My research focuses on technologies for the rapid detection and early identification of pathogens, primarily encompassing the following two areas:

Development of Highly Sensitive and Rapid Detection Technologies: Dedicated to developing field-deployable rapid detection methods suitable for complex clinical and environmental samples. The focus is on creating high-sensitivity, rapid nucleic acid and antibody/antigen detection technologies to meet the point-of-care testing needs for emerging and re-emerging infectious pathogens.

Next-Generation Sequencing and Unidentified Pathogen Identification: Utilizing Next-Generation Sequencing (NGS) technology to explore its application in the early discovery and identification of "Disease X" and other unknown pathogens, aiming to construct a cutting-edge pathogen surveillance and warning system.

July 2007 ~ July 2010, Postdoctoral research associate in Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198 USA
Sep 2004 ~ July 2007, Ph.D. in Physical Chemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, P. R. China.
Sep 2001 ~ July 2004, M.S. Degree in Analytical Chemistry, Wuhan University, Wuhan, Hubei 430072, P. R. China
Sep 1997 ~ July 2001, Bachelor of Engineering Degree in Industrial Analysis, China University of Geosciences, Wuhan, Hubei 430074, P. R. China

   

Aug 2011~ Current, Associate Professor in Microbiology in Wuhan Institute of Viorology, Chinese Academy of Sciences
Oct 2010~Aug 2011, Assistant Professor in Microbiology in Wuhan Institute of Viorology, Chinese Academy of Sciences

