons via GluA1 Subunit Trafficking. Neuroscience Bulletin, 1-18.

16.  Berki, P.#, Cserép, C.#, Környei, Z.#, …, Wu, Z., ... & Dénes, Á.* (2024). Microglia contribute to neuronal synchrony despite endogenous ATP-related phenotypic transformation in acute mouse brain slices. Nature Communications, 15(1), 5402.

15.  Chen, Y.#, Luan, P.#, Liu, J.#, …, Wu, Z., & Jing, M.* (2024). Spatiotemporally selective astrocytic ATP dynamics encode injury information sensed by microglia following brain injury in mice. Nature Neuroscience, 1-12.

14.  Roy, K.#, Zhou, X.#, Otani, R.#, Yuan, P. C.#, …, Wu, Z., ... & Lazarus, M.* (2024). Optochemical control of slow-wave sleep in the nucleus accumbens of male mice by a photoactivatable allosteric modulator of adenosine A2A receptors. Nature communications, 15(1), 3661.

13.  Umpierre, A. D.#, Li, B.#, …, Wu, Z., ... & Wu, L. J.* (2024). Microglial P2Y6 calcium signaling promotes phagocytosis and shapes neuroimmune responses in epileptogenesis. Neuron.

12.  Bayazitov, I. T., Teubner, B. J., Feng, F., Wu, Z., Li, Y., Blundon, J. A., & Zakharenko, S. S.* (2024). Sound-evoked adenosine release in cooperation with neuromodulatory circuits permits auditory cortical plasticity and perceptual learning. Cell Reports, 43(2).

11.  Hatashita, Y., Wu, Z., Fujita H., Kumamoto T., Livet, J., Li, Y., Tanifuji M., & Inoue, T.* (2023). Spontaneous and multifaceted ATP release from astrocytes at the scale of hundreds of synapses. Glia, 71(9), 2250-2265.

10.  Peng, W.#*, Liu, X.#, Ma, G.#, Wu, Z., … Zhang, S.*, & Min, X.* (2023). Adenosine-independent regulation of the sleep–wake cycle by astrocyte activity. Cell Discovery, 9(1), 16.

9.  Li, X., Li, Y., Zhou, Y., …, Wu, Z., ... & Dai, Q.* (2023). Real-time denoising of fluorescence time-lapse imaging enables high-sensitivity observations of biological dynamics beyond the shot-noise limit. Nature Biotechnology, 41, 282–292.

8.  Pittolo, S., Yokoyama, S., Willoughby, D., ... Wu, Z., … & Poskanzer, K. E.* (2022). Dopamine Activates Astrocytes in Prefrontal Cortex via α1-Adrenergic Receptors. Cell Reports, 40.

7.  Lin, R.#*, Zhou, Y.#, Yan, T.#, Wang, R.#, Li, H., Wu, Z., ... & Luo, M.* (2022). Directed evolution of adeno-associated virus for efficient gene delivery to microglia. Nature Methods, 1-10.

6.  Dai, R., Yu, T., Weng, D., Li, H., Cui, Y., Wu, Z., ... & Luo, M.* (2022). A neuropsin-based optogenetic tool for precise control of Gq signaling. Sci. China Life Sci., 1-14.

5.  Roberts B., Lambert E., Livesey J., Wu, Z., Li, Y. & Cragg S.* (2022). Dopamine release in nucleus accumbens is under tonic inhibition by adenosine A1 receptors regulated by astrocytic ENT1 and dysregulated by ethanol. The Journal of Neuroscience, 42, 1738-1751.

4.  Lu, C.#, Ren, J.#, Mo J., …, Wu, Z., Li, Y., Gao, T., Cao, X.* (2022). Glucocorticoid receptor-dependent astrocytes mediate stress vulnerability. Biological Psychiatry, 92(3), 204-215.

3.  Wang, Q.#, Kong, Y.#, Wu, D. Y., ..., Wu, Z., Li, Y., Yang, J.*, & Gao, T.* (2021). Impaired calcium signaling in astrocytes modulates autism spectrum disorder-like behaviors in mice. Nature communications, 12(1), 1-13.

2.  Feng, J., Zhang, C., Lischinsky, J. E., …, Wu, Z., ... Lin, D., Du, J., & Li, Y.* (2019). A genetically encoded fluorescent sensor for rapid and specific in vivo detection of norepinephrine. Neuron, 102(4), 745-761.

1.  Yu, H., Zhao, T., Liu, S., Wu, Q., Johnson, O., Wu, Z., …, Lei, X., Luo, W.* & Li, Y.* (2019). MRGPRX4 is a bile acid receptor for human cholestatic itch. eLife, 8, e48431.