1. Yun Zhang#, Chunhui Huo#, Jin Xiong#, Dongyan Xiong, Ping He, Zhanlong Wu Z, Genqing Yang, Junhua Li, Hongping Wei H*, Junping Yu*. Whole-Genome Conservation Analysis for the Specific and Accurate Detection of Influenza A and B Viruses and Respiratory Syncytial Virus by Quadruplex RT-qPCR. Journal of Medical Virology. 2025 Apr;97(4):e70327. doi: 10.1002/jmv.70327. PMID: 40159799.
2. Bohan Xu, Dongyan Xiong, Xiaoxu Zhang, Hongping Wei*, Junping Yu*. Development of an ARMS-Quadruplex-qPCR assay for the rapid identification of MPXV and the clades Ia, Ib, IIa and IIb. Journal of Virological Methods. 2025 May;334:115125. doi: 10.1016/j.jviromet.2025.115125. Epub 2025 Feb 11. PMID: 39947350.
3. Yun Zhang#, Yibing Zhang#, Ruihua Lun, Qingshan Fu, Yuqiao Chang, Jiansen Du, Yi Zhang*, Junping Yu*. A novel colorimetric strategy based on canine IgM for ultrasensitive and selective detection of Staphylococcus aureus, LWT - Food Science and Technology 2024 August 1, 205: 116525. doi.org/10.1016/j.lwt.2024.116525.
4. Yun Zhang#, Yibing Zhang#, Wei Hu, Wenhao Zhou, Ying Shang, Ping He, Junhua Li, Hongping Wei*, Junping Yu*. Development of an automated processing platform based on luciferase fused TPP17 to detect specific antibody in clinical serum with high sensitivity and rapidity, International journal of biological macromolecules 2024 Dec;283(Pt 1):137530. doi: 10.1016/j.ijbiomac.2024.137530. Epub 2024 Nov 12. PMID: 39537062.
5. Ping He, Wenhao Zhou, Mengwei Jiang, Junping Yu*, Hongping Wei*. Efficient concentration of viral nucleic acid in wastewater through surfactant releasing and a two-step magnetic bead extraction and purification, Science of the Total Environment 2024 Nov 15;951:175742. doi: 10.1016/j.scitotenv.2024.175742. Epub 2024 Aug 23. PMID: 39182763.
6. Ping He, Wenhao Zhou, Hongping Wei *, Junping Yu*. Fast and Ultrasensitive Detection of Monkeypox by a Pyrococcus furiosus Argonaute System Coupled with a Short Amplification. Viruses. 2024 Feb 29;16(3):382. doi: 10.3390/v16030382. PMID: 38543748; PMCID: PMC10975468.
7. Yibing Zhang#, Yun Zhang#, Wenhao Zhou, Ping He, Xueni Sun, Junhua Li, Hongping Wei*, Junping Yu*. Rapid and sensitive detection of SARS-CoV-2 IgM through luciferase luminescence on an automatic platform. Int J Biol Macromol. 2024 Apr;265(Pt 1):130964. doi: 10.1016/j.ijbiomac.2024.130964. Epub 2024 Mar 17. PMID: 38499123.
8. Yun Zhang#, Ge Tian#, Xueni Sun, Xiaoli Yang, Yi Zhang, Wenqing Tan, Liangwei Duan*, Shunxiang Gao*, Junping Yu*. Ultrasensitive colorimetric detection of Staphylococcus aureus using wheat germ agglutinin and IgY as a dual-recognition strategy. Mikrochim Acta. 2024 Mar 19;191(4):209. doi: 10.1007/s00604-024-06288-2. PMID: 38499840.
9. Dongyan Xiong, Xiaoxu Zhang, Bohan Xu, Mengjuan Shi, Min Chen, Zhuo Dong, Jie Zhong, Rui Gong, Chang Wu, Ji Li, Hongping Wei*, Junping Yu*. PHDtools: A platform for pathogen detection and multi-dimensional genetic signatures decoding to realize pathogen genomics data analyses online. Gene. 2024 May 30;909:148306. doi: 10.1016/j.gene.2024.148306. Epub 2024 Feb 24. PMID: 38408616.
10. Huan Liu, Fei Meng, Raphael Nyaruaba, Ping He, Wei Hong, Mengwei Jiang, Dongqing Liu, Wenhao Zhou, Dan Bai, Junping Yu*, Hongping Wei*. A triton X-100 assisted PMAxx-qPCR assay for rapid assessment of infectious African swine fever virus. Front Microbiol. 2022 Dec 5;13:1062544. doi: 10.3389/fmicb.2022.1062544. PMID: 36545208; PMCID: PMC9760672.
11. Dongyan Xiong, Xiaoxu Zhang, Junping Yu*, Hongping Wei*. Distribution of intra-host variations and mutations in the genomes of SARS-CoV-2 and their implications on detection and therapeutics. MedComm (2020). 2022 Dec 2;3(4):e186. doi: 10.1002/mco2.186. PMID: 36474856; PMCID: PMC9717708
12. Wenhao Zhou, Ping He, Huan Liu, Hongping Wei*, Junping Yu*. A luciferase based automated assay for rapid and sensitive detection of SARS-CoV-2 antibodies. Anal Chim Acta. 2023 Jan 15;1238:340633. doi: 10.1016/j.aca.2022.340633. Epub 2022 Nov 16. PMID: 36464447; PMCID: PMC9672313.
13. Jun Luo*, Min Liu, Peng Wang, Qianyuan Li, Chunhua Luo, Hongping Wei, Yuanyuan Hu*, Junping Yu*. Evaluation of a direct phage DNA detection-based Taqman qPCR methodology for quantification of phage and its application in rapid ultrasensitive identification of Acinetobacter baumannii. BMC Infectious Diseases. 2022 Jun 7;22(1):523. doi: 10.1186/s12879-022-07493-1. PMID: 35672689; PMCID: PMC9172196.
14. Wenhao Zhou, Ping He, Junhua Li, Huan Liu, Mengjuan Shi, Junping Yu*, Hongping Wei*. Steep Decline in Binding Capability of SARS-CoV-2 Omicron Variant (B.1.1.529) RBD to the Antibodies in Early COVID-19 Convalescent Sera and Inactivated Vaccine Sera. Viruses. 2022; 14(2):335. https://doi.org/10.3390/v14020335
15. Dongyan Xiong, Xiaoxu Zhang, Mengjuan Shi, Nuo Wang, Ping He, Zhuo Dong, Jie Zhong, Jing Luo, Yong Wang, Junping Yu*, Hongping Wei*. Developing an Amplification Refractory Mutation System-Quantitative Reverse Transcription-PCR Assay for Rapid and Sensitive Screening of SARS-CoV-2 Variants of Concern. Microbiol Spectr. 2022 Jan 5:e0143821.
16. Dongyan Xiong, Xiaoxu Zhang, Jin Xiong, Junping Yu*, Hongping Wei*. Rapid genome-wide sequence typing of African swine fever virus based on alleles. Virus Res. 2021 May;297:198357.
17. Dongyan Xiong, Caroline Muema, Xiaoxu Zhang, Xinming Pan, Jin Xiong, Hang Yang, Junping Yu*, Hongping Wei*. Enriched Opportunistic Pathogens Revealed by Metagenomic Sequencing Hint Potential Linkages between Pharyngeal Microbiota and COVID-19. Virol Sin. 2021 Oct;36(5):924-933.
18. Huan Liu, Ping He, Fei Meng, Mengwei Jiang, Jin Xiong, Junhua Li, Junping Yu*, Hongping Wei*. A Semi-automated Luciferase Immunoprecipitation Assay for Rapid and Easy Detection of African Swine Fever Virus Antibody. J Clin Microbiol. 2021 Sep 20;59(10):e0099021.
19. Fengyun Li, Ping He, Dongyan Xiong, Yakun Lou, Qiaosheng Pu, Haixia Zhang, Huige Zhang, Junping Yu*. A Reverse Transcription Recombinase-Aided Amplification Method for Rapid and Point-of-Care Detection of SARS-CoV-2, including Variants. Viruses. 2021 Sep 19;13(9):1875.
20. Yun Zhang, Wenqing Tan, Yang Zhang, Huili Mao, Shuyou Shi, Liangwei Duan, Hui Wang, Junping Yu*. Ultrasensitive and selective detection of Staphylococcus aureus using a novel IgY-based colorimetric platform. Biosensors & bioelectronics 2019, 142:111570.
21. Jun Luo, Mengwei Jiang, Jin Xiong, Junhua Li, Xiaoxu Zhang, Hongping Wei*, Junping Yu*. Exploring a phage-based real-time PCR assay for diagnosing Acinetobacter baumannii bloodstream infections with high sensitivity. Analytica Chimica Acta, 2018, 1044, 147-153.
22. Tao Jia, Zhi-Hang Chen, Peng Guo, Junping Yu*. An insight into DNA binding properties of newly designed cationic δ,δ′‑diazacarbazoles: Spectroscopy, AFM imaging and living cells staining studies. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2019, 211, 15 March 2019, 260-271. (IF=2.84)
23. Yun Zhang, Chenghui Yan, Hang Yang, Junping Yu*, Hongping Wei*, Rapid and selective detection of E. coli O157:H7 combining phagomagnetic separation with enzymatic colorimetry, Food Chem 234 (2017) 332-338.
24. Chenghui Yan, Yun Zhang, Hang Yang, Junping Yu*, Hongping Wei*, Combining phagomagnetic separation with immunoassay for specific, fast and sensitive detection of Staphylococcus aureus, Talanta 170 (2017) 291-297.
25. Yun Zhang, Hang Yang, Junping Yu*, Hongping Wei*. Rapid and sensitive detection of HIV-1 p24 antigen by immunomagnetic separation coupled with catalytic fluorescent immunoassay. Anal Bioanal Chem (2016) 408:6115–6121.
26. Junping Yu, Yun Zhang, Yun Zhang, Heng Li, Hang Yang, Hongping Wei*. Sensitive and rapid detection of Staphylococcus aureus in milk via cell binding domain of lysin. Biosensor and Bioelectronics. 2016, 77, 366-371.
27. Jianli Liu,  Xuejie Zhang, Hang Yang, Jinghe Yuan, Hongping Wei, Junping Yu* and Xiaohong Fang*. Study of the interactions between endolysin and bacterial peptidoglycan on S. aureus by dynamic force spectroscopy. Nanoscale. 2015, 7, 15245-15250. (SCI, IF=7.39)
28. Tao Jia, Jing Wang, Peng Guo and Junping Yu*. Characterizations of cationic γ-carbolines binding with double-stranded DNA by spectroscopic methods and AFM imaging. Org. Biomol. Chem., 2015, 13,1234-1242. (SCI, IF=3.562)
29. Tao Jia, Jin Xiang, Jing Wang, Peng Guo and Junping Yu*. Interactions of newly designed dicationic carbazole derivatives with double-stranded DNA: syntheses, binding studies and AFM imaging. Org. Biomol. Chem., vol.11, 2013, pp. 5512-5520. (SCI, IF=3.562)
30. Junping Yu, Mirong Guan, Fengyun Li, Zhiping Zhang, Chunru Wang, Chunying Shu*, Hongping Wei* and Xian-En Zhang*. Effects of fullerene derivatives on bioluminescence and application for protease detection. Chem Comm, vol.48, 2012, pp.11011–11013. (SCI, IF=6.834)
31. Junping Yu, Julia Warnke, Yuri L. Lyubchenko. Nanoprobing of α-synuclein misfolding and aggregation with AFM. Nanomedicine: Nanotechnology, Biology and Medicine, 2011, 7, 146-152. (SCI, IF=6.155)
32. Tieshan Teng, Junping Yu, Hang Yang, Hongping Wei*. Isolation and Complete Genome Sequence of a Novel Virulent Mycobacteriophage, Casbig. Virol Sin 2015, 30, 76. (SCI)
33. Fengyun Li, Junping Yu, Zhiping Zhang, Zongqiang Cui, Dianbing Wang, Hongping Wei* and Xian-En Zhang*. Use of hGluc/tdTomato pair for sensitive BRET sensing of protease with high solution media tolerance. Talanta, vol.109, 2013, pp.141-146. (SCI, IF=3.545)
34. Fengyun Li, Junping Yu, Zhiping Zhang, Zongqiang Cui, Dianbing Wang, Hongping Wei*, Xian-en Zhang*. Buffer Enhanced Bioluminescence Resonance Energy Transfer Sensor Based on Gaussia Luciferase for in-vitro Detection of Protease. Analytica Chimica Acta, vol.724, 2012, pp.104-110. (SCI, IF=4.513)
35. Yue Wang, Fang Wang, Junping Yu, Feng-Chao Jiang, Xin-Lei Guan, Can-Ming Wang, Lei Li, Hui Cao, Ming-Xing Lia, Jian-Guo Chen*. Novel multipotent phenylthiazole-tacrine hybrids for the inhibition of cholinesterase activity, β-amyloid aggregation and Ca²⁺ overload. Bioorganic & Medicinal Chemistry, 2012, 20, 6513-22. (SCI, IF=2.793)
36. Qingye Liu, Xiaojuan Xu, Lina Zhang, Junping Yu. Interaction between polydeoxyadenylic acid and beta-glucan from Lentinus edodes. European Polymer Journal, 2012, 48, 1329–1338. (SCI, IF=3.005)
37. Zhiguo Wu, Tian Tian, Junping Yu, Xiaocheng Weng, Yi Liu, Xiang Zhou*. Formation of Sequence-Independent Z-DNA Induced by a Ruthenium Complex at Low Salt Concentrations. Angew Chem Int Edit. 2011, 50, 11962-11967. (SCI, IF=11.261)

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My research interests are focused on detecting pathogens rapidly and sensitively by using some advanced techniques or nanomaterials, controlling pathogens by combining nanomaterials and bacteriophage or related lysins.

已指导学生

王孟月  02  63228  

现指导学生

严城辉  02  19178